US3698822A - Disposable cuvettes for colorimetry - Google Patents

Disposable cuvettes for colorimetry Download PDF

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Publication number
US3698822A
US3698822A US100810A US3698822DA US3698822A US 3698822 A US3698822 A US 3698822A US 100810 A US100810 A US 100810A US 3698822D A US3698822D A US 3698822DA US 3698822 A US3698822 A US 3698822A
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United States
Prior art keywords
envelopes
compartment
sealed
specimen
testing
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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US100810A
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English (en)
Inventor
Michael L Polanyi
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American Optical Corp
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American Optical Corp
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Publication of US3698822A publication Critical patent/US3698822A/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/505—Containers for the purpose of retaining a material to be analysed, e.g. test tubes flexible containers not provided for above
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0364—Cuvette constructions flexible, compressible

Definitions

  • ABSTRACT I Colorimeter cuvettes in the form of sealed transparent flexible plastic envelopes containing or adapted to receive samples of substances to be colorimetrically analyzed. Means are provided for introducing sample I reagents into the envelopes. Mixing of the envelope contents may be effected by 'kneading prior to testing and the flexibility of the cuvettes allows for adjustment of optical path lengths therethrough.
  • the present invention overcomes the aforementioned and related drawbacks in colorimetric testing.
  • the present invention provides novel disposable cuvettes in the form of thin pliable sheet plastic envelopes containing samples requiring colorimetric testing or in the more usual case, containing a prepackaged sample diluent or suspension medium into which a sample to be tested may be injected immediately prior to testing thereof.
  • the invention contemplates the formation of a system of interconnected individually sealed sample containing or receiving envelopes in the form of an elongated plastic ribbon adapted to be cut transversely between any preselected pair of envelopes in the succession thereof so as to form a series test strip containing a preselected number of test sample envelopes. It is also contemplated that auxiliary individually sealed pockets or compartments be formed in the ribbon or test strip along one or both sides of each sample receiving envelope or intermediately of successive envelopes. A sealed but easily rupturable septum is provided between each auxiliary compartment and its adjacent sample containing or receiving envelope.
  • one or more sample reagents placed in one or more of the auxiliary compartments may be introduced into a test sample prior to or during colorimetric testing simply by squeezing together opposite sides of the auxiliary compartment whereby the septum will become ruptured and the sample reagent simultaneously forced into the sample containing envelope.
  • Mixing of the sample and reagent may be effected simply by kneading the pliable plastic envelope.
  • the path length of light directed through a sample therein may be established or adjusted according to the spacing provided between such parallel plates.
  • a completely sealed test sample may be examined colorimically any desired number of times with the same or different optical path lengths and with one or more reagents added thereto before or'during testing.
  • the sample upon completion of the testing of a particular sample, the sample need not be removed from its envelope (i.e. cuvette) for disposal but, instead, neatly and cleanly transported in the envelope and finally disposed of, envelope and all.
  • FIG. 1 is a plan view of one embodiment of the invention
  • FIG. 2 is a view similar to FIG. 1 'of a modification of the invention
  • FIG. 3 is a fragmentary cross-sectional view taken generally along line 3-3' of FIG. 2;
  • FIG. 4 is a fragmentary plan view of another modification ofthe invention. 1 I
  • FIG. 5 is a cross-sectional view taken generally along line 5-5 of FIG. 4;
  • FIG. 6 is a fragmentary plan view of still another modification of the invention.
  • FIG. 7 is a partially cross-sectioned diagrammatic illustration of colorimeter apparatus wherewith a use of disposable cuvettes according to principles of the present invention is illustrated.
  • FIGS. 1 and 2 there are illustrated embodiments of the present invention in its simplest form wherein disposable test sample cuvettes in the form of a system of envelopes 10 each containing or adapted to receive liquid and/or other types of sample substances or diluents are formed of thin pliable plastic sheet materials which are thermally welded or heat sealed together about the envelopes to form ribbon 12.
  • the superimposed sheets 14 and 16 of thin pliable plastic material which make up the ribbon are heat sealed or otherwise similarly fused to each other along their respective opposite edges and discriminately along paths 18 transversely of their longitudinal dimension whereby a continuous succession of test sample envelopes results.
  • Liquid test samples or sample diluents may be introduced into envelopes 10 during the formation of ribbon 12 or, as illustrated in FIG. 2, the sample or diluting medium and/or a reagent may be injected into a particular envelope with a hypodermic syringe 19 or the like.
  • a self-sealing plug 22 of elastomeric m'aterial such as a soft surgical rubber or the like is preferably heat sealed intothe structure of ribbon 12 between sheets 14 and 16 thereof in the manner illustrated in FIGS. 2 and 3, for example.
