US3495490A - Microtome freezing attachment - Google Patents

Microtome freezing attachment Download PDF

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Publication number
US3495490A
US3495490A US3495490DA US3495490A US 3495490 A US3495490 A US 3495490A US 3495490D A US3495490D A US 3495490DA US 3495490 A US3495490 A US 3495490A
Authority
US
United States
Prior art keywords
specimen
knife
holder
attachment
microtome
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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English (en)
Inventor
Franz L Dollhopf
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandon Scientific Industries Ltd
Original Assignee
Shandon Scientific Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shandon Scientific Industries Ltd filed Critical Shandon Scientific Industries Ltd
Application granted granted Critical
Publication of US3495490A publication Critical patent/US3495490A/en
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Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T83/00—Cutting
    • Y10T83/283—With means to control or modify temperature of apparatus or work
    • Y10T83/293—Of tool

Definitions

  • sectioning movement of said other holder relative to said one holder is meant movement which with a specimen fitted to the specimen holder and a microtome knife fitted to the knife holder would result in the desired thin :sections -being cut from the specimen.
  • FIGURE 2 is a view in vertical section of the freezing attachment

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
US3495490D 1967-02-17 1968-02-19 Microtome freezing attachment Expired - Lifetime US3495490A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB774467A GB1170796A (en) 1967-02-17 1967-02-17 Improvements in or relating to Microtomes

Publications (1)

Publication Number Publication Date
US3495490A true US3495490A (en) 1970-02-17

Family

ID=9838893

Family Applications (1)

Application Number Title Priority Date Filing Date
US3495490D Expired - Lifetime US3495490A (en) 1967-02-17 1968-02-19 Microtome freezing attachment

Country Status (4)

Country Link
US (1) US3495490A (enrdf_load_stackoverflow)
DE (1) DE1622996C3 (enrdf_load_stackoverflow)
GB (1) GB1170796A (enrdf_load_stackoverflow)
SE (1) SE328426B (enrdf_load_stackoverflow)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3803958A (en) * 1969-06-02 1974-04-16 Moran H Fernandez Ultra thin sectioning with ultra sharp diamond edge at ultra low temperature
US3828571A (en) * 1972-09-06 1974-08-13 Reichert Optische Werke Ag Cover for microtome and ultramicrotome freezing chamber
US4012475A (en) * 1973-08-28 1977-03-15 Lkb-Produkter Ab Method for sectioning in a microtome at low temperature and trimming of the specimen to be sectioned
US4262567A (en) * 1979-10-01 1981-04-21 Bettin Elizabeth M Device for cooling microtome blade
WO1987002130A1 (en) * 1985-10-04 1987-04-09 Brian Laurence Jackson Improvements in cryostatic microtomes
US4918941A (en) * 1989-05-16 1990-04-24 Board Of Regents, The University Of Texas System Cryogenic ultramicrotome seal
US5533342A (en) * 1995-02-08 1996-07-09 Gordon; Abe Semi-automated cryostat
EP0833142A3 (de) * 1996-09-30 1999-03-10 MICROM LABORGERÄTE GmbH Kryostatmikrotom
US20070157633A1 (en) * 2006-01-10 2007-07-12 Honeywell International Inc. LN2 maintenance system

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2906153C2 (de) * 1979-02-17 1984-10-31 C. Reichert Optische Werke Ag, Wien Kühlkammer zur Aufnahme von zu bearbeitenden Objekten, insbesondere biologischen Objekten
DE3145505A1 (de) * 1981-11-17 1983-05-26 C. Reichert Optische Werke AG, 1170 Wien Transfereinrichtung zur entnahme von gefriergetrockneten schnitten aus der kuehlkammer eines gefriermikrotomes, insbesondere eines kryo-ultramikrotomes
AT390512B (de) * 1986-10-14 1990-05-25 Sitte Hellmuth Kuehlkammer zum herstellen von duennen schnitten bei reduzierter temperatur
DE4028806C2 (de) * 1990-09-11 1998-08-27 Leica Ag Mikrotom, insbesondere Ultramikrotom mit einer Kühlkammer
AT406309B (de) * 1998-09-01 2000-04-25 Leica Mikrosysteme Ag Vorrichtung zur regelung der temperaturen in einer kühlkammer für ein mikrotom

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3236133A (en) * 1963-05-06 1966-02-22 Whirlpool Co Microtome cooling and dehumidifying

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3236133A (en) * 1963-05-06 1966-02-22 Whirlpool Co Microtome cooling and dehumidifying

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3803958A (en) * 1969-06-02 1974-04-16 Moran H Fernandez Ultra thin sectioning with ultra sharp diamond edge at ultra low temperature
US3828571A (en) * 1972-09-06 1974-08-13 Reichert Optische Werke Ag Cover for microtome and ultramicrotome freezing chamber
US4012475A (en) * 1973-08-28 1977-03-15 Lkb-Produkter Ab Method for sectioning in a microtome at low temperature and trimming of the specimen to be sectioned
US4262567A (en) * 1979-10-01 1981-04-21 Bettin Elizabeth M Device for cooling microtome blade
WO1987002130A1 (en) * 1985-10-04 1987-04-09 Brian Laurence Jackson Improvements in cryostatic microtomes
US4918941A (en) * 1989-05-16 1990-04-24 Board Of Regents, The University Of Texas System Cryogenic ultramicrotome seal
US5533342A (en) * 1995-02-08 1996-07-09 Gordon; Abe Semi-automated cryostat
EP0833142A3 (de) * 1996-09-30 1999-03-10 MICROM LABORGERÄTE GmbH Kryostatmikrotom
US20070157633A1 (en) * 2006-01-10 2007-07-12 Honeywell International Inc. LN2 maintenance system

Also Published As

Publication number Publication date
DE1622996C3 (de) 1974-12-12
GB1170796A (en) 1969-11-19
DE1622996A1 (de) 1970-12-03
SE328426B (enrdf_load_stackoverflow) 1970-09-14
DE1622996B2 (de) 1974-05-09

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