US20220167630A1 - Phosphate-solubilzing fungal strains - Google Patents
Phosphate-solubilzing fungal strains Download PDFInfo
- Publication number
- US20220167630A1 US20220167630A1 US17/673,395 US202217673395A US2022167630A1 US 20220167630 A1 US20220167630 A1 US 20220167630A1 US 202217673395 A US202217673395 A US 202217673395A US 2022167630 A1 US2022167630 A1 US 2022167630A1
- Authority
- US
- United States
- Prior art keywords
- nrrl
- present disclosure
- composition
- bilaiae nrrl
- bilaiae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 230000002538 fungal effect Effects 0.000 title abstract description 55
- 239000000203 mixture Substances 0.000 claims abstract description 396
- 241000985541 Penicillium bilaiae Species 0.000 claims description 345
- 230000000813 microbial effect Effects 0.000 claims description 45
- 239000000463 material Substances 0.000 claims description 24
- 229920002774 Maltodextrin Polymers 0.000 claims description 23
- 230000001332 colony forming effect Effects 0.000 claims description 22
- 239000004021 humic acid Substances 0.000 claims description 22
- 229920000642 polymer Polymers 0.000 claims description 15
- 150000002016 disaccharides Chemical class 0.000 claims description 13
- 150000002772 monosaccharides Chemical class 0.000 claims description 13
- 150000005846 sugar alcohols Chemical class 0.000 claims description 13
- 239000002509 fulvic acid Substances 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 239000003415 peat Substances 0.000 claims description 5
- 229920001661 Chitosan Polymers 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 3
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 claims description 2
- PSZYNBSKGUBXEH-UHFFFAOYSA-N naphthalene-1-sulfonic acid Chemical class C1=CC=C2C(S(=O)(=O)O)=CC=CC2=C1 PSZYNBSKGUBXEH-UHFFFAOYSA-N 0.000 claims description 2
- 229920002101 Chitin Polymers 0.000 claims 1
- 229910019142 PO4 Inorganic materials 0.000 abstract description 66
- 239000010452 phosphate Substances 0.000 abstract description 59
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract description 58
- 238000000034 method Methods 0.000 abstract description 38
- 230000003381 solubilizing effect Effects 0.000 abstract description 4
- 239000002054 inoculum Substances 0.000 description 302
- 235000021317 phosphate Nutrition 0.000 description 63
- 244000005700 microbiome Species 0.000 description 59
- -1 but not limited to Substances 0.000 description 50
- 239000002609 medium Substances 0.000 description 40
- 241000193388 Bacillus thuringiensis Species 0.000 description 37
- 229940097012 bacillus thuringiensis Drugs 0.000 description 36
- 241000193744 Bacillus amyloliquefaciens Species 0.000 description 35
- 239000003795 chemical substances by application Substances 0.000 description 34
- 239000000843 powder Substances 0.000 description 29
- 238000003860 storage Methods 0.000 description 29
- 150000001875 compounds Chemical class 0.000 description 26
- 241000589174 Bradyrhizobium japonicum Species 0.000 description 24
- 235000014469 Bacillus subtilis Nutrition 0.000 description 23
- 244000063299 Bacillus subtilis Species 0.000 description 22
- 210000004027 cell Anatomy 0.000 description 21
- 239000000126 substance Substances 0.000 description 21
- 230000000087 stabilizing effect Effects 0.000 description 20
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 17
- 241001149558 Trichoderma virens Species 0.000 description 16
- 229910001868 water Inorganic materials 0.000 description 16
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 15
- 230000000694 effects Effects 0.000 description 15
- 239000007788 liquid Substances 0.000 description 14
- 241000194103 Bacillus pumilus Species 0.000 description 13
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 13
- 229940083542 sodium Drugs 0.000 description 13
- 229910052708 sodium Inorganic materials 0.000 description 13
- 239000011734 sodium Substances 0.000 description 13
- 239000002270 dispersing agent Substances 0.000 description 12
- LYCAIKOWRPUZTN-UHFFFAOYSA-N ethylene glycol Natural products OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 12
- 239000004094 surface-active agent Substances 0.000 description 12
- 241001000605 Semia Species 0.000 description 11
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 11
- 230000004083 survival effect Effects 0.000 description 11
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 10
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 10
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 10
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 10
- 230000003647 oxidation Effects 0.000 description 10
- 238000007254 oxidation reaction Methods 0.000 description 10
- 229910052700 potassium Inorganic materials 0.000 description 10
- 239000011591 potassium Substances 0.000 description 10
- 229960003975 potassium Drugs 0.000 description 10
- 238000012545 processing Methods 0.000 description 10
- 239000007787 solid Substances 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 9
- 239000001888 Peptone Substances 0.000 description 9
- 108010080698 Peptones Proteins 0.000 description 9
- 241000223260 Trichoderma harzianum Species 0.000 description 9
- 235000010323 ascorbic acid Nutrition 0.000 description 9
- 239000011668 ascorbic acid Substances 0.000 description 9
- 229960005070 ascorbic acid Drugs 0.000 description 9
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 9
- 230000012010 growth Effects 0.000 description 9
- 239000002736 nonionic surfactant Substances 0.000 description 9
- 235000019319 peptone Nutrition 0.000 description 9
- 229940066779 peptones Drugs 0.000 description 9
- 239000000049 pigment Substances 0.000 description 9
- 241000228143 Penicillium Species 0.000 description 8
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 8
- 239000003963 antioxidant agent Substances 0.000 description 8
- 235000006708 antioxidants Nutrition 0.000 description 8
- 239000000969 carrier Substances 0.000 description 8
- 238000005138 cryopreservation Methods 0.000 description 8
- 239000002274 desiccant Substances 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 8
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 description 8
- 239000000758 substrate Substances 0.000 description 8
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 7
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 7
- 241000223261 Trichoderma viride Species 0.000 description 7
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 7
- 229960002160 maltose Drugs 0.000 description 7
- DNIAPMSPPWPWGF-UHFFFAOYSA-N monopropylene glycol Natural products CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 6
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 description 6
- 241000233866 Fungi Species 0.000 description 6
- 235000019911 MALTRIN® Nutrition 0.000 description 6
- 241000223250 Metarhizium anisopliae Species 0.000 description 6
- 229940123973 Oxygen scavenger Drugs 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 229910052796 boron Inorganic materials 0.000 description 6
- 230000007423 decrease Effects 0.000 description 6
- 239000006185 dispersion Substances 0.000 description 6
- 235000011187 glycerol Nutrition 0.000 description 6
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 6
- 239000002773 nucleotide Substances 0.000 description 6
- 125000003729 nucleotide group Chemical group 0.000 description 6
- 231100000331 toxic Toxicity 0.000 description 6
- 230000002588 toxic effect Effects 0.000 description 6
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 5
- 241000193830 Bacillus <bacterium> Species 0.000 description 5
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 5
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 5
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 5
- 108010024636 Glutathione Proteins 0.000 description 5
- 229930195725 Mannitol Natural products 0.000 description 5
- 101000731004 Rattus norvegicus Membrane-associated progesterone receptor component 1 Proteins 0.000 description 5
- 241000319171 Talaromyces radicus Species 0.000 description 5
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 5
- 241000894120 Trichoderma atroviride Species 0.000 description 5
- 241001627203 Vema Species 0.000 description 5
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 5
- 239000000853 adhesive Substances 0.000 description 5
- 230000001070 adhesive effect Effects 0.000 description 5
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 235000014113 dietary fatty acids Nutrition 0.000 description 5
- 231100000673 dose–response relationship Toxicity 0.000 description 5
- 239000000194 fatty acid Substances 0.000 description 5
- 229930195729 fatty acid Natural products 0.000 description 5
- 229960003180 glutathione Drugs 0.000 description 5
- 239000000594 mannitol Substances 0.000 description 5
- 235000010355 mannitol Nutrition 0.000 description 5
- 229910052751 metal Inorganic materials 0.000 description 5
- 239000002184 metal Substances 0.000 description 5
- 239000003921 oil Substances 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 5
- 229920001223 polyethylene glycol Polymers 0.000 description 5
- 229920002451 polyvinyl alcohol Polymers 0.000 description 5
- 235000019422 polyvinyl alcohol Nutrition 0.000 description 5
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 5
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 5
- 235000013772 propylene glycol Nutrition 0.000 description 5
- 239000000600 sorbitol Substances 0.000 description 5
- 239000001993 wax Substances 0.000 description 5
- 239000000811 xylitol Substances 0.000 description 5
- 235000010447 xylitol Nutrition 0.000 description 5
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 5
- 229960002675 xylitol Drugs 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241000193747 Bacillus firmus Species 0.000 description 4
- 241001148114 Bradyrhizobium elkanii Species 0.000 description 4
- HEBKCHPVOIAQTA-QWWZWVQMSA-N D-arabinitol Chemical compound OC[C@@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-QWWZWVQMSA-N 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 4
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 4
- 241000244206 Nematoda Species 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 4
- 229920002319 Poly(methyl acrylate) Polymers 0.000 description 4
- 229920002873 Polyethylenimine Polymers 0.000 description 4
- 241000589194 Rhizobium leguminosarum Species 0.000 description 4
- 241000235504 Rhizophagus intraradices Species 0.000 description 4
- 241000222557 Rhizopogon Species 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 4
- 239000005849 Trichoderma asperellum (formerly T. harzianum) strains ICC012, T25 and TV1 Substances 0.000 description 4
- 241000290086 Yersinia entomophaga Species 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 4
- 230000000895 acaricidal effect Effects 0.000 description 4
- 239000003945 anionic surfactant Substances 0.000 description 4
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 4
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
- 150000002170 ethers Chemical class 0.000 description 4
- LZCLXQDLBQLTDK-UHFFFAOYSA-N ethyl 2-hydroxypropanoate Chemical compound CCOC(=O)C(C)O LZCLXQDLBQLTDK-UHFFFAOYSA-N 0.000 description 4
- 238000007710 freezing Methods 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 150000002334 glycols Chemical class 0.000 description 4
- 230000006872 improvement Effects 0.000 description 4
- AGBQKNBQESQNJD-UHFFFAOYSA-M lipoate Chemical compound [O-]C(=O)CCCCC1CCSS1 AGBQKNBQESQNJD-UHFFFAOYSA-M 0.000 description 4
- 235000019136 lipoic acid Nutrition 0.000 description 4
- 239000000314 lubricant Substances 0.000 description 4
- 235000019198 oils Nutrition 0.000 description 4
- 229920001542 oligosaccharide Polymers 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 230000008635 plant growth Effects 0.000 description 4
- 108091033319 polynucleotide Proteins 0.000 description 4
- 102000040430 polynucleotide Human genes 0.000 description 4
- 239000002157 polynucleotide Substances 0.000 description 4
- 229920000136 polysorbate Polymers 0.000 description 4
- 239000000377 silicon dioxide Substances 0.000 description 4
- 235000017557 sodium bicarbonate Nutrition 0.000 description 4
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- 239000000454 talc Substances 0.000 description 4
- 229910052623 talc Inorganic materials 0.000 description 4
- 229960002663 thioctic acid Drugs 0.000 description 4
- 239000000080 wetting agent Substances 0.000 description 4
- 229920001285 xanthan gum Polymers 0.000 description 4
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 description 3
- 101710171217 30S ribosomal protein S15 Proteins 0.000 description 3
- HBAQYPYDRFILMT-UHFFFAOYSA-N 8-[3-(1-cyclopropylpyrazol-4-yl)-1H-pyrazolo[4,3-d]pyrimidin-5-yl]-3-methyl-3,8-diazabicyclo[3.2.1]octan-2-one Chemical class C1(CC1)N1N=CC(=C1)C1=NNC2=C1N=C(N=C2)N1C2C(N(CC1CC2)C)=O HBAQYPYDRFILMT-UHFFFAOYSA-N 0.000 description 3
- 244000215068 Acacia senegal Species 0.000 description 3
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 3
- 241000589938 Azospirillum brasilense Species 0.000 description 3
- 241000194106 Bacillus mycoides Species 0.000 description 3
- 241001468265 Candidatus Phytoplasma Species 0.000 description 3
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 3
- 241000407877 Combretum Species 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- 108091092566 Extrachromosomal DNA Proteins 0.000 description 3
- 239000005715 Fructose Substances 0.000 description 3
- 229930091371 Fructose Natural products 0.000 description 3
- 229920002907 Guar gum Polymers 0.000 description 3
- 229920000084 Gum arabic Polymers 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- QAQJMLQRFWZOBN-LAUBAEHRSA-N L-ascorbyl-6-palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](O)[C@H]1OC(=O)C(O)=C1O QAQJMLQRFWZOBN-LAUBAEHRSA-N 0.000 description 3
- 239000011786 L-ascorbyl-6-palmitate Substances 0.000 description 3
- 229920001732 Lignosulfonate Polymers 0.000 description 3
- 239000005913 Maltodextrin Substances 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000589308 Methylobacterium extorquens Species 0.000 description 3
- 241000008865 Paraburkholderia denitrificans Species 0.000 description 3
- 241001025479 Penicillium bilaiae ATCC 20851 Species 0.000 description 3
- 241001300822 Pseudomonas jessenii Species 0.000 description 3
- 241000589166 Sinorhizobium fredii Species 0.000 description 3
- 241000223259 Trichoderma Species 0.000 description 3
- 241001460073 Trichoderma asperellum Species 0.000 description 3
- 241000894106 Trichoderma fertile Species 0.000 description 3
- 241000227728 Trichoderma hamatum Species 0.000 description 3
- 235000010489 acacia gum Nutrition 0.000 description 3
- 239000000205 acacia gum Substances 0.000 description 3
- 238000007792 addition Methods 0.000 description 3
- 125000000217 alkyl group Chemical group 0.000 description 3
- 239000003125 aqueous solvent Substances 0.000 description 3
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 3
- 235000010385 ascorbyl palmitate Nutrition 0.000 description 3
- QAOXGTOSYFOVIT-UHFFFAOYSA-N azanium 5-methylidenecyclopenta-1,3-diene-1-carboxylate Chemical compound [NH4+].[O-]C(=O)C1=CC=CC1=C QAOXGTOSYFOVIT-UHFFFAOYSA-N 0.000 description 3
- 239000012620 biological material Substances 0.000 description 3
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 3
- 239000001768 carboxy methyl cellulose Substances 0.000 description 3
- 239000004203 carnauba wax Substances 0.000 description 3
- 235000013869 carnauba wax Nutrition 0.000 description 3
- 235000021466 carotenoid Nutrition 0.000 description 3
- 150000001747 carotenoids Chemical class 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 150000004665 fatty acids Chemical class 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 229930003935 flavonoid Natural products 0.000 description 3
- 150000002215 flavonoids Chemical class 0.000 description 3
- 235000017173 flavonoids Nutrition 0.000 description 3
- 230000000855 fungicidal effect Effects 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- 235000010417 guar gum Nutrition 0.000 description 3
- 239000000665 guar gum Substances 0.000 description 3
- 229960002154 guar gum Drugs 0.000 description 3
- 229920000591 gum Polymers 0.000 description 3
- 229940035034 maltodextrin Drugs 0.000 description 3
- 239000002480 mineral oil Substances 0.000 description 3
- LWGJTAZLEJHCPA-UHFFFAOYSA-N n-(2-chloroethyl)-n-nitrosomorpholine-4-carboxamide Chemical compound ClCCN(N=O)C(=O)N1CCOCC1 LWGJTAZLEJHCPA-UHFFFAOYSA-N 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 235000021232 nutrient availability Nutrition 0.000 description 3
- 150000002482 oligosaccharides Chemical class 0.000 description 3
- 239000006174 pH buffer Substances 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 235000013930 proline Nutrition 0.000 description 3
- 150000003148 prolines Chemical class 0.000 description 3
- 230000001902 propagating effect Effects 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 238000005063 solubilization Methods 0.000 description 3
- 239000003549 soybean oil Substances 0.000 description 3
- 235000012424 soybean oil Nutrition 0.000 description 3
- GJJVAFUKOBZPCB-ZGRPYONQSA-N (r)-3,4-dihydro-2-methyl-2-(4,8,12-trimethyl-3,7,11-tridecatrienyl)-2h-1-benzopyran-6-ol Chemical class OC1=CC=C2OC(CC/C=C(C)/CC/C=C(C)/CCC=C(C)C)(C)CCC2=C1 GJJVAFUKOBZPCB-ZGRPYONQSA-N 0.000 description 2
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- MYRTYDVEIRVNKP-UHFFFAOYSA-N 1,2-Divinylbenzene Chemical compound C=CC1=CC=CC=C1C=C MYRTYDVEIRVNKP-UHFFFAOYSA-N 0.000 description 2
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 2
- QIGJYVCQYDKYDW-UHFFFAOYSA-N 3-O-alpha-D-mannopyranosyl-D-mannopyranose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(CO)OC(O)C1O QIGJYVCQYDKYDW-UHFFFAOYSA-N 0.000 description 2
- 244000020998 Acacia farnesiana Species 0.000 description 2
- 240000000073 Achillea millefolium Species 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 2
- 108010088751 Albumins Proteins 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 2
- 241000266341 Alternaria macrospora Species 0.000 description 2
- 241000471104 Alternaria oudemansii Species 0.000 description 2
- 239000004261 Ascorbyl stearate Substances 0.000 description 2
- LITUBCVUXPBCGA-WMZHIEFXSA-N Ascorbyl stearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@H](O)[C@H]1OC(=O)C(O)=C1O LITUBCVUXPBCGA-WMZHIEFXSA-N 0.000 description 2
- 241000320400 Azospirillum doebereinerae Species 0.000 description 2
- 241001470246 Bacillus amyloliquefaciens IT-45 Species 0.000 description 2
- 239000005731 Bacillus amyloliquefaciens MBI 600 Substances 0.000 description 2
- 241000193755 Bacillus cereus Species 0.000 description 2
- 239000005733 Bacillus pumilus QST 2808 Substances 0.000 description 2
- 241001104865 Bacillus subtilis GB03 Species 0.000 description 2
- 241000003114 Bacillus velezensis FZB42 Species 0.000 description 2
- 241000588882 Beijerinckia Species 0.000 description 2
- 241000959699 Bradyrhizobium lupini Species 0.000 description 2
- 241001453380 Burkholderia Species 0.000 description 2
- 241001135516 Burkholderia gladioli Species 0.000 description 2
- 241000134107 Burkholderia plantarii Species 0.000 description 2
- KAKZBPTYRLMSJV-UHFFFAOYSA-N Butadiene Chemical compound C=CC=C KAKZBPTYRLMSJV-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 2
- 241000317178 Claroideoglomus etunicatum Species 0.000 description 2
- 241000896542 Clonostachys rosea f. catenulata Species 0.000 description 2
- ACTIUHUUMQJHFO-UHFFFAOYSA-N Coenzym Q10 Natural products COC1=C(OC)C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UHFFFAOYSA-N 0.000 description 2
- 241001266001 Cordyceps confragosa Species 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 241000951565 Dolichospermum spiroides Species 0.000 description 2
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 description 2
- 239000004277 Ferrous carbonate Substances 0.000 description 2
- 240000000311 Freesia laxa Species 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- 241001311911 Funneliformis monosporum Species 0.000 description 2
- 241001123597 Funneliformis mosseae Species 0.000 description 2
- 241000235502 Gigaspora margarita Species 0.000 description 2
- 241000984804 Glomus aggregatum Species 0.000 description 2
- 244000286779 Hansenula anomala Species 0.000 description 2
- 108091023242 Internal transcribed spacer Proteins 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 2
- 241000188153 Isaria fumosorosea Species 0.000 description 2
- RRHGJUQNOFWUDK-UHFFFAOYSA-N Isoprene Chemical compound CC(=C)C=C RRHGJUQNOFWUDK-UHFFFAOYSA-N 0.000 description 2
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 description 2
- 241001489106 Laccaria laccata Species 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 description 2
- BAPJBEWLBFYGME-UHFFFAOYSA-N Methyl acrylate Chemical compound COC(=O)C=C BAPJBEWLBFYGME-UHFFFAOYSA-N 0.000 description 2
- 241001467578 Microbacterium Species 0.000 description 2
- 241000005783 Monographella albescens Species 0.000 description 2
- 241000192656 Nostoc Species 0.000 description 2
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 2
- 241000178961 Paenibacillus alvei Species 0.000 description 2
- 241001295760 Paraglomus Species 0.000 description 2
- 241001295759 Paraglomus brasilianum Species 0.000 description 2
- 241001668579 Pasteuria Species 0.000 description 2
- 235000019483 Peanut oil Nutrition 0.000 description 2
- 241001123663 Penicillium expansum Species 0.000 description 2
- 241000985530 Penicillium glabrum Species 0.000 description 2
- 241000228129 Penicillium janthinellum Species 0.000 description 2
- 241000364057 Peoria Species 0.000 description 2
- 241001272746 Phyllobacterium brassicacearum Species 0.000 description 2
- 241001503962 Pisolithus tinctorius Species 0.000 description 2
- 239000004260 Potassium ascorbate Substances 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- 241001465752 Purpureocillium lilacinum Species 0.000 description 2
- 241000714406 Rhizobium petrolearium Species 0.000 description 2
- 241000358085 Rhizophagus clarus Species 0.000 description 2
- 241001674300 Rhizopogon luteolus Species 0.000 description 2
- 241001466077 Salina Species 0.000 description 2
- 241001628499 Scleroderma cepa Species 0.000 description 2
- 241000959596 Scleroderma citrinum Species 0.000 description 2
- 241000357880 Septoglomus deserticola Species 0.000 description 2
- 241000043566 Sinorhizobium kummerowiae Species 0.000 description 2
- 229920002125 Sokalan® Polymers 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- ULUAUXLGCMPNKK-UHFFFAOYSA-N Sulfobutanedioic acid Chemical class OC(=O)CC(C(O)=O)S(O)(=O)=O ULUAUXLGCMPNKK-UHFFFAOYSA-N 0.000 description 2
- 235000019486 Sunflower oil Nutrition 0.000 description 2
- 239000005854 Trichoderma gamsii (formerly T. viride) strain ICC080 Substances 0.000 description 2
- 239000005855 Trichoderma harzianum strains T-22 and ITEM 908 Substances 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 2
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 2
- 239000000642 acaricide Substances 0.000 description 2
- 229960004308 acetylcysteine Drugs 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 235000010443 alginic acid Nutrition 0.000 description 2
- 229920000615 alginic acid Polymers 0.000 description 2
- 150000008055 alkyl aryl sulfonates Chemical class 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000019276 ascorbyl stearate Nutrition 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 229940005348 bacillus firmus Drugs 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 235000010376 calcium ascorbate Nutrition 0.000 description 2
- 229940047036 calcium ascorbate Drugs 0.000 description 2
- 239000011692 calcium ascorbate Substances 0.000 description 2
- BLORRZQTHNGFTI-ZZMNMWMASA-L calcium-L-ascorbate Chemical compound [Ca+2].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] BLORRZQTHNGFTI-ZZMNMWMASA-L 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000012876 carrier material Substances 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 2
- 239000003093 cationic surfactant Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 235000010980 cellulose Nutrition 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000004927 clay Substances 0.000 description 2
- 235000017471 coenzyme Q10 Nutrition 0.000 description 2
- ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 description 2
- 230000021615 conjugation Effects 0.000 description 2
- 229920001577 copolymer Polymers 0.000 description 2
- 239000006184 cosolvent Substances 0.000 description 2
- DIOQZVSQGTUSAI-UHFFFAOYSA-N decane Chemical compound CCCCCCCCCC DIOQZVSQGTUSAI-UHFFFAOYSA-N 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
- 235000019797 dipotassium phosphate Nutrition 0.000 description 2
- SNRUBQQJIBEYMU-UHFFFAOYSA-N dodecane Chemical compound CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 229940116333 ethyl lactate Drugs 0.000 description 2
- RAQDACVRFCEPDA-UHFFFAOYSA-L ferrous carbonate Chemical compound [Fe+2].[O-]C([O-])=O RAQDACVRFCEPDA-UHFFFAOYSA-L 0.000 description 2
- 235000019268 ferrous carbonate Nutrition 0.000 description 2
- 229960004652 ferrous carbonate Drugs 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 229930003944 flavone Natural products 0.000 description 2
- 150000002213 flavones Chemical class 0.000 description 2
- 235000011949 flavones Nutrition 0.000 description 2
- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 description 2
- 150000002216 flavonol derivatives Chemical class 0.000 description 2
- 235000011957 flavonols Nutrition 0.000 description 2
- 238000005189 flocculation Methods 0.000 description 2
- 230000016615 flocculation Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 229940095100 fulvic acid Drugs 0.000 description 2
- 229910021485 fumed silica Inorganic materials 0.000 description 2
- 239000000417 fungicide Substances 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 239000010439 graphite Substances 0.000 description 2
- 229910002804 graphite Inorganic materials 0.000 description 2
- 230000002363 herbicidal effect Effects 0.000 description 2
- 239000004009 herbicide Substances 0.000 description 2
- BXWNKGSJHAJOGX-UHFFFAOYSA-N hexadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCO BXWNKGSJHAJOGX-UHFFFAOYSA-N 0.000 description 2
- 150000002430 hydrocarbons Chemical class 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000000749 insecticidal effect Effects 0.000 description 2
- 239000002917 insecticide Substances 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 229910000015 iron(II) carbonate Inorganic materials 0.000 description 2
- 239000003077 lignite Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
- 229910001507 metal halide Inorganic materials 0.000 description 2
- 150000005309 metal halides Chemical class 0.000 description 2
- 229910044991 metal oxide Inorganic materials 0.000 description 2
- 150000004706 metal oxides Chemical class 0.000 description 2
- 229920000609 methyl cellulose Polymers 0.000 description 2
- 235000010981 methylcellulose Nutrition 0.000 description 2
- 239000010445 mica Substances 0.000 description 2
- 229910052618 mica group Inorganic materials 0.000 description 2
- 235000010446 mineral oil Nutrition 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 230000001069 nematicidal effect Effects 0.000 description 2
- 239000005645 nematicide Substances 0.000 description 2
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 2
- BKIMMITUMNQMOS-UHFFFAOYSA-N nonane Chemical compound CCCCCCCCC BKIMMITUMNQMOS-UHFFFAOYSA-N 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 239000004006 olive oil Substances 0.000 description 2
- 235000008390 olive oil Nutrition 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 2
- 239000000312 peanut oil Substances 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- YCOZIPAWZNQLMR-UHFFFAOYSA-N pentadecane Chemical compound CCCCCCCCCCCCCCC YCOZIPAWZNQLMR-UHFFFAOYSA-N 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 150000002989 phenols Chemical class 0.000 description 2
- 229920000747 poly(lactic acid) Polymers 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 229920001451 polypropylene glycol Polymers 0.000 description 2
- 229920002635 polyurethane Polymers 0.000 description 2
- 239000004814 polyurethane Substances 0.000 description 2
- 235000019275 potassium ascorbate Nutrition 0.000 description 2
- 229940017794 potassium ascorbate Drugs 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 159000000001 potassium salts Chemical class 0.000 description 2
- CONVKSGEGAVTMB-RXSVEWSESA-M potassium-L-ascorbate Chemical compound [K+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] CONVKSGEGAVTMB-RXSVEWSESA-M 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000008707 rearrangement Effects 0.000 description 2
- NPCOQXAVBJJZBQ-UHFFFAOYSA-N reduced coenzyme Q9 Natural products COC1=C(O)C(C)=C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)C(O)=C1OC NPCOQXAVBJJZBQ-UHFFFAOYSA-N 0.000 description 2
- 230000003362 replicative effect Effects 0.000 description 2
- 235000010378 sodium ascorbate Nutrition 0.000 description 2
- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium ascorbate Substances [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 description 2
- 229960005055 sodium ascorbate Drugs 0.000 description 2
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 2
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 2
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 230000007928 solubilization Effects 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 150000003871 sulfonates Chemical class 0.000 description 2
- 239000002600 sunflower oil Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 150000003573 thiols Chemical class 0.000 description 2
- 239000004408 titanium dioxide Substances 0.000 description 2
- 229930003799 tocopherol Natural products 0.000 description 2
- 239000011732 tocopherol Substances 0.000 description 2
- 125000002640 tocopherol group Chemical class 0.000 description 2
- 235000019149 tocopherols Nutrition 0.000 description 2
- 229930003802 tocotrienol Natural products 0.000 description 2
- 239000011731 tocotrienol Substances 0.000 description 2
- 229940068778 tocotrienols Drugs 0.000 description 2
- 235000019148 tocotrienols Nutrition 0.000 description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 2
- 230000026683 transduction Effects 0.000 description 2
- 238000010361 transduction Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 229940035936 ubiquinone Drugs 0.000 description 2
- 229940116269 uric acid Drugs 0.000 description 2
- 229920002554 vinyl polymer Polymers 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 239000002888 zwitterionic surfactant Substances 0.000 description 2
- HBDJFVFTHLOSDW-DNDLZOGFSA-N (2r,3r,4r,5r)-2,3,5,6-tetrahydroxy-4-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyhexanal;hydrate Chemical compound O.O=C[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HBDJFVFTHLOSDW-DNDLZOGFSA-N 0.000 description 1
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
- LUAHEUHBAZYUOI-KVXMBEGHSA-N (2s,3r,4r,5r)-4-[(2r,3r,4r,5s,6r)-5-[(2r,3r,4r,5s,6r)-3,4-dihydroxy-6-(hydroxymethyl)-5-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-3,4-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyhexane-1,2,3,5,6-pentol Chemical compound O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H](O)[C@@H](O)CO)[C@H](O)CO)O[C@H](CO)[C@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)[C@@H](CO)O1 LUAHEUHBAZYUOI-KVXMBEGHSA-N 0.000 description 1
- LGQKSQQRKHFMLI-SJYYZXOBSA-N (2s,3r,4s,5r)-2-[(3r,4r,5r,6r)-4,5,6-trihydroxyoxan-3-yl]oxyoxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)OC1 LGQKSQQRKHFMLI-SJYYZXOBSA-N 0.000 description 1
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 1
- GVJHHUAWPYXKBD-IEOSBIPESA-N (R)-alpha-Tocopherol Natural products OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 1
- JGTNAGYHADQMCM-UHFFFAOYSA-M 1,1,2,2,3,3,4,4,4-nonafluorobutane-1-sulfonate Chemical compound [O-]S(=O)(=O)C(F)(F)C(F)(F)C(F)(F)C(F)(F)F JGTNAGYHADQMCM-UHFFFAOYSA-M 0.000 description 1
- YFSUTJLHUFNCNZ-UHFFFAOYSA-M 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluorooctane-1-sulfonate Chemical compound [O-]S(=O)(=O)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)F YFSUTJLHUFNCNZ-UHFFFAOYSA-M 0.000 description 1
- TZCPCKNHXULUIY-RGULYWFUSA-N 1,2-distearoyl-sn-glycero-3-phosphoserine Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(=O)OC[C@H](N)C(O)=O)OC(=O)CCCCCCCCCCCCCCCCC TZCPCKNHXULUIY-RGULYWFUSA-N 0.000 description 1
- SERLAGPUMNYUCK-DCUALPFSSA-N 1-O-alpha-D-glucopyranosyl-D-mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O SERLAGPUMNYUCK-DCUALPFSSA-N 0.000 description 1
- OZCMOJQQLBXBKI-UHFFFAOYSA-N 1-ethenoxy-2-methylpropane Chemical compound CC(C)COC=C OZCMOJQQLBXBKI-UHFFFAOYSA-N 0.000 description 1
- UZKWTJUDCOPSNM-UHFFFAOYSA-N 1-ethenoxybutane Chemical compound CCCCOC=C UZKWTJUDCOPSNM-UHFFFAOYSA-N 0.000 description 1
- PZNPLUBHRSSFHT-RRHRGVEJSA-N 1-hexadecanoyl-2-octadecanoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCCCC(=O)O[C@@H](COP([O-])(=O)OCC[N+](C)(C)C)COC(=O)CCCCCCCCCCCCCCC PZNPLUBHRSSFHT-RRHRGVEJSA-N 0.000 description 1
- BPIUIOXAFBGMNB-UHFFFAOYSA-N 1-hexoxyhexane Chemical compound CCCCCCOCCCCCC BPIUIOXAFBGMNB-UHFFFAOYSA-N 0.000 description 1
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 241001673269 16SrIV (Coconut lethal yellows group) Species 0.000 description 1
- JAHNSTQSQJOJLO-UHFFFAOYSA-N 2-(3-fluorophenyl)-1h-imidazole Chemical compound FC1=CC=CC(C=2NC=CN=2)=C1 JAHNSTQSQJOJLO-UHFFFAOYSA-N 0.000 description 1
- OEPOKWHJYJXUGD-UHFFFAOYSA-N 2-(3-phenylmethoxyphenyl)-1,3-thiazole-4-carbaldehyde Chemical compound O=CC1=CSC(C=2C=C(OCC=3C=CC=CC=3)C=CC=2)=N1 OEPOKWHJYJXUGD-UHFFFAOYSA-N 0.000 description 1
- JKNCOURZONDCGV-UHFFFAOYSA-N 2-(dimethylamino)ethyl 2-methylprop-2-enoate Chemical compound CN(C)CCOC(=O)C(C)=C JKNCOURZONDCGV-UHFFFAOYSA-N 0.000 description 1
- LRYZPFWEZHSTHD-HEFFAWAOSA-O 2-[[(e,2s,3r)-2-formamido-3-hydroxyoctadec-4-enoxy]-hydroxyphosphoryl]oxyethyl-trimethylazanium Chemical class CCCCCCCCCCCCC\C=C\[C@@H](O)[C@@H](NC=O)COP(O)(=O)OCC[N+](C)(C)C LRYZPFWEZHSTHD-HEFFAWAOSA-O 0.000 description 1
- VHSHLMUCYSAUQU-UHFFFAOYSA-N 2-hydroxypropyl methacrylate Chemical compound CC(O)COC(=O)C(C)=C VHSHLMUCYSAUQU-UHFFFAOYSA-N 0.000 description 1
- JBVOQKNLGSOPNZ-UHFFFAOYSA-N 2-propan-2-ylbenzenesulfonic acid Chemical class CC(C)C1=CC=CC=C1S(O)(=O)=O JBVOQKNLGSOPNZ-UHFFFAOYSA-N 0.000 description 1
- UMCMPZBLKLEWAF-BCTGSCMUSA-N 3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulfonate Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 UMCMPZBLKLEWAF-BCTGSCMUSA-N 0.000 description 1
- IXOCGRPBILEGOX-UHFFFAOYSA-N 3-[3-(dodecanoylamino)propyl-dimethylazaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound CCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC(O)CS([O-])(=O)=O IXOCGRPBILEGOX-UHFFFAOYSA-N 0.000 description 1
- CDOUZKKFHVEKRI-UHFFFAOYSA-N 3-bromo-n-[(prop-2-enoylamino)methyl]propanamide Chemical compound BrCCC(=O)NCNC(=O)C=C CDOUZKKFHVEKRI-UHFFFAOYSA-N 0.000 description 1
- IICCLYANAQEHCI-UHFFFAOYSA-N 4,5,6,7-tetrachloro-3',6'-dihydroxy-2',4',5',7'-tetraiodospiro[2-benzofuran-3,9'-xanthene]-1-one Chemical compound O1C(=O)C(C(=C(Cl)C(Cl)=C2Cl)Cl)=C2C21C1=CC(I)=C(O)C(I)=C1OC1=C(I)C(O)=C(I)C=C21 IICCLYANAQEHCI-UHFFFAOYSA-N 0.000 description 1
- JLBJTVDPSNHSKJ-UHFFFAOYSA-N 4-Methylstyrene Chemical compound CC1=CC=C(C=C)C=C1 JLBJTVDPSNHSKJ-UHFFFAOYSA-N 0.000 description 1
- LGQKSQQRKHFMLI-UHFFFAOYSA-N 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(O)OC1 LGQKSQQRKHFMLI-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PZPXDAEZSA-N 4β-mannobiose Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-PZPXDAEZSA-N 0.000 description 1
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 229940046305 5-bromo-5-nitro-1,3-dioxane Drugs 0.000 description 1
- PVXPPJIGRGXGCY-DJHAAKORSA-N 6-O-alpha-D-glucopyranosyl-alpha-D-fructofuranose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@](O)(CO)O1 PVXPPJIGRGXGCY-DJHAAKORSA-N 0.000 description 1
- GJCOSYZMQJWQCA-UHFFFAOYSA-N 9H-xanthene Chemical compound C1=CC=C2CC3=CC=CC=C3OC2=C1 GJCOSYZMQJWQCA-UHFFFAOYSA-N 0.000 description 1
- 240000002460 Abroma augustum Species 0.000 description 1
- 241000287282 Acacia confluens Species 0.000 description 1
- 241000287343 Acacia implexa Species 0.000 description 1
- 241000589220 Acetobacter Species 0.000 description 1
- 241000589291 Acinetobacter Species 0.000 description 1
- 244000090532 Acorus terrestris Species 0.000 description 1
- 241001261165 Acropora monticulosa Species 0.000 description 1
- NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 240000005007 Actinomucor elegans Species 0.000 description 1
- 241000682855 Aegerita Species 0.000 description 1
- 241000886179 Aegilops neglecta Species 0.000 description 1
- 229910002012 Aerosil® Inorganic materials 0.000 description 1
- 240000007792 Aglaia odoratissima Species 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 241000321222 Agrobacterium larrymoorei Species 0.000 description 1
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 1
- 241001135511 Agrobacterium rubi Species 0.000 description 1
- 241000589176 Agrobacterium vitis Species 0.000 description 1
- 244000034249 Agropyron intermedium Species 0.000 description 1
- 241001626341 Akanthomyces Species 0.000 description 1
- 241001103808 Albifimbria verrucaria Species 0.000 description 1
- 241000588986 Alcaligenes Species 0.000 description 1
- 241000911802 Alishewanella tabrizica Species 0.000 description 1
- 244000244931 Allium canadense Species 0.000 description 1
- 241000016206 Allmaniopsis fruticulosa Species 0.000 description 1
- 241001073152 Aloe africana Species 0.000 description 1
- 244000191462 Aloe zebrina Species 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241000223600 Alternaria Species 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 241000210823 Amanita perpasta Species 0.000 description 1
- 241001430273 Aminobacter Species 0.000 description 1
- 241001646017 Aminobacter aganoensis Species 0.000 description 1
- 241001646016 Aminobacter aminovorans Species 0.000 description 1
- 241000979717 Aminobacter anthyllidis Species 0.000 description 1
- 241001628727 Aminobacter ciceronei Species 0.000 description 1
- 241000938042 Aminobacter lissarensis Species 0.000 description 1
- 241001646015 Aminobacter niigataensis Species 0.000 description 1
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- 244000100578 Amorphophallus variabilis Species 0.000 description 1
- 241000501812 Ampelomyces Species 0.000 description 1
- 241000501766 Ampelomyces quisqualis Species 0.000 description 1
- 241001612780 Amphisolenia schauinslandii Species 0.000 description 1
- 241000192542 Anabaena Species 0.000 description 1
- 241001554924 Anabaena aequalis Species 0.000 description 1
- 241001038551 Anabaena anomala Species 0.000 description 1
- 241000958677 Anabaena catenula Species 0.000 description 1
- 241000192537 Anabaena cylindrica Species 0.000 description 1
- 241000441560 Anabaena inaequalis Species 0.000 description 1
- 241000964129 Anabaena scheremetievi Species 0.000 description 1
- 241001555560 Anabaena sphaerica Species 0.000 description 1
- 241000648768 Anabaena subcylindrica Species 0.000 description 1
- 241000013735 Anabaena torulosa Species 0.000 description 1
- 241001370394 Anabaena verrucosa Species 0.000 description 1
- 241000716420 Apopellia megaspora Species 0.000 description 1
- 244000268610 Arctotis venusta Species 0.000 description 1
- 241000937828 Arenaria aggregata Species 0.000 description 1
- 241000924692 Aristolochia chilensis Species 0.000 description 1
- 241000032936 Aristolochia paucinervis Species 0.000 description 1
- 241000216674 Armillaria tabescens Species 0.000 description 1
- 241000584966 Artemisia alaskana Species 0.000 description 1
- 241000186063 Arthrobacter Species 0.000 description 1
- 241001524194 Arthrobacter agilis Species 0.000 description 1
- 241000722808 Arthrobotrys Species 0.000 description 1
- 241000297070 Arthrobotrys arthrobotryoides Species 0.000 description 1
- 241000215439 Arthrobotrys botryospora Species 0.000 description 1
- 241000215323 Arthrobotrys cladodes Species 0.000 description 1
- 241001262065 Arthrobotrys conoides Species 0.000 description 1
- 241000086836 Arthrobotrys dendroides Species 0.000 description 1
- 241000332450 Arthrobotrys ellipsospora Species 0.000 description 1
- 241001615685 Arthrobotrys entomopaga Species 0.000 description 1
- 241000215437 Arthrobotrys foliicola Species 0.000 description 1
- 241000392997 Arthrobotrys hertziana Species 0.000 description 1
- 241001464106 Arthrobotrys mangrovispora Species 0.000 description 1
- 241001212943 Arthrobotrys microscaphoides Species 0.000 description 1
- 241000961972 Arthrobotrys multisecundaria Species 0.000 description 1
- 241000215555 Arthrobotrys musiformis Species 0.000 description 1
- 241000722807 Arthrobotrys oligospora Species 0.000 description 1
- 241000263270 Arthrobotrys oviformis Species 0.000 description 1
- 241000215530 Arthrobotrys polycephala Species 0.000 description 1
- 241001272155 Arthrobotrys pseudoclavata Species 0.000 description 1
- 241000585736 Arthrobotrys pyriformis Species 0.000 description 1
- 241001278447 Arthrobotrys robusta Species 0.000 description 1
- 241000008500 Arthrobotrys scaphoides Species 0.000 description 1
- 241000139587 Arthrobotrys sclerohypha Species 0.000 description 1
- 241000215558 Arthrobotrys superba Species 0.000 description 1
- 241000215546 Arthrobotrys vermicola Species 0.000 description 1
- 241000037461 Arthrobotrys yunnanensis Species 0.000 description 1
- 241000445124 Arthrographis longispora Species 0.000 description 1
- 241000212115 Artomyces microsporus Species 0.000 description 1
- 241001443610 Aschersonia Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000228197 Aspergillus flavus Species 0.000 description 1
- 241000228230 Aspergillus parasiticus Species 0.000 description 1
- 241000724664 Asterochloris irregularis Species 0.000 description 1
- 241001555080 Aulosira Species 0.000 description 1
- 241000835972 Aulosira bohemensis Species 0.000 description 1
- 241001038553 Aulosira fertilissima Species 0.000 description 1
- 241000223651 Aureobasidium Species 0.000 description 1
- 241000223678 Aureobasidium pullulans Species 0.000 description 1
- 241000050634 Aureobasidium zeae Species 0.000 description 1
- 241000894008 Azorhizobium Species 0.000 description 1
- 241000894009 Azorhizobium caulinodans Species 0.000 description 1
- 241000404359 Azorhizobium oxalatiphilum Species 0.000 description 1
- 241000973036 Azorhizophilus paspali Species 0.000 description 1
- 241000589941 Azospirillum Species 0.000 description 1
- 241001643154 Azospirillum formosense Species 0.000 description 1
- 241000511673 Azospirillum largimobile Species 0.000 description 1
- 241000589939 Azospirillum lipoferum Species 0.000 description 1
- 241000030681 Azospirillum melinis Species 0.000 description 1
- 241000274120 Azospirillum picis Species 0.000 description 1
- 241001018114 Azospirillum rugosum Species 0.000 description 1
- 241001108772 Azospirillum thiophilum Species 0.000 description 1
- 241000589151 Azotobacter Species 0.000 description 1
- 241000408716 Azotobacter armeniacus Species 0.000 description 1
- 241000681683 Azotobacter beijerinckii Species 0.000 description 1
- 241000589152 Azotobacter chroococcum Species 0.000 description 1
- 241000408717 Azotobacter nigricans Species 0.000 description 1
- 241000973041 Azotobacter salinestris Species 0.000 description 1
- 241000738599 Azotobacter tropicalis Species 0.000 description 1
- 241000589149 Azotobacter vinelandii Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000223679 Beauveria Species 0.000 description 1
- 241000751139 Beauveria bassiana Species 0.000 description 1
- 241000427199 Bosea <angiosperm> Species 0.000 description 1
- 241001631208 Bosea eneae Species 0.000 description 1
- 241001019268 Bosea lathyri Species 0.000 description 1
- 241001631288 Bosea massiliensis Species 0.000 description 1
- 241000610438 Bosea minatitlanensis Species 0.000 description 1
- 241001019247 Bosea robiniae Species 0.000 description 1
- 241000519601 Bosea thiooxidans Species 0.000 description 1
- 241001631289 Bosea vestrisii Species 0.000 description 1
- 241000589173 Bradyrhizobium Species 0.000 description 1
- 241001342068 Bradyrhizobium arachidis Species 0.000 description 1
- 241001378169 Bradyrhizobium canariense Species 0.000 description 1
- 241000714136 Bradyrhizobium cytisi Species 0.000 description 1
- 241000795894 Bradyrhizobium daqingense Species 0.000 description 1
- 241000845990 Bradyrhizobium diazoefficiens Species 0.000 description 1
- 241001592599 Bradyrhizobium diazoefficiens SEMIA 5080 Species 0.000 description 1
- 241000274790 Bradyrhizobium diazoefficiens USDA 110 Species 0.000 description 1
- 241000528752 Bradyrhizobium ganzhouense Species 0.000 description 1
- 241000206124 Bradyrhizobium icense Species 0.000 description 1
- 241000390783 Bradyrhizobium ingae Species 0.000 description 1
- 241000407956 Bradyrhizobium iriomotense Species 0.000 description 1
- 241001551803 Bradyrhizobium japonicum SEMIA 5079 Species 0.000 description 1
- 241001036481 Bradyrhizobium japonicum USDA 122 Species 0.000 description 1
- 241000303569 Bradyrhizobium japonicum USDA 123 Species 0.000 description 1
- 241001005637 Bradyrhizobium japonicum USDA 6 Species 0.000 description 1
- 241000757064 Bradyrhizobium jicamae Species 0.000 description 1
- 241000462823 Bradyrhizobium lablabi Species 0.000 description 1
- 241000159535 Bradyrhizobium liaoningense Species 0.000 description 1