  • plugs 22 may be in the form of patches of self-sealing material fused or otherwise adhered to one or both sides of envelopes 10, preferably adjacent a corner thereof, or one or the other or both of the sheets 14 and 16 may be formed of a transparent elastomeric self-sealing material.
  • path length of light through substances being so tested may be critical to the test results. Shorter or longer path lengths may result in greater or lesser amounts respectively of light transmitted through a given one or a number of identical specimens whereby the test results may vary according to the thickness or depth of specimen being so transilluminated. Consideration of path lengths as a factor in test results may be eliminated according to the present inventive concept by positioning a selected sample containing env'elope 10 between rigid parallel transparent plates which are so constructed and arranged in a test apparatus 23 (see FIG. 7) as to be spaced one from the other at a given distance X at the time of testing.
  • One or the other or both of plates 20 may be arranged so as to be moveable toward and away from each other and thus freely receive an envelope 10. With an envelope 10 in this testing position, movement of plates 20 one toward the other to an extent controlled by stop or spacer means (not shown) therebetween for establishing the distance X provides a given path length through a test sample 24.
  • Envelopes 10 being formed of the aforesaid thin and pliable sheet materials and each being only partially filled with a test sample such as 24 will comply with adjustment of their thickness dimension by displacement of the liquid test sample 24 therein when positioned between plates 20 in the manner just described.
  • Apparatus 23 being illustrated schematically in FIG. 7 does not include a showing of simple mechanical expedients which may be employed for effecting the foresaid operation of plates 20 in receiving and positioning envelopes 10 for testing purposes. Such expedients would be well known to the artisan and are not intended to form a part of the present invention.
  • Colorimetric testing with apparatus such as is illustrated in FIG. 7, for example, is performed by projecting light from a source 26 through plates 20 and envelope 10 upon photodetector 28.
  • Circuit 30 having a source of electrical energy such as battery 32 and load resistor 34 connected in series with photodetector 28 also has leads 36 connected across resistor 34.
  • a conventional galvanometer or other recording device may be connected to leads 36 for providing an indication of electrical current variations produced in circuit by the effect of light impinging upon photodetector 28 which, in turn, provides an indication of the transmittance of sample 24.
  • envelopes 10 may be individually separated from ribbon 12 at anytime prior to, during or after testing in apparatus 23 or the ribbon may be kept intact.
  • FIG. 4 there is shown a modification of the invention wherein ribbon 12', being formed of thin and pliable plastic sheet materials 14' and 16' (FIG. 5), has main sample receiving or containing envelopes 10' which are generally similar to envelopes 10 of FIGS. 1 and 2. While means such as plugs 22 of FIGS. 2 and 3 or similar arrangements for injecting sample materials into packets 10 have not been shown, it should be understood that such may be incorporated in the structure of ribbon 12.
  • FIGS. 4 and 5 The embodiment of the invention represented by FIGS. 4 and 5 is directed more particularly to the provision of auxiliary pockets or sample diluent or reagent containing compartments 38.
  • Compartments 38 have reduced neck portions 40 leading toward similarly formed and contoured neck portions 42 extending laterally from envelopes 10'.
  • a rupturable septum 44 between compartments 38 and their respective envelopes 10 separate materials contained within each.
  • Septums 44 may be in the form of a thin superficial seal along a line between sheets 14' and 16' so that, by squeezing together opposite sides of compartments 38, pressure exerted against their contents will force the septums to rupture and thereby inject said contents into a respective envelope 10.
  • compartments 38 may be in a form of gas, liquid or powder reagents which are required to be added to particular samples within envelopes 10'. Forced mixing of such reagents with the samples may be accomplished by kneading of the pliable opposite sides of envelopes 10'.
  • auxiliary compartments such as 38 may vary in number with respect to the number of envelopes 10' and may be located at various points or positions relativeto envelopes 10 other than those illustrated in FIG. 4. For example, one other arrangement of envelopes 10' and compartments 38 is illustrated in FIG. 6 wherein compartments 38 are located successively intermediately of envelopes 10' of ribbon 12.
  • a ribbon formed of a P of superimposed Strips of said testing units each including a rupturable septum thin and pliable transparent plastic sheet material, said strips being sealed together along their corresponding opposite edges and further sealed normally sealing the auxiliary compartment from its corresponding-envelope whereby specimen reagents and other media sealed inside said compartto ether alon aths extendin across said ribbon 10 fr m one edgs the other to t orm a succession of ments may be 'l q Sald cofrespondmg enindividually circumferentially sealed envelopes i gi by F: and said strips further being sealed together along p 0 P e es 0 eac lines adjacent to at least one side of each of said of Said g p nent ly peripherally sealed sealed envelopes to form auxiliary compartments from commumcatlon adlacem other umts' along said ribbon, each envelope and at least one

