US3623381A - Arrangements for treating and in particular analyzing a plurality of samples - Google Patents

Arrangements for treating and in particular analyzing a plurality of samples Download PDF

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Publication number
US3623381A
US3623381A US885662A US3623381DA US3623381A US 3623381 A US3623381 A US 3623381A US 885662 A US885662 A US 885662A US 3623381D A US3623381D A US 3623381DA US 3623381 A US3623381 A US 3623381A
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United States
Prior art keywords
distributor
samples
support
aforesaid
lever
Prior art date
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Expired - Lifetime
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US885662A
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English (en)
Inventor
Jean-Claude
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Choay SA
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Choay SA
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Publication date
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/24—Automatic injection systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/12—Dippers; Dredgers
    • G01N1/125—Dippers; Dredgers adapted for sampling molten metals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14—Suction devices, e.g. pumps; Ejector devices
    • G01N1/1409—Suction devices, e.g. pumps; Ejector devices adapted for sampling molten metals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T74/00—Machine element or mechanism
    • Y10T74/14—Rotary member or shaft indexing, e.g., tool or work turret
    • Y10T74/1418—Preselected indexed position
    • Y10T74/1424—Sequential
    • Y10T74/1453—Interlocked rotator and brake
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T74/00—Machine element or mechanism
    • Y10T74/15—Intermittent grip type mechanical movement
    • Y10T74/1526—Oscillation or reciprocation to intermittent unidirectional motion
    • Y10T74/1553—Lever actuator
    • Y10T74/1555—Rotary driven element

