US2360616A - Carrier for transferring tissues - Google Patents

Carrier for transferring tissues Download PDF

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Publication number
US2360616A
US2360616A US466709A US46670942A US2360616A US 2360616 A US2360616 A US 2360616A US 466709 A US466709 A US 466709A US 46670942 A US46670942 A US 46670942A US 2360616 A US2360616 A US 2360616A
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United States
Prior art keywords
tissues
carrier
reagent
base plate
transferring
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Expired - Lifetime
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US466709A
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Marlow W Olsen
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Individual
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Priority to US466709A priority Critical patent/US2360616A/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N2001/315—Basket-type carriers for tissues

Definitions

  • This invention relates to the art of histology and more particularly to a device for use in carrying fragile tissues and transferring them from one reagent to another.
  • the cellular structure of a-tissue be in as near its natural condition as possible when examined.
  • the tissues are usually treated with a series of chemical reagents before sectioning. Some of these reagents will kill, fix and harden the tissues; others act as dehydrating and clearing agents; and others infiltrate into the tissues and act as supporting structures while the tissues are being sectioned.
  • An object of this invention is to provide means whereby fragile tissues may be carried and transferred from one reagent to another without damage thereof.
  • a further object is to provide a simple method of constructing such a means.
  • Figure 1 is a plan view of the top of the carrier
  • Figure 2 is a cross section of line 22 in Figure 1.
  • the carrier has a metal base plate I having a plurality of perforations 2 therein, thus forming a screen.
  • a plurality of fiat metal strips 3, having perforations 4 are each bent into annular shape and are each secured at the edge to the base plate by soldering or in any other desirable manner. These together with the base plates, form a plurality of perforated individual cupshaped tissue-holding compartments 5.
  • the handle 6 is provided by a nut 1 centrally soldered or otherwise secured to the base plate, a, bolt 8 threadedly received in the nut 1 and a knob 9 secured to the head end of the bolt.
  • the base plate I and bands 3 are preferably made from Monel metal. If a corrodible metal be used, the carrier should be plated after its construction with a noncorrodible metal such as silver, chromium or cadmium.
  • the tissues to be dehydated, cleared and infiltrated are placed separately in the perforated individual compartments 5. Once the tissues are placed in their respective compartments, they need not be handled again until the time of imbedding.
  • the entire carrier is fitted into a petri dish which can be covered to prevent evaporation of the reagent being used.
  • the carrier is lifted from the first solution, drained and is then placed in a second petri dish containing the next reagent. Ths procedure greatly reduces the chances of damagin the tissues and makes it possible to quickly and easily transfer a large number of tissues with one operation from one reagent to the other.
  • a tissuecarrier comprising a noncorrodble base plate perforated to form a screen, a plurality of noncorrodible fiat perforated strips each bent into annular shape and secured at the edge to the base plate to form therewith a plurality of cup-shaped compartments for holding separate tissues, and a handle centrally attached on the base plate and extending above the cup-shaped compartments.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Description