- 241000496858 Bradyrhizobium manausense Species 0.000 description 1
- 241000390760 Bradyrhizobium neotropicale Species 0.000 description 1
- 241000178956 Bradyrhizobium oligotrophicum Species 0.000 description 1
- 241000239999 Bradyrhizobium ottawaense Species 0.000 description 1
- 241000757063 Bradyrhizobium pachyrhizi Species 0.000 description 1
- 241001411663 Bradyrhizobium paxllaeri Species 0.000 description 1
- 241000606194 Bradyrhizobium retamae Species 0.000 description 1
- 241000714149 Bradyrhizobium rifense Species 0.000 description 1
- 241000512875 Bradyrhizobium valentinum Species 0.000 description 1
- 241001545610 Bradyrhizobium yuanmingense Species 0.000 description 1
- 241000555281 Brevibacillus Species 0.000 description 1
- 229910000906 Bronze Inorganic materials 0.000 description 1
- 241001646647 Burkholderia ambifaria Species 0.000 description 1
- 241000790236 Burkholderia anthina Species 0.000 description 1
- 241000283590 Burkholderia arboris Species 0.000 description 1
- 241000589513 Burkholderia cepacia Species 0.000 description 1
- 241000274234 Burkholderia contaminans Species 0.000 description 1
- 241000283588 Burkholderia diffusa Species 0.000 description 1
- 241001646389 Burkholderia dolosa Species 0.000 description 1
- 241000589638 Burkholderia glumae Species 0.000 description 1
- 241000202968 Burkholderia lata Species 0.000 description 1
- 241000274232 Burkholderia latens Species 0.000 description 1
- 241000722910 Burkholderia mallei Species 0.000 description 1
- 241000283585 Burkholderia metallica Species 0.000 description 1
- 241000020731 Burkholderia multivorans Species 0.000 description 1
- 241000696607 Burkholderia oklahomensis Species 0.000 description 1
- 241001136175 Burkholderia pseudomallei Species 0.000 description 1
- 241000978368 Burkholderia pseudomultivorans Species 0.000 description 1
- 241000283591 Burkholderia seminalis Species 0.000 description 1
- 241000241702 Burkholderia singaporensis Species 0.000 description 1
- 241000371422 Burkholderia stabilis Species 0.000 description 1
- 241000581608 Burkholderia thailandensis Species 0.000 description 1
- 241001459282 Burkholderia ubonensis Species 0.000 description 1
- 241000866606 Burkholderia vietnamiensis Species 0.000 description 1
- 241000003078 Caballeronia choica Species 0.000 description 1
- 241000866631 Caballeronia glathei Species 0.000 description 1
- 241000753810 Caballeronia grimmiae Species 0.000 description 1
- 241000003076 Caballeronia humi Species 0.000 description 1
- 241000003077 Caballeronia telluris Species 0.000 description 1
- 241000039258 Caballeronia terrestris Species 0.000 description 1
- 241000393956 Caballeronia udeis Species 0.000 description 1
- 241000338144 Caballeronia zhejiangensis Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000577959 Calonectria Species 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241000771897 Candidatus Burkholderia crenata Species 0.000 description 1
- 241000773394 Candidatus Burkholderia mamillata Species 0.000 description 1
- 241000988054 Candidatus Burkholderia verschuerenii Species 0.000 description 1
- 241000773385 Candidatus Burkholderia virens Species 0.000 description 1
- 241000988050 Candidatus Paraburkholderia calva Species 0.000 description 1
- 241000462044 Candidatus Paraburkholderia hispidae Species 0.000 description 1
- 241001517078 Candidatus Paraburkholderia kirkii Species 0.000 description 1
- 241000988048 Candidatus Paraburkholderia nigropunctata Species 0.000 description 1
- 241000462042 Candidatus Paraburkholderia rigidae Species 0.000 description 1
- 241000462051 Candidatus Paraburkholderia schumannianae Species 0.000 description 1
- 241001226190 Candidatus Phytoplasma allocasuarinae Species 0.000 description 1
- 241000767079 Candidatus Phytoplasma americanum Species 0.000 description 1
- 241001673276 Candidatus Phytoplasma asteris Species 0.000 description 1
- 241001047513 Candidatus Phytoplasma aurantifolia Species 0.000 description 1
- 241000611257 Candidatus Phytoplasma australiense Species 0.000 description 1
- 241000652417 Candidatus Phytoplasma balanitae Species 0.000 description 1
- 241001464372 Candidatus Phytoplasma brasiliense Species 0.000 description 1
- 241001431260 Candidatus Phytoplasma caricae Species 0.000 description 1
- 241001644148 Candidatus Phytoplasma castaneae Species 0.000 description 1
- 241001252373 Candidatus Phytoplasma convolvuli Species 0.000 description 1
- 241001119058 Candidatus Phytoplasma costaricanum Species 0.000 description 1
- 241001252370 Candidatus Phytoplasma cynodontis Species 0.000 description 1
- 241000401029 Candidatus Phytoplasma fragariae Species 0.000 description 1
- 241001495406 Candidatus Phytoplasma fraxini Species 0.000 description 1
- 241001149705 Candidatus Phytoplasma graminis Species 0.000 description 1
- 241001464373 Candidatus Phytoplasma japonicum Species 0.000 description 1
- 241001495380 Candidatus Phytoplasma luffae Species 0.000 description 1
- 241000857664 Candidatus Phytoplasma lycopersici Species 0.000 description 1
- 241001522082 Candidatus Phytoplasma mali Species 0.000 description 1
- 241001179025 Candidatus Phytoplasma omanense Species 0.000 description 1
- 241001182423 Candidatus Phytoplasma oryzae Species 0.000 description 1
- 241000405852 Candidatus Phytoplasma palmicola Species 0.000 description 1
- 241001520063 Candidatus Phytoplasma phoenicium Species 0.000 description 1
- 241000110058 Candidatus Phytoplasma pini Species 0.000 description 1
- 241001594368 Candidatus Phytoplasma pruni Species 0.000 description 1
- 241000217221 Candidatus Phytoplasma prunorum Species 0.000 description 1
- 241000217224 Candidatus Phytoplasma pyri Species 0.000 description 1
- 241001034275 Candidatus Phytoplasma rhamni Species 0.000 description 1
- 241000949204 Candidatus Phytoplasma rubi Species 0.000 description 1
- 241000985905 Candidatus Phytoplasma solani Species 0.000 description 1
- 241000582784 Candidatus Phytoplasma spartii Species 0.000 description 1
- 241000875810 Candidatus Phytoplasma sudamericanum Species 0.000 description 1
- 241001219318 Candidatus Phytoplasma tamaricis Species 0.000 description 1
- 241001495375 Candidatus Phytoplasma trifolii Species 0.000 description 1
- 241001495383 Candidatus Phytoplasma ulmi Species 0.000 description 1
- 241000900200 Candidatus Phytoplasma vitis Species 0.000 description 1
- 241000947250 Candidatus Phytoplasma ziziphi Species 0.000 description 1
- 240000000533 Capsicum pubescens Species 0.000 description 1
- 240000001817 Cereus hexagonus Species 0.000 description 1
- 241000588881 Chromobacterium Species 0.000 description 1
- 241001332334 Chromobacterium subtsugae Species 0.000 description 1
- 240000004439 Cistus albidus Species 0.000 description 1
- 241000186650 Clavibacter Species 0.000 description 1
- 241000142531 Clonostachys Species 0.000 description 1
- 241000193403 Clostridium Species 0.000 description 1
- 241000659049 Coelomycidium Species 0.000 description 1
- 241000222199 Colletotrichum Species 0.000 description 1
- 241001133184 Colletotrichum agaves Species 0.000 description 1
- 241000589519 Comamonas Species 0.000 description 1
- 241001480517 Conidiobolus Species 0.000 description 1
- 241001312183 Coniothyrium Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000190633 Cordyceps Species 0.000 description 1
- 241000186216 Corynebacterium Species 0.000 description 1
- 241001362614 Crassa Species 0.000 description 1
- 241000221755 Cryphonectria Species 0.000 description 1
- 241000221199 Cryptococcus <basidiomycete yeast> Species 0.000 description 1
- 241001337994 Cryptococcus <scale insect> Species 0.000 description 1
- 241001136984 Cryptophlebia Species 0.000 description 1
- 241001626319 Culicinomyces Species 0.000 description 1
- 241001528480 Cupriavidus Species 0.000 description 1
- 241000615080 Cupriavidus alkaliphilus Species 0.000 description 1
- 241000960359 Cupriavidus basilensis Species 0.000 description 1
- 241001640455 Cupriavidus campinensis Species 0.000 description 1
- 241001634906 Cupriavidus gilardii Species 0.000 description 1
- 241001641098 Cupriavidus laharis Species 0.000 description 1
- 241000252867 Cupriavidus metallidurans Species 0.000 description 1
- 241000238950 Cupriavidus numazuensis Species 0.000 description 1
- 241000382839 Cupriavidus oxalaticus Species 0.000 description 1
- 241000278502 Cupriavidus pampae Species 0.000 description 1
- 241001635226 Cupriavidus pauculus Species 0.000 description 1
- 241001050521 Cupriavidus pinatubonensis Species 0.000 description 1
- 241001470441 Cupriavidus respiraculi Species 0.000 description 1
- 241000203813 Curtobacterium Species 0.000 description 1
- 241001464430 Cyanobacterium Species 0.000 description 1
- 241001634817 Cydia Species 0.000 description 1
- 241001635274 Cydia pomonella Species 0.000 description 1
- 102000000634 Cytochrome c oxidase subunit IV Human genes 0.000 description 1
- 108090000365 Cytochrome-c oxidases Proteins 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- WQZGKKKJIJFFOK-CBPJZXOFSA-N D-Gulose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O WQZGKKKJIJFFOK-CBPJZXOFSA-N 0.000 description 1
- WQZGKKKJIJFFOK-WHZQZERISA-N D-aldose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-WHZQZERISA-N 0.000 description 1
- WQZGKKKJIJFFOK-IVMDWMLBSA-N D-allopyranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@H](O)[C@@H]1O WQZGKKKJIJFFOK-IVMDWMLBSA-N 0.000 description 1
- OXQKEKGBFMQTML-UHFFFAOYSA-N D-glycero-D-gluco-heptitol Natural products OCC(O)C(O)C(O)C(O)C(O)CO OXQKEKGBFMQTML-UHFFFAOYSA-N 0.000 description 1
- FBPFZTCFMRRESA-ZXXMMSQZSA-N D-iditol Chemical compound OC[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-ZXXMMSQZSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- UNXHWFMMPAWVPI-QWWZWVQMSA-N D-threitol Chemical compound OC[C@@H](O)[C@H](O)CO UNXHWFMMPAWVPI-QWWZWVQMSA-N 0.000 description 1
- YTBSYETUWUMLBZ-QWWZWVQMSA-N D-threose Chemical compound OC[C@@H](O)[C@H](O)C=O YTBSYETUWUMLBZ-QWWZWVQMSA-N 0.000 description 1
- SQNRKWHRVIAKLP-UHFFFAOYSA-N D-xylobiose Natural products O=CC(O)C(O)C(CO)OC1OCC(O)C(O)C1O SQNRKWHRVIAKLP-UHFFFAOYSA-N 0.000 description 1
- 241000215396 Dactylaria Species 0.000 description 1
- 241001210847 Dactylella cylindrospora Species 0.000 description 1
- 241001005786 Dactylella shizishanna Species 0.000 description 1
- 241000215369 Dactylellina cionopaga Species 0.000 description 1
- 244000057399 Dalea candida Species 0.000 description 1
- 241001600129 Delftia Species 0.000 description 1
- 241001600125 Delftia acidovorans Species 0.000 description 1
- 241000192652 Desmonostoc muscorum Species 0.000 description 1
- 241000605716 Desulfovibrio Species 0.000 description 1
- 241000205646 Devosia Species 0.000 description 1
- 241001425418 Devosia neptuniae Species 0.000 description 1
- 229920002245 Dextrose equivalent Polymers 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical class S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 244000309497 Dilophospora alopecuri Species 0.000 description 1
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 1
- 241001559570 Dolichospermum circinale Species 0.000 description 1
- 241000531083 Dolichospermum lemmermannii Species 0.000 description 1
- 241000964127 Dolichospermum planctonicum Species 0.000 description 1
- 241000857977 Dolichospermum viguieri Species 0.000 description 1
- 241000215527 Drechslerella brochopaga Species 0.000 description 1
- 241001262066 Drechslerella dactyloides Species 0.000 description 1
- 241001265056 Engyodontium Species 0.000 description 1
- 241000156885 Ensifer mexicanus Species 0.000 description 1
- 241000149247 Ensifer sojae Species 0.000 description 1
- 241000588914 Enterobacter Species 0.000 description 1
- 241001529716 Entomophaga Species 0.000 description 1
- 241001480508 Entomophthora Species 0.000 description 1
- 241001053078 Erynia Species 0.000 description 1
- 239000004386 Erythritol Substances 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- JIGUQPWFLRLWPJ-UHFFFAOYSA-N Ethyl acrylate Chemical compound CCOC(=O)C=C JIGUQPWFLRLWPJ-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 241001455944 Fibramia thermalis Species 0.000 description 1
- 241000589565 Flavobacterium Species 0.000 description 1
- 241000187809 Frankia Species 0.000 description 1
- 241000187804 Frankia alni Species 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 241000577846 Fusarium dimerum Species 0.000 description 1
- 241000223221 Fusarium oxysporum Species 0.000 description 1
- 241000427940 Fusarium solani Species 0.000 description 1
- 241001626346 Gibellula Species 0.000 description 1
- 241000235500 Gigaspora Species 0.000 description 1
- 241000896533 Gliocladium Species 0.000 description 1
- 241000223247 Gloeocercospora Species 0.000 description 1
- 241000235503 Glomus Species 0.000 description 1
- 241000589236 Gluconobacter Species 0.000 description 1
- JZNWSCPGTDBMEW-UHFFFAOYSA-N Glycerophosphorylethanolamin Natural products NCCOP(O)(=O)OCC(O)CO JZNWSCPGTDBMEW-UHFFFAOYSA-N 0.000 description 1
- ZWZWYGMENQVNFU-UHFFFAOYSA-N Glycerophosphorylserin Natural products OC(=O)C(N)COP(O)(=O)OCC(O)CO ZWZWYGMENQVNFU-UHFFFAOYSA-N 0.000 description 1
- 241000549847 Halospirulina Species 0.000 description 1
- 241000323270 Hammada thomsonii Species 0.000 description 1
- 241000161910 Harposporium Species 0.000 description 1
- 241001626370 Harposporium anguillulae Species 0.000 description 1
- 241000020714 Helicoidea Species 0.000 description 1
- 241001330524 Hesperomyces Species 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 240000001304 Hippeastrum striatum Species 0.000 description 1
- 241000143459 Hirsutella Species 0.000 description 1
- 241000339365 Hirsutella minnesotensis Species 0.000 description 1
- 241000161908 Hirsutella rhossiliensis Species 0.000 description 1
- WOBHKFSMXKNTIM-UHFFFAOYSA-N Hydroxyethyl methacrylate Chemical compound CC(=C)C(=O)OCCO WOBHKFSMXKNTIM-UHFFFAOYSA-N 0.000 description 1
- 241001248588 Hymenostilbe Species 0.000 description 1
- 241000143667 Hypocrella Species 0.000 description 1
- 241000495744 Hypsiboas ericae Species 0.000 description 1
- 241001248590 Isaria Species 0.000 description 1
- AYRXSINWFIIFAE-SCLMCMATSA-N Isomaltose Natural products OC[C@H]1O[C@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)[C@@H](O)[C@@H](O)[C@@H]1O AYRXSINWFIIFAE-SCLMCMATSA-N 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- 241000588749 Klebsiella oxytoca Species 0.000 description 1
- 241000588752 Kluyvera Species 0.000 description 1
- OKPQBUWBBBNTOV-UHFFFAOYSA-N Kojibiose Natural products COC1OC(O)C(OC2OC(OC)C(O)C(O)C2O)C(O)C1O OKPQBUWBBBNTOV-UHFFFAOYSA-N 0.000 description 1
- SKCKOFZKJLZSFA-UHFFFAOYSA-N L-Gulomethylit Natural products CC(O)C(O)C(O)C(O)CO SKCKOFZKJLZSFA-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VSOAQEOCSA-N L-altropyranose Chemical compound OC[C@@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-VSOAQEOCSA-N 0.000 description 1
- 150000000994 L-ascorbates Chemical class 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 241001149568 Laccaria Species 0.000 description 1
- 241001149420 Laccaria bicolor Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 241000235428 Lagenidium Species 0.000 description 1
- 241001121967 Lecanicillium Species 0.000 description 1
- 241000906090 Lecanicillium longisporum Species 0.000 description 1
- 241000321524 Leptolegnia Species 0.000 description 1
- 241001198950 Leptosphaerulina trifolii Species 0.000 description 1
- 240000000604 Lespedeza bicolor Species 0.000 description 1
- 241000193386 Lysinibacillus sphaericus Species 0.000 description 1
- 241000863031 Lysobacter Species 0.000 description 1
- 241001660189 Lysobacter antibioticus Species 0.000 description 1
- 241000863030 Lysobacter enzymogenes Species 0.000 description 1
- 241000584607 Macrospora Species 0.000 description 1
- XJCCHWKNFMUJFE-CGQAXDJHSA-N Maltotriitol Chemical compound O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H](O)[C@@H](O)CO)[C@H](O)CO)O[C@H](CO)[C@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 XJCCHWKNFMUJFE-CGQAXDJHSA-N 0.000 description 1
- NBGXQZRRLOGAJF-UHFFFAOYSA-N Maltulose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)(CO)OCC1O NBGXQZRRLOGAJF-UHFFFAOYSA-N 0.000 description 1
- 244000173804 Martynia lutea Species 0.000 description 1
- 241000767483 Massospora Species 0.000 description 1
- PVXPPJIGRGXGCY-XIOYNQKVSA-N Melibiulose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)C(O)(CO)O1 PVXPPJIGRGXGCY-XIOYNQKVSA-N 0.000 description 1
- 244000207047 Melilotus alba Species 0.000 description 1
- 241000035075 Melithaea ochracea Species 0.000 description 1
- 241000970829 Mesorhizobium Species 0.000 description 1
- 241001001964 Mesorhizobium abyssinicae Species 0.000 description 1
- 241000372475 Mesorhizobium albiziae Species 0.000 description 1
- 241001538060 Mesorhizobium alhagi Species 0.000 description 1
- 241001235187 Mesorhizobium amorphae Species 0.000 description 1
- 241001450667 Mesorhizobium australicum Species 0.000 description 1
- 241001538064 Mesorhizobium camelthorni Species 0.000 description 1
- 241000209428 Mesorhizobium caraganae Species 0.000 description 1
- 241000586758 Mesorhizobium chacoense Species 0.000 description 1
- 241001532588 Mesorhizobium ciceri Species 0.000 description 1
- 241000957052 Mesorhizobium gobiense Species 0.000 description 1
- 241001001972 Mesorhizobium hawassense Species 0.000 description 1
- 241001135320 Mesorhizobium huakuii Species 0.000 description 1
- 241000589195 Mesorhizobium loti Species 0.000 description 1
- 241000158155 Mesorhizobium mediterraneum Species 0.000 description 1
- 241000286775 Mesorhizobium metallidurans Species 0.000 description 1
- 241001515438 Mesorhizobium muleiense Species 0.000 description 1
- 241000292853 Mesorhizobium opportunistum Species 0.000 description 1
- 241000973026 Mesorhizobium plurifarium Species 0.000 description 1
- 241000108789 Mesorhizobium qingshengii Species 0.000 description 1
- 241000551303 Mesorhizobium robiniae Species 0.000 description 1
- 241000348443 Mesorhizobium sangaii Species 0.000 description 1
- 241000973898 Mesorhizobium septentrionale Species 0.000 description 1
- 241001101775 Mesorhizobium shangrilense Species 0.000 description 1
- 241001001996 Mesorhizobium shonense Species 0.000 description 1
- 241000659891 Mesorhizobium silamurunense Species 0.000 description 1
- 241001370285 Mesorhizobium tamadayense Species 0.000 description 1
- 241000957050 Mesorhizobium tarimense Species 0.000 description 1
- 241000973895 Mesorhizobium temperatum Species 0.000 description 1
- 241000892210 Mesorhizobium thiogangeticum Species 0.000 description 1
- 241001534001 Mesorhizobium tianshanense Species 0.000 description 1
- 241000223201 Metarhizium Species 0.000 description 1
- 241000289203 Metarhizium brunneum Species 0.000 description 1
- 241000318908 Metarhizium flavoviride Species 0.000 description 1
- 241001303988 Metarhizium rileyi Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- GYCMBHHDWRMZGG-UHFFFAOYSA-N Methylacrylonitrile Chemical compound CC(=C)C#N GYCMBHHDWRMZGG-UHFFFAOYSA-N 0.000 description 1
- 241000589323 Methylobacterium Species 0.000 description 1
- 241000342654 Methylobacterium adhaesivum Species 0.000 description 1
- 241001037133 Methylobacterium aerolatum Species 0.000 description 1
- 241000135180 Methylobacterium aminovorans Species 0.000 description 1
- 241000408736 Methylobacterium aquaticum Species 0.000 description 1
- 241000134180 Methylobacterium brachiatum Species 0.000 description 1
- 241000515910 Methylobacterium brachythecii Species 0.000 description 1
- 241001175057 Methylobacterium bullatum Species 0.000 description 1
- 241000256076 Methylobacterium cerastii Species 0.000 description 1
- 241000821682 Methylobacterium dankookense Species 0.000 description 1
- 241001542929 Methylobacterium fujisawaense Species 0.000 description 1
- 241000204576 Methylobacterium gnaphalii Species 0.000 description 1
- 241001000998 Methylobacterium goesingense Species 0.000 description 1
- 241001587124 Methylobacterium gossipiicola Species 0.000 description 1
- 241000143752 Methylobacterium gregans Species 0.000 description 1
- 241000515912 Methylobacterium haplocladii Species 0.000 description 1
- 241000408722 Methylobacterium hispanicum Species 0.000 description 1
- 241001287913 Methylobacterium isbiliense Species 0.000 description 1
- 241000481564 Methylobacterium jeotgali Species 0.000 description 1
- 241000143750 Methylobacterium komagatae Species 0.000 description 1
- 241000328076 Methylobacterium longum Species 0.000 description 1
- 241001249758 Methylobacterium lusitanum Species 0.000 description 1
- 241001676813 Methylobacterium marchantiae Species 0.000 description 1
- 241001535042 Methylobacterium mesophilicum Species 0.000 description 1
- 241000197701 Methylobacterium nodulans Species 0.000 description 1
- 241000589339 Methylobacterium organophilum Species 0.000 description 1
- 241000647037 Methylobacterium oxalidis Species 0.000 description 1
- 241000143742 Methylobacterium persicinum Species 0.000 description 1
- 241001038652 Methylobacterium phyllosphaerae Species 0.000 description 1
- 241001391199 Methylobacterium platani Species 0.000 description 1
- 241001145052 Methylobacterium podarium Species 0.000 description 1
- 241000272433 Methylobacterium populi Species 0.000 description 1
- 241001430258 Methylobacterium radiotolerans Species 0.000 description 1
- 241001148217 Methylobacterium rhodesianum Species 0.000 description 1
- 241001148218 Methylobacterium rhodinum Species 0.000 description 1
- 241001001815 Methylobacterium salsuginis Species 0.000 description 1
- 241000494106 Methylobacterium soli Species 0.000 description 1
- 241001288657 Methylobacterium suomiense Species 0.000 description 1
- 241000143759 Methylobacterium tardum Species 0.000 description 1
- 241000860208 Methylobacterium tarhaniae Species 0.000 description 1
- 241000219277 Methylobacterium thiocyanatum Species 0.000 description 1
- 241001529815 Methylobacterium trifolii Species 0.000 description 1
- 241001206627 Methylobacterium variabile Species 0.000 description 1
- 241001148213 Methylobacterium zatmanii Species 0.000 description 1
- 241001123674 Metschnikowia Species 0.000 description 1
- 241001508001 Microbacterium laevaniformans Species 0.000 description 1
- 241001542780 Microsphaeropsis Species 0.000 description 1
- 241001112070 Microvirga Species 0.000 description 1
- 241000897456 Microvirga aerilata Species 0.000 description 1
- 241000897453 Microvirga aerophila Species 0.000 description 1
- 241001611005 Microvirga flocculans Species 0.000 description 1
- 241000189600 Microvirga guangxiensis Species 0.000 description 1
- 241001677105 Microvirga lotononidis Species 0.000 description 1
- 241001309472 Microvirga lupini Species 0.000 description 1
- 241001112069 Microvirga subterranea Species 0.000 description 1
- 241000053504 Microvirga vignae Species 0.000 description 1
- 241000362874 Microvirga zambiensis Species 0.000 description 1
- 241000529935 Monacrosporium Species 0.000 description 1
- 241000586196 Monacrosporium asterospermum Species 0.000 description 1
- 241001518731 Monilinia fructicola Species 0.000 description 1
- 241000883792 Mucilaginibacter roseus Species 0.000 description 1
- 241000235395 Mucor Species 0.000 description 1
- 241001645776 Muscodor Species 0.000 description 1
- 241001326504 Myriangium Species 0.000 description 1
- 240000004839 Myristica iners Species 0.000 description 1
- 241000223251 Myrothecium Species 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- 241001226034 Nectria <echinoderm> Species 0.000 description 1
- 241000215425 Nematoctonus Species 0.000 description 1
- 241001492280 Nematoctonus geogenius Species 0.000 description 1
- 241000411011 Nematoctonus leiosporus Species 0.000 description 1
- 241001265860 Neoascotaiwania limnetica Species 0.000 description 1
- 241001097618 Neorhizobium alkalisoli Species 0.000 description 1
- 241000589125 Neorhizobium galegae Species 0.000 description 1
- 241000948290 Neorhizobium huautlense Species 0.000 description 1
- 241001626373 Neozygites Species 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 241001134691 Nitrospirillum amazonense Species 0.000 description 1
- 241001478326 Niveispirillum irakense Species 0.000 description 1
- 241000157296 Nomuraea Species 0.000 description 1
- 241000761291 Nostoc caeruleum Species 0.000 description 1
- 241000146914 Nostoc carneum Species 0.000 description 1
- 240000001131 Nostoc commune Species 0.000 description 1
- 240000005303 Nostoc ellipsosporum Species 0.000 description 1
- 241000692932 Nostoc flagelliforme Species 0.000 description 1
- 241000320437 Nostoc linckia Species 0.000 description 1
- 241000301264 Nostoc microscopicum Species 0.000 description 1
- 241001554910 Nostoc paludosum Species 0.000 description 1
- 241000761326 Nostoc pruniforme Species 0.000 description 1
- 241001611244 Nostoc sphaericum Species 0.000 description 1
- 241000452732 Nostoc sphaeroides Species 0.000 description 1
- 241000315969 Nostoc spongiaeforme Species 0.000 description 1
- 241001118196 Nostoc verrucosum Species 0.000 description 1
- DKXNBNKWCZZMJT-UHFFFAOYSA-N O4-alpha-D-Mannopyranosyl-D-mannose Natural products O=CC(O)C(O)C(C(O)CO)OC1OC(CO)C(O)C(O)C1O DKXNBNKWCZZMJT-UHFFFAOYSA-N 0.000 description 1
- AYRXSINWFIIFAE-UHFFFAOYSA-N O6-alpha-D-Galactopyranosyl-D-galactose Natural products OCC1OC(OCC(O)C(O)C(O)C(O)C=O)C(O)C(O)C1O AYRXSINWFIIFAE-UHFFFAOYSA-N 0.000 description 1
- 241000588843 Ochrobactrum Species 0.000 description 1
- 241000588814 Ochrobactrum anthropi Species 0.000 description 1
- 241000665924 Ochrobactrum cytisi Species 0.000 description 1
- 241000279010 Ochrobactrum daejeonense Species 0.000 description 1
- 241001018489 Ochrobactrum guangzhouense Species 0.000 description 1
- 241000359249 Ochrobactrum intermedium Species 0.000 description 1
- 241001376793 Ochrobactrum lupini Species 0.000 description 1
- 241000373064 Ochrobactrum oryzae Species 0.000 description 1
- 241001188780 Ochrobactrum pituitosum Species 0.000 description 1
- 241001188758 Ochrobactrum rhizosphaerae Species 0.000 description 1
- 241001188759 Ochrobactrum thiophenivorans Species 0.000 description 1
- 241000359248 Ochrobactrum tritici Species 0.000 description 1
- 241001310945 Oidiodendron Species 0.000 description 1
- 241001236817 Paecilomyces <Clavicipitaceae> Species 0.000 description 1
- 241000179039 Paenibacillus Species 0.000 description 1
- 241000178959 Paenibacillus durus Species 0.000 description 1
- 241000233727 Paenibacillus koreensis Species 0.000 description 1
- 241000194105 Paenibacillus polymyxa Species 0.000 description 1
- 241000345871 Pandoraea norimbergensis Species 0.000 description 1
- 241000520272 Pantoea Species 0.000 description 1
- 241000588912 Pantoea agglomerans Species 0.000 description 1
- 241001480343 Pantoea vagans Species 0.000 description 1
- 241000525892 Paraburkholderia acidipaludis Species 0.000 description 1
- 241001043214 Paraburkholderia azotifigens Species 0.000 description 1
- 241000299079 Paraburkholderia bannensis Species 0.000 description 1
- 241001306959 Paraburkholderia bryophila Species 0.000 description 1
- 241000930993 Paraburkholderia caledonica Species 0.000 description 1
- 241001274695 Paraburkholderia caribensis Species 0.000 description 1
- 241001135514 Paraburkholderia caryophylli Species 0.000 description 1
- 241000617705 Paraburkholderia diazotrophica Species 0.000 description 1
- 241000690958 Paraburkholderia dilworthii Species 0.000 description 1
- 241000901911 Paraburkholderia eburnea Species 0.000 description 1
- 241001029413 Paraburkholderia endofungorum Species 0.000 description 1
- 241000534281 Paraburkholderia ferrariae Species 0.000 description 1
- 241000930992 Paraburkholderia fungorum Species 0.000 description 1
- 241001238056 Paraburkholderia ginsengisoli Species 0.000 description 1
- 241000866630 Paraburkholderia graminis Species 0.000 description 1
- 241001132135 Paraburkholderia heleia Species 0.000 description 1
- 241000448183 Paraburkholderia kururiensis Species 0.000 description 1
- 241001306960 Paraburkholderia megapolitana Species 0.000 description 1
- 241001239616 Paraburkholderia mimosarum Species 0.000 description 1
- 241000851467 Paraburkholderia nodosa Species 0.000 description 1
- 241000866634 Paraburkholderia phenazinium Species 0.000 description 1
- 241001346801 Paraburkholderia phenoliruptrix Species 0.000 description 1
- 241001343907 Paraburkholderia phymatum Species 0.000 description 1
- 241000037463 Paraburkholderia phytofirmans Species 0.000 description 1
- 241000973051 Paraburkholderia rhizoxinica Species 0.000 description 1
- 241000261185 Paraburkholderia rhynchosiae Species 0.000 description 1
- 241000435839 Paraburkholderia sabiae Species 0.000 description 1
- 241000056981 Paraburkholderia sacchari Species 0.000 description 1
- 241001648273 Paraburkholderia sartisoli Species 0.000 description 1
- 241000841738 Paraburkholderia sediminicola Species 0.000 description 1
- 241001615782 Paraburkholderia silvatlantica Species 0.000 description 1
- 241000480193 Paraburkholderia soli Species 0.000 description 1
- 241000690959 Paraburkholderia sprentiae Species 0.000 description 1
- 241001660015 Paraburkholderia symbiotica Species 0.000 description 1
- 241001238057 Paraburkholderia terrae Species 0.000 description 1
- 241000428237 Paraburkholderia terricola Species 0.000 description 1
- 241000318919 Paraburkholderia tropica Species 0.000 description 1
- 241000040850 Paraburkholderia tuberum Species 0.000 description 1
- 241001638083 Paraburkholderia unamae Species 0.000 description 1
- 241001509383 Paraburkholderia xenovorans Species 0.000 description 1
- 239000005662 Paraffin oil Substances 0.000 description 1
- 241000937092 Paraisaria Species 0.000 description 1
- 241000887182 Paraphaeosphaeria minitans Species 0.000 description 1
- 241000222291 Passalora fulva Species 0.000 description 1
- 241001242657 Pasteuria nishizawae Species 0.000 description 1
- 241001668578 Pasteuria penetrans Species 0.000 description 1
- 241001022716 Pedicularis pinetorum Species 0.000 description 1
- 241001507683 Penicillium aurantiogriseum Species 0.000 description 1
- 241000228145 Penicillium brevicompactum Species 0.000 description 1
- 241000613981 Penicillium brevicompactum AgRF18 Species 0.000 description 1
- 241000228172 Penicillium canescens Species 0.000 description 1
- 241001025446 Penicillium canescens ATCC 10419 Species 0.000 description 1
- 241000985553 Penicillium citreonigrum Species 0.000 description 1
- 241000228153 Penicillium citrinum Species 0.000 description 1
- 241001507673 Penicillium digitatum Species 0.000 description 1
- 241001025481 Penicillium expansum ATCC 24692 Species 0.000 description 1
- 241001341981 Penicillium expansum YT02 Species 0.000 description 1
- 241001219824 Penicillium fuscum Species 0.000 description 1
- 241001025448 Penicillium glabrum DAOM 239074 Species 0.000 description 1
- 241000228127 Penicillium griseofulvum Species 0.000 description 1
- 241000985532 Penicillium implicatum Species 0.000 description 1
- 241000519895 Penicillium janczewskii Species 0.000 description 1
- 241000663552 Penicillium janthinellum ATCC 10455 Species 0.000 description 1
- 241000674483 Penicillium lanosocoeruleum Species 0.000 description 1
- 241000482868 Penicillium lanosocoeruleum ATCC 48919 Species 0.000 description 1
- 241001549762 Penicillium montanense Species 0.000 description 1
- 241000985513 Penicillium oxalicum Species 0.000 description 1
- 241000985516 Penicillium raistrickii Species 0.000 description 1
- 241000614003 Penicillium raistrickii ATCC 10490 Species 0.000 description 1
- 241000864268 Penicillium solitum Species 0.000 description 1
- 241001219820 Penicillium velutinum Species 0.000 description 1
- 241000864371 Penicillium viridicatum Species 0.000 description 1
- 241001622184 Peperomia meridana Species 0.000 description 1
- 244000126361 Phalaenopsis gigantea Species 0.000 description 1
- 241001634117 Phlebiopsis Species 0.000 description 1
- 241001148062 Photorhabdus Species 0.000 description 1
- 241001135342 Phyllobacterium Species 0.000 description 1
- 241001272744 Phyllobacterium bourgognense Species 0.000 description 1
- 241000769453 Phyllobacterium catacumbae Species 0.000 description 1
- 241001100182 Phyllobacterium endophyticum Species 0.000 description 1
- 241001272748 Phyllobacterium ifriqiyense Species 0.000 description 1
- 241000090854 Phyllobacterium loti Species 0.000 description 1
- 241001135317 Phyllobacterium myrsinacearum Species 0.000 description 1
- 241000773005 Phyllobacterium sophorae Species 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 241001503957 Pisolithus Species 0.000 description 1
- 241000913090 Planktothricoides Species 0.000 description 1
- 240000002924 Platycladus orientalis Species 0.000 description 1
- 241000192665 Plectonema Species 0.000 description 1
- 241001092921 Plocamaphis flocculosa Species 0.000 description 1
- 241001002196 Pochonia Species 0.000 description 1
- 241000805555 Podonectria Species 0.000 description 1
- RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 description 1
- 241001626344 Polycephalomyces Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 229920000604 Polyethylene Glycol 200 Polymers 0.000 description 1
- 229920002556 Polyethylene Glycol 300 Polymers 0.000 description 1
- 229920002565 Polyethylene Glycol 400 Polymers 0.000 description 1
- 239000004721 Polyphenylene oxide Substances 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 229920001219 Polysorbate 40 Polymers 0.000 description 1
- 229920001214 Polysorbate 60 Polymers 0.000 description 1
- 244000041809 Prenanthes striata Species 0.000 description 1
- 241000192141 Prochloron Species 0.000 description 1
- 241000192139 Prochloron didemni Species 0.000 description 1
- 241000192144 Prochlorothrix Species 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
- 241000657242 Protohermes lii Species 0.000 description 1
- 241001626311 Pseudogibellula Species 0.000 description 1
- 241000204715 Pseudomonas agarici Species 0.000 description 1
- 241000218935 Pseudomonas azotoformans Species 0.000 description 1
- 241001279845 Pseudomonas balearica Species 0.000 description 1
- 241001365263 Pseudomonas blatchfordae Species 0.000 description 1
- 241000855937 Pseudomonas borbori Species 0.000 description 1
- 241000620655 Pseudomonas brenneri Species 0.000 description 1
- 241000007104 Pseudomonas cannabina Species 0.000 description 1
- 241000180027 Pseudomonas cedrina Species 0.000 description 1
- 241001646398 Pseudomonas chlororaphis Species 0.000 description 1
- 241001645955 Pseudomonas chlororaphis subsp. aureofaciens Species 0.000 description 1
- 241001144911 Pseudomonas congelans Species 0.000 description 1
- 241000218936 Pseudomonas corrugata Species 0.000 description 1
- 241000425890 Pseudomonas costantinii Species 0.000 description 1
- 241001253546 Pseudomonas entomophila Species 0.000 description 1
- 241000589540 Pseudomonas fluorescens Species 0.000 description 1
- 241000490004 Pseudomonas fuscovaginae Species 0.000 description 1
- 241001312498 Pseudomonas gessardii Species 0.000 description 1
- 241000913726 Pseudomonas kilonensis Species 0.000 description 1
- 241001277052 Pseudomonas libanensis Species 0.000 description 1
- 241001670039 Pseudomonas lundensis Species 0.000 description 1
- 241000218905 Pseudomonas luteola Species 0.000 description 1
- 241001277679 Pseudomonas mandelii Species 0.000 description 1
- 241000589537 Pseudomonas marginalis Species 0.000 description 1
- 241001312486 Pseudomonas migulae Species 0.000 description 1
- 241001615563 Pseudomonas moraviensis Species 0.000 description 1
- 241000204709 Pseudomonas mucidolens Species 0.000 description 1
- 241000218904 Pseudomonas oryzihabitans Species 0.000 description 1
- 241001425590 Pseudomonas palleroniana Species 0.000 description 1
- 241000954716 Pseudomonas panacis Species 0.000 description 1
- 241000039933 Pseudomonas parafulva Species 0.000 description 1
- 241000281856 Pseudomonas peli Species 0.000 description 1
- 241001670066 Pseudomonas pertucinogena Species 0.000 description 1
- 241001223182 Pseudomonas plecoglossicida Species 0.000 description 1
- 241001641542 Pseudomonas proteolytica Species 0.000 description 1
- 241000589776 Pseudomonas putida Species 0.000 description 1
- 241001291486 Pseudomonas rhodesiae Species 0.000 description 1
- 241000589614 Pseudomonas stutzeri Species 0.000 description 1
- 241000218902 Pseudomonas synxantha Species 0.000 description 1
- 241000589615 Pseudomonas syringae Species 0.000 description 1
- 241000218903 Pseudomonas taetrolens Species 0.000 description 1
- 241001148199 Pseudomonas tolaasii Species 0.000 description 1
- 241001291485 Pseudomonas veronii Species 0.000 description 1
- 241000893045 Pseudozyma Species 0.000 description 1
- 244000040267 Psychotria aurantiaca Species 0.000 description 1
- 241000233639 Pythium Species 0.000 description 1
- 241000131360 Pythium oligandrum Species 0.000 description 1
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 1
- 241000589625 Ralstonia pickettii Species 0.000 description 1
- 241000589180 Rhizobium Species 0.000 description 1
- 241001478328 Rhizobium aggregatum Species 0.000 description 1
- 241000563500 Rhizobium alamii Species 0.000 description 1
- 241001008296 Rhizobium calliandrae Species 0.000 description 1
- 241000155185 Rhizobium cauense Species 0.000 description 1
- 241000865252 Rhizobium cellulosilyticum Species 0.000 description 1
- 241000972381 Rhizobium daejeonense Species 0.000 description 1
- 241001182163 Rhizobium endolithicum Species 0.000 description 1
- 241001269866 Rhizobium endophyticum Species 0.000 description 1
- 241001148115 Rhizobium etli Species 0.000 description 1
- 241001206173 Rhizobium fabae Species 0.000 description 1
- 241000820597 Rhizobium freirei Species 0.000 description 1
- 241000556551 Rhizobium gallicum Species 0.000 description 1
- 241000556569 Rhizobium giardinii Species 0.000 description 1
- 241000284821 Rhizobium grahamii Species 0.000 description 1
- 241000508774 Rhizobium hainanense Species 0.000 description 1
- 241000857656 Rhizobium halophytocola Species 0.000 description 1
- 241000388829 Rhizobium halotolerans Species 0.000 description 1
- 241001165982 Rhizobium helanshanense Species 0.000 description 1
- 241000387997 Rhizobium herbae Species 0.000 description 1
- 241000043570 Rhizobium indigoferae Species 0.000 description 1
- 241001008297 Rhizobium jaguaris Species 0.000 description 1
- 241001666026 Rhizobium kunmingense Species 0.000 description 1
- 241000785454 Rhizobium laguerreae Species 0.000 description 1
- 241001308669 Rhizobium lemnae Species 0.000 description 1
- 241001148119 Rhizobium leucaenae Species 0.000 description 1
- 241000043559 Rhizobium loessense Species 0.000 description 1
- 241001149656 Rhizobium lusitanum Species 0.000 description 1
- 241001008294 Rhizobium mayense Species 0.000 description 1
- 241000807007 Rhizobium mesoamericanum Species 0.000 description 1
- 241000372606 Rhizobium mesosinicum Species 0.000 description 1
- 241000959895 Rhizobium miluonense Species 0.000 description 1
- 241000580866 Rhizobium mongolense Species 0.000 description 1
- 241000957023 Rhizobium multihospitium Species 0.000 description 1
- 241001662908 Rhizobium naphthalenivorans Species 0.000 description 1
- 241000980512 Rhizobium nepotum Species 0.000 description 1
- 241001144606 Rhizobium oryzae Species 0.000 description 1
- 241000323264 Rhizobium pakistanense Species 0.000 description 1
- 241000978766 Rhizobium paknamense Species 0.000 description 1
- 241001579961 Rhizobium paranaense Species 0.000 description 1
- 241000589126 Rhizobium phaseoli Species 0.000 description 1
- 241001216644 Rhizobium pisi Species 0.000 description 1
- 241001622061 Rhizobium pongamiae Species 0.000 description 1
- 241001005816 Rhizobium populi Species 0.000 description 1
- 241000212574 Rhizobium pseudoryzae Species 0.000 description 1
- 241001569982 Rhizobium pusense Species 0.000 description 1
- 241000758566 Rhizobium rhizoryzae Species 0.000 description 1
- 241000628209 Rhizobium smilacinae Species 0.000 description 1
- 241001353184 Rhizobium soli Species 0.000 description 1
- 241001159613 Rhizobium sophorae Species 0.000 description 1
- 241001159582 Rhizobium sophoriradicis Species 0.000 description 1
- 241000821033 Rhizobium sphaerophysae Species 0.000 description 1
- 241000309946 Rhizobium straminoryzae Species 0.000 description 1
- 241000894031 Rhizobium subbaraonis Species 0.000 description 1
- 241000489997 Rhizobium sullae Species 0.000 description 1
- 241000765952 Rhizobium taibaishanense Species 0.000 description 1
- 241000803569 Rhizobium tarimense Species 0.000 description 1
- 241000303321 Rhizobium tibeticum Species 0.000 description 1
- 241000589124 Rhizobium tropici Species 0.000 description 1
- 241000226064 Rhizobium tubonense Species 0.000 description 1
- 241001312560 Rhizobium undicola Species 0.000 description 1
- 241001132465 Rhizobium vallis Species 0.000 description 1
- 241001639233 Rhizobium vignae Species 0.000 description 1
- 241000861993 Rhizobium yanglingense Species 0.000 description 1
- 241001175365 Rhizobium yantingense Species 0.000 description 1
- 241001361634 Rhizoctonia Species 0.000 description 1
- 241000214030 Rhizophagus fasciculatus Species 0.000 description 1
- 241000316848 Rhodococcus <scale insect> Species 0.000 description 1
- 240000007967 Rhododendron lapponicum Species 0.000 description 1
- 240000008199 Rhododendron molle Species 0.000 description 1
- 240000004594 Ribes aureum Species 0.000 description 1
- JVWLUVNSQYXYBE-UHFFFAOYSA-N Ribitol Natural products OCC(C)C(O)C(O)CO JVWLUVNSQYXYBE-UHFFFAOYSA-N 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 241001135520 Robbsia andropogonis Species 0.000 description 1
- OVVGHDNPYGTYIT-VHBGUFLRSA-N Robinobiose Natural products O(C[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)O1)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](C)O1 OVVGHDNPYGTYIT-VHBGUFLRSA-N 0.000 description 1
- 241000187560 Saccharopolyspora Species 0.000 description 1
- 206010039710 Scleroderma Diseases 0.000 description 1
- 241000221394 Septobasidium Species 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 240000007012 Setaria adhaerens Species 0.000 description 1
- 241001647968 Shinella Species 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 241001302442 Sinorhizobium abri Species 0.000 description 1
- 241001291528 Sinorhizobium arboris Species 0.000 description 1
- 241000301578 Sinorhizobium chiapanecum Species 0.000 description 1
- 241000369999 Sinorhizobium fredii USDA 205 Species 0.000 description 1
- 241001302482 Sinorhizobium indiaense Species 0.000 description 1
- 241001291531 Sinorhizobium kostiense Species 0.000 description 1
- 241000143665 Sinorhizobium medicae Species 0.000 description 1
- 241000589196 Sinorhizobium meliloti Species 0.000 description 1
- 241001509304 Sinorhizobium saheli Species 0.000 description 1
- 241000143663 Sinorhizobium terangae Species 0.000 description 1
- 241001135313 Sinorhizobium xinjiangense Species 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 240000002307 Solanum ptychanthum Species 0.000 description 1
- HIWPGCMGAMJNRG-ACCAVRKYSA-N Sophorose Natural products O([C@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HIWPGCMGAMJNRG-ACCAVRKYSA-N 0.000 description 1
- 241001626293 Sorosporella Species 0.000 description 1
- 241001644970 Sphaerodes Species 0.000 description 1
- 241000626935 Sphaerodes mycoparasitica Species 0.000 description 1
- 241000256248 Spodoptera Species 0.000 description 1
- 240000003949 Sporobolus virginicus Species 0.000 description 1
- 241001450879 Sporodiniella Species 0.000 description 1
- 241001464969 Sporolactobacillus laevolacticus Species 0.000 description 1
- 241000193395 Sporosarcina pasteurii Species 0.000 description 1
- 241000779850 Stagonospora tainanensis Species 0.000 description 1
- 241001480238 Steinernema Species 0.000 description 1
- 241001480223 Steinernema carpocapsae Species 0.000 description 1
- 241000509371 Steinernema feltiae Species 0.000 description 1
- 241000706285 Steinernema kraussei Species 0.000 description 1
- 241000514831 Stemphylium botryosum Species 0.000 description 1
- 241000122971 Stenotrophomonas Species 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241000186990 Streptomyces cacaoi Species 0.000 description 1
- 241001467541 Streptomyces galbus Species 0.000 description 1
- 241000191251 Streptomyces griseoviridis Species 0.000 description 1
- 241000218483 Streptomyces lydicus Species 0.000 description 1
- 241000970854 Streptomyces violaceusniger Species 0.000 description 1
- 241000203590 Streptosporangium Species 0.000 description 1
- 240000000591 Strychnos spinosa Species 0.000 description 1
- 241001248469 Swaminathania Species 0.000 description 1
- 241001515930 Synaptothrips psoraleae Species 0.000 description 1
- 241000228341 Talaromyces Species 0.000 description 1
- 241001507810 Talaromyces aculeatus Species 0.000 description 1
- 241000228343 Talaromyces flavus Species 0.000 description 1
- 241001136556 Talaromyces minioluteus Species 0.000 description 1
- 241000606507 Talaromyces pinophilus Species 0.000 description 1
- 241001540751 Talaromyces ruber Species 0.000 description 1
- 241000519854 Talaromyces rugulosus Species 0.000 description 1
- 241001136559 Talaromyces variabilis Species 0.000 description 1
- 241001137073 Thaumatotibia leucotreta Species 0.000 description 1
- 241000605118 Thiobacillus Species 0.000 description 1
- 241001626284 Tilachlidium Species 0.000 description 1
- 241001149964 Tolypocladium Species 0.000 description 1
- 241000157473 Tolypothrix Species 0.000 description 1
- 241001538101 Torrubiella <Clavicipitaceae> Species 0.000 description 1
- XSTXAVWGXDQKEL-UHFFFAOYSA-N Trichloroethylene Chemical group ClC=C(Cl)Cl XSTXAVWGXDQKEL-UHFFFAOYSA-N 0.000 description 1