Definitions

  • This invention relates to arrangements for treating, and in particular analyzing, a plurality of samples, of the kind comprising, on the one hand, a device referred to hereinafter in a general manner by the term distributor, which is adapted to carry out at least part of the operations of treating or analyzing each sample at a fixed station, and, on the other hand, a support adapted to go through movements by discrete values or unitary movements enabling it to bring successive samples up to said station (it being understood that the word sample indicates any treatment unit such as a container, cup or cell or the like, the contents of which can be withdrawn, made up or treated in any other manner through the medium of the distributor); and it relates more particularly, in one of its preferred applications, to an arrangement designed to permit automatic analysis by chromatography, more particularly the automatic introduction of successive samples, for example amino acids, into a chromatography column filled with ion exchange resin.
  • Arrangements of this kind are already known, in particular automatic arrangements, in which the movement of the support is dependent on that of the distributor or vice versa.
  • the known arrangements are generally characterized by great complexity to enable the many conditions which must be complied with for satisfactory operation thereof to be constantly fulfilled.
  • the support must not go through more than one unitary movement each time it is operated by the distributor and must moreover be locked at least during the time when the appropriate operations are being carried out by the distributor on the sample which is at aforesaid fixed station at the time; on the other hand, the distributor must not be able to reach its working position or begin the execution of one of the aforesaid operations automatically if the support has not previously gone through a complete unitary movement, etc.
  • the object of the invention is therefore, above all, to render these arrangements such that they meet the various requirements in practice better than heretofore, in particular as regards both the simplicity and the reliability of control of the unitary movements of the support carrying the treatment units.
  • the arrangement according to the invention which comprises a distributor adapted to be driven with alternate movements between a rest position and a working position, in which latter it is capable of carrying out at least part of the operations on one of the samples at a fixed station, and a support on which the samples can be distributed at predetermined regular intervals and adapted to go through unitary movements enabling each of these samples to be brought up to the aforesaid fixed station in succession, is characterized by the fact that the support carries abutment elements in positions which are respectively in correspondence with those of the samples and the distributor carries a member adapted to rock in the direction of movement of the support from a position of equilibrium in which it is disposed, more particularly when the distributor is at rest, to a given off-set position, and to repel, at the cost of its inherent rocking action, one of the aforesaid abutment elements to produce a unitary movement of the plate every time the distributor is moved towards its working position, the aforesaid rocking action being sufficient for said member to be able to act
  • the distributor performs its reciprocating movements in a vertical direction and the support is constituted by a circular plate adapted to go through unitary angular movements about its axis, the samples being disposed on radii of said circular plate at respectively equal distances from the centre thereof, the driving elements being constituted by pegs projecting at the periphery of the plate and extending the aforesaid radii, the rocking member being constituted by a lever pivoting about a pin and having an inclined surface acting on the corresponding peg on the descent of the distributor.
  • the rocking member is advantageously carried by a part mounted on the distributor through the medium of resilient means, said part coming into abutment with a fixed part of the arrangement before the distributor has reached its working position, the distributor being then only able to complete its travel in opposition to the action of the aforesaid resilient means.
  • FIG. 1 of the drawing shows in perspective part of an arrangement for the automatic treatment of a plurality of samples
  • FIGS. 2 and 3 are enlarged elevational views of one of the parts of the arrangement of FIG. 1 in two positions which it is capable of adopting in the course of the operation thereof.
  • An arrangement in accordance with a preferred construction form of the invention and designed to permit automatic analyses by chromatography, more particularly the automatic introduction of successive samples, for example of amino acids, into chromatography columns filled with ion exchange resin, may be constituted more particularly as follows.
  • a sample support constituted, for example, by a circular plate 1 adapted to be driven with unitary angular movements about its axis and having a series of orifices 2 disposed, for example, in two concentric circular rows 2a and 21; on radii of this circular plate and defining seats for treatment units, for example cups or cells 4a and 4b containing the samples to be analysed, the unitary movements having the eifect of bringing each of these cups or cells up to a given fixed station in succession; and
  • the distributor which is preferably electropneumatically controlled, includes a jack 6, a compensating system (not shown) enabling the rate of descent of the jack to be regulated, and two parts 7a and 7b participating in the execution of the aforesaid operation on the samples contained in the cups 4a, 4b.
  • these parts 7a, 7b form elution heads made, for instance, of plastics material known commercially by the name of Kel-F; they are disposed at the end of the shaft of the jack and symmetrically with respect thereto, each of these parts being provided with an axial duct opening at the bottom and being provided with a coupling 8a or 812, for example of the type known by the name Swagelock, connecting the corresponding axial duct to an elution pump (not shown) through the medium of a polyethylene tube 9a or 9b.
  • the elution heads 7a, 7b are applied to the tops of the cups 401 and 45, respectively, the elution pumps aforesaid being then able to introduce measured amounts of eluent in manner known per se into the cups 4a, 41) through the medium of the tubes 9a, 9b and the elution heads 7a, 7b.
  • the cups 4a, 4b carried by the plate are pierced at their base, the samples of amino acids to be subjected to analysis being then adsorbed on a resin.
  • the cups 4a, 4b then communicate in manner known per se with two hollow parts (not shown), for example of Kel-F material, disposed below the circular plate and respectively communicating with the heads of two chromatography columns (not shown) and machined in such manner that the cups are applied intimately against them when the elution heads 7a, 7b bear on the tops of the cups 4a, 4b.
  • two hollow parts for example of Kel-F material
  • the successive cups 401, 4b can then be brought respectively up to the aforesaid fixed station by unitary angular rotary movements of the plate 1.
  • the mechanical drive of the circular plate 1 for causing it to go through the angular movements respectively equal in the example considered to an angle of 9 is effected through the medium of a part referred to as a whole by the reference 11, carried by the distributor 3 and adapted to engage with the support in such manner that it produces an angular movement of the plate when the distributor is brought from its rest position to its working position, the distributor being able to attain this latter position only if the plate has undergone a complete unitary rotation.