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
US100810A 1969-03-19 1970-12-22 Disposable cuvettes for colorimetry Expired - Lifetime US3698822A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US80843769A 1969-03-19 1969-03-19
US10081070A 1970-12-22 1970-12-22

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US3698822A true US3698822A (en) 1972-10-17

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FR (1) FR2035058A1 (enExample)

Cited By (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3844662A (en) * 1971-07-13 1974-10-29 Froreich A Von Sedimentation instrument for body fluids and method of microscopic examination of the sediment
US3879106A (en) * 1973-04-11 1975-04-22 Pelam Inc Microscope slide cover slip
USRE30391E (en) * 1976-02-23 1980-09-02 Abbott Laboratories Chemical analysis cuvette
EP0056563A1 (fr) * 1981-01-19 1982-07-28 Société FINAMEX Finance Corporation Dispositif pour déterminer le groupe sanguin d'un individu
WO1986002545A1 (en) * 1984-10-29 1986-05-09 Bellhouse Medical Products Limited Blood bag
FR2590673A1 (fr) * 1985-11-05 1987-05-29 Bally Philippe Dispositif de test autonome miniaturise a usage unique
US4753531A (en) * 1983-11-29 1988-06-28 Fuji Photo Film Co., Ltd. Flat container type analytical instrument
EP0227796A4 (en) * 1985-06-18 1988-08-24 Baxter Diagnostics Inc COVERED CHAINS AND THEIR PRODUCTION PROCESS.
FR2612295A1 (fr) * 1987-03-11 1988-09-16 Abensour David Micro-analyseur automatique par colorimetrie ou bioluminescence
GB2206407A (en) * 1987-03-20 1989-01-05 Gerald David Whitlock Flexible sample sachet
US4863693A (en) * 1984-08-21 1989-09-05 E. I. Du Pont De Nemours And Company Analysis instrument having a blow molded reaction chamber
US5674457A (en) * 1995-04-21 1997-10-07 Hemocue Ab Capillary microcuvette
WO1997043619A1 (en) * 1996-05-13 1997-11-20 Dhc Analysis, Inc. Sampling device for spectrometric measurements
EP0706043A3 (en) * 1994-10-03 1998-05-20 Optical Solutions, Inc. Apparatus and method for measuring components in a bag
WO1999018422A1 (en) * 1997-10-03 1999-04-15 Hemocue Ab Production of microcuvettes
US6318191B1 (en) 1998-06-24 2001-11-20 Chen & Chen, Llc Fluid sample testing system
US20030049833A1 (en) * 1998-06-24 2003-03-13 Shuqi Chen Sample vessels
WO2006053588A1 (en) * 2004-11-17 2006-05-26 Agilent Technologies, Inc. Supply arrangement with supply reservoir element and fluidic device
US20080097285A1 (en) * 2006-10-20 2008-04-24 Cytyc Corporation Cell block cassette device
US7767447B2 (en) 2007-06-21 2010-08-03 Gen-Probe Incorporated Instruments and methods for exposing a receptacle to multiple thermal zones
US20100323919A1 (en) * 2004-06-07 2010-12-23 Iquum, Inc. Sample multiprocessing
US20120276532A1 (en) * 2000-12-29 2012-11-01 Chen & Chen, Llc Sample processing device for pretreatment and thermal cycling
US8936933B2 (en) 2003-02-05 2015-01-20 IQumm, Inc. Sample processing methods
US9005551B2 (en) 1998-06-24 2015-04-14 Roche Molecular Systems, Inc. Sample vessels
US20150298089A1 (en) * 1998-01-12 2015-10-22 Massachusetts Institute Of Technology Systems for Filling a Sample Array by Droplet Dragging
EP2290351B1 (de) * 2004-05-06 2016-12-21 CLONDIAG GmbH Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
EP3440449A4 (en) * 2016-04-08 2019-11-27 Veriphi Limited SAMPLE CONTAINER FOR SPECTROPHOTOMETRY