- 241000944293 Trichoderma gamsii Species 0.000 description 1
- 241000123975 Trichoderma polysporum Species 0.000 description 1
- 241000385222 Trichoderma stromaticum Species 0.000 description 1
- 241000192540 Trichormus azollae Species 0.000 description 1
- WGLPBDUCMAPZCE-UHFFFAOYSA-N Trioxochromium Chemical compound O=[Cr](=O)=O WGLPBDUCMAPZCE-UHFFFAOYSA-N 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- DRQXUCVJDCRJDB-UHFFFAOYSA-N Turanose Natural products OC1C(CO)OC(O)(CO)C1OC1C(O)C(O)C(O)C(CO)O1 DRQXUCVJDCRJDB-UHFFFAOYSA-N 0.000 description 1
- 241000959260 Typhula Species 0.000 description 1
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 1
- 241000266300 Ulocladium Species 0.000 description 1
- 241001107952 Uredinella Species 0.000 description 1
- 206010046865 Vaccinia virus infection Diseases 0.000 description 1
- 241001478283 Variovorax Species 0.000 description 1
- 241000082085 Verticillium <Phyllachorales> Species 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 244000207951 Vigna marina Species 0.000 description 1
- XTXRWKRVRITETP-UHFFFAOYSA-N Vinyl acetate Chemical compound CC(=O)OC=C XTXRWKRVRITETP-UHFFFAOYSA-N 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 244000068697 Vitis rotundifolia Species 0.000 description 1
- 235000006359 Vitis rotundifolia var rotundifolia Nutrition 0.000 description 1
- 241000589506 Xanthobacter Species 0.000 description 1
- 241000589634 Xanthomonas Species 0.000 description 1
- 241000607734 Yersinia <bacteria> Species 0.000 description 1
- 241001480503 Zoophthora Species 0.000 description 1
- 241000192351 [Candida] oleophila Species 0.000 description 1
- 241000192248 [Candida] saitoana Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 125000002877 alkyl aryl group Chemical group 0.000 description 1
- 150000004996 alkyl benzenes Chemical class 0.000 description 1
- 125000005599 alkyl carboxylate group Chemical group 0.000 description 1
- 150000005215 alkyl ethers Chemical class 0.000 description 1
- 150000008051 alkyl sulfates Chemical class 0.000 description 1
- 229940045714 alkyl sulfonate alkylating agent Drugs 0.000 description 1
- 150000008052 alkyl sulfonates Chemical class 0.000 description 1
- 125000005211 alkyl trimethyl ammonium group Chemical group 0.000 description 1
- 229940087168 alpha tocopherol Drugs 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- SRBFZHDQGSBBOR-STGXQOJASA-N alpha-D-lyxopyranose Chemical compound O[C@@H]1CO[C@H](O)[C@@H](O)[C@H]1O SRBFZHDQGSBBOR-STGXQOJASA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 150000001413 amino acids Chemical group 0.000 description 1
- 150000008064 anhydrides Chemical class 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 239000008365 aqueous carrier Substances 0.000 description 1
- 229960000892 attapulgite Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 101150058790 bcp gene Proteins 0.000 description 1
- 235000013871 bee wax Nutrition 0.000 description 1
- 239000012166 beeswax Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 229960000686 benzalkonium chloride Drugs 0.000 description 1
- SRSXLGNVWSONIS-UHFFFAOYSA-N benzenesulfonic acid Chemical class OS(=O)(=O)C1=CC=CC=C1 SRSXLGNVWSONIS-UHFFFAOYSA-N 0.000 description 1
- UREZNYTWGJKWBI-UHFFFAOYSA-M benzethonium chloride Chemical compound [Cl-].C1=CC(C(C)(C)CC(C)(C)C)=CC=C1OCCOCC[N+](C)(C)CC1=CC=CC=C1 UREZNYTWGJKWBI-UHFFFAOYSA-M 0.000 description 1
- 229960001950 benzethonium chloride Drugs 0.000 description 1
- CADWTSSKOVRVJC-UHFFFAOYSA-N benzyl(dimethyl)azanium;chloride Chemical compound [Cl-].C[NH+](C)CC1=CC=CC=C1 CADWTSSKOVRVJC-UHFFFAOYSA-N 0.000 description 1
- QLTSDROPCWIKKY-PMCTYKHCSA-N beta-D-glucosaminyl-(1->4)-beta-D-glucosamine Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O)[C@@H](CO)O1 QLTSDROPCWIKKY-PMCTYKHCSA-N 0.000 description 1
- DLRVVLDZNNYCBX-ZZFZYMBESA-N beta-melibiose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)O1 DLRVVLDZNNYCBX-ZZFZYMBESA-N 0.000 description 1
- HIWPGCMGAMJNRG-UHFFFAOYSA-N beta-sophorose Natural products OC1C(O)C(CO)OC(O)C1OC1C(O)C(O)C(O)C(CO)O1 HIWPGCMGAMJNRG-UHFFFAOYSA-N 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000000853 biopesticidal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- XVBRCOKDZVQYAY-UHFFFAOYSA-N bronidox Chemical compound [O-][N+](=O)C1(Br)COCOC1 XVBRCOKDZVQYAY-UHFFFAOYSA-N 0.000 description 1
- 239000010974 bronze Substances 0.000 description 1
- JIJAYWGYIDJVJI-UHFFFAOYSA-N butyl naphthalene-1-sulfonate Chemical class C1=CC=C2C(S(=O)(=O)OCCCC)=CC=CC2=C1 JIJAYWGYIDJVJI-UHFFFAOYSA-N 0.000 description 1
- UDHMTPILEWBIQI-UHFFFAOYSA-N butyl naphthalene-1-sulfonate;sodium Chemical compound [Na].C1=CC=C2C(S(=O)(=O)OCCCC)=CC=CC2=C1 UDHMTPILEWBIQI-UHFFFAOYSA-N 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 235000010216 calcium carbonate Nutrition 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229920005551 calcium lignosulfonate Polymers 0.000 description 1
- CJZGTCYPCWQAJB-UHFFFAOYSA-L calcium stearate Chemical compound [Ca+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O CJZGTCYPCWQAJB-UHFFFAOYSA-L 0.000 description 1
- 235000013539 calcium stearate Nutrition 0.000 description 1
- 239000008116 calcium stearate Substances 0.000 description 1
- RYAGRZNBULDMBW-UHFFFAOYSA-L calcium;3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Ca+2].COC1=CC=CC(CC(CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O RYAGRZNBULDMBW-UHFFFAOYSA-L 0.000 description 1
- 239000004204 candelilla wax Substances 0.000 description 1
- 235000013868 candelilla wax Nutrition 0.000 description 1
- 229940073532 candelilla wax Drugs 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 150000001733 carboxylic acid esters Chemical class 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 229940082500 cetostearyl alcohol Drugs 0.000 description 1
- 229960000800 cetrimonium bromide Drugs 0.000 description 1
- 229960000541 cetyl alcohol Drugs 0.000 description 1
- 229960001927 cetylpyridinium chloride Drugs 0.000 description 1
- YMKDRGPMQRFJGP-UHFFFAOYSA-M cetylpyridinium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCC[N+]1=CC=CC=C1 YMKDRGPMQRFJGP-UHFFFAOYSA-M 0.000 description 1
- WOWHHFRSBJGXCM-UHFFFAOYSA-M cetyltrimethylammonium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCC[N+](C)(C)C WOWHHFRSBJGXCM-UHFFFAOYSA-M 0.000 description 1
- YACLQRRMGMJLJV-UHFFFAOYSA-N chloroprene Chemical compound ClC(=C)C=C YACLQRRMGMJLJV-UHFFFAOYSA-N 0.000 description 1
- 229910000423 chromium oxide Inorganic materials 0.000 description 1
- WBYWAXJHAXSJNI-UHFFFAOYSA-N cinnamic acid Chemical class OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 description 1
- 239000002734 clay mineral Substances 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- MRUAUOIMASANKQ-UHFFFAOYSA-N cocamidopropyl betaine Chemical compound CCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC([O-])=O MRUAUOIMASANKQ-UHFFFAOYSA-N 0.000 description 1
- 229940073507 cocamidopropyl betaine Drugs 0.000 description 1
- 238000007398 colorimetric assay Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- KUNSUQLRTQLHQQ-UHFFFAOYSA-N copper tin Chemical compound [Cu].[Sn] KUNSUQLRTQLHQQ-UHFFFAOYSA-N 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000012225 czapek media Substances 0.000 description 1
- FWLDHHJLVGRRHD-UHFFFAOYSA-N decyl prop-2-enoate Chemical compound CCCCCCCCCCOC(=O)C=C FWLDHHJLVGRRHD-UHFFFAOYSA-N 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- CRPOUZQWHJYTMS-UHFFFAOYSA-N dialuminum;magnesium;disilicate Chemical compound [Mg+2].[Al+3].[Al+3].[O-][Si]([O-])([O-])[O-].[O-][Si]([O-])([O-])[O-] CRPOUZQWHJYTMS-UHFFFAOYSA-N 0.000 description 1
- 150000001991 dicarboxylic acids Chemical class 0.000 description 1
- 125000004177 diethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- PSLWZOIUBRXAQW-UHFFFAOYSA-M dimethyl(dioctadecyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCCCCCCCCCC[N+](C)(C)CCCCCCCCCCCCCCCCCC PSLWZOIUBRXAQW-UHFFFAOYSA-M 0.000 description 1
- REZZEXDLIUJMMS-UHFFFAOYSA-M dimethyldioctadecylammonium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCCCC[N+](C)(C)CCCCCCCCCCCCCCCCCC REZZEXDLIUJMMS-UHFFFAOYSA-M 0.000 description 1
- 235000019329 dioctyl sodium sulphosuccinate Nutrition 0.000 description 1
- USIUVYZYUHIAEV-UHFFFAOYSA-N diphenyl ether Chemical compound C=1C=CC=CC=1OC1=CC=CC=C1 USIUVYZYUHIAEV-UHFFFAOYSA-N 0.000 description 1
- YDEXUEFDPVHGHE-GGMCWBHBSA-L disodium;(2r)-3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Na+].[Na+].COC1=CC=CC(C[C@H](CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O YDEXUEFDPVHGHE-GGMCWBHBSA-L 0.000 description 1
- GMSCBRSQMRDRCD-UHFFFAOYSA-N dodecyl 2-methylprop-2-enoate Chemical compound CCCCCCCCCCCCOC(=O)C(C)=C GMSCBRSQMRDRCD-UHFFFAOYSA-N 0.000 description 1
- 229940043264 dodecyl sulfate Drugs 0.000 description 1
- SYELZBGXAIXKHU-UHFFFAOYSA-N dodecyldimethylamine N-oxide Chemical class CCCCCCCCCCCC[N+](C)(C)[O-] SYELZBGXAIXKHU-UHFFFAOYSA-N 0.000 description 1
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 description 1
- 235000019414 erythritol Nutrition 0.000 description 1
- 229940009714 erythritol Drugs 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- NHOGGUYTANYCGQ-UHFFFAOYSA-N ethenoxybenzene Chemical compound C=COC1=CC=CC=C1 NHOGGUYTANYCGQ-UHFFFAOYSA-N 0.000 description 1
- AFSIMBWBBOJPJG-UHFFFAOYSA-N ethenyl octadecanoate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC=C AFSIMBWBBOJPJG-UHFFFAOYSA-N 0.000 description 1
- UIWXSTHGICQLQT-UHFFFAOYSA-N ethenyl propanoate Chemical compound CCC(=O)OC=C UIWXSTHGICQLQT-UHFFFAOYSA-N 0.000 description 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 1
- STVZJERGLQHEKB-UHFFFAOYSA-N ethylene glycol dimethacrylate Chemical compound CC(=C)C(=O)OCCOC(=O)C(C)=C STVZJERGLQHEKB-UHFFFAOYSA-N 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 229920002313 fluoropolymer Polymers 0.000 description 1
- 239000004811 fluoropolymer Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- SKCKOFZKJLZSFA-FSIIMWSLSA-N fucitol Chemical compound C[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO SKCKOFZKJLZSFA-FSIIMWSLSA-N 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- FBPFZTCFMRRESA-GUCUJZIJSA-N galactitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-GUCUJZIJSA-N 0.000 description 1
- 235000021255 galacto-oligosaccharides Nutrition 0.000 description 1
- 150000003271 galactooligosaccharides Chemical class 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- DLRVVLDZNNYCBX-CQUJWQHSSA-N gentiobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-CQUJWQHSSA-N 0.000 description 1
- 150000002276 gentiobiuloses Chemical class 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000002314 glycerols Chemical class 0.000 description 1
- VOZRXNHHFUQHIL-UHFFFAOYSA-N glycidyl methacrylate Chemical compound CC(=C)C(=O)OCC1CO1 VOZRXNHHFUQHIL-UHFFFAOYSA-N 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- IUJAMGNYPWYUPM-UHFFFAOYSA-N hentriacontane Chemical compound CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC IUJAMGNYPWYUPM-UHFFFAOYSA-N 0.000 description 1
- PBZROIMXDZTJDF-UHFFFAOYSA-N hepta-1,6-dien-4-one Chemical compound C=CCC(=O)CC=C PBZROIMXDZTJDF-UHFFFAOYSA-N 0.000 description 1
- UBHWBODXJBSFLH-UHFFFAOYSA-N hexadecan-1-ol;octadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCO.CCCCCCCCCCCCCCCCCCO UBHWBODXJBSFLH-UHFFFAOYSA-N 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910001867 inorganic solvent Inorganic materials 0.000 description 1
- 239000003049 inorganic solvent Substances 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000905 isomalt Substances 0.000 description 1
- 235000010439 isomalt Nutrition 0.000 description 1
- HPIGCVXMBGOWTF-UHFFFAOYSA-N isomaltol Natural products CC(=O)C=1OC=CC=1O HPIGCVXMBGOWTF-UHFFFAOYSA-N 0.000 description 1
- DLRVVLDZNNYCBX-RTPHMHGBSA-N isomaltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-RTPHMHGBSA-N 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- PZDOWFGHCNHPQD-OQPGPFOOSA-N kojibiose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](C=O)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O PZDOWFGHCNHPQD-OQPGPFOOSA-N 0.000 description 1
- 239000000832 lactitol Substances 0.000 description 1
- 235000010448 lactitol Nutrition 0.000 description 1
- VQHSOMBJVWLPSR-JVCRWLNRSA-N lactitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O VQHSOMBJVWLPSR-JVCRWLNRSA-N 0.000 description 1
- 229960003451 lactitol Drugs 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- JCQLYHFGKNRPGE-FCVZTGTOSA-N lactulose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 JCQLYHFGKNRPGE-FCVZTGTOSA-N 0.000 description 1
- 229960000511 lactulose Drugs 0.000 description 1
- PFCRQPBOOFTZGQ-UHFFFAOYSA-N lactulose keto form Natural products OCC(=O)C(O)C(C(O)CO)OC1OC(CO)C(O)C(O)C1O PFCRQPBOOFTZGQ-UHFFFAOYSA-N 0.000 description 1
- QIGJYVCQYDKYDW-LCOYTZNXSA-N laminarabiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]1[C@H](O)[C@@H](CO)OC(O)[C@@H]1O QIGJYVCQYDKYDW-LCOYTZNXSA-N 0.000 description 1
- LAPRIVJANDLWOK-UHFFFAOYSA-N laureth-5 Chemical class CCCCCCCCCCCCOCCOCCOCCOCCOCCO LAPRIVJANDLWOK-UHFFFAOYSA-N 0.000 description 1
- PBOSTUDLECTMNL-UHFFFAOYSA-N lauryl acrylate Chemical compound CCCCCCCCCCCCOC(=O)C=C PBOSTUDLECTMNL-UHFFFAOYSA-N 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000010687 lubricating oil Substances 0.000 description 1
- 235000019359 magnesium stearate Nutrition 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- FPYJFEHAWHCUMM-UHFFFAOYSA-N maleic anhydride Chemical compound O=C1OC(=O)C=C1 FPYJFEHAWHCUMM-UHFFFAOYSA-N 0.000 description 1
- VQHSOMBJVWLPSR-WUJBLJFYSA-N maltitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O VQHSOMBJVWLPSR-WUJBLJFYSA-N 0.000 description 1
- 235000010449 maltitol Nutrition 0.000 description 1
- 239000000845 maltitol Substances 0.000 description 1
- 229940035436 maltitol Drugs 0.000 description 1
- 229960003017 maltose monohydrate Drugs 0.000 description 1
- JCQLYHFGKNRPGE-HFZVAGMNSA-N maltulose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JCQLYHFGKNRPGE-HFZVAGMNSA-N 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- FQPSGWSUVKBHSU-UHFFFAOYSA-N methacrylamide Chemical compound CC(=C)C(N)=O FQPSGWSUVKBHSU-UHFFFAOYSA-N 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- LVHBHZANLOWSRM-UHFFFAOYSA-N methylenebutanedioic acid Natural products OC(=O)CC(=C)C(O)=O LVHBHZANLOWSRM-UHFFFAOYSA-N 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- CWQXQMHSOZUFJS-UHFFFAOYSA-N molybdenum disulfide Chemical compound S=[Mo]=S CWQXQMHSOZUFJS-UHFFFAOYSA-N 0.000 description 1
- 229910052982 molybdenum disulfide Inorganic materials 0.000 description 1
- PSZYNBSKGUBXEH-UHFFFAOYSA-M naphthalene-1-sulfonate Chemical compound C1=CC=C2C(S(=O)(=O)[O-])=CC=CC2=C1 PSZYNBSKGUBXEH-UHFFFAOYSA-M 0.000 description 1
- QIGJYVCQYDKYDW-NSYYTRPSSA-N nigerose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](CO)OC(O)[C@@H]1O QIGJYVCQYDKYDW-NSYYTRPSSA-N 0.000 description 1
- 150000002825 nitriles Chemical class 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- SMGTYJPMKXNQFY-UHFFFAOYSA-N octenidine dihydrochloride Chemical compound Cl.Cl.C1=CC(=NCCCCCCCC)C=CN1CCCCCCCCCCN1C=CC(=NCCCCCCCC)C=C1 SMGTYJPMKXNQFY-UHFFFAOYSA-N 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000012168 ouricury wax Substances 0.000 description 1
- RVTZCBVAJQQJTK-UHFFFAOYSA-N oxygen(2-);zirconium(4+) Chemical compound [O-2].[O-2].[Zr+4] RVTZCBVAJQQJTK-UHFFFAOYSA-N 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 229910052625 palygorskite Inorganic materials 0.000 description 1
- 229940094461 penicillium glabrum Drugs 0.000 description 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
- PNJWIWWMYCMZRO-UHFFFAOYSA-N pent‐4‐en‐2‐one Natural products CC(=O)CC=C PNJWIWWMYCMZRO-UHFFFAOYSA-N 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000012169 petroleum derived wax Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 229910052628 phlogopite Inorganic materials 0.000 description 1
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 1
- 150000008104 phosphatidylethanolamines Chemical class 0.000 description 1
- 229960000502 poloxamer Drugs 0.000 description 1
- 229920001983 poloxamer Polymers 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 229920001610 polycaprolactone Polymers 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 235000010958 polyglycerol polyricinoleate Nutrition 0.000 description 1
- 239000003996 polyglycerol polyricinoleate Substances 0.000 description 1
- 229920005862 polyol Polymers 0.000 description 1
- 150000003077 polyols Chemical class 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 description 1
- 235000010483 polyoxyethylene sorbitan monopalmitate Nutrition 0.000 description 1
- 239000000249 polyoxyethylene sorbitan monopalmitate Substances 0.000 description 1
- 235000010989 polyoxyethylene sorbitan monostearate Nutrition 0.000 description 1
- 239000001818 polyoxyethylene sorbitan monostearate Substances 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229950008882 polysorbate Drugs 0.000 description 1
- 229940068977 polysorbate 20 Drugs 0.000 description 1
- 229940101027 polysorbate 40 Drugs 0.000 description 1
- 229940113124 polysorbate 60 Drugs 0.000 description 1
- 229940068968 polysorbate 80 Drugs 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 229940068965 polysorbates Drugs 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 229920002689 polyvinyl acetate Polymers 0.000 description 1
- 239000011118 polyvinyl acetate Substances 0.000 description 1
- 239000001965 potato dextrose agar Substances 0.000 description 1
- 235000008476 powdered milk Nutrition 0.000 description 1
- 229940078492 ppg-17 Drugs 0.000 description 1
- ARENMZZMCSLORU-UHFFFAOYSA-N propan-2-yl naphthalene-1-sulfonate Chemical class C1=CC=C2C(S(=O)(=O)OC(C)C)=CC=CC2=C1 ARENMZZMCSLORU-UHFFFAOYSA-N 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- ARIWANIATODDMH-UHFFFAOYSA-N rac-1-monolauroylglycerol Chemical compound CCCCCCCCCCCC(=O)OCC(O)CO ARIWANIATODDMH-UHFFFAOYSA-N 0.000 description 1
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
- 238000002310 reflectometry Methods 0.000 description 1
- HEBKCHPVOIAQTA-ZXFHETKHSA-N ribitol Chemical compound OC[C@H](O)[C@H](O)[C@H](O)CO HEBKCHPVOIAQTA-ZXFHETKHSA-N 0.000 description 1
- 239000003128 rodenticide Substances 0.000 description 1
- 229940081623 rose bengal Drugs 0.000 description 1
- 229930187593 rose bengal Natural products 0.000 description 1
- STRXNPAVPKGJQR-UHFFFAOYSA-N rose bengal A Natural products O1C(=O)C(C(=CC=C2Cl)Cl)=C2C21C1=CC(I)=C(O)C(I)=C1OC1=C(I)C(O)=C(I)C=C21 STRXNPAVPKGJQR-UHFFFAOYSA-N 0.000 description 1
- OVVGHDNPYGTYIT-BNXXONSGSA-N rutinose Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)O1 OVVGHDNPYGTYIT-BNXXONSGSA-N 0.000 description 1
- 150000003308 rutinuloses Chemical class 0.000 description 1
- 150000003902 salicylic acid esters Chemical class 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 150000004760 silicates Chemical class 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 1
- 229920005552 sodium lignosulfonate Polymers 0.000 description 1
- RYYKJJJTJZKILX-UHFFFAOYSA-M sodium octadecanoate Chemical compound [Na+].CCCCCCCCCCCCCCCCCC([O-])=O RYYKJJJTJZKILX-UHFFFAOYSA-M 0.000 description 1
- FGDMJJQHQDFUCP-UHFFFAOYSA-M sodium;2-propan-2-ylnaphthalene-1-sulfonate Chemical compound [Na+].C1=CC=CC2=C(S([O-])(=O)=O)C(C(C)C)=CC=C21 FGDMJJQHQDFUCP-UHFFFAOYSA-M 0.000 description 1
- 238000010563 solid-state fermentation Methods 0.000 description 1
- PZDOWFGHCNHPQD-VNNZMYODSA-N sophorose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](C=O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O PZDOWFGHCNHPQD-VNNZMYODSA-N 0.000 description 1
- 239000008347 soybean phospholipid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000012177 spermaceti Substances 0.000 description 1
- 229940084106 spermaceti Drugs 0.000 description 1
- 235000015096 spirit Nutrition 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 229920001909 styrene-acrylic polymer Polymers 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- OULAJFUGPPVRBK-UHFFFAOYSA-N tetratriacontyl alcohol Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCO OULAJFUGPPVRBK-UHFFFAOYSA-N 0.000 description 1
- AOBORMOPSGHCAX-DGHZZKTQSA-N tocofersolan Chemical compound OCCOC(=O)CCC(=O)OC1=C(C)C(C)=C2O[C@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C AOBORMOPSGHCAX-DGHZZKTQSA-N 0.000 description 1
- 229960000984 tocofersolan Drugs 0.000 description 1
- LBLYYCQCTBFVLH-UHFFFAOYSA-M toluenesulfonate group Chemical class C=1(C(=CC=CC1)S(=O)(=O)[O-])C LBLYYCQCTBFVLH-UHFFFAOYSA-M 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- UBOXGVDOUJQMTN-UHFFFAOYSA-N trichloroethylene Natural products ClCC(Cl)Cl UBOXGVDOUJQMTN-UHFFFAOYSA-N 0.000 description 1
- ITRNXVSDJBHYNJ-UHFFFAOYSA-N tungsten disulfide Chemical compound S=[W]=S ITRNXVSDJBHYNJ-UHFFFAOYSA-N 0.000 description 1
- RULSWEULPANCDV-PIXUTMIVSA-N turanose Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](C(=O)CO)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RULSWEULPANCDV-PIXUTMIVSA-N 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 208000007089 vaccinia Diseases 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229920001567 vinyl ester resin Polymers 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- FUSUHKVFWTUUBE-UHFFFAOYSA-N vinyl methyl ketone Natural products CC(=O)C=C FUSUHKVFWTUUBE-UHFFFAOYSA-N 0.000 description 1
- 239000012873 virucide Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- OXQKEKGBFMQTML-KVTDHHQDSA-N volemitol Chemical compound OC[C@@H](O)[C@@H](O)C(O)[C@H](O)[C@H](O)CO OXQKEKGBFMQTML-KVTDHHQDSA-N 0.000 description 1
- GDJZZWYLFXAGFH-UHFFFAOYSA-M xylenesulfonate group Chemical group C1(C(C=CC=C1)C)(C)S(=O)(=O)[O-] GDJZZWYLFXAGFH-UHFFFAOYSA-M 0.000 description 1
- 229910001928 zirconium oxide Inorganic materials 0.000 description 1
- 239000004711 α-olefin Substances 0.000 description 1
- 235000004835 α-tocopherol Nutrition 0.000 description 1
- 239000002076 α-tocopherol Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/36—Penicillium
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/80—Penicillium
Definitions
- the present disclosure contains references to biological material deposited under the terms of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure at the Agricultural Research Service Culture Collection, 1815 North University Street, Peoria, Ill. 61604, U.S.A.
- the present disclosure relates to isolated fungal strains and new fungal strains derived therefrom.
- Penicillium is capable of solubilizing phosphate. See, e.g., U.S. Pat. Nos. 5,026,417; 5,484,464; 7,241,588; 8,278,247 and 9,101,088; U.S. Patent Publication Nos. 2014/014909.
- a first aspect of the present disclosure is an isolated strain of Penicillium bilaiae selected from the group consisting of the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67154 ( P. bilaiae NRRL 67154), the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67155 ( P. bilaiae NRRL 67155), the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67156 ( P. bilaiae NRRL 67156), the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67157 ( P.
- bilaiae NRRL 67157 the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67158 ( P. bilaiae NRRL 67158), or the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67159 ( P. bilaiae NRRL 67159).
- a second aspect of the present disclosure is a biologically pure culture of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159.
- a third aspect of the present disclosure is an inoculant composition
- P. bilaiae NRRL B-67154 P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159.
- the inoculant composition comprises one or more stabilizing compounds, one or more pesticides, one or more lipo-chitooligosaccharides, one or more chitooligosaccharides, one or more chitinous compounds, one or more flavonoids, and/or one or more additional microorganisms.
- a fourth aspect of the present disclosure is a kit comprising an inoculant composition comprising P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 and a container housing said inoculant composition.
- a fifth aspect of the present disclosure is a method of increasing the amount/concentration of soluble phosphate in a medium that contains insoluble phosphate, said method comprising applying P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 to said medium in an amount/concentration effective to solubilize at least a portion of the insoluble phosphate therein.
- a sixth aspect of the present disclosure is a method of increasing the amount/concentration of soluble phosphate in a medium that contains insoluble phosphate, said method comprising introducing P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 into said medium in an amount/concentration effective to solubilize at least a portion of the insoluble phosphate therein.
- An eighth aspect of the present disclosure is a method of increasing the amount/concentration of soluble phosphate in a medium that contains insoluble phosphate, said method comprising applying a biologically pure culture of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159 to said medium in an amount/concentration effective to solubilize at least a portion of the insoluble phosphate therein.
- a ninth aspect of the present disclosure is a method of increasing the amount/concentration of soluble phosphate in a medium that contains insoluble phosphate, said method comprising introducing the biologically pure culture of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159 into said medium in an amount/concentration effective to solubilize at least a portion of the insoluble phosphate therein.
- a tenth aspect of the present disclosure is a method of increasing the amount/concentration of soluble phosphate in a medium that contains insoluble phosphate, said method comprising applying an inoculant composition comprising P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 to said medium in an amount/concentration effective to solubilize at least a portion of the insoluble phosphate therein.
- An eleventh aspect of the present disclosure is a method of increasing the amount/concentration of soluble phosphate in a medium that contains insoluble phosphate, said method comprising introducing an inoculant composition comprising P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 into said medium in an amount/concentration effective to solubilize at least a portion of the insoluble phosphate therein.
- acaricide and “acaricidal” refer to an agent or combination of agents the application of which is toxic to an acarid (i.e., kills an acarid, inhibits the growth of an acarid and/or inhibits the reproduction of an acarid).
- aqueous refers to a composition that contains more than a trace amount of water (i.e., more than 0.5% water by weight, based upon the total weight of the composition).
- biologically pure culture refers to a microbial culture that is free or essentially free of biological contamination and that has genetic uniformity such that different subcultures taken therefrom will exhibit identical or substantially identical genotyopes and phenotypes.
- the biologically pure culture is 100% pure (i.e., all subcultures taken therefrom exhibit identical genotypes and phenotypes).
- the biologically pure culture is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 99.5, 99.6, 99.7, 99.8, or 99.9% pure (i.e., at least 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 99.5, 99.6, 99.7, 99.8, or 99.9% of the subcultures taken therefrom exhibit identical genotypes and phenotypes).
- BRADY is to be interpreted as a shorthand substitute for the phrase “ Bradyrhizobium elkanii SEMIA 501, Bradyrhizobium elkanii SEMIA 587, Bradyrhizobium elkanii SEMIA 5019, Bradyrhizobium japonicum NRRL B-50586 (also deposited as NRRL B-59565), Bradyrhizobium japonicum NRRL B-50587 (also deposited as NRRL B-59566), Bradyrhizobium japonicum NRRL B-50588 (also deposited as NRRL B-59567), Bradyrhizobium japonicum NRRL B-50589 (also deposited as NRRL B-59568), Bradyrhizobium japonicum NRRL B-50590 (also deposited as NRRL B-59569), Bradyrhizobium japonicum NRRL B-50591 (also deposited as NRRL B-59
- colony forming unit and “cfu” refer to a microbial cell/spore capable of propagating on or in a suitable growth medium or substrate when conditions (e.g., temperature, moisture, nutrient availability, pH, etc.) are favorable for germination and/or microbial growth.
- conditions e.g., temperature, moisture, nutrient availability, pH, etc.
- the term “consists essentially of,” when used in reference to inoculant compositions and methods of the present disclosure, means that the compositions/methods may contain additional components/steps so long as the additional components/steps do not materially alter the composition/method.
- the term “materially alter,” as applied to a composition/method of the present disclosure, refers to an increase or decrease in the effectiveness of the composition/method of at least 20%.
- a component added to an inoculant composition of the present disclosure may be deemed to “materially alter” the composition if it increases or decreases the composition's ability to solubilize phosphate by at least 20%.
- diazotroph refers to an organism capable of converting atmospheric nitrogen (N 2 ) into a form that may be utilized by a plant or plant part (e.g., ammonia (NH 3 ), ammonium (NH 4 +), etc.).
- the term “dispersant” refers to an agent or combination of agents the application of which reduces the cohesiveness of like particles, the surface tension of a liquid, the interfacial tension between two liquids and/or the interfacial tension between or a liquid and a solid.
- the terms “effective amount,” “effective concentration” and “effective amount/concentration” refer to an amount or concentration that is sufficient to cause a desired effect (e.g., enhanced phosphate availability).
- the absolute value of the amount/concentration that is sufficient to cause the desired effect may be affected by factors such as the type and magnitude of effect desired, the type, size and volume of material to which the inoculant composition will be applied, the type(s) of microorganisms in the composition, the number of microorganisms in the composition, the stability of the microorganism(s) in the inoculant composition and the storage conditions (e.g., temperature, relative humidity, duration). Those skilled in the art will understand how to select an effective amount/concentration using routine dose-response experiments.
- the term “enhanced dispersion” refers to an improvement in one or more characteristics of microbial dispersion as compared to one or more controls (e.g., a control composition that is identical to an inoculant composition of the present disclosure except that it lacks one or more of the components found in the inoculant composition of the present disclosure).
- exemplary microbial dispersion characteristics include, but are not limited to, the percentage of microbes that exist as single cells/spores when the inoculant composition is diluted in water.
- An inoculant composition that improves one or more microbial dispersion characteristics of the microorganism(s) contained therein as compared to a control composition e.g., a control composition that is identical to the inoculant composition except that it lacks one or more of the components found in the inoculant composition
- a control composition e.g., a control composition that is identical to the inoculant composition except that it lacks one or more of the components found in the inoculant composition
- a control composition provides enhanced dispersion and can be referred to as a “readily dispersable inoculant composition.”
- the terms “enhanced stability” and “enhanced microbial stability” refer to an improvement in one or more characteristics of microbial stability as compared to one or more controls (e.g., a control composition that is identical to an inoculant composition of the present disclosure except that it lacks one or more of the components found in the inoculant composition of the present disclosure).
- exemplary microbial stability characteristics include, but are not limited to, the ability to germinate and/or propagate after being stored for a defined period of time and the ability to cause a desired effect (e.g., increased phosphate solubility) after being stored for a defined period of time.
- An inoculant composition that improves one or more microbial stability characteristics of the microorganism(s) contained therein as compared to a control composition e.g., a control composition that is identical to the inoculant composition except that it lacks one or more of the components found in the inoculant composition
- the terms “enhanced survival” and “enhanced microbial survival” refer to an improvement in the survival rate of one or more microorganisms in an inoculant composition as compared to one or more microorganisms in a control composition (e.g., a control composition that is identical to an inoculant composition of the present disclosure except that it lacks one or more of the components found in the inoculant composition of the present disclosure).
- An inoculant composition that improves the survival rate of one or more of the microorganisms contained therein as compared to a control composition provides enhanced survival and can be referred to as a stable inoculant composition.
- fungicide and “fungicidal” refer to an agent or combination of agents the application of which is toxic to a fungus (i.e., kills a fungus, inhibits the growth of a fungus and/or inhibits the reproduction of a fungus).
- the term “fulvic acid” encompasses pure fulvic acids and fulvic acid salts (fulvates).
- fulvic acids include ammonium fulvate, boron fulvate, potassium fulvate, sodium fulvate, etc.
- the fulvic acid comprises, consists essentially of or consists MDL Number MFCD09838488 (CAS Number 479-66-3).
- the terms “herbicide” and “herbicidal” refer to an agent or combination of agents the application of which is toxic to a weed (i.e., kills a weed, inhibits the growth of a weed and/or inhibits the reproduction of a weed).
- the term “humic acid” encompasses pure humic acids and humic acid salts (humates).
- humic acids include ammonium humate, boron humate, potassium humate, sodium humate, etc.
- the humic acid comprises, consists essentially of or consists of one or more of MDL Number MFCD00147177 (CAS Number 1415-93-6), MDL Number MFCD00135560 (CAS Number 68131-04-4), MDL Number MFCS22495372 (CAS Number 68514-28-3), CAS Number 93924-35-7 and CAS Number 308067-45-0.
- inoculant composition and “inoculum” refer to a composition comprising microbial cells and/or spores, said cells/spores being capable of propagating/germinating on or in a suitable growth medium or substrate when conditions (e.g., temperature, moisture, nutrient availability, pH, etc.) are favorable for germination and/or microbial growth.
- conditions e.g., temperature, moisture, nutrient availability, pH, etc.
- insecticide and “insecticidal” refer to an agent or combination of agents the application of which is toxic to an insect (i.e., kills an insect, inhibits the growth of an insect and/or inhibits the reproduction of an insect).
- isolated microbial strain refers to a microbe that has been removed from the environment in which it is normally found.
- the term “isomer” includes all stereoisomers of the compounds and/or molecules to which it refers, including enantiomers and diastereomers, as well as all conformers, roatmers and tautomers, unless otherwise indicated.
- Compounds and/or molecules disclosed herein include all enantiomers in either substantially pure levorotatory or dextrorotatory form, or in a racemic mixture, or in any ratio of enantiomers. Where embodiments disclose a (D)-enantiomer, that embodiment also includes the (L)-enantiomer; where embodiments disclose a (L)-enantiomer, that embodiment also includes the (D)-enantiomer.
- embodiments disclose a (+)-enantiomer, that embodiment also includes the ( ⁇ )-enantiomer; where embodiments disclose a ( ⁇ )-enantiomer, that embodiment also includes the (+)-enantiomer.
- embodiments disclose a (S)-enantiomer, that embodiment also includes the (R)-enantiomer; where embodiments disclose a (R)-enantiomer, that embodiment also includes the (S)-enantiomer.
- Embodiments are intended to include any diastereomers of the compounds and/or molecules referred to herein in diastereomerically pure form and in the form of mixtures in all ratios. Unless stereochemistry is explicitly indicated in a chemical structure or chemical name, the chemical structure or chemical name is intended to embrace all possible stereoisomers, conformers, rotamers and tautomers of compounds and/or molecules depicted.
- modified microbial strain refers to a microbial strain that is modified from a strain isolated from nature. Modified microbial strains may be produced by any suitable method(s), including, but not limited to, chemical or other form of induced mutation to a polynucleotide within any genome within the strain; the insertion or deletion of one or more nucleotides within any genome within the strain, or combinations thereof; an inversion of at least one segment of DNA within any genome within the strain; a rearrangement of any genome within the strain; generalized or specific transduction of homozygous or heterozygous polynucleotide segments into any genome within the strain; introduction of one or more phage into any genome of the strain; transformation of any strain resulting in the introduction into the strain of stably replicating autonomous extrachromosomal DNA; any change to any genome or to the total DNA composition within the strain isolated from nature as a result of conjugation with any different microbial strain; and any combination of the foregoing.
- modified microbial strains includes a strain with (a) one of more heterologous nucleotide sequences, (b) one or more non-naturally occurring copies of a nucleotide sequence isolated from nature (i.e., additional copies of a gene that naturally occurs in the microbial strain from which the modified microbial strain was derived), (c) a lack of one or more nucleotide sequences that would otherwise be present in the natural reference strain by for example deleting nucleotide sequence, and (d) added extrachromosomal DNA.
- modified microbial strains comprise a combination of two or more nucleotide sequences (e.g., two or more naturally occurring genes that do not naturally occur in the same microbial strain) or comprise a nucleotide sequence isolated from nature at a locus that is different from the natural locus.
- nematicide and “nematicidal” refer to an agent or combination of agents the application of which is toxic to a nematode (i.e., kills a nematode, inhibits the growth of a nematode and/or inhibits the reproduction of a nematode).
- nitrogen fixing organism refers to an organism capable of converting atmospheric nitrogen (N 2 ) into a form that may be utilized by a plant or plant part (e.g., ammonia (NH 3 ), ammonium (NH 4 +), etc.).
- non-aqueous refers to a composition that comprises no more than a trace amount of water (i.e., no more than 0.5% water by weight, based upon the total weight of the composition).
- Penicillium bilaiae is intended to include all iterations of the species name, such as “ Penicillium bilaji ” and “ Penicillium bilaii.”
- percent identity refers to the relatedness of two or more nucleotide or amino acid sequences, which may be calculated by (i) comparing two optimally aligned sequences over a window of comparison, (ii) determining the number of positions at which the identical nucleic acid base (for nucleotide sequences) or amino acid residue (for proteins) occurs in both sequences to yield the number of matched positions, (iii) dividing the number of matched positions by the total number of positions in the window of comparison, and then (iv) multiplying this quotient by 100% to yield the percent identity.
- the percent identity is being calculated in relation to a reference sequence without a particular comparison window being specified, then the percent identity is determined by dividing the number of matched positions over the region of alignment by the total length of the reference sequence. Accordingly, for purposes of the present invention, when two sequences (query and subject) are optimally aligned (with allowance for gaps in their alignment), the “percent identity” for the query sequence is equal to the number of identical positions between the two sequences divided by the total number of positions in the query sequence over its length (or a comparison window), which is then multiplied by 100%.
- pesticide and “pesticidal” refer to agents or combinations of agents the application of which is toxic to a pest (i.e., kills a pest, inhibits the growth of a pest and/or inhibits the reproduction of a pest).
- pesticides include acaricides, fungicides, herbicides, insecticides, nematicides, rodenticides, virucides, gastropodicides, etc.
- phosphate-solubilizing microorganism refers to a microorganism capable of converting insoluble phosphate into a soluble form of phosphate.
- the term “progeny” refers to the descendent(s) of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 and encompasses both immediate offspring of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 and any decendants thereof.
- spore and “microbial spore” refer to a microorganism in its dormant, protected state.
- stabilizing compound refers to an agent or combination of agents the application of which enhances the stability and/or survival of one or more microorganisms in an inoculant composition.
- stable refers to an inoculant composition in which microorganisms exhibit enhanced stability and/or enhanced survival.
- an inoculant composition may be labeled “stable” if it improves the survival rate and/or at least one microbial stability characteristic of at least one microorganism contained therein.
- the term “survival rate” refers to the percentage of microbial cell/spore that are viable (i.e., capable of propagating on or in a suitable growth medium or substrate when conditions (e.g., temperature, moisture, nutrient availability, pH, etc.) are favorable for germination and/or microbial growth) at a given period of time.
- the present disclosure provides isolated fungal strains capable of converting insoluble phosphate into a soluble form of phosphate.
- the isolated fungal strain is P. bilaiae NRRL 67154 , P. bilaiae NRRL 67155 , P. bilaiae NRRL 67156 , P. bilaiae NRRL 67157 , P. bilaiae NRRL 67158, or P. bilaiae NRRL 67159.
- the present disclosure extends to the progeny of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 and to modified microbial strains derived from P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159.
- Progeny of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 may be produced using any suitable method(s), including, but not limited to, protoplast fusion, traditional breeding programs (e.g., backcrossing, inbredding, etc.) and combinations thereof.
- the progeny is born of a cross between two of P. bilaiae NRRL B-67154 , P.
- the progeny is born of a cross between one of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and P. bilaiae NRRL B-67159.
- the progeny is born of a cross between one of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and P.
- the progeny is born of a cross between one of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and P. bilaiae NRRL B-67159 and a fungal strain incapable of converting insoluble phosphate into a soluble form of phosphate.
- Modified microbial strains derived from P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 may be produced using suitable method(s), including, but not limited to, chemical or other form of induced mutation to a polynucleotide within any genome within P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P.
- bilaiae NRRL B-67156 P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159; the insertion or deletion of one or more nucleotides within any genome within P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, or P.
- bilaiae NRRL B-67159 or combinations thereof; an inversion of at least one segment of DNA within any genome within P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159; a rearrangement of any genome within P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P.
- bilaiae NRRL B-67156 P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159; generalized or specific transduction of homozygous or heterozygous polynucleotide segments into any genome within P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, or P.
- bilaiae NRRL B-67159 introduction of one or more phage into any genome of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159; transformation of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P.
- bilaiae NRRL B-67157 P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159 resulting in the introduction into P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159 of stably replicating autonomous extrachromosomal DNA; any change to any genome or to the total DNA composition within P.
- bilaiae NRRL B-67154 P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159 as a result of conjugation with any different microbial strain; and any combination of the foregoing.
- Fungal strains of the present disclosure may be cultured using any suitable method(s), including, but not limited to, liquid-state fermentation and solid-state fermentation. See, generally, Cunningham et al., CAN. J. BOT. 68:2270 (1990); Friesen et al., APPL. MICROBIOL. BIOTECH. 68:397 (2005).
- Fungal strains of the present disclosure may be harvested during any suitable growth phase.
