US3810720A - Apparatus for transferring liquids - Google Patents

Apparatus for transferring liquids Download PDF

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Publication number
US3810720A
US3810720A US00252640A US25264072A US3810720A US 3810720 A US3810720 A US 3810720A US 00252640 A US00252640 A US 00252640A US 25264072 A US25264072 A US 25264072A US 3810720 A US3810720 A US 3810720A
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US
United States
Prior art keywords
conduit
valve means
conduit system
pump
portions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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US00252640A
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English (en)
Inventor
G Lartigue
A Rousselet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Hoffmann La Roche Inc
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F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
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Publication of US3810720A publication Critical patent/US3810720A/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G05—CONTROLLING; REGULATING
    • G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
    • G05D11/00—Control of flow ratio
    • G05D11/001—Control of flow ratio with discontinuous action
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G01N2001/382—Diluting, dispersing or mixing samples using pistons of different sections

Definitions

  • the present invention relates to a device for transferring predetermined quantities of different liquid medium contained in separate containers which device comprisespump means for selectively withdrawing predetermined amounts of different liquid media from different containers and then transferring the withdrawn liquid media to the same receptacle.
  • a specimen sample such as urea, blood, sputa and the like
  • ready means be found for withdrawing from one container, a predetermined amount of a liquid designed to biochemically react with specimen sample and from another container predetermined amounts of said specimen sample and then combining the two in a single vessel. In this way, diagnostically significant indications can be revealed.
  • the apparatus which constitutes the present invention is adapted to perform such an operation or a succession of such operations.
  • a particularly noteworthy feature of the apparatus of the present invention resides in its capability of permitting the amount of liquid medium that need be withdrawn from each vessel to be varied at will, in accordance with established criteria.
  • the apparatus according to the present invention is characterized in that it comprises two pumps provided by a piston meansmounted in sequence in a system or circuit means.
  • the circuit means is adopted during one phase of operation to withdraw liquid media contained in separate receptacles in communicating with the circuit means and in another phase of operation, to discharge all of the liquid media withdrawn from the separate receptacles into a single vessel.
  • the amount of liquid drawn to the system or circuit means can be readily and conveniently varied depending upon the liquid medium utilized and the result sought.
  • the invention is further characterized in that means are provided for independently regulating the means which control the amount of liquid medium to be drawn into the system or circuit means from the container in which a particular medium is contained.
  • an object of the present invention is to provide a device which permits the ready and facile transfer of predetermined quantities of different liquid media contained in separate vessels into a single vessel.
  • FIG. 1 is a diagram of apparatus according to the invention.
  • FIG. 2 is a partial rear view of the apparatus.
  • FIG. 3 is a view in vertical section of the apparatus according to the invention.
  • FIG. 4 is a plan view of a cam.
  • FIG. 5 is a view in section on the line 5-5 of FIG. 4.
  • FIG. 6 is a diagram of part of the apparatus.
  • FIG. 7 is a perspective view of a component of the apparatus.
  • FIG. 8 is a front elevation of the apparatus according to the invention.
  • FIG. 9 is a diagram of the operation sequence of the apparatus.
  • the device as shown in FIG. 1 comprises a block 10 advantageously formed from a transparent plastic.
  • Block 10 is provided with two cylindrical bores 11 and 12.
  • the longitudinal axis of bores 11 and 12 are parallel and of the same length, while the transverse axis of each of bore 11 and bore 12 are different. It is, of course, to be understood that the dimensions of bores 11 and 12 can be varied.
  • plungers or cylinders 13 and 14 Disposed slidably in bores 11 and 12 are plungers or cylinders 13 and 14 respectively which comprises a first and second pump means.
  • Each plunger 13 and 14 is closely fitted in bores l l and 12.
  • a gap of the order of a millimeter exists between the pistons, and the walls (e.g. wall 15), in block 10 which define bores 11 and 12. While this value is preferred, it is not to be deemed limitative.
  • a seal, advantageously, a removable sealing ring 17 provides sealing tightness when the plunger 13 is disposed in the bore 11 in accordance with conventional practice.
  • Chamber 21 of the first pump means is provided by the space in bore 11 not occupied by the piston 13. Chamber 21 is in communication with chamber 24. Chamber 24 is provided by the space in bore 12 left vacant by piston 14. Communication between chambers 21 and 24 is provided by a conduit 22 extending from the top part 23 of chamber 21 to an orifice 27 formed in the bottom part of the wall defining bore 12. Piston 14 has disposed about the lower portion thereof a sealing means 26 which may be a removable ring similar to removable ring 17.
  • Conduit 22 which may be any suitable hollow flexible tube means includes a flexible portion 27 which is capable of being deformed or crimped whereby the open ing in the conduit 22 may be closed.
  • a crimping or pressing means 44 is provided for closing conduit 22.
  • Means 44 comprises a block portion 84 formed with an internal open portion 85.
  • Crimping or pressing means 44 is received in an opening in block 10 for vertical sliding movement. Movement of means 44 in a vertical direction is controlled by means of a stud which is received in a longitudinal slot 81 in means 44. Stud 80 is secured to with block 10.
  • crimping means 44 is provided with a triangular portion 28; the rounded apex of which extends downwardly into the open portion of the means 44.
  • a second triangular-shaped portion 41' is also provided.
  • the rounded apex of portion 41 extends upwardly into the said open portion of means 44.
  • Disposed between the apexes of portions 28 and 41' is a rigid member 83.
  • the upper and lower faces of rigid member 83 has formed thereon two dish-shaped members 29 and 31. The dish-shaped members face in opposite directions.
  • Flexible portion 27 is positioned in the open portion of crimping means 44 between members 28 and 29.
  • a second deformable conduit 32 is positioned in crimping means 44 between portions 31 and 41.
  • Conduit 32 (FIG. 1) is securely received in an opening on the bottom part 34 of the wall which defines bore 10.
  • the other end of conduit 32 comprises a hollow needle 35 for withdrawing a liquid diluent, e.g. a specimen sample in solution form, from a vessel 36.
  • conduit 38 extends from the top of bore 12.
  • Conduit 38 comprises any suitable tube means for a major part of its length.
  • conduit 38 At its free end, conduit 38 comprises a hollow needle means 39 and the conduit 38 is movable advantageously so that needle 39 can occupy either of the two positions 39 or 39; whereby it can be received in either a first vessel 40 or a second vessel 41 depending upon the phase of operation of the device.
