US3784448A - Diagnostic device - Google Patents

Diagnostic device Download PDF

Info

Publication number
US3784448A
US3784448A US00256984A US3784448DA US3784448A US 3784448 A US3784448 A US 3784448A US 00256984 A US00256984 A US 00256984A US 3784448D A US3784448D A US 3784448DA US 3784448 A US3784448 A US 3784448A
Authority
US
United States
Prior art keywords
media
innoculation
compartments
rod
compartment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US00256984A
Other languages
English (en)
Inventor
T Cekoric
P Yananton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Hoffmann La Roche Inc
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of US3784448A publication Critical patent/US3784448A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/801—Anerobic cultivation

Definitions

  • ABSTRACT Compartmented tube containing preprepared culture [22] Filed: May 25, 1972 the family Enterobacteriaceae. The improvement comprises inmedia for the differential identification of microorgan isms, particularly, organisms 0 I k m2 c JA c 9 3 l 5 9 l 4 m 8 m 6 n 5 u m M m 1 U w s A UI H nn 2 55 11. [.l
  • the present invention relates to an improved device for determining the presence of bacteria in a specimen sample.
  • Disposable diagnostic devices comprising several media-containing compartments and a wire rod extending through said compartments are known.
  • each compartment contains a conventional test medium which is suitable for determining the biochemical characteristics of an organism.
  • oneend of the rod is brought into contact with isolates obtained from a specimen sample which may be sputa, blood and the like.
  • the innoculation rod is then drawn through the various compartments so that any microorganisms contained on the said one end is a source of innoculum for the test media.
  • test media whichperform better under anaerobic conditions are affected by exposure to air.
  • Air may enter prior art devices via the space formerly occupied by the innoculation rod after it is withdrawn and/or may be present in the space above the media in the compartment in which it is contained.
  • a scored innoculation wire rod is provided. Upon severing the innoculation rod at its scored portion, the portion so severed can be reinserted intothose compartments which contain media adversly affected by aerobic conditions. Thereby, the entry of air into such compartments is limited.
  • overlay preferably a wax-overlay in cooperation with the scored portion can further assure the efficaciousness of media which performs better under anaerobic conditions.
  • FIG. 1 is a side view of a disposable diagnostic de vice.
  • FIG. 2 is a view of the elongated innoculation instrument.
  • FIG. 3 is'a view of the device after innoculation.
  • FIG. 4 illustrates an alternate rigid member utilizable for the purposes of the present invention.
  • the disposable diagnostic device is illustrated.
  • the device comprises a tubular member 2 made ofclear plastic.
  • the ends ofthe tubular member are secured to screw-type caps 3 and 4.
  • the tubular member is shown as being divided into eight compartments for receiving culture media indicated by dotted line. It is, of course, to be understood that the tubular member can comprise more or less than eight compartments.
  • each will contain a different culture media known to determine the presence of a particular organism.
  • each of the walls which define the compartments there is provided an opening.
  • Each of said openings are axially aligned whereby they can slidably receive a rod or wire member 5.
  • One end of the rod member 5 projects from one end of the tubular member and is provided with an innoculation means 6 which may be the end of the rod member 5.
  • the other end of the rod member 5 projects from the other end of the tubular member 2 and is provided with a handlemeans 7.
  • the rod member 5 is provided with ascored portion 8.
  • the innoculation means 6 is contacted with isolates obtained, for example, from urine, sputa and the like. After such contact, the handle means 7 is grasped by the user and the rod member 5 is pulled through the tubular member. As the innoculation means 6 passes through the compartments, it innoculates the media contained therein. After a suitable period, the container is visably examined for biochemical changes, evidenced by changes in characteristics of the media, e.g. change of color of the medium. Evidence of biochemical changes provides diagnostically significant information.
  • the disposable device contains media such as dextrose, lysine or ornithine or similar type media in compartments l0, l1 and 12.
  • media such as dextrose, lysine or ornithine or similar type media in compartments l0, l1 and 12.
  • the biochemical process occurs preferably in such type media under anaerobic conditions.
  • Compartments 10, 11 and 12 are disposed sequentially at the end of the device adjacent the handle means 7 and are each covered by a wax overlay 18.
  • the innoculation end 6 of rod member 5 is then reinserted into the tubular member 2.
  • the rod member 5 enters tubular member 2 via the centrally disposed openings contained in the compartment walls until its tip 9 passes into compartment 13.
  • Compartment 13 does not contain a media which is adversely affected by aerobic conditions, whereasas indicated above compartments l0, 1]. and l2 do.
  • the scored portion is about adjacent. the outer wall of compartment 10.
  • Rod member 5 is then broken at the scored portion. Then, the portion of the rod member 5 from the scored portion to the handle means 7 is discarded.
  • the remaining portion of the rod member 5 is now positioned in the openings in the walls which define compartments 10, 1'1 and 12 as seen in FIG. 3.
  • the openings in the walls defining com partments 10, 11 and 12 are closed.
  • The. improved device of the present invention provides a simple, reliable and rapid method for the identification of organisms, particularly, organisms of the Gram-negative bacteria of the family Enterobacteriaceae which is characteristic of prior art devices and yet improves upon the: prior art devices in that it avoids the problems inherent in the use of anaerobic test media inthe prior art devices.
  • the culture media found in the various compartments of the device may be selected from among the many suitable for the purposes of the present invention by a personskilled in the art.
  • test media All that is required of the test media is that it be a nutrient media which when innoculated with a particular type of bacteriaundergoes chemical changes which can be observed via the transparent compartment case. These changes permit the observation of diagnostically significant features.
  • a typical device will contain the following media: dextrose (compartment 10), lysine: (11) and ornithine (12) which biochemically react, preferably, in the absence of air.
  • the device may also contain H S-indole in compartment '13; lactose (14),PA-Dalcitol (l5), urea (16), citrate (17) or any suitable medium in any suitable number of compartments which biochemically react in the presence of air.
  • the score provided in rod member 5 provides a convenient means of insuring that media changes not be effected by aerobic conditions.
  • one of the advantages of the present invention is in the use of an innoculation rod that is scored and hence breakable, which innoculation rod can be broken and a portion thereof can be reinserted into the device to lessen the possibility of air entering those compartments which contain media, the biochemical changes of which would be adversely affected by the presence of air.
  • a protecting layer may be positioned thereon and in a preferred embodiment, a wax overlay is utilized.
  • the wax overlay overlies the media completely in the compartment and hence engages all of the four walls which define same.
  • a wax is preferred for the purposes of providing the overlay material, it is, of course, to be understood that other materials can also be efficaciously utilized.
  • other materials suitable for the purposes of the present invention can be included petroleum distillates such as vasoline, cosmoline and the like.
  • the overlay material should be pliable, should cover the media completely, should be non-porous and ifa wax, should free flow above about 65 C. and be solid below about 50 C.
  • the reinsertable portion can be between the handle 7 and the scored portion.
  • the rod 5 is removed, broken and then the portion containing the handle end is reinserted in the device. It is, of course, to be understood that in this embodiment, the portion between the handle end and the scored portion will be slightly greater than the sum of the distances between the walls which define compartments 10, 11 and 12.
  • the scored portion on the rod member may be eliminated and a separate rod 30 as seen in FIG. 4 can be provided for reinsertion into the tubular member.
  • compartments containing 3 anaerobic" and 5 aerobic" materials have been illustrated, it is, of course to be understood, that more or less than 8 compartments-can be employed and more or less than 3 anaerobic" media can be utilized. All that is required to accommodate any such modification is that the scored portion on rod 5 be so-situated thereon that when rod 5 is broken, the broken portion will pass through all the walls defining the compartments con taining anaerobic media (preferably coated with a wax overlay) and will not pass completely through compartment containing aerobic media.
  • a disposal diagnostic device which comprises a tubular member, said tubular member comprising several compartments, at least one of such compartments. containing a medium whose optimum performance is under anaerobic conditions, an innoculation member extending longitudinally through the said tubular member and including an innoculation end extending from one end of the tubular member, a handle end extending from the other end of the tubular member and a scored portion positioned between the handle end and the innoculation end, any compartment containing a media which biochemically reacts under anaerobic conditions being disposed adjacent to an endof the tubular member, the distance between the scored portion and one end of the innoculation member being slightly greater than the sum of the distances between the walls defining any compartment which contains a media which biochemically reacts under anaerobic conditions.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Examining Or Testing Airtightness (AREA)
US00256984A 1972-05-25 1972-05-25 Diagnostic device Expired - Lifetime US3784448A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US25698472A 1972-05-25 1972-05-25