- the fungal strain is allowed to reach the stationary growth phase prior to harvesting.
- the fungal strain is harvested as vegetative cells.
- the fungal strain is harvested as spores.
- Fungal strains of the present disclosure may be harvested and/or concentrated using any suitable method(s), including, but not limited to, centrifugation (e.g., density gradient centrifugation, disc stack centrifugation, tubular bowl centrifugation), coagulation, decanting, felt bed collection, filtration (e.g., drum filtration, sieving, ultrafiltration), flocculation, impaction and trapping (e.g., cyclone spore trapping, liquid impingement).
- centrifugation e.g., density gradient centrifugation, disc stack centrifugation, tubular bowl centrifugation
- coagulation coagulation
- decanting felt bed collection
- filtration e.g., drum filtration, sieving, ultrafiltration
- flocculation e.g., cyclone spore trapping, liquid impingement
- the present disclosure also provides biologically pure cultures of the fungal strains described herein.
- at least 95, 96, 97, 98, 99, 99.5, 99.6, 99.7, 99.8, or 99.9% of subcultures taken from the culture exhibit a genotype that is at least 95, 96, 97, 98, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.55%, 99.6%, 99.65%, 99.7%, 99.75%, 99.8%, 99.85%, 99.9%, 99.95%, or 100% identical to that of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P.
- the culture is a biologically pure culture of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159.
- cultures of the present invention may comprise vegetative cells and/or dormant spores.
- at least 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98, 99% or more of the microbes in a culture of the present disclosure are present as vegetative cells.
- at least 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98, 99% or more of the microbes in a culture of the present disclosure are present as spores.
- Fungal strains and cultures of the present disclosure may be formulated into any suitable type of composition.
- the present disclosure provides inoculant compositions comprising one or more fungal strains of the present disclosure in a carrier.
- Fungal strains and cultures of the present disclosure may be incorporated into any suitable inoculant composition, including, but not limited to, inoculant compositions as described in International Patent Application Nos. PCT/US2016/050529 (filed Sep. 7, 2016); PCT/US2016/050647 (filed Sep. 8, 2016); PCT/US2016/067714 (filed Dec. 20, 2016); and PCT/US2016/067745 (filed Dec. 20, 2016); U.S Provisional Patent Nos. 62/343,217 (filed May 31, 2016); 62/347,773 (filed Jun. 9, 2016); 62/347,785 (filed Jun. 9, 2016); 62/347,794 (filed Jun. 9, 2016); 62/347,805 (filed Jun. 9, 2016); and 62/436,529 (filed Dec. 20, 2016).
- PCT/US2016/050529 filed Sep. 7, 2016
- PCT/US2016/050647 filed Sep. 8, 2016
- Fungal strains of the present disclosure may be incorporated into inoculant compositions in any suitable amount/concentration.
- the absolute value of the amount/concentration that is/are sufficient to cause the desired effect(s) may be affected by factors such as the type, size and volume of material to which the composition will be applied and storage conditions (e.g., temperature, relative humidity, duration). Those skilled in the art will understand how to select an effective amount/concentration using routine dose-response experiments.
- inoculant compositions of the present disclosure comprise one or more fungal strains of the present disclosure in an amount ranging from about 1 ⁇ 10 1 to about 1 ⁇ 10 15 colony-forming units (cfu) per gram and/or milliliter of inoculant composition.
- inoculant compositions of the present disclosure may comprise about 1 ⁇ 10 1 , 1 ⁇ 10 2 , 1 ⁇ 10 3 , 1 ⁇ 10 4 , 1 ⁇ 10 5 , 1 ⁇ 10 6 , 1 ⁇ 10 7 , 1 ⁇ 10 8 , 1 ⁇ 10 9 , 1 ⁇ 10 10 , 1 ⁇ 10 11 , 1 ⁇ 10 12 , 1 ⁇ 10 13 , 1 ⁇ 10 14 , 1 ⁇ 10 15 or more cfu of P.
- bilaiae NRRL B-67154 P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 cells/spores per gram and/or milliliter of inoculant composition.
- inoculant compositions of the present disclosure comprise at least 1 ⁇ 10 4 , 1 ⁇ 10 5 , 1 ⁇ 10 6 , 1 ⁇ 10 7 , 1 ⁇ 10 8 , 1 ⁇ 10 9 , 1 ⁇ 10 10 , 1 ⁇ 10 11 , 1 ⁇ 10 12 cfu of one or more fungal strains of the present disclosure per gram and/or milliliter of inoculant composition.
- cells/spores of one or more fungal strains of the present disclosure comprises about 0.1 to about 95% (by weight) of the inoculant composition.
- P. bilaiae NRRL B-67154 P. bilaiae NRRL B-67155
- P. bilaiae NRRL B-67156 P. bilaiae NRRL B-67157
- P. bilaiae NRRL B-67158 P.
- bilaiae NRRL B-67159 cells/spores may comprise about 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of the inoculant composition.
- P. bilaiae NRRL B-67154 P.
- P. bilaiae NRRL B-67155 comprise about 1 to about 25%, about 5 to about 20%, about 5 to about 15%, about 5 to about 10% or about 8 to about 12% (by weight) of the inoculant composition.
- P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and P. bilaiae NRRL B-67159 are capable of converting insoluble phosphate into a soluble form of phosphate.
- the aforementioned utility is not limited to P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and P. bilaiae NRRL B-67159.
- P. bilaiae NRRL B-67158 and/or P. bilaiae NRRL B-67159 and modified microbial strains derived from P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 may also be capable of solubilizing phosphate. Those skilled in the art will understand how to determine whether a given strain is capable of solubilizing phosphate using routine experiments.
- inoculant compositions of the present disclosure comprise one or more phosphate-solubilizing progeny of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P.
- bilaiae NRRL B-67159 (e.g., an elite phosphate-solubilizing inbred strain having a whole genome sequence that is at 99, 99.1, 99.2, 99.3, 99.4, 99.5, 99.6, 99.7, 99.8 or 99.9% identical to that of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P.
- P. bilaiae NRRL B-67159 e.g., an elite phosphate-solubilizing inbred strain having a whole genome sequence that is at 99, 99.1, 99.2, 99.3, 99.4, 99.5, 99.6, 99.7, 99.8 or 99.9% identical to that of P. bilaia
- bilaiae NRRL B-67159 and/or one or more phosphate-solubilizing modified microbial strains derived from P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P.
- bilaiae NRRL B-67159 (e.g., a modified microbial strain that solubilizes phosphate and has a whole genome sequence that is at least 99, 99.1, 99.2, 99.3, 99.4, 99.5, 99.6, 99.7, 99.8 or 99.9% identical to that of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159).
- P. bilaiae NRRL B-67154 P. bilaiae NRRL B-67155
- P. bilaiae NRRL B-67156 P. bilaiae NRRL B-67157
- inoculant compositions of the present disclosure comprise one or more fungal strains of the present disclosure in an effective amount/concentration for increasing the amount/concentration of soluble phosphate when the inoculant composition is introduced into a medium that comprises insoluble phosphate.
- inoculant compositions of the present disclosure comprise one or more fungal strains of the present disclosure in an amount/concentration sufficient to increase the amount/concentration of soluble phosphate by at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 225, 250% or more when the inoculant composition is introduced into the medium.
- inoculant compositions of the present disclosure comprise cells/spores of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P.
- bilaiae NRRL B-67156 P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 in an amount/concentration effective to increase the amount/concentration of soluble phosphate in a medium by at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 225 or 250% when the inoculant composition is introduced into the medium.
- Inoculant compositions of the present disclosure may comprise any suitable carrier(s). Selection of appropriate carrier materials will depend on the intended application(s) and the microorganism(s) present in the inoculant composition.
- the carrier material(s) will be selected to provide an inoculant composition in the form of a liquid, gel, slurry, or solid.
- the carrier will consist essentially of or consist of one or more stabilizing compounds.
- the inoculant composition comprises one or more solid carriers.
- the inoculant composition comprises one or more powders (e.g., wettable powders) and/or granules.
- solid carriers include clays (e.g., attapulgite clays, montmorillonite clay, etc.), peat-based powders and granules, freeze-dried powders, spray-dried powders, spray-freeze-dried powders and combinations thereof.
- the inoculant composition comprises one or more liquid and/or gel carriers. According to some embodiments, the inoculant composition comprises one or more non-aqueous solvents. According to some embodiments, the inoculant composition comprises one or more aqueous solvents (e.g., water).
- aqueous solvents e.g., water
- an aqueous solvent such as water
- a co-solvent such as ethyl lactate, methyl soyate/ethyl lactate co-solvent blends (e.g., STEPOSOLTM, Stepan), isopropanol, acetone, 1,2-propanediol, n-alkylpyrrolidones (e.g., AGSOLEXTM wetting agents; Ashland, Inc., Covington, Ky.), petroleum based-oils (e.g., AROMATICTM and SOLVESSOTM fluids; ExxonMobil Chemical Company, Spring, Tex.), isoparrafinic hyydrocarbons (e.g., ISOPARTM fluids; ExxonMobil Chemical Company, Spring, Tex.), cycloparaffinic hydrocarbons (e.g., NAPPARTM 6; ExxonMobil Chemical Company, Spring, Tex.), mineral spirits (
- the inoculant composition comprises one or more inorganic solvents, such as decane, dodecane, hexylether and nonane.
- the inoculant composition comprises one or more organic solvents, such as acetone, dichloromethane, ethanol, hexane, methanol, propan-2-ol and trichloroethylene.
- liquid/gel carriers include oils (e.g., mineral oil, olive oil, peanut oil, soybean oil, sunflower oil), polyethylene glycols (e.g., PEG 200, PEG 300, PEG 400, etc.), propylene glycols (e.g., PPG-9, PPG-10, PPG-17, PPG-20, PPG-26, etc.), ethoxylated alcohols (e.g., TOMADOL® (Air Products and Chemicals, Inc., Allentown, Pa.), TERGITOLTM 15-S surfactants such as TERGITOLTM15-S-9 (The Dow Chemical Company, Midland, Mich.), etc.), isoparrafinic hyydrocarbons (e.g., ISOPARTM, ISOPARTM L, ISOPARTM M, ISOPARTM V; ExxonMobil Chemical Company, Spring, Tex.), pentadecane, polysorbates (e.g. polysorbate 20, polysorbate 40, polysorbate 60, polysorbate 80, etc.),
- Inoculant compositions of the present disclosure may comprise any suitable stabilizing compound(s), including, but not limited to, maltodextrins, monosaccharides, disaccharides, oligosaccharides, sugar alcohols, humic acids, fulvic acids, malt extracts, peat extracts, betaines, prolines, sarcosines, peptones, skim milks, oxidation control components, hygroscopic polymers and UV protectants.
- suitable stabilizing compound(s) including, but not limited to, maltodextrins, monosaccharides, disaccharides, oligosaccharides, sugar alcohols, humic acids, fulvic acids, malt extracts, peat extracts, betaines, prolines, sarcosines, peptones, skim milks, oxidation control components, hygroscopic polymers and UV protectants.
- the inoculant composition comprises one or more maltodextrins (e.g., one or more maltodextrins having a dextrose equivalent value (DEV) of about 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25).
- the inoculant composition comprises one or more maltodextrins having a DEV of about 5 to about 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19 or 20, about 10 to about 11, 12, 14, 15, 16, 17, 18, 19 or 20, or about 15 to about 16, 17, 18, 19 or 20.
- the inoculant composition comprises a combination of maltodextrins having a DEV of about 5 to about 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19 or 20, about 10 to about 11, 12, 14, 15, 16, 17, 18, 19 or 20, or about 15 to about 16, 17, 18, 19 or 20.
- the inoculant composition comprises one or more monosaccharides (e.g., allose, altrose, arabinose, fructose, galactose, glucose, gulose, iodose, lyxose, mannose, ribose, talose, threose and/or xylose).
- the inoculant composition comprises gluscose.
- the inoculant composition does not comprise glucose.
- the inoculant composition comprises one or more disaccharides (e.g., cellobiose, chitobiose, gentiobiose, gentiobiulose, isomaltose, kojibiose, lactose, lactulose, laminaribiose, maltose (e.g., maltose monohydrate, anhydrous maltose), maltulose, mannobiose, melibiose, melibiulose, nigerose, palatinose, rutinose, rutinulose, sophorose, sucrose, trehalose, turanose and/or xylobiose).
- disaccharides e.g., cellobiose, chitobiose, gentiobiose, gentiobiulose, isomaltose, kojibiose, lactose, lactulose, laminaribiose, maltose (e.
- the inoculant composition comprises maltose. According to some embodiments, the inoculant composition does not comprise maltose. According to some embodiments, the inoculant composition comprises trehalose. According to some embodiments, the inoculant composition does not comprise trehalose.
- the inoculant composition comprises one or more oligosaccharides (e.g., fructo-oligosaccharides, galacto-oligosaccharides, mannon-oligosaccharides and/or raffinose).
- oligosaccharides e.g., fructo-oligosaccharides, galacto-oligosaccharides, mannon-oligosaccharides and/or raffinose.
- the inoculant composition comprises one or more sugar alcohols (e.g., arabitol, erythritol, fucitol, galactitol, glycerol, iditol, inositol, isomalt, lactitol, maltitol, maltotetraitol, maltotriitol, mannitol, polyglycitol, ribitol, sorbitol, threitol, volemitol and/or xylitol).
- sugar alcohols e.g., arabitol, erythritol, fucitol, galactitol, glycerol, iditol, inositol, isomalt, lactitol, maltitol, maltotetraitol, maltotriitol, mannitol, polyglycitol, ribitol,
- the inoculant composition comprises one or more humic acids (e.g., one or more leonardite humic acids, lignite humic acids, peat humic acids and water-extracted humic acids).
- the inoculant composition comprises ammonium humate, boron humate, potassium humate and/or sodium humate.
- one or more of ammonium humate, boron humate, potassium humate and sodium humate is/are excluded from the inoculant composition.
- Nonlimiting examples of humic acids that may be useful in embodiments of the present disclosure include MDL Number MFCD00147177 (CAS Number 1415-93-6), MDL Number MFCD00135560 (CAS Number 68131-04-4), MDL Number MFCS22495372 (CAS Number 68514-28-3), CAS Number 93924-35-7, and CAS Number 308067-45-0.
- the inoculant composition comprises one or more fulvic acids (e.g., one or more leonardite fulvic acids, lignite fulvic acids, peat fulvic acids and/or water-extracted fulvic acids).
- the inoculant composition comprises ammonium fulvate, boron fulvate, potassium fulvate and/or sodium fulvate.
- one or more of ammonium fulvate, boron fulvate, potassium fulvate and sodium fulvate is/are excluded from inoculant compositions of the present disclosure.
- fulvic acids that may be useful in embodiments of the present disclosure include MDL Number MFCD09838488 (CAS Number 479-66-3).
- the inoculant composition comprises one or more betaines (e.g., trimethylglycine).
- the inoculant composition comprises one or more peptones (e.g., bacterial peptones, meat peptones, milk peptones, vegetable peptones and yeast peptones).
- peptones e.g., bacterial peptones, meat peptones, milk peptones, vegetable peptones and yeast peptones.
- the inoculant composition comprises one or more oxidation control components (e.g., one or more antioxidants and/or oxygen scavengers).
- the inoculant composition comprises one or more oxygen scavengers, such as ascrobic acid, ascorbate salts, catechol and/or sodium hydrogen carbonate.
- the inoculant composition comprises one or more antioxidants, such as ascorbic acid, ascorbyl palmitate, ascorbyl stearate, calcium ascorbate, carotenoids, lipoic acid, phenolic compounds (e.g., flavonoids, flavones, flavonols), potassium ascorbate, sodium ascorbate, thiols (e.g., glutathione, lipoic acid, N-acetyl cysteine), tocopherols, tocotrienols, ubiquinone and/or uric acid.
- antioxidants such as ascorbic acid, ascorbyl palmitate, ascorbyl stearate, calcium ascorbate, carotenoids, lipoic acid, phenolic compounds (e.g., flavonoids, flavones, flavonols), potassium ascorbate, sodium ascorbate, thiols (e.g., glutathione, lipoic acid, N-acetyl cyst
- Non-limiting examples of antioxidants include those that are soluble in the cell membrane (e.g., alpha tocopherol (vitamin E), ascorbyl palmitate) and those that are soluble in water (e.g., ascorbic acid and isomers or ascorbic acid, sodium or potassium salts of ascorbic acid or isomers or ascorbic acid, glutathione, sodium or potassium salts of glutathione).
- vitamin E vitamin E
- ascorbyl palmitate those that are soluble in water
- water e.g., ascorbic acid and isomers or ascorbic acid, sodium or potassium salts of ascorbic acid or isomers or ascorbic acid, glutathione, sodium or potassium salts of glutathione.
- use of a membrane-soluble antioxidant necessitates the addition of one or more surfactants to adequately disperse the antioxidant within the inoculant composition.
- the inoculant composition is/comprises ascorbic acid and/or glutathione.
- the inoculant composition comprises one or more hygroscopic polymers (e.g., hygroscopic agars, albumins, alginates, carrageenans, celluloses, gums (e.g., cellulose gum, guar gum, gum arabic, gum combretum, xantham gum), methyl celluloses, nylons, pectins, polyacrylic acids, polycaprolactones, polycarbonates, polyethylene glycols (PEG), polyethylenimines (PEI), polylactides, polymethylacrylates (PMA), polyurethanes, polyvinyl alcohols (PVA), polyvinylpyrrolidones (PVP), propylene glycols, sodium carboxymethyl celluloses and/or starches).
- hygroscopic polymers e.g., hygroscopic agars, albumins, alginates, carrageenans, celluloses, gums (e.g., cellulose
- Non-limiting examples of polymers include AGRIMERTM polymers (e.g., 30, AL-10 LC, AL-22, AT/ATF, VA 3E, VA 31, VA 5E, VA 51, VA 6, VA 6E, VA 7E, VA 71, VEMA AN-216, VEMA AN-990, VEMA AN-1200, VEMA AN-1980, VEMA H-815MS; Ashland Specialty Ingredients, Wilmington, Del.), EASYSPERSETM polymers (Ashland Specialty Ingredients, Wilmington, Del.); DISCOTM AG polymers (e.g., L-250, L-280, L-285, L-286, L-320, L-323, L-517, L-519, L-520, L800; Incotec Inc., Salinas, Calif.), KELZAN® polymers (Bri-Chem Supply Ltd., Calgary, Alberta, CA), TICAXAN® xanthan powders, such as PRE-HYDRATED® TICAXAN® Rapid-3 Powder (TIC
- the inoculant composition comprises one or more UV protectants (e.g., one or more aromatic amino acids (e.g., tryptophan, tyrosine), carotenoids, cinnamates, lignosulfonates (e.g., calcium lignosulfonate, sodium lignosulfonate), melanins, mycosporines, polyphenols and/or salicylates).
- UV protectants e.g., one or more aromatic amino acids (e.g., tryptophan, tyrosine), carotenoids, cinnamates, lignosulfonates (e.g., calcium lignosulfonate, sodium lignosulfonate), melanins, mycosporines, polyphenols and/or salicylates).
- UV protectants include Borregaard LignoTechTM lignosulfonates (e.g., Borresperse 3A, Borresperse CA, Borresperse NA, Marasperse AG, Norlig A, Norlig 11D, Ufoxane 3A, Ultrazine NA, Vanisperse CB; Borregaard Lignotech, Sarpsborg, Norway) and combinations thereof.
- Borregaard LignoTechTM lignosulfonates e.g., Borresperse 3A, Borresperse CA, Borresperse NA, Marasperse AG, Norlig A, Norlig 11D, Ufoxane 3A, Ultrazine NA, Vanisperse CB; Borregaard Lignotech, Sarpsborg, Norway
- compositions of the present disclosure may comprise any suitable excipient(s), including, but not limited to, dispersants, drying agents, anti-freezing agents, flowability agents, safeners, anti-settling agents, pH buffers and adhesives.
- Inoculant compositions of the present disclosure may comprise any suitable dispersant(s), including, but not limited to, surfactants and wetting agents. Selection of appropriate dispersants will depend on the intended application(s) and the microorganism(s) present in the inoculant composition. In general, the dispersant(s) will have low toxicity for the microorganism(s) in the inoculant composition and for the medium(s) to which the inoculant composition is to be applied.
- suitable dispersant(s) including, but not limited to, surfactants and wetting agents. Selection of appropriate dispersants will depend on the intended application(s) and the microorganism(s) present in the inoculant composition. In general, the dispersant(s) will have low toxicity for the microorganism(s) in the inoculant composition and for the medium(s) to which the inoculant composition is to be applied.
- Non-limiting examples of dispersants include AtloxTM (e.g., 4916, 4991; Croda International PLC, Edison, N.J.), Atlox METASPERSETM (Croda International PLC, Edison, N.J.), BIO-SOFT® (e.g., N series, such as N1-3, N1-7, N1-5, N1-9, N23-3, N2.3-6.5, N25-3, N25-7, N25-9, N91-2.5, N91-6, N91-8; Stepan Company, Northfield, Ill.), MAKON® nonionic surfactants (e.g., DA-4, DA-6 and DA-9; Stepan Company, Northfield, Ill.), MORWET® powders (Akzo Nobel Surface Chemistry LLC, Chicago, Ill.), MULTIWETTM surfactants (e.g., MO-85P-PW-(AP); Croda International PLC, Edison, N.J.), SILWET® L-77 (Helena Chemical Company
- inoculant compositions of the present disclosure comprise one or more anionic surfactants.
- the inoculant composition comprises one or more water-soluble anionic surfactants and/or one or more water-insoluble anionic surfactants, optionally one or more anionic surfactants selected from the group consisting of alkyl carboxylates (e.g., sodium stearate), alkyl sulfates (e.g., alkyl lauryl sulfate, sodium lauryl sulfate), alkyl ether sulfates, alkyl amido ether sulfates, alkyl aryl polyether sulfates, alkyl aryl sulfates, alkyl aryl sulfonates, alkyl sulfonates, alkyl amide sulfonates, alkyl aryl sulfonates, alkyl benzene sulfonates, alkyl dipheny
- inoculant compositions of the present disclosure comprise one or more cationic surfactants.
- the inoculant composition comprises one or more pH-dependent amines and/or one or more quaternary ammonium cations, optionally one or more cationic surfactants selected from the group consisting of alkyltrimethylammonium salts (e.g., cetyl trimethylammonium bromide, cetyl trimethylammonium chloride), cetylpyridinium chloride, benzalkonium chloride, benzethonium chloride, 5-Bromo-5-nitro-1,3-dioxane, dimethyldioctadecylammonium chloride, cetrimonium bromide, dioctadecyldimethylammonium bromide and/or octenidine dihydrochloride.
- alkyltrimethylammonium salts e.g., cetyl trimethylammonium bromide, cetyl trimethylammonium
- inoculant compositions of the present disclosure comprise one or more nonionic surfactants.
- the inoculant composition comprises one or more water-soluble nonionic surfactants and/or one or more water-insoluble nonionic surfactants, optionally one or more nonionic surfactants selected from the group consisting of alcohol ethoxylates (e.g., TERGITOLTM 15-S surfactants, such as TERGITOLTM15-S-9 (The Dow Chemical Company, Midland, Mich.)), alkanolamides, alkanolamine condensates, carboxylic acid esters, cetostearyl alcohol, cetyl alcohol, cocamide DEA, dodecyldimethylamine oxides, ethanolamides, ethoxylates of glycerol ester and glycol esters, ethylene oxide polymers, ethylene oxide-propylene oxide copolymers, glucoside alkyl ethers, glycerol alkyl ether
- inoculant compositions of the present disclosure comprise at least one nonionic surfactant.
- the inoculant composition comprises at least one water insoluble nonionic surfactant and at least one water soluble nonionic surfactant.
- inoculant compositions of the present disclosure comprise a combination of nonionic surfactants having hydrocarbon chains of substantially the same length.
- inoculant compositions of the present disclosure comprise one or more zwitterionic surfactants.
- the inoculant composition comprises one or more betaines and/or one or more sultaines, optionally one or more zwitterionic surfactants selected from the group consisting of 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate, cocamidopropyl betaine, cocamidopropyl hydroxysultaine, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine and/or one or more sphingomyelins.
- inoculant compositions of the present disclosure comprise one or more soaps and/or organosilicone surfactants. According to some embodiments, the inoculant composition comprises one or more alkali metal salts of fatty acids.
- inoculant compositions of the present disclosure comprise one or more wetting agents.
- the inoculant composition comprises one or more naphthalene sulfonates, optionally one or more alkyl naphthalene sulfonates (e.g., sodium alkyl naphthalene sulfonate), one or more isopropyl naphthalene sulfonates (e.g., sodium isopropyl naphthalene sulfonate) and/or one or more butyl naphthalene sulfonates (e.g., sodium n-butyl naphthalene sulfonate).
- alkyl naphthalene sulfonates e.g., sodium alkyl naphthalene sulfonate
- isopropyl naphthalene sulfonates e.g., sodium isopropyl naphthalene
- Inoculant compositions of the present disclosure may comprise any suitable drying agent(s), including, but not limited to, drying powders.
- drying agents include AEROSIL® hydrophobic fumed silica powders (Evonik Corporation, Parsippany, N.J.), BENTOLITE® powders (BYK-Chemie GmbH, Wesel, Germany), INCOTEC® powders (INCOTEC Inc., Salinas, Calif.), SIPERNAT® silica powders (Evonik Corporation, Parsippany, N.J.) and combinations thereof.
- inoculant compositions of the present disclosure comprise calcium stearate, clay (e.g., attapulgite clay, montmorillonite clay), graphite, magnesium stearate, magnesium sulfate, powdered milk, silica (e.g., fumed silica, hydrophobically-coated silica, precipitated silica), soy lecithin and/or talc.
- clay e.g., attapulgite clay, montmorillonite clay
- graphite e.g., calcium stearate
- magnesium stearate e.g., magnesium stearate
- magnesium sulfate e.g., magnesium stearate
- powdered milk e.g., silica (e.g., fumed silica, hydrophobically-coated silica, precipitated silica), soy lecithin and/or talc.
- silica e.g., fumed silica,
- Inoculant compositions of the present disclosure may comprise any suitable anti-freezing agent(s), including, but not limited to, ethylene glycol, glycerin, propylene glycol and urea.
- suitable anti-freezing agent(s) including, but not limited to, ethylene glycol, glycerin, propylene glycol and urea.
- Inoculant compositions of the present disclosure may comprise any flowability agent to improve the lubricity of treated materials.
- the flowability agent may comprise one or more liquid lubricants, solid lubricants, liquid emulsions, or suspensions of solid lubricants.
- Non-limiting examples of flowability agents include, for example, lubricants such as fats and oils, natural and synthetic waxes, graphite, talc, fluoropolymers (e.g., polytetrafluoroethylene), and solid lubricants such as molybdenum disulfide and tungsten disulfide.
- the flowability agent comprises a wax material.
- Non-limiting examples of wax materials that can be incorporated into the liquid treatment composition include plant and animal-derived waxes such as carnauba wax, candelilla wax, ouricury wax, beeswax, spermaceti, and petroleum derived waxes, such as paraffin wax.
- the flowability agent comprises carnauba wax.
- the flowability agent comprises an oil.
- the flowability agent may comprise soybean oil.
- suitable wax materials suitable for use as flowability agents include AQUAKLEAN 418 supplied by Micro Powders, Inc. (an anionic aqueous emulsion comprising extra light carnauba wax at 35% solids content).
- compositions of the present disclosure may comprise any suitable safener(s), including, but not limited to, napthalic anhydride.
- Inoculant compositions of the present disclosure may comprise any suitable pH buffer(s), including, but not limited to, potassium phosphate monobasic and potassium phosphate dibasic.
- the inoculant composition comprises one or more pH buffers selected to provide a composition having a pH of less than 10, typically from about 4.5 to about 9.5, from about 6 to about 8, or about 7.
- Inoculant compositions of the present disclosure may comprise any suitable anti-settling agent(s), including, but not limited to, polyvinyl acetate, polyvinyl alcohols with different degrees of hydrolysis, polyvinylpyrrolidones, polyacrylates, acrylate-, polyol- or polyester-based paint system binders which are soluble or dispersible in water, moreover copolymers of two or more monomers such as acrylic acid, methacrylic acid, itaconic acid, maleic acid, fumaric acid, maleic anhydride, vinylpyrrolidone, ethylenically unsaturated monomers such as ethylene, butadiene, isoprene, chloroprene, styrene, divinylbenzene, ot-methylstyrene or p-methylstyrene, further vinyl halides such as vinyl chloride and vinylidene chloride, additionally vinyl esters such as vinyl acetate, vinyl propionate or vinyl stearate, more
- Inoculant compositions of the present disclosure may comprise any suitable adhesive(s), including, but not limited to, adhesive compositions comprising, consisting essentially of or consisting of one or more disaccharides (e.g. maltose), gums (e.g., cellulose gum, guar gum, gum arabic, gum combretum, xantham gum), maltodextrins (e.g., one or more maltodextrins (each and/or collectively) having a DEV of about 10 to about 20), monosaccharides, oils (e.g., mineral oil, olive oil, peanut oil, soybean oil and/or sunflower oil) and/or oligosaccharides.
- adhesive compositions comprising, consisting essentially of or consisting of one or more disaccharides (e.g. maltose), gums (e.g., cellulose gum, guar gum, gum arabic, gum combretum, xantham gum), maltodextrins (e.
- Inoculant compositions of the present disclosure may comprise any suitable effect pigment(s).
- Effect pigments which are sometimes also referred to in the art as “pearl pigments,” are a class of materials that provide reflectivity, shine, and/or a pearlescent effect when applied as a coating.
- the effect pigment is in the form of a powder comprising a substrate material and a metal oxide coating.
- the effect pigment may comprise a substrate material including but not limited to talc, silicate materials (e.g., mica), clay minerals, calcium carbonate, kaolin, phlogopite, alumina, and similar substances.
- the substrate material comprises a hydrophilic material.
- the substrate material may be coated with a semi-transparent layer of a metal oxide, including but not limited to titanium dioxide, iron oxide, chromium oxide, or zirconium oxide.
- the effect pigment comprises metal powder or metal flakes.
- the metal powder or metal flakes may comprise a metal including, but not limited to aluminum, copper, silver, or bronze.
- the effect pigment comprises a silicate based substrate.
- particulate silicates that can be incorporated into the dry powder coating include mica coated with titanium dioxide (e.g., SUNMICA FINE WHITE 2800102, which is commercially available from Sun Chemical Corp.).
- the dry powder has a mean particle size of from about 1 to about 25 microns.
- Inoculant compositions of the present disclosure may comprise any growth medium suitable for culturing one or more of the microorganisms in the inoculant composition.
- inoculant compositions of the present disclosure comprise Czapek-Dox medium, glycerol yeast extract, mannitol yeast extract, potato dextrose broth and/or YEM media.
- Carriers, stabilizing compounds, dispersants, drying agents, safeners, flowability agents, anti-settling agents, buffers, adhesives, etc. may be incorporated into inoculant compositions of the present disclosure in any suitable amount(s)/concentration(s).
- the absolute value of the amount/concentration that is/are sufficient to cause the desired effect(s) may be affected by factors such as the type, size and volume of material to which the compositon will be applied, the type(s) of microorganisms in the composition, the number of microorganisms in the composition, the stability of the microorganisms in the composition and storage conditions (e.g., temperature, relative humidity, duration).
- inoculant compositions of the present disclosure comprise one or more carriers in an amount/concentration of about 1 to about 99% or more (by weight, based upon the total weight of the inoculant composition).
- inoculant compositions of the present disclosure may comprsise about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98 or 99% (by weight) of one or more non-aqueous carriers.
- inoculant compositions of the present disclosure comprise one or more stabilizing compounds in an amount/concentration of about 0.0001 to about 95% or more (by weight, based upon the total of the inoculant composition).
- inoculant compositions of the present disclosure may comprise about 0.0001 to about 0.001, about 0.001 to about 1%, about 0.25 to about 5%, about 1 to about 10%, about 5 to about 25%, about 10% to about 30%, about 20% to about 40%, about 25% to about 50%, about 30 to about 60%, about 50 to about 75%, or about 75 to about 95% (by weight), optionally about 0.0005, 0.001, 0.002, 0.003, 0.004, 0.005, 0.0075, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20,
- inoculant compositions of the present disclosure comprise one or more stabilizing compounds at a concentration of about 1 ⁇ 10 ⁇ 20 M to about 1 ⁇ 10 ⁇ 1 M.
- inoculant compositions of the present disclosure may comprise about 1 ⁇ 10 ⁇ 15 M to about 1 ⁇ 10 ⁇ 10 M, about 1 ⁇ 10 ⁇ 14 M to about 1 ⁇ 10 ⁇ 8 M, about 1 ⁇ 10 ⁇ 14 M to about 1 ⁇ 10 ⁇ 6 M, about 1 ⁇ 10 ⁇ 12 M to about 1 ⁇ 10 ⁇ 8 M, about 1 ⁇ 10 ⁇ 12 M to about 1 ⁇ 10 ⁇ 6 M, about 1 ⁇ 10 ⁇ 10 M to about 1 ⁇ 10 ⁇ 6 M, or about 1 ⁇ 10 ⁇ 8 M to about 1 ⁇ 10 ⁇ 2 M, optionally about 1 ⁇ 10 ⁇ 20 M, 1 ⁇ 10 ⁇ 19 M, 1 ⁇ 10 ⁇ 18 M, 1 ⁇ 10 ⁇ 17 M, 1 ⁇ 10 ⁇ 16 M, 1 ⁇ 10 ⁇ 15 M, 1 ⁇ 10 ⁇ 14 M, 1 ⁇ 10 ⁇ 13 M, 1
- inoculant compositions of the present disclosure comprise one or more monosaccharides in an amount/concentration of about 0.005 to about 50% (by weight) of the inoculant composition.
- inoculant compositions of the present disclosure may comprise about/at least/less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.75, 1, 1.25, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 10, 15, 20, 25% (by weight) of one or more monosaccharides (e.g., arabinose, fructose and/or glucose).
- monosaccharides e.g., arabinose, fructose and/or glucose
- one or more monosaccharides is/are present in a concentration ranging from about 1 ⁇ 10 ⁇ 20 M to about 1 ⁇ 10 ⁇ 1 M.
- one or more monosaccharides may be included at a concentration of about/at least/less than 1 ⁇ 10 ⁇ 20 M, 1 ⁇ 10 ⁇ 19 M, 1 ⁇ 10 ⁇ 18 M, 1 ⁇ 10 ⁇ 17 M, 1 ⁇ 10 ⁇ 16 M, 1 ⁇ 10 ⁇ 15 M, 1 ⁇ 10-14 M, 1 ⁇ 10 ⁇ 13 M, 1 ⁇ 10 ⁇ 12 M, 1 ⁇ 10 ⁇ 11 M, 1 ⁇ 10 ⁇ 1 ° M.
- inoculant compositions of the present disclosure comprise one or more disaccharides in an amount/concentration of about 0.005 to about 50% (by weight) of the inoculant composition.
- inoculant compositions of the present disclosure may comprise about/at least/less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.75, 1, 1.25, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 10, 15, 20, 25% (by weight) of one or more disaccharides (e.g., maltose, sucrose and/or trehalose).
- disaccharides e.g., maltose, sucrose and/or trehalose
- one or more disaccharides is/are present in a concentration ranging from about 1 ⁇ 10-20 M to about 1 ⁇ 10 ⁇ 1 M.
- one or more disaccharides may be included at a concentration of about/at least/less than 1 ⁇ 10 ⁇ 20 M, 1 ⁇ 10 ⁇ 19 M, 1 ⁇ 10 ⁇ 18 M, 1 ⁇ 10 ⁇ 17 M, 1 ⁇ 10 ⁇ 16 M, 1 ⁇ 10 ⁇ 15 M, 1 ⁇ 10 ⁇ 14 M, 1 ⁇ 10 ⁇ 13 M, 1 ⁇ 10 ⁇ 12 M, 1 ⁇ 10 ⁇ 11 M, 1 ⁇ 10 ⁇ 10 M.
- inoculant compositions of the present disclosure comprise one or more maltodextrins in an amount/concentration of about 0.001 to about 95% or more (by weight) of the inoculant composition.
- the maltodextrin(s) comprise(s) about 0.001 to about 1%, about 0.25 to about 5%, about 1 to about 10%, about 5 to about 25%, about 10% to about 30%, about 20% to about 40%, about 25% to about 50%, about 50 to about 75%, or about 75 to about 95% (by weight) of the inoculant composition.
- inoculant compositions of the present disclosure may comprise about/at least/less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of one or more maltodextrins (e.g., one or more maltodextrins (each and/or collectively) having a DEV value of about 15 to about 20).
- maltodextrins e.g., one or more maltodextrins (each and/or collectively) having a DEV value of about 15 to about 20.
- inoculant compositions of the present disclosure comprise one or more sugar alcohols in an amount/concentration of about 0.001 to about 95% or more (by weight) of the inoculant composition.
- the sugar alcohol(s) e.g., arabitol, mannitol, sorbitol and/or xylitol
- the sugar alcohol(s) comprise(s) about 0.001 to about 1%, about 0.25 to about 5%, about 1 to about 10%, about 5 to about 25%, about 10% to about 30%, about 20% to about 40%, about 25% to about 50%, about 50 to about 75%, or about 75 to about 95% (by weight) of the inoculant composition.
- inoculant compositions of the present disclosure may comprise about/at least/less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of one or more sugar alcohols (e.g., arabitol, mannitol, sorbitol and/or xylitol).
- sugar alcohols e.g., arabitol, mannitol, sorbitol and/or xylitol
- inoculant compositions of the present disclosure comprise one or more humic acids in an amount/concentration of about 0.001 to about 95% or more (by weight) of the inoculant composition.
- the humic acid(s) e.g., potassium humate
- the humic acid(s) comprise(s) about 0.001 to about 1%, about 0.25 to about 5%, about 1 to about 10%, about 5 to about 25%, about 10% to about 30%, about 20% to about 40%, about 25% to about 50%, about 50 to about 75%, or about 75 to about 95% (by weight) of the inoculant composition.
- inoculant compositions of the present disclosure may comprise about/at least/less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of one or more humic acids (e.g., potassium humate and/or sodium humate).
- humic acids e.g., potassium humate and/or sodium humate
- inoculant compositions of the present disclosure comprise one or more UV protectants in an amount/concentration of about 0.0001 to about 5% or more (by weight) of the inoculant composition.
- the UV protectant(s) e.g., calcium lignosulfate and/or sodium lignosulfate
- the UV protectant(s) comprise(s) about 0.0001 to about 0.001, about 0.001 to about 1%, about 0.25 to about 5%, (by weight) of the inoculant composition.
- inoculant compositions of the present disclosure may comprise about/at least/less than 0.0005, 0.001, 0.002, 0.003, 0.004, 0.005, 0.0075, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.75, 1, 1.25, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5% or more (by weight) of one or more UV protectants (e.g., calcium lignosulfate and/or sodium lignosulfate).
- UV protectants e.g., calcium lignosulfate and/or sodium lignosulfate
- inoculant compositions of the present disclosure comprise one or more oxidation control components in an amount/concentration of about 0.0001 to about 5% or more (by weight) of the composition.
- inoculant compositions of the present disclosure may comprise about/at least/less than 0.0005, 0.001, 0.002, 0.003, 0.004, 0.005, 0.0075, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.75, 1, 1.25, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5% of one or more oxidation control components.
- the amount/concentration of oxidation control components is about 0.005 to about 2% (by weight) of the composition.
- the oxidation control component(s) is/are present in a concentration ranging from about 1 ⁇ 10 ⁇ 20 M to about 1 ⁇ 10 ⁇ 1 M.
- one or more oxidation control components may be added at a concentration of about/at least/less than 1 ⁇ 10 ⁇ 20 M, 1 ⁇ 10 ⁇ 19 M, 1 ⁇ 10 ⁇ 18 M, 1 ⁇ 10 ⁇ 17 M, 1 ⁇ 10 ⁇ 16 M, 1 ⁇ 10 ⁇ 15 M, 1 ⁇ 10 ⁇ 14 M, 1 ⁇ 10 ⁇ 13 M, 1 ⁇ 10 ⁇ 12 M, 1 ⁇ 10 11 M, 1 ⁇ 10 10 M.
- inoculant compositions of the present disclosure comprise one or more commercial antioxidants used in accordance with the manufacturer's recommended amounts/concentrations. In some embodiments, inoculant compositions of the present disclosure comprise one or more commercial oxygen scavengers used in accordance with the manufacturer's recommended amounts/concentrations.
- inoculant compositions of the present disclosure comprise one or more stabilizing compounds in an amount/concentration sufficient to ensure the fungal cells and/or spores remain viable following:
- cryopreservation at or below ⁇ 80° C. for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- inoculant compositions of the present disclosure comprise one or more stabilizing compounds in an amount/concentration sufficient to ensure at least 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% of the fungal cells and/or spores remain viable following:
- cryopreservation at or below ⁇ 80° C. for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- inoculant compositions of the present disclosure comprise one or more stabilizing compounds in an amount/concentration sufficient to ensure at least 1 ⁇ 10 1 , 1 ⁇ 10 2 , 1 ⁇ 10 3 , 1 ⁇ 10 4 , 1 ⁇ 10 5 , 1 ⁇ 10 6 , 1 ⁇ 10 7 , 1 ⁇ 10 8 , 1 ⁇ 10 9 , 1 ⁇ 10 10 or more colony-forming units of the fungal cells and/or spores remain viable per gram and/or milliliter of inoculant composition following:
- inoculant compositions of the present disclosure comprise one or more stabilizing compounds in an amount/concentration sufficient to ensure the deliquescence relative humidity (DRH) of the inoculant composition is less than 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85 or 90 at the temperature(s) at which the composition is to be stored (e.g., 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C.).
- DRRH deliquescence relative humidity
- inoculant compositions of the present disclosure comprise two or more stabilizing compounds that synergistically enhance the stability and/or survival of the fungal cells and/or spores.
- Stabilizing compounds may be incorporated into inoculant compositions of the present disclosure in any suitable ratio(s).
- inoculant compositions of the present disclosure comprise one or more maltodextrins and one or more monosaccharides, disaccharides, sugar alcohols and/or humic acids in a maltodextrin:(monosaccharide, disaccharide, sugar alcohol and/or humic acid) ratio of about 5:95, 10:90, 15:85, 20:80, 25:75, 30:70, 35:65, 40:60, 45:55, 50:50, 55:45, 60:40, 65:35, 70:30, 75:25, 80:20, 85:15, 90:10, 95:5.
- inoculant compositions of the present disclosure may comprise one or more maltodextrins (e.g., one or more maltodextrins (each and/or collectively) having a DEV of about 15 to about 20) and one or more sugar alcohols (e.g., sorbitol and/or xylitol) and/or humic acids (e.g., potassium humate) in a maltodextrin:(sugar alcohol/humic acid) ratio of about 5:95, about 15:85, about 25:75 or about 50:50.
- maltodextrins e.g., one or more maltodextrins (each and/or collectively) having a DEV of about 15 to about 20
- sugar alcohols e.g., sorbitol and/or xylitol
- humic acids e.g., potassium humate
- inoculant compositions of the present disclosure comprise one or more dispersants in an amount/concentration of about 0.001 to about 25% or more (by weight) of the inoculant composition.
- the dispersant(s) comprise(s) 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.004, 0.0045, 0.005, 0.0055, 0.006, 0.0065, 0.007, 0.0075, 0.008, 0.0085, 0.009, 0.0095, 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.02, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9,
- inoculant compositions of the present disclosure may comprise about 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20% or more (by weight) of one or more dispersants (e.g., one or more surfactants and/or wetting agents).
- one or more dispersants e.g., one or more surfactants and/or wetting agents
- inoculant compositions of the present disclosure comprise one or more drying agents in an amount/concentration of about 0.001 to about 95% or more (by weight) of the inoculant composition.
- the drying agent(s) comprise(s) about) 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.004, 0.0045, 0.005, 0.0055, 0.006, 0.0065, 0.007, 0.0075, 0.008, 0.0085, 0.009, 0.0095, 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.02, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9
- inoculant compositions of the present disclosure may comprise about 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of one or more drying agents (e.g., lecithin and/or talc).
- one or more drying agents e.g., lecithin and/or talc
- the inoculant compositions of the present disclosure comprise about 0.5 to about 10 grams of drying powder per liter of inoculant composition.
- inoculant compositions of the present disclosure may comprise about 0.5, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10 grams or more of drying powder per liter of inoculant composition.
- inoculant compositions of the present disclosure comprise one or more buffers in an amount/concentration of about 0.0001 to about 5% or more (by weight) of the inoculant composition.
- the buffer(s) comprise(s) about 0.0001, 0.0002, 0.0003, 0.0004, 0.0005, 0.0006, 0.0007, 0.0008, 0.0009, 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.004, 0.0045, 0.005, 0.0055, 0.006, 0.0065, 0.007, 0.0075, 0.008, 0.0085, 0.009, 0.0095, 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.02, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1 to about 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8
- inoculant compositions of the present disclosure may comprise about 0.0005, 0.00075, 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5% or more (by weight) of one or more (by
- inoculant compositions of the present disclosure comprise one or more commercial carriers, antioxidants, oxygen scavengers, hygroscopic polymers, UV protectants, disperants, drying agents, anti-freezing agents, buffers and/or adhesives used in accordance with the manufacturer's recommended amounts/concentrations.
- the fungal strain(s) of the present disclosure is/are the only microbial strain(s) in inoculant compositions of the present disclosure.
- inoculant compositions of the present disclosure comprise one or more microorganisms in addition to the fungal strain(s) of the present disclosure. Any suitable microorganism(s) may be added. Selection of additional microbes (if any) will depend on the intended application(s).
