US3924500A - Device in a microtome for holding a grid on which sections are collected - Google Patents

Device in a microtome for holding a grid on which sections are collected Download PDF

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Publication number
US3924500A
US3924500A US499913A US49991374A US3924500A US 3924500 A US3924500 A US 3924500A US 499913 A US499913 A US 499913A US 49991374 A US49991374 A US 49991374A US 3924500 A US3924500 A US 3924500A
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US
United States
Prior art keywords
grid
knife
holder
sections
grid holder
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US499913A
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English (en)
Inventor
Erik Lennart Kindel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Reichert Jung Optische Werke AG
Pfizer Health AB
Original Assignee
LKB Produkter AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LKB Produkter AB filed Critical LKB Produkter AB
Application granted granted Critical
Publication of US3924500A publication Critical patent/US3924500A/en
Assigned to REICHERT-JUNG OPTISCHE WERKE A.G. reassignment REICHERT-JUNG OPTISCHE WERKE A.G. ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: LBK-PRODUKTER AB
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T83/00—Cutting
    • Y10T83/222—With receptacle or support for cut product

Definitions

  • the present invention relates to a device in a microtome in which a specimen is sectioned in thin sections by a knife, the knife being arranged in a knife attachment, for collecting of sections obtained.
  • a knife consisting of a glass prism.
  • the knife is usually arranged in a knife attachment, comprising two rectangular members situated on each side of the glass prism.
  • a knife attachment comprising two rectangular members situated on each side of the glass prism.
  • the parallel members of the knife attachment in combination with a suitably attached tape strip, form a trough which is filled with a liquid onto which the achieved successive sections are made to float.
  • the sections may after completed sectioning be transferred to a so-called grid, that is a circular copper net, for investigation in an electron microscope.
  • a so-called grid that is a circular copper net
  • the present invention relates to a device by means of which the sections are caught up directly on the grid which is designed for later investigation of the sections in an electron microscope.
  • the grid may be moved to that location along the knife edge where the most effective sectioning is obtained or to that position along the knife edge where the most interesting part of the specimen will hit the knife edge.
  • the sections caught up on the grid may rapidly be removed for the smallest possible temperature rise during this operation step.
  • FIG. 1 shows a perspective sketch of a knife and a knife attachment in a microtome
  • FIG. 2 shows a perspective sketch of a device according to the invention, viewed from below;
  • FIG. 3 shows a perspective sketch of the same device seen from above.
  • the number 1 denotes a knife consisting of a triangular glass prism having the knife edge 2
  • 3 and 4 denote two rectangular members forming parts of the knife attachment.
  • a wall can 'be inserted so that the knife attachment will form a trough which may be filled with a liquid.
  • the grid holder according to a preferred embodiment of the invention comprises a middle plate 6 having an upper plate 7 slidably applied on top of it and a block 8 attached under it. Between the plates 6 and 7 there is formed an aperture 9 in which a grid 10 can be inserted.
  • the number 11 denotes two bored holes by means of which the grid holder and grid can easily be handled by means of a pair of forceps.
  • the sliding displacement of the upper plate 7 is governed by the pins 12 in the openings 13.
  • the device according to the invention works in the following way:
  • the grid holder is instead positioned onto the upper ridges of the rectangular members 3 and 4, whereby the plate 6 of the grid holder will rest upon these.
  • the purpose of the block 8 is to exert guiding action against the rectangular members 3 and 4, the grid holder then being displaceable in direction towards the knife edge 2, essentially perpendicularly against this or in an angle determined by the design of the knife attachment.
  • the grid 10 can easily be inserted into the aperture 9.
  • the block 8 can, of course, be substituted by two governing blocks. It is preferred that the rectangular members 3 and 4 have the same height whereby the aperture 9 will be horizontal.
  • the grid holder with the grid 10 is displaced towards the knife in order that the grid 10 is brought in contact with the knife in close vicinity to the knife edge 2.
  • the aperture 9 then should have such a length that the grid 10 can be displaced laterally to that point where the knife edge is sharpest or to that point where the most interesting part of the specimen is hitting the knife edge.
  • the grid holder can simply be lifted by means of a pair of forceps by the bored holes 11. Alternatively gripping means for rapid handling by means of a pair of forceps should not fall outside the scope of the invention.
  • the grid holder is in collecting position in the vicinity of the knife edge the upper plate 7 is in its rear position as shown in the figures.
  • the grid holder and grid are lifted away and the grid should be removed from the grid holder the upper plate 7 is simply displaced in the direction of the grid so as to push it off from the grid holder.
  • the aperture 9 may alternatively be made in a simpler way.
  • the upper plate 7 may be dispensed with and the aperture 9 be designed as a slit in the plate 6.
  • a portion of the plate 6 then could be bent for horizontal insertion of the grid 10.
  • the upper ridges of the rectangular members 3 and 4 usually are horizontal but could also incline by a small angle.
  • the aperture 9 can be designed in order that the grid 10 is inserted in the aperture in a plane parallel to the plane of the plate 6.
  • the invention also embraces devices where the grid is forming an angle against the plate 6.
  • the grid holder could instead by provided with suitable attachment, providing for the grid keeper to 3 move directly towards the knife.
  • Device for use in microtomes of the type wherein thin sections of material cut by a knife means are to be collected on a grid comprising a grid holder, means for mounting the grid holder on said knife means for movement toward and away from the knife edge of said knife means in the plane within which said knife edge is contained, said grid holder being provided with a recess facing said knife edge for removably supporting a grid close to the knife edge to collect specimens cut by the knife means.
  • said means for mounting the grid holder includes a knife holder having spaced walls provided with generally horizontal upper margins, said grid holder being slidable on said upper margins.
  • said grid holder comprises a flat element having guide means on the lower surface thereof for sliding engagement with the inner surfaces of the spaced walls, said recess extending across the width of one margin of said element.
  • said grid holder comprises a pair of rectangular flat members connected together for relative slidable adjustment, said recess being formed in the end margin of at least one of said members.

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
US499913A 1973-08-28 1974-08-23 Device in a microtome for holding a grid on which sections are collected Expired - Lifetime US3924500A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE7311639A SE371886B (de) 1973-08-28 1973-08-28

Publications (1)

Publication Number Publication Date
US3924500A true US3924500A (en) 1975-12-09

Family

ID=20318342

Family Applications (1)

Application Number Title Priority Date Filing Date
US499913A Expired - Lifetime US3924500A (en) 1973-08-28 1974-08-23 Device in a microtome for holding a grid on which sections are collected

Country Status (6)

Country Link
US (1) US3924500A (de)
JP (1) JPS5064885A (de)
AT (1) AT336920B (de)
FR (1) FR2242677B1 (de)
GB (1) GB1479927A (de)
SE (1) SE371886B (de)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0088549A1 (de) * 1982-03-04 1983-09-14 TECHNICON INSTRUMENTS CORPORATION (a New York corporation) Schneidevorrichtung und Verfahren und Vorrichtung zur Schnittunterstützung
US5050470A (en) * 1989-05-15 1991-09-24 Ward John L Receiver for specimens from cryogenic microtome
US5551326A (en) * 1994-12-15 1996-09-03 Wisconsin Alumni Research Foundation Adhesive-less microtome boat
US20090183613A1 (en) * 2008-01-18 2009-07-23 Leica Mikrosysteme Gmbh Micromanipulator for a cryomicrotome

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57116843U (de) * 1981-01-13 1982-07-20

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3225639A (en) * 1964-02-21 1965-12-28 Martinelli Robert Microtome knife
US3377898A (en) * 1966-03-21 1968-04-16 Lkb Produkter Aktiebolag Microtome provided with microscope and specimen catching grid operated thereby
US3460417A (en) * 1967-07-17 1969-08-12 Miles Lab Microtome

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3225639A (en) * 1964-02-21 1965-12-28 Martinelli Robert Microtome knife
US3377898A (en) * 1966-03-21 1968-04-16 Lkb Produkter Aktiebolag Microtome provided with microscope and specimen catching grid operated thereby
US3460417A (en) * 1967-07-17 1969-08-12 Miles Lab Microtome

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0088549A1 (de) * 1982-03-04 1983-09-14 TECHNICON INSTRUMENTS CORPORATION (a New York corporation) Schneidevorrichtung und Verfahren und Vorrichtung zur Schnittunterstützung
US5050470A (en) * 1989-05-15 1991-09-24 Ward John L Receiver for specimens from cryogenic microtome
US5551326A (en) * 1994-12-15 1996-09-03 Wisconsin Alumni Research Foundation Adhesive-less microtome boat
US20090183613A1 (en) * 2008-01-18 2009-07-23 Leica Mikrosysteme Gmbh Micromanipulator for a cryomicrotome
US10520398B2 (en) 2008-01-18 2019-12-31 Leica Mikrosysteme Gmbh Micromanipulator for a cryomicrotome

Also Published As

Publication number Publication date
AT336920B (de) 1977-06-10
GB1479927A (en) 1977-07-13
DE2440813A1 (de) 1975-04-10
FR2242677B1 (de) 1979-01-05
ATA693274A (de) 1976-09-15
DE2440813B2 (de) 1977-01-20
FR2242677A1 (de) 1975-03-28
SE371886B (de) 1974-12-02
JPS5064885A (de) 1975-06-02

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Legal Events

Date Code Title Description
AS Assignment

Owner name: REICHERT-JUNG OPTISCHE WERKE A.G., HERNALSER HAUPT

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:LBK-PRODUKTER AB;REEL/FRAME:004943/0611

Effective date: 19880615

Owner name: REICHERT-JUNG OPTISCHE WERKE A.G.,AUSTRIA

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:LBK-PRODUKTER AB;REEL/FRAME:004943/0611

Effective date: 19880615