US3834874A - Detection of malarial parasites in blood - Google Patents

Detection of malarial parasites in blood Download PDF

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Publication number
US3834874A
US3834874A US00298549A US29854972A US3834874A US 3834874 A US3834874 A US 3834874A US 00298549 A US00298549 A US 00298549A US 29854972 A US29854972 A US 29854972A US 3834874 A US3834874 A US 3834874A
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US
United States
Prior art keywords
blood
stained
parasites
methylene blue
malarial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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US00298549A
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English (en)
Inventor
S Gottlieb
J Geating
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General Electric Co
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General Electric Co
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Filing date
Publication date
Application filed by General Electric Co filed Critical General Electric Co
Priority to US00298549A priority Critical patent/US3834874A/en
Priority to JP11642873A priority patent/JPS5510030B2/ja
Application granted granted Critical
Publication of US3834874A publication Critical patent/US3834874A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • This invention pertains to methods of biological diagnostic observation, and in particular to a method of identifying the presence of malarial parasites in human blood.
  • Neutrophils contain a finely granulated cytoplasm which stains a light purple in the fresh preparation and fades to a greenish tint in the older smear. Nuclei stain a bright purple.
  • Eosinophils exhibit a bright purple nucleus and a cytoplasm packed with large uniform orange-staining granules which fade to a greenish orange when the preparation is about twelve hours old.
  • Basophils contain smaller number of uniform-size granules than eosinophils, which stain a dark purple and often completely obscure the nucleus. With focusing, an orange tinge can be observed in the granules found at the edge of the cell, an effective identifying feature.
  • Lymphocytes develop a purple staining nucleus surrounded by a lighter purple cytoplasm which may become as dark as the nucleus as the preparation grows old.
  • Monocytes have an affinity for the stain similar to lymphocytes. Distinguishing features are the larger size and the greater amount of cytoplasm of the monocytes. It has been found that the differentiation is readily learned.
  • Reticulocytes contain a reddish-purple network but do not fade even after the red cells become distorted.
  • the reticulocyte count should also be made within 4 to 5 hours after the staining period because of the fading of some of the red blood corpuscles when they die.
  • Trophozoites both early and late, were observed in the smears of both individuals.
  • the cytoplasm was slightly pink in color.
  • the trophozoites appeared more spherical and smaller in size as compared to the standard method in which they appeared larger and more ovoid.
  • many trophozoites appeared ovoid as in a conventional preparation but they did, however, appear smaller.
  • gametocytes were found to be present. However, they did not have the typical crescent appearance of gametocytes which appear in Falciparum Malaria.
  • the gametocytes were mostly ovoid in shape with dense staining chromatin material at one pole.
  • gametocytes were dumb-bell shaped with the dense staining chromatin material in the middle.
  • the utilization of prestained slides for recognition and identification of malarial parasites has the following advantages over the existing method:
  • the method of identifying malarial parasites in human blood which comprises:
  • the method of differentially staining malarial parasites in human blood which comprises applying a sample of blood containing such parasites to a surface bearing a dried mixture of 91 to 94 weight percent Methylene Blue NN and 6 to 9 weight percent Cresyl Violet Acetate.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
US00298549A 1972-10-18 1972-10-18 Detection of malarial parasites in blood Expired - Lifetime US3834874A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US00298549A US3834874A (en) 1972-10-18 1972-10-18 Detection of malarial parasites in blood
JP11642873A JPS5510030B2 (cg-RX-API-DMAC10.html) 1972-10-18 1973-10-18

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US00298549A US3834874A (en) 1972-10-18 1972-10-18 Detection of malarial parasites in blood

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US3834874A true US3834874A (en) 1974-09-10

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US00298549A Expired - Lifetime US3834874A (en) 1972-10-18 1972-10-18 Detection of malarial parasites in blood

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US (1) US3834874A (cg-RX-API-DMAC10.html)
JP (1) JPS5510030B2 (cg-RX-API-DMAC10.html)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3928141A (en) * 1974-03-12 1975-12-23 Int Diagnostic Products Ltd Method and apparatus for nitroblue tetrazolium (NBT) tests for chronic granulomatous disease and related syndromes
DE2737845A1 (de) * 1975-08-08 1978-03-02 Gen Electric Verfahren zum anfaerben und versiegeln einer biologischen probe, die an einem mikroskopischen objekttraeger haftet und luftgetrocknet ist, insbesondere einer blutprobe
US4224277A (en) * 1975-04-11 1980-09-23 Boehringer Mannheim Gmbh Coated slides and apparatus for coating same
WO2013038306A1 (en) * 2011-09-13 2013-03-21 Koninklijke Philips Electronics N.V. System and kit for preparing a cytological sample for examination
EP4118419A4 (en) * 2020-03-13 2024-03-13 Advanced Animal Diagnostics, Inc. FAST MOBILE SCREENING AND TRIAGE FOR INFECTIONS AND SERIOUS INFECTIONS

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3928141A (en) * 1974-03-12 1975-12-23 Int Diagnostic Products Ltd Method and apparatus for nitroblue tetrazolium (NBT) tests for chronic granulomatous disease and related syndromes
US4224277A (en) * 1975-04-11 1980-09-23 Boehringer Mannheim Gmbh Coated slides and apparatus for coating same
DE2737845A1 (de) * 1975-08-08 1978-03-02 Gen Electric Verfahren zum anfaerben und versiegeln einer biologischen probe, die an einem mikroskopischen objekttraeger haftet und luftgetrocknet ist, insbesondere einer blutprobe
WO2013038306A1 (en) * 2011-09-13 2013-03-21 Koninklijke Philips Electronics N.V. System and kit for preparing a cytological sample for examination
EP4118419A4 (en) * 2020-03-13 2024-03-13 Advanced Animal Diagnostics, Inc. FAST MOBILE SCREENING AND TRIAGE FOR INFECTIONS AND SERIOUS INFECTIONS

Also Published As

Publication number Publication date
JPS5510030B2 (cg-RX-API-DMAC10.html) 1980-03-13
JPS4973881A (cg-RX-API-DMAC10.html) 1974-07-17

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