Publications (1)

Publication Number Publication Date
US3784448A true US3784448A (en) 1974-01-08

Family

ID=22974412

Family Applications (1)

Application Number Title Priority Date Filing Date
US00256984A Expired - Lifetime US3784448A (en) 1972-05-25 1972-05-25 Diagnostic device

Country Status (18)

Country Link
US (1) US3784448A (xx)
JP (1) JPS5422035B2 (xx)
AT (1) AT330338B (xx)
AU (1) AU456457B2 (xx)
BE (1) BE799954A (xx)
CA (1) CA995569A (xx)
CH (1) CH582748A5 (xx)
CS (1) CS172256B2 (xx)
DD (1) DD106200A5 (xx)
DK (1) DK131581C (xx)
FR (1) FR2185380B1 (xx)
GB (1) GB1434092A (xx)
IL (1) IL42150A (xx)
IT (1) IT987390B (xx)
NL (1) NL156443B (xx)
NO (1) NO141656C (xx)
SE (1) SE389127B (xx)
SU (1) SU539540A3 (xx)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3960658A (en) * 1974-09-23 1976-06-01 Centaur Chemical Co. Multi-media petri dish
US4235963A (en) * 1979-05-11 1980-11-25 Hansen Joe C Sampling and identifying apparatus and method
US4318994A (en) * 1979-08-30 1982-03-09 Mcdonnell Douglas Corporation Enterobacteriaceae species biochemical test card