  • the circular plate has elements 12 preferably constituted by pegs mounted at the periphery of the plate and co-operating with a movable member 13 of the part 11 on the descent of the distributor into its working position, the engagement being accompanied by a displacement of the member 13 itself with respect to its inherent rest position, this displacement being sufficient to enable it to engage with the following peg when said member has found its position of equilibrium again and on a fresh descent of the distributor.
  • the movable member 12 aforesaid is advantageously constituted by a pivoting lever adapted to pivot about a pin 14 fast with the part 11 and having an inclined surface 16 which, when the distributor is in its rest position, is located perpendicularly above the peg 12 corresponding to that one of the treatment units which is then at the aforesaid fixed station.
  • the angular movement (in the direction of the arrow 17 which can be seen in FIG. 2) of the plate 1 (the relative position of which with respect to the part 11 is shown by chain-dotted lines in FIGS, 2 and 3) is obtained by the sliding action which is imposed on the peg 12 by the inclined surface 16 on the descent of the part 11 with the distributor 13 towards the working position of the latter.
  • the position of the pin 14 with respect to the inclined surface 16 of the lever 13 is such that this lever is impelled to rock after engagement from its inherent position of equilibrium (or position of rest on a surface 18 of the part 11) into a different ofi-set position (shown in FIG. 3) under the effect of the thrust exerted on the peg by the inclined surface of the lever, this rocking action being sufficient for said inclined surface to be located once more perpendicularly above the following peg 12a when the distributor and the part 11 have been brought back into their respective rest positions.
  • FIG. 2 shows the position of the lever 13 at the instant when it acts on the peg 12 which is perpendicularly below it in the course of the descent of the distributor 3
  • FIG. 3 shows the aforesaid different off-set position of the lever 13 (in abutment with a surface 19 of the part 11) at the end of the downward travel of the part 11.
  • the lever 13 Having returned to its position of equilibrium following the ascent of the distributor, the lever 13 will then be perpendicularly above the following peg 12a and when, in the course of the following descent of the distributor, it acts on this peg 12a, it is in the position shown in chain-dotted lines in FIG. 3.
  • the rocking of the lever 13 in the course of its action on a peg 12 is obtained automatically if the relative initial and final positions (shown in FIGS. 2 and 3, respectively) of the inclined surface 16 and a peg 12 are on each side of the vertical 21 (shown chain-dotted in FIG. 2) passing through the pivot 14 0f the lever.
  • the automatic return of the lever 13 to its inherent rest position is easily obtained if this rocking is accompanied by a relative raising of the centre of gravity of the lever 13, which can be achieved by giving the part 13a, which is adapted to come into contact either with the abutment surface 18 or with the abutment surface 19, a sufficient length from the pivot 14.
  • the arrest of the lateral movement of the peg 12 urged by the lever 13 is controlled by the arrest of the descending movement of the part 11 itself, it being possible to obtain this arrest by the abutment of its base 22 against a fixed part, for example the pedestal 23 (FIG. 1) supporting the plate 1.
  • the part 11 is normally held in a fixed relative position, in particular in a low position, with respect to the distributor 3 through the medium of resilient means such as a spring 24, the assembly consisting of the distributor 3 and the part 11 being arranged in such manner that the part 11 immobilizes the plate 1 before the distributor has itself completely finished its descending travel.
  • the completion of this descending travel, resulting in the application of the elution heads 70, 7b against the cups 4a, 4b, is then effected at the cost of a compression of the spring 24.
  • the part 11 always brings the elution heads and the cups into coincidence, without it therefore being necessary to effect periodic adjustments of the arrangement.
  • the final pressure of the distributor is applied only to the cups, so that the assembly can be subjected to considerable operating pressures, for example pressures of 70 kg./crn. in particular when the pneumatic system which has just been described is employed, as a result of which leakages need not be feared.
  • the arrangement according to the invention may therefore, for example, operate as follows.
  • the distributor then completes its travel, compressing the spring 24, until the instant when its elution heads 7a, 7b are applied against the cups 4a, 4b; the elution pump is then able to supply given volumes of eluents to the cups 4a, 4b through the tubes 9a, 9b and the elution heads 7a, 7b; at the end of the cycle of elution of the amino acids and after the arrest of the elution pumps another pulse is delivered to the control device of the electropneumatic jack and this then reverses the compressed air circuit, thus causing the ascent of the jack.
  • the lever 13 then returns to its rest position; and end-of-travel contact actuated when the distributor reaches is rest position produces a second electric pulse which is used to produce automatically a fresh descent of the distributor and the exchange of samples under the conditions which have been described hereinbefore.
  • a device for treating a plurality of samples comprising a distributor adapted to be driven with alternate movements between a rest position and a working position, in which latter it is capable of carrying out at least part of the operations on one of the samples at a fixed station, and a support on which the samples can be distributed at predetermined regular intervals, the support being adapted to be displaced by specific amounts to enable each of these samples to be brought up to the aforesaid fixed station in succession, wherein the improvement comprises abutment elements carried by the support in positions which correspond respectively with those of the samples and a member carried by the distributor disposed in a position of equilibrium relative to said distributor and out of contact with any of said abutment elements when said distributor is at rest, said member adaped to rock in the direction of movement of the support from a position of equilibrium in which it is disposed when the distributor is at rest, to a given off-set position, said member being further adapted when said distributor is moved towards its working position to meet and repel one of the aforesaid abut
  • the rocking member carried by the distributor comprises a lever pivotable about a pin and having a surface inclined with respect to the vertical, said inclined surface being located vertically above one of the abutment elements of the support when the distributor is in its rest position, said member going through a sufficient pivoting action about its pivot on the descent of the distributor and the driving of the support for said inclined surface to be located perpendicularly once more above the following abutment element of the support when the lever has been brought back into its position of equilibrium on the ascent of the distributor.
  • Device according to claim 3 which comprises abutment surfaces from the lever in both its position of equilibrium and its off-set position.
  • the support is constituted by a circular plate adapted to be displaced through unitary angular movements about its axis, the samples being disposed on radii of said circular plate at respectively equal distances from its centre, the abutment elements being constituted by pegs projecting radially from the periphery of the plate.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Hydrology & Water Resources (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
US885662A 1968-12-16 1969-12-16 Arrangements for treating and in particular analyzing a plurality of samples Expired - Lifetime US3623381A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR178476 1968-12-16
FR7135567A FR2154991A6 (enrdf_load_stackoverflow) 1968-12-16 1971-10-01

Publications (1)

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US3623381A true US3623381A (en) 1971-11-30

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US885662A Expired - Lifetime US3623381A (en) 1968-12-16 1969-12-16 Arrangements for treating and in particular analyzing a plurality of samples

Country Status (7)

Country Link
US (1) US3623381A (enrdf_load_stackoverflow)
BE (1) BE743049A (enrdf_load_stackoverflow)
CH (1) CH511171A (enrdf_load_stackoverflow)
DE (1) DE1962119A1 (enrdf_load_stackoverflow)
FR (2) FR1599279A (enrdf_load_stackoverflow)
GB (1) GB1292296A (enrdf_load_stackoverflow)
NL (1) NL6918869A (enrdf_load_stackoverflow)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS52141691A (en) * 1976-05-20 1977-11-26 Hata Iron Works Automatic hardness measuring device for compression molded body
US4271022A (en) * 1978-12-18 1981-06-02 Purdue Research Foundation Detection unit with solute detector and transport system
US5395521A (en) * 1991-05-31 1995-03-07 Board Of Regents, The University Of Texas System Automated column equilibration, column loading, column washing and column elution
EP0712000A3 (enrdf_load_stackoverflow) * 1994-11-10 1996-06-26 Ciba Corning Diagnostics Corp
US5823134A (en) * 1995-06-21 1998-10-20 Maasland N.V. Milk sampling device and method
WO2013067143A1 (en) * 2011-11-01 2013-05-10 Saudi Arabian Oil Company Multi-cuvette autosampler for photo-optical measurements

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2806159C3 (de) 1978-02-14 1980-09-18 Siemens Ag, 1000 Berlin Und 8000 Muenchen Tauchsieder
US4276258A (en) * 1980-01-28 1981-06-30 Coulter Electronics, Inc. Sample and stat feeding system and sample tray
DE3029440C2 (de) * 1980-08-02 1983-01-13 Ernst 4030 Ratingen Fooke Vorrichtung zum Waschen von Festkörpern

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS52141691A (en) * 1976-05-20 1977-11-26 Hata Iron Works Automatic hardness measuring device for compression molded body
US4271022A (en) * 1978-12-18 1981-06-02 Purdue Research Foundation Detection unit with solute detector and transport system
US5395521A (en) * 1991-05-31 1995-03-07 Board Of Regents, The University Of Texas System Automated column equilibration, column loading, column washing and column elution
EP0712000A3 (enrdf_load_stackoverflow) * 1994-11-10 1996-06-26 Ciba Corning Diagnostics Corp
US5599501A (en) * 1994-11-10 1997-02-04 Ciba Corning Diagnostics Corp. Incubation chamber
US5827478A (en) * 1994-11-10 1998-10-27 Chiron Diagnostics Corporation Incubation chamber
US5823134A (en) * 1995-06-21 1998-10-20 Maasland N.V. Milk sampling device and method
WO2013067143A1 (en) * 2011-11-01 2013-05-10 Saudi Arabian Oil Company Multi-cuvette autosampler for photo-optical measurements
US9028753B2 (en) 2011-11-01 2015-05-12 Saudi Arabian Oil Company Multi-cuvette autosampler for photo-optical measurements

Also Published As

Publication number Publication date
FR1599279A (enrdf_load_stackoverflow) 1970-07-15
GB1292296A (en) 1972-10-11
NL6918869A (enrdf_load_stackoverflow) 1970-06-18
FR2154991A6 (enrdf_load_stackoverflow) 1973-05-18
BE743049A (enrdf_load_stackoverflow) 1970-05-14
DE1962119A1 (de) 1970-06-18
DE1962119B2 (enrdf_load_stackoverflow) 1975-03-27
DE1962119C3 (enrdf_load_stackoverflow) 1975-11-20
CH511171A (fr) 1971-08-15

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