  • Cam 42 comprises a part of a disc 51 (FIGS. 4-6) in which a stud 54 is mounted.
  • the cam disc 51 has integral therewith at its upper end, the cam 42 and the stud 54.
  • a roller 54' On stud 54, is a roller 54' (FIGS. 3 and 6).
  • Roller 54 is disposed in a horizontal slot in a member 55 secured to a sleeve 45.
  • Sleeve 45 is slidably positioned on a vertical shaft 46 secured to the apparatus frame 47.
  • a plate member 56 is integrally connected to sleeve 45. Plate member 56 is adopted to move sleeve 45 along its vertical axis.
  • Plate member 56 has formed therein two indentations 57 and 58, which have arcuately shaped bottoms 59 and 59'.
  • Rods 60 and 61 as seen in FIG. 8 are fixedly secured, respectively, to piston means 13 and 14.
  • Rods 60 and 61 are slidably disposed in the indentations 57 and 58 on plate means 56.
  • Rods 60 and 61 are respectively provided with shoulders 62 and 63 which bear upon the top surface 64 of plate member 56 in one position of operation.
  • stops 67 and 70 may include bands about rods 60 and 61 which bands when loosened by the knurled knob shown in FIG. 3 can be moved upwardly or downwardly on rods 60 and 61. When a desired position is reached as indicated on the scale or indicia means, the band is tightened about rods 60 and 61. Then relative sliding movement between respectively rods 60 and 61 and stops 67 and 70 is precluded.
  • stops 67 and 70 may include bands about rods 60 and 61 which bands when loosened by the knurled knob shown in FIG. 3 can be moved upwardly or downwardly on rods 60 and 61.
  • the band is tightened about rods 60 and 61. Then relative sliding movement between respectively rods 60 and 61 and stops 67 and 70 is precluded.
  • it should be apparent other arrangements can also be employed in lieu of the aforementioned bands.
  • the apparatus additionally comprises an electric motor M, the shaft of which forms the input of a coupling and breaking device and also comprises means which via an electric signal provided by a programmer or any logic device actuates the coupling and breaking device under the influence of a current impulse. Actuation of motor means M initiates rotation of disc 51 in accordance with conventional practice. Means are provided for stopping the device, which means are controlled by the programmer or logic device.
  • a button 81 can be used for switching over from automatic operation to manual operation, in which the starting and stopping are under manual control.
  • a switch 82 (FIG. 3) is provided to initiate operation of the apparatus when its moving parts are in a predetermined position in accordance with conventional procedures.
  • the operation of the device is as follows.
  • the hydraulic circuit assembly including the pump chambers is first filled with liquid, eg. a physiological solution, eg. sterile water and the like.
  • liquid eg. a physiological solution, eg. sterile water and the like.
  • member 56 which initially is in the uppermost position thereof is driven downwardly in a vertical direction by means of roller 54'.
  • Roller '54 which is fixedly secured to disc 51 moves as a consequence of disc 51 being rotated under the influence of the shaft of the motor means M.
  • the movement of member 56 in a vertical direction is controlled by the sliding movement of sleeve 45 on shaft 46.
  • cam 42 on disc 51 moves out of engagement with roller 43. Pressing or crimping means 44 is then biased downwardly under the influence of the spring means 30.
  • the amount of movement of pistons 13 and 14 and hence the amount of liquid taken into each chamber can be controlled.
  • the importance of this should be readily apparent to one skilled in the diagnostic art, because when doing analysis for diagnostically significant indications, for example, it is often desirable that a certain aliquot of specimen sample should be combined with a certain aliquot of reagent medium in order to assure that diagnostically significant indications be observed.
  • the rotation of cam 51 is stopped.
  • the stopping is such that the amount of liquid drawn into the chambers is the amount indicated on the indicia or scale means by the pointer before operation.
  • the pistons rise simultaneously and since the orifice in conduit 32 is closed, all of the liquid contained in the chambers 21 and 24 is forced via conduits 22 and 38 into receptacle 41 which has now taken the place of vessel 40.
  • the liquid medium withdrawn from vessel 40 during the first stage is first transferred into vessel 41. Its entry into vessel 41 is followed by a predetermined amount of liquid medium equal to the quantity withdrawn from vessel 36 during the first stage.
  • the liquid medium in vessel 41 comprises both the liquid which has been withdrawn from vessel 40 and from vessel 36, e.g. centrifuged total blood, some globuals of which are withdrawn for dilution with physiological serum in a proportion defined by the positions of stops 70 and 67.
  • the link cooperating with roller 54' insures that the conduits which include pipes 27 and 32 are moved into engagement with portions 28 and 41' only when the pistons are stationery.
  • the tubes comprising the conduit may be formed of any elastomeric material suitable for the purposes of the present invention.
  • the bottom part of FIG. 9 shows valves V and V when open and closed respectively.
  • the diagram 74 at the top part of FIG. 9 shows how valve operation is controlled..Positions 75 and 76 corresponding to the bottom and top dead center positions respectively of roller 54'. The angle between positions 75 and 76 correspond to the movement of roller 54'.
  • Each operating cycle is initiated by a signal from the programmer which may be any suitable logic device.
  • knob 81 is set to manual operation resulting in the continuance sequence of operating cycles in which valves V and V successfully take up the position shown in the bottom of H6. 9.
  • the apparatus operates without damage even in the absence of liquid.
  • the volume of either pump may be modified by replacing the pistons and the corresponding ring seals.
  • An apparatus for transferring quantities of different liquid media contained in separate liquid receiving vessels into a single vessel which comprises a conduit system having open ends, two piston pump means, each 6 including a plunger portion and a pump casing in which the plunger portion is slidably received, disposed sequentially in the conduit system between the open ends, a first conduit portion of said conduit system connecting said pump means, a first valve means in the conduit system between the said two pump means for opening and closing the first conduit portion, a second conduit portion in the conduit system being secured to one of said pump means, said second conduit portion including one of said open ends of the conduit system, a third conduit portion in the conduit system secured to the other of said pumps, said third conduit portion including the other of said open ends of the conduit sys tem, second valve means in the conduit system positioned in the conduit system between one of said pump means and said one of said open ends for cooperation with the second conduit portion whereby said second valve means can effect the opening and closing of said second conduit portion and means movable by a motor means operatively connected to the piston pumps and
  • first and second'conduit portions are formed from an elastomeric material and the opening and closing thereof by the first and second valve means, respectively, is effected by crimping the said conduit portions.
  • each of the valve means comprises a stationary support member and a movable portion, each of said conduit portions being disposed between a stationary support member and a movable portion.

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Automation & Control Theory (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
US00252640A 1971-05-18 1972-05-12 Apparatus for transferring liquids Expired - Lifetime US3810720A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7117955A FR2137308B1 (en:Method) 1971-05-18 1971-05-18

Publications (1)

Publication Number Publication Date
US3810720A true US3810720A (en) 1974-05-14

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US00252640A Expired - Lifetime US3810720A (en) 1971-05-18 1972-05-12 Apparatus for transferring liquids

Country Status (7)

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US (1) US3810720A (en:Method)
BE (1) BE783569A (en:Method)
CH (1) CH533463A (en:Method)
DE (1) DE2224385A1 (en:Method)
FR (1) FR2137308B1 (en:Method)
IT (1) IT955247B (en:Method)
NL (1) NL7206198A (en:Method)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3955930A (en) * 1975-04-07 1976-05-11 Justin Joel Shapiro Automatic dilutor having coupled diluent and reagent plungers
US4269327A (en) * 1978-05-24 1981-05-26 Welch Henry H Device for carrying out high precision dilutions and dosages of liquids, particularly biological liquids
US4976546A (en) * 1989-05-12 1990-12-11 Beattie Lewis J Two component fluid mixing and dispensing system
FR2836400A1 (fr) * 2002-02-25 2003-08-29 Junior Instruments Dispositif de pipetage automatique de precision
AU2001295706B2 (en) * 2000-10-24 2006-05-11 Junior Instruments Automatic pipetting device with rinsing

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3963148A (en) * 1974-01-10 1976-06-15 Coulter Electronics, Inc. Apparatus for drawing, measuring and discharging proportional amounts of fluid
GB1558931A (en) * 1976-06-03 1980-01-09 Brand Fa Rudolf Diluting and pipetting apparatus

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB487500A (en) * 1936-12-21 1938-06-21 Georg Ornstein Improved apparatus for dosing liquids and gases
US2815149A (en) * 1954-07-20 1957-12-03 Avery Hardoll Ltd Apparatus for dispensing liquids
US2922379A (en) * 1957-06-06 1960-01-26 Eugene L Schultz Heart action multi-line pump constructions
US3144831A (en) * 1961-07-13 1964-08-18 Beckman Instruments Inc Fluid gradient engine
US3230887A (en) * 1962-10-29 1966-01-25 Beckman Instruments Inc Even flow apparatus and method for fluid gradient engines and the like
US3359910A (en) * 1965-06-10 1967-12-26 Little Inc A Apparatus for programming fluid flow
US3401839A (en) * 1967-05-29 1968-09-17 Honeywell Inc Liquid sampling and dispensing device
US3484207A (en) * 1967-05-22 1969-12-16 American Optical Corp Liquid sampling-pipetting method and apparatus
US3496970A (en) * 1966-01-07 1970-02-24 Coulter Electronics Apparatus for the preparation of liquid mixtures
US3575161A (en) * 1968-03-07 1971-04-20 Seymour B London Valve for biological systems

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3572130A (en) * 1969-08-27 1971-03-23 Nat Instr Lab Inc Liquid sample pick-up and dispensing apparatus

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB487500A (en) * 1936-12-21 1938-06-21 Georg Ornstein Improved apparatus for dosing liquids and gases
US2815149A (en) * 1954-07-20 1957-12-03 Avery Hardoll Ltd Apparatus for dispensing liquids
US2922379A (en) * 1957-06-06 1960-01-26 Eugene L Schultz Heart action multi-line pump constructions
US3144831A (en) * 1961-07-13 1964-08-18 Beckman Instruments Inc Fluid gradient engine
US3230887A (en) * 1962-10-29 1966-01-25 Beckman Instruments Inc Even flow apparatus and method for fluid gradient engines and the like
US3359910A (en) * 1965-06-10 1967-12-26 Little Inc A Apparatus for programming fluid flow
US3496970A (en) * 1966-01-07 1970-02-24 Coulter Electronics Apparatus for the preparation of liquid mixtures
US3484207A (en) * 1967-05-22 1969-12-16 American Optical Corp Liquid sampling-pipetting method and apparatus
US3401839A (en) * 1967-05-29 1968-09-17 Honeywell Inc Liquid sampling and dispensing device
US3575161A (en) * 1968-03-07 1971-04-20 Seymour B London Valve for biological systems

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3955930A (en) * 1975-04-07 1976-05-11 Justin Joel Shapiro Automatic dilutor having coupled diluent and reagent plungers
US4269327A (en) * 1978-05-24 1981-05-26 Welch Henry H Device for carrying out high precision dilutions and dosages of liquids, particularly biological liquids
US4976546A (en) * 1989-05-12 1990-12-11 Beattie Lewis J Two component fluid mixing and dispensing system
AU2001295706B2 (en) * 2000-10-24 2006-05-11 Junior Instruments Automatic pipetting device with rinsing
EP1328817B1 (fr) * 2000-10-24 2007-03-21 Junior Instruments Dispositif de pipetage automatique avec rincage
CN1313829C (zh) * 2000-10-24 2007-05-02 新式仪器公司 带有冲洗功能的自动吸液设备
FR2836400A1 (fr) * 2002-02-25 2003-08-29 Junior Instruments Dispositif de pipetage automatique de precision
WO2003073108A1 (fr) * 2002-02-25 2003-09-04 Stago Instruments Dispositif de pipetage automatique de precision
US20050079073A1 (en) * 2002-02-25 2005-04-14 Khaled Abou-Saleh Automatic precision pipetting device
US7470402B2 (en) 2002-02-25 2008-12-30 Stago Instrument Automatic precision pipetting device

Also Published As

Publication number Publication date
DE2224385A1 (de) 1972-11-30
NL7206198A (en:Method) 1972-11-21
FR2137308A1 (en:Method) 1972-12-29
FR2137308B1 (en:Method) 1975-01-17
CH533463A (de) 1973-02-15
IT955247B (it) 1973-09-29
BE783569A (fr) 1972-11-17

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