Oct. 17, 1944. M. w. OLSEN CARRIER FOR TRANSFERRING TISSUES Filed NOV. 23, 1942 INVENTOR M. w. OLSEN a high powered microscope.
Patented Oct. 17, 1944 UNITED STATES PATENT OFFICE (Granted under the act of March 3, 1883, as
amended April 30, 1928; 370 O. G. 757) This application is made under the act of March 3, 1883, as amended by the act of April 30, 1928, and the invention herein described, if patented, may be manufactured and used by or for the Government of the United States of America for governmental purposes without the payment to me of any royalty thereon.
This invention relates to the art of histology and more particularly to a device for use in carrying fragile tissues and transferring them from one reagent to another.
The tissues of the majority of organisms are too thick and opaque to be studied as such under They must therefore be cut into thin and translucent sections before clear views of the minute individual structures can be obtained.
It is highly desirable that the cellular structure of a-tissue be in as near its natural condition as possible when examined. In order to prevent structural changes from occurring, the tissues are usually treated with a series of chemical reagents before sectioning. Some of these reagents will kill, fix and harden the tissues; others act as dehydrating and clearing agents; and others infiltrate into the tissues and act as supporting structures while the tissues are being sectioned.
It is common practice among histologists, while preparing tissues for sectioning, to transfer them from one reagent to another by means of a small forceps or spatula. Other workers follow the practice of placing the tissues, which are being prepared for sectioning, into small dishes or vials. The various reagents used are then added, removed, or replaced by other solutions, with the aid of a pipette. These methods can only be used safely when the tissue being studied is of such nature that it will not be injured by these operations. However, when the tissue is of a fragile nature extreme care must be exercised if the above methods be employed to prevent damage.
An object of this invention is to provide means whereby fragile tissues may be carried and transferred from one reagent to another without damage thereof. A further object is to provide a simple method of constructing such a means.
The invention is set forth in the following detailed description and attached drawing wherein like characters refer to like parts throughout the views and in which,
Figure 1 is a plan view of the top of the carrier, and
Figure 2 is a cross section of line 22 in Figure 1.
The carrier has a metal base plate I having a plurality of perforations 2 therein, thus forming a screen. A plurality of fiat metal strips 3, having perforations 4, are each bent into annular shape and are each secured at the edge to the base plate by soldering or in any other desirable manner. These together with the base plates, form a plurality of perforated individual cupshaped tissue-holding compartments 5. The handle 6 is provided by a nut 1 centrally soldered or otherwise secured to the base plate, a, bolt 8 threadedly received in the nut 1 and a knob 9 secured to the head end of the bolt. The base plate I and bands 3 are preferably made from Monel metal. If a corrodible metal be used, the carrier should be plated after its construction with a noncorrodible metal such as silver, chromium or cadmium.
In use, the tissues to be dehydated, cleared and infiltrated, are placed separately in the perforated individual compartments 5. Once the tissues are placed in their respective compartments, they need not be handled again until the time of imbedding. The entire carrier is fitted into a petri dish which can be covered to prevent evaporation of the reagent being used. To transfer the tissues to another reagent the carrier is lifted from the first solution, drained and is then placed in a second petri dish containing the next reagent. Ths procedure greatly reduces the chances of damagin the tissues and makes it possible to quickly and easily transfer a large number of tissues with one operation from one reagent to the other.
Having thus described my invention, I claim:
A tissuecarrier comprising a noncorrodble base plate perforated to form a screen, a plurality of noncorrodible fiat perforated strips each bent into annular shape and secured at the edge to the base plate to form therewith a plurality of cup-shaped compartments for holding separate tissues, and a handle centrally attached on the base plate and extending above the cup-shaped compartments.
MARLOW W. OLSEN.
US466709A 1942-11-23 1942-11-23 Carrier for transferring tissues Expired - Lifetime US2360616A (en)

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US466709A US2360616A (en) 1942-11-23 1942-11-23 Carrier for transferring tissues

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2837055A (en) * 1954-12-09 1958-06-03 Technicon International Ltd Tissue-holder receptacles
US3146906A (en) * 1962-02-08 1964-09-01 Rexall Drug Chemical Carrier tray
US3168100A (en) * 1962-12-07 1965-02-02 Alvido R Rich Contact lens dipper assembly
US3367529A (en) * 1965-11-29 1968-02-06 Welch John Mershon Bus box

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2837055A (en) * 1954-12-09 1958-06-03 Technicon International Ltd Tissue-holder receptacles
US3146906A (en) * 1962-02-08 1964-09-01 Rexall Drug Chemical Carrier tray
US3168100A (en) * 1962-12-07 1965-02-02 Alvido R Rich Contact lens dipper assembly
US3367529A (en) * 1965-11-29 1968-02-06 Welch John Mershon Bus box

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