- Non-limiting examples of bacteria that may be included in inoculant compositions of the present disclosure include Azospirillum brasilense INTA Az-39, Bacillus amyloliquefaciens D747, Bacillus amyloliquefaciens NRRL B 50349, Bacillus amyloliquefaciens TJ1000, Bacillus amyloliquefaciens FZB24, Bacillus amyloliquefaciens FZB42, Bacillus amyloliquefaciens IN937a, Bacillus amyloliquefaciens IT-45, Bacillus amyloliquefaciens TJ1000, Bacillus amyloliquefaciens MBI600, Bacillus amyloliquefaciens BS27 (deposited as NRRL B-5015), Bacillus amyloliquefaciens BS2084 (deposited as NRRL B-50013), Bacillus amyloliquefaciens 15
- Non-limiting examples of fungi that may be included in inoculant compositions of the present disclosure include Gliocladium virens ATCC 52045, Gliocladium virens GL-21, Glomus intraradices RTI-801 , Metarhizium anisopliae F52, PENI, Trichoderma asperellum SKT-1, Trichoderma asperellum ICC 012, Trichoderma atroviride LC52, Trichoderma atroviride CNCM 1-1237 , Trichoderma fertile JM41R, Trichoderma gamsii ICC 080, Trichoderma hamatum ATCC 52198, Trichoderma harzianum ATCC 52445, Trichoderma harzianum KRL-AG2, Trichoderma harzianum T-22, Trichoderma harzianum TH-35, Trichoderma harzianum T-39, Trichoderma harzianum ICC012, Trichoderma reesi ATCC 28217
- mycorrhizal fungi that may be included in inoculant compositions of the present disclosure include mycorrhizal strains such as Gigaspora margarita, Glomus aggregatum, Glomus brasilianum, Glomus clarum, Glomus deserticola, Glomus etunicatum, Glomus intraradices, Glomus monosporum, Glomus mosseae, Laccaria bicolor, Laccaria laccata, Paraglomus brazilianum, Pisolithus tinctorius, Rhizopogon amylopogon, Rhizopogon fulvigleba, Rhizopogon luteolus, Rhizopogon villosuli, Scleroderma cepa and Scleroderma citrinum and combinations thereof.
- mycorrhizal strains such as Gigaspora margarita, Glomus aggregatum, Glomus brasilianum, Glomus clarum, Glomus deserticola, Glomus etunicatum, Glomus intraradices
- microorganisms that may be added to inoculant compositions of the present disclosure can be found in Appendix A.
- Additional microorganisms may be incorporated into inoculant compositions of the present disclosure in any suitable amount(s)/concentration(s).
- the absolute value of the amount/concentration that is/are sufficient to cause the desired effect(s) may be affected by factors such as the type, size and volume of material to which the composition will be applied, the microorganisms in the composition, the number of microorganisms in the composition, the stability of the microorganisms in the composition and storage conditions (e.g., temperature, relative humidity, duration).
- factors e.g., temperature, relative humidity, duration.
- one or more additional microorganisms is/are present in an amount ranging from about 1 ⁇ 10 1 to about 1 ⁇ 10 12 colony-forming units (cfu) per gram and/or milliliter of inoculant composition.
- the inoculant composition comprises about 1 ⁇ 10 1 , 1 ⁇ 10 2 , 1 ⁇ 10 3 , 1 ⁇ 10 4 , 1 ⁇ 10 5 , 1 ⁇ 10 6 , 1 ⁇ 10 7 , 1 ⁇ 10 8 , 1 ⁇ 10 9 , 1 ⁇ 10 10 , 1 ⁇ 10 11 , 1 ⁇ 10 12 or more cfu of one or more additional microorganisms per gram and/or milliliter of inoculant composition (e.g., about 1 ⁇ 10 4 to about 1 ⁇ 10 9 cfu/g of Bacillus amyloliquefaciens TJ1000 (also known as 1BE, isolate ATCC BAA-390), BRADY, Metarhizium anisopliae F52, PENI, Trichoderma .
- inoculant compositions of the present disclosure comprise at least 1 ⁇ 10 4 , 1 ⁇ 10 5 , 1 ⁇ 10 6 , 1 ⁇ 10 7 , 1 ⁇ 10 8 , 1 ⁇ 10 9 , 1 ⁇ 10 10 , 1 ⁇ 10 11 , 1 ⁇ 10 12 cfu of one or more additional microorganisms per gram and/or millilitre of inoculant composition.
- spores from one or more additional microorganims comprise about 0.1 to about 90% (by weight) of the inoculant composition.
- the inoculant composition comprises about 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of microbial spores from one or more additional microorganisms (e.g., about 10% Bacillus amyloliquefaciens TJ1000 , Metarhizium anisopliae F52, Penicillium bilaiae ATCC 20851, Penicillium bilaia
- additional microorganisms
- the amount/concentration of microbial spores from one or more additional microorganisms is about 1 to about 25%, about 5 to about 20%, about 5 to about 15%, about 5 to about 10% or about 8 to about 12% (by weight) of the inoculant composition.
- additional microorganisms in inoculant compositions of the present disclosure may comprise vegetative cells and/or dormant spores. According to some embodiments, at least 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98, 99% or more additional microorganims are present in inoculant compostions of the present disclosure as vegetative cells.
- At least 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98, 99% or more additional microorganims are present in inoculant compostions of the present disclosure as spores.
- compositions of the present disclosure may be formulated as any suitable type of composition.
- inoculant compositions of the present disclosure are formulated as amorphous solids.
- inoculant compositions of the present disclosure are formulated as amorphous liquids.
- inoculant compositions of the present disclosure are formulated as wettable powders.
- inoculant compositions of the present disclosure are formulated as liquid compositions that are subsequently dried to produce a powder or granuale.
- liquid inoculant compositions of the present disclosure are drum dried, evaporation dried, fluidized bed dried, freeze dried, spray dried, spray-freeze dried, tray dried and/or vacuum dried to produce powders/granuales.
- Such powders/granuales may be further processed using any suitable method(s), including, but not limited to, flocculation, granulation and milling, to achieve a desired particle size or physical format.
- the precise method(s) and parameters of processing dried powders/granuales that are appropriate in a given situation may be affected by factors such as the desired particle size(s), the type, size and volume of material to which the compositon will be applied, the type(s) of microorganisms in the composition, the number of microorganisms in the composition, the stability of the microorganisms in the composition and the storage conditions (e.g., temperature, relative humidity, duration). Those skilled in the art will understand how to select appropriate methods and parameters using routine experiments.
- inoculant compositions of the present disclosure are frozen for cryopreservation.
- liquid inoculant compositions of the present disclosure are flash-frozen and stored in a cryopreservation storage unit/facility.
- the precise method(s) and parameters of freezing and preserving inoculant compositions of the present disclosure that are appropriate in a given situation may be affected by factors such as the type(s) of microorganisms in the composition, the number of microorganisms in the composition, the stability of the microorganisms in the composition and the storage conditions (e.g., temperature, relative humidity, duration). Those skilled in the art will understand how to select appropriate methods and parameters using routine experiments.
- Inoculant compositions of the present disclosure may be formulated as aqueous or non-aqueous compositions. In some embodiments, inoculant compositions of the present disclosure comprise no water. In some embodiments, inoculant compositions of the present disclosure comprise a trace amount of water.
- inoculant compositions of the present disclosure comprise less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75 or 5% water by weight, based upon the total weight of the composition.
- inoculant compositions of the present disclosure are formulated to have a pH of about 4.5 to about 9.5. In some embodiments, inoculant compositions of the present disclosure have a pH of about 6 to about 7.5. In some embodiments, inoculant compositions of the present disclosure have a pH of about 5, 5.5, 6, 6.5, 7, 7.5, 8 or 8.5.
- inoculant compositions of the present disclosure may contain a variety of carriers, stabilizers, dispersants, etc. It is to be understood that the components to be included in the inoculant composition and the order in which components are incorporated into the inoculant composition may be chosen or designed to maintain or enhance the dispersion, stability and/or survival of fungal cells/spores of the present disclosure during storage, distribution, and/or application of the inoculant composition.
- inoculant compositions of the present disclosure are non-naturally occurring compositions.
- the inoculant composition comprises one or more non-naturally occurring components.
- the inoculant composition comprises a non-naturally occurring combination of naturally occurring components.
- kits comprising, consisting essentially of, or consisting of two or more containers, each comprising one or more components of an inoculant composition of the present disclosure.
- kits comprising, consisting essentially of, or consisting of two or more containers, each comprising one or more components of an inoculant composition of the present disclosure.
- kits further comprises one or more oxygen scavengers, such as activated carbon, ascorbic acid, iron powder, mixtures of ferrous carbonate and metal halide catalysts, sodium chloride and/or sodium hydrogen carbonate.
- oxygen scavengers such as activated carbon, ascorbic acid, iron powder, mixtures of ferrous carbonate and metal halide catalysts, sodium chloride and/or sodium hydrogen carbonate.
- the containers may comprise any suitable material(s), including, but not limited to, materials that reduce the amount of light, moisture and/or oxygen that contact the inoculant composition when the container is sealed.
- the containers comprise, consist essentially of, or consist of a material having light permeability of less than about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70 or 75%.
- the containers comprise, consist essentially of, or consist of a material having an oxygen transmission rate of less than about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, or 500 cm 3 /m 2 ⁇ day (as measured in accordance with ASTM D3985).
- the containers reduce the amount of ambient light that reaches said inoculant composition by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% when sealed.
- the containers reduce the amount of ambient moisture that reaches said inoculant composition by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% when sealed.
- the containers reduce the amount of ambient oxygen that reaches said inoculant composition by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% when sealed.
- the present disclosure extends to methods and uses for fungal strains and inoculant compositions of the present disclosure.
- methods and uses of the present disclosure comprise, consist essentially of or consist of applying one or more fungal strains (or inoculant compositions) of the present disclosure to a medium comprising one or more insoluble forms of phosphorous (e.g., insoluble phosphates) in an amount/concentration effective to increase the amount/concentration of soluble phosphate in said medium.
- a fungal strains or inoculant compositions of the present disclosure
- a medium comprising one or more insoluble forms of phosphorous (e.g., insoluble phosphates) in an amount/concentration effective to increase the amount/concentration of soluble phosphate in said medium.
- the fungal strain(s)/inoculant composition(s) is/are applied in an amount/concentration effective to increase the amount/concentration of soluble phosphate by at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200% or more as compared to a control medium (e.g., an identical medium treated with a control composition that is identical to the inoculant composition of the present disclosure except that it lacks one or more of the fungal strains of the present disclosure found in the inoculant composition of the present disclosure).
- a control medium e.g., an identical medium treated with a control composition that is identical to the inoculant composition of the present disclosure except that it lacks one or more of the fungal strains of the present disclosure found in the inoculant composition of the present disclosure.
- methods and uses of the present disclosure comprise, consist essentially of or consist of introducing one or more fungal strains (or inoculant compositions) of the present disclosure into a medium comprising one or more insoluble forms of phosphorous (e.g., insoluble phosphates) in an amount/concentration effective to increase the amount/concentration of soluble phosphate in said medium.
- a medium comprising one or more insoluble forms of phosphorous (e.g., insoluble phosphates) in an amount/concentration effective to increase the amount/concentration of soluble phosphate in said medium.
- the fungal strain(s)/inoculant composition(s) is/are introduced in an amount/concentration effective to increase the amount/concentration of soluble phosphate by at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200% or more as compared to a control medium (e.g., an identical medium treated with a control composition that is identical to the inoculant composition of the present disclosure except that it lacks one or more of the fungal strains of the present disclosure found in the inoculant composition of the present disclosure).
- a control medium e.g., an identical medium treated with a control composition that is identical to the inoculant composition of the present disclosure except that it lacks one or more of the fungal strains of the present disclosure found in the inoculant composition of the present disclosure.
- methods and uses of the present disclosure comprise, consist essentially of or consist of applying one or more fungal strains (or inoculant compositions) of the present disclosure to a medium comprising one or more insoluble forms of phosphorous (e.g., insoluble phosphates) in an amount/concentration effective to decrease the amount/concentration of phosphorous that must be added to the plant growth medium in order to achieve a desired outcome.
- a fungal strains or inoculant compositions of the present disclosure
- a medium comprising one or more insoluble forms of phosphorous (e.g., insoluble phosphates) in an amount/concentration effective to decrease the amount/concentration of phosphorous that must be added to the plant growth medium in order to achieve a desired outcome.
- the fungal strain(s)/inoculant composition(s) is/are applied in an amount/concentration effective to decrease the amount/concentration of phosphorous that must be added to the plant growth medium in order to achieve a desired outcome by at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200% or more as compared to a control medium (e.g., an identical medium treated with a control composition that is identical to the inoculant composition of the present disclosure except that it lacks one or more of the fungal strains of the present disclosure found in the inoculant composition of the present disclosure).
- a control medium e.g., an identical medium treated with a control composition that is identical to the inoculant composition of the present disclosure except that it lacks one or more of the fungal strains of the present disclosure found in the inoculant composition of the present disclosure.
- methods and uses of the present disclosure comprise, consist essentially of or consist of introducing one or more fungal strains (or inoculant compositions) of the present disclosure into a medium comprising one or more insoluble forms of phosphorous (e.g., insoluble phosphates) in an amount/concentration effective to decrease the amount/concentration of phosphorous that must be added to the plant growth medium in order to achieve a desired outcome.
- a medium comprising one or more insoluble forms of phosphorous (e.g., insoluble phosphates) in an amount/concentration effective to decrease the amount/concentration of phosphorous that must be added to the plant growth medium in order to achieve a desired outcome.
- the fungal strain(s)/inoculant composition(s) is/are introduced in an amount/concentration effective to decrease the amount/concentration of phosphorous that must be added to the plant growth medium in order to achieve a desired outcome by at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200% or more as compared to a control medium (e.g., an identical medium treated with a control composition that is identical to the inoculant composition of the present disclosure except that it lacks one or more of the fungal strains of the present disclosure found in the inoculant composition of the present disclosure).
- a control medium e.g., an identical medium treated with a control composition that is identical to the inoculant composition of the present disclosure except that it lacks one or more of the fungal strains of the present disclosure found in the inoculant composition of the present disclosure.
- the fungal cells/spores are applied/introduced (in)to the medium at a rate of about 1 ⁇ 10 1 to about 1 ⁇ 10 20 cfu per kilogram of media, optionally about/at least 1 ⁇ 10 4 , 1 ⁇ 10 5 , 1 ⁇ 10 6 , 1 ⁇ 10 7 , 1 ⁇ 10 8 , 1 ⁇ 10 9 , 1 ⁇ 10 10 , 1 ⁇ 10 11 , 1 ⁇ 10 12 , 1 ⁇ 10 13 , 1 ⁇ 10 14 , 1 ⁇ 10 15 fungal cells/spores per kilogram of media.
- an average of about/at least 1 ⁇ 10 3 , 1 ⁇ 10 4 , 1 ⁇ 10 5 , 1 ⁇ 10 6 , 1 ⁇ 10 7 , 1 ⁇ 10 8 , 1 ⁇ 10 9 , 1 ⁇ 10 10 , 1 ⁇ 10 11 , 1 ⁇ 10 12 cfu of P. bilaiae NRRL B-67154 , P. bilaiae NRRL B-67155 , P. bilaiae NRRL B-67156 , P. bilaiae NRRL B-67157 , P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 are applied/introduced (in)to each kilogram of media.
- An inoculant composition comprising, consisting essentially of or consisting of a carrier and one or more fungal strains, said one or more fungal strains comprising, consisting essentially of or consisting of:
- one or more monosaccharides optionally arabinose, fructose and/or glucose;
- disaccharides optionally maltose, sucrose and/or trehalose
- maltodextrins optionally one or more maltodextrins (e.g., one or more maltodextrins (each and/or collectively) having a DEV value of about 15 to about 20;
- sugar alcohols optionally arabitol, mannitol, sorbitol and/or xylitol;
- humic acids optionally potassium humate and/or sodium humate;
- one or more hygroscopic polymers optionally one or more albumins, alginates, celluloses, gums (e.g., cellulose gum, guar gum, gum arabic, gum combretum, xantham gum), methyl celluloses, nylons, pectins, polyacrylic acids, polycarbonates, polyethylene glycols (PEG), polyethylenimines (PEI), polylactides, polymethylacrylates (PMA), polyurethanes, polyvinyl alcohols (PVA), polyvinylpyrrolidones (PVP), propylene glycols, sodium carboxymethyl celluloses and/or starches;
- PEG polyethylene glycols
- PEI polyethylenimines
- PMA polylactides
- PMA polyurethanes
- PVA polyvinyl alcohols
- PVP polyvinylpyrrolidones
- propylene glycols sodium carboxymethyl celluloses and/or starches
- one or more oxidation control components optionally one or more antioxidants (e.g., ascorbic acid, ascorbyl palmitate, ascorbyl stearate, calcium ascorbate, one or more carotenoids, lipoic acid, one or more phenolic compounds (e.g., one or more flavonoids, flavones and/or flavonols), potassium ascorbate, sodium ascorbate, one or more thiols (e.g., glutathione, lipoic acid and/or N-acetyl cysteine), one or more tocopherols, one or more tocotrienols, ubiquinone and/or uric acid) and/or one or more oxygen scavengers, optionally ascorbic acid and/or sodium hydrogen carbonate; and/or
- antioxidants e.g., ascorbic acid, ascorbyl palmitate, ascorbyl stearate, calcium ascorbate, one or more carotenoids, lipoic acid
- UV protectants optionally one or more lignosulfites.
- said one or more stabilizing compounds comprising about 0.0001 to about 10% (by weight) of said composition, optionally about 2 to about 6% (by weight) of said composition, optionally about 0.0005, 0.001, 0.002, 0.003, 0.004, 0.005, 0.0075, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 7, 7.5, 8, 8.5, 9, 9.5 or 10% (by weight) of said composition.
- cryopreservation at or below ⁇ 80° C. for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- said one or more additional microorganisms comprising, consisting essentially of or consisting of Azospirillum brasilense INTA Az-39, Bacillus amyloliquefaciens D747, Bacillus amyloliquefaciens NRRL B-50349, Bacillus amyloliquefaciens TJ1000, Bacillus amyloliquefaciens FZB24, Bacillus amyloliquefaciens FZB42, Bacillus amyloliquefaciens IN937a, Bacillus amyloliquefaciens IT-45, Bacillus amyloliquefaciens TJ1000, Bacillus amyloliquefaciens MBI600, Bacillus amyloliquefaciens BS27 (deposited as NRRL B-5015), Bacillus amyloliquefaciens BS2084 (deposited as NRRL B-50013), Bacillus
- said one or more additional microorganisms comprising, consisting essentially of or consisting of Gliocladium virens ATCC 52045, Gliocladium virens GL-21, Glomus intraradices RTI-801 , Metarhizium anisopliae F52, PENI, Trichoderma asperellum SKT-1, Trichoderma asperellum ICC 012, Trichoderma atroviride LC52, Trichoderma atroviride CNCM 1-1237 , Trichoderma fertile JM41R, Trichoderma gamsii ICC 080, Trichoderma hamatum ATCC 52198, Trichoderma harzianum ATCC 52445, Trichoderma harzianum KRL-AG2, Trichoderma harzianum T-22, Trichoderma harzianum TH-35, Trichoderma harzianum T-39, Trichoderma harzianum ICC012, Trichoderma re
- said one or more additional microorganisms comprising, consisting essentially of or consisting of one or more biopesticides, optionally one or more acaricidal, insecticidal and/or nematicidal microorganisms and one or more fungicidal microorganisms.
- the inoculant composition of any one of claims 22 - 25 said composition comprising about 1 ⁇ 10 3 to about 1 ⁇ 10 12 colony-forming units (cfu) of said one or more additional microorganisms per gram and/or milliliter of inoculant composition, optionally about/at least 1 ⁇ 10 3 , 1 ⁇ 10 4 , 1 ⁇ 10 5 , 1 ⁇ 10 6 , 1 ⁇ 10 7 , 1 ⁇ 10 8 , 1 ⁇ 10 9 , 1 ⁇ 10 10 , 1 ⁇ 10 11 , or 1 ⁇ 10 12 cfu of said one or more additional microorganisms per gram and/or milliliter of inoculant composition.
- composition of any one paragraphs 12-26 wherein said composition is a freeze-, spray- or spray-freeze-dried composition, optionally a freeze-, spray- or spray-freeze-dried powder.
- a kit comprising:
- kit of claim 33 said container reducing the amount of ambient light that reaches said inoculant composition by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% when sealed.
- kit of any one of paragraphs 33-34 said container reducing the amount of ambient oxygen that reaches said inoculant composition by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% when sealed.
- kit of any one of paragraphs 33-35 said container comprising, consisting essentially of, or consisting of a material having light permeability of less than about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70 or 75%.
- kit of any one of paragraphs 33-36 said container comprising, consisting essentially of, or consisting of a material having an oxygen transmission rate of less than about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, or 500 cm 3 /m 2 ⁇ day (as measured in accordance with ASTM D3985).
- kit of any one of paragraphs 33-37 said kit furthering comprising one or more oxygen-absorbing compound, optionally activated carbon, iron powder, sodium chloride, ferrous carbonate, one or more metal halide catalysts and/or sodium hydrogen carbonate.
- P. bilaiae NRRL 67154 Use of P. bilaiae NRRL 67154 , P. bilaiae NRRL 67155 , P. bilaiae NRRL 67156 , P. bilaiae NRRL 67157 , P. bilaiae NRRL 67158, and/or P. bilaiae NRRL 67159 to solubilize phosphate in a medium that comprises one or more insoluble phosphates.
- a method comprising, consisting essentially of, or consisting of:
- a method comprising, consisting essentially of, or consisting of:
- Penicillium bilaiae strains provided by the present disclosure—NRRL 67154, NRRL 67155, NRRL 67156, NRRL 67157, NRRL 67158 and NRRL 67159—was isolated from a soil sample collected in Canada and deposited on Dec. 2, 2015 under the terms of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure at the Agricultural Research Service Culture Collection, 1815 North University Street, Peoria, Ill. 61604, U.S.A.
- Phosphate solubilization assays of 118 isolates from Example 1 were carried out in 96-well microbioreactor plates (EnzyScreen, Netherlands) in 1.5 ml of nitrate free minimal salts medium (0.1 g/l NaCl; 0.4 g/l NH 4 Cl; 0.1 g/l CaCl 2 *H 2 O; 1.0 g/l MgSO 4 *7H 2 O; 10.0 g/l sucrose; 5.41 g/l hydroxyapatite).
- the plates were inoculated from glycerol spore suspension stock plates stored at 80° C. using a 96-pin cryo-replicator that was heat sterilized and cooled before transfer.
- the filtered supernatants were diluted 100 ⁇ with sterile water and soluble phosphate was measured using a plate reading spectrophotometer (Biotek, Winooski, Vt.) with the BioVision Phosphate Colorimetric Assay Kit (BioVision Research Products, Mountain View, Calif.) and accompanying instructions. Each isolate was grown and tested for phosphate solubilization in triplicate and the averages and standard deviations were calculated. Six of the isolates exhibited enhanced phosphate-solubilization as compared to Penicillium bilaiae ATCC-20851.
- Acinetobacter, Actinomycetes, Aegerita, Agrobacterium e.g., A. radiobacter strains such as K1026 and K84
- Akanthomyces Alcaligenes, Alternaria
- Aminobacter e.g., A. aganoensis, A. aminovorans, A. anthyllidis, A. ciceronei, A. lissarensis, A. niigataensis
- Ampelomyces e.g., A. quisqualis strains such as M-10
- Anabaena e.g., A. aequalis, A. affinis, A. angstumalis angstumalis, A.
- flavus strains such as NRRL 21882, A. parasiticus
- Aulosira e.g., A. aenigmatica, A. africana, A. bohemensis, A. bombayensis, A. confluens, A. fertilissima, A. fertilissma var. tenius, A. fritschii, A. godoyana, A. implexa, A. laxa, A. plantonica, A. prolfica, A. pseuodoramosa, A. schauinslandii, A. striata, A. terrestris, A. thermalis ), Aureobacterium, Aureobasidium (e.g., A.
- pullulans strains such as DSM 14940 and DSM 14941), Azobacter, Azorhizobium (e.g., A. caulinodans, A. doebereinerae, A. oxalatiphilum ), Azospirillum (e.g., A. amazonense strains such as BR 11140 (SpY2T), A. brasilense strains such as INTA Az-39, AZ39, XOH, BR 11002, BR 11005, Ab-V5 and Ab-V6 , A. canadense, A. doebereinerae, A. formosense, A. halopraeferans, A. irakense, A. largimobile, A.
- Azobacter e.g., A. caulinodans, A. doebereinerae, A. oxalatiphilum
- Azospirillum e.g., A. amazonense strains such as BR 11140
- lipoferum strains such as BR 11646, A. melinis, A. oryzae, A. picis, A. rugosum, A. thiophilum, A. zeae ), Azotobacter (e.g., A. agilis, A. armeniacus , A. sp. AR, A. beijerinckii, A. chroococcum , A. DCU26, A. FA8, A. nigricans, A. paspali, A. salinestris, A. tropicalis, A. vinelandii ), Bacillus (e.g., B.
- Azotobacter e.g., A. agilis, A. armeniacus , A. sp. AR, A. beijerinckii, A. chroococcum , A. DCU26, A. FA8, A. nigricans, A. paspali, A. salinestris, A. tropicalis, A
- amyloliquefaciens strains such as D747, NRRL B-50349, TJ1000 (also known as 1BE, isolate ATCC BAA-390), FZB24, FZB42, IN937a, IT-45, TJ1000, MBI600, BS27 (deposited as NRRL B-5015), BS2084 (deposited as NRRL B-50013), 15AP4 (deposited as ATCC PTA-6507), 3AP4 (deposited as ATCC PTA-6506), LSSA01 (deposited as NRRL B-50104), ABP278 (deposited as NRRL B-50634), 1013 (deposited as NRRL B-50509), 918 (deposited as NRRL B-50508), 22CP1 (deposited as ATCC PTA-6508) and BS18 (deposited as NRRL B-50633), B.
- TJ1000 also known as 1BE, isolate ATCC BAA-390
- B. cereus strains such as I-1562, B. firmus strains such as I-1582, B. laevolacticus, B. lichenformis strains such as BA842 (deposited as NRRL B-50516) and BL21 (deposited as NRRL B-50134), B. macerns, B. firmus, B. mycoides strains such as NRRL B-21664, B. pasteurii, B. pumilus strains such as NRRL B-21662, NRRL B-30087, ATCC 55608, ATCC 55609, GB34, KFP9F and QST 2808, B. sphaericus, B.
- subtilis strains such as ATCC 55078, ATCC 55079, MBI 600, NRRL B-21661, NRRL B-21665, CX-9060, GB03, GB07, QST 713, FZB24, D747 and 3BP5 (deposited as NRRL B-50510), B.
- thuringiensis strains such as ATCC 13367, GC-91, NRRL B-21619, ABTS-1857, SAN 401 I, ABG-6305, ABG-6346, AM65-52, SA-12, SB4, ABTS-351, HD-1, EG 2348, EG 7826, EG 7841, DSM 2803, NB-125 and NB-176), Beijerinckia, Beauveria (e.g., B. bassiana strains such as ATCC 26851, ATCC 48023, ATCC 48585, ATCC 74040, ATCC-74250, DSM 12256 and PPRI 5339), Beijerinckia, Blastodendrion, Bosea (e.g., B.
- Bradyrhizobium e.g., B. arachidis, B. bete, B. canariense, B. cytisi, B. daqingense, B. denitrificans, B. diazoefficiens, B. elkanii strains such as SEMIA 501, SEMIA 587 and SEMIA 5019, B. ganzhouense, B. huanghuauhaiense, B. icense, B. ingae, B.
- Bradyrhizobium e.g., B. arachidis, B. bete, B. canariense, B. cytisi, B. daqingense, B. denitrificans, B. diazoefficiens, B. elkanii strains such as SEMIA 501, SEMIA 587 and SEMIA 5019, B. ganzhouense, B. huanghuauhaiense, B. icense, B. ingae, B.
- NRRL B-50586 also deposited as NRRL B-59565
- NRRL B-50587 also deposited as NRRL B-59566
- NRRL B-50588 also deposited as NRRL B-59567
- NRRL B-50589 also deposited as NRRL B-59568
- NRRL B-50590 also deposited as NRRL B-59569
- NRRL B-50591 also deposited as NRRL B-59570
- NRRL B-50592 also deposited as NRRL B-59571
- NRRL B-50593 also deposited as NRRL B-59572
- NRRL B-50594 also deposited as NRRL B-50493
- NRRL B-50608 NRRL B-50609, NRRL B-50610, NRRL B-50611, NRRL B-50612, NRRL B-50726, NRRL B-50727, NRRL B-5072
- jicamae B. lablabi, B. liaoningense, B. manausense, B. neotropicale, B. oligotrophicum, B. ottawaense, B. pachyrhizi, B. paxllaeri, B. retamae, B. rifense, B. valentinum, B. yuanmingense ), Burkholderia (e.g., B. acidipaludis, B. ambifaria, B. andropogonis, B. anthina, B. arboris, B. bannensis, B. bryophila, B. caledonica, B. caribensis, B. caryophylli, B.
- Burkholderia e.g., B. acidipaludis, B. ambifaria, B. andropogonis, B. anthina, B. arboris, B. bannensis, B. bryophila, B.
- cenocepacua B. choica, B. cocovenenans, B. contaminans, B. denitrificans, B. diazotrophica, B. diffusa, B. dilworthii, B. dolosa, B. eburnea, B. endofungorum, B. ferrariae, B. fungorum, B. ginsengisoli, B. gladioli, B. glathei, B. glumae, B. graminis, B. grimmiae, B. heleia, B. hospital, B. humi, B. kururiensis, B. lata, B. latens, B. mallei, B. megapolitana, B. metallica, B.
- mimosarum B. multivorans, B. nodosa, B. norimbergensis, B. oklahomensis, B. phenazinium, B. phenoliruptrix, B. phymatum, B. phytofirmans, B. pickettii, B. plantarii, B. pseudomallei, B. pseudomultivorans, B. pyrrocinia, B. rhizoxinica, B. rhynchosiae, B. sabiae, B. sacchari, B. sartisoli, B. sediminicola, B. seminalis, B. silvatlantica, B. singaporensis, B. soli, B. sordidcola , B.
- sp. strains such as A396, B. sprentiae, B. stabilis, B. symbiotica, B. telluris, B. terrae, B. terrestris, B. terricola, B. thailandensis, B. tropica, B. tuberum, B. ubonensis, B. udeis, B. unamae, B. vandii, B. vietnamiensis, B. xenovorans, B. zhejiangensis ), Brevibacillus, Burkholderia (e.g., B. sp. A396 nov.
- Burkholderia e.g., B. sp. A396 nov.
- rinojensis NRRL B-50319 Calonectria
- Candida e.g., C. oleophila such I-182, C. saitoana
- Candidatus e.g., C. Burkholderia calva, C. Burkholderia crenata, C. Burkholderia hispidae, C. Burkholderia kirkii, C. Burkholderia mamillata, C. Burkholderia nigropunctata, C. Burkholderia rigidae, C. Burkholderia schumannianae, C. Burkholderia strigens, C.
- Candida e.g., C. oleophila such I-182, C. saitoana
- Candidatus e.g., C. Burkholderia calva, C. Burkholderia crenata, C. Burkholderia hispidae, C. Burkholderia kirkii,
- Phytoplasma allocasuarinae C. Phytoplasma americanum, C. Phytoplasma asteris, C. Phytoplasma aurantifolia, C. Phytoplasma australiense, C. Phytoplasma balanitae, C. Phytoplasma brasiliense, C. Phytoplasma caricae, C. Phytoplasma castaneae, C. Phytoplasma cocosnigeriae, C. Phytoplasma cocostanzaniae, C. Phytoplasma convolvuli, C. Phytoplasma costaricanum, C. Phytoplasma cynodontis, C. Phytoplasma fragariae, C.
- Phytoplasma fraxini C. Phytoplasma graminis, C. Phytoplasma japonicum, C. Phytoplasma luffae, C. Phytoplasma lycopersici, C. Phytoplasma malasianum, C. Phytoplasma mali, C. Phytoplasma omanense, C. Phytoplasma oryzae, C. Phytoplasma palmae, C. Phytoplasma palmicola, C. Phytoplasma phoenicium, C. Phytoplasma pini, C. Phytoplasma pruni, C. Phytoplasma prunorum, C. Phytoplasma pyri, C.
- Phytoplasma rhamni C. Phytoplasma rubi, C. Phytoplasma solani, C. Phytoplasma spartii, C. Phytoplasma sudamericanum, C. Phytoplasma tamaricis, C. Phytoplasma trifolii, C. Phytoplasma ulmi, C. Phytoplasma vitis, C. Phytoplasma ziziphi ), Chromobacterium (e.g., C. subtsugae NRRL B-30655 and PRAA4-1, C. vaccinia strains such as NRRL B-50880, C.
- Chromobacterium e.g., C. subtsugae NRRL B-30655 and PRAA4-1, C. vaccinia strains such as NRRL B-50880, C.
- Clonostachys e.g., C. rosea f. catenulata (also referred to as Gliocladium catenulatum ) strains such as J1446)
- Clostridium e.g., Coelemomyces, Coelomycidium, Colletotrichum (e.g., C. gloeosporioides strains such as ATCC 52634)
- Comomonas Conidiobolus
- Coniothyrium e.g., C.
- minitans strains such as CON/M191-08), Cordyceps, Corynebacterium, Couchia, Cryphonectria (e.g., C. parasitica ), Cryptococcus (e.g., C. albidus ), Cryptophlebia (e.g., C. leucotreta ), Culicinomyces, Cupriavidus (e.g., C. alkaliphilus, C. basilensis, C. campinensis, C. gilardii, C. laharis, C. metallidurans, C. numazuensis, C. oxalaticus, C. pampae, C. pauculus, C.
- Cryphonectria e.g., C. parasitica
- Cryptococcus e.g., C. albidus
- Cryptophlebia e.g., C. leucotreta
- Culicinomyces e.g.,
- Cydia e.g., C. pomonella strains such as V03 and V22
- Dactylaria e.g., D. candida
- Delftia e.g., D. acidovorans strains such as RAY209
- Desulforibtio Desulfovibrio
- Devosia e.g., D. neptuniae
- Dilophosphora e.g., D.
- alopecuri Engyodontium, Enterobacter, Entomophaga, Entomophthora, Erynia, Escherichia (e.g., E. intermedia ), Eupenicillium, Exiguobacaterium, Filariomyces, Filobasidiella, Flavobacterium (e.g., F. H492 NRRL B-50584), Frankia (e.g., F. alni ), Fusarium (e.g., F. laterium, F. oxysporum, F. solani ), Gibellula, Gigaspora (e.g., G. margarita ), Gliocladium (e.g., G.
- virens strains such as ATCC 52045 and GL-21
- Glomus e.g., G. aggregatum, G. brasilianum, G. clarum, G. deserticola, G. etunicatum, G. fasciculatum, G. intraradices strains such as RTI-801, G. monosporum, G. mosseae
- Gluconobacter Halospirulina, Harposporium (e.g., H. anguillulae ), Hesperomyces, Hirsutella (e.g., H. minnesotensis, H. rhossiliensis, H.
- thomsonii strains such as ATCC 24874), Hydrogenophage, Hymenoscyphous (e.g., H. ericae ), Hymenostilbe, Hypocrella, Isaria (e.g., I. fumosorosea strains such as Apopka-97 (deposited as ATCC 20874)), Klebsiella (e.g., K. pneumoniae, K. oxytoca ), Kluyvera, Laccaria (e.g., L. bicolor , L. laccata ), Lactobacillus, Lagenidium, Lecanicillium (e.g., L. lecanii strains such as KVO1 , L.
- longisporum strains such as KV42 and KV71
- Leptolegnia Lysobacter (e.g., L. antibioticus strains such as 13-1 and HS124 , L. enzymogenes strains such as 3.1T8)
- Massospora Meristacrum (e.g., M. asterospermum )
- Mesorhizobium e.g., M. abyssinicae, M. albiziae, M. alhagi, M. amorphae, M. australicum, M. camelthorni, M. caraganae, M. chacoense, M. ciceri, M. gobiense, M. hawassense, M.
- M. loti M. mediterraneum
- M. metallidurans M. muleiense
- M. opportunistum M. plurifarium
- M. qingshengii M. robiniae
- M. sangaii M. septentrionale
- M. shangrilense M. shonense
- M. silamurunense M. tamadayense
- M. tarimense M. temperatum
- M. thiogangeticum M. tianshanense
- Metarhizium e.g., M. anisopliae (also referred to as M.
- M. flavoviride strains such as ATCC 32969), Methylobacterium (e.g., M. adhaesivum, M. aerolatum, M. aminovorans, M. aquaticum, M. brachiatum, M. brachythecii, M. bullatum, M. cerastii, M. chloromethanicum, M.ticianookense, M.
- dichloromethanicum M. extorquens, M. fujisawaense, M. gnaphalii, M. goesingense, M. gossipiicola, M. gregans, M. haplocladii, M. hispanicum, M. iners, M. isbiliense, M. jeotgali, M. komagatae, M. longum, M. lusitanum, M. marchantiae, M. mesophilicum, M. nodulans, M. organophilum, M. oryzae, M. oxalidis, M. persicinum, M. phyllosphaerae, M. platani, M. podarium, M. populi, M.
- radiotolerans M. rhodesianum, M. rhodinum, M. salsuginis, M. soli, M. suomiense, M. tardum, M. tarhaniae, M. thiocyanatum, M. thurigiense, M. trifolii, M. variabile, M. zatmanii ), Metschnikowia (e.g., M. fructicola ), Microbacterium (e.g., M. laevaniformans ), Microdochium (e.g., M. dimerum ), Microsphaeropsis (e.g., M. ochracea P130A), Microvirga (e.g., M. aerilata, M.
- Nomuraea e.g., N. rileyi strains such as SA86101, GU87401, SR86151, CG128 and VA9101
- Nostoc e.g., N. azollae, N. caeruleum, N. carneum, N. comminutum, N. commune, N. ellipsosporum, N. flagelliforme, N. linckia, N. longstaffi, N. microscopicum, N. muscorum, N. paludosum, N. pruniforme, N. punctifrome, N. sphaericum, N. sphaeroides, N.
- Ochrobactrum e.g., O. anthropi, O. cicero, O. cytisi, O. daejeonense, O. gallinmfaecis, O. grigonense, O. guangzhouense, I. haematophilum, O. intermedium, O. lupini, O. oryzae, O. pectoris, O. pituitosum, O. pseudointermedium, I. pseudogrignonense, O. rhizosphaerae, O. thiophenivorans, O. tritici ), Oidiodendron, Paecilomyces (e.g., P.
- fumosoroseus strains such as FE991 and FE 9901, P. lilacinus strains such as 251, DSM 15169 and BCP2), Paenibacillus (e.g., P. alvei strains such as NAS6G6, P. azotofixans, P. polymyxa strains such as ABP166 (deposited as NRRL B-50211)), Pandora, Pantoea (e.g., P. agglomerans strains such as NRRL B-21856, P. vagans strains such as C9-1), Paraglomus (e.g., P. brazilianum ), Paraisaria, Pasteuria, Pasteuria (e.g., P.
- nishizawae strains such as Pn1 , P. penetrans, P. ramose , P. sp. strains such as ATCC PTA-9643 and ATCC SD-5832 , P. thornea, P. usage ), Penicillium (e.g., P. albidum, P. aurantiogriseum, P. bilaiae (formerly known as P. bilaii and P.
- bilaji bilaji ) strains such as ATCC 18309, ATCC 20851, ATCC 22348, NRRL 50162, NRRL 50169, NRRL 50776, NRRL 50777, NRRL 50778, NRRL 50777, NRRL 50778, NRRL 50779, NRRL 50780, NRRL 50781, NRRL 50782, NRRL 50783, NRRL 50784, NRRL 50785, NRRL 50786, NRRL 50787, NRRL 50788 and RS7B-SD1 , P. brevicompactum strains such as AgRF18, P. canescens strains such as ATCC 10419 , P. chyrsogenum, P.
- P. brevicompactum strains such as AgRF18, P. canescens strains such as ATCC 10419 , P. chyrsogenum, P.
- citreonigrum P. citrinum, P. digitatum, P. expansum strains such as ATCC 24692 and YT02, P. fellatanum strains such as ATCC 48694 , P. frequentas, P. fuscum, P. fussiporus, P. gaestrivorus strains such as NRRL 50170, P. glabrum strains such as DAOM 239074 and CBS 229.28, P. glaucum, P. griseofulvum, P. implicatum, P. janthinellum strains such as ATCC 10455 , P. lanosocoeruleum strains such as ATCC 48919 , P. lilacinum, P.
- minioluteum P. montanense, P. nigricans, P. oxalicum, P. pinetorum, P. pinophilum, P. purpurogenum, P. radicum strains such as ATCC 201836, FRR 4717, FRR 4719 and N93/47267 , P. raistrickii strains such as ATCC 10490, P. rugulosum, P. simplicissimum, P. solitum, P. variabile, P. velutinum, P. viridicatum ), Phingobacterium, Phlebiopsis (e.g., P. gigantea ), Photorhabdus, Phyllobacterium (e.g., P. bourgognense, P.
- brassicacearum P. catacumbae, P. endophyticum, P. ifriqiyense, P. leguminum, P. loti, P. myrsinacearum, P. sophorae, P. trifolii ), Pichia (e.g., P. anomala strains such as WRL-076), Pisolithus (e.g., P. tinctorius ), Planktothricoides, Plectonema, Pleurodesmospora, Pochonia (e.g., P. chlamydopora ), Podonectria, Polycephalomyces, Prochlorocoous (e.g., P.
- Prochloron e.g., P. didemni
- Prochlorothrix Pseudogibellula
- Pseudomonas e.g., P. agarici , P. antartica, P. aurantiaca, P. aureofaciens, P. azotifigens, P. azotoformans, P. balearica, P. blatchfordae, P. brassicacearum, P. brenneri, P. cannabina, P. cedrina, P. cepacia, P. chlororaphis strains such as MA 342, P. congelans, P. corrugata, P. costantinii, P.
- mucidolens P. orientalis, P. oryzihabitans, P. palleroniana, P. panacis, P. parafulva, P. peli, P. pertucinogena, P. plecoglossicida, P. protogens, P. proteolytica, P. putida, P. pyrocina strains such as ATCC 15958, P. rhodesiae , P. sp. strains such as DSM 13134 , P. striata, P. stutzeri, P. syringae, P. synxantha, P. taetrolens, P. thisvervalensis, P. tolaasii, P.
- Pseudozyma e.g., P. flocculosa strains such as PF-A22 UL
- Pythium e.g., P. oligandrum strains such as DV 74
- Rhizobium e.g., R. aggregatum, R. alamii, R. alkalisoli, P. alvei, P. azibense, P. borbori, R. calliandrae, R. cauense, R. cellulosilyticum, R. daejeonense, R. endolithicum, R. endophyticum, R. etli, R. fabae, R. flavum, R. fredii, R.
- Rhizobium e.g., R. aggregatum, R. alamii, R. alkalisoli, P. alvei, P. azibense, P. borbori, R. calliandrae, R. cauense, R. cell
- Rhizoctonia Rhizopogon (e.g., R. amylopogon, R. fulvigleba, R. luteolus, R. villosuli ), Rhodococcus , Saccharopolyspora (e.g., S. spinosa ), Scleroderma (e.g., S. cepa S. citrinum ), Septobasidium, Serratia , Shinella (e.g., S. kummerowiae ), Sinorhizoium (e.g., S. abri, S. adhaerens, S. americanum, S. arboris, S.
- S. fredii strains such as CCBAU114 and USDA 205 , S. garamanticus, S. indiaense, S. kostiense, S. kummerowiae, S. medicae, S. meliloti strains such as MSDJ0848, S. mexicanus, S. numidicus, S. psoraleae, S. saheli, S. sesbaniae, S. sojae, S. terangae, S. xinjiangense ), Sorosporella, Sphaerodes (e.g., S.
- mycoparasitica strains such as IDAC 301008-01), Spodoptera (e.g., S. littoralis ), Sporodiniella, Steinernema (e.g., S. carpocapsae, S. feltiae, S. kraussei strains such as L137), Stenotrophomonas, Streptomyces (e.g., S. NRRL B-30145, S. M1064, S. WYE 53 (deposited as ATCC 55750), S. cacaoi strains such as ATCC 19093, S. galbus strains such as NRRL 30232, S. griseoviridis strains such as K61, S.
- lydicus strains such as WYEC 108 (deposited as ATCC 55445), S. violaceusniger strains such as YCED-9 (deposited as ATCC 55660)), Streptosporangium, Stillbella, Swaminathania, Talaromyces (e.g., T. aculeatus, T. flavus strains such as VI17b), Tetranacrium, Thiobacillus, Tilachlidium, Tolypocladium, Tolypothrix, Torrubiella, Torulospora, Trenomyces, Trichoderma (e.g. T. asperellum strains such as SKT-1, T. atroviride strains such as LC52 and CNCM 1-1237, T.
- T. asperellum strains such as SKT-1
- T. atroviride strains such as LC52 and CNCM 1-1237, T.
- oudemansii strains such as HRU3
- Uredinella Variovorax
- Verticillium e.g., V. chlamydosporum, V. lecanii strains such as ATCC 46578
- Vibrio Xanthobacter
- Xanthomonas. Xenorhadbus Yersinia (e.g., Y. entomophaga strains such as 082KB8)
Abstract
The present disclosure provides novel fungal strains capable of solubilizing phosphate, as well as compositions and methods of using those strains to increase the amount/concentration of soluble phosphate in a medium that comprises insoluble phosphate.
Description
- The present application is a divisional of pending U.S. patent application Ser. No. 16/073,050, filed on Jul. 26, 2018, which is a 35 U.S.C. 371 national application of International Patent Application No. PCT/US2017/013416, filed Jan. 13, 2017, which claims priority or the benefit under 35 U.S.C. 119 to U.S. Provisional Patent Application No. 62/287,965, filed Jan. 28, 2016, the contents of each of which are fully incorporated herein by reference.
- This application claims priority to U.S. Provisional Patent Application No. 62/287,965, filed Jan. 28, 2016.
- The present disclosure contains references to biological material deposited under the terms of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure at the Agricultural Research Service Culture Collection, 1815 North University Street, Peoria, Ill. 61604, U.S.A.
- The present disclosure relates to isolated fungal strains and new fungal strains derived therefrom.
- Certain strains of Penicillium are capable of solubilizing phosphate. See, e.g., U.S. Pat. Nos. 5,026,417; 5,484,464; 7,241,588; 8,278,247 and 9,101,088; U.S. Patent Publication Nos. 2014/014909.
- Efforts to isolate and/or create new and improved phosphate-solubilizing Penicillium strains are ongoing.