Cited By (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3844662A (en) * 1971-07-13 1974-10-29 Froreich A Von Sedimentation instrument for body fluids and method of microscopic examination of the sediment
US3879106A (en) * 1973-04-11 1975-04-22 Pelam Inc Microscope slide cover slip
USRE30391E (en) * 1976-02-23 1980-09-02 Abbott Laboratories Chemical analysis cuvette
EP0056563A1 (fr) * 1981-01-19 1982-07-28 Société FINAMEX Finance Corporation Dispositif pour déterminer le groupe sanguin d'un individu
WO1982002484A1 (fr) * 1981-01-19 1982-08-05 Zanchi Jean Dispositif pour determiner le groupe sanguin d'un individu
US4753531A (en) * 1983-11-29 1988-06-28 Fuji Photo Film Co., Ltd. Flat container type analytical instrument
US4863693A (en) * 1984-08-21 1989-09-05 E. I. Du Pont De Nemours And Company Analysis instrument having a blow molded reaction chamber
WO1986002545A1 (en) * 1984-10-29 1986-05-09 Bellhouse Medical Products Limited Blood bag
US4675019A (en) * 1984-10-29 1987-06-23 Bellhouse Medical Products Limited Blood monitoring system
US4758083A (en) * 1984-10-29 1988-07-19 Bellhouse Technology Limited Blood monitoring system
EP0227796A4 (en) * 1985-06-18 1988-08-24 Baxter Diagnostics Inc COVERED CHAINS AND THEIR PRODUCTION PROCESS.
FR2590673A1 (fr) * 1985-11-05 1987-05-29 Bally Philippe Dispositif de test autonome miniaturise a usage unique
FR2612295A1 (fr) * 1987-03-11 1988-09-16 Abensour David Micro-analyseur automatique par colorimetrie ou bioluminescence
GB2206407A (en) * 1987-03-20 1989-01-05 Gerald David Whitlock Flexible sample sachet
EP0706043A3 (en) * 1994-10-03 1998-05-20 Optical Solutions, Inc. Apparatus and method for measuring components in a bag
US5674457A (en) * 1995-04-21 1997-10-07 Hemocue Ab Capillary microcuvette
WO1997043619A1 (en) * 1996-05-13 1997-11-20 Dhc Analysis, Inc. Sampling device for spectrometric measurements
US6607701B1 (en) 1997-10-03 2003-08-19 Hemocue Ab Production of microcuvettes
WO1999018422A1 (en) * 1997-10-03 1999-04-15 Hemocue Ab Production of microcuvettes
US20150298089A1 (en) * 1998-01-12 2015-10-22 Massachusetts Institute Of Technology Systems for Filling a Sample Array by Droplet Dragging
US7833489B2 (en) * 1998-06-24 2010-11-16 Chen & Chen, Llc Fluid sample testing system
US7799521B2 (en) 1998-06-24 2010-09-21 Chen & Chen, Llc Thermal cycling
US10022722B2 (en) 1998-06-24 2018-07-17 Roche Molecular Systems, Inc. Sample vessels
US6748332B2 (en) 1998-06-24 2004-06-08 Chen & Chen, Llc Fluid sample testing system
US20080038813A1 (en) * 1998-06-24 2008-02-14 Shuqi Chen Sample vessels
US7337072B2 (en) 1998-06-24 2008-02-26 Chen & Chen, Llc Fluid sample testing system
US6318191B1 (en) 1998-06-24 2001-11-20 Chen & Chen, Llc Fluid sample testing system
US20080145275A1 (en) * 1998-06-24 2008-06-19 Chen & Chen, Llc Fluid sample testing system
US9005551B2 (en) 1998-06-24 2015-04-14 Roche Molecular Systems, Inc. Sample vessels
US20130040830A1 (en) * 1998-06-24 2013-02-14 Chen & Chen, Llc Fluid Sample Testing System
US20110064613A1 (en) * 1998-06-24 2011-03-17 Chen & Chen, Llc Fluid sample testing system
US20030049833A1 (en) * 1998-06-24 2003-03-13 Shuqi Chen Sample vessels
US9662652B2 (en) * 2000-12-29 2017-05-30 Chen & Chen, Llc Sample processing device for pretreatment and thermal cycling
US20120276532A1 (en) * 2000-12-29 2012-11-01 Chen & Chen, Llc Sample processing device for pretreatment and thermal cycling
US8936933B2 (en) 2003-02-05 2015-01-20 IQumm, Inc. Sample processing methods
US10443050B2 (en) 2003-02-05 2019-10-15 Roche Molecular Systems, Inc. Sample processing methods
US9708599B2 (en) 2003-02-05 2017-07-18 Roche Molecular Systems, Inc. Sample processing methods
EP2299257B1 (de) * 2004-05-06 2018-08-15 CLONDIAG GmbH Vorrichtung und verfahren zum nachweis von molekularen wechselwirkungen
EP3171155A1 (de) * 2004-05-06 2017-05-24 Clondiag GmbH Vorrichtung und verfahren zum nachweis von molekularen wechselwirkungen
EP2290351B1 (de) * 2004-05-06 2016-12-21 CLONDIAG GmbH Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
US20100323919A1 (en) * 2004-06-07 2010-12-23 Iquum, Inc. Sample multiprocessing
EP1771786A4 (en) * 2004-06-07 2011-05-25 Iquum Inc SAMPLE PROCESSING SIMULTANEOUS
US8414845B2 (en) 2004-06-07 2013-04-09 Iquum, Inc. Sample multiprocessing
WO2006053588A1 (en) * 2004-11-17 2006-05-26 Agilent Technologies, Inc. Supply arrangement with supply reservoir element and fluidic device
US20070263049A1 (en) * 2004-11-17 2007-11-15 Tobias Preckel Supply arrangement with supply reservoir element and microfluidic device
US7901946B2 (en) 2006-10-20 2011-03-08 Cytyc Corporation Cell block cassette device
US7666358B2 (en) * 2006-10-20 2010-02-23 Cytyc Corporation Cell block cassette device
US20100112629A1 (en) * 2006-10-20 2010-05-06 Cytyc Corporation Cell block cassette device
US20080097285A1 (en) * 2006-10-20 2008-04-24 Cytyc Corporation Cell block cassette device
US8765367B2 (en) 2007-06-21 2014-07-01 Gen-Probe Incorporated Methods and instruments for processing a sample in a multi-chambered receptacle
US9744506B2 (en) 2007-06-21 2017-08-29 Gen-Probe Incorporated Instruments for mixing the contents of a detection chamber
US8828654B2 (en) 2007-06-21 2014-09-09 Gen-Probe Incorporated Methods for manipulating liquid substances in multi-chambered receptacles
US8784745B2 (en) 2007-06-21 2014-07-22 Gen-Probe Incorporated Methods for manipulating liquid substances in multi-chambered receptacles
US8735055B2 (en) 2007-06-21 2014-05-27 Gen-Probe Incorporated Methods of concentrating an analyte
US8052929B2 (en) 2007-06-21 2011-11-08 Gen-Probe Incorporated Gravity-assisted mixing methods
US7780336B2 (en) 2007-06-21 2010-08-24 Gen-Probe Incorporated Instruments and methods for mixing the contents of a detection chamber
US8480976B2 (en) 2007-06-21 2013-07-09 Gen-Probe Incorporated Instruments and methods for mixing the contents of a detection chamber
US8048375B2 (en) 2007-06-21 2011-11-01 Gen-Probe Incorporated Gravity-assisted mixing methods
US7767447B2 (en) 2007-06-21 2010-08-03 Gen-Probe Incorporated Instruments and methods for exposing a receptacle to multiple thermal zones
US8491178B2 (en) 2007-06-21 2013-07-23 Gen-Probe Incorporated Instruments and methods for mixing the contents of a detection chamber
US11235294B2 (en) 2007-06-21 2022-02-01 Gen-Probe Incorporated System and method of using multi-chambered receptacles
US10688458B2 (en) 2007-06-21 2020-06-23 Gen-Probe Incorporated System and method of using multi-chambered receptacles
US10744469B2 (en) 2007-06-21 2020-08-18 Gen-Probe Incorporated Multi-chambered receptacles
US11235295B2 (en) 2007-06-21 2022-02-01 Gen-Probe Incorporated System and method of using multi-chambered receptacles
EP3440449A4 (en) * 2016-04-08 2019-11-27 Veriphi Limited SAMPLE CONTAINER FOR SPECTROPHOTOMETRY

Also Published As

Publication number Publication date
FR2035058A1 (enExample) 1970-12-18
DE2007405B2 (de) 1972-10-19
DE2007405A1 (de) 1970-10-08

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