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1059592A (en) * 1976-07-26 1979-07-31 Harold J. Ostapovitch Box terminal for card edge receptacles in telecommunications systems and the like
FR2587722B1 (fr) * 1985-09-25 1988-05-20 Rhone Poulenc Agrochimie Procede de diagnostic des maladies fongiques des plantes et dispositif pour la mise en oeuvre de ce procede
JP2686199B2 (ja) * 1992-01-28 1997-12-08 矢崎総業株式会社 雌型端子金具

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3205151A (en) * 1962-04-17 1965-09-07 Hollister Inc Inoculation device and method
US3308039A (en) * 1966-02-15 1967-03-07 Allergan Pharma Disposable culturing device

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1418656A (fr) * 1964-12-23 1965-11-19 Hollister Inc Dispositif et procédé d'ensemencement de milieux d'essai

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3205151A (en) * 1962-04-17 1965-09-07 Hollister Inc Inoculation device and method
US3308039A (en) * 1966-02-15 1967-03-07 Allergan Pharma Disposable culturing device

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Enterotube Brochure; Roche Diagnostics; 1970 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3960658A (en) * 1974-09-23 1976-06-01 Centaur Chemical Co. Multi-media petri dish
US4235963A (en) * 1979-05-11 1980-11-25 Hansen Joe C Sampling and identifying apparatus and method
US4318994A (en) * 1979-08-30 1982-03-09 Mcdonnell Douglas Corporation Enterobacteriaceae species biochemical test card

Also Published As

Publication number Publication date
FR2185380A1 (xx) 1974-01-04
IT987390B (it) 1975-02-20
JPS4970487A (xx) 1974-07-08
NO141656B (no) 1980-01-07
ATA421373A (de) 1975-09-15
DK131581C (da) 1976-01-05
DE2322529A1 (de) 1973-12-13
AU456457B2 (en) 1974-12-19
DK131581B (da) 1975-08-04
CA995569A (en) 1976-08-24
IL42150A0 (en) 1973-06-29
GB1434092A (en) 1976-04-28
NO141656C (no) 1980-04-16
IL42150A (en) 1975-11-25
AU5526873A (en) 1974-11-07
NL156443B (nl) 1978-04-17
CH582748A5 (xx) 1976-12-15
AT330338B (de) 1976-06-25
SU539540A3 (ru) 1976-12-15
SE389127B (sv) 1976-10-25
BE799954A (fr) 1973-11-26
NL7306816A (xx) 1973-11-27
DE2322529B2 (de) 1975-06-05
FR2185380B1 (xx) 1976-06-11
DD106200A5 (xx) 1974-06-05
CS172256B2 (xx) 1976-12-29
JPS5422035B2 (xx) 1979-08-03

Similar Documents

Publication Publication Date Title
US2904474A (en) Process and means for carrying out bacteriological operations
KR101106453B1 (ko) 진단 테스트 장치 및 그 사용 방법
US5965453A (en) Test apparatus, system and method for the detection of test samples
EP0124193B1 (en) Method and device for bacterial testing
US5217875A (en) Method for detecting biological activities in a specimen and a device for implementing the method
AU771447B2 (en) Apparatus and method of testing a biological fluid
CA2125526A1 (en) Transport system for fluid analysis instrument
US4260687A (en) Diagnostic device
US3563859A (en) Disposable culture device
US4248830A (en) Device for microbiological testing
US3784448A (en) Diagnostic device
US3939044A (en) Anaerobic microbe transport assembly and method of using
CN110747118A (zh) 全自动全封闭多靶点核酸检测装置
CA1045530A (en) Analytical device and method
US3925163A (en) Diagnostic device
Cox et al. Selecting a miniaturized system for identification of Enterobacteriaceae
US3205151A (en) Inoculation device and method
WO1984003903A1 (en) Method and device for detecting microorganisms
EP0112077A1 (en) Diagnostic test probe
Schifman et al. Rapid automated identification of gram-negative bacilli from blood cultures with the AutoMicrobic system
CA1129324A (en) Device for detecting microorganisms
US4615979A (en) Diagnostic urine culture apparatus and method of using same
Bank et al. Screening urine specimen populations for normality using different dipsticks: evaluation of parameters influencing sensitivity and specificity
US4135981A (en) Device for recognition and differentiation of group d streptococci
Vejlsgaard et al. QUANTITATIVE BACTERIAL CULTURE OF URINE: II. Evaluation of 10 Different Screening Methods for the Diagnosis of Bacteriuria Compared with Results Obtained by the Dilution Technique