- A first aspect of the present disclosure is an isolated strain of Penicillium bilaiae selected from the group consisting of the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67154 (P. bilaiae NRRL 67154), the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67155 (P. bilaiae NRRL 67155), the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67156 (P. bilaiae NRRL 67156), the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67157 (P. bilaiae NRRL 67157), the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67158 (P. bilaiae NRRL 67158), or the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67159 (P. bilaiae NRRL 67159).
- A second aspect of the present disclosure is a biologically pure culture of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159.
- A third aspect of the present disclosure is an inoculant composition comprising P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159. In some embodiments, the inoculant composition comprises one or more stabilizing compounds, one or more pesticides, one or more lipo-chitooligosaccharides, one or more chitooligosaccharides, one or more chitinous compounds, one or more flavonoids, and/or one or more additional microorganisms.
- A fourth aspect of the present disclosure is a kit comprising an inoculant composition comprising P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 and a container housing said inoculant composition.
- A fifth aspect of the present disclosure is a method of increasing the amount/concentration of soluble phosphate in a medium that contains insoluble phosphate, said method comprising applying P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 to said medium in an amount/concentration effective to solubilize at least a portion of the insoluble phosphate therein.
- A sixth aspect of the present disclosure is a method of increasing the amount/concentration of soluble phosphate in a medium that contains insoluble phosphate, said method comprising introducing P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 into said medium in an amount/concentration effective to solubilize at least a portion of the insoluble phosphate therein.
- An eighth aspect of the present disclosure is a method of increasing the amount/concentration of soluble phosphate in a medium that contains insoluble phosphate, said method comprising applying a biologically pure culture of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159 to said medium in an amount/concentration effective to solubilize at least a portion of the insoluble phosphate therein.
- A ninth aspect of the present disclosure is a method of increasing the amount/concentration of soluble phosphate in a medium that contains insoluble phosphate, said method comprising introducing the biologically pure culture of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159 into said medium in an amount/concentration effective to solubilize at least a portion of the insoluble phosphate therein.
- A tenth aspect of the present disclosure is a method of increasing the amount/concentration of soluble phosphate in a medium that contains insoluble phosphate, said method comprising applying an inoculant composition comprising P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 to said medium in an amount/concentration effective to solubilize at least a portion of the insoluble phosphate therein.
- An eleventh aspect of the present disclosure is a method of increasing the amount/concentration of soluble phosphate in a medium that contains insoluble phosphate, said method comprising introducing an inoculant composition comprising P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 into said medium in an amount/concentration effective to solubilize at least a portion of the insoluble phosphate therein.
- This description is not intended to be a detailed catalog of all the different ways in which the invention may be implemented or of all the features that may be added to the instant invention. For example, features illustrated with respect to one embodiment may be incorporated into other embodiments and features illustrated with respect to a particular embodiment may be deleted from that embodiment. In addition, numerous variations and additions to the various embodiments suggested herein, which do not depart from the instant invention, will be apparent to those skilled in the art in light of the instant disclosure. Hence, the following description is intended to illustrate some particular embodiments of the invention and not to exhaustively specify all permutations, combinations and variations thereof.
- The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
- Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the specification and relevant art and should not be interpreted in an idealized or overly formal sense unless expressly so defined herein. For the sake of brevity and/or clarity, well-known functions or constructions may not be described in detail.
- As used herein, the singular forms “a,” “an,” and “the” are intended to include the plural forms as well, unless the context clearly indicates otherwise.
- As used herein, the terms “acaricide” and “acaricidal” refer to an agent or combination of agents the application of which is toxic to an acarid (i.e., kills an acarid, inhibits the growth of an acarid and/or inhibits the reproduction of an acarid).
- As used herein, the term “and/or” is intended to include any and all combinations of one or more of the associated listed items, as well as the lack of combinations when interpreted in the alternative (“or”).
- As used herein, the term “aqueous” refers to a composition that contains more than a trace amount of water (i.e., more than 0.5% water by weight, based upon the total weight of the composition).
- As used herein, the term “biologically pure culture” refers to a microbial culture that is free or essentially free of biological contamination and that has genetic uniformity such that different subcultures taken therefrom will exhibit identical or substantially identical genotyopes and phenotypes. In some embodiments, the biologically pure culture is 100% pure (i.e., all subcultures taken therefrom exhibit identical genotypes and phenotypes). In some embodiments, the biologically pure culture is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 99.5, 99.6, 99.7, 99.8, or 99.9% pure (i.e., at least 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 99.5, 99.6, 99.7, 99.8, or 99.9% of the subcultures taken therefrom exhibit identical genotypes and phenotypes).
- As used herein, the term “BRADY” is to be interpreted as a shorthand substitute for the phrase “Bradyrhizobium elkanii SEMIA 501, Bradyrhizobium elkanii SEMIA 587, Bradyrhizobium elkanii SEMIA 5019, Bradyrhizobium japonicum NRRL B-50586 (also deposited as NRRL B-59565), Bradyrhizobium japonicum NRRL B-50587 (also deposited as NRRL B-59566), Bradyrhizobium japonicum NRRL B-50588 (also deposited as NRRL B-59567), Bradyrhizobium japonicum NRRL B-50589 (also deposited as NRRL B-59568), Bradyrhizobium japonicum NRRL B-50590 (also deposited as NRRL B-59569), Bradyrhizobium japonicum NRRL B-50591 (also deposited as NRRL B-59570), Bradyrhizobium japonicum NRRL B-50592 (also deposited as NRRL B-59571), Bradyrhizobium japonicum NRRL B-50593 (also deposited as NRRL B-59572), Bradyrhizobium japonicum NRRL B-50594 (also deposited as NRRL B-50493), Bradyrhizobium japonicum NRRL B-50608, Bradyrhizobium japonicum NRRL B-50609, Bradyrhizobium japonicum NRRL B-50610, Bradyrhizobium japonicum NRRL B-50611, Bradyrhizobium japonicum NRRL B-50612, Bradyrhizobium japonicum NRRL B-50726, Bradyrhizobium japonicum NRRL B-50727, Bradyrhizobium japonicum NRRL B-50728, Bradyrhizobium japonicum NRRL B-50729, Bradyrhizobium japonicum NRRL B-50730, Bradyrhizobium japonicum SEMIA 566, Bradyrhizobium japonicum SEMIA 5079, Bradyrhizobium japonicum SEMIA 5080, Bradyrhizobium japonicum USDA 6, Bradyrhizobium japonicum USDA 110, Bradyrhizobium japonicum USDA 122, Bradyrhizobium japonicum USDA 123, Bradyrhizobium japonicum USDA 127, Bradyrhizobium japonicum USDA 129 and/or Bradyrhizobium japonicum USDA 532C.”
- As used herein, the terms “colony forming unit” and “cfu” refer to a microbial cell/spore capable of propagating on or in a suitable growth medium or substrate when conditions (e.g., temperature, moisture, nutrient availability, pH, etc.) are favorable for germination and/or microbial growth.
- As used herein, the term “consists essentially of,”, when used in reference to inoculant compositions and methods of the present disclosure, means that the compositions/methods may contain additional components/steps so long as the additional components/steps do not materially alter the composition/method. The term “materially alter,” as applied to a composition/method of the present disclosure, refers to an increase or decrease in the effectiveness of the composition/method of at least 20%. For example, a component added to an inoculant composition of the present disclosure may be deemed to “materially alter” the composition if it increases or decreases the composition's ability to solubilize phosphate by at least 20%.
- As used herein, the term “diazotroph” refers to an organism capable of converting atmospheric nitrogen (N2) into a form that may be utilized by a plant or plant part (e.g., ammonia (NH3), ammonium (NH4+), etc.).
- As used herein, the term “dispersant” refers to an agent or combination of agents the application of which reduces the cohesiveness of like particles, the surface tension of a liquid, the interfacial tension between two liquids and/or the interfacial tension between or a liquid and a solid.
- As used herein, the terms “effective amount,” “effective concentration” and “effective amount/concentration” refer to an amount or concentration that is sufficient to cause a desired effect (e.g., enhanced phosphate availability). The absolute value of the amount/concentration that is sufficient to cause the desired effect may be affected by factors such as the type and magnitude of effect desired, the type, size and volume of material to which the inoculant composition will be applied, the type(s) of microorganisms in the composition, the number of microorganisms in the composition, the stability of the microorganism(s) in the inoculant composition and the storage conditions (e.g., temperature, relative humidity, duration). Those skilled in the art will understand how to select an effective amount/concentration using routine dose-response experiments.
- As used herein, the term “enhanced dispersion” refers to an improvement in one or more characteristics of microbial dispersion as compared to one or more controls (e.g., a control composition that is identical to an inoculant composition of the present disclosure except that it lacks one or more of the components found in the inoculant composition of the present disclosure). Exemplary microbial dispersion characteristics include, but are not limited to, the percentage of microbes that exist as single cells/spores when the inoculant composition is diluted in water. An inoculant composition that improves one or more microbial dispersion characteristics of the microorganism(s) contained therein as compared to a control composition (e.g., a control composition that is identical to the inoculant composition except that it lacks one or more of the components found in the inoculant composition) provides enhanced dispersion and can be referred to as a “readily dispersable inoculant composition.”
- As used herein, the terms “enhanced stability” and “enhanced microbial stability” refer to an improvement in one or more characteristics of microbial stability as compared to one or more controls (e.g., a control composition that is identical to an inoculant composition of the present disclosure except that it lacks one or more of the components found in the inoculant composition of the present disclosure). Exemplary microbial stability characteristics include, but are not limited to, the ability to germinate and/or propagate after being stored for a defined period of time and the ability to cause a desired effect (e.g., increased phosphate solubility) after being stored for a defined period of time. A microorganism that exhibits improvement in one or more microbial stability characteristics as compared to a control microorganism when each is subjected to the same conditions (e.g., storage conditions) displays enhanced stability and can be referred to as a “stable microorganism.” An inoculant composition that improves one or more microbial stability characteristics of the microorganism(s) contained therein as compared to a control composition (e.g., a control composition that is identical to the inoculant composition except that it lacks one or more of the components found in the inoculant composition) provides enhanced stability and can be referred to as a “stable inoculant composition.”
- As used herein, the terms “enhanced survival” and “enhanced microbial survival” refer to an improvement in the survival rate of one or more microorganisms in an inoculant composition as compared to one or more microorganisms in a control composition (e.g., a control composition that is identical to an inoculant composition of the present disclosure except that it lacks one or more of the components found in the inoculant composition of the present disclosure). An inoculant composition that improves the survival rate of one or more of the microorganisms contained therein as compared to a control composition (e.g., a control composition that is identical to the inoculant composition except that it lacks one or more of the components found in the inoculant composition) provides enhanced survival and can be referred to as a stable inoculant composition.
- As used herein, the terms “fungicide” and “fungicidal” refer to an agent or combination of agents the application of which is toxic to a fungus (i.e., kills a fungus, inhibits the growth of a fungus and/or inhibits the reproduction of a fungus).
- As used herein, the term “fulvic acid” encompasses pure fulvic acids and fulvic acid salts (fulvates). Non-limiting examples of fulvic acids include ammonium fulvate, boron fulvate, potassium fulvate, sodium fulvate, etc. In some embodiments, the fulvic acid comprises, consists essentially of or consists MDL Number MFCD09838488 (CAS Number 479-66-3).
- As used herein, the terms “herbicide” and “herbicidal” refer to an agent or combination of agents the application of which is toxic to a weed (i.e., kills a weed, inhibits the growth of a weed and/or inhibits the reproduction of a weed).
- As used herein, the term “humic acid” encompasses pure humic acids and humic acid salts (humates). Non-limiting examples of humic acids include ammonium humate, boron humate, potassium humate, sodium humate, etc. In some embodiments, the humic acid comprises, consists essentially of or consists of one or more of MDL Number MFCD00147177 (CAS Number 1415-93-6), MDL Number MFCD00135560 (CAS Number 68131-04-4), MDL Number MFCS22495372 (CAS Number 68514-28-3), CAS Number 93924-35-7 and CAS Number 308067-45-0.
- As used herein, the terms “inoculant composition” and “inoculum” refer to a composition comprising microbial cells and/or spores, said cells/spores being capable of propagating/germinating on or in a suitable growth medium or substrate when conditions (e.g., temperature, moisture, nutrient availability, pH, etc.) are favorable for germination and/or microbial growth.
- As used herein, the terms “insecticide” and “insecticidal” refer to an agent or combination of agents the application of which is toxic to an insect (i.e., kills an insect, inhibits the growth of an insect and/or inhibits the reproduction of an insect).
- As used herein, the term “isolated microbial strain” refers to a microbe that has been removed from the environment in which it is normally found.
- As used herein, the term “isomer” includes all stereoisomers of the compounds and/or molecules to which it refers, including enantiomers and diastereomers, as well as all conformers, roatmers and tautomers, unless otherwise indicated. Compounds and/or molecules disclosed herein include all enantiomers in either substantially pure levorotatory or dextrorotatory form, or in a racemic mixture, or in any ratio of enantiomers. Where embodiments disclose a (D)-enantiomer, that embodiment also includes the (L)-enantiomer; where embodiments disclose a (L)-enantiomer, that embodiment also includes the (D)-enantiomer. Where embodiments disclose a (+)-enantiomer, that embodiment also includes the (−)-enantiomer; where embodiments disclose a (−)-enantiomer, that embodiment also includes the (+)-enantiomer. Where embodiments disclose a (S)-enantiomer, that embodiment also includes the (R)-enantiomer; where embodiments disclose a (R)-enantiomer, that embodiment also includes the (S)-enantiomer. Embodiments are intended to include any diastereomers of the compounds and/or molecules referred to herein in diastereomerically pure form and in the form of mixtures in all ratios. Unless stereochemistry is explicitly indicated in a chemical structure or chemical name, the chemical structure or chemical name is intended to embrace all possible stereoisomers, conformers, rotamers and tautomers of compounds and/or molecules depicted.
- As used herein, the term “modified microbial strain” refers to a microbial strain that is modified from a strain isolated from nature. Modified microbial strains may be produced by any suitable method(s), including, but not limited to, chemical or other form of induced mutation to a polynucleotide within any genome within the strain; the insertion or deletion of one or more nucleotides within any genome within the strain, or combinations thereof; an inversion of at least one segment of DNA within any genome within the strain; a rearrangement of any genome within the strain; generalized or specific transduction of homozygous or heterozygous polynucleotide segments into any genome within the strain; introduction of one or more phage into any genome of the strain; transformation of any strain resulting in the introduction into the strain of stably replicating autonomous extrachromosomal DNA; any change to any genome or to the total DNA composition within the strain isolated from nature as a result of conjugation with any different microbial strain; and any combination of the foregoing. The term modified microbial strains includes a strain with (a) one of more heterologous nucleotide sequences, (b) one or more non-naturally occurring copies of a nucleotide sequence isolated from nature (i.e., additional copies of a gene that naturally occurs in the microbial strain from which the modified microbial strain was derived), (c) a lack of one or more nucleotide sequences that would otherwise be present in the natural reference strain by for example deleting nucleotide sequence, and (d) added extrachromosomal DNA. In some embodiments, modified microbial strains comprise a combination of two or more nucleotide sequences (e.g., two or more naturally occurring genes that do not naturally occur in the same microbial strain) or comprise a nucleotide sequence isolated from nature at a locus that is different from the natural locus.
- As used herein, the terms “nematicide” and “nematicidal” refer to an agent or combination of agents the application of which is toxic to a nematode (i.e., kills a nematode, inhibits the growth of a nematode and/or inhibits the reproduction of a nematode).
- As used herein, the term “nitrogen fixing organism” refers to an organism capable of converting atmospheric nitrogen (N2) into a form that may be utilized by a plant or plant part (e.g., ammonia (NH3), ammonium (NH4+), etc.).
- As used herein, the term “non-aqueous” refers to a composition that comprises no more than a trace amount of water (i.e., no more than 0.5% water by weight, based upon the total weight of the composition).
- As used herein, the term “PENI” is to be interpreted as a shorthand substitute for the phrase “Penicillium bilaiae ATCC 18309, Penicillium bilaiae ATCC 20851, Penicillium bilaiae ATCC 22348, Penicillium bilaiae NRRL 50162, Penicillium bilaiae NRRL 50169, Penicillium bilaiae NRRL 50776, Penicillium bilaiae NRRL 50777, Penicillium bilaiae NRRL 50778, Penicillium bilaiae NRRL 50777, Penicillium bilaiae NRRL 50778, Penicillium bilaiae NRRL 50779, Penicillium bilaiae NRRL 50780, Penicillium bilaiae NRRL 50781, Penicillium bilaiae NRRL 50782, Penicillium bilaiae NRRL 50783, Penicillium bilaiae NRRL 50784, Penicillium bilaiae NRRL 50785, Penicillium bilaiae NRRL 50786, Penicillium bilaiae NRRL 50787, Penicillium bilaiae NRRL 50788, Penicillium bilaiae RS7B-SD1, Penicillium brevicompactum AgRF18, Penicillium canescens ATCC 10419, Penicillium expansum ATCC 24692, Penicillium expansum YT02, Penicillium fellatanum ATCC 48694, Penicillium gaestrivorus NRRL 50170, Penicillium glabrum DAOM 239074, Penicillium glabrum CBS 229.28, Penicillium janthinellum ATCC 10455, Penicillium lanosocoeruleum ATCC 48919, Penicillium radicum ATCC 201836, Penicillium radicum FRR 4717, Penicillium radicum FRR 4719, Penicillium radicum N93/47267 and/or Penicillium raistrickii ATCC 10490.”
- As used herein, the term “Penicillium bilaiae” is intended to include all iterations of the species name, such as “Penicillium bilaji” and “Penicillium bilaii.”
- As used herein, the terms “percent identity,” “% identity” and “percent identical” refer to the relatedness of two or more nucleotide or amino acid sequences, which may be calculated by (i) comparing two optimally aligned sequences over a window of comparison, (ii) determining the number of positions at which the identical nucleic acid base (for nucleotide sequences) or amino acid residue (for proteins) occurs in both sequences to yield the number of matched positions, (iii) dividing the number of matched positions by the total number of positions in the window of comparison, and then (iv) multiplying this quotient by 100% to yield the percent identity. If the “percent identity” is being calculated in relation to a reference sequence without a particular comparison window being specified, then the percent identity is determined by dividing the number of matched positions over the region of alignment by the total length of the reference sequence. Accordingly, for purposes of the present invention, when two sequences (query and subject) are optimally aligned (with allowance for gaps in their alignment), the “percent identity” for the query sequence is equal to the number of identical positions between the two sequences divided by the total number of positions in the query sequence over its length (or a comparison window), which is then multiplied by 100%.
- As used herein, the terms “pesticide” and “pesticidal” refer to agents or combinations of agents the application of which is toxic to a pest (i.e., kills a pest, inhibits the growth of a pest and/or inhibits the reproduction of a pest). Non-limiting examples of pesticides include acaricides, fungicides, herbicides, insecticides, nematicides, rodenticides, virucides, gastropodicides, etc.
- As used herein, the term “phosphate-solubilizing microorganism” refers to a microorganism capable of converting insoluble phosphate into a soluble form of phosphate.
- As used herein, the term “progeny” refers to the descendent(s) of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 and encompasses both immediate offspring of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 and any decendants thereof.
- As used herein, the terms “spore” and “microbial spore” refer to a microorganism in its dormant, protected state.
- As used herein, the term “stabilizing compound” refers to an agent or combination of agents the application of which enhances the stability and/or survival of one or more microorganisms in an inoculant composition.
- As used herein with respect to inoculant compositions, the term “stable” refers to an inoculant composition in which microorganisms exhibit enhanced stability and/or enhanced survival. In general, an inoculant composition may be labeled “stable” if it improves the survival rate and/or at least one microbial stability characteristic of at least one microorganism contained therein.
- As used herein with respect to microbial strains, the term “survival rate” refers to the percentage of microbial cell/spore that are viable (i.e., capable of propagating on or in a suitable growth medium or substrate when conditions (e.g., temperature, moisture, nutrient availability, pH, etc.) are favorable for germination and/or microbial growth) at a given period of time.
- While certain aspects of the present disclosure will hereinafter be described with reference to embodiments thereof, it will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the present disclosure as defined by the claims.
- All publications, patent applications, patents and other references mentioned herein are incorporated by reference in their entirety, except insofar as they contradict any disclosure expressly set forth herein.
- The present disclosure provides isolated fungal strains capable of converting insoluble phosphate into a soluble form of phosphate.
- In some embodiments, the isolated fungal strain is P. bilaiae NRRL 67154, P. bilaiae NRRL 67155, P. bilaiae NRRL 67156, P. bilaiae NRRL 67157, P. bilaiae NRRL 67158, or P. bilaiae NRRL 67159.
- The present disclosure extends to the progeny of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 and to modified microbial strains derived from P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159.
- Progeny of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 may be produced using any suitable method(s), including, but not limited to, protoplast fusion, traditional breeding programs (e.g., backcrossing, inbredding, etc.) and combinations thereof. In some embodiments, the progeny is born of a cross between two of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and P. bilaiae NRRL B-67159. In some embodiments, the progeny is born of a cross between one of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and P. bilaiae NRRL B-67159 and another fungal strain capable of converting insoluble phosphate into a soluble form of phosphate. In some embodiments, the progeny is born of a cross between one of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and P. bilaiae NRRL B-67159 and a fungal strain incapable of converting insoluble phosphate into a soluble form of phosphate.
- Modified microbial strains derived from P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 may be produced using suitable method(s), including, but not limited to, chemical or other form of induced mutation to a polynucleotide within any genome within P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159; the insertion or deletion of one or more nucleotides within any genome within P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159, or combinations thereof; an inversion of at least one segment of DNA within any genome within P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159; a rearrangement of any genome within P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159; generalized or specific transduction of homozygous or heterozygous polynucleotide segments into any genome within P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159; introduction of one or more phage into any genome of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159; transformation of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159 resulting in the introduction into P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159 of stably replicating autonomous extrachromosomal DNA; any change to any genome or to the total DNA composition within P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159 as a result of conjugation with any different microbial strain; and any combination of the foregoing.
- Fungal strains of the present disclosure may be cultured using any suitable method(s), including, but not limited to, liquid-state fermentation and solid-state fermentation. See, generally, Cunningham et al., CAN. J. BOT. 68:2270 (1990); Friesen et al., APPL. MICROBIOL. BIOTECH. 68:397 (2005).
- Fungal strains of the present disclosure may be harvested during any suitable growth phase. In some embodiments, the fungal strain is allowed to reach the stationary growth phase prior to harvesting. In some embodiments, the fungal strain is harvested as vegetative cells. In some embodiments, the fungal strain is harvested as spores.
- Fungal strains of the present disclosure may be harvested and/or concentrated using any suitable method(s), including, but not limited to, centrifugation (e.g., density gradient centrifugation, disc stack centrifugation, tubular bowl centrifugation), coagulation, decanting, felt bed collection, filtration (e.g., drum filtration, sieving, ultrafiltration), flocculation, impaction and trapping (e.g., cyclone spore trapping, liquid impingement).
- The present disclosure also provides biologically pure cultures of the fungal strains described herein. In some embodiments, at least 95, 96, 97, 98, 99, 99.5, 99.6, 99.7, 99.8, or 99.9% of subcultures taken from the culture exhibit a genotype that is at least 95, 96, 97, 98, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.55%, 99.6%, 99.65%, 99.7%, 99.75%, 99.8%, 99.85%, 99.9%, 99.95%, or 100% identical to that of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159. In some embodiments, the culture is a biologically pure culture of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, or P. bilaiae NRRL B-67159.
- It is to be understood that cultures of the present invention may comprise vegetative cells and/or dormant spores. According to some embodiments, at least 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98, 99% or more of the microbes in a culture of the present disclosure are present as vegetative cells. According to some embodiments, at least 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98, 99% or more of the microbes in a culture of the present disclosure are present as spores.
- Fungal strains and cultures of the present disclosure may be formulated into any suitable type of composition.
- In some embodiments, the present disclosure provides inoculant compositions comprising one or more fungal strains of the present disclosure in a carrier.
- Fungal strains and cultures of the present disclosure may be incorporated into any suitable inoculant composition, including, but not limited to, inoculant compositions as described in International Patent Application Nos. PCT/US2016/050529 (filed Sep. 7, 2016); PCT/US2016/050647 (filed Sep. 8, 2016); PCT/US2016/067714 (filed Dec. 20, 2016); and PCT/US2016/067745 (filed Dec. 20, 2016); U.S Provisional Patent Nos. 62/343,217 (filed May 31, 2016); 62/347,773 (filed Jun. 9, 2016); 62/347,785 (filed Jun. 9, 2016); 62/347,794 (filed Jun. 9, 2016); 62/347,805 (filed Jun. 9, 2016); and 62/436,529 (filed Dec. 20, 2016).
- Fungal strains of the present disclosure may be incorporated into inoculant compositions in any suitable amount/concentration. The absolute value of the amount/concentration that is/are sufficient to cause the desired effect(s) may be affected by factors such as the type, size and volume of material to which the composition will be applied and storage conditions (e.g., temperature, relative humidity, duration). Those skilled in the art will understand how to select an effective amount/concentration using routine dose-response experiments.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more fungal strains of the present disclosure in an amount ranging from about 1×101 to about 1×1015 colony-forming units (cfu) per gram and/or milliliter of inoculant composition. For example, inoculant compositions of the present disclosure may comprise about 1×101, 1×102, 1×103, 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010, 1×1011, 1×1012, 1×1013, 1×1014, 1×1015 or more cfu of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 cells/spores per gram and/or milliliter of inoculant composition. In some embodiments, inoculant compositions of the present disclosure comprise at least 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010, 1×1011, 1×1012 cfu of one or more fungal strains of the present disclosure per gram and/or milliliter of inoculant composition.
- In some embodiments, cells/spores of one or more fungal strains of the present disclosure comprises about 0.1 to about 95% (by weight) of the inoculant composition. For example, P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 cells/spores may comprise about 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of the inoculant composition. In some embodiments, P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 comprise about 1 to about 25%, about 5 to about 20%, about 5 to about 15%, about 5 to about 10% or about 8 to about 12% (by weight) of the inoculant composition.
- As noted above, and as shown in the Examples below, P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and P. bilaiae NRRL B-67159 are capable of converting insoluble phosphate into a soluble form of phosphate.
- The aforementioned utility is not limited to P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and P. bilaiae NRRL B-67159. Progeny of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 and modified microbial strains derived from P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 may also be capable of solubilizing phosphate. Those skilled in the art will understand how to determine whether a given strain is capable of solubilizing phosphate using routine experiments.
- Accordingly, in some embodiments, inoculant compositions of the present disclosure comprise one or more phosphate-solubilizing progeny of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 (e.g., an elite phosphate-solubilizing inbred strain having a whole genome sequence that is at 99, 99.1, 99.2, 99.3, 99.4, 99.5, 99.6, 99.7, 99.8 or 99.9% identical to that of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159) and/or one or more phosphate-solubilizing modified microbial strains derived from P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 (e.g., a modified microbial strain that solubilizes phosphate and has a whole genome sequence that is at least 99, 99.1, 99.2, 99.3, 99.4, 99.5, 99.6, 99.7, 99.8 or 99.9% identical to that of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159).
- In some embodiments, inoculant compositions of the present disclosure comprise one or more fungal strains of the present disclosure in an effective amount/concentration for increasing the amount/concentration of soluble phosphate when the inoculant composition is introduced into a medium that comprises insoluble phosphate. In some embodiments, inoculant compositions of the present disclosure comprise one or more fungal strains of the present disclosure in an amount/concentration sufficient to increase the amount/concentration of soluble phosphate by at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 225, 250% or more when the inoculant composition is introduced into the medium. For example, in some embodiments, inoculant compositions of the present disclosure comprise cells/spores of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 in an amount/concentration effective to increase the amount/concentration of soluble phosphate in a medium by at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 225 or 250% when the inoculant composition is introduced into the medium.
- Inoculant compositions of the present disclosure may comprise any suitable carrier(s). Selection of appropriate carrier materials will depend on the intended application(s) and the microorganism(s) present in the inoculant composition. In some embodiments, the carrier material(s) will be selected to provide an inoculant composition in the form of a liquid, gel, slurry, or solid. In some embodiments, the carrier will consist essentially of or consist of one or more stabilizing compounds.
- In some embodiments, the inoculant composition comprises one or more solid carriers. According to some embodiments, the inoculant composition comprises one or more powders (e.g., wettable powders) and/or granules. Non-limiting examples of solid carriers include clays (e.g., attapulgite clays, montmorillonite clay, etc.), peat-based powders and granules, freeze-dried powders, spray-dried powders, spray-freeze-dried powders and combinations thereof.
- In some embodiments, the inoculant composition comprises one or more liquid and/or gel carriers. According to some embodiments, the inoculant composition comprises one or more non-aqueous solvents. According to some embodiments, the inoculant composition comprises one or more aqueous solvents (e.g., water). According to some embodiments, an aqueous solvent, such as water, may be combined with a co-solvent, such as ethyl lactate, methyl soyate/ethyl lactate co-solvent blends (e.g., STEPOSOL™, Stepan), isopropanol, acetone, 1,2-propanediol, n-alkylpyrrolidones (e.g., AGSOLEX™ wetting agents; Ashland, Inc., Covington, Ky.), petroleum based-oils (e.g., AROMATIC™ and SOLVESSO™ fluids; ExxonMobil Chemical Company, Spring, Tex.), isoparrafinic hyydrocarbons (e.g., ISOPAR™ fluids; ExxonMobil Chemical Company, Spring, Tex.), cycloparaffinic hydrocarbons (e.g., NAPPAR™ 6; ExxonMobil Chemical Company, Spring, Tex.), mineral spirits (e.g., VARSOL™; ExxonMobil Chemical Company, Spring, Tex.), and mineral oils (e.g., paraffin oil). According to some embodiments, the inoculant composition comprises one or more inorganic solvents, such as decane, dodecane, hexylether and nonane. According to some embodiments, the inoculant composition comprises one or more organic solvents, such as acetone, dichloromethane, ethanol, hexane, methanol, propan-2-ol and trichloroethylene. Non-limiting examples of liquid/gel carriers include oils (e.g., mineral oil, olive oil, peanut oil, soybean oil, sunflower oil), polyethylene glycols (e.g., PEG 200, PEG 300, PEG 400, etc.), propylene glycols (e.g., PPG-9, PPG-10, PPG-17, PPG-20, PPG-26, etc.), ethoxylated alcohols (e.g., TOMADOL® (Air Products and Chemicals, Inc., Allentown, Pa.), TERGITOL™ 15-S surfactants such as TERGITOL™15-S-9 (The Dow Chemical Company, Midland, Mich.), etc.), isoparrafinic hyydrocarbons (e.g., ISOPAR™, ISOPAR™ L, ISOPAR™ M, ISOPAR™ V; ExxonMobil Chemical Company, Spring, Tex.), pentadecane, polysorbates (e.g. polysorbate 20, polysorbate 40, polysorbate 60, polysorbate 80, etc.), silicones (siloxanes, trisiloxanes, etc.) and combinations thereof.
- Inoculant compositions of the present disclosure may comprise any suitable stabilizing compound(s), including, but not limited to, maltodextrins, monosaccharides, disaccharides, oligosaccharides, sugar alcohols, humic acids, fulvic acids, malt extracts, peat extracts, betaines, prolines, sarcosines, peptones, skim milks, oxidation control components, hygroscopic polymers and UV protectants.
- In some embodiments, the inoculant composition comprises one or more maltodextrins (e.g., one or more maltodextrins having a dextrose equivalent value (DEV) of about 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25). According to some embodiments, the inoculant composition comprises one or more maltodextrins having a DEV of about 5 to about 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19 or 20, about 10 to about 11, 12, 14, 15, 16, 17, 18, 19 or 20, or about 15 to about 16, 17, 18, 19 or 20. According to some embodiments, the inoculant composition comprises a combination of maltodextrins having a DEV of about 5 to about 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19 or 20, about 10 to about 11, 12, 14, 15, 16, 17, 18, 19 or 20, or about 15 to about 16, 17, 18, 19 or 20. Non-limiting examples of maltodextrins include MALTRIN® M040 (DEV=5; molecular weight=3600; Grain Processing Corporation, Muscatine, Iowa), MALTRIN® M100 (DEV=10; molecular weight=1800; Grain Processing Corporation, Muscatine, Iowa), MALTRIN® M150 (DEV=15; molecular weight=1200; Grain Processing Corporation, Muscatine, Iowa), MALTRIN® M180 (DEV=18; molecular weight=1050; Grain Processing Corporation, Muscatine, Iowa), MALTRIN® M200 (DEV=20; molecular weight=900; Grain Processing Corporation, Muscatine, Iowa), MALTRIN® M250 (DEV=25; molecular weight=720; Grain Processing Corporation, Muscatine, Iowa); MALTRIN QD® M580 (DEV=16.5-19.9; Grain Processing Corporation, Muscatine, Iowa); MALTRIN QD® M585 (DEV=15.0-19.9; Grain Processing Corporation, Muscatine, Iowa); MALTRIN QD® M600 (DEV=20.0-23.0; Grain Processing Corporation, Muscatine, Iowa); GLOBE® Plus 15 DE (Ingredion Inc., Westchester, Ill.); and combinations thereof.
- In some embodiments, the inoculant composition comprises one or more monosaccharides (e.g., allose, altrose, arabinose, fructose, galactose, glucose, gulose, iodose, lyxose, mannose, ribose, talose, threose and/or xylose). According to some embodiments, the inoculant composition comprises gluscose. According to some embodiments, the inoculant composition does not comprise glucose.
- In some embodiments, the inoculant composition comprises one or more disaccharides (e.g., cellobiose, chitobiose, gentiobiose, gentiobiulose, isomaltose, kojibiose, lactose, lactulose, laminaribiose, maltose (e.g., maltose monohydrate, anhydrous maltose), maltulose, mannobiose, melibiose, melibiulose, nigerose, palatinose, rutinose, rutinulose, sophorose, sucrose, trehalose, turanose and/or xylobiose). According to some embodiments, the inoculant composition comprises maltose. According to some embodiments, the inoculant composition does not comprise maltose. According to some embodiments, the inoculant composition comprises trehalose. According to some embodiments, the inoculant composition does not comprise trehalose.
- In some embodiments, the inoculant composition comprises one or more oligosaccharides (e.g., fructo-oligosaccharides, galacto-oligosaccharides, mannon-oligosaccharides and/or raffinose).
- In some embodiments, the inoculant composition comprises one or more sugar alcohols (e.g., arabitol, erythritol, fucitol, galactitol, glycerol, iditol, inositol, isomalt, lactitol, maltitol, maltotetraitol, maltotriitol, mannitol, polyglycitol, ribitol, sorbitol, threitol, volemitol and/or xylitol).
- In some embodiments, the inoculant composition comprises one or more humic acids (e.g., one or more leonardite humic acids, lignite humic acids, peat humic acids and water-extracted humic acids). In some embodiments, the inoculant composition comprises ammonium humate, boron humate, potassium humate and/or sodium humate. In some embodiments, one or more of ammonium humate, boron humate, potassium humate and sodium humate is/are excluded from the inoculant composition. Nonlimiting examples of humic acids that may be useful in embodiments of the present disclosure include MDL Number MFCD00147177 (CAS Number 1415-93-6), MDL Number MFCD00135560 (CAS Number 68131-04-4), MDL Number MFCS22495372 (CAS Number 68514-28-3), CAS Number 93924-35-7, and CAS Number 308067-45-0.
- In some embodiments, the inoculant composition comprises one or more fulvic acids (e.g., one or more leonardite fulvic acids, lignite fulvic acids, peat fulvic acids and/or water-extracted fulvic acids). In some embodiments, the inoculant composition comprises ammonium fulvate, boron fulvate, potassium fulvate and/or sodium fulvate. In some embodiments, one or more of ammonium fulvate, boron fulvate, potassium fulvate and sodium fulvate is/are excluded from inoculant compositions of the present disclosure. Nonlimiting examples of fulvic acids that may be useful in embodiments of the present disclosure include MDL Number MFCD09838488 (CAS Number 479-66-3).
- In some embodiments, the inoculant composition comprises one or more betaines (e.g., trimethylglycine).
- In some embodiments, the inoculant composition comprises one or more peptones (e.g., bacterial peptones, meat peptones, milk peptones, vegetable peptones and yeast peptones).
- In some embodiments, the inoculant composition comprises one or more oxidation control components (e.g., one or more antioxidants and/or oxygen scavengers). According to some embodiments, the inoculant composition comprises one or more oxygen scavengers, such as ascrobic acid, ascorbate salts, catechol and/or sodium hydrogen carbonate. According to some embodiments, the inoculant composition comprises one or more antioxidants, such as ascorbic acid, ascorbyl palmitate, ascorbyl stearate, calcium ascorbate, carotenoids, lipoic acid, phenolic compounds (e.g., flavonoids, flavones, flavonols), potassium ascorbate, sodium ascorbate, thiols (e.g., glutathione, lipoic acid, N-acetyl cysteine), tocopherols, tocotrienols, ubiquinone and/or uric acid. Non-limiting examples of antioxidants include those that are soluble in the cell membrane (e.g., alpha tocopherol (vitamin E), ascorbyl palmitate) and those that are soluble in water (e.g., ascorbic acid and isomers or ascorbic acid, sodium or potassium salts of ascorbic acid or isomers or ascorbic acid, glutathione, sodium or potassium salts of glutathione). In some embodiments, use of a membrane-soluble antioxidant necessitates the addition of one or more surfactants to adequately disperse the antioxidant within the inoculant composition. According to some embodiments, the inoculant composition is/comprises ascorbic acid and/or glutathione.
- In some embodiments, the inoculant composition comprises one or more hygroscopic polymers (e.g., hygroscopic agars, albumins, alginates, carrageenans, celluloses, gums (e.g., cellulose gum, guar gum, gum arabic, gum combretum, xantham gum), methyl celluloses, nylons, pectins, polyacrylic acids, polycaprolactones, polycarbonates, polyethylene glycols (PEG), polyethylenimines (PEI), polylactides, polymethylacrylates (PMA), polyurethanes, polyvinyl alcohols (PVA), polyvinylpyrrolidones (PVP), propylene glycols, sodium carboxymethyl celluloses and/or starches). Non-limiting examples of polymers include AGRIMER™ polymers (e.g., 30, AL-10 LC, AL-22, AT/ATF, VA 3E, VA 31, VA 5E, VA 51, VA 6, VA 6E, VA 7E, VA 71, VEMA AN-216, VEMA AN-990, VEMA AN-1200, VEMA AN-1980, VEMA H-815MS; Ashland Specialty Ingredients, Wilmington, Del.), EASYSPERSE™ polymers (Ashland Specialty Ingredients, Wilmington, Del.); DISCO™ AG polymers (e.g., L-250, L-280, L-285, L-286, L-320, L-323, L-517, L-519, L-520, L800; Incotec Inc., Salinas, Calif.), KELZAN® polymers (Bri-Chem Supply Ltd., Calgary, Alberta, CA), TICAXAN® xanthan powders, such as PRE-HYDRATED® TICAXAN® Rapid-3 Powder (TIC Gums, White Marsh, Md.) and combinations thereof. Additional examples of polymers may be found in Pouci, et al. A
M . J. AGRIC . BIOL . SCI . 3(1):299 (2008). - In some embodiments, the inoculant composition comprises one or more UV protectants (e.g., one or more aromatic amino acids (e.g., tryptophan, tyrosine), carotenoids, cinnamates, lignosulfonates (e.g., calcium lignosulfonate, sodium lignosulfonate), melanins, mycosporines, polyphenols and/or salicylates). Non-limiting examples of UV protectants include Borregaard LignoTech™ lignosulfonates (e.g., Borresperse 3A, Borresperse CA, Borresperse NA, Marasperse AG, Norlig A, Norlig 11D, Ufoxane 3A, Ultrazine NA, Vanisperse CB; Borregaard Lignotech, Sarpsborg, Norway) and combinations thereof.
- Inoculant compositions of the present disclosure may comprise any suitable excipient(s), including, but not limited to, dispersants, drying agents, anti-freezing agents, flowability agents, safeners, anti-settling agents, pH buffers and adhesives.
- Inoculant compositions of the present disclosure may comprise any suitable dispersant(s), including, but not limited to, surfactants and wetting agents. Selection of appropriate dispersants will depend on the intended application(s) and the microorganism(s) present in the inoculant composition. In general, the dispersant(s) will have low toxicity for the microorganism(s) in the inoculant composition and for the medium(s) to which the inoculant composition is to be applied. Non-limiting examples of dispersants include Atlox™ (e.g., 4916, 4991; Croda International PLC, Edison, N.J.), Atlox METASPERSE™ (Croda International PLC, Edison, N.J.), BIO-SOFT® (e.g., N series, such as N1-3, N1-7, N1-5, N1-9, N23-3, N2.3-6.5, N25-3, N25-7, N25-9, N91-2.5, N91-6, N91-8; Stepan Company, Northfield, Ill.), MAKON® nonionic surfactants (e.g., DA-4, DA-6 and DA-9; Stepan Company, Northfield, Ill.), MORWET® powders (Akzo Nobel Surface Chemistry LLC, Chicago, Ill.), MULTIWET™ surfactants (e.g., MO-85P-PW-(AP); Croda International PLC, Edison, N.J.), SILWET® L-77 (Helena Chemical Company, Collierville, Tenn.), SPAN™ surfactants (e.g., 20, 40, 60, 65, 80 and 85; Croda Inc., Edison N.J.), TAMOL™ dispersants (The Dow Chemical Company, Midland, Mich.), TERGITOL™ surfactants (e.g., TMN-6 and TMN-100×; The Dow Chemical Company, Midland, Mich.), TERSPERSE surfactants (e.g., 2001, 2020, 2100, 2105, 2158, 2700, 4894 and 4896; Hunstman Corp., The Woodlands, Tex.), TRITON™ surfactants (e.g., X-100; The Dow Chemical Company, Midland, Mich.), TWEEN® surfactants (e.g., TWEEN® 20, 21, 22, 23, 28, 40, 60, 61, 65, 80, 81 and 85; Croda International PLC, Edison, N.J.) and combinations thereof. In some embodiments, inoculant compositions of the present disclosure comprise one or more anionic surfactants. According to some embodiments, the inoculant composition comprises one or more water-soluble anionic surfactants and/or one or more water-insoluble anionic surfactants, optionally one or more anionic surfactants selected from the group consisting of alkyl carboxylates (e.g., sodium stearate), alkyl sulfates (e.g., alkyl lauryl sulfate, sodium lauryl sulfate), alkyl ether sulfates, alkyl amido ether sulfates, alkyl aryl polyether sulfates, alkyl aryl sulfates, alkyl aryl sulfonates, alkyl sulfonates, alkyl amide sulfonates, alkyl aryl sulfonates, alkyl benzene sulfonates, alkyl diphenyloxide sulfonate, alpha-olefin sulfonates, alkyl naphthalene sulfonates, paraffin sulfonates, alkyl sulfosuccinates, alkyl ether sulfosuccinates, alkylamide sulfosuccinates, alkyl sulfosuccinamates, alkyl sulfoacetates, alkyl phosphates, alkyl ether phosphates, acyl sarconsinates, acyl isethionates, N-acyl taurates, N-acyl-N-alkyltaurates, benzene sulfonates, cumene sulfonates, dioctyl sodium sulfosuccinate, ethoxylated sulfosuccinates, lignin sulfonates, linear alkylbenzene sulfonates, monoglyceride sulfates, perfluorobutanesulfonate, perfluorooctanesulfonate, phosphate ester, styrene acrylic polymers, toluene sulfonates and xylene sulfonates.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more cationic surfactants. According to some embodiments, the inoculant composition comprises one or more pH-dependent amines and/or one or more quaternary ammonium cations, optionally one or more cationic surfactants selected from the group consisting of alkyltrimethylammonium salts (e.g., cetyl trimethylammonium bromide, cetyl trimethylammonium chloride), cetylpyridinium chloride, benzalkonium chloride, benzethonium chloride, 5-Bromo-5-nitro-1,3-dioxane, dimethyldioctadecylammonium chloride, cetrimonium bromide, dioctadecyldimethylammonium bromide and/or octenidine dihydrochloride.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more nonionic surfactants. According to some embodiments, the inoculant composition comprises one or more water-soluble nonionic surfactants and/or one or more water-insoluble nonionic surfactants, optionally one or more nonionic surfactants selected from the group consisting of alcohol ethoxylates (e.g., TERGITOL™ 15-S surfactants, such as TERGITOL™15-S-9 (The Dow Chemical Company, Midland, Mich.)), alkanolamides, alkanolamine condensates, carboxylic acid esters, cetostearyl alcohol, cetyl alcohol, cocamide DEA, dodecyldimethylamine oxides, ethanolamides, ethoxylates of glycerol ester and glycol esters, ethylene oxide polymers, ethylene oxide-propylene oxide copolymers, glucoside alkyl ethers, glycerol alkyl ethers, glycerol esters, glycol alkyl ethers (e.g., polyoxyethylene glycol alkyl ethers, polyoxypropylene glycol alkyl ethers), glycol alkylphenol ethers (e.g., polyoxyethylene glycol alkylphenol ethers), glycol esters, monolaurin, pentaethylene glycol monododecyl ethers, poloxamer, polyamines, polyglycerol polyricinoleate, polysorbate, polyoxyethylenated fatty acids, polyoxyethylenated mercaptans, polyoxyethylenated polyoxyproylene glycols, polyoxyethylene glycol sorbitan alkyl esters, polyethylene glycol-polypropylene glycol copolymers, polyoxyethylene glycol octylphenol ethers, polyvinyl pynolidones, sugar-based alkyl polyglycosides, sulfoanylamides, sorbitan fatty acid alcohol ethoxylates, sorbitan fatty acid ester ethoxylates, sorbitan fatty acid ester and/or tertiary acetylenic glycols.
- In some embodiments, inoculant compositions of the present disclosure comprise at least one nonionic surfactant. According to some embodiments, the inoculant composition comprises at least one water insoluble nonionic surfactant and at least one water soluble nonionic surfactant. In some embodiments, inoculant compositions of the present disclosure comprise a combination of nonionic surfactants having hydrocarbon chains of substantially the same length.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more zwitterionic surfactants. According to some embodiments, the inoculant composition comprises one or more betaines and/or one or more sultaines, optionally one or more zwitterionic surfactants selected from the group consisting of 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate, cocamidopropyl betaine, cocamidopropyl hydroxysultaine, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine and/or one or more sphingomyelins.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more soaps and/or organosilicone surfactants. According to some embodiments, the inoculant composition comprises one or more alkali metal salts of fatty acids.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more wetting agents. According to some embodiments, the inoculant composition comprises one or more naphthalene sulfonates, optionally one or more alkyl naphthalene sulfonates (e.g., sodium alkyl naphthalene sulfonate), one or more isopropyl naphthalene sulfonates (e.g., sodium isopropyl naphthalene sulfonate) and/or one or more butyl naphthalene sulfonates (e.g., sodium n-butyl naphthalene sulfonate).
- Inoculant compositions of the present disclosure may comprise any suitable drying agent(s), including, but not limited to, drying powders. Non-limiting examples of drying agents include AEROSIL® hydrophobic fumed silica powders (Evonik Corporation, Parsippany, N.J.), BENTOLITE® powders (BYK-Chemie GmbH, Wesel, Germany), INCOTEC® powders (INCOTEC Inc., Salinas, Calif.), SIPERNAT® silica powders (Evonik Corporation, Parsippany, N.J.) and combinations thereof. In some embodiments, inoculant compositions of the present disclosure comprise calcium stearate, clay (e.g., attapulgite clay, montmorillonite clay), graphite, magnesium stearate, magnesium sulfate, powdered milk, silica (e.g., fumed silica, hydrophobically-coated silica, precipitated silica), soy lecithin and/or talc.
- Inoculant compositions of the present disclosure may comprise any suitable anti-freezing agent(s), including, but not limited to, ethylene glycol, glycerin, propylene glycol and urea.
- Inoculant compositions of the present disclosure may comprise any flowability agent to improve the lubricity of treated materials. The flowability agent may comprise one or more liquid lubricants, solid lubricants, liquid emulsions, or suspensions of solid lubricants. Non-limiting examples of flowability agents include, for example, lubricants such as fats and oils, natural and synthetic waxes, graphite, talc, fluoropolymers (e.g., polytetrafluoroethylene), and solid lubricants such as molybdenum disulfide and tungsten disulfide. In some instances, the flowability agent comprises a wax material. Non-limiting examples of wax materials that can be incorporated into the liquid treatment composition include plant and animal-derived waxes such as carnauba wax, candelilla wax, ouricury wax, beeswax, spermaceti, and petroleum derived waxes, such as paraffin wax. For example, in some instances, the flowability agent comprises carnauba wax. In some instances, the flowability agent comprises an oil. For example, the flowability agent may comprise soybean oil. Non-limiting examples of commercially available wax materials suitable for use as flowability agents include AQUAKLEAN 418 supplied by Micro Powders, Inc. (an anionic aqueous emulsion comprising extra light carnauba wax at 35% solids content).
- Inoculant compositions of the present disclosure may comprise any suitable safener(s), including, but not limited to, napthalic anhydride.
- Inoculant compositions of the present disclosure may comprise any suitable pH buffer(s), including, but not limited to, potassium phosphate monobasic and potassium phosphate dibasic. In some embodiments, the inoculant composition comprises one or more pH buffers selected to provide a composition having a pH of less than 10, typically from about 4.5 to about 9.5, from about 6 to about 8, or about 7.
- Inoculant compositions of the present disclosure may comprise any suitable anti-settling agent(s), including, but not limited to, polyvinyl acetate, polyvinyl alcohols with different degrees of hydrolysis, polyvinylpyrrolidones, polyacrylates, acrylate-, polyol- or polyester-based paint system binders which are soluble or dispersible in water, moreover copolymers of two or more monomers such as acrylic acid, methacrylic acid, itaconic acid, maleic acid, fumaric acid, maleic anhydride, vinylpyrrolidone, ethylenically unsaturated monomers such as ethylene, butadiene, isoprene, chloroprene, styrene, divinylbenzene, ot-methylstyrene or p-methylstyrene, further vinyl halides such as vinyl chloride and vinylidene chloride, additionally vinyl esters such as vinyl acetate, vinyl propionate or vinyl stearate, moreover vinyl methyl ketone or esters of acrylic acid or methacrylic acid with monohydric alcohols or polyols such as methyl acrylate, methyl methacrylate, ethyl acrylate, ethylene methacrylate, lauryl acrylate, lauryl methacrylate, decyl acrylate, N,N-dimethylamino-ethyl methacrylate, 2-hydroxyethyl methacrylate, 2-hydroxypropyl methacrylate or glycidyl methacrylate, furthermore diethyl esters or monoesters of unsaturated dicarboxylic acids, furthermore (meth)acrylamido-N-methylol methyl ether, amides or nitriles such as acrylamide, methacrylamide, N-methylol(meth)acrylamide, acrylonitrile, methacrylonitrile, and also N-substituted maleiraides and ethers such as vinyl butyl ether, vinyl isobutyl ether or vinyl phenyl ether, and combinations thereof.
- Inoculant compositions of the present disclosure may comprise any suitable adhesive(s), including, but not limited to, adhesive compositions comprising, consisting essentially of or consisting of one or more disaccharides (e.g. maltose), gums (e.g., cellulose gum, guar gum, gum arabic, gum combretum, xantham gum), maltodextrins (e.g., one or more maltodextrins (each and/or collectively) having a DEV of about 10 to about 20), monosaccharides, oils (e.g., mineral oil, olive oil, peanut oil, soybean oil and/or sunflower oil) and/or oligosaccharides.
- Inoculant compositions of the present disclosure may comprise any suitable effect pigment(s). Effect pigments, which are sometimes also referred to in the art as “pearl pigments,” are a class of materials that provide reflectivity, shine, and/or a pearlescent effect when applied as a coating. In some instances, the effect pigment is in the form of a powder comprising a substrate material and a metal oxide coating. For example, the effect pigment may comprise a substrate material including but not limited to talc, silicate materials (e.g., mica), clay minerals, calcium carbonate, kaolin, phlogopite, alumina, and similar substances. In some instances, the substrate material comprises a hydrophilic material. The substrate material may be coated with a semi-transparent layer of a metal oxide, including but not limited to titanium dioxide, iron oxide, chromium oxide, or zirconium oxide. Alternatively, in some instances, the effect pigment comprises metal powder or metal flakes. The metal powder or metal flakes may comprise a metal including, but not limited to aluminum, copper, silver, or bronze. In some instances, the effect pigment comprises a silicate based substrate. Non-limiting examples of particulate silicates that can be incorporated into the dry powder coating include mica coated with titanium dioxide (e.g., SUNMICA FINE WHITE 2800102, which is commercially available from Sun Chemical Corp.). Other non-limiting examples of commercially available effect pigments that can be incorporated into the dry powder include MAGNA PEARL, LUMINA and MEARLIN pigments from BASF Corporation; PHIBRO PEARL from PhibroChem; and IRIDESIUM 120 from Aakash Chemicals. In some instances, the dry powder has a mean particle size of from about 1 to about 25 microns.
- Inoculant compositions of the present disclosure may comprise any growth medium suitable for culturing one or more of the microorganisms in the inoculant composition. For example, in some embodiments, inoculant compositions of the present disclosure comprise Czapek-Dox medium, glycerol yeast extract, mannitol yeast extract, potato dextrose broth and/or YEM media.
- Carriers, stabilizing compounds, dispersants, drying agents, safeners, flowability agents, anti-settling agents, buffers, adhesives, etc. may be incorporated into inoculant compositions of the present disclosure in any suitable amount(s)/concentration(s). The absolute value of the amount/concentration that is/are sufficient to cause the desired effect(s) may be affected by factors such as the type, size and volume of material to which the compositon will be applied, the type(s) of microorganisms in the composition, the number of microorganisms in the composition, the stability of the microorganisms in the composition and storage conditions (e.g., temperature, relative humidity, duration). Those skilled in the art will understand how to select effective amounts/concentrations using routine dose-response experiments. Guidance for the selection of appropriate amounts/concentrations can be found, for example, in International Patent Application Nos. PCT/US2016/050529 and PCT/US2016/050647 and U.S. Provisional Patent Application Nos. 62/296,798; 62/271,857; 62/347,773; 62/343,217; 62/296,784; 62/271,873; 62/347,785; 62/347,794; and 62/347,805.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more carriers in an amount/concentration of about 1 to about 99% or more (by weight, based upon the total weight of the inoculant composition). For example, inoculant compositions of the present disclosure may comprsise about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98 or 99% (by weight) of one or more non-aqueous carriers.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more stabilizing compounds in an amount/concentration of about 0.0001 to about 95% or more (by weight, based upon the total of the inoculant composition). For example, inoculant compositions of the present disclosure may comprise about 0.0001 to about 0.001, about 0.001 to about 1%, about 0.25 to about 5%, about 1 to about 10%, about 5 to about 25%, about 10% to about 30%, about 20% to about 40%, about 25% to about 50%, about 30 to about 60%, about 50 to about 75%, or about 75 to about 95% (by weight), optionally about 0.0005, 0.001, 0.002, 0.003, 0.004, 0.005, 0.0075, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95%, of one or more maltodextrins, monosaccharides, disaccharides, sugar alcohols, humic acids, betaines, prolines, sarcosines, peptones, oxidation control components, hygroscopic polymers and/or UV protectants.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more stabilizing compounds at a concentration of about 1×10−20 M to about 1×10−1 M. For example, inoculant compositions of the present disclosure may comprise about 1×10−15 M to about 1×10−10 M, about 1×10−14 M to about 1×10−8 M, about 1×10−14 M to about 1×10−6 M, about 1×10−12 M to about 1×10−8 M, about 1×10−12 M to about 1×10−6 M, about 1×10−10 M to about 1×10−6 M, or about 1×10−8 M to about 1×10−2 M, optionally about 1×10−20 M, 1×10−19 M, 1×10−18 M, 1×10−17 M, 1×10−16 M, 1×10−15 M, 1×10−14 M, 1×10−13 M, 1×10−12 M, 1×10−11 M, 1×10−1 M, 1×10−9 M, 1×10−8 M, 1×10−7 M, 1×10−6 M, 1×10−5 M, 1×10−4 M, 1×10−3 M, 1×10−2 M, 1×10−1 M or more, ofone or more maltodextrins, monosaccharides, disaccharides, sugar alcohols, humic acids, betaines, prolines, sarcosines, peptones, oxidation control components, hygroscopic polymers and/or UV protectants.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more monosaccharides in an amount/concentration of about 0.005 to about 50% (by weight) of the inoculant composition. For example, inoculant compositions of the present disclosure may comprise about/at least/less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.75, 1, 1.25, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 10, 15, 20, 25% (by weight) of one or more monosaccharides (e.g., arabinose, fructose and/or glucose). In some embodiments, one or more monosaccharides is/are present in a concentration ranging from about 1×10−20 M to about 1×10−1 M. For example, one or more monosaccharides may be included at a concentration of about/at least/less than 1×10−20 M, 1×10−19 M, 1×10−18 M, 1×10−17 M, 1×10−16 M, 1×10−15 M, 1×10-14 M, 1×10−13 M, 1×10−12 M, 1×10−11 M, 1×10−1° M.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more disaccharides in an amount/concentration of about 0.005 to about 50% (by weight) of the inoculant composition. For example, inoculant compositions of the present disclosure may comprise about/at least/less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.75, 1, 1.25, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 10, 15, 20, 25% (by weight) of one or more disaccharides (e.g., maltose, sucrose and/or trehalose). In some embodiments, one or more disaccharides is/are present in a concentration ranging from about 1×10-20 M to about 1×10−1 M. For example, one or more disaccharides may be included at a concentration of about/at least/less than 1×10−20 M, 1×10−19 M, 1×10−18 M, 1×10−17 M, 1×10−16 M, 1×10−15 M, 1×10−14 M, 1×10−13 M, 1×10−12 M, 1×10−11 M, 1×10−10 M.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more maltodextrins in an amount/concentration of about 0.001 to about 95% or more (by weight) of the inoculant composition. In some embodiments, the maltodextrin(s) comprise(s) about 0.001 to about 1%, about 0.25 to about 5%, about 1 to about 10%, about 5 to about 25%, about 10% to about 30%, about 20% to about 40%, about 25% to about 50%, about 50 to about 75%, or about 75 to about 95% (by weight) of the inoculant composition. For example, inoculant compositions of the present disclosure may comprise about/at least/less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of one or more maltodextrins (e.g., one or more maltodextrins (each and/or collectively) having a DEV value of about 15 to about 20).
- In some embodiments, inoculant compositions of the present disclosure comprise one or more sugar alcohols in an amount/concentration of about 0.001 to about 95% or more (by weight) of the inoculant composition. In some embodiments, the sugar alcohol(s) (e.g., arabitol, mannitol, sorbitol and/or xylitol) comprise(s) about 0.001 to about 1%, about 0.25 to about 5%, about 1 to about 10%, about 5 to about 25%, about 10% to about 30%, about 20% to about 40%, about 25% to about 50%, about 50 to about 75%, or about 75 to about 95% (by weight) of the inoculant composition. For example, inoculant compositions of the present disclosure may comprise about/at least/less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of one or more sugar alcohols (e.g., arabitol, mannitol, sorbitol and/or xylitol).
- In some embodiments, inoculant compositions of the present disclosure comprise one or more humic acids in an amount/concentration of about 0.001 to about 95% or more (by weight) of the inoculant composition. In some embodiments, the humic acid(s) (e.g., potassium humate) comprise(s) about 0.001 to about 1%, about 0.25 to about 5%, about 1 to about 10%, about 5 to about 25%, about 10% to about 30%, about 20% to about 40%, about 25% to about 50%, about 50 to about 75%, or about 75 to about 95% (by weight) of the inoculant composition. For example, inoculant compositions of the present disclosure may comprise about/at least/less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of one or more humic acids (e.g., potassium humate and/or sodium humate).
- In some embodiments, inoculant compositions of the present disclosure comprise one or more UV protectants in an amount/concentration of about 0.0001 to about 5% or more (by weight) of the inoculant composition. In some embodiments, the UV protectant(s) (e.g., calcium lignosulfate and/or sodium lignosulfate) comprise(s) about 0.0001 to about 0.001, about 0.001 to about 1%, about 0.25 to about 5%, (by weight) of the inoculant composition. For example, inoculant compositions of the present disclosure may comprise about/at least/less than 0.0005, 0.001, 0.002, 0.003, 0.004, 0.005, 0.0075, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.75, 1, 1.25, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5% or more (by weight) of one or more UV protectants (e.g., calcium lignosulfate and/or sodium lignosulfate).
- In some embodiments, inoculant compositions of the present disclosure comprise one or more oxidation control components in an amount/concentration of about 0.0001 to about 5% or more (by weight) of the composition. For example, inoculant compositions of the present disclosure may comprise about/at least/less than 0.0005, 0.001, 0.002, 0.003, 0.004, 0.005, 0.0075, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.75, 1, 1.25, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5% of one or more oxidation control components. In some embodiments, the amount/concentration of oxidation control components is about 0.005 to about 2% (by weight) of the composition. In some embodiments, the oxidation control component(s) is/are present in a concentration ranging from about 1×10−20 M to about 1×10−1 M. For example, one or more oxidation control components may be added at a concentration of about/at least/less than 1×10−20 M, 1×10−19 M, 1×10−18 M, 1×10−17 M, 1×10−16 M, 1×10−15 M, 1×10−14 M, 1×10−13 M, 1×10−12 M, 1×1011 M, 1×1010 M. In some embodiments, inoculant compositions of the present disclosure comprise one or more commercial antioxidants used in accordance with the manufacturer's recommended amounts/concentrations. In some embodiments, inoculant compositions of the present disclosure comprise one or more commercial oxygen scavengers used in accordance with the manufacturer's recommended amounts/concentrations.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more stabilizing compounds in an amount/concentration sufficient to ensure the fungal cells and/or spores remain viable following:
- storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more;
- desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- cryopreservation at or below −80° C. for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- application;
- application and desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; and/or
- application and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more stabilizing compounds in an amount/concentration sufficient to ensure at least 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% of the fungal cells and/or spores remain viable following:
- storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more;
- desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- cryopreservation at or below −80° C. for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- application;
- application and desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; and/or
- application and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more stabilizing compounds in an amount/concentration sufficient to ensure at least 1×101, 1×102, 1×103, 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010 or more colony-forming units of the fungal cells and/or spores remain viable per gram and/or milliliter of inoculant composition following:
- storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more;
- desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; cryopreservation at or below −80° C. for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- application;
- application and desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; and/or
- application and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more stabilizing compounds in an amount/concentration sufficient to ensure the deliquescence relative humidity (DRH) of the inoculant composition is less than 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85 or 90 at the temperature(s) at which the composition is to be stored (e.g., 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C.).
- In some embodiments, inoculant compositions of the present disclosure comprise two or more stabilizing compounds that synergistically enhance the stability and/or survival of the fungal cells and/or spores.
- Stabilizing compounds may be incorporated into inoculant compositions of the present disclosure in any suitable ratio(s).
- In some embodiments, inoculant compositions of the present disclosure comprise one or more maltodextrins and one or more monosaccharides, disaccharides, sugar alcohols and/or humic acids in a maltodextrin:(monosaccharide, disaccharide, sugar alcohol and/or humic acid) ratio of about 5:95, 10:90, 15:85, 20:80, 25:75, 30:70, 35:65, 40:60, 45:55, 50:50, 55:45, 60:40, 65:35, 70:30, 75:25, 80:20, 85:15, 90:10, 95:5. For example, inoculant compositions of the present disclosure may comprise one or more maltodextrins (e.g., one or more maltodextrins (each and/or collectively) having a DEV of about 15 to about 20) and one or more sugar alcohols (e.g., sorbitol and/or xylitol) and/or humic acids (e.g., potassium humate) in a maltodextrin:(sugar alcohol/humic acid) ratio of about 5:95, about 15:85, about 25:75 or about 50:50.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more dispersants in an amount/concentration of about 0.001 to about 25% or more (by weight) of the inoculant composition. In some embodiments, the dispersant(s) comprise(s) 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.004, 0.0045, 0.005, 0.0055, 0.006, 0.0065, 0.007, 0.0075, 0.008, 0.0085, 0.009, 0.0095, 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.02, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 6, 7, 8, 9 or 10 to about 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20% (by weight) of the inoculant composition. For example, inoculant compositions of the present disclosure may comprise about 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20% or more (by weight) of one or more dispersants (e.g., one or more surfactants and/or wetting agents).
- In some embodiments, inoculant compositions of the present disclosure comprise one or more drying agents in an amount/concentration of about 0.001 to about 95% or more (by weight) of the inoculant composition. In some embodiments, the drying agent(s) comprise(s) about) 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.004, 0.0045, 0.005, 0.0055, 0.006, 0.0065, 0.007, 0.0075, 0.008, 0.0085, 0.009, 0.0095, 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.02, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 6, 7, 8, 9 or 10 to about 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20% (by weight) of the inoculant composition. For example, inoculant compositions of the present disclosure may comprise about 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of one or more drying agents (e.g., lecithin and/or talc).
- In some embodiments, the inoculant compositions of the present disclosure comprise about 0.5 to about 10 grams of drying powder per liter of inoculant composition. For example, inoculant compositions of the present disclosure may comprise about 0.5, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10 grams or more of drying powder per liter of inoculant composition.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more buffers in an amount/concentration of about 0.0001 to about 5% or more (by weight) of the inoculant composition. In some embodiments, the buffer(s) comprise(s) about 0.0001, 0.0002, 0.0003, 0.0004, 0.0005, 0.0006, 0.0007, 0.0008, 0.0009, 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.004, 0.0045, 0.005, 0.0055, 0.006, 0.0065, 0.007, 0.0075, 0.008, 0.0085, 0.009, 0.0095, 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.02, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1 to about 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5% (by weight) of the inoculant composition. For example, inoculant compositions of the present disclosure may comprise about 0.0005, 0.00075, 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5% or more (by weight) of one or more buffers (e.g., potassium phosphate monobasic and/or potassium phosphate dibasic).
- In some embodiments, inoculant compositions of the present disclosure comprise one or more commercial carriers, antioxidants, oxygen scavengers, hygroscopic polymers, UV protectants, disperants, drying agents, anti-freezing agents, buffers and/or adhesives used in accordance with the manufacturer's recommended amounts/concentrations.
- In some embodiments, the fungal strain(s) of the present disclosure is/are the only microbial strain(s) in inoculant compositions of the present disclosure.
- In some embodiments, inoculant compositions of the present disclosure comprise one or more microorganisms in addition to the fungal strain(s) of the present disclosure. Any suitable microorganism(s) may be added. Selection of additional microbes (if any) will depend on the intended application(s).
- Non-limiting examples of bacteria that may be included in inoculant compositions of the present disclosure include Azospirillum brasilense INTA Az-39, Bacillus amyloliquefaciens D747, Bacillus amyloliquefaciens NRRL B 50349, Bacillus amyloliquefaciens TJ1000, Bacillus amyloliquefaciens FZB24, Bacillus amyloliquefaciens FZB42, Bacillus amyloliquefaciens IN937a, Bacillus amyloliquefaciens IT-45, Bacillus amyloliquefaciens TJ1000, Bacillus amyloliquefaciens MBI600, Bacillus amyloliquefaciens BS27 (deposited as NRRL B-5015), Bacillus amyloliquefaciens BS2084 (deposited as NRRL B-50013), Bacillus amyloliquefaciens 15AP4 (deposited as ATCC PTA-6507), Bacillus amyloliquefaciens 3AP4 (deposited as ATCC PTA-6506), Bacillus amyloliquefaciens LSSA01 (deposited as NRRL B-50104), Bacillus amyloliquefaciens ABP278 (deposited as NRRL B-50634), Bacillus amyloliquefaciens 1013 (deposited as NRRL B-50509), Bacillus amyloliquefaciens 918 (deposited as NRRL B-50508), Bacillus amyloliquefaciens 22CP1 (deposited as ATCC PTA-6508) and Bacillus amyloliquefaciens BS18 (deposited as NRRL B-50633), Bacillus cereus 1-1562, Bacillus firmus 1-1582, Bacillus lichenformis BA842 (deposited as NRRL B-50516), Bacillus lichenformis BL21 (deposited as NRRL B-50134), Bacillus mycoides NRRL B-21664, Bacillus pumilus NRRL B 21662, Bacillus pumilus NRRL B-30087, Bacillus pumilus ATCC 55608, Bacillus pumilus ATCC 55609, Bacillus pumilus GB34, Bacillus pumilus KFP9F, Bacillus pumilus QST 2808, Bacillus subtilis ATCC 55078, Bacillus subtilis ATCC 55079, Bacillus subtilis MBI 600, Bacillus subtilis NRRL B-21661, Bacillus subtilis NRRL B-21665, Bacillus subtilis CX-9060, Bacillus subtilis GB03, Bacillus subtilis GB07, Bacillus subtilis QST-713, Bacillus subtilis FZB24, Bacillus subtilis D747, Bacillus subtilis 3BP5 (deposited as NRRL B-50510), Bacillus thuringiensis ATCC 13367, Bacillus thuringiensis GC-91, Bacillus thuringiensis NRRL B-21619, Bacillus thuringiensis ABTS-1857, Bacillus thuringiensis SAN 401 I, Bacillus thuringiensis ABG-6305, Bacillus thuringiensis ABG-6346, Bacillus thuringiensis AM65-52, Bacillus thuringiensis SA-12, Bacillus thuringiensis SB4, Bacillus thuringiensis ABTS-351, Bacillus thuringiensis HD-1, Bacillus thuringiensis EG 2348, Bacillus thuringiensis EG 7826, Bacillus thuringiensis EG 7841, Bacillus thuringiensis DSM 2803, Bacillus thuringiensis NB-125, Bacillus thuringiensis NB-176, BRADY, Pseudomonas jessenii PS06, Rhizobium leguminosarum SO12A-2 (IDAC 080305-01), Sinorhizobium fredii CCBAU114, Sinorhizobium fredii USDA 205, Yersinia entomophaga 082KB8 and combinations thereof, as well as microorganisms having at least at least 75, 80, 85, 90, 95, 96, 97, 97.5, 98, 98.5, 99, 99.5, 99.6, 99.7, 99.8, 99.9% or more identical to any of the aforementioned strains on the basis of 16S rDNA sequence identity.
- Non-limiting examples of fungi that may be included in inoculant compositions of the present disclosure include Gliocladium virens ATCC 52045, Gliocladium virens GL-21, Glomus intraradices RTI-801, Metarhizium anisopliae F52, PENI, Trichoderma asperellum SKT-1, Trichoderma asperellum ICC 012, Trichoderma atroviride LC52, Trichoderma atroviride CNCM 1-1237, Trichoderma fertile JM41R, Trichoderma gamsii ICC 080, Trichoderma hamatum ATCC 52198, Trichoderma harzianum ATCC 52445, Trichoderma harzianum KRL-AG2, Trichoderma harzianum T-22, Trichoderma harzianum TH-35, Trichoderma harzianum T-39, Trichoderma harzianum ICC012, Trichoderma reesi ATCC 28217, Trichoderma virens ATCC 58678, Trichoderma virens Gl-3, Trichoderma virens GL-21, Trichoderma virens G-41, Trichoderma viridae ATCC 52440, Trichoderma viridae ICC080, Trichoderma viridae TV1 and combinations thereof, as well as microorganisms having at least at least 75, 80, 85, 90, 95, 96, 97, 97.5, 98, 98.5, 99, 99.5, 99.6, 99.7, 99.8, 99.9% or more identical to any of the aforementioned strains on the basis of internal transcribed spacer (ITS) and/or cytochrome c oxidase (CO1) sequence identity.
- Non-limiting examples of mycorrhizal fungi that may be included in inoculant compositions of the present disclosure include mycorrhizal strains such as Gigaspora margarita, Glomus aggregatum, Glomus brasilianum, Glomus clarum, Glomus deserticola, Glomus etunicatum, Glomus intraradices, Glomus monosporum, Glomus mosseae, Laccaria bicolor, Laccaria laccata, Paraglomus brazilianum, Pisolithus tinctorius, Rhizopogon amylopogon, Rhizopogon fulvigleba, Rhizopogon luteolus, Rhizopogon villosuli, Scleroderma cepa and Scleroderma citrinum and combinations thereof.
- Additional examples of microorganisms that may be added to inoculant compositions of the present disclosure can be found in Appendix A.
- Additional microorganisms may be incorporated into inoculant compositions of the present disclosure in any suitable amount(s)/concentration(s). The absolute value of the amount/concentration that is/are sufficient to cause the desired effect(s) may be affected by factors such as the type, size and volume of material to which the composition will be applied, the microorganisms in the composition, the number of microorganisms in the composition, the stability of the microorganisms in the composition and storage conditions (e.g., temperature, relative humidity, duration). Those skilled in the art will understand how to select an effective amount/concentration using routine dose-response experiments. Guidance for the selection of appropriate amounts/concentrations can be found, for example, in International Patent Application Nos. PCT/US2016/050529 and PCT/US2016/050647 and U.S. Provisional Patent Application Nos. 62/296,798; 62/271,857; 62/347,773; 62/343,217; 62/296,784; 62/271,873; 62/347,785; 62/347,794; and 62/347,805.
- In some embodiments, one or more additional microorganisms is/are present in an amount ranging from about 1×101 to about 1×1012 colony-forming units (cfu) per gram and/or milliliter of inoculant composition. According to some embodiments, the inoculant composition comprises about 1×101, 1×102, 1×103, 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010, 1×1011, 1×1012 or more cfu of one or more additional microorganisms per gram and/or milliliter of inoculant composition (e.g., about 1×104 to about 1×109 cfu/g of Bacillus amyloliquefaciens TJ1000 (also known as 1BE, isolate ATCC BAA-390), BRADY, Metarhizium anisopliae F52, PENI, Trichoderma virens Gl-3, and/or Yersinia entomophaga 082KB8). In some embodiments, inoculant compositions of the present disclosure comprise at least 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010, 1×1011, 1×1012 cfu of one or more additional microorganisms per gram and/or millilitre of inoculant composition.
- In some embodiments, spores from one or more additional microorganims comprise about 0.1 to about 90% (by weight) of the inoculant composition. According to some embodiments, the inoculant composition comprises about 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) of microbial spores from one or more additional microorganisms (e.g., about 10% Bacillus amyloliquefaciens TJ1000, Metarhizium anisopliae F52, Penicillium bilaiae ATCC 20851, Penicillium bilaiae RS7B-SD1 and/or Trichoderma virens Gl-3 spores). In some embodiments, the amount/concentration of microbial spores from one or more additional microorganisms is about 1 to about 25%, about 5 to about 20%, about 5 to about 15%, about 5 to about 10% or about 8 to about 12% (by weight) of the inoculant composition.
- It is to be understood that additional microorganisms in inoculant compositions of the present disclosure may comprise vegetative cells and/or dormant spores. According to some embodiments, at least 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98, 99% or more additional microorganims are present in inoculant compostions of the present disclosure as vegetative cells. According to some embodiments, at least 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98, 99% or more additional microorganims are present in inoculant compostions of the present disclosure as spores.
- Inoculant compositions of the present disclosure may be formulated as any suitable type of composition.
- In some embodiments, inoculant compositions of the present disclosure are formulated as amorphous solids.
- In some embodiments, inoculant compositions of the present disclosure are formulated as amorphous liquids.
- In some embodiments, inoculant compositions of the present disclosure are formulated as wettable powders.
- In some embodiments, inoculant compositions of the present disclosure are formulated as liquid compositions that are subsequently dried to produce a powder or granuale. For example, in some embodiments, liquid inoculant compositions of the present disclosure are drum dried, evaporation dried, fluidized bed dried, freeze dried, spray dried, spray-freeze dried, tray dried and/or vacuum dried to produce powders/granuales. Such powders/granuales may be further processed using any suitable method(s), including, but not limited to, flocculation, granulation and milling, to achieve a desired particle size or physical format. The precise method(s) and parameters of processing dried powders/granuales that are appropriate in a given situation may be affected by factors such as the desired particle size(s), the type, size and volume of material to which the compositon will be applied, the type(s) of microorganisms in the composition, the number of microorganisms in the composition, the stability of the microorganisms in the composition and the storage conditions (e.g., temperature, relative humidity, duration). Those skilled in the art will understand how to select appropriate methods and parameters using routine experiments.
- In some embodiments, inoculant compositions of the present disclosure are frozen for cryopreservation. For example, in some embodiments, liquid inoculant compositions of the present disclosure are flash-frozen and stored in a cryopreservation storage unit/facility. The precise method(s) and parameters of freezing and preserving inoculant compositions of the present disclosure that are appropriate in a given situation may be affected by factors such as the type(s) of microorganisms in the composition, the number of microorganisms in the composition, the stability of the microorganisms in the composition and the storage conditions (e.g., temperature, relative humidity, duration). Those skilled in the art will understand how to select appropriate methods and parameters using routine experiments.
- Inoculant compositions of the present disclosure may be formulated as aqueous or non-aqueous compositions. In some embodiments, inoculant compositions of the present disclosure comprise no water. In some embodiments, inoculant compositions of the present disclosure comprise a trace amount of water. In some embodiments, inoculant compositions of the present disclosure comprise less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75 or 5% water by weight, based upon the total weight of the composition.
- In some embodiments, inoculant compositions of the present disclosure are formulated to have a pH of about 4.5 to about 9.5. In some embodiments, inoculant compositions of the present disclosure have a pH of about 6 to about 7.5. In some embodiments, inoculant compositions of the present disclosure have a pH of about 5, 5.5, 6, 6.5, 7, 7.5, 8 or 8.5.
- As noted above, inoculant compositions of the present disclosure may contain a variety of carriers, stabilizers, dispersants, etc. It is to be understood that the components to be included in the inoculant composition and the order in which components are incorporated into the inoculant composition may be chosen or designed to maintain or enhance the dispersion, stability and/or survival of fungal cells/spores of the present disclosure during storage, distribution, and/or application of the inoculant composition.
- It is to be understood that inoculant compositions of the present disclosure are non-naturally occurring compositions. According to some embodiments, the inoculant composition comprises one or more non-naturally occurring components. According to some embodiments, the inoculant composition comprises a non-naturally occurring combination of naturally occurring components.
- The present disclosure extends to kits comprising, consisting essentially of, or consisting of two or more containers, each comprising one or more components of an inoculant composition of the present disclosure. For example, spores of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 and the carrier may be housed in separate containers for long-term storage, then combined prior to introducing/applying the inoculant composition (in)to the desired target. Optional constituents, such as stabilizing compounds, may be added to either of the two containers or housed in one or more separate containers for long-term storage. In some embodiments, the kit further comprises one or more oxygen scavengers, such as activated carbon, ascorbic acid, iron powder, mixtures of ferrous carbonate and metal halide catalysts, sodium chloride and/or sodium hydrogen carbonate.
- The containers may comprise any suitable material(s), including, but not limited to, materials that reduce the amount of light, moisture and/or oxygen that contact the inoculant composition when the container is sealed. In some embodiments, the containers comprise, consist essentially of, or consist of a material having light permeability of less than about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70 or 75%. In some embodiments, the containers comprise, consist essentially of, or consist of a material having an oxygen transmission rate of less than about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, or 500 cm3/m2·day (as measured in accordance with ASTM D3985).
- In some embodiments, the containers reduce the amount of ambient light that reaches said inoculant composition by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% when sealed.
- In some embodiments, the containers reduce the amount of ambient moisture that reaches said inoculant composition by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% when sealed.
- In some embodiments, the containers reduce the amount of ambient oxygen that reaches said inoculant composition by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% when sealed.
- The present disclosure extends to methods and uses for fungal strains and inoculant compositions of the present disclosure.
- In some embodiments, methods and uses of the present disclosure comprise, consist essentially of or consist of applying one or more fungal strains (or inoculant compositions) of the present disclosure to a medium comprising one or more insoluble forms of phosphorous (e.g., insoluble phosphates) in an amount/concentration effective to increase the amount/concentration of soluble phosphate in said medium. According to some embodiments, the fungal strain(s)/inoculant composition(s) is/are applied in an amount/concentration effective to increase the amount/concentration of soluble phosphate by at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200% or more as compared to a control medium (e.g., an identical medium treated with a control composition that is identical to the inoculant composition of the present disclosure except that it lacks one or more of the fungal strains of the present disclosure found in the inoculant composition of the present disclosure).
- In some embodiments, methods and uses of the present disclosure comprise, consist essentially of or consist of introducing one or more fungal strains (or inoculant compositions) of the present disclosure into a medium comprising one or more insoluble forms of phosphorous (e.g., insoluble phosphates) in an amount/concentration effective to increase the amount/concentration of soluble phosphate in said medium. According to some embodiments, the fungal strain(s)/inoculant composition(s) is/are introduced in an amount/concentration effective to increase the amount/concentration of soluble phosphate by at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200% or more as compared to a control medium (e.g., an identical medium treated with a control composition that is identical to the inoculant composition of the present disclosure except that it lacks one or more of the fungal strains of the present disclosure found in the inoculant composition of the present disclosure).
- In some embodiments, methods and uses of the present disclosure comprise, consist essentially of or consist of applying one or more fungal strains (or inoculant compositions) of the present disclosure to a medium comprising one or more insoluble forms of phosphorous (e.g., insoluble phosphates) in an amount/concentration effective to decrease the amount/concentration of phosphorous that must be added to the plant growth medium in order to achieve a desired outcome. According to some embodiments, the fungal strain(s)/inoculant composition(s) is/are applied in an amount/concentration effective to decrease the amount/concentration of phosphorous that must be added to the plant growth medium in order to achieve a desired outcome by at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200% or more as compared to a control medium (e.g., an identical medium treated with a control composition that is identical to the inoculant composition of the present disclosure except that it lacks one or more of the fungal strains of the present disclosure found in the inoculant composition of the present disclosure).
- In some embodiments, methods and uses of the present disclosure comprise, consist essentially of or consist of introducing one or more fungal strains (or inoculant compositions) of the present disclosure into a medium comprising one or more insoluble forms of phosphorous (e.g., insoluble phosphates) in an amount/concentration effective to decrease the amount/concentration of phosphorous that must be added to the plant growth medium in order to achieve a desired outcome. According to some embodiments, the fungal strain(s)/inoculant composition(s) is/are introduced in an amount/concentration effective to decrease the amount/concentration of phosphorous that must be added to the plant growth medium in order to achieve a desired outcome by at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200% or more as compared to a control medium (e.g., an identical medium treated with a control composition that is identical to the inoculant composition of the present disclosure except that it lacks one or more of the fungal strains of the present disclosure found in the inoculant composition of the present disclosure).
- Those skilled in the art will understand how to select an effective method of application and amount/concentration using routine dose-response experiments. In some embodiments, the fungal cells/spores are applied/introduced (in)to the medium at a rate of about 1×101 to about 1×1020 cfu per kilogram of media, optionally about/at least 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010, 1×1011, 1×1012, 1×1013, 1×1014, 1×1015 fungal cells/spores per kilogram of media. According to some embodiments, an average of about/at least 1×103, 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010, 1×1011, 1×1012 cfu of P. bilaiae NRRL B-67154, P. bilaiae NRRL B-67155, P. bilaiae NRRL B-67156, P. bilaiae NRRL B-67157, P. bilaiae NRRL B-67158, and/or P. bilaiae NRRL B-67159 are applied/introduced (in)to each kilogram of media.
- Particular embodiments of the present disclosure are described in the following numbered paragraphs:
- 1. The isolated strain of Penicillium bilaiae having the deposit accession number NRRL 67154 (P. bilaiae NRRL B-67154).
- 2. The isolated strain of Penicillium bilaiae having the deposit accession number NRRL 67155 (P. bilaiae NRRL B-67155).
- 3. The isolated strain of Penicillium bilaiae having the deposit accession number NRRL 67156 (P. bilaiae NRRL B-67156).
- 4. The isolated strain of Penicillium bilaiae having the deposit accession number NRRL 67157 (P. bilaiae NRRL B-67157).
- 5. The isolated strain of Penicillium bilaiae having the deposit accession number NRRL 67158 (P. bilaiae NRRL B-67158).
- 6. The isolated strain of Penicillium bilaiae having the deposit accession number NRRL 67159 (P. bilaiae NRRL B-67159).
- 7. The progeny of any one or more of the isolated strains of paragraphs 1-6.
- 8. A modified microbial strain derived from the isolated strain of any one of paragraphs 1-6 or the progeny of paragraph 7.
- 9. A biologically pure culture of the isolated strain of any one of paragraphs 1-6.
- 10. A biologically pure culture of the progeny of paragraph 7.
- 11. A biologically pure culture of the modified microbial strain of paragraph 8.
- 12. An inoculant composition comprising, consisting essentially of or consisting of a carrier and one or more fungal strains, said one or more fungal strains comprising, consisting essentially of or consisting of:
- the isolated strain of any one paragraphs 1-6;
- the progeny of paragraph 7; and/or
- the modified microbial strain of paragraph 8.
- 13. The inoculant composition of paragraph 12, wherein said one or more fungal strains is present in said inoculant composition in an amount/concentration ranging from about 1×101 to about 1×1015 colony-forming units of said one or more fungal strains per gram and/or milliliter of said inoculant composition, optionally 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010, 1×1011, 1×1012 or more colony-forming units per gram and/or milliliter of said inoculant composition.
- 14. The inoculant composition of paragraph 12, wherein said one or more fungal strains comprises:
- about 1×101 to about 1×1012 colony-forming units P. bilaiae NRRL B-67154 per gram and/or milliliter of said inoculant composition, optionally 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010 or more colony-forming units per gram and/or milliliter of said inoculant composition;
- about 1×101 to about 1×1012 colony-forming units of P. bilaiae NRRL B-67155 per gram and/or milliliter of said inoculant composition, optionally 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010 or more colony-forming units per gram and/or milliliter of said inoculant composition;
- about 1×101 to about 1×1012 colony-forming units of P. bilaiae NRRL B-67156 per gram and/or milliliter of said inoculant composition, optionally 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010 or more colony-forming units per gram and/or milliliter of said inoculant composition;
- about 1×101 to about 1×1012 colony-forming units of P. bilaiae NRRL B-67157 per gram and/or milliliter of said inoculant composition, optionally 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010 or more colony-forming units per gram and/or milliliter of said inoculant composition;
- about 1×101 to about 1×1012 colony-forming units of P. bilaiae NRRL B-67158 per gram and/or milliliter of said inoculant composition, optionally 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010 or more colony-forming units per gram and/or milliliter of said inoculant composition; and/or
- about 1×101 to about 1×1012 colony-forming units of P. bilaiae NRRL B-67159 per gram and/or milliliter of said inoculant composition, optionally 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010 or more colony-forming units per gram and/or milliliter of said inoculant composition.
- 15. The inoculant composition of paragraph 12, wherein said one or more fungal strains is present in said inoculant composition in an amount effective to increase the amount/concentration of soluble phosphate in a medium that comprises insoluble phosphate, when the inoculant composition is introduced into said medium.
- 16. The inoculant composition of any one paragraphs 12-15, said composition further comprising one or more stabilizing compounds.
- 17. The inoculant composition of paragraph 16, said one or more stabilizing compounds comprising, consisting essentially of or consisting of:
- one or more monosaccharides, optionally arabinose, fructose and/or glucose;
- one or more disaccharides, optionally maltose, sucrose and/or trehalose;
- one or more maltodextrins, optionally one or more maltodextrins (e.g., one or more maltodextrins (each and/or collectively) having a DEV value of about 15 to about 20;
- one or more sugar alcohols, optionally arabitol, mannitol, sorbitol and/or xylitol;
- one or more humic acids, optionally potassium humate and/or sodium humate;
- one or more hygroscopic polymers, optionally one or more albumins, alginates, celluloses, gums (e.g., cellulose gum, guar gum, gum arabic, gum combretum, xantham gum), methyl celluloses, nylons, pectins, polyacrylic acids, polycarbonates, polyethylene glycols (PEG), polyethylenimines (PEI), polylactides, polymethylacrylates (PMA), polyurethanes, polyvinyl alcohols (PVA), polyvinylpyrrolidones (PVP), propylene glycols, sodium carboxymethyl celluloses and/or starches;
- one or more oxidation control components, optionally one or more antioxidants (e.g., ascorbic acid, ascorbyl palmitate, ascorbyl stearate, calcium ascorbate, one or more carotenoids, lipoic acid, one or more phenolic compounds (e.g., one or more flavonoids, flavones and/or flavonols), potassium ascorbate, sodium ascorbate, one or more thiols (e.g., glutathione, lipoic acid and/or N-acetyl cysteine), one or more tocopherols, one or more tocotrienols, ubiquinone and/or uric acid) and/or one or more oxygen scavengers, optionally ascorbic acid and/or sodium hydrogen carbonate; and/or
- one or more UV protectants, optionally one or more lignosulfites.
- 18. The inoculant composition of any one paragraphs 16-17, said one or more stabilizing compounds comprising about 0.0001 to about 10% (by weight) of said composition, optionally about 2 to about 6% (by weight) of said composition, optionally about 0.0005, 0.001, 0.002, 0.003, 0.004, 0.005, 0.0075, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 7, 7.5, 8, 8.5, 9, 9.5 or 10% (by weight) of said composition.
- 19. The inoculant composition of any one of paragraphs 16-18, wherein said one or more stabilizing compounds is/are present in an amount/concentration sufficient to ensure said one or more fungal strains remains viable in inoculant compositions of the present disclosure following:
- storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more;
- desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; cryopreservation at or below −80° C. for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- application;
- application and desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; and/or
- application and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
- 20. The inoculant composition of any one of paragraphs 16-18, wherein said one or more stabilizing compounds is/are present in an amount/concentration sufficient to ensure at least 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% of said one or more fungal strains remains viable following:
- storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more;
- desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more; cryopreservation at or below −80° C. for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- application;
- application and desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; and/or
- application and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
- 21. The inoculant composition of any one of paragraphs 16-18, wherein said one or more stabilizing compounds is/are present in an amount/concentration sufficient to ensure at least 1×101, 1×102, 1×103, 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010 or more colony-forming units of said one or more fungal strains per gram and/or milliliter of inoculant composition remain viable following:
- storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more;
- desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- cryopreservation at or below −80° C. for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more;
- application;
- application and desiccation by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; and/or
- application and storage at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity for a period of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more.
- 22. The inoculant composition of any one of paragraphs 12-21, said composition further comprising one or more additional microorganisms.
- 23. The inoculant composition of paragraph 22, said one or more additional microorganisms comprising, consisting essentially of or consisting of Azospirillum brasilense INTA Az-39, Bacillus amyloliquefaciens D747, Bacillus amyloliquefaciens NRRL B-50349, Bacillus amyloliquefaciens TJ1000, Bacillus amyloliquefaciens FZB24, Bacillus amyloliquefaciens FZB42, Bacillus amyloliquefaciens IN937a, Bacillus amyloliquefaciens IT-45, Bacillus amyloliquefaciens TJ1000, Bacillus amyloliquefaciens MBI600, Bacillus amyloliquefaciens BS27 (deposited as NRRL B-5015), Bacillus amyloliquefaciens BS2084 (deposited as NRRL B-50013), Bacillus amyloliquefaciens 15AP4 (deposited as ATCC PTA-6507), Bacillus amyloliquefaciens 3AP4 (deposited as ATCC PTA-6506), Bacillus amyloliquefaciens LSSA01 (deposited as NRRL B-50104), Bacillus amyloliquefaciens ABP278 (deposited as NRRL B-50634), Bacillus amyloliquefaciens 1013 (deposited as NRRL B-50509), Bacillus amyloliquefaciens 918 (deposited as NRRL B-50508), Bacillus amyloliquefaciens 22CP1 (deposited as ATCC PTA-6508) and Bacillus amyloliquefaciens BS18 (deposited as NRRL B-50633), Bacillus cereus 1-1562, Bacillus firmus 1-1582, Bacillus lichenformis BA842 (deposited as NRRL B-50516), Bacillus lichenformis BL21 (deposited as NRRL B-50134), Bacillus mycoides NRRL B-21664, Bacillus pumilus NRRL B-21662, Bacillus pumilus NRRL B-30087, Bacillus pumilus ATCC 55608, Bacillus pumilus ATCC 55609, Bacillus pumilus GB34, Bacillus pumilus KFP9F, Bacillus pumilus QST 2808, Bacillus subtilis ATCC 55078, Bacillus subtilis ATCC 55079, Bacillus subtilis MBI 600, Bacillus subtilis NRRL B-21661, Bacillus subtilis NRRL B-21665, Bacillus subtilis CX-9060, Bacillus subtilis GB03, Bacillus subtilis GB07, Bacillus subtilis QST-713, Bacillus subtilis FZB24, Bacillus subtilis D747, Bacillus subtilis 3BP5 (deposited as NRRL B-50510), Bacillus thuringiensis ATCC 13367, Bacillus thuringiensis GC-91, Bacillus thuringiensis NRRL B-21619, Bacillus thuringiensis ABTS-1857, Bacillus thuringiensis SAN 401 I, Bacillus thuringiensis ABG-6305, Bacillus thuringiensis ABG-6346, Bacillus thuringiensis AM65-52, Bacillus thuringiensis SA-12, Bacillus thuringiensis SB4, Bacillus thuringiensis ABTS-351, Bacillus thuringiensis HD-1, Bacillus thuringiensis EG 2348, Bacillus thuringiensis EG 7826, Bacillus thuringiensis EG 7841, Bacillus thuringiensis DSM 2803, Bacillus thuringiensis NB-125, Bacillus thuringiensis NB-176, BRADY, Pseudomonas jessenii PS06, Rhizobium leguminosarum SO12A-2 (IDAC 080305-01), Sinohizobium fredii CCBAU114 and/or Sinohizobium fredii USDA 205, and/or Yersinia entomophaga 082KB8.
- 24. The inoculant composition of paragraph 23, said one or more additional microorganisms comprising, consisting essentially of or consisting of Gliocladium virens ATCC 52045, Gliocladium virens GL-21, Glomus intraradices RTI-801, Metarhizium anisopliae F52, PENI, Trichoderma asperellum SKT-1, Trichoderma asperellum ICC 012, Trichoderma atroviride LC52, Trichoderma atroviride CNCM 1-1237, Trichoderma fertile JM41R, Trichoderma gamsii ICC 080, Trichoderma hamatum ATCC 52198, Trichoderma harzianum ATCC 52445, Trichoderma harzianum KRL-AG2, Trichoderma harzianum T-22, Trichoderma harzianum TH-35, Trichoderma harzianum T-39, Trichoderma harzianum ICC012, Trichoderma reesi ATCC 28217, Trichoderma virens ATCC 58678, Trichoderma virens Gl-3, Trichoderma virens GL-21, Trichoderma virens G-41, Trichoderma viridae ATCC 52440, Trichoderma viridae ICC080, and/or Trichoderma viridae TV1.
- 25. The inoculant composition of paragraph 23, said one or more additional microorganisms comprising, consisting essentially of or consisting of one or more biopesticides, optionally one or more acaricidal, insecticidal and/or nematicidal microorganisms and one or more fungicidal microorganisms.
- 26. The inoculant composition of any one of claims 22-25, said composition comprising about 1×103 to about 1×1012 colony-forming units (cfu) of said one or more additional microorganisms per gram and/or milliliter of inoculant composition, optionally about/at least 1×103, 1×104, 1×105, 1×106, 1×107, 1×108, 1×109, 1×1010, 1×1011, or 1×1012 cfu of said one or more additional microorganisms per gram and/or milliliter of inoculant composition.
- 27. The inoculant composition of any one paragraphs 12-26, wherein said composition is non-aqueous.
- 28. The inoculant composition of any one paragraphs 12-26, wherein said composition is aqueous.
- 29. The inoculant composition of any one paragraphs 12-26, wherein said composition comprises less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75 or 5% water (by weight, based upon the total weight of the composition).
- 30. The inoculant composition of any one paragraphs 12-26, wherein said composition is an amorphous liquid.
- 31. The inoculant composition of any one paragraphs 12-26, wherein said composition is an amorphous solid.
- 32. The inoculant composition of any one paragraphs 12-26, wherein said composition is a freeze-, spray- or spray-freeze-dried composition, optionally a freeze-, spray- or spray-freeze-dried powder.
- 33. A kit, comprising:
- the inoculant composition of any one of paragraphs any one of paragraphs 12-32; and
- a container housing said inoculant composition.
- 34. The kit of claim 33, said container reducing the amount of ambient light that reaches said inoculant composition by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% when sealed.
- 35. The kit of any one of paragraphs 33-34, said container reducing the amount of ambient oxygen that reaches said inoculant composition by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% when sealed.
- 36. The kit of any one of paragraphs 33-35, said container comprising, consisting essentially of, or consisting of a material having light permeability of less than about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70 or 75%.
- 37. The kit of any one of paragraphs 33-36, said container comprising, consisting essentially of, or consisting of a material having an oxygen transmission rate of less than about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, or 500 cm3/m2·day (as measured in accordance with ASTM D3985).
- 38. The kit of any one of paragraphs 33-37, said kit furthering comprising one or more oxygen-absorbing compound, optionally activated carbon, iron powder, sodium chloride, ferrous carbonate, one or more metal halide catalysts and/or sodium hydrogen carbonate.
- 39. Use of P. bilaiae NRRL 67154, P. bilaiae NRRL 67155, P. bilaiae NRRL 67156, P. bilaiae NRRL 67157, P. bilaiae NRRL 67158, and/or P. bilaiae NRRL 67159 to solubilize phosphate in a medium that comprises one or more insoluble phosphates.
- 40. A method, comprising, consisting essentially of, or consisting of:
- applying the isolated strain of any one of paragraphs 1-6, the progeny of paragraph 7, the modified microbial strain of paragraph 8, the biologically pure culture of any one of paragraphs 9-11 and/or the inoculant composition of any one of paragraphs 12-32 to a phosphate-comprising medium in an amount/concentration effective to increase the amount/concentration of soluble phosphate in said medium.
- 41. A method, comprising, consisting essentially of, or consisting of:
- introducing the isolated strain of any one of paragraphs 1-6, the progeny of paragraph 7, the modified microbial strain of paragraph 8, the biologically pure culture of any one of paragraphs 9-11 and/or the inoculant composition of any one of paragraphs 12-32 into a phosphate-comprising medium in an amount/concentration effective to increase the amount/concentration of soluble phosphate in said medium.
- Each of the isolated Penicillium bilaiae strains provided by the present disclosure—NRRL 67154, NRRL 67155, NRRL 67156, NRRL 67157, NRRL 67158 and NRRL 67159—was isolated from a soil sample collected in Canada and deposited on Dec. 2, 2015 under the terms of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure at the Agricultural Research Service Culture Collection, 1815 North University Street, Peoria, Ill. 61604, U.S.A.
- Each strain was deposited under conditions that assure access to the culture will be available during the pendency of this patent application to one determined by the Commissioner of Patents and Trademarks to be entitled thereto under 37 C.F.R. § 1.14 and 35 U.S.C. § 122. Each deposit represents a pure culture of the deposited strain.
- Each deposit is available as required by foreign patent laws in countries wherein counterparts of the subject application or its progeny are filed. However, it should be understood that the availability of a deposit does not constitute a license to practice the subject invention in derogation of patent rights granted by governmental action.
- The following examples are not intended to be a detailed catalogue of all the different ways in which the present disclosure may be implemented or of all the features that may be added to the present disclosure. Subjects skilled in the art will appreciate that numerous variations and additions to the various embodiments may be made without departing from the present disclosure. Hence, the following descriptions are intended to illustrate some particular embodiments of the invention and not to exhaustively specify all permutations, combinations and variations thereof.
- 128 soil samples were serially diluted and plated onto precipitated phosphate rose Bengal agar plates. Colonies that produced clearing zones were isolated and used to establish pure cultures of the microorganism(s) contained therein.
- Phosphate solubilization assays of 118 isolates from Example 1 were carried out in 96-well microbioreactor plates (EnzyScreen, Netherlands) in 1.5 ml of nitrate free minimal salts medium (0.1 g/l NaCl; 0.4 g/l NH4Cl; 0.1 g/l CaCl2*H2O; 1.0 g/l MgSO4*7H2O; 10.0 g/l sucrose; 5.41 g/l hydroxyapatite). The plates were inoculated from glycerol spore suspension stock plates stored at 80° C. using a 96-pin cryo-replicator that was heat sterilized and cooled before transfer. Plates were grown at room temperature (20-25° C.) and 300 rpm for 14 days. After 14 days, the plate was centrifuged for 5 minutes at 5100 rpm and 1 ml of the supernatant was transferred to a 96-well filter plate (AcroPrep Advance 96 multi-well filter plate, 1 m glass fibre, Pall Life Sciences #8231). The filter plate was placed over a 2 ml receiver plate (Whatman Uniplate 96 well round bottom, Whatman #7701-5200) and samples were filtered under vacuum using a multi-well plate vacuum manifold (Pall Life Sciences #5017). The filtered supernatants were diluted 100× with sterile water and soluble phosphate was measured using a plate reading spectrophotometer (Biotek, Winooski, Vt.) with the BioVision Phosphate Colorimetric Assay Kit (BioVision Research Products, Mountain View, Calif.) and accompanying instructions. Each isolate was grown and tested for phosphate solubilization in triplicate and the averages and standard deviations were calculated. Six of the isolates exhibited enhanced phosphate-solubilization as compared to Penicillium bilaiae ATCC-20851. Those six isolates were identified as Penicillium bilaiae and deposited under the terms of the Budapest Treaty and given the accession numbers NRRL 67154, NRRL 67155, NRRL 67156, NRRL 67157, NRRL 67158 and NRRL 67159. Table 1.
-
TABLE 1 Results of Phosphate Solubilaztion Assay Increase in solubilized phosphate Strain (as compared to ATCC-20851) NRRL 67154 30.86% increase NRRL 67155 28.16% increase NRRL 67156 10.35% increase NRRL 67157 10.56% increase NRRL 67158 15.65% increase NRRL 67159 16.5% increase - Acinetobacter, Actinomycetes, Aegerita, Agrobacterium (e.g., A. radiobacter strains such as K1026 and K84), Akanthomyces, Alcaligenes, Alternaria, Aminobacter (e.g., A. aganoensis, A. aminovorans, A. anthyllidis, A. ciceronei, A. lissarensis, A. niigataensis), Ampelomyces (e.g., A. quisqualis strains such as M-10), Anabaena (e.g., A. aequalis, A. affinis, A. angstumalis angstumalis, A. angstumalis marchita, A. aphanizomendoides, A. azollae, A. bornetiana, A. catenula, A. cedrorum, A. circinalis, A. confervoides, A. constricta, A. cyanobacterium, A. cycadeae, A. cylindrica, A. echinispora, A. felisii, A. fos-aquae fos-aquae, A. fos-aquae minor, A. fos-aquae treleasei, A. helicoidea, A. inaequalis, A. lapponica, A. laxa, A. lemmermannii, A. levanderi, A. limnetica, A. macrospora macrospora, A. macrospora robusta, A. monticulosa, A. nostoc, A. ascillarioides, A. planctonica, A. raciborski, A. scheremetievi, A. sphaerica, A. spiroides crassa, A. spiroides sprroides, A. subcylindrica, A. torulosa, A. unispora, A. variabilis, A. verrucosa, A. viguieri, A. wisconsinense, A. zierlingii), Arthrobacter, Arthrobotrys (e.g., A. aggregata, A. alaskana, A. ameropora, A. anomala, A. apscheronica, A. arthrobotryoides, A. azerbaijanica, A. bakunika, A. botryospora, A. brochopaga, A. chazarica, A. chilensis, A. cladodes, A. calvispora, A. compacta, A. conoides, A. constringens, A. cylindrospora, A. dactyloides, A. deflectans, A. dendroides, A. doliformis, A. drechsleri, A. elegans, A. ellipsospora, A. entomopaga, A. ferox, A. foliicola, A. fruticulosa, A. globospora, A. hatospora, A. hertziana, A. indica, A. irregularis, A. javanica, A. kirghizica, A. longa, A. longiphora, A. longiramulifera, A. longispora, A. mangrovispora, A. megaspora, A. microscaphoides, A. microspora, A. multisecundaria, A. musiformis, A. nematopaga, A. nonseptata, A. oligospora, A. oudemansii, A. oviformis, A. perpasta, A. polycephala, A. pseudoclavata, A. pyriformis, A. recta, A. robusta, A. rosea, A. scaphoides, A. sclerohypha, A. shahriari, A. shizishanna, A. sinensis, A. soprunovii, A. stilbacea, A. straminicola, A. superba, A. tabrizica, A. venusta, A. vermicola, A. yunnanensis), Aschersonia, Ascophaera, Aspergillus (e.g., A. flavus strains such as NRRL 21882, A. parasiticus), Aulosira (e.g., A. aenigmatica, A. africana, A. bohemensis, A. bombayensis, A. confluens, A. fertilissima, A. fertilissma var. tenius, A. fritschii, A. godoyana, A. implexa, A. laxa, A. plantonica, A. prolfica, A. pseuodoramosa, A. schauinslandii, A. striata, A. terrestris, A. thermalis), Aureobacterium, Aureobasidium (e.g., A. pullulans strains such as DSM 14940 and DSM 14941), Azobacter, Azorhizobium (e.g., A. caulinodans, A. doebereinerae, A. oxalatiphilum), Azospirillum (e.g., A. amazonense strains such as BR 11140 (SpY2T), A. brasilense strains such as INTA Az-39, AZ39, XOH, BR 11002, BR 11005, Ab-V5 and Ab-V6, A. canadense, A. doebereinerae, A. formosense, A. halopraeferans, A. irakense, A. largimobile, A. lipoferum strains such as BR 11646, A. melinis, A. oryzae, A. picis, A. rugosum, A. thiophilum, A. zeae), Azotobacter (e.g., A. agilis, A. armeniacus, A. sp. AR, A. beijerinckii, A. chroococcum, A. DCU26, A. FA8, A. nigricans, A. paspali, A. salinestris, A. tropicalis, A. vinelandii), Bacillus (e.g., B. amyloliquefaciens strains such as D747, NRRL B-50349, TJ1000 (also known as 1BE, isolate ATCC BAA-390), FZB24, FZB42, IN937a, IT-45, TJ1000, MBI600, BS27 (deposited as NRRL B-5015), BS2084 (deposited as NRRL B-50013), 15AP4 (deposited as ATCC PTA-6507), 3AP4 (deposited as ATCC PTA-6506), LSSA01 (deposited as NRRL B-50104), ABP278 (deposited as NRRL B-50634), 1013 (deposited as NRRL B-50509), 918 (deposited as NRRL B-50508), 22CP1 (deposited as ATCC PTA-6508) and BS18 (deposited as NRRL B-50633), B. cereus strains such as I-1562, B. firmus strains such as I-1582, B. laevolacticus, B. lichenformis strains such as BA842 (deposited as NRRL B-50516) and BL21 (deposited as NRRL B-50134), B. macerns, B. firmus, B. mycoides strains such as NRRL B-21664, B. pasteurii, B. pumilus strains such as NRRL B-21662, NRRL B-30087, ATCC 55608, ATCC 55609, GB34, KFP9F and QST 2808, B. sphaericus, B. subtilis strains such as ATCC 55078, ATCC 55079, MBI 600, NRRL B-21661, NRRL B-21665, CX-9060, GB03, GB07, QST 713, FZB24, D747 and 3BP5 (deposited as NRRL B-50510), B. thuringiensis strains such as ATCC 13367, GC-91, NRRL B-21619, ABTS-1857, SAN 401 I, ABG-6305, ABG-6346, AM65-52, SA-12, SB4, ABTS-351, HD-1, EG 2348, EG 7826, EG 7841, DSM 2803, NB-125 and NB-176), Beijerinckia, Beauveria (e.g., B. bassiana strains such as ATCC 26851, ATCC 48023, ATCC 48585, ATCC 74040, ATCC-74250, DSM 12256 and PPRI 5339), Beijerinckia, Blastodendrion, Bosea (e.g., B. eneae, B. lathyri, B. lupini, B. massiliensis, B. minatitlanensis, B. robiniae, B. thiooxidans, B. vestrisii), Bradyrhizobium (e.g., B. arachidis, B. bete, B. canariense, B. cytisi, B. daqingense, B. denitrificans, B. diazoefficiens, B. elkanii strains such as SEMIA 501, SEMIA 587 and SEMIA 5019, B. ganzhouense, B. huanghuauhaiense, B. icense, B. ingae, B. iriomotense, B. japonicum strains such as NRRL B-50586 (also deposited as NRRL B-59565), NRRL B-50587 (also deposited as NRRL B-59566), NRRL B-50588 (also deposited as NRRL B-59567), NRRL B-50589 (also deposited as NRRL B-59568), NRRL B-50590 (also deposited as NRRL B-59569), NRRL B-50591 (also deposited as NRRL B-59570), NRRL B-50592 (also deposited as NRRL B-59571), NRRL B-50593 (also deposited as NRRL B-59572), NRRL B-50594 (also deposited as NRRL B-50493), NRRL B-50608, NRRL B-50609, NRRL B-50610, NRRL B-50611, NRRL B-50612, NRRL B-50726, NRRL B-50727, NRRL B-50728, NRRL B-50729, NRRL B-50730, SEMIA 566, SEMIA 5079, SEMIA 5080, USDA 6, USDA 110, USDA 122, USDA 123, USDA 127, USDA 129 and USDA 532C, B. jicamae, B. lablabi, B. liaoningense, B. manausense, B. neotropicale, B. oligotrophicum, B. ottawaense, B. pachyrhizi, B. paxllaeri, B. retamae, B. rifense, B. valentinum, B. yuanmingense), Burkholderia (e.g., B. acidipaludis, B. ambifaria, B. andropogonis, B. anthina, B. arboris, B. bannensis, B. bryophila, B. caledonica, B. caribensis, B. caryophylli, B. cenocepacua, B. choica, B. cocovenenans, B. contaminans, B. denitrificans, B. diazotrophica, B. diffusa, B. dilworthii, B. dolosa, B. eburnea, B. endofungorum, B. ferrariae, B. fungorum, B. ginsengisoli, B. gladioli, B. glathei, B. glumae, B. graminis, B. grimmiae, B. heleia, B. hospital, B. humi, B. kururiensis, B. lata, B. latens, B. mallei, B. megapolitana, B. metallica, B. mimosarum, B. multivorans, B. nodosa, B. norimbergensis, B. oklahomensis, B. phenazinium, B. phenoliruptrix, B. phymatum, B. phytofirmans, B. pickettii, B. plantarii, B. pseudomallei, B. pseudomultivorans, B. pyrrocinia, B. rhizoxinica, B. rhynchosiae, B. sabiae, B. sacchari, B. sartisoli, B. sediminicola, B. seminalis, B. silvatlantica, B. singaporensis, B. soli, B. sordidcola, B. sp. strains such as A396, B. sprentiae, B. stabilis, B. symbiotica, B. telluris, B. terrae, B. terrestris, B. terricola, B. thailandensis, B. tropica, B. tuberum, B. ubonensis, B. udeis, B. unamae, B. vandii, B. vietnamiensis, B. xenovorans, B. zhejiangensis), Brevibacillus, Burkholderia (e.g., B. sp. A396 nov. rinojensis NRRL B-50319), Calonectria, Candida (e.g., C. oleophila such I-182, C. saitoana), Candidatus (e.g., C. Burkholderia calva, C. Burkholderia crenata, C. Burkholderia hispidae, C. Burkholderia kirkii, C. Burkholderia mamillata, C. Burkholderia nigropunctata, C. Burkholderia rigidae, C. Burkholderia schumannianae, C. Burkholderia verschuerenii, C. Burkholderia virens, C. Phytoplasma allocasuarinae, C. Phytoplasma americanum, C. Phytoplasma asteris, C. Phytoplasma aurantifolia, C. Phytoplasma australiense, C. Phytoplasma balanitae, C. Phytoplasma brasiliense, C. Phytoplasma caricae, C. Phytoplasma castaneae, C. Phytoplasma cocosnigeriae, C. Phytoplasma cocostanzaniae, C. Phytoplasma convolvuli, C. Phytoplasma costaricanum, C. Phytoplasma cynodontis, C. Phytoplasma fragariae, C. Phytoplasma fraxini, C. Phytoplasma graminis, C. Phytoplasma japonicum, C. Phytoplasma luffae, C. Phytoplasma lycopersici, C. Phytoplasma malasianum, C. Phytoplasma mali, C. Phytoplasma omanense, C. Phytoplasma oryzae, C. Phytoplasma palmae, C. Phytoplasma palmicola, C. Phytoplasma phoenicium, C. Phytoplasma pini, C. Phytoplasma pruni, C. Phytoplasma prunorum, C. Phytoplasma pyri, C. Phytoplasma rhamni, C. Phytoplasma rubi, C. Phytoplasma solani, C. Phytoplasma spartii, C. Phytoplasma sudamericanum, C. Phytoplasma tamaricis, C. Phytoplasma trifolii, C. Phytoplasma ulmi, C. Phytoplasma vitis, C. Phytoplasma ziziphi), Chromobacterium (e.g., C. subtsugae NRRL B-30655 and PRAA4-1, C. vaccinia strains such as NRRL B-50880, C. violaceum), Chryseomonas, Clavibacter, Clonostachys (e.g., C. rosea f. catenulata (also referred to as Gliocladium catenulatum) strains such as J1446), Clostridium, Coelemomyces, Coelomycidium, Colletotrichum (e.g., C. gloeosporioides strains such as ATCC 52634), Comomonas, Conidiobolus, Coniothyrium (e.g., C. minitans strains such as CON/M191-08), Cordyceps, Corynebacterium, Couchia, Cryphonectria (e.g., C. parasitica), Cryptococcus (e.g., C. albidus), Cryptophlebia (e.g., C. leucotreta), Culicinomyces, Cupriavidus (e.g., C. alkaliphilus, C. basilensis, C. campinensis, C. gilardii, C. laharis, C. metallidurans, C. numazuensis, C. oxalaticus, C. pampae, C. pauculus, C. pinatubonensis, C. respiraculi, C. taiwanensis), Curtobacterium, Cydia (e.g., C. pomonella strains such as V03 and V22), Dactylaria (e.g., D. candida), Delftia (e.g., D. acidovorans strains such as RAY209), Desulforibtio, Desulfovibrio, Devosia (e.g., D. neptuniae), Dilophosphora (e.g., D. alopecuri), Engyodontium, Enterobacter, Entomophaga, Entomophthora, Erynia, Escherichia (e.g., E. intermedia), Eupenicillium, Exiguobacaterium, Filariomyces, Filobasidiella, Flavobacterium (e.g., F. H492 NRRL B-50584), Frankia (e.g., F. alni), Fusarium (e.g., F. laterium, F. oxysporum, F. solani), Gibellula, Gigaspora (e.g., G. margarita), Gliocladium (e.g., G. virens strains such as ATCC 52045 and GL-21), Glomus (e.g., G. aggregatum, G. brasilianum, G. clarum, G. deserticola, G. etunicatum, G. fasciculatum, G. intraradices strains such as RTI-801, G. monosporum, G. mosseae), Gluconobacter, Halospirulina, Harposporium (e.g., H. anguillulae), Hesperomyces, Hirsutella (e.g., H. minnesotensis, H. rhossiliensis, H. thomsonii strains such as ATCC 24874), Hydrogenophage, Hymenoscyphous (e.g., H. ericae), Hymenostilbe, Hypocrella, Isaria (e.g., I. fumosorosea strains such as Apopka-97 (deposited as ATCC 20874)), Klebsiella (e.g., K. pneumoniae, K. oxytoca), Kluyvera, Laccaria (e.g., L. bicolor, L. laccata), Lactobacillus, Lagenidium, Lecanicillium (e.g., L. lecanii strains such as KVO1, L. longisporum strains such as KV42 and KV71), Leptolegnia, Lysobacter (e.g., L. antibioticus strains such as 13-1 and HS124, L. enzymogenes strains such as 3.1T8), Massospora, Meristacrum (e.g., M. asterospermum), Mesorhizobium (e.g., M. abyssinicae, M. albiziae, M. alhagi, M. amorphae, M. australicum, M. camelthorni, M. caraganae, M. chacoense, M. ciceri, M. gobiense, M. hawassense, M. huakuii, M. loti, M. mediterraneum, M. metallidurans, M. muleiense, M. opportunistum, M. plurifarium, M. qingshengii, M. robiniae, M. sangaii, M. septentrionale, M. shangrilense, M. shonense, M. silamurunense, M. tamadayense, M. tarimense, M. temperatum, M. thiogangeticum, M. tianshanense), Metarhizium (e.g., M. anisopliae (also referred to as M. brunneum, Metarrhizium anisopliae, and green muscadine) strains such as IMI 330189, FI-985, FI-1045, F52 (deposited as DSM 3884, DSM 3885, ATCC 90448, SD 170 and ARSEF 7711) and ICIPE 69), M. flavoviride strains such as ATCC 32969), Methylobacterium (e.g., M. adhaesivum, M. aerolatum, M. aminovorans, M. aquaticum, M. brachiatum, M. brachythecii, M. bullatum, M. cerastii, M. chloromethanicum, M. dankookense, M. dichloromethanicum, M. extorquens, M. fujisawaense, M. gnaphalii, M. goesingense, M. gossipiicola, M. gregans, M. haplocladii, M. hispanicum, M. iners, M. isbiliense, M. jeotgali, M. komagatae, M. longum, M. lusitanum, M. marchantiae, M. mesophilicum, M. nodulans, M. organophilum, M. oryzae, M. oxalidis, M. persicinum, M. phyllosphaerae, M. platani, M. podarium, M. populi, M. radiotolerans, M. rhodesianum, M. rhodinum, M. salsuginis, M. soli, M. suomiense, M. tardum, M. tarhaniae, M. thiocyanatum, M. thurigiense, M. trifolii, M. variabile, M. zatmanii), Metschnikowia (e.g., M. fructicola), Microbacterium (e.g., M. laevaniformans), Microdochium (e.g., M. dimerum), Microsphaeropsis (e.g., M. ochracea P130A), Microvirga (e.g., M. aerilata, M. aerophila, M. flocculans, M. guangxiensis, M. lotononidis, M. lupini, M. subterranea, M. vignae, M. zambiensis), Monacrosporium (e.g., M. cionopagum), Mucor, Muscodor (e.g., M. albus such NRRL 30547, QST 20799 and SA-13, M. roseus strains such as NRRL 30548), Mycoderma, Myiophagus, Myriangium, Myrothecium (e.g., M. verrucaria), Nectria, Nematoctonus (e.g., N. geogenius, N. leiosporus), Neozygites, Nomuraea (e.g., N. rileyi strains such as SA86101, GU87401, SR86151, CG128 and VA9101), Nostoc (e.g., N. azollae, N. caeruleum, N. carneum, N. comminutum, N. commune, N. ellipsosporum, N. flagelliforme, N. linckia, N. longstaffi, N. microscopicum, N. muscorum, N. paludosum, N. pruniforme, N. punctifrome, N. sphaericum, N. sphaeroides, N. spongiaeforme, N. verrucosum), Ochrobactrum (e.g., O. anthropi, O. cicero, O. cytisi, O. daejeonense, O. gallinmfaecis, O. grigonense, O. guangzhouense, I. haematophilum, O. intermedium, O. lupini, O. oryzae, O. pectoris, O. pituitosum, O. pseudointermedium, I. pseudogrignonense, O. rhizosphaerae, O. thiophenivorans, O. tritici), Oidiodendron, Paecilomyces (e.g., P. fumosoroseus strains such as FE991 and FE 9901, P. lilacinus strains such as 251, DSM 15169 and BCP2), Paenibacillus (e.g., P. alvei strains such as NAS6G6, P. azotofixans, P. polymyxa strains such as ABP166 (deposited as NRRL B-50211)), Pandora, Pantoea (e.g., P. agglomerans strains such as NRRL B-21856, P. vagans strains such as C9-1), Paraglomus (e.g., P. brazilianum), Paraisaria, Pasteuria, Pasteuria (e.g., P. nishizawae strains such as Pn1, P. penetrans, P. ramose, P. sp. strains such as ATCC PTA-9643 and ATCC SD-5832, P. thornea, P. usage), Penicillium (e.g., P. albidum, P. aurantiogriseum, P. bilaiae (formerly known as P. bilaii and P. bilaji) strains such as ATCC 18309, ATCC 20851, ATCC 22348, NRRL 50162, NRRL 50169, NRRL 50776, NRRL 50777, NRRL 50778, NRRL 50777, NRRL 50778, NRRL 50779, NRRL 50780, NRRL 50781, NRRL 50782, NRRL 50783, NRRL 50784, NRRL 50785, NRRL 50786, NRRL 50787, NRRL 50788 and RS7B-SD1, P. brevicompactum strains such as AgRF18, P. canescens strains such as ATCC 10419, P. chyrsogenum, P. citreonigrum, P. citrinum, P. digitatum, P. expansum strains such as ATCC 24692 and YT02, P. fellatanum strains such as ATCC 48694, P. frequentas, P. fuscum, P. fussiporus, P. gaestrivorus strains such as NRRL 50170, P. glabrum strains such as DAOM 239074 and CBS 229.28, P. glaucum, P. griseofulvum, P. implicatum, P. janthinellum strains such as ATCC 10455, P. lanosocoeruleum strains such as ATCC 48919, P. lilacinum, P. minioluteum, P. montanense, P. nigricans, P. oxalicum, P. pinetorum, P. pinophilum, P. purpurogenum, P. radicum strains such as ATCC 201836, FRR 4717, FRR 4719 and N93/47267, P. raistrickii strains such as ATCC 10490, P. rugulosum, P. simplicissimum, P. solitum, P. variabile, P. velutinum, P. viridicatum), Phingobacterium, Phlebiopsis (e.g., P. gigantea), Photorhabdus, Phyllobacterium (e.g., P. bourgognense, P. brassicacearum, P. catacumbae, P. endophyticum, P. ifriqiyense, P. leguminum, P. loti, P. myrsinacearum, P. sophorae, P. trifolii), Pichia (e.g., P. anomala strains such as WRL-076), Pisolithus (e.g., P. tinctorius), Planktothricoides, Plectonema, Pleurodesmospora, Pochonia (e.g., P. chlamydopora), Podonectria, Polycephalomyces, Prochlorocoous (e.g., P. marinus), Prochloron (e.g., P. didemni), Prochlorothrix, Pseudogibellula, Pseudomonas (e.g., P. agarici, P. antartica, P. aurantiaca, P. aureofaciens, P. azotifigens, P. azotoformans, P. balearica, P. blatchfordae, P. brassicacearum, P. brenneri, P. cannabina, P. cedrina, P. cepacia, P. chlororaphis strains such as MA 342, P. congelans, P. corrugata, P. costantinii, P. denitrificans, P. entomophila, P. fluorescens strains such as ATCC 27663, CL 145A and A506, P. fragii, P. fuscovaginae, P. fulva, P. gessardii, P. jessenii strains such as PS06, P. kilonensis, P. koreensis, P. libanensis, P. lii, P. lundensis, P. lutea, P. luteola, P. mandelii, P. marginalis, P. meditrranea, P. meridana, P. migulae, P. moraviensis, P. mucidolens, P. orientalis, P. oryzihabitans, P. palleroniana, P. panacis, P. parafulva, P. peli, P. pertucinogena, P. plecoglossicida, P. protogens, P. proteolytica, P. putida, P. pyrocina strains such as ATCC 15958, P. rhodesiae, P. sp. strains such as DSM 13134, P. striata, P. stutzeri, P. syringae, P. synxantha, P. taetrolens, P. thisvervalensis, P. tolaasii, P. veronii), Pseudozyma (e.g., P. flocculosa strains such as PF-A22 UL), Pythium (e.g., P. oligandrum strains such as DV 74), Rhizobium (e.g., R. aggregatum, R. alamii, R. alkalisoli, P. alvei, P. azibense, P. borbori, R. calliandrae, R. cauense, R. cellulosilyticum, R. daejeonense, R. endolithicum, R. endophyticum, R. etli, R. fabae, R. flavum, R. fredii, R. freirei, R. galegae, R. gallicum, R. giardinii, R. grahamii, R. hainanense, R. halophytocola, R. halotolerans, R. helanshanense, R. herbae, R. huautlense, R. indigoferae, R. jaguaris, R. kunmingense, R. laguerreae, R. larrymoorei, R. leguminosarum strains such as SO12A-2 (IDAC 080305-01), R. lemnae, R. leucaenae, R. loessense, R. lupini, R. lusitanum, R. mayense, R. mesoamericanum, R. mesosinicum, R. miluonense, R. mongolense, R. multihospitium, R. naphthalenivorans, R. nepotum, R. oryzae, R. pakistanensis, R. paknamense, R. paranaense, R. petrolearium, R. phaseoli, R. phenanthrenilyticum, R. pisi, R. pongamiae, R. populi, R. pseudoryzae, R. pusense, R. qilianshanese, r. radiobacter, R. rhizogenes, R. rhizoryzae, R. rozettiformans, R. rubi, R. selenitireeducens, R. skierneiwicense, R. smilacinae, R. soli, R. sophorae, R. sophoriradicis, R. sphaerophysae, R. straminoryzae, R. subbaraonis, R. sullae, R. taibaishanense, R. tarimense, R. tibeticum, R. trifolii strains such as RP113-7, R. tropici strains such as SEMIA 4080, R. tubonense, R. undicola, R. vallis, R. viciae strains such as P1NP3Cst, SU303 and WSM 1455, R. vignae, R. vitis, R. yanglingense, R. yantingense), Rhizoctonia, Rhizopogon (e.g., R. amylopogon, R. fulvigleba, R. luteolus, R. villosuli), Rhodococcus, Saccharopolyspora (e.g., S. spinosa), Scleroderma (e.g., S. cepa S. citrinum), Septobasidium, Serratia, Shinella (e.g., S. kummerowiae), Sinorhizoium (e.g., S. abri, S. adhaerens, S. americanum, S. arboris, S. chiapanecum, S. fredii strains such as CCBAU114 and USDA 205, S. garamanticus, S. indiaense, S. kostiense, S. kummerowiae, S. medicae, S. meliloti strains such as MSDJ0848, S. mexicanus, S. numidicus, S. psoraleae, S. saheli, S. sesbaniae, S. sojae, S. terangae, S. xinjiangense), Sorosporella, Sphaerodes (e.g., S. mycoparasitica strains such as IDAC 301008-01), Spodoptera (e.g., S. littoralis), Sporodiniella, Steinernema (e.g., S. carpocapsae, S. feltiae, S. kraussei strains such as L137), Stenotrophomonas, Streptomyces (e.g., S. NRRL B-30145, S. M1064, S. WYE 53 (deposited as ATCC 55750), S. cacaoi strains such as ATCC 19093, S. galbus strains such as NRRL 30232, S. griseoviridis strains such as K61, S. lydicus strains such as WYEC 108 (deposited as ATCC 55445), S. violaceusniger strains such as YCED-9 (deposited as ATCC 55660)), Streptosporangium, Stillbella, Swaminathania, Talaromyces (e.g., T. aculeatus, T. flavus strains such as VI17b), Tetranacrium, Thiobacillus, Tilachlidium, Tolypocladium, Tolypothrix, Torrubiella, Torulospora, Trenomyces, Trichoderma (e.g. T. asperellum strains such as SKT-1, T. atroviride strains such as LC52 and CNCM 1-1237, T. fertile strains such as JM41R, T. gamsii strains such as ICC 080, T. hamatum strains such as ATCC 52198, T. harzianum strains such as ATCC 52445, KRL-AG2, T-22, TH-35, T-39 and ICC012, T. polysporum, T. reesi strains such as ATCC 28217 T. stromaticum, T. virens strains such as ATCC 58678, GL-3, GL-21 and G-41, T. viridae strains such as ATCC 52440, ICC080 and TV1), Typhula, Ulocladium (e.g., U. oudemansii strains such as HRU3), Uredinella, Variovorax, Verticillium (e.g., V. chlamydosporum, V. lecanii strains such as ATCC 46578), Vibrio, Xanthobacter, Xanthomonas. Xenorhadbus, Yersinia (e.g., Y. entomophaga strains such as 082KB8), Zoophthora
Claims (20)
1. A non-naturally occurring composition comprising the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67154 and/or the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67155 and/or the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67156 and/or the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67157 and/or the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67158 and/or the isolated Penicillium bilaiae strain having the deposit accession number NRRL 67159 and/or a modified microbial strain derived from one of the aforementioned Penicillium bilaiae strains in a synthetic carrier.
2. The non-naturally occurring composition of claim 1 , wherein said synthetic carrier is aqueous.
3. The non-naturally occurring composition of claim 1 , wherein said synthetic carrier is non-aqueous.
4. The non-naturally occurring composition of claim 1 , wherein said synthetic carrier comprises one or more hygroscopic polymers.
5. The non-naturally occurring composition of claim 1 , wherein said synthetic carrier comprises one or more maltodextrins.
6. The non-naturally occurring composition of claim 1 , wherein said synthetic carrier comprises one or more monosaccharides.
7. The non-naturally occurring composition of claim 1 , wherein said synthetic carrier comprises one or more disaccharides.
8. The non-naturally occurring composition of claim 1 , wherein said synthetic carrier comprises one or more sugar alcohols.
9. The non-naturally occurring composition of claim 1 , wherein said synthetic carrier comprises one or more humic acids.
10. The non-naturally occurring composition of claim 1 , wherein said synthetic carrier comprises one or more fulvic acids.
11. The non-naturally occurring composition of claim 1 , wherein said synthetic carrier comprises one or more vinylpyrrolidone polymers.
12. The non-naturally occurring composition of claim 1 , wherein said synthetic carrier comprises one or more alcohol ethyoxylates.
13. The non-naturally occurring composition of claim 1 , wherein said synthetic carrier comprises one or more naphthalene sulfonates.
14. The non-naturally occurring composition of claim 1 , wherein said synthetic carrier comprises one or more peats and/or peat extracts.
15. The non-naturally occurring composition of claim 1 , further comprising one or more lipo-chitooligosaccharides.
16. The non-naturally occurring composition of claim 1 , further comprising one or more chitin oligomers.
17. The non-naturally occurring composition of claim 1 , comprising at least 1×104 colony-forming units of said Penicillium bilaiae strain having the deposit accession number NRRL 67154 and/or Penicillium bilaiae strain having the deposit accession number NRRL 67155 and/or Penicillium bilaiae strain having the deposit accession number NRRL 67156 and/or Penicillium bilaiae strain having the deposit accession number NRRL 67157 and/or Penicillium bilaiae strain having the deposit accession number NRRL 67158 and/or Penicillium bilaiae strain having the deposit accession number NRRL 67159 and/or modified microbial strain per gram and/or milliliter of said composition.
18. The non-naturally occurring composition of claim 1 , comprising at least 1×107 colony-forming units of said Penicillium bilaiae strain having the deposit accession number NRRL 67154 and/or Penicillium bilaiae strain having the deposit accession number NRRL 67155 and/or Penicillium bilaiae strain having the deposit accession number NRRL 67156 and/or Penicillium bilaiae strain having the deposit accession number NRRL 67157 and/or Penicillium bilaiae strain having the deposit accession number NRRL 67158 and/or Penicillium bilaiae strain having the deposit accession number NRRL 67159 and/or modified microbial strain per gram and/or milliliter of said composition.
19. A non-naturally occurring composition comprising a plant propagation material that is at least partially coated with the non-naturally occurring composition of claim 1 .
20. The non-naturally occurring composition of claim 19 , wherein said plant propagation material is a seed.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US17/673,395 US20220167630A1 (en) | 2016-01-28 | 2022-02-16 | Phosphate-solubilzing fungal strains |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662287965P | 2016-01-28 | 2016-01-28 | |
PCT/US2017/013416 WO2017131971A1 (en) | 2016-01-28 | 2017-01-13 | Phosphate-solubilzing fungal strains |
US201816073050A | 2018-07-26 | 2018-07-26 | |
US17/673,395 US20220167630A1 (en) | 2016-01-28 | 2022-02-16 | Phosphate-solubilzing fungal strains |
Related Parent Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2017/013416 Division WO2017131971A1 (en) | 2016-01-28 | 2017-01-13 | Phosphate-solubilzing fungal strains |
US16/073,050 Division US11259526B2 (en) | 2016-01-28 | 2017-01-13 | Phosphate-solubilzing fungal strains |
Publications (1)
Publication Number | Publication Date |
---|---|
US20220167630A1 true US20220167630A1 (en) | 2022-06-02 |
Family
ID=58010377
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US16/073,050 Active 2037-05-26 US11259526B2 (en) | 2016-01-28 | 2017-01-13 | Phosphate-solubilzing fungal strains |
US17/673,395 Abandoned US20220167630A1 (en) | 2016-01-28 | 2022-02-16 | Phosphate-solubilzing fungal strains |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US16/073,050 Active 2037-05-26 US11259526B2 (en) | 2016-01-28 | 2017-01-13 | Phosphate-solubilzing fungal strains |
Country Status (7)
Country | Link |
---|---|
US (2) | US11259526B2 (en) |
EP (1) | EP3408414B1 (en) |
AR (1) | AR107475A1 (en) |
BR (1) | BR112018015312A2 (en) |
CA (1) | CA3010031A1 (en) |
UY (1) | UY37091A (en) |
WO (1) | WO2017131971A1 (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA3097942A1 (en) | 2018-05-07 | 2019-11-14 | Novozymes Bioag A/S | Microbacterium isolates and uses thereof |
CN109628317A (en) * | 2018-06-20 | 2019-04-16 | 贵州大学 | A kind of mould and its application with degradation slightly solubility Phos function |
WO2020263734A1 (en) | 2019-06-24 | 2020-12-30 | Novozymes Bioag A/S | Erwinia isolates and uses thereof |
WO2021086695A1 (en) | 2019-10-29 | 2021-05-06 | Novozymes Bioag A/S | Microbacterium isolates and uses thereof |
WO2021101937A1 (en) | 2019-11-20 | 2021-05-27 | Novozymes Bioag A/S | Pseudomonas isolates and uses thereof |
WO2021101949A1 (en) | 2019-11-22 | 2021-05-27 | Novozymes Bioag A/S | Paenibacillus isolates and uses thereof |
US11530169B1 (en) | 2021-10-07 | 2022-12-20 | Bio-Soil Enhancers, Inc. | Fertilizer microbe combination |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0284236B1 (en) * | 1987-03-17 | 1991-08-21 | Her Majesty in Right of Canada as represented by the Minister of Agriculture Canada | Methods and compositions for increasing the amounts of phosphorous and/or micronutrients available for plant uptake from soils |
US5484464A (en) | 1993-12-29 | 1996-01-16 | Philom Bios, Inc.. | Methods and compositions for increasing the benefits of rhizobium inoculation to legume crop productivity |
US7241588B2 (en) | 2003-09-09 | 2007-07-10 | Grape King Inc. | Method for producing novel β-lactam antibiotic from protoplast fusion strain |
FI119597B (en) * | 2004-12-31 | 2009-01-15 | Verdera Oy | Stable microbial inoculants and processes for their preparation |
AR073718A1 (en) | 2008-10-01 | 2010-11-24 | Novozymes Biolog Ltd | METHODS AND COMPOSITIONS TO INCREASE THE AMOUNTS OF PHOSPHORY AVAILABLE FOR THE SOIL PLANT ABSORPTION |
KR101903054B1 (en) | 2012-07-11 | 2018-10-02 | 삼성디스플레이 주식회사 | Organic light emitting display apparatus and the method for manufacturing the same |
US9365464B2 (en) * | 2012-11-16 | 2016-06-14 | Novozymes Bioag A/S | Microbial strains, compositions, and methods for increasing available phosphate for plants |
-
2017
- 2017-01-13 WO PCT/US2017/013416 patent/WO2017131971A1/en active Application Filing
- 2017-01-13 US US16/073,050 patent/US11259526B2/en active Active
- 2017-01-13 EP EP17704344.5A patent/EP3408414B1/en active Active
- 2017-01-13 BR BR112018015312A patent/BR112018015312A2/en unknown
- 2017-01-13 CA CA3010031A patent/CA3010031A1/en active Pending
- 2017-01-25 UY UY0001037091A patent/UY37091A/en not_active Application Discontinuation
- 2017-01-27 AR ARP170100224A patent/AR107475A1/en unknown
-
2022
- 2022-02-16 US US17/673,395 patent/US20220167630A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US11259526B2 (en) | 2022-03-01 |
BR112018015312A2 (en) | 2018-12-18 |
AR107475A1 (en) | 2018-05-02 |
UY37091A (en) | 2017-08-31 |
CA3010031A1 (en) | 2017-08-03 |
EP3408414A1 (en) | 2018-12-05 |
US20190029267A1 (en) | 2019-01-31 |
EP3408414B1 (en) | 2020-12-16 |
WO2017131971A1 (en) | 2017-08-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11259526B2 (en) | Phosphate-solubilzing fungal strains | |
RU2730863C2 (en) | Stable compositions with inoculant and methods for production thereof | |
US20230227770A1 (en) | Bacillus Isolates and Uses Thereof | |
US11963531B2 (en) | Microbial compositions and methods | |
US9788551B2 (en) | Liquid preparation for biological plant protection, method for producing it and use thereof | |
US20200315183A1 (en) | Stable inoculant compositions and methods for producing same | |
EP3462880A1 (en) | Stable liquid inoculant compositions and coated plant propagation materials comprising same | |
AU2018211904A1 (en) | Method of promoting bacillus spore germination | |
RU2016147388A (en) | COMBINATIONS OF ACTIVE COMPOUNDS | |
EP3558003A1 (en) | Stable inoculant compositions and methods for producing same | |
US20190320661A1 (en) | Microbial compositions and methods | |
EP3629740A1 (en) | Stable inoculant compositions comprising methylated plant oils | |
US20200093125A1 (en) | Stable inoculant compositions comprising paraffin oils/waxes | |
WO2018218008A1 (en) | Stable liquid inoculant compositions comprising dodecane | |
WO2021101937A1 (en) | Pseudomonas isolates and uses thereof | |
US20210251236A1 (en) | Microbacterium isolates and uses thereof | |
Önneby et al. | Reduced leaching of the herbicide MCPA after bioaugmentation with a formulated and stored Sphingobium sp. | |
BR112020003782A2 (en) | methods to detect microbes | |
JP2022539317A (en) | Erwinia isolates and their uses | |
WO2021086695A1 (en) | Microbacterium isolates and uses thereof | |
NZ791406A (en) | Method of promoting bacillus spore germination |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
STPP | Information on status: patent application and granting procedure in general |
Free format text: DOCKETED NEW CASE - READY FOR EXAMINATION |
|
STPP | Information on status: patent application and granting procedure in general |
Free format text: NON FINAL ACTION MAILED |
|
STPP | Information on status: patent application and granting procedure in general |
Free format text: NON FINAL ACTION MAILED |
|
STCB | Information on status: application discontinuation |
Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION |