JP2022539317A - Erwinia isolates and their uses - Google Patents

Abstract

The present disclosure provides fungicidal Erwinina and Pseudomonas strains useful for protecting plants and improving crop yields, as well as said isolates, cell extracts obtained from said isolates and a cell filtrate obtained from a culture of said isolate. [Selection figure] None

Description

[Reference to Sequence Listing]
The present application contains a Sequence Listing in computer readable form, which is incorporated herein by reference.

[Reference to Deposit of Biological Material]
The present disclosure is submitted to the Agricultural Research Service Culture Collection (ARSCC), 1815 North University Street, Peoria, IL 61604, USA, Budapest for International Recognition of the Deposit of Microorganisms for the Purposes of Patent Proceedings. Contains references to biological material deposited under the provisions of the Convention.

〔background〕
Inoculum compositions containing agriculturally beneficial microorganisms are well known in the art. 5,586,411; 5,695,541; 5,804,208; 5,916,029; 6,569,425; 8,148,138; 8,278,247; 8,445,256; 8,883,679; 8,921,089; 8,999,698; Please refer to

Nonetheless, population growth and growing demand for more efficient and productive farms has led to novel formulations to prevent and treat pest infection/spread and improve crop yields. A need still exists for articles and methods.

The present disclosure provides microbial isolates useful for treating plants and plant parts, as well as compositions containing the isolates and methods of utilizing the isolates.

A first aspect of the present disclosure is Erwinia isolate (E. billingiae NRRL B-67766) deposited under Accession No. NRRL B-67766.
A second aspect of the present disclosure is a biologically pure culture of E. billingiae NRRL B-67766.
A third aspect of the present disclosure is a cell-free filtrate obtained from E. billingiae NRRL B-67766.
A fourth aspect of the present disclosure is a cell extract obtained from E. billingiae NRRL B-67766.

A fifth aspect of the present disclosure is a non-naturally occurring composition comprising E. billingiae NRRL B-67766 or a related strain thereof, a culture comprising E. billingiae NRRL B-67766 or a related strain thereof. and/or cell extracts obtained from E. billingiae NRRL B-67766 or closely related.

A sixth aspect of the present disclosure is a non-natural seed composition comprising plant propagation material, optionally seed, and a coating covering at least a portion of the outer surface of said seed, said coating comprising E. E. billingiae NRRL B-67766, biologically pure cultures of E. billingiae NRRL B-67766, cell-free filtrate obtained from E. billingiae NRRL B-67766 cultures, and/or E. billingiae NRRL B -67766 is a non-natural seed composition containing cell extracts.

A seventh aspect of the present disclosure includes E. billingiae NRRL B-67766 or its relatives, E. billingiae NRRL B-67766 or its relatives, for treating plants or plant parts Use of cell-free filtrate obtained from the culture and/or cell extract obtained from E. billingiae NRRL B-67766 or a closely related strain thereof.

An eighth aspect of the present disclosure is the use of E. billingiae NRRL B-67766 or its bacterium for killing, inhibiting the growth of, and/or inhibiting reproduction/reproduction of fungal plant pests. Use of a cell-free filtrate obtained from a culture containing a closely related strain, E. billingiae NRRL B-67766 or a closely related strain thereof, and/or a cell extract obtained from E. billingiae NRRL B-67766 or a closely related strain thereof. is.

A ninth aspect of the present disclosure is E. billingiae NRRL B-67766 or a related strain thereof, E. billingiae NRRL B-67766 or a relative thereof, for reducing disease severity in a plant or plant part. Use of cell-free filtrates obtained from cultures containing related strains, and/or cell extracts obtained from E. billingiae NRRL B-67766 or related strains thereof.

A tenth aspect of the present disclosure is E. billingiae NRRL B-67766 or a relative thereof, E. billingiae NRRL B-67766, for increasing growth and/or yield in a plant or plant part. or cell-free filtrate obtained from cultures containing strains related thereto, and/or cell extracts obtained from E. billingiae NRRL B-67766 or strains related thereto.

An eleventh aspect of the present disclosure is obtained from a culture comprising E. billingiae NRRL B-67766 or a related strain thereof, E. billingiae NRRL B-67766 or a related strain thereof, in a plant growth medium. and/or a cell extract obtained from E. billingiae NRRL B-67766 or a closely related strain thereof.

A twelfth aspect of the present disclosure is a plant propagation material, optionally a seed, containing E. billingiae NRRL B-67766 or a closely related strain thereof, E. billingiae NRRL B-67766 or a closely related strain thereof. and/or a cell extract obtained from E. billingiae NRRL B-67766 or a closely related strain thereof.

A thirteenth aspect of the present disclosure is a cell-free culture obtained from a plant comprising E. billingiae NRRL B-67766 or a related strain thereof, E. billingiae NRRL B-67766 or a related strain thereof A method comprising applying a filtrate and/or a cell extract obtained from E. billingiae NRRL B-67766 or a closely related strain thereof.

A fourteenth aspect of the present disclosure is obtained from a culture comprising E. billingiae NRRL B-67766 or a related strain thereof, E. billingiae NRRL B-67766 or a related strain thereof, in a plant growth medium. and/or a cell extract obtained from E. billingiae NRRL B-67766 or a closely related strain thereof.

A fifteenth aspect of the present disclosure comprises introducing into a plant growth medium a non-natural seed composition comprising plant propagation material, optionally seeds, and a coating covering at least a portion of the outer surface of said seeds. wherein said coating is E. billingiae NRRL B-67766, biologically pure culture of E. billingiae NRRL B-67766, cell-free filtrate obtained from E. billingiae NRRL B-67766 culture and/or a cell extract obtained from E. billingiae NRRL B-67766.

(Original text not mentioned)

[Detailed description]
This description is not intended to be a detailed listing of all of the various ways in which the invention can be implemented or of all the features that can be added to the invention. For example, features illustrated with respect to one embodiment may be incorporated into other embodiments, and features illustrated with respect to a particular embodiment may be deleted from that embodiment. Moreover, numerous modifications and additions to the various embodiments suggested herein that do not depart from the invention will be apparent to those skilled in the art in light of this disclosure. Accordingly, the following description is intended to exemplify several specific embodiments of the invention and is not exhaustive of all permutations, combinations and variations.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

Unless defined otherwise, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Moreover, terms such as those defined in common dictionaries are to be construed to have a meaning consistent with their meaning in the context of the specification and the relevant art, and are expressly defined herein. It will be understood that it should not be construed in an idealized or overly formal sense unless otherwise specified. Well-known functions or constructions may not be described in detail for brevity and/or clarity.

As used herein, the singular forms "a," "an," and "the" include plural forms unless the context clearly dictates otherwise.

As used herein, the terms "acaricide" and "acaricidal activity" mean that the application is toxic to mites (i.e., kills mites, inhibits the growth of mites and/or or control the reproduction/reproduction of mites) or a combination of agents.

As used herein, the term "agronomically beneficial agent" means any agent (e.g., chemical or biological agent) whose application causes or provides a beneficial and/or beneficial effect in agriculture. ) or a combination of agents, including, but not limited to, agriculturally beneficial microorganisms, biostimulants, nutrients, biocides (e.g., acaricides, fungicides, herbicides, insecticides, and nematicides). ) and plant signaling molecules.

As used herein, the term "agronomically beneficial microorganism" refers to at least one agriculturally beneficial property (e.g., the ability to fix nitrogen, the ability to solubilize phosphates, and/or , the ability to produce agronomically beneficial agents such as plant signaling molecules and insecticides).

As used herein, the term "agronomically acceptable carrier" means an agriculturally beneficial carrier that does not cause/result in undue adverse effects on plant growth and/or yield. Refers to a substance or composition that can be used to deliver an agent to a plant, plant part or plant growth medium (eg, soil). As used herein, the term "foliage-compatible carrier" refers to a plant or plant part that does not cause/result in undue adverse effects on the plant, plant part, plant growth, plant health, etc. refers to materials that can be foliarly applied to As used herein, the term "seed compatible carrier" refers to a material that can be applied to seeds without causing/causing undue adverse effects to plants growing from seeds, seed germination, etc. point to As used herein, the term "soil compatible carrier" refers to materials that can be added to soil without causing/causing undue detrimental effects on plant growth, soil structure, soil drainage, etc. point to

As used herein, the term "and/or" when interpreted to any and all combinations of one or more of the associated listed items, as well as the alternative ("or") is intended to include the lack of combinations. Thus, the phrase "A, B and/or C" should be interpreted as "A, A and B, A and B and C, A and C, B, B and C, or C."

As used herein, the terms "associated with," "with," and "associated with" refer to the relationship between a microbial strain or inoculum composition of the present disclosure and a plant or plant part. When used for, refers to at least juxtaposition or close proximity of the microbial strain or inoculum composition and the plant or plant part. Such juxtaposition or proximity may be achieved by contacting or applying the microbial strain or inoculum composition to the plant or plant part and/or the plant or plant part to be grown (or currently growing). It can be achieved by applying the microbial strain or inoculum composition to the plant growth medium (eg soil). According to some embodiments, the microbial strain or inoculum composition is applied to the soil at, near or around the site where the plant or plant part is to be (or is currently growing). be.

As used herein, the term "aqueous" refers to compositions that contain traces or more of water (ie, greater than 0.5% water by weight based on the total weight of the composition).

As used herein, the term "biologically pure culture" means essentially free of biological contaminants and different subcultures taken therefrom are identical or substantially Refers to microbial cultures that have genetic homogeneity, such that they exhibit generally identical genotypes and phenotypes. In some embodiments, a biologically pure culture is 100% pure (i.e., all subcultures taken therefrom are at least 90, 91, 92, 93, 94, 95, 96 , 97, 98, 99, 99.5, 99.6, 99.7, 99.8 or 99.9% pure (i.e. at least 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 99.5, 99.6, 99.7, 99.8 or 99.9% show identical genotype and phenotype).

As used herein, the term "biostimulatory agent" means that its application affects one or more metabolic and/or physiological processes (e.g. carbohydrate biosynthesis, ion uptake) in a plant or plant part. , nucleic acid uptake, nutrient delivery, photosynthesis and/or respiration).

As used herein, the term "BRADY" means "Bradyrhizobium spp. (Soybean Rhizobium)" 8A57, Bradyrhizobium elkanii SEMIA 501, Bradyrhizobium Bradyrhizobium elkanii SEMIA 587, Bradyrhizobium elkanii SEMIA 5019, Bradyrhizobium japonicum 61A227, Bradyrhizobium japonicum 61A228, Bradyrhizobium japonicum 61A228 Bradyrhizobium japonicum 61A273, Bradyrhizobium japonicum E-109, Bradyrhizobium japonicum NRRL B-50586 (also deposited as NRRL B-59565) was deposited), Bradyrhizobium japonicum NRRL B-50587 (also deposited as NRRL B-59566), Bradyrhizobium japonicum NRRL B-50588 (NRRL B-59566) 59567), Bradyrhizobium japonicum NRRL B-50589 (also deposited as NRRL B-59568), Bradyrhizobium japonicum NRRL B-50590 (also deposited as NRRL B-59569), Bradyrhizobium japonicum NRRL B-50591 (also deposited as NRRL B-59570), Bradyrhizobium japonicum NRRL B-50592 (also deposited as NRRL B-59571), Bradyrhizobium japonicum japonicum) NRRL B-50593 (also deposited as NRRL B-59572), Bradyrhizobium japonicum NRRL B-50594 (also deposited as NRRL B-50493), Bradyrhizobium japonicum Bradyrhizobium japonicum NRRL B-50608, Bradyrhizobium japonicum NRRL B-50609, Bradyrhizobium japonicum NRRL B-50610, Bradyrhizobium japonicum (Bradyrhizobium japonicum) NRRL B-50611, Bradyrhizobium japonicum NRRL B-50612, Bradyrhizobium japonicum NRRL B-50726, Bradyrhizobium japonicum japonicum) NRRL B-50727, Bradyrhizobium japonicum NRRL B-50728, Bradyrhizobium japonicum NRRL B-50729, Bradyrhizobium japonicum NRRL B-50730, Bradyrhizobium japonicum SEMIA 566, Bradyrhizobium japonicum SEMIA 5079, Bradyrhizobium japonicum SEMIA 5080, Bradyrhizobium Bradyrhizobium japonicum USDA 6, Bradyrhizobium japonicum USDA 110, Bradyrhizobium japonicum USDA 122, Bradyrhizobium japonicum Bradyrhizobium japonicum USDA 123, Bradyrhizobium japonicum USDA 127, Bradyrhizobium japonicum USDA 129 and/or Bradyrhizobium japonicum USDA should be construed as a shorthand substitute for "532C".

As used herein, the term "cell-free filtrate" refers to the filtrate of a bacterial culture devoid or substantially devoid of viable bacterial cells.

As used herein, the term "cell extract" refers to a substance or combination of substances that is isolated from bacterial cells.

As used herein, the terms "colony forming unit" and "cfu" refer to a suitable cell when conditions (e.g., temperature, moisture, nutrient availability, pH, etc.) are favorable for germination and/or microbial growth. Refers to microbial cells/spores capable of growing on or in a growth medium or growth environment (eg, soil).

As used herein, the term "consisting essentially of" when used in reference to inoculation compositions and methods of the present disclosure, where additional components/steps make up the compositions/methods It is meant that the compositions/methods may contain additional components/steps so long as they are not substantially changed. The term "substantially alter" when used with respect to compositions/methods of the present disclosure refers to an increase or decrease in the effectiveness of the composition/method by at least 20%. For example, a component added to an inoculum composition of the present disclosure will increase or decrease the composition's ability to reduce one or more aspects of disease severity by at least 20%. can be regarded as "substantially changing."

As used herein, the term "diazotroph" refers to the conversion of atmospheric nitrogen ( _N2 ) into a form available by plants or plant parts (e.g., ammonia ( _NH3 ), ammonium ₍ NH4)). ⁺ ), etc.).

As used herein, the term "dispersant" means that the application of which reduces particle cohesion, the surface tension of a liquid, the interfacial tension between two liquids and/or the interfacial tension between a liquid and a solid. Refers to a drug or combination of drugs that causes

As used herein, the terms "effective amount," "effective concentration," and "effective amount/concentration" (and their grammatical conjugations) refer to desired effects (e.g., reduced disease severity and / or an amount or concentration sufficient to cause an improved crop yield). The absolute amount/concentration sufficient to cause the desired effect depends on the type and extent of the desired effect, the type, size and volume of seed to which the inoculum composition is to be applied, the stability of the microorganisms in the inoculum composition. , as well as storage conditions (eg, temperature, relative humidity, duration). Those of ordinary skill in the art would understand how to select effective amounts/concentrations using routine dose-response studies.

As used herein, the term "endogenous gene" refers to a genetic gene consisting of endogenous polynucleotides.

As used herein, the term "endogenous polynucleotide" refers to a polynucleotide native to the host cell to which it is being referred.

As used herein, the term "enhanced dispersion" refers to one or more controls (e.g., one or more of the components found in the inoculant composition of the present disclosure that lacks Refers to an improvement in one or more characteristics of a microbial dispersion when compared to a control composition that is identical to the inoculum composition of the present disclosure, except that the composition is identical to the inoculum composition of the present disclosure. Exemplary microbial dispersal characteristics include, but are not limited to, the percentage of microorganisms present as single cells/single spores when the inoculum composition is diluted with water. contained therein when compared to a control composition (e.g., a control composition that is identical to the inoculation composition except that it lacks one or more of the components found in the inoculation composition) An inoculum composition that improves one or more of the microbial dispersal characteristics of the microorganisms that are used provides improved dispersal and can be referred to as an "easily dispersible inoculum composition."

As used herein, the terms "enhanced growth" and "enhanced plant growth" refer to growth compared to one or more control plants (e.g., plants sprouted from untreated seeds or untreated plants). refers to an improvement in one or more characteristics of plant growth and/or growth. Exemplary plant growth/growth characteristics include, but are not limited to, biomass, carbohydrate biosynthesis, chlorophyll content, cold tolerance, drought tolerance, height, leaf length, leaf mass, leaf number, leaf surface area. , leaf volume, lodging resistance, nutrient uptake and/or accumulation (e.g., of ammonium, boron, calcium, copper, iron, magnesium, manganese, nitrate, nitrogen, phosphate, phosphorus, potassium, sodium, sulfur and/or zinc). uptake/accumulation), photosynthetic rate, root area, root diameter, root length, root mass, nodule formation (e.g., nodule mass, nodule number, nodule volume), root number, root surface area, root volume, salt tolerance, seed germination , seedling formation, shoot diameter, shoot length, shoot mass, number of shoots, shoot surface area, shoot volume, spread, colony, stomatal conductance and survival. Unless otherwise indicated, references to enhanced plant growth shall mean that the compositions and methods of the present disclosure promote plant growth by enhancing nutrient availability, improving soil properties, and the like. should not be construed as suggesting that the compositions and methods of the disclosure act as plant growth regulators.

As used herein, the term "enhanced stability" refers to the absence of one or more controls (e.g., one or more of the components found in the inoculum compositions of the present disclosure). Refers to an improvement in one or more characteristics of microbial stability when compared to a control composition that is identical to the inoculum composition of the present disclosure, except for the control composition. Exemplary microbial stability properties include, but are not limited to, the ability to germinate and reproduce after being applied on seeds and/or after being stored for a period of time; and/or the ability to cause a desired effect (e.g., improved plant yield and/or increased pesticide activity (pesticidal activity) after and/or after storage for a predetermined period of time, each under the same conditions ( Microorganisms exhibiting improved one or more microbial stability characteristics compared to control microorganisms when subjected to seed dressing and storage conditions, e.g., seed dressing and storage conditions, are referred to as "stable microorganisms compared to a control composition (e.g., a control composition that is identical to the inoculation composition except that it lacks one or more of the components found in the inoculation composition) If so, an inoculum composition that improves one or more microbial stability characteristics of the microorganisms contained in the composition provides enhanced stability and can be referred to as a "stable inoculum composition." .

As used herein, the terms "enhanced viability" and "enhanced microbial viability" refer to a control composition (e.g., one of the components found in the inoculum composition of the present disclosure) Survival of one or more microorganisms in an inoculum composition as compared to one or more microorganisms in a control composition, which is identical to the inoculum composition of the present disclosure except lacking refers to the improvement of in the composition when compared to a control composition (e.g., a control composition that is identical to the inoculation composition except that it lacks one or more of the components found in the inoculation composition) An inoculum composition that improves the viability of one or more of the microorganisms contained in a can be referred to as a stable inoculum composition because it provides improved viability.

As used herein, the terms "enhanced yield" and "enhanced plant yield" are used when compared to one or more control plants (e.g., control plants sprouted from untreated seeds). , refers to an improvement in one or more characteristics of plant yield. Exemplary plant yield characteristics include, but are not limited to, biomass; bushels per acre (1 bushel = approximately 27.2 kg); grain weight per plot ( ^GWTPP ); percentage of plants in a given area (e.g., plot) that cannot produce grain; yield at standard moisture (YSMP), e.g., grain yield at standard moisture (GYSMP); plot yield per plot (YPP), eg, grain weight per plot (GWTPP); and yield reduction (YRED). Unless otherwise indicated, references to enhanced plant yield mean that the compositions and methods of the present disclosure can increase plant yield by increasing nutrient availability, improving soil properties, and the like. should not be construed as suggesting that the compositions and methods of the present disclosure act as plant growth regulators.

As used herein, the term "expression" includes any process involved in the production of a polypeptide including, but not limited to, transcription, post-transcriptional modification, translation, translational modification and secretion. Expression can be measured, for example, by techniques well known in the art, and increased expression can be detected, for example, by measuring levels of mRNA and/or levels of translated polypeptide.

As used herein, the term "foliage" includes, but is not limited to, leaves, stalks, stems, flowers, fruiting bodies and fruits that usually grow on the ground. Refers to plant parts.

As used herein, the terms "foliar application", "foliarly applied" and grammatical conjugations thereof refer to the application of one or more active ingredients to the foliage of a plant (e.g. leaves of a plant). Refers to dissemination. Application may be by any suitable means including, but not limited to, spraying the plants with a composition containing the active ingredient. In some embodiments, the active ingredient is applied to the leaves, stems and/or stalks of the plant and not to the flower, fruiting body or fruit of the plant.

As used herein, the terms "fungicide" and "fungicidal" refer to agents whose application is toxic to fungi (i.e., kills fungi, inhibits fungal growth, and/or to inhibit fungal reproduction/proliferation) or combination of agents.

As used herein, the term "fulvic acid" includes pure fulvic acid and fulvate. Non-limiting examples of fulvic acids include ammonium fulvicate, boron fulvicate, potassium fulvicate, sodium fulvicate, and the like. In some embodiments, the fulvic acid comprises, consists essentially of, or consists of MDL No. MFCD09838488 (CAS No. 479-66-3).

As used herein, the terms "herbicide" and "herbicidal" mean that the application is toxic to weeds (i.e., kills weeds, inhibits weed growth, and/or kills weeds). refers to a reproductive/reproduction-inhibiting) agent or combination of agents.

As used herein, the term "heterologous gene" refers to a gene comprising a heterologous polypeptide.

As used herein, the term "heterologous polynucleotide" refers to a polynucleotide that is not native to the host cell to which it is referred. For the purposes of this disclosure, an exogenous copy of a polynucleotide that is otherwise native to the host cell to which it is referred is considered a heterologous polynucleotide.

As used herein, the term "humic acid" includes pure humic acid and humates. Non-limiting examples of humic acids include ammonium humate, boron humate, potassium humate, sodium humate, and the like. In some embodiments, the humic acid is MDL No. MFCD00147177 (CAS No. 1415-93-6), MDL No. MFCD00135560 (CAS No. 68131-04-4), MDL No. MFCS22495372 (CAS No. 68514-28-3), comprising, consisting essentially of, or consisting of one or more of CAS No. 93924-35-7 and CAS No. 308067-45-0.

The terms “inoculum composition” and “inoculum” refer to a composition comprising microbial cells and/or spores, wherein the conditions (e.g., temperature, humidity, nutrient availability, pH, etc.) are It can be grown on or in the growing environment (eg, soil) when it is favorable for growth.

As used herein, the terms "insecticide" and "insecticidal" mean that the application is toxic to insects (i.e., kills insects, inhibits insect growth, inhibits insect reproduction). /reproducing) refers to an agent or combination of agents.

As used herein, the term "isolated microbial strain" refers to a microorganism that has been separated from the environment in which it is normally found.

As used herein, the term "isomer" refers to enantiomers and diastereomers, and all conformers, rotamers and tautomers, unless otherwise indicated. includes all stereoisomers, including, but not limited to, the compound and/or molecule to which it refers. The compounds and/or molecules disclosed herein may be in either substantially pure levorotatory or dextrorotatory form, or in racemic mixtures, or as enantiomers in any ratio, in all Enantiomers are included. Where an embodiment discloses the (D)-enantiomer, the embodiment also includes the (L)-enantiomer; where an embodiment discloses the (L)-enantiomer, the practice of Forms also include the (D)-enantiomer. When an embodiment discloses the (+)-enantiomer, the embodiment also includes the (-)-enantiomer; when an embodiment discloses the (-)-enantiomer, the practice Forms also include (+)-enantiomers. Where an embodiment discloses the (S)-enantiomer, the embodiment also includes the (R)-enantiomer; where an embodiment discloses the (R)-enantiomer, the practice of Forms also include the (S)-enantiomer. Embodiments are intended to include any diastereomers of the compounds and/or molecules referred to herein in diastereomerically pure form and in mixtures in all ratios. Unless stereochemistry is explicitly indicated in a chemical structure or chemical name, a chemical structure or chemical name refers to all possible stereoisomers, conformers, rotamers and tautomers of the indicated compound and/or molecule. It is intended to encompass the body.

As used herein, the term "modified microbial strain" refers to a microbial strain that has been modified from a strain isolated from nature. Modified microbial strains include, but are not limited to, chemical or other forms of induced mutations to polynucleotides within any genome within the strain; insertions or deletions of one or more nucleotides within any genome within the strain. inversion of at least one segment of DNA within any genome within the strain; transposition of any genome within the strain; homozygous or heterozygous polynucleotide segments to any genome within the strain. introduction of one or more phages into any genome of the strain; transformation of any strain resulting in the introduction of stably replicating autonomous extrachromosomal DNA into the strain; any alteration to the genomic or total DNA composition within a strain isolated from nature as a result of binding with a different microbial strain of obtain. The term modified microbial strain includes (a) one or more heterologous nucleotide sequences, (b) one or more non-naturally occurring copies of a nucleotide sequence isolated from nature (i.e., naturally occurring in the microbial strain from which the modified microbial strain is derived). (c) lack of one or more nucleotide sequences that would otherwise be present in the native reference strain, e.g., by deleting nucleotide sequences; and (d) added strains with extrachromosomal DNA. In certain embodiments, the modified microbial strain comprises a combination of two or more nucleotide sequences (e.g., two or more native genes not naturally occurring in the same microbial strain) or located at different loci than the native loci. Includes nucleotide sequences isolated from nature.

As used herein, the terms "nematicidal" and "nematicidal" mean that the application is toxic to nematodes (i.e., kills nematodes, nematode growth agents). and/or inhibit nematode reproduction/proliferation) or a combination of agents.

As used herein, the term “nitrogen-fixing organisms” refers to atmospheric nitrogen (N ₂ ) in a form available by plants or plant parts (e.g. ammonia (NH ₃ ), ammonium (NH ₄ ⁺ )). etc.).

As used herein, the term "non-aqueous" refers to compositions containing no more than trace amounts of water (ie, no more than 0.5% by weight of water, based on the total weight of the composition).

As used herein, the term "nutrient" refers to a compound or element useful in providing nutrients to a plant (e.g., vitamins, macrominerals, trace elements necessary for plant growth and/or viability). nutrients, trace elements, organic acids, etc.).

As used herein, the term "PENI" refers to the blue mold "Penicillium bilaiae ATCC 18309, Penicillium bilaiae ATCC 20851, Penicillium bilaiae ATCC 22348, Penicillium bilaiae・Penicillium bilaiae NRRL 50162, Penicillium bilaiae NRRL 50169, Penicillium bilaiae NRRL 50776, Penicillium bilaiae NRRL 50777, Penicillium bilaiae NRRL 507, Penicillium bilaiae NRRL 507, Penicillium bilaiae NRRL 50169 Penicillium bilaiae NRRL 50777, Penicillium bilaiae NRRL 50778, Penicillium bilaiae NRRL 50779, Penicillium bilaiae NRRL 50780, Penicillium bilaiae NRRL1, Penicillium bilaiae NRRL 507 Penicillium bilaiae NRRL 50782, Penicillium bilaiae NRRL 50783, Penicillium bilaiae NRRL 50784, Penicillium bilaiae NRRL 50785, Penicillium bilaiae NRRL6, Penicillium bilaiae NRRL6, Penicillium bilaiae NRRL 5078 Penicillium bilaiae NRRL 50787, Penicillium bilaiae NRRL 50788, Penicillium bilaiae NRRL 67154, Penicillium bilaiae NRRL 67155, Penicilliu m bilaiae) NRRL 67156, Penicillium bilaiae NRRL 67157, Penicillium bilaiae NRRL 67158, Penicillium bilaiae NRRL 67159, Penicillium bilaiae RS7B-SD1, Penicillium bilaiae Compactum (Penicillium brevicompactum) AgRF18, Penicillium canescens ATCC 10419, Penicillium expansum ATCC 24692, Penicillium expansum YT02, Penicillium fellicillium ripanisatanum ATCC 48694 (Penicillium gaestrivorus) NRRL 50170, Penicillium glabrum DAOM 239074, Penicillium glabrum CBS 229.28, Penicillium janthinellum ATCC 10455, Penicillium janthinellum ATCC 10455, Penicillium lanosocoeruleum 19, ATCC 19 AT Penicillium radicum ATCC 201836, Penicillium radicum FRR 4717, Penicillium radicum FRR 4719, Penicillium radicum N93/47267 and/or Penicillium raistrickii should be construed as a shorthand substitute for "ATCC 10490".

As used herein, "Penicillium bilaiae" includes all iterations of species names such as "Penicillium bilai" and "Penicillium bilaii". ) is intended to include

As used herein, the terms "% identity", "% identity" and "% identity" refer to the closeness (relatedness) of two or more nucleotide or amino acid sequences, which (i) compares two optimally aligned sequences within a comparison window, and (ii) identifies identical nucleobases (for nucleotide sequences) or amino acid residues (for proteins) in both sequences. Determine the number of positions present, determine the number of matched positions, (iii) divide the number of matched positions by the total number of positions in the comparison window, and (iv) multiply the quotient by 100%. It can be calculated by determining the percent identity. If "% identity" is calculated relative to a reference sequence, % identity is determined by the number of matched positions over the entire region of the alignment divided by the total length of the reference sequence. Thus, for the purposes of the present invention, the "% identity" of a query sequence is the value between the two sequences when the two sequences (query and subject) are optimally aligned (allowing for gaps in their alignment). Equals the number of identical positions divided by the total number of positions in the query sequence over that length (or comparison window), then multiplied by 100%.

As used herein, the term "pest" refers to organisms and viruses that spread disease, damage host plants, and/or compete for soil nutrients. It includes any organism or virus that has an adverse effect. The term "pest" includes organisms or viruses that are associated with plants and are known to cause adverse effects on plant health and/or vigor. Plant pests include, but are not limited to, arachnids (e.g., mites, ticks, spiders, etc.), bacteria, fungi, gastropods (e.g., slugs, snails, etc.), invasive plants (e.g., weeds), insects (e.g., whiteflies, thrips, weevils, etc.), nematodes (e.g., root-knot nematodes, soybean cyst nematodes, etc.), rodents and viruses [e.g., tobacco mosaic virus (TMV), tomato spotted wilt virus (TSWV), cauliflower mosaic virus (CaMV ) etc.].

As used herein, the terms "pesticide" and "pesticidal activity" mean that the application is toxic to pests (i.e., kills pests, kills pests, refers to an agent or combination of agents that inhibits growth and/or inhibits pest reproduction). Non-limiting examples of pesticides include acaricides, fungicides, herbicides, insecticides, nematicides, and the like.

As used herein, the term "phosphate-solubilizing microorganism" refers to a microorganism capable of converting insoluble phosphate into a soluble form of phosphate.

As used herein, the term "plant" includes all plant groups including, but not limited to, agricultural, horticultural and forestry plants. The term "plant" refers to plants obtained by conventional plant breeding and optimization methods (e.g., marker-assisted selection (MAS; DNA marker-assisted selection)) and protectable or protectable by the rights of plant breeders. It includes plants obtained by genetic engineering, including cultivars that are not genetically modified.

As used herein, the term "plant cell" refers to a cell of a plant, a cell harvested from a plant, or a cell derived from a cell harvested from a plant. The term "plant cell" thus includes cells within seeds, suspension cultures, embryos, meristematic regions, callus tissue, leaves, shoots, gametophytes, sporophytes, pollen and microspores.

As used herein, the term "plant growth regulator" means that the application of which accelerates or retards the growth/maturation rate of plants through a direct physiological effect on the plant or Refers to an agent or combination of agents that otherwise alters plant behavior through physical physiological effects. “Plant growth regulator” is exempt from the definition of “plant regulator” as set forth in Chapter II(v) of the Insecticides, Fungicides and Rodenticides Act (FIFRA) (7 U.S.C. §136(v)). should not be construed to include any drug or combination of drugs. Thus, "plant growth regulators" include microorganisms applied to plants, plant parts or plant growth media for the purpose of enhancing the availability and/or uptake of nutrients, nutrients required for normal plant growth, does not include soil amendments applied for the purpose of improving soil properties favorable to soil or vitamin hormone preparations as defined by 40 C.F.R. § 152.6(f).

As used herein, the term "plant part" refers to any part of a plant, including cells and tissues derived from the plant. Thus, the term "plant part" can refer to any component or organ of a plant (eg, leaves, stems, roots, etc.), plant tissue, plant cells and seeds. Examples of plant parts include, but are not limited to, anthers, embryos, flowers, fruits, fruiting bodies, leaves, ovules, pollen, rhizomes, roots, seeds, shoots, stems and tubers, as well as shoots, rootstocks, protoplasts, callus, and the like. are mentioned.

As used herein, the term "plant propagation material" refers to a plant part from which a complete plant can be produced. Examples of plant propagation material include, but are not limited to, seedlings (eg, leaves, stems), rhizomes, seeds, tubers and cells/tissues that can be cultured in whole plants.

As used herein, the term "descendants" includes both the direct offspring and any distant descendants thereof. Progeny can be produced using any suitable method including, but not limited to, protoplast fusion, traditional breeding programs and combinations thereof.

As used herein, the term "reduced disease severity" refers to a reduction in disease severity when compared to one or more controls (e.g., control plants sprouted from untreated seeds, untreated control plants, etc.). Refers to a reduction in one or more characteristics of degree. Exemplary disease severity characteristics include, but are not limited to, disease incidence (i.e., percentage of infected/spread diseased plants within a particular area/location), diseased area (e.g., surface area exhibiting lesions), Pathogen load (i.e. amount/concentration of pests per acre per plant), rate of infection/spread, symptom severity (e.g. yellowing, necrosis, etc.) and toxin load ( For example, amount/concentration of pest toxin per acre per plant). Unless otherwise indicated, references to reduced disease severity do not mean that the microbial strains, cultures, acellular filtrates, cell extracts, inoculum compositions and methods of the present disclosure kill pests, thereby reducing pest severity. By inhibiting growth, by inhibiting reproduction/reproduction of pests, etc., should be construed to mean reducing disease severity, and the microbial strains, cell-free filtrates, cell extracts of the present disclosure. should not be construed as suggesting that the products, inoculum compositions and methods act as plant growth regulators.

As used herein, the terms "spore" and "microbial spore" refer to a microorganism in its dormant, protective phase.

As used herein, the term "stabilizing compound" refers to an agent or combination of agents that promotes the viability and/or stability of microorganisms in an inoculum composition.

As used herein with respect to an inoculum composition, the term "stable" refers to an inoculum composition in which microorganisms exhibit improved stability and/or improved viability. In general, an inoculum composition may be designated as "stable" if it improves the viability and/or at least one microbial stability characteristic of at least one microorganism contained therein.

As used herein with respect to microbial spores, the term "viability" means viability over a given period of time [i.e., conditions (e.g., temperature, moisture, nutrient availability, pH, etc.) Where preferred, it refers to the proportion (percentage) of microbial spores capable of growing on or in the growing environment (eg, on the seed and/or in the soil).

As used herein, the term "toxin" refers to the extractable, agrochemically active (pesticidal) component of a microorganism.

As used herein, the term "whole broth culture" refers to a population of microorganisms with the liquid medium in which it was grown/propagated.

Although several aspects of the disclosure will be described hereinafter with respect to embodiments thereof, various changes in form and detail may be made without departing from the spirit and scope of the disclosure as defined by the claims. will be understood by those skilled in the art.

All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety to the extent that they do not contradict the disclosure explicitly set forth herein.

The present disclosure provides strains, cultures, cell-free filtrates, cell extracts (eg, toxins) and inoculum compositions useful for preventing and/or treating myriad plant infections/spreads.

In some embodiments, the strain is an agrochemically active (pesticidal) Erwinia strain, such as the isolated Erwinia billingiae strain (E. billingiae NRRL B-67766) having accession number NRRL B-67766. is a pesticidal Erwinia villinghier strain.

In some embodiments, the strain is a progeny of E. billingiae NRRL B-67766, a modified microbial strain derived from E. billingiae NRRL B-67766, or a progeny of E. Billingiae NRRL B-67766. is a modified microbial strain obtained from

In some embodiments, the strain is about/at least relative to SEQ ID NO:1 , 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.8, 96.85 , 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 86.8 , 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.58, 99.58 , 99.91, 99.92, 99.93, 99.94, 99.95, 99.96, 99.97, 99.98, 99.99 or 100% identical 16S sequences.

In some embodiments, the strain is about/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9 relative to that of E. billigiae NRRL B-67766 , 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.5, 97 , 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.85, 98.75 , 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9, 99.91, 99.93, 99.92 , 99.95, 99.96, 99.97, 99.98, 99.99 or 100% identical whole genome sequences.

The present disclosure includes closely related strains of E. billingiae NRRL B-67766 that exhibit agrochemical (pesticidal) activity, including, but not limited to, closely related progeny of E. billingiae NRRL B-67766 (e.g., , about/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4 relative to SEQ ID NO: 1, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.0 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 9.3, 99.25, 99.25 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9, 99.91, 99.92, 99.93, 99.94, 99.95, 99.96, 99.97, 99.98, 99.99 or 100% identical 16S sequences and/or About/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25 relative to the entire genome sequence of E. billigiae NRRL B-67766 , 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.9, 97.85 , 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85 , 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9 or 99.95% identical whole genome progeny with sequence), closely related modified microbial strains derived from E. billingiae NRRL B-67766 (e.g., derived from E. billingiae NRRL B-67766 and about/at least 95, 95.5, 95.55 relative to SEQ ID NO:1) , 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.78, 96.57 , 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.5, 98.4, 98.4 , 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.59, 99.75, 99.65 , 99.8, 99.85, 99.9, 99.91, 99.92, 99.93, 99.94, 99.95, 99.96, 99.97, 99.98, 99.99 or about / at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 9 7.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 98.95, 98.95 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9 or 99.95% identical modified microbial strains), Related modified microbial strains derived from progeny of E. billingiae NRRL B-67766 (e.g., derived from one or more progeny of E. billingiae NRRL B-67766 and about/at least 95, 95.5, relative to SEQ ID NO: 1) 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 65, 96.65, 96.6, 96.65 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.35, 98.4 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.6, 99.6 99.75, 99.8, 99.85, 99.9, 99.91, 99.92, 99.93, 99.94, 99.95, 99.96, 99.97, 99.98, 99.99 or to a 16S sequence that is 100% identical and/or to the whole genome sequence of E. billigiae NRRL B-67766 about/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96 .2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 987.8 , 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.99 , 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9 or 99.95% identical whole genome sequences), and Other closely related strains (e.g., about/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25 relative to SEQ ID NO:1) , 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.9, 97.85 , 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.1, 99 or and/or about/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7 for the 16S sequence and/or the whole genome sequence of E. billigiae NRRL B-67766 , 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.8, 96.85 , 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 86.8 , 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.58, 99.58 or agrochemically active Erwinia strains with whole genome sequences that are 99.95% identical.

In some embodiments, the strain is a pesticidally active Pseudomonas strain, such as the Pseudomonas gessardii isolate having Deposit Accession Number (Accession Number) NRRL B-67767. • A pesticidal active Pseudomonas libanesis, such as the P. gessardii NRRL B-67767 strain or the Pseudomonas libanesis isolate having the Deposit Accession Number (Accession Number) NRRL B-67769.

In some embodiments, the strain is a descendant of P. gessardi NRRL B-67767, a modified microbial strain derived from P. gessardi NRRL B-67767, or P. gessardii NRRL B-67767 is a modified microbial strain derived from the progeny of

In some embodiments, the strain is about/at least 95,95.05,95.1,95.15,95.2,95,25,95.3,95.35,95.4,95.45,95.5,95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.87, 96.7, 96.7 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.5, 98.45, 98.45 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.5, 99.7, 99.7 99.85, 99.9, 99.91, 99.92, 99.93, 99.94, 99.95, 99.96, 99.97, 99.98, 99.99 or 16S sequences that are 100% identical.

In some embodiments, the strain is about/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9 relative to that of P. gessardii NRRL B-67767 , 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.5, 97 , 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.85, 98.75 , 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9, 99.91, 99.93, 99.92 , 99.95, 99.96, 99.97, 99.98, 99.99 or 100% identical whole genome sequences.

The present disclosure provides related strains of P. gessardii NRRL B-67767 that exhibit pesticidal activity, including, but not limited to, related progeny of P. gessardii NRRL B-67767 (e.g., relative to SEQ ID NO:2). about/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.65, 96.65 , 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.2, 98.2 , 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.55, 99.45 , 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9, 99.91, 99.92, 99.93, 99.94, 99.95, 99.96, 99.97, 99.98, 99.99 or 100% identical 16S sequences and/or P. gessardii NRRL B-67767 about/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, for the whole genome sequence of 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.0 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9, 99.91, 99.92, 99.93, 99.94 progeny with whole genome sequences that are 99.97, 99.98, 99.99 or 100% identical), closely related modified microbial strains derived from P. gessardii NRRL B-67767 (e.g., derived from P. gessardii NRRL B-67767 and sequences about/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45 for number 2, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.05, 98.51 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.3, 99.3 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9, 99.91, 99.92, 99.93, 99.94, 99.95, 99.96, 99.97, 99.98, 99.99 or 100% identical 16S sequences and/or P. gessardii NRRL B-67767 about/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3 , 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97 , 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.56, 98.56 , 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.8, 99.85 , 99.92, 99.93, 99.94, 99.95, 99.96, 99.97, 99.98, 99.99 or 99.98, 99.99 or 100% identical whole genome sequences), closely related modified microorganisms derived from progeny of P. gessardii NRRL B-67767 strains (e.g., 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, derived from one or more progeny of P. gessardii NRRL B-67767 and relative to SEQ ID NO:2); 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.5, 97.6, 97.6, 97.6 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 9, 95, 98.85, 98.85 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9, 99.91, 99.92, 99.93, 99.94 99.97, 99.98, 99.99 or 100% identical to 16S sequences and/or the whole genome sequence of P. gessardii NRRL B-6776795, 9 5.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.5, 96.6, 96.6, 96 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.5, 98.3, 98.4 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 9.65, 9.65, 99.55 Modified microbial strains with whole genome sequences that are 99.7, 99.75, 99.8, 99.85, 99.9, 99.95% identical), and other closely related strains (e.g., about/at least 95, 95.5, 95.55, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.87, 96.7, 96.7 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.4, 98.4 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 95, 99.7, 99.65 99.8, 99.85, 99.9, 99.91, 99.92, 99.93, 99.94, 99.95, 99.96, 99.97, 99.98, 99.99 or 100% identical 16S sequences and/or is about/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2 relative to the entire genome sequence of P. gessardii NRRL B-67767, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.8, 97.8 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 91.05, 99, 91.05, 99 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9, 99.95% identical pesticidal Pseudomonas strains).

In some embodiments, the strain is a progeny of P. libanesis NRRL B-67769, a modified microbial strain derived from P. libanesis NRRL B-67769, or a progeny of P. libanesis NRRL B-67769. It is a derived modified microbial strain.

In some embodiments, the strain is about/at least 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.8, 96.75 96.9, 96.95, 97, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.5, 98.5, 98.5 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 9.85, 99.75, 99.75 99.9, 99.91, 99.92, 99.93, 99.94, 99.95, 99.96, 99.97, 99.98, 99.99 or 16S sequences that are 100% identical.

In some embodiments, the strain is about/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9 relative to that of P. libanesis NRRL B-67769 , 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.5, 97 , 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.85, 98.75 , 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9, 99.91, 99.93, 99.92 , 99.95, 99.96, 99.97, 99.98, 99.99 or 100% identical whole genome sequences.

The present disclosure provides related strains of P. libanesis NRRL B-67769 that exhibit agrochemical (pesticidal) activity, including, but not limited to, related progeny of P. libanesis NRRL B-67769 (e.g., about/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45 relative to SEQ ID NO:3 , 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 91.1, 9, 88 , 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.45, 99.35, 99.35 , 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9, 99.91, 99.92, 99.93, 99.94, 99.95, 99.96, 99.97, 99.98, 99.99 or 100% identical 16S sequences and/or P about/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25 for the whole genome sequence of libanesis NRRL B-67769; 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.85, 97.85, 97.85 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98. 9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9 or a whole genome sequence that is 100% identical ), closely related modified microbial strains derived from P. libanesis NRRL B-67769 (e.g., derived from P. libanesis NRRL B-67769 and about/at least 95, 95.5, 95.55 relative to SEQ ID NO:3, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.87, 96.7, 96.7 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.4, 98.4 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 95, 99.7, 99.65 99.8,99.85,99.9,99.91,99.92,99.93,99.94,99.95,99.96,99.97,99.98,99.99 or about/ At least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.55, 96.55 , 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97 9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.15 P closely related modified microbial strains derived from progeny of P. libanesis NRRL B-67769 (e.g., derived from one or more progeny of P. libanesis NRRL B-67769 and about/at least 95, 95.5, 95.55 relative to SEQ ID NO:3); , 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.78, 96.57 , 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.5, 98.4, 98.4 , 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.59, 99.75, 99.65 , 99.8, 99.85, 99.9, 99.91, 99.92, 99.93, 99.94, 99.95, 99.96, 99.97, 99.98, 99.99 or about / at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.85, 97.85, 97.85 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.5, 99.1, 99.1, 99.2 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9 or 99.95% identical modified microbial strains), and other closely related strains (e.g., about/at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35 for SEQ ID NO:3, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 958 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.32, 99.2, 99.2 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9, 99.91, 99.92, 99.93, 99.94, 99.95, 99.96, 99.97, 99.98, 99.99 or 100% sequence identity and / or about / at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95 relative to the whole genome sequence of P. libanesis NRRL B-67769 .8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 97.96 , 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.56, 98.56 or pesticide-active Pseudomonas strains with whole genome sequences that are % identical).

Strains of the present disclosure can be cultured using any suitable method, including but not limited to liquid phase fermentation and solid phase fermentation. See generally, Cunningham et al., CAN. J. BOT. 68:2270 (1990); Friesen et al., APPL. MICROBIOL. BIOTECH. 68: 397 (2005).
Strains of the present disclosure can be harvested during any suitable growing season. In some embodiments, strains of the present disclosure are allowed to reach stationary growth phase and then harvested.

Strains of the present disclosure may be subjected to, but not limited to, centrifugation (e.g. density gradient centrifugation, disc stack centrifugation, tubular circular centrifugation), coagulation, decantation, felt bed collection, filtration (e.g. drum filtration, Any suitable method can be used to harvest and/or concentrate, including sieving, ultrafiltration), flocculation, impingement and trapping (eg, cyclone spore collection, liquid impingement).

The present disclosure extends to cultures (eg, whole broth cultures) comprising, consisting essentially of, or consisting of one or more strains of the present disclosure. In some embodiments, at least 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, 95.95, 96, 96.05, 96.1, 96.15, 96.2 of subcultures taken from the culture, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.55, 97.6, 97.65, 97.7, 97.75, 97.8, 97.8, 97.8 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.75, 98.8, 98.85, 98.9, 98.95, 99, 91.05, 99, 91.05, 99 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 99.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9, 99.91, 99.92, 99.93, 99.94, 99.95, 99.96, 90.99% or 99.98, 99.97% 95, 95.5, 95.55, 95.6, 95.65, 95.7, 95.75, 95.8, 95.85, 95.9, against those of E. billigiae NRRL B-67766, P. gessardii NRRL B-67767 or P. libanesis NRRL B-67769, 95.95, 96, 96.05, 96.1, 96.15, 96.2, 96.25, 96.3, 96.35, 96.4, 96.45, 96.5, 96.55, 96.6, 96.65, 96.7, 96.75, 96.8, 96.85, 96.9, 96.95, 97, 97.5, 97.5, 97.5 97.65, 97.7, 97.75, 97.8, 97.85, 97.9, 97.95, 98, 98.05, 98.1, 98.15, 98.2, 98.25, 98.3, 98.35, 98.4, 98.45, 98.5, 98.55, 98.6, 98.65, 98.7, 98.8, 98.75 98.9, 98.95, 99, 99.05, 99.1, 99.15, 99.2, 99.25, 99.3, 99.35, 99.4, 99.45, 9 9.5, 99.55, 99.6, 99.65, 99.7, 99.75, 99.8, 99.85, 99.9, 99.91, 99.92, 99.93, 99.94, 99.95, 99.96, 99.97, 99.98, 99.99 or 100% identical genotypes. In some embodiments, the culture is a biologically pure culture of E. billigiae NRRL B-67766, P. gessardii NRRL B-67767 or P. libanesis NRRL B-67769.

The disclosure also extends to cell-free filtrates obtained from cultures of the disclosure, and cell extracts obtained from strains of the disclosure. Cell-free filtrates and cell extracts can be prepared by any suitable means known in the art, including, but not limited to, enzyme-mediated lysis, osmotic lysis, trituration and sonication. See, eg, PROTEIN PURIFICATION PROTOCOLS, Doonan (ed.), Humana Press (1996).

The strains, cultures, cell-free filtrates and cell extracts of the present disclosure can be formulated into any suitable type of composition including, but not limited to, foliar treatments, seed dressings and soil treatments. .

In some embodiments, the disclosure provides inoculum compositions comprising one or more strains of the disclosure (eg, one or more agriculturally active Erwinia strains and/or one or more agriculturally active Pseudomonas strains).

Strains of the present disclosure can be incorporated into the inoculum composition in any suitable amount/concentration. The absolute amount/concentration sufficient to cause the desired effect is influenced by factors such as the type, size and volume of material to which the composition is applied, as well as storage conditions (e.g. temperature, relative humidity, duration). can be Those of ordinary skill in the art would understand how to select effective amounts/concentrations using routine dose-response studies.

In some embodiments, the inoculation compositions of the present disclosure contain about 1 x 10 ¹ to about 1 x 10 ¹⁵ colony forming units (cfu) per gram (g) and/or milliliter (mL) of the inoculation composition. A range of amounts of one or more strains of the disclosure. For example, about 1×10 ¹ , 1×10 ² , 1×10 ³ , 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ , 1 per gram and/or milliliter of inoculum composition. x10< ⁸ >, 1 x 10< ⁹ >, 1 x ¹⁰ <10>, 1 x 10< ¹¹ >, 1 x 10< ¹² >, or more cfu of pesticidal activity Erwinia (e.g. E. billigiae NRRL B-67766) and/or pesticidal Pseudomonas genera (eg, P. gesseardii NRRL B-67767 and/or P. libanesis NRRL B-67769). In some embodiments, the inoculum composition of the present disclosure contains at least about 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ , 1× per gram and/or milliliter of inoculum composition. 10 ⁸ , 1×10 ⁹ , 1×10 ¹⁰ , 1×10 ¹¹ , 1×10 ¹² cfu of pesticidal activity Erwinia (e.g. E. billigiae NRRL B-67766) and/or pesticidal Pseudomonas (e.g. P. gessardii) NRRL B-67767 and/or P. libanesis NRRL B-67769).

In some embodiments, strains of the present disclosure comprise about 0.1 to about 95% by weight of the inoculum composition. For example, the inoculum composition of the present disclosure has about 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5 , 3.75, 4, 4.25, 4.5, 4.75, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% by weight or more of pesticide activity Erwinia (e.g. E. billingae NRRL B-67766) and/or agrochemically active Pseudomonas (eg, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769). In some embodiments, agrochemically active Erwinia (e.g., E. billingae NRRL B-67766) and/or agrochemically active Pseudomonas (e.g., P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769) comprises from about 1 to about 25%, from about 5 to about 20%, from about 5 to 15%, from about 5 to about 10%, or from about 8 to about 12% by weight of the inoculum composition.

In some embodiments, an inoculum composition of the present disclosure is used to prevent and/or treat one or more pest infections/infestations when the inoculum composition is introduced into a plant growth medium (e.g., soil). It contains one or more strains of the present disclosure in an effective amount/concentration. For example, an inoculum composition of the present disclosure kills one or more pests (e.g., one or more fungal pests) and one or more pests (e.g., one or more E. billingiae in an amount/concentration effective to inhibit the growth of one or more pests (e.g., one or more fungal pests) and/or inhibit the reproduction/proliferation of one or more pests (e.g., one or more fungal pests). ) NRRL B-67766, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769.

In some embodiments, an inoculation composition of the present disclosure is effective to prevent and/or treat one or more pest infections/infestations when the inoculation composition is applied to a plant or plant part. An amount/concentration comprising one or more strains of the disclosure. For example, an inoculum composition of the present disclosure kills one or more pests (e.g., one or more fungal pests) when the composition is dressed on seeds or sprayed on plants. and in an amount/concentration effective to inhibit the growth of one or more pests (eg, one or more fungal pests) and/or inhibit the reproduction/reproduction of one or more pests, E. E. billingiae NRRL B-67766, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769.

In some embodiments, the inoculum composition of the present disclosure is in an amount/concentration effective to increase plant growth/yield when the inoculum composition is introduced into a plant growth medium (e.g., soil). , including one or more strains of the present disclosure. For example, an amount/concentration effective to enhance the growth and/or yield of plants (e.g., wheat) grown in fields treated with the composition compared to plants grown in untreated control fields. and may include E. billingiae NRRL B-67766, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769.

In some embodiments, an inoculum composition of the disclosure comprises one or more of the disclosure in an amount/concentration effective to enhance plant growth/yield when applied to a plant or plant part. Including stocks. For example, inoculum compositions of the present disclosure improve the growth and/or yield of plants (e.g., wheat) grown from seeds treated with the composition and/or plants (wheat) treated directly with the composition. E. billingiae NRRL B-67766, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769 in an amount/concentration effective to

In some embodiments, one or more cell-free filtrates of this disclosure comprise from about 0.1 to about 95% by weight of the composition. For example, the compositions of the present disclosure may contain about 0.1, 0.2, about 0.1, 0.2, acellular filtrate obtained from a culture comprising E. billingiae NRRL B-67766, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769. 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% by weight or more. In some embodiments, the cell-free filtrate of one or more pesticidally active Erwinia strains and/or one or more pesticidally active Pseudomonas strains comprises from about 1 to about 25% by weight of the composition, from about 5 to about 20 weight percent, about 5 to about 15 weight percent, about 5 to about 10 weight percent, or about 8 to about 12 weight percent.

In some embodiments, compositions of the present disclosure are effective in preventing and/or treating one or more pest infections/infestations when the compositions are introduced into a plant growth medium (e.g., soil). containing one or more cell-free filtrates in varying amounts/concentrations. For example, the compositions of the present disclosure kill one or more pests (e.g., one or more fungal pests) and one or more pests (e.g., one or more fungal pest) and/or in an amount/concentration effective to inhibit the growth/reproduction of one or more pests (e.g., one or more fungal pests). billingiae NRRL B-67766, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769.

In some embodiments, the compositions of the present disclosure are present in amounts/concentrations effective to prevent and/or treat one or more pest infections/infestations when the compositions are applied to a plant or plant part. , including one or more cell-free filtrates. For example, an inoculum composition of the present disclosure kills one or more pests (e.g., one or more fungal pests) when the composition is dressed on seeds or sprayed on plants, effective to control one or more pests (e.g. one or more fungal pests) and/or to control the reproduction/growth of one or more pests (e.g. one or more fungal pests) Cell-free filtrate of E. billingiae NRRL B-67766, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769 at the same amount/concentration can include

In some embodiments, the composition of the present disclosure comprises: Contains one or more cell extracts. For example, an inoculum composition of the present disclosure may improve the growth and/or yield of plants (e.g., wheat) grown in fields treated with the composition compared to, for example, plants grown in untreated control fields. E. billingiae NRRL B-67766, P. gessardii NRRL B-67767 and/or P. libanesis NRRL in amounts/concentrations effective to enhance dose It may contain a cell-free filtrate of B-67769.

In some embodiments, the compositions of the present disclosure contain one or more non-toxic ingredients in an amount/concentration effective to enhance plant growth/yield when the composition is applied to a plant or plant part. Contains cell filtrate. For example, the compositions of the present disclosure enhance the growth and/or yield of plants (e.g., wheat) grown from seeds treated with the composition and/or plants (e.g., wheat) treated directly with the composition. of E. billingiae NRRL B-67766, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769 in an amount/concentration effective to It may contain a cell-free filtrate.

In some embodiments, one or more cell extracts of this disclosure comprise about 0.1 to about 95% by weight of the composition. For example, the compositions of the present disclosure may be used in E. billingiae NRRL B-67766, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769 cultures. about 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75 , 4, 4.25, 4.5, 4.75, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 , 26, 27, 28, 29, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% by weight or more. In some embodiments, the cell extract of one or more pesticidally active Erwinia strains and/or one or more pesticidally active Pseudomonas strains comprises from about 1 to about 25% by weight of the composition, from about 5 to about 20 weight percent, about 5 to about 15 weight percent, about 5 to about 10 weight percent, or about 8 to about 12 weight percent.

In some embodiments, compositions of the present disclosure are effective to prevent and/or treat one or more pest infections/infestations when the compositions are introduced into a plant growth medium (e.g., soil). An amount/concentration comprising one or more cell extracts. For example, an inoculum composition of the present disclosure kills one or more pests (eg, one or more fungal pests) and kills one or more pests (eg, one or more fungal pests) when the composition is introduced into a field. E. villingii in an amount/concentration effective to inhibit the growth of one or more pests (e.g., one or more fungal pests) and/or inhibit the reproduction/proliferation of one or more pests (e.g., one or more fungal pests) (E. billingiae) NRRL B-67766, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769 cell extracts.

In some embodiments, the compositions of the present disclosure are present in amounts/concentrations effective to prevent and/or treat one or more pest infections/infestations when the compositions are applied to a plant or plant part. with one or more cell extracts. For example, the compositions of the present disclosure kill one or more pests (e.g., one or more fungal pests) when the compositions are dressed on seeds or sprayed on plants; Effective in inhibiting the growth of one or more pest species (e.g., one or more fungal pests) and/or inhibiting reproduction/reproduction of one or more pest species (e.g., one or more fungal pests) Cells obtained from E. billingiae NRRL B-67766, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769 at the same amount/concentration It may contain an extract.

In some embodiments, the compositions of the present disclosure contain one containing cell extracts of For example, the compositions of the present disclosure may reduce the growth and/or yield of plants (e.g., wheat) grown in fields treated with the compositions, e.g., when compared to plants grown in untreated control fields. E. billingiae NRRL B-67766, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B- in an amount/concentration effective to enhance Cell extracts obtained from 67769 may be included.

In some embodiments, the compositions of the present disclosure contain one or more cells in an effective amount/concentration to enhance plant growth/yield when the composition is applied to a plant or plant part. Contains extracts. For example, compositions of the present disclosure are effective in improving the growth/yield of plants (e.g., wheat) grown from seeds treated with the composition and/or plants (e.g., wheat) treated directly with the composition. cells derived from E. billingiae NRRL B-67766, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769 at the same amount/concentration It may contain an extract.

Compositions of the present disclosure can comprise any suitable carrier including, but not limited to, foliar-compatible carriers, seed-compatible carriers and soil-compatible carriers. The selection of a suitable carrier material will depend on the intended use and the microorganisms, cultures, cell-free filtrates and/or cell extracts present in the composition. In some embodiments, the carrier material is selected to provide the composition in liquid, gel, slurry, or solid form. In some embodiments, the carrier consists essentially of or will consist of one or more stabilizing compounds.

In some embodiments, compositions comprise one or more solid carriers. In some embodiments, the composition comprises one or more powders (eg wettable powders) and/or granules. Non-limiting examples of solid carriers include clays (eg, attapulgite clay, montmorillonite clay, etc.), peat-based powders and granules, freeze-dried powders, spray-dried powders, spray-freeze-dried powders and combinations thereof.

In some embodiments, the composition comprises one or more liquid and/or gel carriers.

According to some embodiments, the composition comprises one or more non-aqueous solvents. According to some embodiments, the composition comprises one or more aqueous solvents (eg, water). According to some embodiments, the aqueous solvent such as water is ethyl lactate, methyl soyate/ethyl lactate co-solvent blend (eg, STEPOSOL ^™ , Stepan), isopropanol, acetone, 1,2-propanediol. , n-alkylpyrrolidones (eg, AGSOLEX ^™ wetting agents; Ashland, Inc., Covington, Kentucky), petroleum-based oils (eg, AROMATIC ^™ and SOLVESSO ^™ fluids; ExxonMobil Chemical Company, Spring, TX), isoparaffinic hydrocarbons (e.g., ISOPAR ^™ Fluid; ExxonMobil Chemical Company, Spring, TX), cycloparaffinic hydrocarbons (e.g., NAPPAR ^™ 6; ExxonMobil Chemical Company, Spring, TX). ), mineral spirits (eg, VARSOL ^™ ; ExxonMobil Chemical Company, Spring, Tex.), and mineral oils (eg, paraffin oil). According to some embodiments, the composition comprises one or more inorganic solvents such as decane, dodecane, hexyl ether and nonane. According to some embodiments, the composition comprises one or more organic solvents such as acetone, dichloromethane, ethanol, hexane, methanol, propan-2-ol and trichlorethylene. Non-limiting examples of liquid/gel carriers include oils (e.g. mineral oil, olive oil, peanut oil, soybean oil, sunflower oil), polyethylene glycols (e.g. PEG 200, PEG 300, PEG 400, etc.), propylene glycol (e.g. PPG-9, PPG-10, PPG-17, PPG-20, PPG-26, etc.), ethoxylated alcohols (e.g., TOMADOL ^™ (Air Products and Chemicals, Inc. Allentown, PA), TERGITOL ^™ TERGITOL ^™ 15-S surfactants such as ® 15-S-9 (Dow Chemical Company, Midland, Mich.), isoparaffinic hydrocarbons (e.g., ISOPAR ^™ , ISOPAR ^™ L , ISOPAR ^™ M, ISOPAR ^™ V; ExxonMobil Chemical Company, Spring, TX), pentadecane, polysorbates (e.g., polysorbate 20, polysorbate 40, polysorbate 60, polysorbate 80, etc.), silicones (siloxanes, trisiloxanes etc.) and combinations thereof.In some embodiments, the carrier comprises, consists essentially of, or consists of dodecane.In some embodiments, the carrier comprises methyl soyate. In some embodiments, the carrier comprises, consists essentially of, or consists of one or more paraffin oils and/or waxes.

Further examples of carriers are found in BURGES, FORMULATION OF MICROBIAL BIOPESTICIDES: BENEFICAL MICROORGANISMS, NEMATODES and SEED TREATMENTS (Springer Science & Business Media) (2012); Inoue & Horikoshi, J. FERMENTATION BIOENG. 71(3): 194 (1991). can find.

The compositions of the present disclosure include, but are not limited to, maltodextrins, monosaccharides, disaccharides, oligosaccharides, sugar alcohols, humic acids, fulvic acids, malt extracts, peat extracts, betaine, proline, sarcosine, peptones, skimmed milk, Any suitable stabilizing compound may be included, including oxidation control ingredients, hygroscopic polymers and UV protectants.

In some embodiments, the composition contains one or more maltodextrins (e.g., about 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17 , one or more maltodextrins having a dextrose equivalent value (DEV) of 18, 19, 20, 21, 22, 23, 24 or 25). According to some embodiments, the composition comprises about 5 to about 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19 or 20, about 10 to about 11 , 12, 14, 15, 16, 17, 18, 19 or 20, or one or more maltodextrins having a DEV of from about 15 to about 16, 17, 18, 19 or 20. According to some embodiments, the composition comprises about 5 to about 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19 or 20, about 10 to about 11 , 12, 14, 15, 16, 17, 18, 19 or 20, or combinations of maltodextrins having a DEV of from about 15 to about 16, 17, 18, 19 or 20. Non-limiting examples of maltodextrins include MALTRIN® M040 (DEV=5; Molecular Weight=3600; Grain Processing Corporation, Muscatine, Iowa),
MALTRIN® M100 (DEV=10; MW=1800; Grain Processing Corporation, Muscatine, Iowa),
MALTRIN® M150 (DEV=15; MW=1200; Grain Processing Corporation, Muscatine, Iowa),
MALTRIN® M180 (DEV=18; MW=1050; Grain Processing Corporation, Muscatine, Iowa),
MALTRIN® M200 (DEV=20; MW=900; Grain Processing Corporation, Muscatine, Iowa),
MALTRIN® M250 (DEV=25; MW=720; Grain Processing Corporation, Muscatine, Iowa),
MALTRIN QD® M580 (DEV=16.5-19.9; Grain Processing Corporation, Muscatine, Iowa), MALTRIN QD® M585 (DEV=15.0-19.9; Grain Processing Corporation, Muscatine, Iowa),
GROBE® Plus 15DE (Ingredion Inc., Westchester, IL); and combinations thereof.

In some embodiments, the composition contains one or more monosaccharides (e.g., allose, altrose, arabinose, fructose, galactose, glucose, gulose, iodose, lyxose, mannose, ribose, talose, threose and/or xylose). )including. According to some embodiments the composition comprises glucose. According to some embodiments, the composition does not contain glucose.

In some embodiments, the composition comprises one or more disaccharides (e.g., cellobiose, chitobiose, gentiobiose, gentiobiulose, isomaltose, kojibiose, lactose, lactulose, laminaribiose, maltose (e.g., maltose- hydrate, anhydrous maltose), maltulose, mannobiose, melibiose, melibiulose, nigerose, palatinose, rutinose, rutinulose, sophorose, sucrose, trehalose, turanose and/or xylobiose). According to some embodiments, the composition comprises maltose. According to some embodiments, the composition is maltose-free. According to some embodiments, the composition comprises trehalose. According to some embodiments, the composition does not contain trehalose.

In some embodiments, the composition comprises one or more oligosaccharides (eg fructooligosaccharides, galactooligosaccharides, mannooligosaccharides and/or raffinose).

In some embodiments, the composition contains one or more sugar alcohols (e.g., arabitol, erythritol, fucitol, galactitol, glycerol, iditol, inositol, isomalt, lactitol, maltitol, maltotetritol, maltotritol). , mannitol, polyglycitol, ribitol, sorbitol, threitol, boremitol and/or xylitol).

In some embodiments, the composition comprises one or more humic acids (eg, one or more leonardite coal humic acids, lignite humic acids, peat mulch humic acids, and water-extracted humic acids). In some embodiments, the composition comprises ammonium humate, boron humate, potassium humate and/or sodium humate. In some embodiments, one or more of ammonium humate, boron humate, potassium humate and sodium humate are excluded from the composition. Non-limiting examples of humic acids that may be useful in embodiments of the present disclosure include MDL No. MFCD00147177 (CAS No. 1415-93-6), MDL No. MFCD00135560 (CAS No. 68131-04-4), MDL No. MFCS22495372 (CAS No. 68131-04-4). No. 68514-28-3), CAS No. 93924-35-7, and CAS No. 308067-45-0.

In some embodiments, the composition comprises one or more fulvic acids (eg, one or more leonardite coal fulvic acids, lignite fulvic acids, peat mulch fulvic acids and/or water-extracted fulvic acids). In some embodiments, the composition comprises ammonium fulvicate, boron fulvicate, potassium fulvicate and/or sodium fulvicate. In some embodiments, one or more of ammonium fulvicate, boron fulvicate, potassium fulvicate and sodium fulvicate are excluded from the compositions of the present disclosure. Non-limiting examples of fulvic acids that may be useful in embodiments of the present disclosure include MDL number MFCD09838488 (CAS number 479-66-3).

In some embodiments, the composition comprises one or more betaines (eg, trimethylglycine).
In some embodiments, the composition comprises one or more peptones (eg, bacterial peptones, meat peptones, milk peptones, plant peptones and yeast peptones).

In some embodiments, the composition includes one or more oxidation control ingredients (eg, one or more antioxidants and/or oxygen scavengers). According to some embodiments, the composition comprises one or more oxygen scavengers such as ascorbic acid, ascorbate, catechol and/or sodium bicarbonate. According to some embodiments, the composition contains one or more antioxidants such as ascorbic acid, ascorbyl palmitate, ascorbyl stearate, calcium ascorbate, carotenoids, lipoic acid, phenolic compounds (e.g. flavonoids, flavones , flavonols), potassium ascorbate, sodium ascorbate, thiols (eg, glutathione, lipoic acid, N-acetylcysteine), tocopherols, tocotrienols, ubiquinones and/or uric acid. Non-limiting examples of antioxidants include those that are soluble in cell membranes (eg, α-tocopherol (vitamin E), ascorbyl palmitate) and those that are soluble in water (eg, ascorbic acid and isomers , or sodium or potassium salts of ascorbic acid or isomers, glutathione, sodium or potassium salts of glutathione). In some embodiments, the use of membrane-soluble antioxidants requires the addition of one or more surfactants to properly disperse the antioxidants in the composition. According to some embodiments, the composition comprises ascorbic acid and/or glutathione.

In some embodiments, the composition comprises one or more hygroscopic polymers (e.g., hygroscopic agar, albumin, alginate, carrageenan, cellulose, gums (e.g., cellulose gum, guar gum, gum arabic, gum Sikunji, xanthan gum), methylcellulose, nylon, pectin, polyacrylic acid, polycaprolactone, polycarbonate, polyethylene glycol (PEG), polyethyleneimine (PEI), polylactide, polymethylacrylate (PMA), polyurethane, polyvinyl alcohol (PVA), polyvinylpyrrolidone ( PVP), propylene glycol, sodium carboxymethylcellulose and/or starch). Non-limiting examples of polymers include AGRIMER ^™ polymers (e.g., 30, AL-10 LC, AL-22, AT/ATF, VA 3E, VA 3I, VA 5E, VA 5I, VA 6, VA 6E, VA 7E, VA 7I, VEMA AN-216, VEMA AN-990, VEMA AN-1200, VEMA AN-1980, VEMA H- ^815MS ; Ashland Specialty Ingredients, Wilmington, Del.; Ingredients, Wilmington, DE); DISCO™ AG polymers (e.g., L-250, L-280, L-285, L-286, L-320, L-323, L-517, L-519, L -520, L800; Incotec Inc, Salinas, Calif.), KELZAN® Polymers (Bri-Chem Supply Ltd., Calgary, Alberta, Calif.), SEEDWORX ^™ Polymers (e.g., Bio 200; Aginnovation, LLC, Walnut Groove, Calif.), TICAXAN ^™ xanthan powders, such as PRE-HYDRATED® TICAXAN® Rapid-3 powders (TIC Gums, White Marsh, Md.), and combinations thereof. Additional examples of polymers can be found in Pouci et al., AM. J. AGRIC. BIOL. SCI. 3(1):299 (2008).

In certain embodiments, the inoculating composition of the present disclosure contains one or more UV protection agents [e.g., one or more aromatic amino acids (e.g., tryptophan, tyrosine), carotenoids, cinnamates, lignosulfonates (e.g., calcium lignosulfonate), , sodium lignosulfonate), melanin, mycosporin, polyphenols and/or salicylates). Non-limiting examples of UV protectants that may be useful in the compositions of the present disclosure include Borregarrd LignoTch™ lignosulfonates (e.g. Borresperse 3A, Borresperse CA, Borresperse NA, Marasperse AG, Norlig A, Norlig 11D, Ufoxane 3A, Ultrazine NA, Vanisperse CB; Borregaard Lignotech, Sarpsborg, Norway) and combinations thereof. Additional examples of UV protectants can be found in Burges, Formulation of Microbial Biopesticides: Beneficial Microorganisms, Nematodes and Seed Treatments (Springer Science & Business Media) (2012).

Additional examples of stabilizing compounds and combinations of carriers and stabilizing compounds can be found in International Patent Publication Nos. WO2017/044473, WO2017/044545, WO2017/116837, WO2017/116846. , WO2017/210163, WO2017/210166, WO2018/118740, WO2018/175681, WO2018/183491, WO2018/218008, WO2018/218016 and It can be found in the same pamphlet WO2018/218035. .

Compositions of the present disclosure include, but are not limited to, seaweed extracts (e.g., Ascophyllum nodosum (brown algae) extracts such as alginate, Ecklonia maxima (Ecklonia maxima) extract, etc.), myoinositol , glycine, and combinations thereof.

Compositions of the present disclosure may comprise any suitable biological extract including, but not limited to, bacterial extracts, fungal extracts and combinations thereof. Compositions of the present disclosure comprise one or more extracts from media containing one or more diazotrophs, phosphate-solubilizing microorganisms and/or biological pesticides (bio-pesticides). Some embodiments of the present disclosure include extracts from media containing one or more of the strains listed in Appendix A.

Compositions of the present disclosure include, but are not limited to, organic acids (such as acetic acid, citric acid, lactic acid, malic acid, taurine, etc.), macrominerals (such as phosphorus, calcium, magnesium, potassium, sodium, iron, etc.), trace minerals (such as boron, cobalt, chloride, chromium, copper, fluoride, iodine, manganese, molybdenum, selenium, zinc, etc.), vitamins (e.g., vitamin _A , vitamin _B complex (i.e., vitamin B1, vitamin B2, vitamin _B3 , vitamin _B5 , vitamin _B6 , vitamin B7, vitamin B8, vitamin _B9 , vitamin _B12 , choline), vitamin C _, vitamin D, vitamin E, vitamin K, carotenoids ₍ α-carotene, β- carotenes, cryptoxanthin, lutein, lycopene, zeaxanthin, etc.) and combinations thereof, hi certain embodiments, the inoculum composition of the present disclosure contains phosphorus, boron, chlorine, copper, , iron, manganese, molybdenum and/or zinc.

The inoculating composition of the present disclosure includes, but is not limited to, brevicomine, ceralure, codrelure, cuelure, dispalure, dominicalure, eugenol, frontalin ), gossyplure, grandlure, hexalure, ipsdienol, ipsenol, japonilure, latilure, lineatin, litlure, looplure, medlure megatomoic acid, methyl eugenol, moguchun, α-multistratin, muscalure, orfralure, oryctalure, ostramon, Any suitable pest attractant and/or feeding stimulant including rescalure, siglure, slucatol, trimedlure and/or trunc-call can include

Inoculum compositions of the present disclosure may comprise any suitable pesticide including, but not limited to, acaricides, fungicides, herbicides, insecticides, and nematicides.

Fungicides can be selected to provide effective control against a broad spectrum of plant pathogens (and fungi-like organisms), including but not limited to Ascomycetes, Basidiomycota Phylum Basidiomycetes, Phylum Chytridiomycetes, Phylum Deuteromycetes (syn. Fungi imperfecti), Peronosporomycetes (syn. Oomycetes), Necromycota (Plasmodiophoromycetes) and soil pathogens of the phylum Zygomycetes. According to some embodiments, the inoculum composition of the present disclosure contains Albugo (e.g., A. candida), Alternaria (e.g., A. alternata (A. alternata), Aspergillus (e.g. A. candidus, A. clavatus, A. flavus, A. fumigatus). fumigatus, A. parasiticus, A. restrictus, A. sojae, A. solani, Blumeria ) (e.g. B. graminis), Botrytis (e.g. B. cinerea; Botrytis), Cladosporum (e.g. C. Dosporioides (C. cladosporioides), Colletotrichum (e.g. C. acutatum, C. boninense, C. capsici, C. C. caudatum, C. coccodes, C. crassipes, C. dematium, C. destructivum, C. fragarie ( C. fragariae), C. gloeosporioides, C. graminicola, C. kehawee, C. lindemithianum, C. C. musae, C. orbiculare, C. spinaceae, C. sublineolum, C. trifolii, C. truncatum )), Fusarium Genus Fusarium (e.g. F. graminearum, F. moniliforme, F. oxysporum, F. roseum, F. tricinctum )), Helminthosporium, Magnaporthe (e.g., M. grisea; crabgrass blast), M. oryzae; lini) (e.g., M. lini), Mycoshaerella (e.g., M. graminicola), Nematospora, Penicillium (e.g., P. rugulosum, P. verrucosum), Phakopsora (e.g. P. pachyrhizi (soybean rust)), Phomopsis (root rot fungus) , Phytophtoria (e.g. P. infestans), Puccinia (e.g. P. graminis, P. striiformis) , P. tritici, P. triticina), Pucivinia (e.g. P. graministice), Pythium, Pythophthora ( Pytophthora), Rhizoctonia (e.g. R. solani), Scopulariopsis, Selerotinia, Thielaviopsis and/or Ustilago (e.g. A fungicide (or combination of fungicides) toxic to one or more strains of U. maydis )including. Additional examples of fungi targeted by inoculum compositions of the present disclosure can be found in Bradley, Managing Diseases, in Illinois Agronomy Handbook (2008).

The herbicide is effective against a broad spectrum of plants including, but not limited to, plants of the Asteraceae, Caryophyllaceae, Poaceae and Polygonaceae families. Can be selected to provide control. According to some embodiments, the inoculum composition of the present disclosure contains Echinochloa (e.g., E. brevipedicellata, E. callopus, E. cacoensis (E. chachoensis, E. colona, E. crus-galli, E. crus-pavonis, E. elliptica (E. elliptica), E. esculenta (barnyard millet), E. fumentacea (indica), E. glabrescens (barnyard barnyard), E. haploclada, E. E. helodes, E. holciformis, E. inundata, E. jaliscana, E. jubata, E. kimberleyensis kimberleynsis, E. lacunaria, E. macrandra, E. muricata, E. obtusiflora, E. oplismenoides ), E. orzyoides, E. paludigena, E. picta, E. pithopus, E. polustachya, E. E. praestans, E. pyramidalis, E. rotundiflora, E. stagnina, E. telmatophila, E. tourneliana turneriana, E. ugandensis, E. walteri], Fallopia [e.g. F. baldschuanica , F. japonica, F. sachalinensis], Stellaria (e.g. S. media) and/or Taraxacum ) [e.g., T. albidum, T. aphrogenes, T. brevicorniculatum, T. californicum, T. centraciatum (T. centrasiatum), T. ceratophorum, T. erythrospermum, T. farinosum, T. holmboei, T. josam (T. josum (kansai dandelion), T. kok-saghyz (rubber dandelion), T. laevigatum (red dandelion), T. officinale (officinale), T. platycarpum (T. platycarpum)] that is toxic to one or more strains of the herbicide (or combination of herbicides). Additional species of plants that can be targeted by the inoculum compositions of the present disclosure can be found in Hager, Weed Management, in Illinois Agronomy Handbook (2008) and Loux et al., Weed Control Guide for Ohio, Indiana and Illinois (2015). can be done.

Pesticides can include any suitable pesticide, including biological and chemical pesticides. Insecticides include, but are not limited to, Coleoptera, Dermaptera, Diptera, Hemiptera, Homoptera, Hymenoptera, Lepidoptera It can be selected to provide effective control over a broad spectrum of insects, including insects of the order Lepidoptera, Orthoptera and Thysanoptera. For example, the inoculum composition of the present disclosure may include Acrididae, Aleytodidae, Anobiidae, Anthomyiidae, Aphidae, Bostrichidae, Bean Weevil ( Bruchidae), Cecidomyiidae, Cerambycidae, Cercopidae, Chrysomelidae, Cicadelliae, Coccinellidae, Cryllotalpidae, Cucujidae , Curculionidae, Dermestidae, Elateridae, Gelechiidae, Lygaeidae, Meloidae, Membracidae, Miridae , Noctuidae, Pentatomidae, Pyralidae, Scarabaeidae, Silvanidae, Spinidae, Tenebrionidae and/or Thripidae
) insecticides that are toxic to insects. According to certain embodiments, the inoculum composition of the present disclosure contains Acalymma, Acanthaoscelides (e.g., A. obtectus), Anasa (e.g., , A. tristis), Anastrepha (e.g. A. ludens; Mexican fruit fly), Anoplophora (e.g. A. glabripennis; )), Anthonomus (e.g. A. eugenii), Acyrthosiphon (e.g. A. pisum), Bactrocera (e.g., B. dosalis; citrus fruit fly, Bemisia (e.g., B. argentifolii; silverleaf whitefly, B. tabaci; tobacco whitefly)), Brevicoryne (e.g. B. brassicae; Aphid radix), Bruchidius (e.g. B. atrolineatus), Bruchus (e.g. B. atrolineatus). Atmarius (B. atarius), B. dentipes (B. dentipes), B. lentis (B. lentis), B. pisorum (pea weevil) and/or B. rufipes), Carosoburucus Genus (Callosobruchus) [e.g., C. chinensis (bean weevil), C. maculatus (bean weevil), C. rhodesianus, C. sibinnotatus, C. theobromae], genus Caryedon (e.g., C. serratus), subfamily Chrysomelidae (Ca ssadinae), Ceratitis (e.g. C. capitata; medfly), Chrysomelinae, Circulifer (e.g. C. tenellus; sugar beet) , Criocerunae, Cryptocephalinae, Cryptolestes [e.g., C. ferrugineus; C. pusillis; C. pussilloides], Cylas (e.g. C. dormicarius), Delia (e.g. D. anticure (D. antiqua; onion fly), Diabrotica (corn rootworm), Diaphania (e.g. D. nitidalis), Diaphorina (e.g. D. citri (citrus psyllium), Donaciinae, Ephestia (e.g., E. cautella; E. elutella; Chamadara) E. keuhniella)], Epilachna (e.g. E. varivestris), Epiphyas (e.g. E. postivittana (E. postivittana), Eumolpinae, Galerucinae, Helicoverpa (e.g. H. zea) )), Heteroligus (e.g. H. meles), Lobesia (e.g. L. botrana )), Lamprosomatinae, Lasioderma (e.g. L. serricorne; tobacco beetle), Leptinotarsa (e.g. L. decemlineata; Colorado potato beetle) ), Leptoglossus, Liriomyza (e.g., L. trifolii), Manducca, Melittia (e.g., M. Cucurbitae (M. cucurbitae), Myzus (e.g. M. persicae; green peach aphid), Nezara (e.g. N. viridula; southern green stink bug) ), Orzaephilus (e.g. O. merator, O. surinamensis (Saw beetle)), Ostrinia (e.g. O. nubilalis; European corn borer), Phthorimaea (e.g. P. operculella), Pieris (e.g. P. rapae), Plodia ) (e.g. P. interpinctella), Plutella (e.g. P. xylostella), Popillia (e.g. P. japonica (P. japonica; beetle)), Prostephanus (e.g., P. truncates (P. truncates)), Psila (fly), Rhizopertha (e.g., R. dominica (R. dominica), Rhopalosiphum (e.g. R. maidis; Maize aphids), Sagrinae, Solenopsis (fire ants) (e.g., S. invicta (fire ants)), Spilopyrinae, Sitophilus [e.g., S. granaries, S. oryzae and/or S. zeamais], Sitotroga (e.g., S. cerealella) cerealella), Spodoptera (e.g. S. frugiperda), Stegobium (e.g. S. paniceum) , Synetinae, Tenebrio (e.g. T. malens and/or T. molitor), Thrips ) (e.g. T. tabaci; onion thrips), Trialeurodes (e.g. T. vaporariorum; whitefly), Tribolium [e.g. T. castaneum (T. castaneum and/or T. confsum, Trichoplusia (e.g. T. ni), Trogoderma (e.g. T. granarium) and Trogossitidae (e.g. T. mauritanicus). (or a combination of pesticides). Additional species of insects that can be targeted by the inoculum compositions of the present disclosure can be found in Capinera, Handbook of Vegetable Pests (2001) and Steffey & Gray, Managing Insect Pests, in Illinois Agronomy Handbook (2008).

Nematicides can be selected to provide effective control against a wide range of nematodes, including, but not limited to, plant-parasitic nematodes of the classes Chromadorea and Enoplea. . According to certain embodiments, the inoculation composition of the present disclosure contains Anguina, Aphelenchoides, Belonolaimus, Bursaphelenchus, Ditylenchus, Globodera Genus Globodera, Helicotylenchus, Heterodera, Hirschmanniella, Meloidogyne, Naccobus, Pratylenchus, Radopholus ), Rotylenshulus, Trichodorus, Tylenchulus and/or Xiphinema. agent combination). Additional examples of nematodes that can be targeted by the inoculum compositions of the present disclosure are found in CAPINERA, HANDBOOK OF VEGETABLE PESTS (2001) and Niblack, NEMATODES, ILLINOIS AGRONOMY HANDBOOK (2008).

In certain embodiments, an inoculum composition of the present disclosure comprises one or more chemical fungicides. Non-limiting examples of chemical fungicides include strobilurins such as azoxystrobin, cumethoxystrobin, spiderystrobin, dimoxystrobin, enesterobulin, fluoxastrobin, kresoxime-methyl, metminostrobin , oryzastrobin, picoxystrobin, pyraclostrobin, pyrametostrobin, pyraoxystrobin, pyribencarb, trifloxystrobin, 2-[2-(2,5-dimethylphenoxymethyl)phenyl]-3-methoxy -acrylic acid methyl ester and 2-(2-(3-(2,6-dichlorophenyl)-1-methyl-allylideneaminooxymethyl)phenyl)-2-methoxyimino-N-methyl-acetamide; carboxamides, such as , carboxanilides (e.g. benalaxyl, benalaxyl-M, benodanil, bixafene, boscalid, carboxin, fenfram, fenhexamide, flutolanil, fluxapyroxad, furametpyr, isopyrazam, isotianil, chiralaxyl, mepronyl, metalaxyl, metalaxyl-M ( mefenoxam), oflace, oxadixyl, oxycarboxin, penflufen, penthiopyrad, sedaxane, tecloftalam, thifluzamide, thiazinyl, 2-amino-4-methyl-thiazole-5-carboxanilide, N-(4'-trifluoromethylthiobiphenyl- 2-yl)-3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, N-(2-(1,3,3-trimethylbutyl)phenyl)-1,3-dimethyl-5-fluoro- 1H-pyrazole-4-carboxamide), carboxylic acid morpholides (e.g. dimethomorph, flumorph, pyrimorph), benzoic acid amides (e.g. flumetover, fluopicolide, fluopyram, zoxamide), carpropamide, dicyclomet, mandipropamide, oxytetracycline, silthiopham and N-(6 -methoxypyridin-3-yl)cyclopropanecarboxylic acid amide; azoles such as triazoles (eg azaconazole, bitertanol, bromconazole, cyproconazole, difenoconazole, diniconazole, diniconazole-M, epoxiconazole, fenbuconazole, fluquinconazole, flusilazole, flutriafol, hexaconazole, imibenconazole, ipconazole le, metconazole, microbutanil, oxypoconazole, paclobutrazole, penconazole, propiconazole, prothioconazole, simeconazole, tebuconazole, tetraconazole, triadimefon, triadimenol, triticonazole, uniconazole) and imidazoles ( Cyazofamid, Imazalil, Pefurazoate, Prochloraz, Triflumizole); heterocyclic compounds such as pyridines (eg Fluazinam, Pyrifenox (see D1b), 3-[5-(4-chlorophenyl)-2,3-dimethyl) isoxazolidin-3-yl]pyridine, 3-[5-(4-methylphenyl)-2,3-dimethylisoxazolidin-3-yl]pyridine), pyrimidines (e.g. bupirimate, cyprodinil, diflumethrim, fenarimol, ferimzone, mepanipyrim, nitrapyrine, nuarimol, pyrimethanil), piperazines (e.g. trifoline), pyrroles (e.g. fenpicronil, fludioxonil), morpholines (e.g. aldimorph, dodemorph, dodemorph acetate, fenpropimorph, tridemorph), piperidines (e.g. fenpro pidgin); dicarboximides (e.g. fluoroimide, iprodione, procymidone, vinclozoline), non-aromatic 5-membered heterocyclic compounds (e.g. famoxadone, phenamidone, flutianil, octhilinone, probenazole, 5-amino-2-isopropyl-3-oxo -4-orthotolyl-2,3-dihydro-pyrazole-1-carbothioic acid S-allyl ester), acibenzolar-S-methyl, amethoctraazine, amisulbrom, anilazine, blasticidin-S, captafol, captan, quinomethionate, dazomet, debacarb , diclomedine, difenzocort, difenzocort-methylsulfate, phenoxanyl, folpet, oxolinic acid, piperalin, proquinazide, pyroquilone, quinoxyphene, triazoxide, tricyclazole, 2-butoxy-6-iodo-3-propylchromen-4-one, 5-chloro -1-(4,6-dimethoxypyrimidin-2-yl)-2-methyl-1H-benzimidazole, and 5-chloro-7-(4-methylpiperidin-1-yl)-6-(2,4, 6-trifluorophenyl)-[1,2,4]triazolo-[1,5-a]pyrimidine; benzimida Soles, such as carbendazim; and other actives, such as guanidines (eg, guanidine, dodine, dodine free base, guazatine, guazathine acetate, iminoctazine), iminoctazine triacetate and iminoctazine-tris (albesylate); antibiotics (e.g. kasugamycin, kasugamycin hydrochloride hydrate, streptomycin, polyoxins and validamycin A), nitrophenyl derivatives (e.g. binapacryl, dichlorane, dinobutone, dinocap, nitrotal-isopropyl, technazen); organometallic compounds (e.g. fenthine salts, e.g. fenthin acetate, fenthin chloride, fenthin hydroxide); sulfur-containing heterocyclic compounds (e.g. dithianone, isoprothiolane); organophosphorus compounds (e.g. edifenphos, fosetyl, fosetyl-aluminium, iprobenfos, phosphoric acid and its salts, pyrazophos , tolclofos-methyl); organochlorine compounds (e.g. chlorothalonil, diclofluanid, dichlorophene, fursulfamide, hexachlorobenzene, pensikuurone, pentachlorophenol and its salts, phthalide, quintozene, thiophanate-methyl, thiophanate, tolylfluani N-(4-chloro-2-nitrophenyl)-N-ethyl-4-methyl-benzenesulfonamide) and inorganic active substances (eg Bordeaux mixture, copper acetate, copper hydroxide, copper oxychloride, basic copper sulfate, sulfur) as well as combinations thereof.

In some embodiments, the compositions of the present disclosure contain acibenzolar-S-methyl, azoxystrobin, benalaxil, bixafene, boscalid, carbendazim, cyproconazole, dimethomorph, epoxiconazole, fludioxonil, fluopyram, fluoxafen. strobin, flutianil, flutolanil, fluxapyroxad, fosetyl-Al, ipconazole, isopyrazam, cresoxime-methyl, mefenoxam, metalaxyl, metconazole, microbutanil, oryzastrobin, penflufen, penthiopyrad, picoxystrobin, propiconazole, proti Including oconazole, pyraclostrobin, sedaxane, silthiofam, tebuconazole, thiabendazole, thifluzamide, thiophanate, tolclofos-methyl, trifloxystrobin and triticonazole. In some embodiments, the compositions of the present disclosure contain azoxystrobin, pyraclostrobin, fluoxastrobin, trifloxystrobin, ipconazole, prothioconazole, sedaxane, fludioxonil, metalaxyl, mefenoxam, thiabendazole, flu Including xapyroxad and/or fluopyram. In some embodiments, the compositions of the present disclosure contain one or more aromatic hydrocarbons, benzimidazoles, benzthiadiazoles, carboxamides, carboxylic acid amides, morpholines, phenylamides, phosphonates, quinone external inhibitors (e.g., strobilurine). , thiazolidines, thiophanates, thiophenecarboxamides and/or triazoles.

In some embodiments, compositions of the present disclosure comprise one or more chemical herbicides. Non-limiting examples of chemical herbicides include 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), ametrine, amicarbazone, aminocyclo pyrachlor, acetochlor, acifluorfen, alachlor, atrazine, azafenidine, bentazone, benzofenap, bifenox, bromacil, bromoxynil, butachlor, butaphenacyl, butroxidim, carfentrazone-ethyl, chlorimuron, chlorotoluron, clethodim, clodinafop, clomazone, Cyanadine, cycloxydim, cyhalofop, desmedifam, desmethrin, dicamba, diclofop, diflufenican, dimeflon, diuron, dithiopyr, ethofumesate, fenoxaprop, fluazifop, fluazifop-P, flufenacet, fluometuron, flufenpyr-ethyl, flumicrolac-pentyl, flumioxazin , fluoroglycofen, fluthiacet-methyl, fomesafe, fomesafen, foramsulfuron, glyphosate, glufosinate, haloxyfop, hexazinone, imazamox, imazaquin, imazethapyr, indadifam, iodosulfuron, ioxynil, isoproturon, isoxaflutole, lactofen, linuron , mecoprop, mecoprop-P, mesosulfuron, mesotrione, metamitron, metazochlor, methibenzuron, metolachlor (and S-metolachlor), metoxuron, metribuzine, monolinuron, oxadiargyl, oxadiazone, oxadiclomefone, oxyfluorfen, fenmedifam, pretilachlor , profoxydim, promethone, prometry, propacrol, propanil, propaquizafop, propisochlor, pyraflufen-ethyl, pyrazone, pyrazolinate, pyrazoxifene, pyridate, quizalofop, quizalofop-P (e.g., quizalofop-ethyl, quizalofop- P-ethyl, clodinafop-propargyl, cyhalofop-butyl, diclofop-methyl, fenoxaprop-p-ethyl, fluazifop-p-butyl, haloxyfop-methyl, haloxyfop-R-methyl), saflufenacyl, sethoxydim, siduron, simazine, simetrine , sulcotrione, sulfentrazone, Tebuti uron, tembotrione, tepraloxydim, terbacil, terbumetone, terbuthylazine, saxtomin (eg, saxtomin described in US Pat. No. 7,989,393), thenylchlor, thiencarbazone-methyl, tralkoxydim, triclopyr, trietadine, topramezone, salts and esters thereof racemic mixtures thereof and racemic resolved isomers thereof and combinations thereof. In some embodiments, the compositions of the present disclosure contain acetochlor, clethodim, dicamba, flumioxazin, fomesafen, glyphosate, glufosinate, dicamba, mesotrione, quizalofop, saflufenacil, sulcotrione, S-3100 and/or 2,4- including D. In some embodiments, the compositions of the present disclosure contain glyphosate, glufosinate, dicamba, 2,4-D, acetochlor, metolachlor, pyroxasulfone, flumioxazin, fomesafen, lactofen, metribuzine, mesotrione, and/or ethyl 2 -((3-(2-chloro-4-fluoro-5-(3-methyl-2,6-dioxo-4-(trifluoromethyl)-2,3-dihydropyrimidin-1(6H)-yl)phenoxy ) pyridin-2-yl)oxy) acetate. In certain embodiments, the inoculum composition of the present disclosure contains one or more of an acetyl CoA carboxylase (Accase) inhibitor, an acetolactate synthase (ALS) inhibitor, an acetohydroxy acid synthase (AHAS) inhibitor, a photosystem II inhibitor. agents, photosystem I inhibitors, protoporphyrinogen oxidase (PPO or Protox) inhibitors, carotenoid biosynthesis inhibitors, enolpyruvylshikimate 3-phosphate (EPSP) synthase inhibitors, glutamine synthetase inhibitors, dihydropteroate synthetase inhibitors, mitotic inhibitors, 4-hydroxyphenylpyruvate dioxygenase (4-HPPD) inhibitors, synthetic auxins, auxin herbicide salts, auxin transport inhibitors, nucleic acid inhibitors and/or their It includes one or more salts, esters, racemic mixtures and/or racemic resolution isomers thereof.

In some embodiments, compositions of the present disclosure comprise one or more chemical pesticides and/or nematicides. Non-limiting examples of chemical insecticides and nematicides include abamectin, acrinathrin, aldicarb, aldoxycarb, alpha-cypermethrin, beta-cyfluthrin, bifenthrin, cyhalothrin, cypermethrin, deltamethrin, esfenvalerate, etho Fenprox, Fenpropathrin, Fenvalerate, Flucythrinate, Fosthiazate, λ-Cyhalothrin, γ-Cyhalothrin, Permethrin, τ-Fluvalinate, Transfluthrin, ζ-Cypermethrin, Cyfluthrin, Bifenthrin, Tefluthrin, Eflucilanato, Fubufen prox, pyrethrin, resmethrin, imidacloprid, acetamiprid, thiamethoxam, nitenpyram, thiacloprid, dinotefuran, clothianidin, chlorfluazuron, diflubenzuron, lufenuron, teflubenzuron, triflumuron, novaluron, flufenoxuron, hexaflumuron, bistrifluron, noviflumuron, buprofezin , cyromazine, methoxyfenozide, tebufenozide, halofenozide, chromafenozide, endosulfan, fipronil, ethiprole, pyrafluprole, piriprole, flubendiamide, chlorantraniliprole, cyazypyr, emamectin, emamectin benzoate, abamectin, ivermectin, milbemectin, Lepimectin, tebufenpyrad, fenpyroximate, pyridaben, fenazaquin, pyrimidifen, tolfenpyrad, dicofol, cyenopyrafen, cyflumetofen, acequinocyl, fluacrypyrim, bifenazate, diafenthiuron, etoxazole, clofentedine, spinosad, triaracene, tetradifone, propargite, hexythiazox, bromopropylate , quinomethionate, amitraz, pyrifluquinazone, pymetrozine, flonicamid, pyriproxyfen, diphenolane, chlorfenapyr, metaflumizone, indoxacarb, chlorpyrifos, spirodiclofen, spiromesifen, spirotetramat, pyridalyl, spinktoram, acephate, triazophos, profenophos, Oxamyl, spinetoram, fenamiphos, fenamipcrotiaphos, 4-{[(6-chloropyrid-3-yl)methyl](2,2-difluoroethyl)amino}furan -2(5H)-one, 3,5-disubstituted-1,2,4-oxadiazole compounds, 3-phenyl-5-(thien-2-yl)-1,2,4-oxadiazole, Cadusafos, carbaryl, carbofuran, ethprophos, thiodicarb, aldicarb, aldoxycarb, methamidophos, methiocarb, sulxaflor, methamidophos, cyantraniliprole and thioxazaphene and combinations thereof. In certain embodiments, the inoculating composition of the present disclosure contains abamectin, aldicarb, aldoxycarb, bifenthrin, carbofuran, chlorantraniliprole, clothianidin, cyfluthrin, cyhalothrin, cypermethrin, cyantraniliprole, deltamethrin, dinotefuran, emamectin , ethiprole, fenamiphos, fipronil, flubendiamide, fosthiazate, imidacloprid, ivermectin, lambda-cyhalothrin, milbemectin, nitenpyram, oxamyl, permethrin, spinetoram, spinosad, spirodiclofen, spirotetramat, tefluthrin, thiacloprid, thiamethoxam and/or thiodicarb include. In some embodiments, the compositions of the present disclosure contain one or more carbamates, diamides, macrocyclic lactones, neonicotinoids, organophosphates, phenylpyrazoles, pyrethrins, spinosyns, synthetic pyrethroids, tetronic acid and/or tetramic acid. including. In some embodiments, the compositions of the present disclosure comprise an insecticide selected from the group consisting of clothianidin, thiamethoxam, imidacloprid, cyantraniliprole, chlorantraniliprole, fluopyram and thioxazaphene.

In certain embodiments, compositions of the present disclosure comprise one or more biopesticides (eg, one or more biofungicides, bioinsecticides and/or bionematicides). Examples of microbial strains exhibiting biopesticide activity are listed in Appendix A, along with strains exhibiting nitrogen-fixing activity, phosphate-solubilizing activity, and the like. Additional examples of pesticides are in Bradley, Managing Diseases, in Illinois Agronomy Handbook (2008); Hager, Weed Management, in Illinois Agronomy Handbook (2008); Loux et al., Weed Control Guide for Ohio, Indiana and Illinois (2015); , Nematodes, in Illinois Agronomy Handbook (2008); and Steffey & Gray, Managing Insect Pests, in Illinois Agronomy Handbook (2008).

The compositions of the present disclosure contain lipo-chitooligosaccharides (LCO), chitin oligomers, chitosan oligomers, chitin compounds, flavonoids, non-flavonoid nod gene inducers, jasmonic acid or derivatives thereof, linoleic acid or derivatives thereof, linolenic acid or Any suitable plant signal molecule may be included, including derivatives and karrikin.

Compositions of the present disclosure may comprise any suitable LCO. LCO, sometimes called the symbiotic nodulation (Nod) signal or Nod factor, is a β-l,4-linked-N-acetyl-D-glucosamine (“GlcNAc ”) consists of an oligosaccharide backbone of residues. LCOs differ in the number of GlcNAc residues in the backbone, the length and degree of saturation of the fatty acyl chains and the substitution of reducing and non-reducing sugar residues. See, e.g., Denarie et al., Ann. Rev. Biochem. 65:503 (1996); Hamel et al., Planta 232:787 (2010); Prome et al., Pure & Appl. Chem. 70(1):55 (1998). sea bream.

In some embodiments, the inoculum composition of the present disclosure comprises one or more LCOs represented by Formula I:

wherein G is a hexosamine which can be substituted, for example, by an acetyl group on nitrogen, by a sulfate group on oxygen, by an acetyl group and/or by an ether group; R ₁ , R ₂ , R ₃ , R ₅ , R ₆ and R ₇ may be the same or different, and may be H, CH ₃ CO--, C _x H _y CO--, where x is an integer from 0 to 17 and y is from 1 to 35 ), or any other acyl group, such as carbamoyl; R ₄ represents a saturated aliphatic chain or a mono-, di- or trisubstituted aliphatic chain containing at least 12 carbon atoms represents a chain; and n is an integer from 1-4.

In some embodiments, the inoculum composition of the present disclosure comprises one or more LCOs represented by Formula II:

wherein R represents H or CH ₃ CO-- and n equals 2 or 3. See, for example, US Pat. No. 5,549,718. _{A number} of _LCOs from soybean rhizobia Bradyrhizobium japonicum have been described, _e.g. contains BjNod-V (A _C , C _16:0 ) ("V" indicates the presence of five N-acetylglucosamines, "Ac" indicates the presence of acetylation; the number following "C" is , indicates the number of carbons in the fatty acid side chain, and the number following ":" indicates the number of double bonds). See, for example, US Pat. Nos. 5,175,149 and 5,321,011. Additional LCOs obtained from bacterial strains include NodRM, NodRM-1, NodRM-3. When acetylated (R=CH ₃ CO—) they become AcNodRM-1 and AcNodRM-3, respectively (US Pat. No. 5,545,718).

In some embodiments, an inoculum composition of the present disclosure comprises one or more LCOs represented by Formula III:

wherein n = 1 or 2; _R1 represents C16, C16:0, C16:1, C16:2, C18:0, C18:1Δ9Z or C18:1Δ11Z; and _R2 is hydrogen or SO Represents _3H .

In some embodiments, the inoculum composition of the present disclosure comprises one or more LCOs represented by Formula IV:

where R ₁ is C14:0, 3-OH-C14:0, iso-C15:0, C16:0, 3-OH-C16:0, iso-C15:0, C16:1, C16:2, C16 :3, iso-C17:0, iso-C17:1, C18:0, 3-OH-C18:0, C18:0/3-OH, C18:1, OH-C18:1, C18:2, C18:3 , C18:4, C19:1 carbamoyl, C20:0, C20:1, 3-OH-C20:1, C20:1/3-OH, C20:2, C20:3, C22:1 and C18-26 ( ω-1)-OH (according to D'Haeze et al., Glycobiology 12:79R-105R (2002), this includes C18, C20, C22, C24 and C26 hydroxylated species, C16:1Δ9, C16:2 (Δ2 ,9) and C16: ₃ (Δ2,4,9) species); _R2 represents hydrogen or methyl; R3 represents hydrogen, acetyl or carbamoyl; _R4 represents hydrogen, acetyl or carbamoyl R5 represents hydrogen, acetyl or carbamoyl; R6 represents hydrogen, arabinosyl, fucosyl, acetyl, SO3H _, sulfate ester, ₃ -OS-2-O- _MeFuc , 2-O-MeFuc and 4-O _-AcFuc ; _R7 represents hydrogen, _mannosyl or glycerol; R8 represents hydrogen, methyl or _-CH2OH ; R9 represents hydrogen, arabinosyl or fucosyl _; R10 represents hydrogen, acetyl or fucosyl and n represents 0, 1, 2 or. A native LCO encompassed by this structure is described in D'Haeze et al., supra.

Additional examples of LCOs that may be useful in the compositions and methods of the present disclosure are provided below as Structures V-XXXIII.

LCO can be obtained from any suitable source. In some embodiments, LCOs of the present disclosure are obtained (ie, isolated and/or purified) from bacterial strains. For example, in some embodiments, the compositions of the present disclosure contain Azorhizobium, Bradyrhizobium (e.g., B. japonicum), Mesorhizobium, Rhizobium (e.g., R. leguminosarum), or Sinorhizobium (e.g., R. leguminosarum). , S. 1 meliloti). In some embodiments, the LCO is obtained (ie, isolated and/or purified) from mycorrhizal fungi. For example, in some embodiments, compositions of the present disclosure comprise one or more LCOs obtained from strains of Glomerocycota (eg, Glomus intraaradicus). See, for example, WO 2010/049751, where LCOs are referred to as "Myc factors". In some embodiments, the LCO is synthetic. For example, in some embodiments, compositions of the present disclosure comprise one or more of the synthetic LCOs described in WO 2005/063784, WO 2007/117500 and/or WO 2008/071674. In some embodiments, the synthetic LCO comprises one or more modifications or substitutions, such as those described in Spain, CRIT. REV. PLANT SCI. 54:257 (2000) and d'Haeze, supra; Precursors for the production of LCOs, such as chitin oligomers, which are themselves useful as plant signaling molecules, can be synthesized by genetically engineered organisms. See, eg, Samain et al., Carbohydrate Res. 302:35 (1997); Cottaz et al., Meth. Eng. 7(4):311 (2005); and Samain et al., J. Biotechnol. 72:33 (1999). .

The compositions and methods of the present disclosure may include analogues, derivatives, hydrates, isotopes, salts and/or solvates of LCO. Thus, in some embodiments, the compositions of the present disclosure are represented by one or more of Formulas I-IV and/or Structures V-XXXIII 1, 2, 3, 4, 5, 6, 7 , 8, 9, 10 or more LCOs, and/or 1, 2, 3, 4, 5, 6, 7 represented by one or more of Formulas I-IV and/or Structures V-XXXIII. , 8, 9, 10 or more LCO analogues, derivatives, hydrates, isomers, salts and/or solvates.

LCO (and its derivatives) are available in forms of varying degrees of purity and can be used alone or in the form of cultures of LCO-producing bacteria or fungi. In some embodiments, the LCO contained in the compositions of the present disclosure is at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5% or more pure.

Compositions of the present disclosure may comprise any suitable chitin and/or chitosan oligomers. 12(6):79R (2002); Demont-Caulet et al., Plant Physiol. 120(1):83 (1999); Hanel et al., Planta 232:787 (2010); Muller et al. , Plant Physiol. 124:733 (2000); Robina et al., Tetrahedron 58:521-530 (2002); Rouge et al., Docking of Chitin Oligomers and Nod Factors on Lectin Domains of the LysM-RLK Receptors in the Medicago-Rhizobium Symbiosis, in The Molecular Immunology of Complex Carbohydrates-3 (Springer Science, 2011); Van der Holst et al., Curr. Opin. Struc. Biol. 11:608 (2001); Wan et al., Plant Cell 21:1053 (2009); See patent application PCT/FI00/00803 (2000).

In some embodiments, the inoculum composition of the present disclosure comprises one or more chitin oligosaccharides represented by Formula XXXIV:

wherein R ₁ represents hydrogen or methyl; R ₂ represents hydrogen or methyl; R ₃ represents hydrogen, acetyl or _carbamoyl ; R ₄ represents hydrogen, acetyl or carbamoyl; or carbamoyl; R6 represents hydrogen, arabinosyl, fucosyl, acetyl, sulfate, 3-OS-2-O-MeFuc, 2-O-MeFuc and 4-O- _AcFuc ; _R7 represents hydrogen, mannosyl or R8 represents hydrogen, methyl or _-CH2OH ; R9 represents hydrogen, _arabinisyl or fucosyl _; R10 represents hydrogen, acetyl or fucosyl; and n is ₀ , 1, 2 or 3 represents

In some embodiments, the inoculum composition of the present disclosure comprises one or more chitin oligosaccharides represented by Formula XXXV:

wherein n=1 or ₂ ; _R1 represents hydrogen or methyl; and _R2 represents hydrogen or SO3H.

Further examples of oligosaccharides (and derivatives thereof) that may be useful in the compositions and methods of this disclosure are provided below as structures XXXVI-LXXXIII:

In some embodiments, the inoculum composition of this disclosure comprises one or more of the oligosaccharides shown above as structures XXXVI-LXXXIII in deacetylated form (e.g., one or more acetyl groups is removed and optionally substituted with a hydrogen or methyl group).

Chitin oligosaccharides and chitosan oligosaccharides can be obtained from any suitable source. Chitin oligosaccharides and chitosan oligosaccharides can be harvested from chitin/chitosan (eg, Aam et al., Mar. Drugs 8:1482 (2010); D'Haeze et al., Glycobiol. 12(6):79R (2002) Demont-Caulet et al., Plant Physiol. 120(1):83 (1999); Hanel et al., Planta 232:787 (2010); Limpanavech et al., Scientia Horticulturae 116:65 (2008); Lodhi et al., BioMed Res. Intl. Vol. 2014 Art. 654913 (March 2014); Mourya et al., Polymer Sci. 53(7):583 (2011); Muller et al., Plant Physiol.124:733 (2000); Robina et al., Tetrahedron 58:521 (2002) Rouge et al., "The Molecular Immunology of Complex Carbohydrates", in Advances in Experimental Medicine and Biology (Springer Science, 2011); Van der Holst et al., Curr. Opin. Struc. Biol. 11:608 (2001); Wan et al. See Plant Cell 21:1053 (2009); Xia et al., Food Hydrocolloids 25:170 (2011); PCT/F100/00803 (2000)). They can be produced synthetically (eg, Cottaz et al., Meth. Eng. 7(4):311 (2005); Samain et al., Carbohydrate Res. 302:35 (1997); Samain et al., J. Biotechnol. 72:33 (1999)). In some embodiments, they are derived from naturally occurring LCOs. For example, in some embodiments, the compositions of the present disclosure include Azorhizobium, Bradyrhizobium (e.g., B. japonicum), Mesorhizobium, Rhizobium (e.g., R. leguminosarum), or obtained from LCO obtained (i.e. isolated and/or purified) from the genus Sinorhizobium (e.g. S. meliloti), or Glomeromycota (e.g. Glomus intraradicus) fungi or chitin/chitosan. In some embodiments, the compositions of the present disclosure contain Azorhizobium, Bradyrhizobium (e.g., B. japonicum), Mesorhizobium, Rhizobium ( obtained from strains of the genus Sinorhizobium (e.g. S. meliloti), or Glomeromycota (e.g. Glomus intraradicus). ie, isolated and/or purified) comprises one or more chitin oligosaccharides and/or chitosan oligosaccharides. In some embodiments, the chitin oligosaccharides and/or chitosan oligosaccharides are of Formulas I-IV and/or Structures V-XXXIII, except that the pendant fatty acid is replaced by a hydrogen or methyl group. It may comprise one or more chitin oligosaccharides represented by one or more.

The compositions of the present disclosure may comprise analogs, derivatives, hydrates, isomers, salts and/or solvates of chitin oligosaccharides and/or chitosan oligosaccharides. Thus, in some embodiments, the compositions of the present disclosure are represented by one or more of Formulas XXXIV-XXXV and/or one or more of Structures XXXVI-LXXXIII 1, 2, 3, 4 , 5, 6, 7, 8, 9, 10 or more chitin oligosaccharides, and/or 1, 2, 3 represented by one or more of formulas XXXIV-XXXV and/or structures XXXVI-LXXXIII. , 4, 5, 6, 7, 8, 9, 10 or more chitin oligosaccharide analogs, derivatives, hydrates, isomers, salts and/or solvates. be.

Chitin oligosaccharides and chitosan oligosaccharides (and their analogs, derivatives, hydrates, isomers, salts and/or hydrates) are available in various purity forms and can be used alone or for CO production. They are available in the form of bacterial or fungal cultures. In some embodiments, the chitin oligosaccharides and/or chitosan oligosaccharides contained in the compositions of the present disclosure are at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85% %, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5% or more pure.

The compositions of the present disclosure include, but are not limited to, chitin IUPAC: N-[5-[[3-acetylamino-4,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]methoxymethyl-2-[ [5-acetylamino-4,6-dihydroxy-2-(hydroxymethyl)oxan-3-yl]methoxymethyl]-4-hydroxy-6-(hydroxymethyl)oxan-3-yl]ethanamide, chitosan IUPAC: 5 -amino-6-[5-amino-6-[5-amino-4,6-dihydroxy-2(hydroxymethyl)oxan-3-yl]oxy-4-hydroxy-2-(hydroxymethyl)oxane-3- Any suitable chitinous compound can be included, including yl]oxy-2(hydroxymethyl)oxane-3,4-diol, and isomers, salts and solvates thereof.

Chitin and chitosan are major components of fungal cell walls and insect and crustacean exoskeletons, and they are composed of GlcNAc residues.

Chitin and chitosan are commercially available or can be prepared from insect, crustacean exoskeletons or fungal cell walls. Methods for preparing chitin and chitosan are well known in the art. See, for example, U.S. Pat. Nos. 4,536,207 (preparation from crustacean shells) and 5,965,545 (preparation from crab shells and hydrolysis of commercially available chitosan); Pochanavanich et al., Lett. Appl. Microbiol. 35:17 (2002) (preparation from fungal cell walls).

Deacetylated chitin and chitosan have deacetylation ranging from less than 35% to more than 90% and cover a broad spectrum of molecular weights, such as low molecular weight chitosan oligomers of less than 15 kD and chitin oligomers of 0.5-2.0 kD. "utility grade" chitosans with a molecular weight of about 15 kD; and high molecular weight chitosans up to 70 kD. Chitin and chitosan compositions formulated for seed treatment are commercially available. Commercial products include, for example, ELEXA® (Plant Defense Boosters, Inc.) and BEYOND™ (Agrihouse, Inc.).

The compositions of the present disclosure may be any suitable flavonoids including, but not limited to, anthocyanidins, anthoxanthins, chalcone, coumarins, flavanones, flavanonols, flavans and isoflavonoids, and analogs, derivatives, hydrates thereof. , isomers, polymers, salts and solvates.

Flavonoids are phenolic compounds having the general structure of two aromatic rings linked by a three carbon bridge. Classes of flavonoids include those known in the art. See, eg, Jain et al., J. Plant Biochem. & Biotechnol. 11:1 (2002); Shaw et al., Environ. Microbiol. 11:1867 (2006). Flavonoid compounds are commercially available from Novozyme BioAg, Saskatoon, Canada; Natland International, Research Triangle Park, NC, USA; MP Biomedicals, Irvine, CA; LC Laboratories, Woburn, MA. Flavonoid compounds can be isolated from plants or seeds, for example, as described in US Pat. Nos. 5,702,752; 5,990,291; and 6,146,668. . Flavonoid compounds can also be produced by genetically engineered organisms such as yeast, as described by Ralston et al., Plant Physiol 137:1375 (2005).

In some embodiments, compositions of the present disclosure comprise one or more anthocyanidins. According to some embodiments, the composition comprises cyanidin, delphinidin, malvidin, pelargonidin, peonidin and/or petunidin.

In some embodiments, compositions of the present disclosure comprise one or more anthoxanthins. According to some embodiments, the composition comprises one or more flavones (e.g., apigenin, baicalein, chrysin, 7,8-dihydroxyflavone, diosmin, flavoxate, 6-hydroxyflavone, luteolin, scutellarein, tangeritin and and/or scutellariae) and/or flavonols (e.g. amlensin, astragalin, azaleatin, azalein, fisetin, furanoflavonol galangin, gosypetin, 3-hydroxyflavone, hyperoside, icariin, isoquercetin, kaemperide, kaemperitrin, kaempferol, isorhamnetin, morin, myricetin, myricitrin, natudaidyne, pachypodol, pyranoflavonol quercetin, quercetin, rhamnagin, rhamnetin, robinin, rutin, spireoside, troxerutin and/or xanthoramnin).

In certain embodiments, compositions of the present disclosure comprise one or more flavanones. For example, according to certain embodiments, compositions of the present disclosure comprise butine, eriodictyol, hesperetin, hesperidin, homoeriodictyol, isosakuranetin, naringenin, naringin, pinocembrin, poncilin, sakuranetin, sakuranin and/or sterbin .

In certain embodiments, compositions of the present disclosure comprise one or more flavanonols. For example, according to certain embodiments, compositions of the present disclosure include dihydrokaempferol and/or taxifolin.

In certain embodiments, compositions of the present disclosure comprise one or more flavans. For example, in certain embodiments, the compositions of the present disclosure contain one or more flavan-3-ols (eg, catechin (C), catechin 3-gallate (Cg), epicatechin (EC), epigallocatechin ( EGC), epicatechin 3-gallate (ECg), epigallocatechin 3-gallate (EGCg), epiafzelekin, fisetinidol, gallocatechin 3-gallate (GC), gallocatechin 3-gallate (GCg), guibrutinidol, mesquitol, robinetinidol , theaflavin 3-gallate, theaflavin 3′-gallate, theaflavin 3,3′-digallate, thearubigin), flavan-4-ols (e.g. apiforol and/or luteofol) and/or flavan-3, 4-diol (e.g. leucocyanidin, leucodelphinidin, leucofisetinidin, leucomalvidin, leucopelargonidin, leucopeonidin, leucorobinetinidin, melakacidin and/or terakacidin) and/or dimers, trimers thereof, Including oligomers and/or polymers (eg, one or more proanthocyanidins).

In certain embodiments, compositions of the present disclosure comprise one or more isoflavonoids. For example, in certain embodiments, the inoculum composition of the present disclosure contains one or more isoflavones (e.g., biochanin A, daidzein, formononetin, genistein and/or glycitein), isoflavanes (e.g., equol, ionchocarpane). ) and/or laxifloorane), isoflavandiol, isoflavenes (e.g. glabrene, haginin D and/or 2-methoxyzdaicin), coumestans (e.g. coumestrol, plikadin and/or wederolactone), Contains pterocarpane and/or retinoids.

Compositions of the present disclosure include, but are not limited to, neoflavonoids (e.g., calophyllolides, ctareagenin, dalverdichromene, dalverdin, nivetin) and pterocarpanes (e.g., vitukarpine A, vitukarpine B, eribraedin A, eribraedin B, erytravicin II). , erythravicin-1, ericrystagalin, glycinol, glyceolysin, glyceoline, glycyrrhizol, marquiaine, medicarpine, molycyanin, orientanol, phaseolin, pisatine, striatin and/or trifolilysine). can contain.

Flavonoids and their derivatives can be incorporated into the compositions of the present disclosure in any suitable form, including but not limited to polymorphic and crystalline forms.

Compositions of the present disclosure include, but are not limited to, jasmonic acid ([1R-[1α,2β(Z)]]-3-oxo-2-(pentenyl)cyclopentaneacetic acid; JA), linoleic acid ((Z,Z )-9,12-octadecadienoic acid) and linolenic acid ((Z,Z,Z)-9,12,15-octadecatrienoic acid) and their analogs, derivatives, hydrates, isomers, polymers , salts and solvates, any suitable non-flavonoid nod gene inducer.

Jasmonic acid and its methyl ester methyl jasmonate (MeJA) (collectively referred to as jasmonates) are produced by several plants (e.g. wheat), fungi (e.g. Botryodiploidia theobromae), Gibrella An octadecanoid backbone naturally occurring in Gibbrella fujikuroi, yeasts (e.g. Saccharomyces cerevisiae) and bacteria (e.g. Escherichia coli) Linoleic acid and linolenic acid can be produced during the biosynthesis of jasmonic acid.Jasmonates, linoleic acid and linolenic acid (and their derivatives) are involved in nod gene expression or LCO production by rhizosphere bacteria. 44(11):759 (2006); Mabood et al., Agr. J. 98(2):289 (2006); 95(2-3):412 (2006); Mabood & Smith, "Linoleic and linolenic acid induce the expression of nod genes in Bradyrhizobium japonicum USDA 3", Plant Biol. (2001). Non-limiting examples of derivatives of jasmonic acid, linoleic acid, linolenic acid include esters, amides, glycosides and salts Representative esters are those in which the carboxyl group of linoleic acid, linolenic acid or jasmonic acid is converted to --COR wherein R is an --OR ¹ group and R ¹ is an alkyl group such as a C ₁ to C ₈ unbranched or branched alkyl group, for example methyl, ethyl or propyl groups; alkenyl groups such as C ₂ -C ₈ unbranched or branched alkenyl groups; alkynyl groups such as C ₂ -C ₈ unbranched or branched alkynyl groups; , an aryl group having from 6 to 10 carbon atoms; or a heteroaryl group having, for example, from 4 to 9 carbon atoms, wherein the hete R atoms can be, for example, N, O, P or S 2 . Representative amides are compounds in which the carboxyl group of linoleic acid, linolenic acid, or jasmonic acid is replaced with a ^--COR group, where R is a ^NR2R3 group ^, and ^R2 and R3 is independently hydrogen; alkyl groups such as C ₁ -C ₈ unbranched or branched alkyl groups, such as methyl, ethyl or propyl groups; C ₂ -C ₈ unbranched or branched alkenyl groups, such as linear alkenyl groups; alkynyl groups, such as C ₂ -C ₈ unbranched or branched alkynyl groups; aryl groups, for example having 6 to 10 carbon atoms; heteroaryl groups having 1 carbon atom, where the heteroatoms in the heteroaryl group can be N, O, P or S, for example. Esters can be prepared by known methods such as acid-catalyzed nucleophilic addition, in which a carboxylic acid is reacted with an alcohol in the presence of a catalytic amount of a mineral acid. Amides can be prepared by known methods, such as by reacting a carboxylic acid with a suitable amine under neutral conditions in the presence of a coupling agent such as dicyclohexylcarbodiimide (DCC). Suitable salts of linoleic acid, linolenic acid and jasmonic acid include, for example, base addition salts. Bases that can be used as reagents to prepare metabolically acceptable base salts of these compounds include alkali metal cations such as potassium and sodium and alkaline earth metal cations such as calcium and magnesium. and those derived from the cations of These salts can be readily prepared by mixing solutions of linoleic acid, linolenic acid or jasmonic acid together with solutions of the base. The salt may precipitate out of solution, be collected by filtration, or recovered by other means such as by evaporation of the solvent.

Compositions of the present disclosure include, but are not limited to, 2H-furo[2,3-c]pyran-2-one, and analogs, derivatives, hydrates, isomers, polymers, salts and solvates thereof. Any suitable karrikins having

In certain embodiments, the inoculum composition comprises one or more karrikin represented by formula LXXXIV:

(wherein Z is O, S or _NR5 ; _R1 , _{R2 ,} R3 and _R4 are each independently H, alkyl, alkenyl, alkynyl, phenyl, benzyl, hydroxy, hydroxyalkyl, alkoxy , phenyloxy, benzyloxy, CN, _COR6 , COOR=, halogen, _NR6R7 , or NO2 _{; R5} , _R6 and _R7 are _each independently H, alkyl or alkenyl), or a biologically acceptable salt thereof.

Non-limiting examples of biologically acceptable salts of karrikins include acid addition salts formed from biologically acceptable acids, examples of which include hydrochloride, hydrobromide, Sulfate or hydrogen sulfate, phosphate or hydrogen phosphate, acetate, benzoate, succinate, fumarate, maleate, lactate, citrate, tartrate, gluconate; methane Sulfonates, benzenesulfonates and p-toluenesulfonic acid are included. Additional biologically acceptable metal salts include alkali metal salts with bases, examples of which include sodium and potassium salts. Examples of compounds encompassed by Formula XXXX and which may be suitable for use in the present disclosure include 3-methyl-2H-furo[2,3-c]pyran-2- _one (wherein R = CH ₃ ; R ₂ , R ₃ , R ₄ = H), 2H-furo[2,3-c]pyran-2-one (wherein R ₁ , R ₂ , R ₃ , R ₄ = H ), 7-methyl-2H-furo[2,3-c]pyran-2-one (wherein R ₁ , R ₂ , R ₄ =H; R ₃ =CH ₃ ), 5-methyl-2H -furo[2,3-c]pyran-2-one (wherein R ₁ , R ₂ , R ₃ =H; R ₄ =CH ₃ ), 3,7-dimethyl-2H-furo[2, 3-c]pyran-2-one (wherein R ₁ , R ₃ =CH ₃ ; R ₂ , R ₄ =H), 3,5-dimethyl-2H-furo[2,3-c]pyran -2-one (wherein R ₁ , R ₄ =CH ₃ ; R ₂ , R ₃ =H), 3,5,7-trimethyl-2H-furo[2,3-c]pyran-2- one (wherein R ₁ , R ₃ , R ₄ =CH ₃ ; R ₂ =H), 5-methoxymethyl-3-methyl-2H-furo[2,3-c]pyran-2-one ( R ₁ =CH ₃ ; R ₂ , R ₃ =H; R ₄ =CH ₂ OCH ₃ ), 4-bromo-3,7-dimethyl-2H-furo[2,3-c]pyran- 2-one (wherein R ₁ , R ₃ =CH ₃ ; R ₂ =Br; R ₄ =H), 3-methylfuro[2,3-c]pyridin-2(3H)-one (wherein , Z=NH; R ₁ =CH ₃ ; R ₂ , R ₃ , R ₄ =H), and 3,6-dimethylfuro[2,3-c]pyridin-2(6H)-one (wherein Z=N-CH ₃ ; R ₁ =CH ₃ ; R ₂ , R ₃ , R ₄ =H). For example, U.S. Pat. No. 7,576,213; Halford, Smole Signal in CHEM. ENG. NEWS (April 12, 2010) (after forest fires, karrikin or butenolides in smoke act as growth stimulants, causing seed reported that it can promote germination and revitalize stored seeds such as corn, tomatoes, lettuce and onions).

Compositions of the present disclosure may comprise gluconolactone and/or one or more analogs, derivatives, hydrates, isomers, polymers, salts and/or solvates thereof.

Compositions of the present disclosure include, but are not limited to, dispersants, desiccants, antifreeze agents, seed fluidity improvers, herbicide safeners, anti-settling agents, pH buffers and adhesives. Any suitable excipient may be included.

Compositions of the present disclosure may include any suitable agriculturally acceptable dispersing agent including, but not limited to, surfactants and wetting agents. Selection of a suitable dispersant will depend on the intended use and the microorganisms present in the composition. Generally, dispersants will have low toxicity to the microorganisms in the composition and to the plant parts to which the composition is to be applied. In some embodiments, dispersants will be selected to wet and/or emulsify one or more soils. Non-limiting examples of dispersants include Atlox ^™ (e.g., 4916, 4991; Croda International PLC, Edison, NJ, USA), Atlox METASPERSE ^™ (Croda International PLC, Edison, NJ, USA). , BIO-SOFT® (e.g. N series, e.g. N1-3, N1-7, N1-5, N1-9, N23-3, N2.3-6.5, N25-3, N25-7, N25 -9, N91-2.5, N91-6, N91-8; Stepan Company, Northfield, Ill.), MAKON® nonionic surfactants (e.g., DA-4, DA-6 and DA-9; Stepan Company, Northfield, Ill.), MORWET® powder (Akzo Nobel Surface Chemistry LLC, Chicago, Ill.), MULTIWETTM surfactants (e.g. MO-85P-PW-(AP); Croda International PLC, Edison, NJ). ), SILWET® L-77 (Helena Chemical Company, Collierville, TN), SPAN ^™ surfactants (e.g., SPAN 20, 40, 60, 65, 80 and 85; Croda, Edison, NJ). ), TAMOL ^™ dispersants (Dow Chemical Company; Midland, Mich.), TERGITOL ^™ surfactants (e.g., TMN-6 and TMN-100X; Dow Chemical Company, Midland, Mich.), TERSPERSE interfaces. active agents (e.g. 2001, 2020, 2100, 2105, 2158, 2700, 4894 and 4896; Huntman Corp., Woodlands, TX), TRITON ^™ surfactants (e.g. X-100; The Dow Chemical Company, Midland, Mich.), TWEEN® surfactants (e.g., TWEEN® 20, 21, 22, 23, 28, 40, 60, 61, 65, 80, 81 and 85; Croda International PLC, New Jersey Edison) and combinations thereof. Additional examples of dispersants can be found in Baird & Zublena 1993. SOIL FACTS: Using Wetting Agents (Nonionic Surfactant) on Soil (North Carolina Co-oporative Extension Service Publication AG-439-25) (1993); Burges, "Formulations of Microbial Biopesticides: Benefical Microorganisms, Nematodes and Seed Treatments” (Springer Science & Business Media) (2012); McCarty, Wetting Agents (Clemson University Co-oporateive Extension Service Publication) (2001).

In some embodiments, compositions of the present disclosure comprise one or more anionic surfactants. According to some embodiments, the composition comprises one or more water-soluble anionic surfactants and/or one or more water-insoluble anionic surfactants and/or one or more water-insoluble anionic surfactants. Active agents, optionally alkyl carboxylates (e.g. sodium stearate), alkyl sulfates (e.g. alkyl lauryl sulfate, sodium lauryl sulfate), alkyl ether sulfates, alkylamide ether sulfates, alkylaryl polyethers Sulfate, Alkylarylsulfonate, Alkylarylsulfonate, Alkylsulfonate, Alkylamidesulfonate, Alkylarylsulfonate, Alkylbenzenesulfonate, Alkyldiphenyloxidesulfonate, α-Olefinsulfonate, Alkylnaphthalenesulfonate, Paraffinsulfonate, Alkylsulfosuccinate, Alkyl ether sulfosuccinate, alkylamide sulfosuccinate, alkyl sulfosuccinamate, alkyl sulfoacetate, alkyl phosphate, alkyl ether phosphate, acyl sarcosinate, acyl isethionate, N-acyl taurate, N-acyl- N-alkyl taurate, benzene sulfonate, cumene sulfonate, dioctyl sodium sulfosuccinate, ethoxylated sulfosuccinate, lignin sulfonate, linear alkyl benzene sulfonate, monoglyceride sulfate, perfluorobutane, perfluorooctane sulfonate, phosphate ester , styrene acrylic polymers, toluene sulfonates and xylene sulfonates.

In some embodiments, compositions of the present disclosure comprise one or more cationic surfactants. According to certain embodiments, the composition comprises one or more pH-dependent amines and/or one or more quaternary ammonium cations, optionally one or more cationic surfactants, wherein the alkyl Trimethylammonium salts (e.g. cetyltrimethylammonium bromide, cetyltrimethylammonium chloride), cetylpyridinium chloride, benzalkonium chloride, benzethonium chloride, 5-bromo-5-nitro-1,3-dioxane, dimethyldioctadecylammonium chloride, odorants selected from the group consisting of cetrimonium chloride, dioctadecyldimethylammonium bromide and/or octenidine dihydrochloride.

In some embodiments, compositions of the present disclosure comprise one or more nonionic surfactants. In some embodiments, the composition comprises one or more water-soluble nonionic surfactants and/or one or more water-insoluble nonionic surfactants, optionally one The above nonionic surfactants are alcohol ethoxylates (e.g. TERGITOL ^™ 15-S surfactants, e.g. TERGITOL ^™ 15-S-9 (The Dow Chemical Company, Midland, Mo.), alkanolamides, , alkanolamine condensates, carboxylic acid esters, cetostearyl alcohol, cetyl alcohol, cocamide DEA, dodecyldimethylamine oxide, ethanolamide, ethoxylates of glycerol and glycol esters, ethylene oxide polymers, ethylene oxide-propylene oxide copolymers, glucoside alkyl ethers, Glycerol alkyl ethers, glycerol esters, glycol alkyl ethers (e.g. polyoxyethylene glycol alkyl ethers, polyoxypropylene glycol alkyl ethers), glycol alkylphenol ethers (e.g. polyoxyethylene glycol alkylphenol ethers), glycol esters, monolaurin, pentaethylene glycol mono Dodecyl ether, poloxamer, polyamine, polyglycerol polylysine oleate, polypolysorbate, polyoxyethylenated fatty acid, polyoxyethylenated mercaptan, polyoxyethylenated polyoxypropylene glycol, polyoxyethylene glycol sorbitan alkyl ester, polyethylene glycol-polypropylene glycol selected from the group consisting of copolymers, polyoxyethylene glycol octylphenol ethers, polyvinylpyrrolidone, sugar-based alkylpolyglycosides, sulfanilamides, sorbitan fatty alcohol ethoxylates, sorbitan fatty acid ester ethoxylates, sorbitan fatty acid esters and/or tertiary acetylene glycols be done.

In some embodiments, compositions of the present disclosure comprise at least one nonionic surfactant. According to some embodiments, the compound comprises at least one water-insoluble nonionic surfactant and at least one water-soluble nonionic surfactant. In some embodiments, compositions of the present disclosure comprise a combination of nonionic surfactants having hydrocarbon chains of substantially the same length.

In some embodiments, compositions of the present disclosure comprise one or more zwitterionic surfactants. According to some embodiments, the composition comprises one or more betaines and/or one or more sultains, optionally 3-[(3-chloramidopropyl)dimethylammonio] - one or more zwitterionic surfactants selected from 1-propanesulfonate, cocamidopropyl betaine, cocamidopropyl hydroxysultaine, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine and/or one or more sphingomyelins include.

In some embodiments, compositions of the present disclosure comprise one or more soaps and/or organosilicone surfactants. According to some embodiments, the composition comprises one or more alkyl metal salts of fatty acids.

In some embodiments, compositions of the present disclosure comprise one or more humectants. According to some embodiments, one or more naphthalenesulfonic acids, optionally one or more alkylnaphthalenesulfonic acids (e.g. sodium alkylnaphthalenesulfonate), one or more isopropylnaphthalenesulfonic acids (e.g. , sodium isopropylnaphthalenesulfonate) and/or one or more butylnaphthalenesulfonic acids (eg, sodium n-butylnaphthalenesulfonate).

Compositions of the present disclosure include any suitable desiccant including, but not limited to, dry powders. Non-limiting examples of desiccants include AEROSIL® hydrophobic fumed silica powder (EVONIK Corporation, Piscataway, NJ), BENTOLITE® powder (BYK-Chemie GmbH, Wesel, Germany), INCOTEC ( ® powder (INCOTEC Inc., Salinas, Calif.), SIPERNAT® silica powder (Evonik Corporation, Parsippany, NJ) and mixtures thereof. Additional examples of desiccants can be found in BURGES, “Formulation of Microbial Biopesticides: Benefical Microorganisms, Nematodes and Seed Treatments” (Spring Science & Business Media) (2012). In some embodiments, the compositions of the present disclosure include calcium stearate, clay (e.g., attapulgite clay, montmorillonite clay), graphite, magnesium stearate, magnesium sulfate, milk powder, silica (e.g., fumed silica, hydrophobically coated silica, precipitated silica), soy lecithin and/or talc.

Compositions of the present disclosure may include any suitable cryoprotectant including, but not limited to, ethylene glycol, glycerin, propylene glycol and urea.

Compositions of the present disclosure may include an optional seed flowability agent to improve the lubricity of treated seeds. The flow improver may comprise one or more liquid lubricants, solid lubricants, liquid emulsions, or suspensions of solid lubricants. Non-limiting examples of flow improvers include lubricants such as fats and oils, natural and synthetic waxes, graphite, talc, fluoropolymers such as polytetrafluoroethylene, solid lubricants such as molybdenum disulfide and Tungsten disulfide may be mentioned. In some cases, flow improvers include wax materials. Non-limiting examples of wax materials that can be incorporated into liquid seed treatment compositions include waxes of plant and animal origin such as carnauba wax, candelilla wax, oliqury wax, beeswax, spermaceti, and paraffin wax. of petroleum-derived waxes. For example, optionally the flow improver includes carnauba wax. Optionally, flow improvers include oils. For example, the flow improver may include soybean oil. A non-limiting example of a commercially available wax material suitable for use as a flow improver is AQUAKLEAN 418, an anionic aqueous emulsion containing ultralight carnauba wax at 35% solids, supplied by Micro Powders, Inc. are mentioned.

Compositions of the present disclosure may include any suitable herbicide safener including, but not limited to, naphthalic anhydride.

Compositions of the present disclosure may include any suitable pH buffering agent including, but not limited to, monobasic potassium dihydrogen phosphate and dibasic potassium dihydrogen phosphate. In some embodiments, the composition has one pH selected to provide the composition with a pH of less than 10, typically from about 4.5 to about 9.5, from about 6 to about 8, or about 7. Contains pH buffering agents above.

The compositions of the present disclosure include, but are not limited to, polyvinyl acetate, polyvinyl alcohols with varying degrees of hydrolysis, polyvinylpyrrolidone, polyacrylates, water-soluble or dispersible acrylates, polyols or polyester-based pigment-based binders; Further two or more monomers such as acrylic acid, methacrylic acid, itaconic acid, maleic acid, fumaric acid, maleic anhydride, vinylpyrrolidone, ethylenically unsaturated monomers such as ethylene, butadiene, isoprene, chloroprene, styrene, divinyl. Benzene, ot-methylstyrene or p-methylstyrene, further vinyl halides such as copolymers of vinyl chloride and vinylidene chloride, further vinyl esters such as vinyl acetate, vinyl propionate or vinyl stearate, further vinyl methyl ketones or acrylics Esters of acids or methacrylic acids with monohydric alcohols or polyols, such as methyl acrylate, methyl methacrylate, ethyl acrylate, ethylene methacrylate, lauryl acrylate, lauryl methacrylate, decyl acrylate, N,N-dimethylaminoethyl methacrylate, 2-hydroxy Ethyl methacrylate, 2-hydroxypropyl methacrylate or glycidyl methacrylate, further diethyl esters or monoesters of unsaturated dicarboxylic acids, further (meth)acrylamide-N-methylol methyl ethers, amides or nitriles such as acrylamide, methacrylamide, N-methylol (meth)acrylamide, acrylonitrile, methacrylonitrile, as well as N-substituted maleimides and ethers such as vinyl butyl ether, vinyl isobutyl ether or vinyl phenyl ether, and combinations thereof. obtain.

Compositions of the present disclosure include, but are not limited to, one or more disaccharides (e.g., maltose), gums (e.g., cellulose gum, guar gum, gum arabic, sixing gum, xanthan gum), maltodextrins (e.g., one or more maltodextrins (e.g., maltodextrin (each and/or in total) having a DEV of about 10 to about 20), monosaccharides, oils (such as mineral oil, olive oil, peanut oil, soybean oil and/or sunflower oil) and/or oligosaccharides. may include any suitable adhesive.

Compositions of the present disclosure may contain any suitable pigment. Effect pigments, also sometimes referred to in the industry as "pearl pigments," are a class of raw materials that provide reflective, lustrous, and/or pearlescent effects when applied as a coating. In some cases, the effect pigment is in the form of a powder comprising a substrate raw material and a metal oxide coating. For example, effect pigments can include base materials including, but not limited to, talc, silicate materials (eg, mica), clay minerals, calcium carbonate, kaolin, phlogopite, alumina, and similar materials. In some cases, the base material comprises a hydrophilic material. The substrate raw material can be coated with a translucent layer of metal oxide including, but not limited to, titanium dioxide, iron oxide, chromium oxide, or zirconium oxide. Alternatively, in some cases the effect pigments comprise metal powders or metal flakes. Metal powders or metal flakes can include metals including, but not limited to, aluminum, copper, silver, or bronze. In some cases, the effect pigment comprises a silicate-based substrate. Non-limiting examples of particulate silicates that can be incorporated into the dry powder coating include mica coated with titanium dioxide (eg, SUNMICA FINE WHITE 2800102 available from Sun Chemical Corp.). Other non-limiting examples of commercially available effect pigments that can be incorporated into dry powders include MAGNA PEAL, LUMINA and MEARLIN pigments from BASF Corporation; PHIBRP PEARL from PhibroChem; and IRIDESSIUM 120 from Aakash Chemicals. be done. In some cases, the dry powder has an average particle size of about 1 to about 25 microns.

A composition of the present disclosure may comprise any suitable growth medium suitable for culturing one or more microorganisms in the composition. For example, in certain embodiments, compositions of the present disclosure comprise Czapek-Dox medium, glycerol yeast extract, mannitol yeast extract, potato glucose broth, and/or YEM medium.

Stabilizing compounds, biostimulants, microbial extracts, nutrients, pest attractants and/or feeding stimulants, pesticides, plant signaling molecules, dispersants, desiccants, herbicide safeners, flow improvers , anti-settling agents, buffers, adhesives, etc. can be incorporated into the compositions of the present disclosure in any suitable amount/concentration. The absolute value of the amount sufficient to cause the desired effect depends on the type, size and volume of material to which the composition is to be applied, the type of microorganisms in the composition, the number of microorganisms in the composition, the composition Factors such as the stability of the microorganisms therein, as well as the storage conditions (eg, temperature, relative humidity, duration) will influence. Those skilled in the art would know how to select effective amounts/concentrations/combinations using routine dose-response studies. Guidelines for selection of appropriate amounts/concentrations/combinations can be found, for example, in International Patent Publication Nos. WO2017/044473, WO2017/044545, WO2017/116837, WO2017/116846, WO2017/210163, WO2017/210166, WO2018/118740, WO2018/175681, WO2018/183491, WO2018/218008, WO2018/218016 and WO2018/218035.

In some embodiments, the compositions of the present disclosure comprise one or more carriers in an amount/concentration of about 1 to about 99% or more (by weight based on the total weight of the composition). For example, the compositions of the present disclosure can be , 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98 or 99% (by weight) of one or more non-aqueous carriers.

In some embodiments, the compositions of this disclosure comprise from about 0.0001 to about 95% or more (by weight based on the total weight of the composition) of one or more stabilizing compounds. For example, compositions of the present disclosure may contain from about 0.0001 to about 0.001, from about 0.001 to about 1%, from about 0.25% to about 5%, from about 1 to about 10%, from about 5 to about 25%, from about 10% to about 30%. %, about 20% to about 40%, about 25% to about 50%, about 30% to about 60%, about 50% to about 75%, or about 75% to about 95% (by weight), optionally to about 0.0005, 0.001, 0.002, 0.003, 0.004, 0.005, 0.0075, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% of one or more maltodextrins, monosaccharides, disaccharides, sugar alcohols, humic acid, betaine, proline, sarcosine, peptones, oxidation control ingredients, hygroscopic Contains polymers and/or UV protection agents.

In some embodiments, compositions of the present disclosure comprise one or more stabilizing compounds at a concentration of about ^1x10-20M to about ^1x10-1M . For example, the compositions of the present disclosure may contain 1×10 ⁻¹⁵ M to about 1×10 ⁻¹⁰ M, about 1×10 ⁻¹⁵ M to about 1×10 ⁻¹⁰ M, about 1×10 ⁻¹⁴ M to about 1 ×10 ^-8 M, about 1×10 ^-14 to about 1×10 ^-6 M, 1×10 ^-12 M to about 1×10 ^-8 M, about 1×10 ^-12 M to about 1×10 ^-6 M, about 1×10 ⁻¹⁰ M to about 1×10 ⁻⁶ M, about 1×10 ⁻⁸ to about 1×10 ⁻² M, optionally about 1×10 ⁻²⁰ M, about 1× 10 ^-19 M, about 1 x 10 ^-18 M, about 1 x 10 ^-17 M, about 1 x 10 ^-16 M, about 1 x 10 ^-15 M, 1 x 10 ^-14 M, 1 x 10 ^-13 M , 1 x 10 ^-12 M, 1 x 10 ^-11 M, 1 x 10 ^-10 M, 1 x 10 ^-9 M, 1 x 10 ^-8 M, 1 x 10 ^-7 M, 1 x 10 ^-6 M, 1 x 10 ^-5 M, 1 x 10 ^-4 M, 1 x 10 ^-3 M, 1 x 10 ^-2 M, 1 x 10 ^-1 M or more of one or more maltodextrins, monosaccharides, disaccharides, sugars It may contain alcohols, humic acids, betaine, proline, sarcosine, peptones, oxidation control ingredients, hygroscopic polymers and/or UV protectants.

In some embodiments, compositions of the present disclosure comprise one or more monosaccharides in an amount/concentration of about 0.005% to about 50% (by weight) of the composition. For example, the compositions of the present disclosure may contain about/at least/less than about/at least/less than , 0.75, 1, 1.25, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 10, 15, 20, 25% (by weight) ) such as arabinose, fructose and/or glucose. In some embodiments, it is present at a concentration ranging from about 1 x ^10-20M to about 1 x ^10-1M . For ^example , the ^{one or more disaccharides are} M, 1 x 10 ^-14 M, 1 x 10 ^-13 M, 1 x 10 ^-12 M, 1 x 10 ^-11 M, 1 x 10 ^-10 M.

In some embodiments, compositions of the present disclosure comprise one or more disaccharides in an amount/concentration of about 0.005 to about 50% or more (by weight) of the composition. For example, the compositions of the present disclosure may contain about/at least/less than about/at least/less than , 0.75, 1, 1.25, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 10, 15, 20, 25% (by weight) ) (eg, maltose, sucrose and/or trehalose). In some embodiments, one or more disaccharides are present at a concentration ranging from about 1 x ^10-20M to about 1 x ^10-1M . For example, the one or more disaccharides are about/at least/less than 1×10 ⁻²⁰ M, 1×10 ⁻¹⁹ M, 1×10 ⁻¹⁸ M, 1×10 ⁻¹⁷ M, 1×10 ⁻¹⁶ M , 1×10 ⁻¹⁵ M, 1×10 ⁻¹⁴ M, 1×10 ⁻¹³ M, 1×10 ⁻¹² M, 1×10 ⁻¹¹ M, 1×10 ⁻¹⁰ M.

In some embodiments, compositions of the present disclosure comprise one or more maltodextrins in an amount/concentration of about 0.001 to about 95% or more (by weight). In some embodiments, the maltodextrin comprises one or more maltodextrin in an amount/concentration of about 0.001 to about 95% or more (by weight). In some embodiments, maltodextrin is about 0.01% to about 1%, about 0.25% to about 5%, about 1% to about 10%, about 5% to about 25%, about 10% to about 30%, from about 20% to about 40%, from about 25% to about 50%, from about 50% to about 75%, or from about 75% to about 95% (by weight). For example, compositions of the present disclosure may contain one or more maltodextrins (eg, one or more maltodextrins (each and/or collectively) of about/at least/less than 0.01, 0.02 having a DEV value of about 15-20. , 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7 , 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (weight in ).

In some embodiments, the compositions of the present disclosure comprise one or more sugar alcohols in amounts/concentrations from about 0.001 to about 95% or more (by weight). In some embodiments, the sugar alcohol (eg, arabitol, mannitol, sorbitol and/or xylitol) is about 0.001% to about 1%, about 0.25% to about 5%, about 1% to about 10%, about 5% to about 5% of the composition. comprising about 25%, about 10% to about 30%, about 20% to about 40%, about 25% to about 50%, about 50% to about 75%, or about 75% to about 95% (by weight) . For example, compositions of the present disclosure may contain one or more sugar alcohols (eg, arabitol, mannitol, sorbitol and/or xylitol) at about/at least/less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight).

In some embodiments, compositions of the present disclosure comprise one or more humic acids in an amount/concentration of about 0.001 to about 95% or more (by weight) of the composition. In some embodiments, humic acid (eg, potassium humate) is about 0.001 to about 1%, about 0.25 to about 5%, about 1 to about 10%, about 5 to about 25%, about 10% of the composition. % to about 30%, about 20% to about 40%, about 25% to about 50%, about 50% to about 75%, or about 75% to about 95% (by weight). For example, compositions of the present disclosure may contain one or more humic acids (eg, potassium humate and/or sodium humate) at about/at least/less than 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight).

In some embodiments, the compositions of the present disclosure comprise one or more UV protectants in an amount/concentration (by weight) of about 0.0001 to about 5% or more of the composition. In some embodiments, the UV protectant (eg, calcium lignosulfonate, sodium lignosulfonate) is about 0.0001 to about 0.001, about 0.001 to about 1%, about 0.25% to about 5% or more (by weight) of the composition. in) amount/concentration. For example, the compositions of the present disclosure may contain about/at least/less than , 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.75, 1, 1.25, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5% or more (by weight) of one or more UV protectants (eg calcium lignosulfonate and/or sodium lignosulfonate).

In some embodiments, the compositions of the present disclosure comprise one or more antioxidant ingredients in an amount/concentration (by weight) of about 0.0001 to about 5% or more of the composition. For example, the compositions of the present disclosure may contain about/at least/less than , 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.75, 1, 1.25, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5% of one or more antioxidant components. In some embodiments, the amount/concentration of the antioxidant ingredient is from about 0.005 to about 2% (by weight) of the composition. In some embodiments, the oxidation inhibitor component comprises one or more oxidation inhibitor components at concentrations ranging from about 1×10 ⁻²⁰ M to about 1×10 ⁻¹ M. For example, the one or more oxidation inhibiting components may be about/at least/less than 1×10 ⁻²⁰ M, 1×10 ⁻¹⁹ M, 1×10 ⁻¹⁸ M, 1×10 ⁻¹⁷ M, 1×10 ⁻¹⁶ M, 1×10 ^-15 M, 1×10 ^-14 M, 1×10 ^-13 M, 1×10 ^-12 M, 1×10 ^-11 M, 1×10 ^-10 M can. In some embodiments, compositions of the present disclosure comprise one or more commercially available antioxidants used according to the manufacturer's recommended amount/concentration. In some embodiments, compositions of the present disclosure comprise one or more commercially available oxygen scavengers used according to the manufacturer's recommended amount/concentration.

In some embodiments, the compositions of this disclosure are 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0 , 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or higher relative humidity, 1, 2 , 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36 , 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer storage; about 5, 10, 15 , 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more dry; about 5, 10, 15, 20, 25 , 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more dry and 0, 1, 2, 3, 4, 5, 6 , 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31 , 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 at 70, 75, 80, 85, 90, 95% or more relative humidity , 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92 , 96, 100, 104 weeks or more; , 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88 , cryopreservation for periods of 92, 96, 100, 104 weeks or longer; plant propagation material (optionally seeds) application to plant propagation material and about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90%, 95 dry up to 0% or more; , 18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39 and/or 40°C and 1, 2 at 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity , 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36 , 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer storage; foliar application; Spraying and drying to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; and/ or foliar spray plus 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22 , 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0, 5, 10, 15, 0.1, 0.2, 0.25, 0.5, 0.75 to relative humidity of 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 days or one or more stabilizing compounds in amounts/concentrations sufficient to ensure that the one or more strains of the present disclosure remain viable after exposure for a period of time or longer.

In some embodiments, the compositions of this disclosure are 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0 , 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or higher relative humidity, 1, 2 , 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36 , 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer storage; about 5, 10, 15 , 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more dry; about 5, 10, 15, 20, 25 , 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more dry and 0, 1, 2, 3, 4, 5, 6 , 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31 , 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 at 70, 75, 80, 85, 90, 95% or more relative humidity , 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92 , 96, 100, 104 weeks or more; , 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88 , cryopreservation for periods of 92, 96, 100, 104 weeks or longer; plant propagation material (optionally seeds) application to plant propagation material and about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% drying to or more; 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0 , 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, storage for 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer; foliar application; foliar application and about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more dry; and/or foliar spray and 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, Temperatures 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0, 5, 10, 15, 20 , 0.1, 0.2, 0.25, 0.5, 0.75, 1 to 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity ,1.5,2,2.5,3,3.5,4,4.5,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21 days or At least 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45 of the strains of the present disclosure after exposure for a longer period of time , 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% contain one or more stabilizing compounds in amounts/concentrations sufficient to ensure viability.

In some embodiments, the compositions of this disclosure are 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0 , 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or higher relative humidity, 1, 2 , 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36 , 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer storage; about 5, 10, 15 , 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more dry; about 5, 10, 15, 20, 25 , 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more dry and 0, 1, 2, 3, 4, 5, 6 , 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31 , 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 at 70, 75, 80, 85, 90, 95% or more relative humidity , 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92 , 96, 100, 104 weeks or more; , 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88 , cryopreservation for periods of 92, 96, 100, 104 weeks or longer; plant propagation material (optionally seeds ); application to plant propagation material and about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90%, Drying up to 95% or more; 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 1 at relative humidity of 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, Storage for 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer; foliar application; surface application and drying to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; and /or foliar application and 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0, 5, 10, 15 , 0.1, 0.2, 0.25, 0.5, 0.75 to 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity , 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 At least 1×10 ¹ , 1×10 ² , 1×10 ³ , 1×10 ⁴ , 1×10 strains of the present disclosure per gram and/or milliliter of composition after exposure for a period of days or more Sufficient amount/concentration to ensure viability of ⁵ , 1 x ¹⁰⁶ , 1 x ¹⁰⁷ , 1 x ¹⁰⁸ , 1 x 109, ¹ x ¹⁰¹⁰ or more colony forming units (cfu) with one or more stabilization including compounds.

In some embodiments, the compositions of the present disclosure are stored at temperatures at which the compositions should be stored (e.g., 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 , 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38 , 39 and/or 40°C) and the composition has a deliquescent relative humidity (DRH) of 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75 , 80, 85 or 90, containing one or more stabilizing compounds.

In some embodiments, compositions of the disclosure comprise two or more stabilizing compounds that synergistically enhance the stability and/or maintenance of viability of the sclerotia of the disclosure.

Stabilizing compounds can be incorporated into the compositions of the present disclosure in any suitable proportion.

In some embodiments, the compositions of this disclosure are 50:50, 55:45, 60:40, 65:35, 70:30, 75:25, 80:20, 85:15, 90:10, 95:5 maltodextrins: (monosaccharides, disaccharides, sugar alcohol and/or humic acid) ratio of one or more maltodextrins and one or more monosaccharides, disaccharides, sugar alcohols and/or humic acids. For example, the compositions of the present disclosure contain one or more maltodextrins (eg, one or more maltodextrins having a DEV (each and/or in total) of about 15 to about 20) and one or more sugar alcohols (e.g. sorbitol and xylitol) and/or humic acid (e.g. potassium humate) to about 5:95, about 15:85, about 25:75, or about 50:50 maltodextrin: (sugar alcohol/humic acid) Can be included in ratios.

In some embodiments, compositions of the present disclosure comprise one or more biostimulants in an amount/concentration of about 0.0001 to about 5% or more (by weight) of the composition. In some embodiments, the biostimulant (e.g., glycine and/or seaweed extract) is about 0.0025, 0.003, 0.0035, 0.0045, 0.0045, 0.0055, 0.0055, 0.0065, 0.0065, 0.0075, 0.0085, 0.0085, 0.0095, 0.0095, 0.015, 0.025, 0.025 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1 to about 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1 , 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6 , 4.7, 4.8, 4.9, 5% (by weight). For example, the compositions of the present disclosure can have a 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5% or more (by weight) of one or more biostimulants (eg glycine and/or seaweed extract).

In some embodiments, compositions of the present disclosure comprise one or more microbial extracts in an amount/concentration of about 0.0001 to about 5% or more (by weight) of the composition. In some embodiments the microbial extract is about 0.005, 0.0055, 0.006, 0.0065, 0.007, 0.0075, 0.008, 0.0085, 0.009, 0.0095, 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.06, 0.06, 0.07, 0.06, 0.07, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1 to about 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6 , 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5% ( by weight). For example, the compositions of the present disclosure can have a 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5% or more (by weight) of one or more microbial extracts.

In some embodiments, compositions of the present disclosure comprise one or more nutrients in an amount/concentration of about 0.0001 to about 5% or more (by weight) of the composition. In some embodiments, nutrients (eg, phosphorus, boron, chlorine, copper, iron, manganese, molybdenum and/or zinc) are about 0.0001, 0.0002, 0.0003, 0.0004, 0.0005, 0.0006, 0.0007, 0.0008, 0.0009, 0.001, 0.0015, 0.002, 0.0025, 0.0035, 0.0035, 0.0045, 0.0045, 0.0055, 0.0065, 0.0065, 0.0075, 0.0075, 0.0085, 0.0095, 0.0095 0.04, 0.045, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1 to about 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7 , 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2 , 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5% (by weight). For example, the compositions of the present disclosure can have a 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5% or more (by weight) of one or more nutrients such as phosphorus, boron, chlorine, copper, iron, manganese, molybdenum and/or zinc ).

In some embodiments, compositions of the present disclosure comprise one or more pest attractants and/or feeding stimulants in amounts/concentrations of about 0.0001 to about 5% or more of the composition. In some embodiments, the pest attractant and/or feeding stimulant is at about 0.003, 0.0035, 0.0035, 0.0045, 0.0045, 0.0055, 0.0065, 0.0065, 0.0075, 0.0075, 0.0085, 0.0095, 0.0095, 0.009 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1 to about 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2 , 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7 , accounting for 4.8, 4.9 and 5% (by weight). For example, the compositions of the present disclosure can have a 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5% or more (by weight) of one or more pest attractants and/or feeding stimulants.

In some embodiments, the compositions of the present disclosure contain one or more LCOs from about 1×10 ⁻¹⁵ M to about 1×10 ⁻¹⁰ M, from about 1×10 ⁻¹⁴ M to about 1×10 ⁻⁸ M, about 1×10 ^-14 M to 1×10 ^-6 , about 1×10 ^-12 M to about 1×10 ^-8 M, about 1×10 ^-12 M to about 1×10 ^-6 M, about 1 ^x10-10 M to about 1 x ^10-6 M, or about 1 x ^10-8 M to about 1 x ^10-2 M. For example, the compositions of the present disclosure can be about 1×10 ⁻²⁰ M, about 1×10 ⁻¹⁹ M, about 1×10 ⁻¹⁸ M, about 1×10 ⁻¹⁷ M, about 1×10 ⁻¹⁶ M, about 1×10 ^-15 M, about 1×10 ^-14 M, about 1×10 ^-13 M, about 1×10 ^-12 M, about 1×10 ^-11 M, about 1×10 ^-10 M, about 1× 10 ^-9 M, about 1 x 10 ^-8 M, about 1 x 10 ^-7 M, about 1 x 10 ^-6 M, about 1 x 10 ^-5 M, about 1 x 10 ^-4 M, about 1 x 10 ^{- 3} , about 1×10 ⁻² M, including one or more LCOs of 1×10 ⁻¹ M or more (eg, 1, 2, 3, 4 or more LCOs shown as Structures V to XXXIII) .

The compositions of the present disclosure contain one or more chitin oligomers from about 1×10 ⁻¹⁵ M to about 1×10 ⁻¹⁰ M, from about 1×10 ⁻¹⁴ M to about 1×10 ⁻⁸ M, from about 1×10 ^-14 M to 1×10 ^-6 , 1×10 ^-12 M to 1×10 ^-8 M, 1×10 ^-12 M to 1×10 ^-6 M, 1×10 ^-10 M to Containing at a concentration of about 1×10 ^-6 M, or about 1×10 ^-8 M to about 1×10 ^-2 M. For example, the compositions of the present disclosure can be about 1×10 ⁻²⁰ M, about 1×10 ⁻¹⁹ M, about 1×10 ⁻¹⁸ M, about 1×10 ⁻¹⁷ M, about 1×10 ⁻¹⁶ M, about 1×10 ^-15 M, about 1×10 ^-14 M, about 1×10 ^-13 M, about 1×10 ^-12 M, about 1×10 ^-11 M, about 1×10 ^-10 M, about 1× 10 ^-9 M, about 1 x 10 ^-8 M, about 1 x 10 ^-7 M, about 1 x 10 ^-6 M, about 1 x 10 ^-5 M, about 1 x 10 ^-4 M, about 1 x 10 ^{- 3} , about 1×10 ⁻² M, 1×10 ⁻¹ M or more of one or more chitin oligomers (eg, 1, 2, 3, 4 or more oligosaccharides of chitin oligomers shown as structures XXXVI-LXXXIII) )including.

The compositions of the present disclosure contain one or more chitosan oligomers from about 1 x 10 ^-15 M to about 1 x 10 ^-10 M, from about 1 x 10 ^-14 M to about 1 x 10 ^-8 M, about 1 x 10 ^-14 M to 1×10 ^-6 , about 1×10 ^-12 M to about 1×10 ^-8 M, about 1×10 ^-12 M to about 1×10 ^-6 M, about 1×10 ^-10 M to about 1×10 ^-6 M, or from about 1×10 ^-8 M to about 1×10 ^-2 M. For example, the compositions of the present disclosure can be about 1×10 ⁻²⁰ M, about 1×10 ⁻¹⁹ M, about 1×10 ⁻¹⁸ M, about 1×10 ⁻¹⁷ M, about 1×10 ⁻¹⁶ M, about 1×10 ^-15 M, about 1×10 ^-14 M, about 1×10 ^-13 M, about 1×10 ^-12 M, about 1×10 ^-11 M, about 1×10 ^-10 M, about 1× 10 ^-9 M, about 1 x 10 ^-8 M, about 1 x 10 ^-7 M, about 1 x 10 ^-6 M, about 1 x 10 ^-5 M, about 1 x 10 ^-4 M, about 1 x 10 ^{- 3} , including one or more chitosan oligomers (eg, 1, 2, 3, 4 or more oligosaccharides in deacetylated form) of about 1×10 ^-2 M, 1×10 ^-1 M or more.

In some embodiments, the compositions of the present disclosure contain one or more chitin from about 1×10 ⁻¹⁵ M to about 1×10 ⁻¹⁰ M, from about 1×10 ⁻¹⁴ M to about 1×10 ⁻⁸ M, about 1×10 ^-14 M to 1×10 ^-6 , about 1×10 ^-12 M to about 1×10 ^-8 M, about 1×10 ^-12 M to about 1×10 ^-6 M, about 1 ^x10-10 M to about 1 x ^10-6 M, or about 1 x ^10-8 M to about 1 x ^10-2 M. For example, the compositions of the present disclosure can be about 1×10 ⁻²⁰ M, about 1×10 ⁻¹⁹ M, about 1×10 ⁻¹⁸ M, about 1×10 ⁻¹⁷ M, about 1×10 ⁻¹⁶ M, about 1×10 ^-15 M, about 1×10 ^-14 M, about 1×10 ^-13 M, about 1×10 ^-12 M, about 1×10 ^-11 M, about 1×10 ^-10 M, about 1× 10 ^-9 M, about 1 x 10 ^-8 M, about 1 x 10 ^-7 M, about 1 x 10 ^-6 M, about 1 x 10 ^-5 M, about 1 x 10 ^-4 M, about 1 x 10 ^{- 3} , contains one or more chitins of about 1×10 ^-2 M, 1×10 ^-1 M or more.

In some embodiments, the compositions of the present disclosure contain one or more chitosans from about 1×10 ⁻¹⁵ M to about 1×10 ⁻¹⁰ M, from about 1×10 ⁻¹⁴ M to about 1×10 ⁻⁸ M, about 1×10 ^-14 M to 1×10 ^-6 , about 1×10 ^-12 M to about 1×10 ^-8 M, about 1×10 ^-12 M to about 1×10 ^-6 M, about 1 ^x10-10 M to about 1 x ^10-6 M, or about 1 x ^10-8 M to about 1 x ^10-2 M. For example, the compositions of the present disclosure can be about 1×10 ⁻²⁰ M, about 1×10 ⁻¹⁹ M, about 1×10 ⁻¹⁸ M, about 1×10 ⁻¹⁷ M, about 1×10 ⁻¹⁶ M, about 1×10 ^-15 M, about 1×10 ^-14 M, about 1×10 ^-13 M, about 1×10 ^-12 M, about 1×10 ^-11 M, about 1×10 ^-10 M, about 1× 10 ^-9 M, about 1 x 10 ^-8 M, about 1 x 10 ^-7 M, about 1 x 10 ^-6 M, about 1 x 10 ^-5 M, about 1 x 10 ^-4 M, about 1 x 10 ^{- 3} , containing one or more chitosans at a concentration of about 1×10 ^-2 M, 1×10 ^-1 M or greater.

In some embodiments, compositions of the present disclosure comprise one or more dispersants in an amount/concentration of about 0.001 to about 25% or more (by weight) of the composition. In some embodiments, the dispersant is about 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.004, 0.0045, 0.005, 0.0055, 0.006, 0.0065, 0.007, 0.0075, 0.008, 0.0085, 0.009, 0.0095 of the composition. , 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2 , 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7 , 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 6, 7, 8, 9 or 10 to about 1, 1.5, 2, 2.5, 3, 3.5, up to 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20% by weight ). For example, the compositions of the present disclosure can have a 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20% or more (by weight) of one or more dispersants, such as one or more surfactant and/or dispersant).

In some embodiments, the compositions of the present disclosure comprise one or more desiccant in an amount/concentration of about 0.001 to about 25% or more (by weight) of the composition. In some embodiments, the dispersant is about 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.004, 0.0045, 0.005, 0.0055, 0.006, 0.0065, 0.007, 0.0075, 0.008, 0.0085, 0.009, 0.0095 of the composition. , 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2 , 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7 , 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 6, 7, 8, 9 or 10 to about 1, 1.5, 2, 2.5, 3, 3.5, up to 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20% by weight ). For example, the compositions of the present disclosure can have a 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, It may contain 75, 80, 85, 90, 95% or more (by weight) of one or more desiccants such as lecithin and/or talc.

In some embodiments, compositions of the present disclosure comprise from about 0.5 to about 10 grams of dry powder per liter of composition. For example, the compositions of the present disclosure contain about 0.5, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5 per liter of composition. , 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10 grams or more of dry powder.

In some embodiments, compositions of the present disclosure comprise one or more buffering agents in an amount/concentration of about 0.0001 to about 5% or more (by weight) of the composition. In some embodiments, the buffering agent is about 0.0001, 0.0002, 0.0003, 0.0004, 0.0005, 0.0006, 0.0007, 0.0008, 0.0009, 0.001, 0.0015, 0.002, 0.0025, 0.003, 0.0035, 0.005, 0.0004. . , 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1 to about 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5% by weight at). For example, the compositions of the present disclosure can have a 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5% or more (by weight) of one or more buffering agents such as monobasic potassium phosphate (monopotassium phosphate) and/or dibasic potassium phosphate (dipotassium phosphate)).

In some embodiments, the compositions of the present disclosure contain one or more commercially available carriers, antioxidants, hygroscopic polymers, UV protectants, bioirritants, used according to the manufacturer's recommended amounts/concentrations. , microbial extracts, nutrients, pest attractants and/or feeding stimulants, pesticides, plant signal molecules, dispersants, desiccants, antifreeze agents, buffers and/or adhesives.

In some embodiments, the strain of the disclosure is the only strain in the composition of the disclosure.

Some embodiments comprise one or more microorganisms in addition to the strains of the present disclosure. Any suitable microorganism can be added to agriculturally beneficial microorganisms including, but not limited to, diazotrophs (e.g., commensal diazotrophs), phosphate-solubilizing microorganisms, mycorrhizal fungi, and biopesticides. can. In some embodiments, compositions of the present disclosure comprise one or more microorganisms selected from the genera and species listed in Appendix A. Selection of additional microorganisms (if any) will depend on the intended use.

Non-limiting examples of bacteria that can be included in the compositions of the present disclosure include Azospirillum brasilense INTA Az-39, Bacillus amyloliquefaciens D747, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) NRRL B-50349, Bacillus amyloliquefaciens TJ1000, Bacillus amyloliquefaciens FZB24, Bacillus amyloliquefaciens FZB42, Bacillus amyloliquefaciens Bacillus amyloliquefaciens IN937a, Bacillus amyloliquefaciens IT-45, Bacillus amyloliquefaciens TJ1000, Bacillus amyloliquefaciens MB1600, Bacillus amyloliquefaciens Bacillus amyloliquefaciens BS27 (deposited as NRRLB-5015), Bacillus amyloliquefaciens BS2084 (deposited as NRRLB-5013), Bacillus amyloliquefaciens 15AP4 (ATCC PTA-6507), Bacillus amyloliquefaciens 3AP4 (deposited as ATCC PTA-6506), Bacillus amyloliquefaciens LSSA01 (deposited as NRRL B-50104). Bacillus amyloliquefaciens ABP278 (deposited as NRRL B-50634), Bacillus amyloliquefaciens Bacillus amyloliquefaciens 1013 (deposited as NRRL B-50509), Bacillus amyloliquefaciens 918 (deposited as NRRL B-50508), Bacillus amyloliquefaciens 22CP1 (ATCC PTA) -6508), and Bacillus amyloliquefaciens BS18 (deposited as NRRL B-50633), Bacillus cereus (Bacillus cereus) I-1562, Bacillus firmus (Bacillus firmus) I-1582, Bacillus liqueniformis (Licheniformis bacillus) BA842 (deposited as NRRL B-50516), Bacillus liqueniformis BL21 (deposited as NRRL B-50134), Bacillus mycoides NRRL B-21664, Bacillus pumilus NRRL B-21662, Bacillus pumilus NRRL B-30087, Bacillus pumilus ATCC 55608, Bacillus pumilus ATCC 55609, Bacillus pumilus GB34 Bacillus pumilus KFP9F, Bacillus pumilus QST 2808, Bacillus subtilis (Bacillus subtilis) ATCC 55078, Bacillus subtilis ATCC 55079, Bacillus subtilis MBI600, Bacillus subtilis NRRL B-21661, Bacillus subtilis NRRL B-21665, Bacillus subtilis CX-9060, Bacillus subtilis (Bacillus subtilis) GB03, Bacillus subtilis GB07, Bacillus subtilis QST-713, Bacillus subtilis FZB24, Bacillus subtilis D747, Bacillus subtilis ) 3BP5 (deposited as NRRL B-50510), Bacillus thringiensis ATCC 13367, Bacillus thringiensis GC-91, Bacillus thringiensis NRRL B-21619, Bacillus - Bacillus thringiensis ABTS-1857, Bacillus thringiensis SAN 401 I, Bacillus thringiensis ABG-6305, Bacillus thringiensis ABG-6346, Bacillus thuringiensis Bacillus thringiensis AM65-52, Bacillus thringiensis SA-12, Bacillus thringiensis SB4, Bacillus thringiensis ABTS-351, Bacillus thringiensis ) HD-1, Bacillus thringiensis EG 2348, Bacillus thringiensis EG 7826, Bacillus thringiensis EG 7841, Bacillus thringiensis DSM 2803, Bacillus thringiensis・Bacillus thringiensis NB-125, Bacillus thuringiensis (B acillus thringiensis NB-176, BRADY, Mesorhizobium huakii LL32, Pseudomonas jessenii PS06, Rhizobium leguminosarum 162BB1, Rhizobium leguminosarum 162P17 Rhizobium leguminosarum 175G10b, Rhizobium leguminosarum D36, Rhizobium leguminosarum SO12A-2 (IDAC 080305-01), Rhizobium loti 95C11, Rhizobium loti 14, Rhizobium nori loti 95Cフレディ（Sinorhizobium fredii）CCBAU114、シノリゾビウム・フレディ（Sinorhizobium fredii）USDA 205、シノリゾビウム・メリロチ（Sinorhizobium meliloti）102F34a、シノリゾビウム・メリロチ（Sinorhizobium meliloti）102F51a、シノリゾビウム・メリロチ（Sinorhizobium meliloti）102F77b、シノリゾビウム・メリロチ（Sinorhizobium meliloti ) B401, Yersinia entomophaga O82KB8, and combinations thereof, and at least 75, 80, 85, 90, 95, 96, 97, 97.5, 98 with any one of the above strains based on 16S rDNA sequence identity , 98.5, 99, 99.5, 99.6, 99.7, 99.8, 99.9% or greater identity.

Non-limiting examples of fungi that can be included in the compositions of the present disclosure include Gliocladium virens ATCC 52045, Gliocladium virens GL-21, Glomus intraradices (Glomus intraradices) RTI-801, Metarhizium anisopliae F52, PENI, Trichoderma asperellum SKT-1, Trichoderma asperellum ICC 012, Trichoderma atroviride LC52, Trichoderma - Trichoderma atroviride CNCM 1-1237, Trichoderma fertile JM41R, Trichoderma gamsii ICC 080, Trichoderma hamatum ATCC 52198, Trichoderma harzianum ATCC 52445 Trichoderma harzianum KRL-AG2, Trichoderma harzianum T-22, Trichoderma harzianum TH-35, Trichoderma harzianum T-39, Trichoderma harzianum ) ICC012, Trichoderma reesei ATCC 28217, Trichoderma virens ATCC 58678, Trichoderma virens GI-3, Trichoderma virens GL-21, Trichoderma virens virens) G-41, Trichoderma viridae ATCC 52440, Trichoderma viridae Trichoderma viridae ICC080, and/or Trichoderma viridae TV1 and combinations thereof, and any of the above strains based on internal transcribed spacer (ITS) and/or cytochrome c oxidase (COI) sequence identity microorganisms having at least 75, 80, 85, 90, 95, 96, 97, 97.5, 98, 98.5, 99, 99.5, 99.6, 99.7, 99.8, 99.9% or more identity with one or more.

Non-limiting examples of mycorrhizal fungi that can be included in the compositions of the present disclosure include Gigaspora margarita, Glomus aggregatum, Glomus brasilianum, Glomus klarum ( Glomus clarum, Glomus deserticola, Glomus etunicatum, Glomus intraradices, Glomus monosporum, Glomus mosseae, Rakkaria bicolor ( Laccaria bicolor), Laccaria laccata, Paraglomus brazilianum, Pisolithus tinctorius, Rhizopogon amylopogon, Rhizopogon fulvigleha, Rhizopogon luteolus ( mycorrhizal fungal strains such as Rhizopogon luteolus, Rhizopogon virosuli, Scleroderma cepa and Scleroderma citrinum and combinations thereof.

Examples of additional microorganisms that can be added to the compositions of the present disclosure can be found in Appendix A.

Additional microorganisms can be incorporated into the inoculum composition of the present disclosure in any suitable amount/concentration. The absolute amount/concentration sufficient to cause the desired effect depends on the type, size and volume of material to which the composition will be applied, the type of microorganisms in the composition, the number of microorganisms in the composition, the composition It can be influenced by factors such as the stability of the microorganisms in the article as well as storage conditions (eg, temperature, relative humidity, duration). Those of ordinary skill in the art will understand how to select effective amounts/concentrations using routine dose-response experiments. Guidance for the selection of appropriate amounts/concentrations can be found, for example, in 027821、WO2017/044473、WO2017/044545、WO2017/077104、WO2017/083623、WO2017/116837、WO2017/116846、WO2017/131971、WO2017/205258、WO2017/210163、WO2017/210166、WO2018/118740、WO2018/129016、 WO2018/129018, WO2018/175677, WO2018/175681, WO2018/183491, WO2018/218008, WO2018/218016 and WO2018/218035 pamphlets, and U.S. Patent Publication Nos. 2006/258534, 2010592 and 2010592 and 2012345 You can see it in the description.

In some embodiments, the one or more additional microorganisms fix atmospheric nitrogen, solubilize phosphate, and one or more of It is present (eg, in the soil) in an effective amount/concentration to control phytopathogenic pests, increase stress tolerance, and/or improve plant growth/yield.

In some embodiments, the one or more additional microorganisms fix atmospheric nitrogen, solubilize phosphate, and one or more phytopathogenic microbes when the composition is applied to a plant or plant part. It is present in an amount/concentration effective to control pests, increase stress tolerance, and/or improve plant growth/yield.

In some embodiments, the one or more additional microorganisms are present in an amount ranging from about 1 x 101 to about ¹ x ¹⁰¹² colony forming units (cfu) per gram and/or milliliter of the composition. . According to certain embodiments, about 1 x 101, 1 x 102, 1 x 103, 1 x ¹⁰⁴ , 1 x 105, ¹ x ¹⁰⁶ , ¹ x ^{10 per} gram and/or milliliter of composition. ⁷ , 1×10 ⁸ , 1×10 ⁹ , 1×10 ¹⁰ , 1×10 ¹¹ , 1×10 ¹² , or more cfu of one or more additional microorganisms (e.g., about 1×10 ⁴ to about 1×10 ⁹ cfu of Bacillus amyloliquefaciens TJ1000 (isolate ATCC BAA-390, also known as 1BE), BRADY, Metarhizium anisopliae F52, Trichoderma virens G1-3, and/or Yersinia entomophaga O82KB8). According to certain embodiments, the compositions of the present disclosure contain at least about 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ , 1×10 ⁸ , per gram and/or milliliter of composition. 1×10 ⁹ , 1×10 ¹⁰ , 1×10 ¹¹ , 1×10 ¹² cfu of one or more additional microorganisms.

In some embodiments, spores from one or more additional microorganisms comprise about 0.1 to about 90% (by weight) of the composition. According to some embodiments, the composition has about , 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 , 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more (by weight) microbial spores from additional microorganisms such as about 10% Bacillus amyloliquefaciens TJ1000, Metarhizium anisopliae F52, Penicillium bilaiae ATCC 20851, Penicillium bilaiae) RS7B-SD1 and/or spores of Trichoderma virens G1-3]. In some embodiments, the amount/concentration of microbial spores from one or more additional microorganisms is about 1 to about 25%, about 5 to about 20%, about 5 to about 15%, about 5 to about 10% or about 8 to about 12% (by weight).

It should be understood that additional microorganisms in the compositions of the present disclosure may include vegetative cells and/or dormant spores. According to certain embodiments, at least 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98, 99% or more additional microorganisms are present in the compositions of the present disclosure as vegetative cells. According to certain embodiments, at least 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98, 99% or more additional microorganisms are present in the compositions of the present disclosure as spores.

Compositions of the present disclosure may be formulated as any suitable type of composition, including, but not limited to, foliar inoculants, seed dressings and soil inoculants.

In some embodiments, compositions of the present disclosure are formulated as amorphous solids.
In some embodiments, compositions of the present disclosure are formulated as amorphous liquids.
In some embodiments, compositions of the present disclosure are formulated as wettable powders.

In some embodiments, the compositions of this disclosure are formulated as liquid compositions that are then dried to produce powders or granules. For example, in certain embodiments, liquid compositions of the present disclosure are drum dried, evaporative dried, fluidized bed dried, freeze dried, spray dried, spray-freeze dried, tray dried and/or vacuum dried to produce powders/granules. dried. Such powders/granules are processed using any suitable method including, but not limited to, flocculation, granulation, and milling to obtain the desired particle size or physical form. can be done. The exact method and parameters of processing the dry powder/granules that are appropriate for a given situation will depend on the desired particle size, type, size and volume of the raw material to which the composition will be applied, the number of microorganisms in the composition. It can be influenced by factors such as species, number of microorganisms in the composition, stability of the microorganisms in the composition, and storage conditions (eg, temperature, relative humidity, duration). Those skilled in the art will understand how to select appropriate methods and parameters using routine experimentation.

In some embodiments, the compositions of this disclosure are frozen for cryopreservation (cryopreservation). For example, in certain embodiments, liquid compositions of the present disclosure are flash frozen and stored in cryopreservation facilities/facilities. The exact method of freezing and preserving the compositions of the present disclosure that is appropriate for a given situation will depend on the type of microorganisms in the composition, the number of microorganisms in the composition, the stability of the microorganisms in the composition and the storage conditions. (e.g. temperature, relative humidity, duration) and other factors. Those skilled in the art will know how to select appropriate methods and parameters using routine experimentation.

Compositions of the present disclosure may be formulated as aqueous or non-aqueous compositions. In some embodiments, the compositions of the present disclosure contain no water. In some embodiments, compositions of the present disclosure contain trace amounts of water. In some embodiments, the composition of the present disclosure contains, by weight based on the total weight of the composition, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, Contains less than 4, 4.25, 4.5, 4.75 or 5% water.

In some embodiments, compositions of the present disclosure are formulated to have a pH of about 4.5 to about 9.5. In some embodiments, compositions of the present disclosure have a pH of about 6 to about 7.5. In some embodiments, the compositions of this disclosure have a pH of about 5, 5.5, 6, 6.5, 7, 7.5, 8 or 8.5.

As noted above, the compositions of the present disclosure can include various carriers, stabilizers, nutrients, pesticides (pesticides), plant signal molecules, dispersants, and the like. The ingredients to be included in the composition and the order in which each ingredient is incorporated into the composition maintains the dispersibility, stability and/or viability of the strains of the present disclosure during storage, distribution and/or application of the composition. can be selected or designed to do or improve.

It should be understood that the compositions of the present disclosure are non-natural (non-naturally occurring) compositions. According to some embodiments, the composition comprises one or more non-natural ingredients. According to some embodiments, the composition comprises non-natural combinations of natural ingredients.

The present disclosure extends to kits comprising, consisting essentially of, or consisting of two or more containers, each of which contain one or more components of the compositions of the present disclosure. For example, one or more strains of the disclosure and an agriculturally acceptable carrier are placed in separate containers for long-term storage and then the composition is combined prior to application to plants or plant propagation material. be able to. Optional components, such as stabilizing compounds, pesticides and plant signaling molecules, are added to either one of the two containers or in one or more separate containers for long-term storage. can accommodate. In some embodiments, the kit further comprises one or more oxygen scavengers such as activated carbon, ascorbic acid, iron powder, a mixture of ferrous carbonate and a metal halide catalyst, sodium chloride and/or sodium bicarbonate. .

The container may comprise any suitable material including, but not limited to, materials that reduce the amount of light, moisture and/or oxygen coming into contact with the coated plant propagation material once the container is sealed. obtain. In some embodiments, the container comprises about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, It comprises, consists essentially of, or consists of a material having a light transmission of 65, 70 or 75%. In some embodiments, the container is about 5,10,15,20,25,30,35,40,45,50,60,70,80,90,100,125,150,175,200,225 , 250, 275, 300, 325, 350, 375, 400, 425, 450, 475 or 500 ^cm3 / ^m2 ·day (as measured in accordance with ASTM D3985) consists of or consists of

In some embodiments, the container reduces the amount of ambient light reaching the coated plant propagation material when sealed to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% reduction.

In some embodiments, the container limits the amount of ambient moisture reaching the coated plant propagation material when sealed to about 5, 10, 15, 20, 25, 30, 35, 40, 45 , 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100%.

In some embodiments, the container reduces the amount of ambient oxygen reaching the coated plant propagation material when sealed to about 5, 10, 15, 20, 25, 30, 35, 40, 45 , 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100%.

The strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure can be applied to any plant species including, but not limited to, row crops and plants. of the family Amaranthaceae (e.g. chard, spinach, sugar beet, or quinoa); of the family Asteraceae (e.g. artichoke, aster, chamomile, chicory, chrysanthemum, dahlia, daisy, echinacea, goldenrod, guayule gum, lettuce , marigolds, safflowers, sunflowers, zinnias); Brassicaceae (e.g. arugula, broccoli, bok choy, Brussels sprouts, cabbage, cauliflower, canola, collard greens, radishes, garden cress, horseradish, kale, mustard greens) , radish, rapeseed, rutabaga, turnip, wasabi, watercress, or Arabidopsis thaliana); Cucurbitaceae (e.g. cantaloupe, cucumber, honeydew melon, melon, pumpkin, squash (e.g. acorn, Japanese squash, summer squash), watermelon, zucchini); Fabaceae (e.g. alfalfa, kidney bean, carob, clover, guar, lentil, mesquite, pea, peanut, soybean, tamarind, tragacanth, broad bean); mallow of the Malvaceae family (e.g. cocoa, cotton, durian, hibiscus, kenaf, kola, okra); , bluegrass, buffalograss, centipede, Fescue or Zoysia), millet, oats, ornamental grasses, rice, rye, sorghum, sugarcane, triticale, or wheat] of Polygonaceae (e.g. buckwheat); of Rosaceae (e.g. almonds, apples, apricots, blackberries, blueberries, cherries, peaches, plums, quince, raspberries, roses, strawberries); Solanaceae of the Solanaceae (e.g. peppers, chili peppers, eggplants, petunias, potatoes, tobacco, tomatoes); In some embodiments, strains, cultures, Cell-free filtrates, cell extracts, and inoculum compositions may contain one or more plants not naturally associated with the strain (e.g., one or more plants not naturally occurring in the geographic location from which the strain was isolated). plants) can be formulated for treatment. In some embodiments, the strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure contain one or more acaricide-, fungicide-, gastropode-, herbicide-, Insecticide-, nematicide-, rodenticide- and/or virucidal-tolerant plants [e.g. acetolactate synthase inhibitors (e.g. imidazolinones, pyrimidinyloxy(thio)benzoates, sulfonylaminocarbonyltriazolinones, sulfonylureas, triazolopyrimidines), bialaphos, glufosinate, glyphosate, hydroxyphenylpyruvate dioxygenase inhibitors and/or phosphinothricins]. Non-limiting examples of strains, cultures, cell-free filtrates, cell extracts, and inoculum compositions of the present disclosure include BOLLGARD II®, DROUGHTGARD®, GENUITY®, RIB COMPLETE® Trademarks), ROUNDUP READY®, ROUNDUP READY 2 YIELD®, ROUNDUP READY 2 EXTEND ^™ ®, SMARTSTAX®, VT DOUBLE PRO®, VT TRIPLE PRO®, Plants sold under the trade names YIELDGARD®, YIELDGARD VT ROOTWORM/RR2®, YIELDGARD VT TRIPLE® and/or XTENDFLEX ^™™ .

Strains, cultures, cell-free filtrates, cell extracts, and inoculum compositions of the present disclosure are available in ACCELERON®, ACTINOVATE®, CELL-TECH®, JUMPSTART®, MET52 ( ®, NEMASTRIKE ^™ , NITRAGIN ® , OPTIMIZE ® , QUICKROOTS ® , TAGTEAM ® and/or TORQUE ® products. can. Strains of the present disclosure include AVAIL®, BAR MAX NORTE, BAR MAX SUR, BIOBOOST®, BIOPOWER, BIOSINC®, COMO PLATINUM, CROP+®, DEFENDR™, DIAMONBRAND ( DYNA-START(TM), EXCALIBRE-SA(TM), EXCEED(TM), EXCELLORATE(TM), FIRST UP(TM), FLEXCONNECT(TM), FORZA(TM), FUNGI-PHITE(TM) (registered trademark), GRAPH-EX (registered trademark), GRAPH-EX SA (registered trademark), GUARD N (registered trademark), HEADSUP (registered trademark), ILEVO (registered trademark), INTRACEPT (registered trademark), LAUNCHER (registered trademark) , LEGACY(TM), MARAUDER(R), MASTERFIX L PREMIER, MAXIMIZE(TM), MEGAPACK(TM), MICROAZ-IF LIQUID(TM), MICROAZ-ST DRY(TM), MICROSTAR(R), MICROSYNC(TM) Trademark), MORE THAN MANURE®, NATURAL®, N-CHARGE®, N-DURE®, N-FORCE, N-TAKE®, NODULATOR®, NUE CHARGE G™, NUTRI-GROW™, NUTRIPACTION™, NUTRI-PHITE™, NUTRISPHERE-N™, OBVIUS™, PBX, PONCHO™, PREMAX (registered trademark), PREMAXR (registered trademark), PRE-VAIL (registered trademark), PRESIDE CL (registered trademark), PRESIDE ULTRA (registered trademark), PRIMACY ALPHA (registered trademark), PRIMO, PROSURGE (registered trademark), PULSERHIZO (registered trademark) ), RECOVER®, SABREX®, RILEGUM®, RIZOFOS®, RIZOLIQ®, SAFE ZONE®, SEED+®, SIGNUM®, SIMBIOSE (registered trademark), SOYRHIZO (registered trademark), SOYSUPERB ( STAMINA®, STATUS®, STERICS®, STIMUCONTROL®, SYSTIVA®, TAKE OFF®, TAKE OFF ST®, TERRAMAX DRY(TM), TERRAMAX LIQUID-IF, TRIDENT(TM), TUXEDO(R), VAULT(R), VERTEX-IF, VIGOR(R), VIGOR SEED, VOTIVO(R), WUXAL TERIOS and XITEBIO ® YIELD+ can be beneficially incorporated into products.

The strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure can be applied to any site/part of the plant. In some embodiments, one or more strains of the disclosure (or inoculum compositions of the disclosure) are applied to plant propagation material (eg, cuttings, rhizomes, seeds and tubers). In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the disclosure are applied to plant roots. In some embodiments, strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the disclosure are applied to the foliage of plants. In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the disclosure are applied to both roots and foliage of plants. In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure are applied to plant propagation material and to plants grown from said plant propagation material. be.

The strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure can be applied to any plant growth medium, including but not limited to soil.

The strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure may be applied by any suitable method, including, but not limited to, seed application, furrow application and foliar application, to plants, It can be applied to the plant part and/or the plant growth medium.

Strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure may be prepared in any suitable manner including, but not limited to, coating, dripping, dressing, encapsulating, submerging, spraying and immersing. , can be applied. A batch mode can be used in which a predetermined batch size of feedstock and inoculum composition is delivered to the mixer. A continuous process mode can also be used in which the inoculum composition is adjusted to drop the inoculum composition at a defined rate into the continuous stream of feedstock.

In some embodiments, one or more strains, cultures, cell-free filtrates and/or inoculum compositions of the disclosure are applied directly to plant propagation material (eg seeds). According to certain embodiments, the plant propagation material is at least 0.1, 0.2, 0.3, 0.4, 0.5 , 0.6, 0.7, 0.8, 0.9, 1, 1.25, 1.5, 1.75, 2, 3, 4, 5, 6, 9, 12, 15, 18, 21, 24, 36, 48 hours. According to certain embodiments, plant propagation material is coated with one or more strains, cultures, cell-free filtrates and/or inoculum compositions of the present disclosure. Plant propagation material may be included in one or more additional layers (e.g., one or more protective layers that serve to improve the stability and/or viability of strains of the present disclosure and/or within the same layer). It may also be coated with one or more isolation layers) containing substances that may reduce the stability and/or viability of the strains of the present disclosure. In some embodiments, the coating comprises, consists essentially of, or consists of an inoculum composition of the present disclosure and a dry powder.

In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the disclosure are applied directly to plant growth media (eg, soil). In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the disclosure are applied near plant propagation material (eg, seeds).
In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure are applied to the root zone of plants. According to certain embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the disclosure are applied using a drip irrigation system.

In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the disclosure are applied directly to plants. According to certain embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the disclosure are sprayed and/or watered onto the plants to be treated.

In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure are freeze-spray- or spray-freeze-dried and then plant/plant Applied to the site. For example, in some embodiments, an inoculum composition comprising one or more strains of the present disclosure and one or more stabilizing components (eg, one or more maltodextrins having a DEV of about 15 to about 20) is , freeze-spray- or spray-freeze-dried, dry powders [e.g. calcium stearate, attapulgite clay, montmorillonite clay, graphite, magnesium stearate, silica (e.g. fumed silica, hydrophobically coated silica and/or precipitated silica). ) and/or talc] and then an inoculant formulation comprising one or more adhesives (e.g., one or more maltodextrins, one or more monosaccharides, di- or oligosaccharides, one or more peptones, etc.). substances), one or more pesticides and/or one or more plant signal molecules (eg one or more LCOs).

The strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure can be applied to plants, plant parts and/or plant growth media in any suitable amount and/or concentration.

In some embodiments, one or more strains of the present disclosure are applied at a rate of about 1×10 ¹ to about 1×10 ²⁰ cfu per kilogram of plant propagation material. According to some embodiments, one or more strains of the present disclosure are used in plant propagation material at about/at least 1 x 104, ¹ x ¹⁰⁵ , 1 x ¹⁰⁶ , 1 per kilogram of said plant propagation material. reliably coated with ^x107 , ¹ x ¹⁰⁸ , ¹ x 109, ¹ x ¹⁰¹⁰ , ¹ x 1011, ¹ x 1012, 1 x 1013, 1 x 1014, ¹ x 1015 cfu applied in an amount sufficient to According to some embodiments, one or more strains of the present disclosure average about/at least 1 x 103, 1 x ¹⁰⁴ , 1 x ¹⁰⁵ , 1 x ¹⁰⁶ , 1 x ^{107 ,} 1 x to ensure that 10 ⁸ , 1 x 10 ⁹ , 1 x 10 ¹⁰ , 1 x 10 ¹¹ , 1 x 10 ¹² , 1 x 10 ¹³ , 1 x 10 ¹⁴ , 1 x 10 ¹⁵ cfu are applied to each seed. Applied in sufficient quantity.

In some embodiments, one or more strains of the present disclosure are applied at a rate of about 1 x 101 to about ¹ x ¹⁰²⁰ cfu per plant. According to some embodiments, one or more strains of the present disclosure ensure that each plant is about/at least 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ , 1×10 applied in an amount sufficient to be treated with ⁸ , ¹ x ¹⁰⁹ , ¹ x ¹⁰¹⁰ , ¹ x 1011, ¹ x 1012, 1 x 1013, 1 x 1014, ¹ x 1015 cfu . According to some embodiments, one or more strains of the present disclosure average about/at least 1 x 103, 1 x ¹⁰⁴ , 1 x ¹⁰⁵ , 1 x ¹⁰⁶ , 1 x ^{107 ,} 1 x enough to ensure that 10 ⁸ , 1 x 10 ⁹ , 1 x 10 ¹⁰ , 1 x 10 ¹¹ , 1 x 10 ¹² , 1 x 10 ¹³ , 1 x 10 ¹⁴ , 1 x 10 ¹⁵ cfu are applied to each plant. applied in large amounts.

In some embodiments, one or more strains of the present disclosure are applied at a rate of from about 1 x 101 to about ¹ x 1020 cfu per acre ⁽ about 4047 ^m2 ) of treated crop. According to some embodiments, one or more strains of the present disclosure ensure that each acre of treated crop is about/at least 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ , 1×10 ⁸ , 1×10 ⁹ , 1×10 ¹⁰ , 1×10 ¹¹ , 1×10 ¹² , 1×10 ¹³ , 1×10 ¹⁴ , 1×10 ¹⁵ cfu applied in According to some embodiments, one or more strains of the present disclosure average about/at least 1 x 103, 1 x ¹⁰⁴ , 1 x ¹⁰⁵ , 1 x ¹⁰⁶ , 1 x ^{107 ,} 1 x Ensure that ¹⁰⁸ , ¹ x 109, ¹ x ¹⁰¹⁰ , ¹ x 1011, ¹ x 1012, 1 x 1013, 1 x ¹⁰¹⁴ , ¹ x 1015 cfu are applied to each acre of treated crops. applied in sufficient quantity to

In some embodiments, one or more strains of the present disclosure are applied at a rate of about 1×10 ¹ to about 1×10 ²⁰ cfu per acre of plant growth medium. According to some embodiments, one or more strains of the present disclosure ensure that each acre of plant growth medium is about/at least 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 enough to be treated with ⁷ , 1 x ¹⁰⁸ , ¹ x ¹⁰⁹ , ¹ x ¹⁰¹⁰ , ¹ x 1011, ¹ x 1012, 1 x 1013, 1 x 1014, ¹ x 1015 cfu applied in quantity. According to some embodiments, one or more strains of the present disclosure average about/at least 1 x 103, 1 x ¹⁰⁴ , 1 x ¹⁰⁵ , 1 x ¹⁰⁶ , 1 x ^{107 ,} 1 x Ensure ¹⁰⁸ , ¹ x 109, ¹ x ¹⁰¹⁰ , ¹ x 1011, ¹ x 1012, 1 x 1013, 1 x ¹⁰¹⁴ , ¹ x 1015 cfu applied to each acre of plant growth medium applied in an amount sufficient to

In some embodiments, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure are applied at a rate of about 0.05 to about 100 milliliters and/or grams per kilogram of plant propagation material. According to some embodiments, one or more of the cultures, cell-free filtrates, cell extracts or inoculum compositions of the present disclosure contain about/at least 0.05, 0.1, 0.125, 0.15, 0.175 per kilogram of plant propagation material. , 0.2, 0.225, 0.25, 0.275, 0.3, 0.325, 0.35, 0.375, 0.4, 0.425, 0.45, 0.475, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5 , 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 20 , 30, 40, 50, 60, 70, 80, 90 or 100 milliliters and/or grams is applied in an amount sufficient to ensure coating. According to some embodiments, one or more of the cultures, cell-free filtrates, cell extracts or inoculum compositions of the present disclosure average about/at least 0.05, 0.1, 0.125, 0.15, 0.175, 0.2, 0.225, 0.25, 0.275, 0.3, 0.325, 0.35, 0.375, 0.4, 0.425, 0.45, 0.475, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, Sufficient amounts are used to ensure that 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75 or 5 milliliters/gram are applied to each seed.

In some embodiments, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure are applied at a rate of about 0.5 to about 100 milliliters and/or grams per plant. According to some embodiments, one or more of the cultures, cell-free filtrates, cell extracts or inoculum compositions of the present disclosure ensure that each plant contains about/at least 0.05, 0.1, 0.125, 0.15, 0.175, 0.2, 0.225, 0.25, 0.275, 0.3, 0.325, 0.35, 0.375, 0.4, 0.425, 0.45, 0.475, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 20, It is applied in an amount sufficient to treat 30, 40, 50, 60, 70, 80, 90 or 100 milliliters and/or grams. According to some embodiments, one or more of the cultures, cell-free filtrates, cell extracts or inoculum compositions of the present disclosure average about/at least 0.05, 0.1, 0.125, 0.15, 0.175, 0.2, 0.225, 0.25, 0.275, 0.3, 0.325, 0.35, 0.375, 0.4, 0.425, 0.45, 0.475, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, Sufficient amounts are used to ensure that 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75 or 5 milliliters/gram are applied to each plant.

In some embodiments, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure are applied at a rate of about 0.05 to about 100 milliliters and/or grams per acre of treated crop. According to some embodiments, one or more of the cultures, cell-free filtrates, cell extracts or inoculum compositions of the present disclosure provide about/at least 0.05, 0.1, 0.125, 0.15, 0.175, 0.2, 0.225, 0.25, 0.275, 0.3, 0.325, 0.35, 0.375, 0.4, 0.425, 0.45, 0.475, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, Quantities of 20, 30, 40, 50, 60, 70, 80, 90 or 100 milliliters and/or grams are used in amounts sufficient to ensure coating. According to some embodiments, one or more of the cultures, cell-free filtrates, cell extracts or inoculum compositions of the present disclosure average about/at least 0.05, 0.1, 0.125, 0.15, 0.175, 0.2, 0.225, 0.25, 0.275, 0.3, 0.325, 0.35, 0.375, 0.4, 0.425, 0.45, 0.475, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75 or 5 milliliters/gram are applied to each acre of treated crop.

In some embodiments, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure are applied at a rate of about 0.05 to about 100 milliliters and/or grams per acre of plant growth medium. According to some embodiments, one or more of the cultures, cell-free filtrates, cell extracts or inoculum compositions of the present disclosure are used in each acre of plant growth medium at about/at least 0.05, 0.1, 0.125, 0.15 , 0.175, 0.2, 0.225, 0.25, 0.275, 0.3, 0.325, 0.35, 0.375, 0.4, 0.425, 0.45, 0.475, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25 , 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10 , 20, 30, 40, 50, 60, 70, 80, 90 or 100 milliliters and/or grams are used in amounts sufficient to ensure coating. According to some embodiments, one or more of the cultures, cell-free filtrates, cell extracts or inoculum compositions of the present disclosure average about/at least 0.05, 0.1, 0.125, 0.15, 0.175, 0.2, 0.225, 0.25, 0.275, 0.3, 0.325, 0.35, 0.375, 0.4, 0.425, 0.45, 0.475, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75 or 5 milliliters/gram are applied to each acre of plant growth medium.

In some embodiments, the culture/inoculum composition of the present disclosure contains about/at least 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ , 1× per kilogram of plant propagation material. ¹⁰⁸ , ¹ x 109, ¹ x ¹⁰¹⁰ , ¹ x 1011, ¹ x 1012, ¹ x 1013, 1 x 1014, ¹ x 1015 cfu of Erwinia (e.g. E. ) is used in an amount sufficient to ensure coating. According to some embodiments, one or more culture/inoculum compositions of the present disclosure average about/at least about 1 x ¹⁰³ , 1 x ¹⁰⁴ , 1 x ¹⁰⁵ , 1 x ¹⁰⁶ , 1 ×10 ⁷ , 1 × 10 ⁸ , 1 × 10 ⁹ , 1 × 10 ¹⁰ , 1 × 10 ¹¹ , 1 × 10 ¹² , 1 × 10 ¹³ , 1 × 10 ¹⁴ , 1 × 10 ¹⁵ cfu of Erwinia (e.g. E billigiae NRRL B-67766) is used in sufficient quantity to ensure application to each seed.

In some embodiments, the culture/inoculum composition of the present disclosure contains about/at least 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ , 1×10 ⁸ , 1×10 8 , 1×10 5 each plant. ^x109 , ¹ x ¹⁰¹⁰ , ¹ x 1011, ¹ x 1012, ¹ x 1013, 1 x 1014, ¹ x 1015 cfu Erwinia (e.g. E. billigiae NRRL B-67766) reliably Used in amounts sufficient to be processed. According to some embodiments, one or more culture/inoculum compositions of the present disclosure average about/at least about 1 x ¹⁰³ , 1 x ¹⁰⁴ , 1 x ¹⁰⁵ , 1 x ¹⁰⁶ , 1 ×10 ⁷ , 1 × 10 ⁸ , 1 × 10 ⁹ , 1 × 10 ¹⁰ , 1 × 10 ¹¹ , 1 × 10 ¹² , 1 × 10 ¹³ , 1 × 10 ¹⁴ , 1 × 10 ¹⁵ cfu Erwinia (e.g. E. billigiae NRRL B-67766) is used in an amount sufficient to ensure application to each plant.

In some embodiments, the culture/inoculum composition of the present disclosure is about/at least 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ , 1×10 ⁸ per acre of treated crop. , 1×10 ⁹ , 1×10 ¹⁰ , 1×10 ¹¹ , 1×10 ¹² , 1×10 ¹³ , 1×10 ¹⁴ , 1×10 ¹⁵ cfu of Erwinia (e.g. E. billigiae NRRL B-67766) Used in sufficient quantity to ensure processing. According to certain embodiments, one or more cultures/compositions of the present disclosure average about/at least 1×10 ³ , 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ , 1×10 ⁸ , 1×10 ⁹ , 1×10 ¹⁰ , 1×10 ¹¹ , 1×10 ¹² , 1×10 ¹³ , 1×10 ¹⁴ , 1×10 ¹⁵ cfu of Erwinia (e.g. E. billigiae NRRL B -67766) is used in amounts sufficient to ensure coverage of each acre of treated crop.

In some embodiments, the culture/inoculum composition of the present disclosure is about/at least 1 x ¹⁰⁴ , 1 x ¹⁰⁵ , 1 x ¹⁰⁶ , 1 x ¹⁰⁷ , 1 x ¹⁰⁸ , 1 x ¹⁰⁹ , 1×10 ¹⁰ , 1×10 ¹¹ , 1×10 ¹² , 1×10 ¹³ , 1×10 ¹⁴ , 1×10 ¹⁵ cfu of Erwinia (e.g. E. billigiae NRRL B-67766). used in sufficient quantity to According to certain embodiments, one or more cultures/compositions of the present disclosure average about/at least 1×10 ³ , 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ , 1×10 ⁸ , 1×10 ⁹ , 1×10 ¹⁰ , 1×10 ¹¹ , 1×10 ¹² , 1×10 ¹³ , 1×10 ¹⁴ , 1×10 ¹⁵ cfu Erwinia (e.g. E. billigiae NRRL B- 67766) is used in an amount sufficient to ensure that each acre of plant growth medium is applied.

In some embodiments, the culture/inoculum composition of the present disclosure contains about/at least 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ , 1× per kilogram of plant propagation material. 10 ⁸ , 1 x 10 ⁹ , 1 x 10 ¹⁰ , 1 x 10 ¹¹ , 1 x 10 ¹² , 1 x 10 ¹³ , 1 x 10 ¹⁴ , 1 x 10 ¹⁵ cfu of Pseudomonas spp. ) NRRL B-67767 and/or P. libanesis NRRL B-67769). According to some embodiments, one or more culture/inoculum compositions of the present disclosure average about/at least about 1 x ¹⁰³ , 1 x ¹⁰⁴ , 1 x ¹⁰⁵ , 1 x ¹⁰⁶ , 1 ×10 ⁷ , 1 × 10 ⁸ , 1 × 10 ⁹ , 1 × 10 ¹⁰ , 1 × 10 ¹¹ , 1 × 10 ¹² , 1 × 10 ¹³ , 1 × 10 ¹⁴ , 1 × 10 ¹⁵ cfu of Pseudomonas spp. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769) is used in an amount sufficient to ensure application to each seed.

In some embodiments, the culture/inoculum composition of the present disclosure contains about/at least 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ , 1×10 ⁸ , 1×10 8 each plant. ×10 ⁹ , 1 × 10 ¹⁰ , 1 × 10 ¹¹ , 1 × 10 ¹² , 1 × 10 ¹³ , 1 × 10 ¹⁴ , 1 × 10 ¹⁵ cfu of Pseudomonas (e.g. P. gesseardii) NRRL B- 67767 and/or P. libanesis NRRL B-67769). According to some embodiments, one or more culture/inoculum compositions of the present disclosure average about/at least about 1 x ¹⁰³ , 1 x ¹⁰⁴ , 1 x ¹⁰⁵ , 1 x ¹⁰⁶ , 1 ×10 ⁷ , 1 × 10 ⁸ , 1 × 10 ⁹ , 1 × 10 ¹⁰ , 1 × 10 ¹¹ , 1 × 10 ¹² , 1 × 10 ¹³ , 1 × 10 ¹⁴ , 1 × 10 ¹⁵ cfu of Pseudomonas spp. Sufficient amounts of P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769) are used to ensure that each plant is treated.

In some embodiments, the culture/inoculum composition of the present disclosure provides about/at least 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ , 1× 10 ⁸ , 1 x 10 ⁹ , 1 x 10 ¹⁰ , 1 x 10 ¹¹ , 1 x 10 ¹² , 1 x 10 ¹³ , 1 x 10 ¹⁴ , 1 x 10 ¹⁵ cfu of Pseudomonas spp. ) NRRL B-67767 and/or P. libanesis NRRL B-67769). According to some embodiments, one or more culture/inoculum compositions of the present disclosure average about/at least about 1 x ¹⁰³ , 1 x ¹⁰⁴ , 1 x ¹⁰⁵ , 1 x ¹⁰⁶ , 1 ×10 ⁷ , 1 × 10 ⁸ , 1 × 10 ⁹ , 1 × 10 ¹⁰ , 1 × 10 ¹¹ , 1 × 10 ¹² , 1 × 10 ¹³ , 1 × 10 ¹⁴ , 1 × 10 ¹⁵ cfu of Pseudomonas spp. P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769) is used in an amount sufficient to ensure that each acre of treated crop is applied.

In some embodiments, the culture/inoculum composition of the present disclosure provides about/at least 1 x 10 ⁴ , 1 x 10 ⁵ , 1 x 10 ⁶ , 1 x 10 ⁷ , 1 x 10 7 , 1 x 10 7 , 1 x 10 5 , 1 x 10 7 , 1 x 10 5 , 1 x 10 5 , 1 x 10 5 , 1 x 10 5 , 1 x 10 5 acres of plant growth medium each. ×10 ⁸ , 1 × 10 ⁹ , 1 × 10 ¹⁰ , 1 × 10 ¹¹ , 1 × 10 ¹² , 1 × 10 ¹³ , 1 × 10 ¹⁴ , 1 × 10 ¹⁵ cfu of Pseudomonas (e.g. P. gesardi (P. gesseardii) NRRL B-67767 and/or P. libanesis NRRL B-67769). According to some embodiments, one or more culture/inoculum compositions of the present disclosure average about/at least about 1 x ¹⁰³ , 1 x ¹⁰⁴ , 1 x ¹⁰⁵ , 1 x ¹⁰⁶ , 1 ×10 ⁷ , 1 × 10 ⁸ , 1 × 10 ⁹ , 1 × 10 ¹⁰ , 1 × 10 ¹¹ , 1 × 10 ¹² , 1 × 10 ¹³ , 1 × 10 ¹⁴ , 1 × 10 ¹⁵ cfu of Pseudomonas spp. P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769) is used in an amount sufficient to ensure that each acre of plant growth medium is applied.

Strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure may be used for, but not limited to, germination, post-emergence, pre-seedling, post-seedling, pre-vegetative, mid-vegetative, vegetative plants at any time, including post-, pre-reproductive, mid-reproductive, post-reproductive, pre-flowering, at flowering, post-flowering, pre-fruiting, during fruiting, post-fruiting, pre-ripening, ripening and post-ripening In addition, it can be applied to the plant part and/or to the plant growth medium.

In certain embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure are inoculated about/at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, Applied 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks prior.

In certain embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the disclosure are applied to plant propagation material (eg, seeds) at the time of planting.

In certain embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the disclosure are applied to plant propagation material (e.g., seeds) after planting and prior to germination. .

In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the disclosure are applied to plants post-emergence.

In certain embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure are applied to plants or plant parts post-harvest (e.g., after a post-harvest storage period). to control pests during the period).

The present disclosure provides for the use of one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure in plants (e.g. coated plant propagation material) treated with one or more strains, cultures, cell extracts and/or inoculum compositions of the present disclosure. one or more strains, to a plant part (e.g. coated plant propagation material) treated with an inoculum composition treated with the above strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions; one or more strains, cultures, cell-free filtrates on plant parts harvested from plants (e.g., coated plant propagation material) treated with cultures, cell-free filtrates, cell extracts and/or inoculum compositions , a plant part harvested from a plant growing from a plant part (e.g., a coated plant propagation material) treated with a cell extract and/or an inoculum composition, one or more strains of the present disclosure, cultures, One or more strains, cultures of the present disclosure are added to processed products obtained from plants grown from plant parts (e.g., coated plant propagation material) treated with cell filtrates, cell extracts and/or inoculum compositions. , the cell-free filtrate, cell extract and/or inoculum composition of the present disclosure to a crop comprising a plurality of plants treated with the cell-free filtrate, cell extract and/or inoculum composition (e.g. coated plant propagation material). extends to crops comprising multiple plants growing from plant parts treated with substances (eg, coated plant propagation material).

In some embodiments, the present disclosure comprises, consists essentially of, or consists of a plant propagation material and a coating covering at least a portion of an exterior surface of the plant propagation material, wherein the coating is one of the present disclosure. It comprises, consists essentially of, or consists of more than one species of strain, cell-free filtrate, cell extract and/or inoculum composition.

In some embodiments, the coating can include 1, 2, 3, 4, 5 or more layers. In some embodiments, the coating comprises an inner layer containing one or more strains, cultures, cell-free filtrates and/or cell extracts of the present disclosure and strains, cultures, cell-free filtrates and/or cell extracts. free or essentially free of one or more outer layers. In some embodiments, the coating is equivalent to the inoculation composition of the present disclosure except that it does not include an inner layer that is an inoculum composition of the present disclosure and said strain, culture, acellular filtrate and/or cell extract. and an outer layer.

In some embodiments, the coating comprises, consists essentially of, or consists of a culture/inoculum composition of the present disclosure and a dry powder. Dry powders can be applied in any suitable amount/concentration. The absolute value of the amount/concentration sufficient to elicit the desired effect depends on the type, size and volume of material to which the composition is to be applied, the type of microorganisms in the composition, the number of microorganisms in the composition. May be affected by factors such as stability, as well as storage conditions (eg, temperature, relative humidity, duration). Those of ordinary skill in the art will understand how to select effective amounts/concentrations using routine dose-response studies. The selection of suitable amounts/concentrations can be found, for example, in International Patent Publication Nos. WO2017/210166 and U.S. Provisional Patent Application Nos. 62/296,798; 62/271,857; 62/347,773; 62/343,217; 62/296,784; 62/347,785; 62/347,794; and 62/347,805. In embodiments, the dry powder can be applied in any suitable amount/concentration. In some embodiments, the dry powder is applied in an amount ranging from about 0.5 to about 10 grams of dry powder per kilogram of plant propagation material. For example, in certain embodiments, about 0.5, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75, 5, 5.5, per kilogram of seed, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10 grams (g) or more of dry powder (e.g. dry powder containing magnesium stearate, magnesium sulfate, milk powder, silica, soy lecithin and/or talc) applied. In some embodiments, dry powders comprising calcium stearate, attapulgite clay, montmorillonite clay, graphite, magnesium stearate, silica (fumed silica, hydrophobically coated silica, and/or precipitated silica) and/or talc are added to the seeds. An inoculum composition of the present disclosure is applied to the coated seeds at a rate of about 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75 or 3 grams per kilogram.

In some embodiments, the coating completely covers the exterior surface of the plant propagation material.

In some embodiments, the average thickness of the coating is at least 3.3, 3.4, 3.5, 4, 4.5, 5 μm or more. In some embodiments, the coating has an average thickness of about 1.5 to about 3.0 μm.

The present disclosure provides one or more plants and/or plant parts that have been treated with one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure; a kit comprising, consisting essentially of, or consisting of a container for housing plants and/or plant parts. In some embodiments, the kit further includes one or more oxygen scavengers, such as activated carbon, ascorbic acid, iron powder, a mixture of ferrous carbonate and a metal halide catalyst, sodium chloride, and/or sodium bicarbonate. Including further.

The container can comprise any suitable material including, but not limited to, materials that reduce the amount of light, moisture and/or oxygen coming into contact with the coated plant propagation material once the container is closed. In some embodiments, the container is about , comprising, consisting essentially of, or consisting of a material having a light transmission of less than 70 or 75%. In some embodiments, the container is about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 125 (measured according to ASTM D3985). , 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475 or 500 ^cm3 / ^m2.day consisting essentially of or consisting of

In certain embodiments, the container, when closed, reduces the amount of ambient light reaching said coated plant propagation material by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 , 60, 65, 70, 75, 80, 85, 90, 95 or 100%.

In certain embodiments, the container, when closed, reduces the amount of environmental humidity (moisture) reaching said coated plant propagation material by about 5, 10, 15, 20, 25, 30, 35, 40, 45, Reduce by 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100%.

In certain embodiments, the container, when closed, reduces the amount of ambient oxygen reaching said coated plant propagation material by about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55. , 60, 65, 70, 75, 80, 85, 90, 95 or 100%.

In certain embodiments, kits of the present disclosure include 1, 2, 3, 4, 5 or more additional containers. This additional container can contain any suitable ingredient or composition including, but not limited to, agronomically beneficial microorganisms, biostimulants, desiccants, nutrients, oxidation control ingredients and pesticides. Examples of agronomically beneficial microorganisms, biostimulants, desiccants, nutrients, oxidation control ingredients and pesticides that can be included in the additional container are described above.

The present disclosure is an animal feed composition comprising, consisting essentially of, or consisting of a food component and a microbial component, wherein said microbial component is one or more strains, cultures, cell-free filtrates of the present disclosure. Also provided is an animal feed composition comprising, consisting essentially of, or consisting of, a cell extract, and/or an inoculum composition.

Animal feed compositions of the present disclosure can be any animal feed including, but not limited to, feed (e.g., cereals, hay, legumes, silage and/or straw) and forage (e.g., grasses). It may contain suitable food ingredients.

The animal feed compositions of the present disclosure can be fed to any suitable animal including, but not limited to, livestock, zoo animals, laboratory animals and/or companion animals (pets). In some embodiments, the animal feed composition is avian (eg, chicken, duck, quail and/or turkey), bovine (eg, antelope, bison, cow, gazelle, goat, impala, bull, sheep and/or or wild beast), canines, cervids (e.g. caribou, deer, elk and/or moose), equids (e.g. donkeys, horses and/or zebras), felines, fish, pigs, rabbits , rodents (eg, guinea pigs, hamsters, mice and/or rats).

The present disclosure extends to methods and uses of strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure.

In certain embodiments, the methods and uses of the present disclosure involve applying one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure to a plant or plant part (e.g., plant propagation material). including, consisting essentially of, or consisting of applying. The strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure may be applied to any type of plant, to any part/part of the plant, in any suitable manner, in any suitable amount/ It can be applied in concentrations and at any suitable time.

In certain embodiments, a method of the present disclosure comprises applying one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure to a plant growth medium, or then consisting essentially of or consisting of The strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure may be added to any plant growth medium in any suitable manner, in any suitable amount/concentration and in any suitable can be applied at any time.

In certain embodiments, the methods and uses of the present disclosure involve plants or plant parts that have been pretreated with one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure (e.g. plant propagation material) into a plant growth medium (eg, soil). Such methods further comprise, consist essentially of, or consist of introducing one or more nutrients (eg, nitrogen and/or phosphorus) into the plant growth medium. Any suitable nutrient can be added to the growth medium, including but not limited to rock phoshate, monoammonium phosphate, diammonium phosphate, monocalcium phosphate, superphosphate, Included are heavy superphosphates, ammonium polyphosphates, fertilizers containing one or more phosphorus sources, and combinations thereof.

In certain embodiments, the methods of the present disclosure grow plants from plant propagation material that has been pretreated with one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure. comprising, consisting essentially of, or consisting of

Strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure can be made from cereals and pseudocereals such as, but not limited to, barley, buckwheat, corn, millet, oats, quinoa, rice, rye, Growing and growing various plants, including sorghum and wheat, and legumes such as alfalfa, legumes, carob, bush clover, guar, lentils, mesquite, peas, peanuts, soybeans, tamarind, tragacanth and vetch /or can be used to protect and improve yield.

The strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure are suitable for many plant pests, including but not limited to fungi and fungi-like organisms [e.g. Ascomycetes, Basidiomycota. soil fungi from steels of the phylum Basidiomycetes, Chytridiomycetes, Deuteromycetes, Peronosporomycetes (syn., Oomycetes), Plasmodiophoromycetes and Zygomycetes] It can be used to prevent and/or treat infection/spread. In some embodiments, the strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure are strains of Albugo (e.g., A. candida), Alternaria ( Alternaria (e.g. A. alternata, A. solani), Aphanomyces, Armillaria, Ascomycota, Aspergillus (e.g. , A. candidus, A. clavatus, A. flavus, A. fumigatus, A. parasiticus, A. restricticus ( A. restrictus, A. sojae, A. solani, Bipolaris, Blumeria (e.g. B. graminis) , Boeremia, Botrytis (e.g. B. cinerea), Calonectria, Ceratocystis, Cercospora (e.g. C. soina (C. sojina), C. zeae-maydis), Chaetomium, Cladosporum (e.g. C. cladosporioides), Claviceps Claviceps (e.g. C. purpurea), Colletotrichum (Anthracnose) (e.g. C. acutatum, C. boninense, C. capsici ( C. capsici), C. caudatum, C. coccodes, C. crassipes, C. dematium, C. destructivum (C. destructivum), C. fragariae, C. gloeosporioides, C. graminicola, C. kehawae, C. lindemithianum ), C. musae, C. orbiculare, C. spinaceae, C. sublineolum, C. trifolii, C. trancatum (C. truncatum), Coniella, Coniophora, Cryphonectria, Curvularia, Diplocarpon, Exserohilum (e.g., E. E. turcicum, Fibroporia, Fusarium (e.g. F. graminearum, F. moniliforme, F. oxysporum (wilt pathogens) ), F. roseum, F. tricinctum, F. virguliforme), Gaummannomuces, Gloeosporium, Gignardia ( Guignardia), Helminthosporium, Magnaporthe (e.g. M. grisea (Crabgrass blast fungus), M. oryzae (rice blast fungus)), Melampsora Genus Melamspora (e.g. M. lini), Microdochium (e.g. M. nivale), Monilinia, Mycoshaerella (e.g. M. graminicola (M. graminicola), genus Nematospora (N ematospora), Neofabraea, Neofusicoccum, Penicillium (e.g. P. rugulosum, P. verrucosum), Peronosporaceae ), Phakopsora (e.g. P. pachyrhizi (soybean rust)), Phoma, Phomopsis, Physoderma, Phytophthora (late blight) fungi) (e.g. P. infestans), Phytomyxea, Phytoplasma, Pilidium, Plasmodiophora, Plasmospora (e.g. P. infestans) Vitokola), Pleiochaeta, Plenodomus, Poria, Protostegia, Pseudopersonospora (e.g. P. cubensis), Pseudopily Pseudopyricularia, Puccinia (rust fungi) (e.g. P. graminis, P. striiformis, P. tritici, P. Triticina (P. triticina), Pucivinia (e.g. P. graministice), Pyrenophora, Pythium (Pythium), Rhizoctonia (e.g. P. graministice) R. solani (leaf blight, take-off pathogen)), Rhizopus, Saccharata, Sclerotinia (e.g. S. sclerotiorum), Sclerotium Genus (Sclerotium), Scop Scopulariopsis, Septoria (e.g. S. tritici), Selerotinia, Serpula, Setosphaeria (e.g. S. turcicum) ), Spiroplasma, Spongospora, Stenocarpella, Synchytrium, Taphyrina, Thielaviopsis, Thyrostroma, Ustilago (e.g. U. maydis (corn smut)), Venturia (spot), Verticillium (wilt fungus), Wilsonomyces or Zymoceptria ( Zymoseptoria) is effective in killing, inhibiting its growth and/or inhibiting its reproduction/reproduction. Additional examples of fungi and fungi-like organisms that can be treated with the strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure can be found in Bradley, Managing Diseases, in Illinois Agronomy Handbook (2008). be able to.

Non-limiting examples of plant symptoms/diseases for which the strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure can be used to prevent and/or treat anthracnose, apple scab , bacterial canker (canker), black knot, blight (e.g., chestnut blight, early blight, burn blight, leaf blight, potato leaf blight, wheat leaf blight) , flower end rot, end rot, black root rot, black spot, spot, brown spot, brown rot, canker, clubroot, cotton rot, wilt, rot, dollar spot, Dutch elm, gall, leaf soot, leaf curl, leaf spot (e.g. frog eye leaf spot, gray spot), powdery mildew (e.g. downy mildew, powdery mildew), Mold (e.g. white mold), mosaic virus, red root rot, wilt (e.g. clematis wilt, fusarium wilt, verticillium wilt), scab, red star, rhizoctoniasis (black spot), rice Rice blast, root rot, rust (e.g. Asian soybean rust), Fusarium head blight, soft rot, sclerotia, smut, stem rot, sudden death syndrome, galls (e.g. galls, potato rot) ), wet rot, white rot, white root (white silk), and yellow spot.

The strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure can be used to protect and enhance plant growth/yield under a variety of growing conditions, including Conditions include, but are not limited to, nutritional deficiencies (e.g., lack of calcium, iron, manganese, magnesium, nitrogen, phosphorus, potassium and/or sulfur), extreme humidity, extreme pH, extreme temperatures (e.g., 60, Average daytime temperature below 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74 or 75°C, 85, 86, 87, 88, 89, 90, 91 , 92, 93, 94, 95, 96, 97, 98, 99, 100°C or higher, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60 , 61, 62, 63, 64, 65, 66, 67, 68, 69 or nighttime average temperature below 70°C, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81 , 82, 83, 84, 85°C or more) and drought conditions (e.g. seasonal rainfall during the growing season of 5, 6, 7, 8, 9, 10, 11, 12, less than 13, 14 or 15 inches). Understand that any determination of what constitutes nutrient deficiencies, temperature extremes, drought conditions, etc. must consider the species/variety of the plant that is to be grown, as different species/cultivars may have different preferences and requirements. want to be

The strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure can be used to protect and enhance plant growth and/or yield in various geographic regions, such Geographic regions include, but are not limited to, Afghanistan, Argentina, Australia, Bangladesh, Bolivia, Brazil, Canada, Chile, China, Colombia, Ecuador, Egypt, Ethiopia, Europe (e.g. Austria, Belgium, Bulgaria, Czech Republic, Denmark). , France, Germany, Hungary, Ireland, Italy, Lithuania, Netherlands, Poland, Romania, Spain, Sweden and/or UK agricultural regions), India, Indonesia, Iran, Iraq, Japan, Kazakhstan, Kenya, Malawi, Mexico, Morocco , Nigeria, Pakistan, Paraguay, Peru, Philippines, Russia, South Africa, Taiwan, Tanzania, Thailand, Turkey, Ukraine, United States (e.g. Arkansas, Colorado, Idaho, Illinois, Indiana, Iowa, Kansas, agricultural regions of Kentucky, Michigan, Minnesota, Mississippi, Missouri, Montana, Nebraska, North Dakota, Ohio, Oklahoma, South Dakota, Texas and/or Wisconsin), Uzbekistan, Venezuela, Agricultural regions of Vietnam, Zambia and/or Zimbabwe may be mentioned. In some embodiments, one or more strains of the present disclosure (or inoculum compositions of the present disclosure) are used in multiple agricultural regions (e.g., Illinois, Iowa, Southern Minnesota and Eastern Nebraska). improve plant growth and/or yield in geographic areas, including agricultural areas of the United States. Examples of such geographic regions include, but are not limited to, northern corn regions including the agricultural regions of Iowa (e.g., northern Iowa), Michigan, Minnesota, North Dakota, South Dakota and/or Wisconsin. Illinois (e.g., Northern and/or Central Illinois), Indiana (e.g., Northern Indiana), Iowa (e.g., Southern Iowa), Kansas (e.g., Northern Kansas), Missouri (e.g., , Northern Missouri), Nebraska (e.g., Northern and/or Southern Nebraska) and/or Central Corn Territories including Ohio; Alabama (e.g., Northern and/or Southern Alabama), Arkansas, Georgia (e.g., , Northern and/or Southern Georgia), Illinois (e.g., Southern Illinois), Indiana (e.g., Southern Indiana), Kansas, Kentucky, Louisiana, Maryland, Missouri (e.g., Missouri) Central and/or Southern), Mississippi (e.g., Northern and/or Southern Mississippi), Nebraska (e.g., Southern Nebraska), North Carolina, Oklahoma, South Carolina, Tennessee, Texas and/or Southern Corn Territory, including agricultural areas of Virginia; including agricultural areas of Minnesota, Montana (e.g., Eastern Montana), Nebraska, North Dakota, South Dakota and/or Wyoming (e.g., Eastern Wyoming) northern wheat regions; northern wheat regions, including agricultural regions of Idaho, Oregon and/or Washington; central wheat regions, including agricultural regions of Colorado, Nebraska, South Dakota and/or Wyoming (e.g., eastern Wyoming) Territory; Central Wheat Region including agricultural areas of Illinois, Indiana, Iowa, Missouri and/or Ohio; Central Wheat Region including agricultural areas of Kansas, Oklahoma and/or Texas; and Oklahoma and and/or southern wheat regions, including agricultural regions of Texas.

The strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure can be used to kill, inhibit the growth and/or inhibit the reproduction/growth of a myriad of plant pests. can. In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or of the present disclosure are added to plants or plant parts (e.g., plant propagation material) or plant growth media (e.g., soil). Alternatively, application of an inoculum composition reduces about/at least 5, 10, 15, 20, 25, 30, 35, 40, 45 of one or more pests present on/in said plant, plant part or plant growth medium. , 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more. For example, in certain embodiments, E. billingiae NRRL B-67766, P. The application of P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769 or a cell-free filtrate or cell extract obtained therefrom) reduces the phytopathogenic fungi ( about/at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90 of phytopathogenic Zymoseptoria, Effective at killing 95% or more.

In certain embodiments, strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure into plants or plant parts (e.g., plant propagation material) or plant growth media (e.g., soil). Application of the above strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions may be applied to control plants, plant parts or plant growth media (e.g., untreated control plants and/or treated with another microbial strain). about/at least 5, 10, 15, 20 the growth of one or more pests present on/in said plant, plant part or plant growth medium compared to the growth of said pest on said plant, Effective at inhibiting by 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more. For example, in certain embodiments, E. billingiae NRRL B-67766, P. The application of P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769 or a cell-free filtrate or cell extract derived therefrom) is effective against one or more plant pathogens such as Inhibits growth of phytopathogenic Zymoseptoria by about/at least 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more effective for

In certain embodiments, application of strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure to plants or plant parts (e.g. plant propagation material) or plant growth media (e.g. soil) is of the plant, plant part or plant growth medium (e.g. untreated control plant and/or plant treated with another microbial strain) compared to reproduction/reproduction of said plant, plant part or plant growth medium about/at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, reproduction/reproduction of one or more pests present on/in Effective for 80, 85, 90, 95% or more inhibition. For example, in certain embodiments, E. billingiae NRRL B-67766, P. Application of P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769 (or a cell-free filtrate or cell extract obtained therefrom) can be used to kill one or more plant pathogens such as Growth/reproduction of phytopathogenic Zymoseptoria by about/at least 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more effective in suppressing

Thus, in certain embodiments, the methods and uses of the present disclosure include the addition of one or more one or more strains, cultures, cell-free filtrates, cell extracts of the present disclosure in amounts/concentrations effective to kill, inhibit the growth of and/or inhibit the reproduction/reproduction of plant pests of comprising, consisting essentially of, or consisting of applying a product and/or an inoculum composition.
In certain embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure are added to one or more control plants (e.g., plants grown from untreated plants, and / or plants grown from seeds treated with a control composition that is identical to the inoculum composition of the present disclosure except that it lacks at least one of the strains of the present disclosure found in the inoculum composition). , 1, 2, 3, 4, 5 or more of disease severity (e.g., disease incidence, area affected, pathogen load, infection/prevalence rate, symptom severity and/or toxin load) Effective in reducing properties by at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more amount/concentration applied to seeds.

Strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure can be used for, but are not limited to, disease incidence, diseased area, pathogen load, rate of infection/spread, symptom severity and/or Or it can be used to reduce many aspects of disease severity, including toxin load. In certain embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure to plants or plant parts (e.g. plant propagation material) or plant growth media (e.g. soil) is used to reduce one or more aspects of disease severity (e.g. disease incidence, area affected, pathogen load, infection/prevalence rate, severity of symptoms and/or toxin load) by about/at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more. For example, in certain embodiments, E. billingiae NRRL B-67766, P. Application of P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769 or a cell-free filtrate or cell extract obtained therefrom) is mediated by a fungal infection (e.g. Zymoseptoria). disease incidence, area affected, pathogen load, infection/prevalence rate, severity of symptoms and/or toxin load in untreated controls and/or plants treated with another microbial strain. About/at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% compared to those of fungal infections Or decrease more.

Thus, in certain embodiments, the methods and uses of the present disclosure provide seeds, plant growth media on which said seeds are growing or will be grown, and/or plants growing from said seeds to determine disease severity. applying one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure in amounts/concentrations effective to reduce one or more properties of , consisting essentially of or consisting of

In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and inoculum compositions of the present disclosure are added to one or more control plants (e.g., plants grown from untreated seeds and /or plants grown from seeds treated with a control composition identical to the inoculum composition of the present disclosure but lacking at least one of the strains of the present disclosure found in the inoculum composition of the present disclosure) is applied to the seeds in an amount/concentration effective to reduce the

In some embodiments, one or more of the strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure reduce disease severity in seed-grown plants of 1, 2, 3, 4. Five or more characteristics (e.g. disease incidence, diseased area, pathogen load, infection/prevalence rate, symptom severity and/or toxin load) were compared to one or more control plants (e.g. untreated and/or seeds treated with a control composition identical to the inoculum composition of the present disclosure but lacking at least one of the strains of the present disclosure found in the inoculum composition of the present disclosure plants) at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more effective in reducing

In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure reduce the disease severity of plants growing in plant growth medium to 1, 2 , 3, 4, 5 or more characteristics (e.g. disease incidence, diseased area, pathogen load, infection/prevalence rate, symptom severity and/or toxin load) compared to one or more controls (e.g. at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, compared to plants grown in untreated soil and/or soil treated with alternative microbial strains) Amounts/concentrations effective to reduce by 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200, 225, 250% or more is introduced into the plant growth medium (eg, soil) at .

In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure are added to one or more controls (e.g., untreated plants and/or alternative about/at least 5, 10, 15, 20, 25, 30, 35, 1, 2, 3, 4, 5 or more growth and/or yield characteristics compared to plants treated with the microbial strain; , 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200, 225, 250% or more Improve. For example, in some embodiments, E. billingiae NRRL B-67766, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769 (or The resulting acellular filtrate or cell extract) yields about/at least 0.25, 0.5, 0.75, 1, 1.1, 1.2, 1.3, 1.4, 11.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, Improves grain or pseudograin yield by 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5 or 4.6 bushels/acre. Similarly, in some embodiments, E. billingiae NRRL B-67769, P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769 (or a cell-free filtrate or cell extract obtained therefrom) Application reduced the yield of legume plants by about/at least 0.25, 0.5, 0.75, 1, 1.1, 1.2, 1, 1.1, 1.2, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 2, 3, 4, 5, 7, 8, 9, 10 compared to the yield of untreated control plants and/or plants treated with the alternative microbial strains. Improve by 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9 or 2.0 bushels/acre.

Thus, in certain embodiments, the methods and uses of the present disclosure are applied to the seed of a cereal, pseudocereal or legume plant growing or to grow said cereal, pseudocereal or legume seed. an amount effective to enhance one or more plant growth characteristics and/or one or more plant yield characteristics in the medium and/or in plants grown from said cereal, pseudocereal or legume seeds/ Including, consisting essentially of, or consisting of applying, at a concentration.

In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure are grown from one or more control plants (e.g., untreated seeds). and/or seeds treated with a control composition identical to the inoculum composition of the present disclosure but lacking at least one of the strains of the present disclosure found in the inoculum composition of the present disclosure. 1, 2, 3, 4, 5 or more plant growth characteristics (e.g. biomass) and/or 1, 2, 3, 4, 5 or more plant yields of said seed-grown plants compared to plants) volume characteristics (e.g. bushels/acre) of at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100 , 105, 110, 115, 120, 125, 150, 175, 200, 225, 250% or more. According to some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure have a yield of about/at least 0.25, 0.5, 0.75, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4, 3.5, of grains or pseudograins in an amount effective to enhance 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5 or more bushels per acre Applied to seeds. According to some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure have a yield of about/at least 0.25, 0.5, 0.75, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3 or more bushels/acre is applied to the legume seeds in an amount effective for

In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure are added to one or more control plants (e.g., untreated plants and/or from seeds compared to plants treated with a control composition that is identical to the inoculation composition of the disclosure except that it lacks at least one of the strains of the disclosure found in the inoculum composition of the disclosure. 1, 2, 3, 4, 5 or more plant growth characteristics (e.g., biomass) and/or 1, 2, 3, 4, 5 or more plant yield characteristics (e.g., bushels/acre) of growing plants at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, It is applied to plants in an amount/concentration effective to provide 150, 175, 200, 225, 250% or more improvement. According to some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure have a yield of about/at least 0.25, 0.5, 0.75, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, to the grain or pseudograin in an amount effective to enhance 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5 or more bushels per acre applied. In some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure have a yield of about/at least 0.25, 0.5, 0.75, 1, 1.1 , 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3 or more bushels/acre.

In some embodiments, application of one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure is applied to one or more controls (e.g., grown in untreated soil). 1, 2, 3, 4, 5 or more plant growth characteristics (e.g., biomass) and/or 4, 5 or more plant yield characteristics (e.g. bushels/acre) of at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80 , 85, 90, 95, 100, 105, 110, 115, 120, 125, 150, 175, 200, 225, 250% or more. According to certain embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure are about/at least 0.25, 0.5, 0.75, 1, 1.1, 1.2, 1.3 , 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8 , 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5 or more bushels/acre to improve grain or pseudo-grain yield. According to some embodiments, one or more strains, cultures, cell-free filtrates, cell extracts and/or inoculum compositions of the present disclosure are about/at least 0.25, 0.5, 0.75, 1, 1.1, 1.2 , 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3 or more bushels per acre is introduced into the plant growth medium in an amount effective to induce

Certain embodiments of the disclosure are described in the following numbered items:
[1] Optionally, E. billingiae NRRL B-67766 or a closely related strain thereof (e.g., a closely related progeny of E. billingiae NRRL B-67766 or a closely related strain derived from E. billingiae NRRL B-67766). modified microbial strains), P. gessardii NRRL B-67767 or closely related strains thereof (e.g., closely related descendants of P. gessardii NRRL B-67767 or closely related strains derived from P. gessardii NRRL B-67767) or modified microbial strains of P. libanesis NRRL B-67769 or closely related strains thereof (e.g., closely related descendants of P. libanesis NRRL B-67769 or closely related strains derived from P. libanesis NRRL B-67769). The isolate of the present disclosure, which is a modified microbial strain of

[2] E. billingiae NRRL B-67766 or a closely related strain thereof (e.g., a closely related descendant of E. billingiae NRRL B-67766 or a closely related modified microbial strain derived from E. billingiae NRRL B-67766) ) biologically pure cultures.
[3] P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., a closely related descendant of P. gessardii NRRL B-67767 or a closely related modified microbial strain derived from P. gessardii NRRL B-67767 ) biologically pure cultures.
[4] P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related descendant of P. libanesis NRRL B-67769 or a closely related modified microbial strain derived from P. libanesis NRRL B-67769) ) biologically pure cultures.

[5] one or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g., closely related progeny of E. billingiae NRRL B-67766 or E. billingiae NRRL) A closely related modified microbial strain derived from B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., a closely related progeny of P. gessardii NRRL B-67767 or P. gessardii NRRL B -67767), or P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related progeny of P. libanesis NRRL B-67769 or P. libanesis NRRL B) A complete broth culture containing a closely related modified microbial strain derived from -67769).
[6] one or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g., closely related progeny of E. billingiae NRRL B-67766 or E. billingiae NRRL) A closely related modified microbial strain derived from B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., a closely related progeny of P. gessardii NRRL B-67767 or P. gessardii NRRL B -67767), or P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related progeny of P. libanesis NRRL B-67769 or P. libanesis NRRL B) A cell-free filtrate derived from a culture containing a closely related modified microbial strain derived from -67769).

[7] E. billingiae NRRL B-67766 or a closely related strain thereof (e.g., a closely related descendant of E. billingiae NRRL B-67766 or a closely related modified microbial strain derived from E. billingiae NRRL B-67766) ), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., a closely related descendant of P. gessardii NRRL B-67767 or a closely related modified microbial strain derived from P. gessardii NRRL B-67767) , or P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related descendant of P. libanesis NRRL B-67769 or a closely related modified microbial strain derived from P. libanesis NRRL B-67769) A cell extract obtained from a culture containing
[8] one or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g., closely related descendants of E. billingiae NRRL B-67766 or derived from E. billingiae NRRL B-67766) a closely related modified microbial strain derived from P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., a closely related descendant of P. gessardii NRRL B-67767 or a closely related modified microbial strain derived from P. gessardii NRRL B-67767) strain), or P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related descendant of P. libanesis NRRL B-67769 or a closely related modified microbial strain derived from P. libanesis NRRL B-67769) An inoculum composition comprising, consisting essentially of, or consisting of a legally acceptable carrier.

[9] The composition contains one or more strains of the present disclosure at about 1×10 ³ to about 1×10 ¹² colonies per gram (g) and/or milliliter (mL) of the inoculum composition (respectively). Forming Units (cfu), optionally about/at least 1 x 103, 1 x ¹⁰⁴ , 1 x 105, 1 x ¹⁰⁶ , ¹ x ^{107 ,} 1 x ¹⁰⁸ , 1 x ¹⁰⁹ , 1 9. The inoculum composition of item 8, comprising ^x1010 , 1 x 1011, or ¹ x ¹⁰¹² cfu.
[10] The inoculating composition according to any one of items 8-9, wherein said composition further comprises one or more stabilizing compounds.

[11] The one or more stabilizing compounds are
one or more monosaccharides, optionally arabinose, fructose and/or glucose;
one or more disaccharides, optionally maltose, sucrose and/or trehalose;
one or more maltodextrins, optionally, for example one or more maltodextrins having a DEV value (each and/or collectively) of about 15 to about 20;
one or more sugar alcohols, optionally arabitol, mannitol, sorbitol and/or xylitol;
one or more humic acids, optionally potassium humate and/or sodium humate;
one or more fulvic acids, optionally potassium fulvicate and/or sodium fulvicate;
one or more hygroscopic polymers, optionally one or more of albumins, alginates, celluloses, gums (e.g. cellulose gum, guar gum, gum arabic, combretum gum, xanthan gum), methylcellulose, nylon, pectin, polyacrylic acid, polycarbonate , polyethylene glycol (PEG), polyethyleneimine (PEI), polylactide, polymethylacrylate (PMA), polyurethane, polyvinyl alcohol (PVA), polyvinylpyrrolidone (PVP), propylene glycol, sodium carboxymethylcellulose and/or starch;
one or more oxidation control ingredients, optionally one or more antioxidants (e.g., ascorbic acid, ascorbyl palmitate, ascorbyl stearate, calcium ascorbate, one or more carotenoids, lipoic acid, one or more phenolic compounds (e.g., one or more flavonoids, flavones and/or flavonols), potassium ascorbate, sodium ascorbate, one or more thiols (e.g., glutathione, lipoic acid and/or N-acetylcysteine), one tocopherols, one or more tocotrienols, ubiquinones and/or uric acid) and/or one or more oxygen scavengers, optionally ascorbic acid and/or sodium bicarbonate; and/or one or more UV protection 11. An inoculum composition according to item 10, comprising, consisting essentially of, or consisting of an agent, optionally one or more lignosulfites.

[12] The one or more stabilizing compounds comprise from about 0.0001 to about 10% by weight of the composition, optionally from about 2 to about 6% by weight of the composition, optionally from about 0.0005, 0.001, 0.002, 0.003, 0.004, 0.005, 0.0075, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.45 12. Any one of items 10 to 11, comprising 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 7, 7.5, 8, 8.5, 9, 9.5 or 10% by weight. inoculation composition.

[13] said one or more stabilizing compounds are
0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0, 5, 10, 15, 20, 25, 30, 35 , 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity of 1, 2, 3, 4, 5, 6, 7, 8, 9 , 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64 , 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer;
drying up to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more;
Dry to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and 0, 1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 , 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 at 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity , 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72 , 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer;
1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 at -80°C or below , 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer cryopreservation;
application to plant propagation material (optionally seeds);
Application to plant propagation material and about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90%, 95% or more drying to;
Application to plant propagation material and 0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21 , 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0, 5, 10, 15, 1, 2, 3, 4, 5, 6 at 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity , 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52 , 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer;
foliar spray;
foliar application and drying to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; and/or foliar application and 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 , 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 0.1, 0.2, 0.25, 0.5 to relative humidity of 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more, 0.75, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, present in an amount/concentration sufficient to ensure that said one or more strains of the present disclosure remain viable in the inoculum composition of the present disclosure after exposure for a period of 21 days or more. 13. The inoculation composition according to any one of 10-12.

[14] The one or more stabilizing compounds are
0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0, 5, 10, 15, 20, 25, 30, 35 , 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity of 1, 2, 3, 4, 5, 6, 7, 8, 9 , 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64 , 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer;
drying up to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more;
Dry to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and 0, 1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 , 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 at 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity , 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72 , 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer;
1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 at -80°C or below , 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer cryopreservation;
application to plant propagation material (optionally seeds);
Application to plant propagation material and up to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more drying;
Application to plant propagation material and 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 , 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0, 5, 10, 15, 1, 2, 3, 4, 5, 6 at 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity , 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52 , 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer;
foliar spray;
foliar application and drying to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; and/or foliar application and 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 , 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 0.1, 0.2, 0.25, 0.5 to relative humidity of 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more, 0.75, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, at least 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 25, of one or more strains of the present disclosure after exposure for a period of 21 days or more; Sufficient amount/ 13. An inoculum composition according to any one of items 10-12, present in a concentration.

[15] The stabilizing compound is
0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0, 5, 10, 15, 20, 25, 30, 35 , 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity of 1, 2, 3, 4, 5, 6, 7, 8, 9 , 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64 , 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer;
drying up to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more;
Dry to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more and 0, 1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 , 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0, 5, 10, 15, 20, 25, 30, 35, 40, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 at 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity , 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72 , 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer;
1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 at -80°C or below , 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer cryopreservation;
application to plant propagation material (optionally seeds);
Application to plant propagation material and about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90%, 95% or more drying to;
Application to plant propagation material and 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 , 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40°C and 0, 5, 10, 15, 1, 2, 3, 4, 5, 6 at 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more relative humidity , 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52 , 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or longer;
foliar spray;
foliar application and drying to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more; and/or foliar application and 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 , 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and/or 40° C. and 0, 5, 10, 0.1, 0.2, 0.25, 0.5 to relative humidity of 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95% or more, 0.75, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, At least 1 x 101, 1 x 102, 1 x 103, 1 x 104, 1 x 105, 1 x 105, 1 x 105, 1 x 103, 1 x 104, 1 x 105, 1 x 103, 1 x 104, ¹ x 105, ¹ x 103, ¹ x 104 ^{, 1} x 105 strain of the one or more strains of the present disclosure after exposure for a period of 21 days or more Sufficient to ensure that x10 ⁶ , 1 x 10 ⁷ , 1 x 10 ⁸ , 1 x 10 ⁹ , 1 x 10 ¹⁰ or more colony forming units (cfu) remain viable in the inoculum composition. 13. An inoculum composition according to any one of items 10-12, present in an amount/concentration.

[16] The inoculation composition of any one of items 8-15, wherein said composition further comprises one or more biostimulants, optionally one or more seaweed extracts, myoinositol and/or glycine. thing.
[17] The inoculum composition of any one of items 8-16, wherein said composition further comprises one or more microbial extracts, optionally one or more of the microbial extracts specifically disclosed above. .
[18] The composition contains one or more nutrients, optionally one or more vitamins (e.g., vitamin _A , vitamin _B complex (i.e., vitamin B1, vitamin B2, vitamin B3 _, vitamin B) ₅ , vitamin _B6 , vitamin _B7 , vitamin _B8 , vitamin _B9 , vitamin _B12 , and/or choline), vitamin C, vitamin D, vitamin E, vitamin K), carotenoids (α-carotene, β- carotenes, cryptoxanthin, lutein, lycopene and/or zeaxanthin), macrominerals (e.g. calcium, iron, magnesium, nitrogen, phosphorus, potassium and/or sodium), trace elements (e.g. boron, cobalt, chloride, chromium, copper, fluoride, iodine, iron, manganese, molybdenum, selenium and/or zinc) and/or organic acids (e.g. acetic acid, citric acid, lactic acid, malic acid and/or taurine) of items 8-17. An inoculum composition according to any one of the preceding claims.

[19] The composition comprises one or more pest attractants and/or contact irritants, optionally brevicomin, ceralua, codrelua, cuelua, disparua, dominicalua, eugenol, frontalin, gossiplua, groundlua, hexalua , ipsdienol, ipsenol, japonylua, lachitrua, lineatin, litrua, rupulua, medlua, mega tomoic acid, methyl eugenol, moguchun, α-multistriatin, muscarua, orfuralua, orictalua, ostramon, rescalure, cigurua , sulcatol, trimedlua and/or trunkol.

[20] The composition optionally comprises:
one or more fungicides, optionally one or more of the fungicides explicitly disclosed above;
one or more herbicides, optionally one or more of the herbicides specifically disclosed above;
one or more insecticides, optionally one or more of the insecticides specifically disclosed above; and/or one or more nematicides, optionally one of the herbicides specifically disclosed above. 20. The inoculation composition of any one of items 8-19, further comprising more than one seed.

[21] The inoculating composition according to any one of items 8-20, wherein said composition further comprises one or more lipochitooligosaccharides, optionally lipochitooligosaccharides represented by formulas I-IV.
[22] The inoculating composition according to any one of items 8-20, wherein said composition further comprises one or more lipochitooligosaccharides represented by structures V-XXXIII.
[23] The inoculum of any one of items 8-22, wherein the composition further comprises one or more chitooligosaccharides, optionally one or more chitin oligosaccharides represented by formulas XXXIV-XXXV. Composition.
[24] The inoculating composition according to any one of items 8-22, wherein the composition further comprises one or more chitin oligosaccharides represented by structures XXXV-LXXXIII.
[25] The inoculation composition according to any one of items 8 to 22, wherein the composition further comprises one or more chitinous compounds, optionally one or more chitins and/or one or more chitosans. thing.

[26] The composition comprises one or more flavonoids, optionally one or more anthocyanidins, such as cyanidin, delphinidin, malvidin, pelargonidin, peonidin and/or petunidin; , apigenin, baicalein, chrysin, 7,8-dihydroxyflavone, diosmin, flavoxate, 6-hydroxyflavone, luteolin, scutellarein, tangeritin and/or ougonin) and/or flavonols (e.g. amlensin, astragalin, azaleatin, azalein, fisetin, furanoflavonol galangin, gosypetin, 3-hydroxyflavone, hyperoside, icariin, isoquercetin, kaemperide, kaemperithrin, kaempferol, isorhamnetin, morin, myricetin, myricitrin, natsudaidyne, pachypodol, pyranoflavonol quercetin, quercetin, rhamnadin, ramnetin , robinin, rutin, spireoside, troxerutin and/or xanthoramnin); flavanones such as butin, eriodictyol, hesperetin, hesperidin, homoeriodictyol, isosakuranetin, naringenin, naringin, pinocembrin, poncilin, sakuranetin, sakuranin and/or sterbin flavanonols such as dihydrokaempferol and/or taxifolin; flavans such as flavan-3-ols (e.g. catechin (C), catechin 3-gallate (Cg), epicatechin (EC), epigallocatechin (EGC ), 3-epicatechin gallate (ECg), 3-epigallocatechin gallate (EGCg), epiafzelekin, fisetinidol, gallocatechin (GC), 3-gallocatechin gallate (GCg), guibourtinidol, mesquitol, robinetinidol, theaflavin 3-gallate, theaflavin 3′-gallate, theaflavin 3,3′-digallate, thearubigin), flavan-4-ols (e.g. apiforol and/or luteofol) and/or flavan-3,4 - diols (e.g. leucocyanidin, leucodelphinidin, leucofisetinidin, leucomalvidin, leucopelargonidin, leucopeonidin, leucorobinetinidin, melakacidin and/or terakashi and/or isoflavonoids, such as isoflavones (e.g., biochanin A, daidzein, formononetin, genistein and/or glycitein), isoflavans (e.g., equol, roncocarpan and/or laxiflorane, isoflavandiol, isoflavenes (e.g. glabrene, haginin D and/or 2-methoxyudaicin), coumestans (e.g. coumestrol, plikadin and/or wederolactone), pterocarpanes and/or retinoids; and/or one thereof One or more analogues, derivatives, hydrates, isomers, polymers, salts and solvates such as neoflavonoids (e.g. Calophylloride, Ctareagenin, Dalverdichromene, Dalverdin, Nibetin) and Pterocarpane (e.g. Bitsucarpine A, viccarpine B, eribraedin A, eribraedin B, erythrabicin II, erythrabicin-1, erychrystagalin, glycinol, glyceolysin, glyceoline, glycyrrhizol, marquiain, medicarpine, molycyanin, orientanol, phaseolin, pisatine, striatine and/or trifolilysine) 26. The inoculation composition of any one of items 8-25, further comprising

[27] The inoculation composition according to any one of items 8-26, wherein said composition further comprises jasmonic acid and/or one or more derivatives thereof.
[28] The inoculating composition according to any one of items 8-27, wherein said composition further comprises linoleic acid and/or one or more derivatives thereof.
[29] The inoculating composition according to any one of items 8-28, wherein said composition further comprises linolenic acid and/or one or more derivatives thereof.
[30] The inoculation composition of any one of items 8-29, wherein said composition further comprises one or more karrikin, optionally one or more karrikin represented by formula LXXXIV.
[31] The inoculation composition according to any one of items 8 to 30, wherein the composition further comprises gluconolactone.
[32] The inoculum composition of any one of items 8-31, wherein said composition further comprises one or more additional microorganisms.
[33] said one or more additional microorganisms comprise or essentially comprise one or more microorganisms that improve soil nutrient availability, optionally diazotrophs and/or phosphate solubilizing microorganisms 33. An inoculating composition according to item 32, essentially consisting of or consisting of.

[34] The one or more additional microorganisms are Azospirillum brasilense INTA Az-39, Azospirillum brasilense AZ39, Azospirillum brasilense XOH, Azospirillum brasilense (Azospirillum brasilense) BR 11002, Azospirillum brasilense BR 11005, Azospirillum brasilense Ab-V5, Azospirillum brasilense Ab-V6, Bacillus amyloliquefaciens amyloliquefaciens D747, Bacillus amyloliquefaciens NRRL B-50349, Bacillus amyloliquefaciens TJ1000, Bacillus amyloliquefaciens FZB24, Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens FZB42, Bacillus amyloliquefaciens IN937a, Bacillus amyloliquefaciens IT-45, Bacillus amyloliquefaciens MBI 600, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) BS27 (deposited as NRRL B-5015), Bacillus amyloliquefaciens BS2084 (deposited as NRRL B-50013), Bacillus amyloliquefaciens 15AP4 (deposited as NRRL B-50013) (deposited as ATCC PTA-6507), Bacillus amyloliquefaciens iquefaciens 3AP4 (deposited as ATCC PTA-6506), Bacillus amyloliquefaciens LSSA01 (deposited as NRRL B-50104), Bacillus amyloliquefaciens ABP278 (NRRL B -50634), Bacillus amyloliquefaciens 1013 (deposited as NRRL B-50509), Bacillus amyloliquefaciens 918 (deposited as NRRL B-50508) ), Bacillus amyloliquefaciens 22CP1 (deposited as ATCC PTA-6508), and Bacillus amyloliquefaciens BS18 (deposited as NRRL B-50633), Bacillus cereus (Bacillus cereus) (Bacillus cereus) I-1562, Bacillus firmus I-1582, Bacillus liqueniformis (Licheniformis) BA842 (deposited as NRRL B-50516), Bacillus licheniformis (Bacillus liqueniformis) BL21 (deposited as NRRL B-50134), Bacillus megaterium Q62UKE (deposited as NRRL B-67352), Bacillus megaterium O723G4 (deposited as NRRL B-67357) Bacillus megaterium O132FX (deposited as NRRL B-67521), Bacillus megaterium O14VDF (deposited as NRRL B-67522), Bacillus megaterium O249FV (deposited as NRRL B-67523), Bacillus mega Bacillus megaterium O33Y38 (deposited as NRRL B-67524), Bacillus megaterium O62RUA (deposited as NRRL B-67525), Bacillus megaterium O62RV8 (deposited as NRRL B-67526) Bacillus megaterium O62STA (deposited as NRRL B-67527), Bacillus megaterium O7237E (deposited as NRRL B-67528), Bacillus megaterium ) O72NWX (deposited as NRRL B-67529), Bacillus megaterium O72T97 (deposited as NRRL B-67530), Bacillus megaterium NRRL B-67533, Bacillus megaterium ) NRRL B-67534, Bacillus mycoides NRRL B-21664, Bacillus pumilus NRRL B-21662, Bacillus pumilus NRRL B-30087, Bacillus pumilus ATCC 55608, Bacillus pumilus ATCC 55609, Bacillus pumilus GB34, Bacillus pumilus KFP9F, Bacillus pumilus QST 2808, Bacillus subtilis (Bacillus subtilis) ) ATCC 55078, Bacillus subtilis ATCC 55079, Bacillus subtilis MBI 600, Bacillus subtilis NRRL B-21661, Bacillus subtilis NRRL B-21665, Bacillus subtilis CX-9060, Bacillus subtilis GB03, Bacillus subtilis GB07, Bacillus subtilis QST-713, Bacillus subtilis subtilis FZB24, Bacillus subtilis D747, Bacillus subtilis 3BP5 (deposited as NRRL B-50510), Bacillus thringiensis ATCC 13367, Bacillus thuringiensis thringiensis GC-91, Bacillus thringiensis NRRL B-21619, Bacillus thringiensis ABTS-1857, Bacillus thringiensis SAN 401 I, Bacillus thringiensis ) ABG-6305, Bacillus thringiensis ABG-6346, Bacillus thringiensis AM65-52, Bacillus thringiensis SA-12, Bacillus thringiensis SB4 , Bacillus thringiensis ABTS-351, Bacillus thringiensis HD-1, Bacillus thringiensis EG 2348, Bacillus thringiensis EG 7826, Bacillus thringiensis Bacillus thringiensis EG 7841, Bacillus thringiensis DSM 2803, Bacillus thringiensis Bacillus thringiensis NB-125, Bacillus thringiensis NB-176, BRADY, Lysinibacillus sphaericus O62Q8F (deposited as NRRL B-67350), Lysinibacillus sphaericus Lysinibacillus sphaericus O62S63 (deposited as NRRL B-67351), Lysinibacillus sphaericus O62QG9 (deposited as NRRL B-67486), Mesorhizobium huakii LL32, Pseudomonas jeseni （Pseudomonas jessenii）PS06、リゾビウム・レグミノサルム（Rhizobium leguminosarum）162BB1、リゾビウム・レグミノサルム（Rhizobium leguminosarum）162P17、リゾビウム・レグミノサルム（Rhizobium leguminosarum）175G10b、リゾビウム・レグミノサルム（Rhizobium leguminosarum）D36、リゾビウム・レグミノサルム（Rhizobium leguminosarum）SO12A -2 (IDAC 080305-01), Rhizobium loti 95C11, Rhizobium loti 95C14, Sinorhizobium fredii CCBAU114, Sinorhizobium fredii USDA 205, Sinorhizobium meliloti Sinorhizobium meliloti 102F34a Sinorhizobium meliloti 102F51a Sinorhizobium meliloti 102F77b Sinorhizobium meliloti B401 yces) NRRL B-30145, Streptomyces M1064, Streptomyces WYE 53 (deposited as ATCC 55750), Streptomyces lydicus WYEC 108 (deposited as ATCC 55445) ), Yersinia entomophaga MH96 (also known as O43NEW) (deposited as DSM 18238, DSM 22339, ATCC BAA-1678 and NRRL B-67598), Yersinia entomophaga O82KB8, Yersinia entomophaga O82KB8, Yersinia entomophaga Yersinia entomophaga O24G3R (deposited as NRRL B-67599), Yersinia entomophaga O24KEK (deposited as NRRL-67600), Yersinia entomophaga O333A4 (deposited as NRRL B-67601) in item 32 comprising, consisting essentially of or consisting of Yersinia entomophaga O23ZMJ, Yersinia entomophaga O348UX and/or Yersinia entomophaga O33ZDX The inoculum composition as described.

[35] the one or more additional microorganisms are Gliocladium virens ATCC 52045, Gliocladium virens GL-21, Glomus intraradices RTI-801 , Metarhizium anisopliae F52, PENI, Trichoderma asperellum SKT-1, Trichoderma asperellum ICC 012, Trichoderma atroviride LC52, Trichoderma atroviride CNCM 1-1237, Trichoderma fertile JM41R, Trichoderma gamsii ICC 080, Trichoderma hamatum ATCC 52198, Trichoderma harzianum ATCC 52445, Trichoderma harzianum KRL-AG2, Trichoderma harzianum T-22, Trichoderma harzianum TH-35, Trichoderma harzianum T-39, Trichoderma harzianum ICC012, Trichoderma reesei ( Trichoderma reesei ATCC 28217, Trichoderma virens ATCC 58678, Trichoderma virens GI-3, Trichoderma virens GL-21, Trichoderma virens G-41, Trichoderma・Trichoderma viridae ATCC 52440, Trichoderma viridae (Trich 33. An inoculum composition according to item 32, comprising, consisting essentially of or consisting of Trichoderma viridae ICC080, and/or Trichoderma viridae TV1.

[36] The one or more additional microorganisms comprise one or more biopesticides, optionally one or more acaricidal, insecticidal and/or nematicidal microorganisms and one or more 33. The inoculum composition of item 32, comprising, consisting essentially of, or consisting of a fungal microorganism.
[37] said composition comprises from about 1 x 103 to about ¹ x ¹⁰¹² colony forming units (cfu) per gram (g) and/or milliliter (mL) of inoculating composition of said one or more additional Microorganisms, optionally about/at least 1 x 103, 1 x 104, 1 x 105, 1 x ¹⁰⁶ , ¹ x ^{10 per} gram (g) and/or milliliter (mL) of inoculum composition 37. Any one of items 32-36, comprising ⁷ , 1×10 ⁸ , 1×10 ⁹ , 1×10 ¹⁰ , 1×10 ¹¹ , or 1×10 ¹² cfu of said one or more additional microorganisms. inoculum composition.

[38] The inoculating composition according to any one of items 8-37, wherein said composition is non-aqueous.
[39] The inoculating composition according to any one of items 8-37, wherein said composition is aqueous.
[40] The composition comprises: less than 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75 or 5% ( 38. An inoculum composition according to any one of items 8 to 37, comprising: by weight, based on the total weight of the composition.

[41] The inoculation composition according to any one of items 8-40, wherein the composition is an amorphous liquid.
[42] An inoculum composition according to any one of items 8-40, wherein said composition is an amorphous solid.
[43] Any one of items 8 to 40, wherein said composition is a freeze-, spray-, spray-freeze-dried composition, optionally a freeze-, spray- or spray-freeze-dried powder. Inoculum composition.

[44] A non-natural composition comprising the isolate of item 1 and a plant or plant part to which the isolate of item 1 has been applied.
[45] combining the biologically pure culture of any one of items 2-4 with a plant or plant part to which the biologically pure culture of any one of items 2-4 has been applied; Non-natural compositions, including
[46] A non-natural composition comprising the complete broth culture of item 5 and a plant or plant part to which the complete broth culture of item 5 has been applied.
[47] A non-natural composition comprising the cell-free filtrate of item 6 and a plant or plant part to which the cell-free filtrate of item 6 has been applied.
[48] A non-natural composition comprising the cell extract of item 7 and a plant or plant part to which the cell extract of item 7 has been applied.
[49] A non-natural composition comprising the inoculation composition according to any one of items 8-43 and a plant or plant part to which the inoculation composition according to any one of items 8-43 has been applied.

[50] A plant propagation material, optionally a seed; and a coating covering at least a portion of the outer surface of said plant propagation material, the isolate of item 1, any one of items 2-4. comprising or essentially a biologically pure culture, the complete broth culture of item 5, the cell-free filtrate of item 6, the cell extract of item 7 or the inoculum composition of any one of items 8-43. 50. A non-natural composition according to any one of items 44 to 49, comprising, consisting essentially of or consisting of: a coating consisting essentially of or consisting of;
[51] The coating comprises one or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or a closely related strain thereof (e.g., a closely related progeny of E. billingiae NRRL B-67766 or E. billingiae). closely related modified microbial strains derived from NRRL B-67766), P. gessardii NRRL B-67767 or closely related strains thereof (e.g., closely related progeny of P. gessardii NRRL B-67767 or derived from P. gessardii NRRL B-67767) or a closely related modified microbial strain of P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related descendant of P. libanesis NRRL B-67769 or a closely related modification derived from P. libanesis NRRL B-67769) 51. The non-natural composition of item 50, comprising an outer coating layer lacking (or essentially lacking) said one or more strains of the present disclosure.

[52] The coating comprises one or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or a closely related strain thereof (e.g., a closely related progeny of E. billingiae NRRL B-67766 or E. billingiae). closely related modified microbial strains derived from NRRL B-67766), P. gessardii NRRL B-67767 or closely related strains thereof (e.g., closely related progeny of P. gessardii NRRL B-67767 or derived from P. gessardii NRRL B-67767) or a closely related modified microbial strain of P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related descendant of P. libanesis NRRL B-67769 or a closely related modification derived from P. libanesis NRRL B-67769) from about 1 x 10 ¹ to about 1 x 10 ¹⁵ colony forming units (cfu), optionally 1 x 10 ⁴ , 1 x 10 ⁵ , 1 x 10 ⁶ , 1 x 10 ⁷ (each) 52. The non-natural composition of any one of items 50-51, comprising or more colony forming units.

[53] A plant germinated from the plant part according to any one of items 44-52.
[54] A plant part harvested from the plant according to any one of items 44-53.
[55] Processed products made from the plant parts of item 54.
[56] A crop comprising, consisting essentially of, or consisting of a plurality of plants according to any one of items 44-53.
[57] A kit comprising: the plant or plant part according to any one of items 44 to 54; and a container containing the plant or plant part.

[58] reduce the amount of ambient light reaching the coated plant propagation material when the container is sealed to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 , 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100%.
[59] When said container is sealed, reduce the amount of ambient oxygen reaching said plant propagation material to about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 , 65, 70, 75, 80, 85, 90, 95 or 100%.
[60] about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 when the container is sealed; 60, 65, 70 or 75%.

[61] the container is about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 225, 250; Containing or consisting essentially of a material having an oxygen permeability of less than 275, 300, 325, 350, 375, 400, 425, 450, 475, or 500 ^cm3 / ^m2 ·day (measured according to ASTM D3985) 61. A kit according to any one of items 57-60, which is or consists of.
[62] The kit further comprises one or more oxygen absorbing compounds, optionally activated carbon, iron powder, sodium chloride, ferrous carbonate, one or more metal halide catalysts and/or sodium bicarbonate. 62. The kit of any one of items 57-61, comprising:
[63] The isolate of item 1, the biologically pure culture of any one of items 2-4, the complete broth culture of item 5, the cell-free filtrate of item 6, the cell extract of item 7 , or an inoculum composition according to any one of items 8 to 43, to the plant or plant part, optionally to the seed. .

[64] said isolate, biologically pure culture, complete broth culture, cell-free filtrate, cell extract or inoculum composition is one or more fungi in/on/on the plant or plant part kill pests, inhibit the growth of one or more fungal pests, inhibit the reproduction/growth of one or more fungal pests, and/or reduce the severity of one or more fungal diseases. 64. The method of item 63, wherein the method is applied to said plant or plant part in an amount/concentration effective for
[65] whether said isolate, biologically pure culture, complete broth culture, cell-free filtrate, cell extract or inoculum composition enhances the growth and/or yield of said plant or plant part; 64. The method of item 63, wherein the method is applied to said plant or plant part in an amount/concentration effective for
[66] The isolate of item 1, the biologically pure culture of any one of items 2-4, the complete broth culture of item 5, the cell-free filtrate of item 6, the cell extract of item 7. , or an inoculum composition according to any one of items 8 to 43 in plant propagation material, optionally in seeds, said plant propagation material in a plant growth medium (optionally soil). Sometimes a method comprising, consisting essentially of, or consisting of applying.

[67] The isolate of item 1, the biologically pure culture of any one of items 2-4, the complete broth culture of item 5, the cell-free filtrate of item 6, the cell extract of item 7. , or an inoculum composition according to any one of items 8 to 43 in plant propagation material, optionally in seeds, said plant propagation material in a plant growth medium (optionally soil). about/at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48 A method comprising, consisting essentially of, or consisting of applying an hour or more before.
[68] The isolate of item 1, the biologically pure culture of any one of items 2-4, the complete broth culture of item 5, the cell-free filtrate of item 6, the cell extract of item 7. or about inoculating the inoculum composition of any one of items 8-43 into the plant propagation material, optionally into the seed, and said plant propagation material into the plant growth medium (optionally soil). / at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more prior to A method comprising, consisting essentially of, or consisting of.

[69] The isolate of item 1, the biologically pure culture of any one of items 2-4, the complete broth culture of item 5, the cell-free filtrate of item 6, the cell extract of item 7. , or an inoculum composition according to any one of items 8 to 43 in plant propagation material, optionally in seeds, said plant propagation material in a plant growth medium (optionally soil). about/at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 , 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36 months or more prior to .
[70] the isolate, biologically pure culture, complete broth culture, cell-free filtrate, cell extract, or inoculum composition is in/on/on the plant or plant part; Kill fungal pests, inhibit growth of one or more fungal pests, inhibit reproduction/growth of one or more fungal pests, and/or reduce the severity of one or more fungal diseases 70. A method according to any one of items 66-69, wherein the plant propagation material is introduced in an amount/concentration effective to
[71] growth and/or yield of plants wherein said isolate, biologically pure culture, complete broth culture, cell-free filtrate, cell extract, or inoculum composition is grown from said plant propagation material; 70. A method according to any one of items 66-69, wherein said plant propagation material is applied in an amount/concentration effective to improve

[72] The isolate of item 1, the biologically pure culture of any one of items 2-4, the complete broth culture of item 5, the cell-free filtrate of item 6, the cell extract of item 7. , or an inoculum composition according to any one of items 8 to 43, into the plant growth medium, optionally into the soil. .
[73] said isolate, biologically pure culture, complete broth culture, cell-free filtrate, cell extract, or inoculum composition is obtained from said plant propagation material (optionally seed); about/at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39 planted in plant growth medium , 42, 45, 48 hours or more prior to introduction into said plant growth medium.
[74] said isolate, biologically pure culture, complete broth culture, cell-free filtrate, cell extract, or inoculum composition fertilizes said plant propagation material (optionally seeds); about/at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, planting in a plant growth medium; 22, 23, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104 weeks or more 64. The method of item 63, wherein said method is introduced into said plant growth medium.

[75] said isolate, biologically pure culture, complete broth culture, cell-free filtrate, cell extract, or inoculum composition fertilizes said plant propagation material (optionally seeds); about/at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, planting in a plant growth medium; introduced into said plant growth medium 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36 months or more prior, item 63 the method of.

[76] said isolate, biologically pure culture, complete broth culture, cell-free filtrate, cell extract, or inoculum composition is obtained from said plant propagation material (optionally seed); 64. The method of item 63, wherein the method is introduced into the plant growth medium at the time of planting said plant growth medium.
[77] the isolate, biologically pure culture, complete broth culture, cell-free filtrate, cell extract, or inoculum composition is used to convert the plant propagation material, optionally seed, into the plant; 64. The method of item 63, wherein the plant is introduced into the plant growth medium after seeding the growth medium.
[78] said isolate, biologically pure culture, complete broth culture, cell-free filtrate, cell extract, or inoculum is one or more of in/on/on said plant or plant part; of fungal pests, inhibit the growth of one or more fungal pests, inhibit the reproduction/growth of one or more fungal pests, and/or reduce the severity of one or more fungal diseases. 78. The method of any one of items 72-77, wherein the method is introduced into the plant growth medium in an amount/concentration effective to cause
[79] the isolate, biologically pure culture, complete broth culture, cell-free filtrate, cell extract, or inoculum of a plant or plant part grown in the plant growth medium; 78. A method according to any one of items 72-77, wherein the amount is introduced into said plant growth medium in an amount concentration effective to enhance growth and/or yield.

[80] A method comprising, consisting essentially of or consisting of introducing into a plant growth medium, optionally into the soil, a non-natural composition according to any one of items 44-52. .
[81] An isolate of the present disclosure, optionally E. billingiae NRRL B-67766 or a related strain thereof (e.g., E. billingiae NRRL B-67766), for treating plants or plant parts. 67766 or closely related modified microbial strains derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or its related strains (e.g. P. gessardii NRRL B-67767). or closely related modified microbial strains derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or its related strains (e.g. P. libanesis NRRL B-67769 or a closely related modified microbial strain derived from P. libanesis NRRL B-67769).
[82] Isolates of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g. E. billingiae NRRL B -67766 or a closely related modified microbial strain derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., a closely related descendant of P. gessardii NRRL B-67767) or a closely related modified microbial strain derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related progeny of P. libanesis NRRL B-67769 or P. libanesis NRRL use of closely related modified microbial strains derived from B-67769.

[83] kills one or more fungal pests, inhibits growth of one or more fungal pests, inhibits reproduction/growth of one or more fungal pests, and/or one or more fungi An isolate of the present disclosure, optionally E. billingiae NRRL B-67766 or a closely related strain thereof (e.g., a close relative of E. billingiae NRRL B-67766), to reduce disease severity. related descendants or closely related modified microbial strains derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or closely related strains thereof (e.g., closely related strains of P. gessardii NRRL B-67767) descendants or closely related modified microbial strains derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or closely related strains thereof (e.g., closely related strains of P. libanesis NRRL B-67769) Use of progeny or closely related modified microbial strains derived from P. libanesis NRRL B-67769).
[84] Optionally, to improve plant growth, root area, root biomass, root length, root surface area, root volume, shoot (shoot) diameter, shoot length, and shoot length. perimeter:length ratio, shoot biomass, shoot surface area and/or shoot volume and/or yield, optionally average number of pods (pods) per plant, average pods per plant An isolate of the present disclosure, optionally E. billingiae NRRL B-67766 or a closely related strain thereof (e.g., E. billingiae), to improve (pod) weight and/or bushel/acre. A closely related descendant of billingiae NRRL B-67766 or a closely related modified microbial strain derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., P. gessardii A closely related descendant of NRRL B-67767 or a closely related modified microbial strain derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., P. libanesis use of closely related descendants of NRRL B-67769 or closely related modified microbial strains derived from P. libanesis NRRL B-67769).

[85] E. billingiae NRRL B-67766 or a closely related strain thereof (e.g., a closely related progeny of E. billingiae NRRL B-67766 or E. billingiae NRRL B-67766 or E. billingiae NRRL B-67766 for treating plants or plant parts) use of closely related modified microbial strains derived from 67766).
[86] E. billingiae NRRL B-67766 or a closely related strain thereof (e.g., a closely related descendant of E. billingiae NRRL B-67766 or The use of closely related modified microbial strains derived from E. billingiae NRRL B-67766).
[87] to kill one or more fungal pests, to inhibit the growth of one or more fungal pests, to inhibit the reproduction/growth of one or more fungal pests, and/or one E. billingiae NRRL B-67766 or a closely related strain thereof (e.g., a closely related progeny of E. billingiae NRRL B-67766 or E. billingiae NRRL B-67766) to reduce the severity of these fungal diseases. use of closely related modified microbial strains derived from 67766).
[88] Plant growth, optionally, root area, root biomass, root length, root surface area, root volume, shoot diameter, shoot length, shoot circumference: length ratio, shoot biomass, shoot surface area and/or shoot volume and/or yield, optionally average number of pods (pods) per plant, average pods per plant E. billingiae NRRL B-67766 or a closely related strain thereof (e.g., a closely related progeny of E. billingiae NRRL B-67766 or E. billingiae NRRL B-67766 to improve (pod) weight and/or bushel/acre use of closely related modified microbial strains derived from

[89] P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., a closely related progeny of P. gessardii NRRL B-67767 or P. gessardii NRRL B-67767) for treating plants or plant parts. use of closely related modified microbial strains derived from 67767).
[90] P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., a closely related descendant of P. gessardii NRRL B-67767 or The use of a closely related modified microbial strain derived from P. gessardii NRRL B-67767).
[91] to kill one or more fungal pests, to inhibit the growth of one or more fungal pests, to inhibit the reproduction/growth of one or more fungal pests, and/or one P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., a closely related progeny of P. gessardii NRRL B-67767 or a closely related strain derived from P. gessardii NRRL B-67767) to reduce the severity of these fungal diseases. use of marginal modified microbial strains).
[92] Plant growth, optionally, root area, root biomass, root length, root surface area, root volume, shoot (shoot) diameter, shoot length, shoot circumference: length ratio, shoot biomass, shoot surface area and/or shoot volume and/or yield, optionally average number of pods (pods) per plant, average pods (pods) per plant P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., a closely related progeny of P. gessardii NRRL B-67767 or P. gessardii NRRL B-67767 to improve weight and/or bushel/acre) use of closely related modified microbial strains derived from 67767).

[93] P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related progeny of P. libanesis NRRL B-67769 or P. libanesis NRRL B-67769 or P. libanesis NRRL B-67769) for treating plants or plant parts use of closely related modified microbial strains derived from 67769).
[94] P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related descendant of P. libanesis NRRL B-67769 or The use of a closely related modified microbial strain derived from P. libanesis NRRL B-67769).
[95] to kill one or more fungal pests, to inhibit the growth of one or more fungal pests, to inhibit the reproduction/growth of one or more fungal pests, and/or one P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related progeny of P. libanesis NRRL B-67769 or a closely related strain derived from P. libanesis NRRL B-67769) to reduce the severity of these fungal diseases. use of marginal modified microbial strains).
[96] Plant growth, optionally, root area, root biomass, root length, root surface area, root volume, shoot diameter, shoot length, shoot circumference: length ratio, shoot biomass, shoot surface area and/or shoot volume and/or yield, optionally average number of pods (pods) per plant, average pods (pods) per plant P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related progeny of P. libanesis NRRL B-67769 or P. libanesis NRRL B- use of closely related modified microbial strains derived from 67769).

[97] One or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g., E. billingiae NRRL B-67766), for treating plants or plant parts closely related descendants or related modified microbial strains derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or closely related strains thereof (e.g., closely related descendants of P. gessardii NRRL B-67767 or P. gessardii NRRL B-67767). gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related descendant of P. libanesis NRRL B-67769 or P. libanesis NRRL B-67769). Use of complete broth cultures containing closely related modified microbial strains derived from
[98] One or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related thereof, for treating plant growth media, optionally soil strain (e.g., a closely related descendant of E. billingiae NRRL B-67766 or a closely related modified microbial strain derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., a closely related descendant of P. gessardii NRRL B-67767 or a closely related modified microbial strain derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or a closely related strain thereof. (eg, a closely related progeny of P. libanesis NRRL B-67769 or a closely related modified microbial strain derived from P. libanesis NRRL B-67769).

[99] to kill one or more fungal pests, to inhibit the growth of one or more fungal pests, to inhibit the reproduction/growth of one or more fungal pests, and/or one One or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g. E. billingiae NRRL B-67766), for reducing the severity of the fungal disease closely related descendants or related modified microbial strains derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or closely related strains thereof (e.g., closely related descendants of P. gessardii NRRL B-67767 or P. gessardii NRRL B-67767). gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related descendant of P. libanesis NRRL B-67769 or P. libanesis NRRL B-67769). Use of complete broth cultures containing closely related modified microbial strains derived from
[100] Plant growth, optionally, root area, root biomass, root length, root surface area, root volume, shoot diameter, shoot length, shoot circumference: length ratio, shoot biomass, shoot surface area and/or shoot volume and/or yield, optionally average number of pods (pods) per plant, average pods (pods) per plant One or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g. E. billingiae), to improve weight and/or bushels/acre A closely related descendant of NRRL B-67766 or a closely related modified microbial strain derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g. P. gessardii NRRL B-67767 or closely related modified microbial strains derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or its related strains (e.g. P. libanesis NRRL) Use of complete broth cultures containing related descendants of B-67769 or related modified microbial strains derived from P. libanesis NRRL B-67769).

[101] One or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g., E. billingiae), for treating plants or plant parts A closely related descendant of NRRL B-67766 or a closely related modified microbial strain derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g. P. gessardii NRRL B-67767 or closely related modified microbial strains derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or its related strains (e.g. P. libanesis NRRL) Use of cell-free filtrates obtained from cultures containing related descendants of B-67769 or related modified microbial strains derived from P. libanesis NRRL B-67769).
[102] One or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related thereof, for treating plant growth media, optionally soil strain (e.g., a closely related descendant of E. billingiae NRRL B-67766 or a closely related modified microbial strain derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., a closely related descendant of P. gessardii NRRL B-67767 or a closely related modified microbial strain derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or a closely related strain thereof. (eg a closely related progeny of P. libanesis NRRL B-67769 or a closely related modified microbial strain derived from P. libanesis NRRL B-67769).

[103] to kill one or more fungal pests, to inhibit the growth of one or more fungal pests, to inhibit the reproduction/growth of one or more fungal pests, and/or one One or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g. E. billingiae), for reducing the severity of the above fungal diseases A closely related descendant of NRRL B-67766 or a closely related modified microbial strain derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g. P. gessardii NRRL) B-67767 or closely related modified microbial strains derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or its related strains (e.g. P. libanesis NRRL) Use of cell-free filtrates obtained from cultures containing related descendants of B-67769 or related modified microbial strains derived from P. libanesis NRRL B-67769).
[104] Plant growth, optionally, root area, root biomass, root length, root surface area, root volume, shoot diameter, shoot length, shoot circumference: length ratio, shoot biomass, shoot surface area and/or shoot volume and/or yield, optionally average number of pods (pods) per plant, average pods (pods) per plant One or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g., E. A closely related descendant of billingiae NRRL B-67766 or a closely related modified microbial strain derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., of P. gessardii NRRL B-67767). a closely related descendant or a closely related modified microbial strain derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related descendant of P. libanesis NRRL B-67769 or P. libanesis NRRL B-67769) The use of cell-free filtrates derived from cultures containing a closely related modified microbial strain derived from . libanesis NRRL B-67769).

[105] Strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g. E. billingiae NRRL B-67766), for treating plants or plant parts or closely related modified microbial strains derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or its closely related strains (e.g., P. gessardii NRRL B-67767 closely related progeny or related modified microbial strains derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or closely related strains thereof (e.g., of P. libanesis NRRL B-67769). Use of cell extracts obtained from related progeny or related modified microbial strains derived from P. libanesis NRRL B-67769).
[106] Strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g. E. billingiae NRRL B-67766), for treating plant growth media, optionally soil. 67766 or a closely related modified microbial strain derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., a closely related descendant of P. gessardii NRRL B-67767 or A closely related modified microbial strain derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related progeny of P. libanesis NRRL B-67769 or P. libanesis NRRL B). The use of cell extracts derived from closely related modified microbial strains derived from -67769).

[107] to kill one or more fungal pests, to inhibit the growth of one or more fungal pests, to inhibit the reproduction/growth of one or more fungal pests, and/or one Strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g. E. billingiae NRRL B-67766), for reducing the severity of the above fungal diseases. or closely related modified microbial strains derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or its closely related strains (e.g., P. gessardii NRRL B-67767 closely related progeny or related modified microbial strains derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or closely related strains thereof (e.g., of P. libanesis NRRL B-67769). Use of cell extracts obtained from related progeny or related modified microbial strains derived from P. libanesis NRRL B-67769).
[108] Plant growth, optionally, root area, root biomass, root length, root surface area, root volume, shoot diameter, shoot length, shoot circumference: length ratio, shoot biomass, shoot surface area and/or shoot volume and/or yield, optionally average number of pods (pods) per plant, average pods (pods) per plant A strain of the present disclosure, optionally E. billingiae NRRL B-67766 or a closely related strain thereof (e.g., a closely related progeny of E. billingiae NRRL B-67766 or A closely related modified microbial strain derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., a closely related progeny of P. gessardii NRRL B-67767 or P. gessardii NRRL B-67767). 67767), or P. libanesis NRRL B-67769 or a closely related strain thereof (e.g., a closely related progeny of P. libanesis NRRL B-67769 or a closely related strain derived from P. libanesis NRRL B-67769). Use of cell-free filtrates obtained from cultures containing marginal modified microbial strains).

[109] One or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g., E. billingiae), for treating plants or plant parts A closely related descendant of NRRL B-67766 or a closely related modified microbial strain derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g. P. gessardii NRRL B-67767 or closely related modified microbial strains derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or its related strains (e.g. P. libanesis NRRL) B-67769 or a closely related modified microbial strain derived from P. libanesis NRRL B-67769).
[110] One or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related thereof, for treating plant growth media, optionally soil strain (e.g., a closely related descendant of E. billingiae NRRL B-67766 or a closely related modified microbial strain derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g., a closely related descendant of P. gessardii NRRL B-67767 or a closely related modified microbial strain derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or a closely related strain thereof. (e.g., a closely related progeny of P. libanesis NRRL B-67769 or a closely related modified microbial strain derived from P. libanesis NRRL B-67769). .

[111] to kill one or more fungal pests, to inhibit the growth of one or more fungal pests, to inhibit the reproduction/growth of one or more fungal pests, and/or one One or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g. E. billingiae), for reducing the severity of the above fungal diseases A closely related descendant of NRRL B-67766 or a closely related modified microbial strain derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g. P. gessardii NRRL B-67767 or closely related modified microbial strains derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or its related strains (e.g. P. libanesis NRRL) B-67769 or a closely related modified microbial strain derived from P. libanesis NRRL B-67769).

[112] Plant growth, optionally, root area, root biomass, root length, root surface area, root volume, shoot diameter, shoot length, shoot circumference: length ratio, shoot biomass, shoot surface area and/or shoot volume and/or yield, optionally average number of pods (pods) per plant, average pods (pods) per plant One or more strains of the present disclosure, optionally E. billingiae NRRL B-67766 or closely related strains thereof (e.g. E. billingiae), to improve weight and/or bushels/acre A closely related descendant of NRRL B-67766 or a closely related modified microbial strain derived from E. billingiae NRRL B-67766), P. gessardii NRRL B-67767 or a closely related strain thereof (e.g. P. gessardii NRRL B-67767 or closely related modified microbial strains derived from P. gessardii NRRL B-67767), or P. libanesis NRRL B-67769 or its related strains (e.g. P. libanesis NRRL) B-67769 or a closely related modified microbial strain derived from P. libanesis NRRL B-67769).
[113] The non-natural composition according to any one of items 44 to 52, wherein the plant or plant part is a monocotyledon, the plant of item 53, the plant part of item 54, the processed product of item 55, item 56 a kit according to any one of items 57-62, a method according to any one of items 63-80, a use according to any one of items 81-104.

[114] The non-natural composition according to any one of items 44 to 52, the plant of item 53, the plant part of item 54, the processed product of item 55, item 56, wherein the plant or plant part is a dicotyledonous plant a kit according to any one of items 57-62, a method according to any one of items 63-80, a use according to any one of items 81-104.
[115] The non-natural composition according to any one of Items 44 to 52, the plant of Item 53, the plant part of Item 54, the processed product of Item 55, and the processed product of Item 56, wherein the plant or plant part is a leguminous plant. a kit according to any one of items 57-62, a method according to any one of items 63-80, a use according to any one of items 81-104.
[116] The non-natural composition according to any one of items 44 to 52, the plant of item 53, wherein the plant or plant part is a plant other than a leguminous plant, the plant or plant part is a monocotyledonous plant, Item 54 plant part, item 55 processed product, item 56 crop, kit according to any one of items 57-62, method according to any one of items 63-80, any one of items 81-104 1. Use according to 1.

[117] said plant or plant part is of the family Amaranthaceae, optionally chard, spinach, sugar beet, or quinoa; chamomile, chicory, chrysanthemum, dahlia, daisy, echinacea, goldenrod, guayule gum, lettuce, marigold, safflower, sunflower, or zinnia; of the Brassicaceae family, optionally arugula, broccoli, bok choy, Brussels sprouts, Cabbage, cauliflower, canola, collard greens, radish, garden cress, horseradish, kale, mustard greens, radishes, oilseed rape, rutabaga, turnips, wasabi, watercress, or Arabidopsis thaliana; Cucurbitaceae, any Optionally, cantaloupe, cucumber, honeydew melon, melon, pumpkin, squash (e.g. acorn, Japanese squash, summer squash), watermelon, or zucchini; of the Fabaceae family, optionally, alfalfa , kidney bean, carob, clover, guar, lentil, mesquite, pea, peanut, soybean, tamarind, tragacanth, or broad bean; of the Malvaceae family, optionally cocoa, cotton, durian, hibiscus, kenaf, of the Poaceae family, optionally bamboo, barley, maize, fonio, turf (e.g. bahiagrass, sedgegrass, bluegrass, buffalograss, centipede grass, fescue) Fescue or Zoysia), millet, oats, ornamental grasses, rice, rye, sorghum, sugar cane, triticale, or wheat; Polygonaceae, optionally buckwheat of the Rosaceae family, optionally almonds, apples, apricots, blackberries, blueberries, cherries, peaches, plums, quince, raspberries, roses, or strawberries; of the Solanaceae family, optionally typically bell peppers, chili peppers, eggplants, petunias, potatoes, tobacco, or tomatoes; Vitaceae), optionally grape, the non-natural composition according to any one of items 44 to 52, the plant of item 53, the plant part of item 54, the processed product of item 55, the product of item 56 A crop, a kit according to any one of items 57-62, a method according to any one of items 63-80, a use according to any one of items 81-104.

[118] A culture medium is inoculated with the isolate of item 1 or the biologically pure culture of any one of items 2-4, and the microbial density in the medium is less than milligrams (mg) of inoculated medium ) and/or about 1×10 ³ to about 1×10 ¹² colony forming units per milliliter (mL), optionally about/at least 1×10 ³ , 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1 x 10 ⁷ , 1 x 10 ⁸ , 1 x 10 ⁹ , 1 x 10 ¹⁰ , 1 x 10 ¹¹ or 1 x 10 ¹² colony forming units (cfu). seeds comprising, consisting essentially of, or consisting of harvesting the microorganisms from the inoculated medium, incubating at a temperature of 37°C; and under conditions suitable to form a seed treatment solution. A method of making a treatment liquid.
[119] A synthetic microorganism consortium comprising, consisting essentially of, or consisting of the isolate of item 1 and one or more additional microorganisms.
[Deposit of biological materials]

E. billingiae NRRL B-67766 and P. gessardii NRRL B-67767 are from the Agricultural Research Service Culture Collection, 1815 North University Street, Peoria, IL 61604, USA (operated by the U.S. Department of Agriculture). Depositary of the National Center for Agricultural Applications) on April 11, 2019 under the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure.

P. libanesis NRRL B-67769 has been filed in the Agricultural Research Service Culture Collection, 1815 North University Street, Peoria, Illinois 61604, USA (Depository of the National Center for Agricultural Utilization Research, operated by the U.S. Department of Agriculture) under patent proceedings. Deposited May 10, 2019 under the Budapest Treaty on the International Recognition of the Deposit of Microorganisms above.

E. billingiae NRRL B-67766, P. gessardii NRRL B-67767 and P. libanesis NRRL B-67769 comply with 37 C.F.R. Each has been deposited under 35 U.S.C. . Each deposit represents a pure culture of the deposited strain. Each deposit will be available as required by foreign patent law in the country in which this application or its progeny corresponding application was filed. It should be understood, however, that the availability of a deposit does not constitute a license to practice the subject invention in violation of any patent rights granted by government action.

The following examples are not intended to be a detailed listing of all of the different ways in which the disclosure may be implemented or all of the features that may be added to the disclosure. Those skilled in the art will appreciate that numerous modifications and additions can be made to the various embodiments without departing from the disclosure. Accordingly, the following description is intended to describe several specific embodiments of the invention and is not intended to exhaustively identify all permutations, combinations and variations thereof.

Example 1
Strain library

The National Agriculture and Food Research Organization (NARO), an independent administrative agency in Tsukuba, Japan, maintains a collection of over 20,000 bacterial strains isolated from the leaves of plants such as barley, rice, tomato and wheat. . Tsushima, Science & Technonews Tsukuba 79:18 (2006).

A total of 503 bacterial strains were randomly selected from this collection for screening for fungicidal activity against Fusarium graminearum, the pathogen of Fusarium head blight in wheat and barley.

Example 2
Primary screening of bacterial strains
15 wheat seeds (Ayahikari cultivar (cv.Ayahikari)) were sown in potting soil per plastic nursery box (15 x 6 x 10 cm) and cultivated in a greenhouse under fluorescent light for 50-60 days. and urged the ear to emerge.

Bacteria were grown in PPG liquid medium (per 1,000 mL, 200 g potato, 5 g peptone, ₅ g glucose, ₃ g _{Na2HPO4.12H2O} , 0.5 g _KH2PO4 , ₃ g NaCl ) at 25°C. Bacteria were harvested from the medium by low speed centrifugation and then resuspended in water to a concentration of OD ₆₁₀ =0.13-0.26 (approximately 10 ⁸ CFU/mL). Tween® 20 was added to the diluted bacteria at a concentration of 0.1% (v/v).

Approximately 5 mL of each bacterial cell suspension was sprayed onto approximately 10 wheat tips using an atomizer. Negative controls (negative controls) were wheat tops that had not been sprayed with bacteria. As a positive control (positive control), a 4000-fold dilution of the chemical fungicide iminooctadine tris (abesylate) was sprayed onto the tops of wheat. The sprayed plants were left in the greenhouse for 1 day.

Conidia of Fusarium graminearum strain H3 were prepared by culturing the organisms on half-strength oatmeal agar plates for 2-3 weeks under a BLB lamp (12 hour photoperiod). Conidia were harvested from the agar plates, washed once using low speed centrifugation and then suspended in water at a concentration of ¹⁰⁵ conidia per mL. Tween 20 was added to the diluted bacteria at a concentration of 0.1% (v/v). After one day of bacterial treatment of the wheat tips (spikes), 4-5 mL of this conidial suspension was sprayed per wheat nursery box. These wheats were kept in an incubation room (20°C, almost 100% humidity) for 48 hours.

After an incubation period of 48 hours, the plants were placed in the greenhouse. After 5-7 days in the greenhouse, disease severity was assessed based on the percentage of diseased spikelets in total spikelets for each wheat. A bacterial strain was scored as active if the total number of diseased spikelets in the presence of bacteria was significantly lower than untreated controls (p<0.05).

Example 3
Secondary screening of bacterial strains
Bacterial strains recorded as having activity in the above experiments were further tested. These experiments were similar to those described in Example 2 above, but included a commercial biopesticide as an additional positive control: BOTOKILLER® (Idemitsu Kosan Co., Ltd.). ,Tokyo Japan). The best performing strains exhibited fungicidal activity similar to iminooctadine tris (abesylate) and superior to the fungicidal activity of commercial biopesticides. E. billingiae NRRL B-67766, P. gessardii NRRL B-67767 and P. libanesis NRRL B-67769 are three of these top performing strains. is a stock.

Example 4
Identification and sequencing of specific top performing strains
The isolates deposited as NRRL B-67766, NRRL B-67767 and NRRL B-67769 were identified as Erwinia billingiae, Pseudomonas gessardii and Pseudomonas gessardii respectively by MALDI biotyping and 16S sequencing. - Identified as Pseudomonas libanesis. MALDI biotyping was performed using a Bruker MALDI-TOF (Matrix Assisted Laser Desorption Ionization-Time of Flight) mass spectrometer. NRRL B-67766, NRRL B-67767 and NRRL B-67769 were each applied to the target using the direct application method and the resulting protein spectra were compared with the Bruker BDAL library and Novozymes' registered microbial strains. Compared with internal library. The 16S ribosomal DNA sequence was determined by colony PCR and Sanger sequencing using degenerate primers targeting the 16S ribosomal gene sequence. The 16S rDNA sequences for E. billingiae NRRL B-67766, P. gessardii NRRL B-67767 and P. libanesis NRRL B-67769 are provided as SEQ ID NOS: 1-3.

SEQ ID NO: 1 (E. billingiae NRRL B-67766 16S rDNA sequence)
GACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGAGAGGTTAATAACCTCGTTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTCAAGTCGGATGTGAAATCCCCGGGCTCAACCCGGGAACTGCATTCGAAACTGGCAGGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTTCCCTTGAGGAGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGCTCTTGACATCCACGGAATTCGGCAGAGATGCCTTAGTGCCTTCGGGAACCGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTTGTTGCCAGCGATTCGGTCGGGAACTCAAAGGAGACTGCCGGTGATAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGAGCAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTCACAAAGTGCGTCGTAGTCCG GATCGGAGTCTGCAACTCGACTCCGTGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCT

SEQ ID NO: 2 (P. gessardii NRRL B-67767 16S rDNA sequence)
AGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGAGAGAAGCTTGCTTCTCTTGAGAGCGGCGGACGGGTGAGTAATGCCTAGGAATCTGCCTGGTAGTGGGGGATAACGTTCGGAAACGGACGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGACCTTCGGGCCTTGCGCTATCAGATGAGCCTAGGTCGGATTAGCTAGTTGGTGGGGTAATGGCTCACCAAGGCGACGATCCGTAACTGGTCTGAGAGGATGATCAGTCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGTTGTAGATTAATACTCTGCAATTTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCAAAACTGACTGACTAGAGTATGGTAGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACTAATACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCAACTAGCCGTTGGAAGCCTTGAGCTTTTAGTGGCGCAGCTAACGCATTAAGTTGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGCCTTGACATCCAATGAACTTTCCAG AGATGGATTGGTGCCTTCGGGAACATTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCCTTAGTTACCAGCACGTTATGGTGGGCACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGGCCTGGGCTACACACGTGCTACAATGGTCGGTACAGAGGGTTGCCAAGCCGCGAGGTGGAGCTAATCCCATAAAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTT

SEQ ID NO: 3 (P. libanesis NRRL B-67769 16S rDNA sequence)

Example 5
E. billingiae NRRL B-67766 protected plants against leaf blight, rust and powdery mildew
These studies were designed to test the ability of E. billingiae strain NRRL B-67766 to control fungal pathogens that cause leaf blight, rust and powdery mildew.

E. billingiae NRRL B-67766 was added to PPG liquid medium (200 g potato, 5 g peptone, ₅ g glucose, ₃ g _{Na2HPO4.12H2O} , 0.5 g per 1,000 mL). KH ₂ PO ₄ , 3 g NaCl) at 25°C. Bacteria were harvested from the medium by low speed centrifugation and resuspended in water to a concentration of OD ₆₁₀ =0.13-0.26 (approximately 10 ⁸ CFU/mL). Tween 20 was added to the diluted bacteria at a concentration of 0.1% (v/v).

Winter wheat cultivars were sown in 1 liter pots (15 seeds per pot) and grown under greenhouse conditions. Six pots were used for each experimental condition. After about one month, the seedlings were treated with a bacterial suspension having a concentration of about 10 ⁶ cfu/ml. The plants were sprayed with the bacterial suspension until the leaves were saturated (approximately 4 liters (L) per hectare (ha) rate). The commercially available fungicides prothioconazole [PROLINE® EC 250 (Bayer Crop Science, St. Louis, MO, USA) and SERENADE® (Bayer Crop Science, MO, USA). St. Louis)] was sprayed at a rate of 0.8 L/ha as a positive control (positive control) according to the manufacturer's instructions. Negative controls (negative controls) were untreated plants and plants sprayed with water.

One day after application of E. billingiae NRRL B-67766, the plants were inoculated with the pathogen.

For leaf blight, a mixture of 5 isolates of Septoria tritici (also known as Zymoseptoria tritici) was prepared as a suspension containing 2 x 10 ⁶ spores/mL solution. . Plants in each pot were sprayed with 2 mL of the suspension. Inoculated plants were kept in the dark for 1 day after inoculation to promote infection. It was then stored in a greenhouse under conditions of high humidity (>90%) at a temperature of 15°C. Early symptoms were observed after 3 weeks. Disease evaluation was performed 19 days, 26 days and 30 days after pathogen inoculation. The percentage of leaf area infested by pathogens was observed and the standard European Mediterranean Plant Protection Organization (EPPO) scale was used. E. billingiae NRRL B-67766 exhibited fungicidal activity similar to that of prothioconazole, reducing fungal disease by 40-60% compared to untreated controls or commercial biopesticides. The effect of E. billingiae NRRL B-67766 against Septoria was consistent across multiple trials.

For rust, plants were inoculated with spores of Puccinia striiformis using a sprayer. After inoculation, the plants were kept in a dark incubator for 20 hours. The plants were then stored in a greenhouse under conditions of about 75% relative humidity and 20°C. Initial symptoms were observed after 1 week. Disease evaluation was performed 7 days, 9 days and 14 days after pathogen inoculation. Overall infestation per pot and number of rust spots per pot were assessed. E. billingiae NRRL B-67766 reduced fungal disease by 50-70% compared to untreated controls or commercial biopesticides.

For powdery mildew, during powdery mildew trials, Blumeria graminis infection developed from a natural inoculum that was present in the environment when the plants were treated. E. billingiae NRRL B-67766 and P. gessardii NRRL B-67767 reduced fungal disease by approximately 30% compared to untreated controls or commercial biopesticides.

Example 6
E. billingiae NRRL B-67766 protected plants against Fusarium head blight
This study was designed to test the ability of E. billingiae NRRL B-67766 to control the fungal pathogen that causes head blight.

E. billingiae NRRL B-67766 was added to PPG liquid medium (200 g potato, 5 g peptone, ₅ g glucose, ₃ g _{Na2HPO4.12H2O} , 0.5 g per 1,000 mL). KH ₂ PO ₄ , 3 g NaCl) at 25°C. Bacteria were harvested from the medium by low speed centrifugation and then resuspended in water to a concentration of OD ₆₁₀ =0.13-0.26 (approximately 10 ⁸ CFU/mL). Tween® 20 was added to the diluted bacteria at a concentration of 0.1% (v/v).

Spring wheat was grown on permeable tented ground under natural weather conditions. Plants were grown to flowering and then E. billingiae NRRL B-67766 suspension was applied at a rate close to 200 liters/hectare (approximately 10 mL of biocontrol agent per plot) using a portable sprayer. Sprayed with GS 65 using. Twenty-four hours after moisturizing (24 h), the plants were inoculated with a mixture of spore suspensions of Fusarium culmorum and Fusarium graminearum. Negative controls (negative controls) were untreated plants and plants sprayed with water. Infestation assessment was performed by counting the incidence and severity of disease in the wheat tops at 15, 22 and 26 days after inoculation. E. billingiae strain NRRL B-67766 reduced fungal disease by approximately 40% over untreated controls.

Example 7
E. billingiae NRRL B-67766 protected plants against leaf blight
This study was designed to test the ability of E. billingiae NRRL B-67766 to control fungal pathogens that cause leaf blight.

E. billingiae NRRL B-67766 was added to PPG liquid medium (200 g potato, 5 g peptone, ₅ g glucose, ₃ g _{Na2HPO4.12H2O} , 0.5 g per 1,000 mL). KH ₂ PO ₄ , 3 g NaCl) at 25°C. Bacteria were harvested from the medium by low speed centrifugation and then resuspended in water to a concentration of OD ₆₁₀ =0.13-0.26 (approximately 10 ⁸ CFU/mL). Tween® 20 was added to the diluted bacteria at a concentration of 0.1% (v/v).

A small plot field trial was carried out under natural weather conditions with wheat plants grown in plots of 22 m ² . Moderate to severe outbreaks of Septoria pathogens occurred from natural inoculum sources in the field environment. Treated plants were sprayed with a commercial fungicide or E. billingiae NRRLB-67766 at three major plant growth stages, GS 32, GS 37-39, and GS 51-53. Negative controls (negative controls) were untreated plants and plants sprayed with water. Disease assessment was performed at multiple growth stages and multiple leaves after treatment with biocontrol agents. E. billingiae NRRL B-67766 reduced fungal disease by more than 40% compared to untreated controls.

Example 8
E. billingiae NRRL B-67766 exhibited antifungal activity against Zymoseptoria with different chemical resistance profiles
This study was designed to test the ability of E. billingiae NRRL B-67766 to control fungal pathogens with different chemical resistance profiles.

Three strains of Zymoseptoria tritici were treated with two commercial fungicides: triazolinethione (prothioconazole), which is thought to interfere with the synthesis of ergosterol, and succinate dehydrogenase inhibition. The challenge was with a suspected pyrazolecarboxamide (fluxapyroxad).

Each Z. tritici strain was grown on PDA plates in the presence of E. billingiae NRRL B-67766. Antifungal activity was measured by the appearance of zones of inhibition around E. billingiae colonies. E. billingiae NRRL B-67766 exhibited antagonism against all three strains of Z. tritici. This suggests that E. billingiae NRRL B-67766 can protect plants against fungal pests with a wide variety of chemical resistance profiles and may act through multiple mechanisms of action. .

Example 9
E. billingiae NRRL B-67766 was phytogenetically distinct from pathogenic strains of E. amylovora
A pipeline has been developed to identify unique genomic regions within a population of strains of prokaryotic species. Using this pipeline, E. billingiae NRRL B-67766 can be compared with other Erwinia strains of various species by building a high-resolution strain-level taxonomic assessment using variable sites within the core genome alignment. compared to Genome-wide plant development derived from 7906 high-quality SNPs yields clear separation of clade containing E. billingiae NRRL B-67766 from clade containing pathogenic E. amylovora strains rice field.

Example 10
Extracts of E. billingiae NRRL B-67766 culture showed activity against Zymoseptoria and Fusarium
This test is to test the ability of cell-free filtrates and cell extracts obtained from cultures of E. billingiae NRRL B-67766 to control the fungal pathogens that cause leaf blight and Fusarium head blight. planned to

E. billingiae NRRL B-67766 was cultured in four different media: Proprietary Proprietary Medium 1, Proprietary Medium 2, Potato-Glucose Broth (P6685; Sigma-Aldrich, St. Louis, Mo., USA) and PPG liquid. culture medium (200 g potato, 5 g peptone, 5 g glucose, ₃ g _{Na2HPO4.12H2O} , 0.5 g _KH2PO4 , ₃ g NaCl _per 1,000 mL) at 25°C. and cultured.

Extracts of E. billingiae NRRL B-67766 cultures were prepared by lysing the cells, centrifuging to remove cell debris, and evaporating the resulting supernatant.

Each extract was screened for activity against Zymoseptoria tritici CBS 115943 and Fusarium graminearum BREM 1982. Proprietary medium 1 extract reduced viability of Fusarium graminearum BRFM 1982 and Zymoseptoria tritici CBS 115943 by approximately 40% and 50-75%, respectively, compared to the uninoculated medium control extract. Proprietary medium 2 and PDB extract reduced viability of Zymoseptoria tritici CBS 115943 by 50-75% compared to uninoculated control extract.

Example 11
E. billingia NRRL B-67766 protected plants against fungal disease
This study was designed to test the ability of E. billingiae NRRL B-67766 to protect plants from fungal disease in field fields.

E. billingiae NRRL B-67766 was added to PPG liquid medium (200 g potato, 5 g peptone, ₅ g glucose, ₃ g _{Na2HPO4.12H2O} , 0.5 g per 1,000 mL). KH ₂ PO ₄ , 3 g NaCl) at 25°C. Bacteria were harvested from the medium by low speed centrifugation and resuspended in water to a concentration of OD ₆₁₀ =0.13-0.26 (approximately 10 ⁸ CFU/mL). Tween® 20 was added to the diluted bacteria at a concentration of 0.1% (v/v).

Winter wheat cultivars (Bergamo, Bermude, Cellude, Hereford, Informer, RGT Volupto and Torp) were sown in 7 different field fields in Europe (4 in France and 3 in Denmark). Two commercially available chemical fungicides [Prothioconazole, PROLINE® EC250, Bayer Crop Science, St. Louis, MO, USA; Mitoconazole, JUVENTUS®, BASF Crop Protection, Germany Ludwigshachen], two commercial biofungicides [SERENADE®, Bayer Crop Science, St. Louis, MO, USA; Castets, France] or E. billingiae NRRL B-67766 suspension was sprayed at three time points (BBCH31-33; BBCH35-37; BBCH39). Disease severity was assessed after treatment at each time point and again at 20, 30 and 40 days after the final treatment.

Plants treated with E. billingiae NRRL B-67766 showed reduced fungal disease severity and increased yield compared to untreated control plants and plants treated with a commercial biopesticide. rice field.

Example 12
P. gessardii NRRL B-67767 protected plants against leaf blight and powdery mildew
These studies were designed to test the ability of P. gessardii NRRL B-67767 to control fungal pathogens that cause leaf blight and powdery mildew.

E. billingiae NRRL B-67766 was added to PPG liquid medium (200 g potato, 5 g peptone, ₅ g glucose, ₃ g _{Na2HPO4.12H2O} , 0.5 g per 1,000 mL). KH ₂ PO ₄ , 3 g NaCl) at 25°C. Bacteria were harvested from the medium by low speed centrifugation and then resuspended in water to a concentration of OD ₆₁₀ =0.13-0.26 (approximately 10 ⁸ CFU/mL). Tween® 20 was added to the diluted bacteria at a concentration of 0.1% (v/v).

Winter wheat cultivars were sown in 1 liter pots (15 seeds per pot) and grown under greenhouse conditions. Six pots were used for each test condition. After about one month, the seedlings were treated with a bacterial suspension having a concentration of about 10 ⁶ cfu/ml. The plants were sprayed with the bacterial suspension until foliar saturation (at a rate of approximately 4 L/ha). The commercial fungicides prothioconazole (PROLINE® EC250, Bayer Crop Science, St. Louis, MO, USA) and SERENADE® (Bayer Crop Science, St. Louis, MO, USA) were used as positive controls ( As a positive control), it was applied at a rate of 0.8 L/ha according to the manufacturer's instructions. Negative controls (negative controls) were untreated plants and plants sprayed with water.

The plants were inoculated with the pathogen one day after spraying with the bacteria.

For leaf blight, a mixture of 5 isolates of Septoria tritici (also known as Zymoseptoria tritici) was prepared as a suspension containing 2 x 10 ⁶ spores/mL solution. . Plants in each pot were sprayed with 2 mL of the suspension. Inoculated plants were kept in the dark for 1 day after inoculation to promote infection. It was then stored in a greenhouse under conditions of high humidity (>90%) at a temperature of 15°C. After 3 weeks early symptoms were observed. Disease evaluation was performed 19 days, 26 days and 30 days after pathogen inoculation. The percentage of leaf area infested by pathogens was observed and the standard European Mediterranean Plant Protection Organization (EPPO) scale was used. P. gessardii NRRL B-67767 exhibited fungicidal activity similar to that of prothioconazole, reducing fungal lesions by 40-60% compared to untreated controls or commercial biopesticides. rice field.

For powdery mildew, during powdery mildew trials, Blumeria graminis (a powdery mildew pathogen of the grass family) infection developed from a natural inoculum that was present in the environment when the plants were treated. P. gessardii B-67767 reduced fungal disease by approximately 30% compared to untreated controls or commercial biopesticides.

Example 13
Extracts of P. gessardii NRRL B-67767 culture showed activity against Zymoseptoria and Fusarium
This test tests the ability of cell-free filtrates and cellular extracts from cultures of P. gessardii NRRL B-67767 to control the fungal pathogens that cause leaf blight and Fusarium head blight. planned to

P. gessardii NRRL B-67767 was cultured in four different media: Proprietary Proprietary Medium 1, Proprietary Medium 2, Potato-Glucose Broth (P6685; Sigma-Aldrich, Missouri, USA). St. Louis) and PPG liquid medium (200 g potato, 5 g peptone, 5 g glucose, ₃ g _{Na2HPO4.12H2O} , 0.5 g _KH2PO4 , ₃ g NaCl _per 1,000 mL) cultured at 25°C.

Extracts of P. gessardii NRRL B-67767 cultures were prepared by lysing the cells, centrifuging to remove cell debris, and evaporating the resulting supernatant.

Each extract was screened for activity against Zymoseptoria tritici (cause of rubber tree leaf blight) CBS 115943 and Fusarium graminearum (cause of rubber tree head blight) BREM 1982.

The Proprietary Medium 1 extract reduced viability of Fusarium graminearum BRFM 1982 by 30-45% compared to the uninoculated medium control extract. Proprietary medium 2 and PDB extract reduced viability of Zymoseptoria tritici CBS 115943 by 65-80% compared to uninoculated control extract.

Example 14
E. billingiae NRRL B-67766 and P. gessardii NRRL B-67767 persisted on wheat leaves
This study was designed to test the ability of E. billingiae NRRL B-67766 and P. gessardii NRRL B-67767 to colonize and persist on the leaf surface of wheat plants. was done.

As described in Example 2, wheat leaves were sprayed at approximately 10 ⁶ CFU/mL. After spraying, leaves were collected at 24 hour intervals for 5 days, and genomic DNA was extracted from leaf samples. A qPCR-based method was developed to examine the viability of E. billingiae NRRL B-67766 and P. gessardii NRRL B-67767 on leaves. Strain-specific primers were used to follow and monitor the persistence of E. billingiae NRRL B-67766 and P. gessardii NRRL B-67767 on wheat foliage.

E. billingiae NRRL B-67766 and P. gessardii NRRL B-67767 continued to persist on the leaves of wheat plants throughout the test period.

Example 15
P. libanesis NRRLB-67769 protected plants against leaf blight
These studies were designed to test the ability of P. gessardii NRRLB-67769 to control fungal pathogens that cause leaf blight and powdery mildew.

P. libanesis NRRL B-67769 was added to PPG liquid medium (200 g potato, 5 g peptone, ₅ g glucose, ₃ g _{Na2HPO4.12H2O} , 0.5 g per 1,000 mL). KH ₂ PO ₄ , 3 g NaCl) at 25°C. Bacteria were harvested from the medium by low speed centrifugation and resuspended in water to a concentration of OD ₆₁₀ =0.13-0.26 (approximately 10 ⁸ CFU/mL). Tween® 20 was added to the diluted bacteria at a concentration of 0.1% (v/v).

Winter wheat was sown in 1 liter pots (15 seeds per pot) and grown under greenhouse conditions. Six pots were used for each test condition. After about one month, the seedlings were treated with a bacterial suspension having a concentration of about 10 ⁶ cfu/ml. The plants were sprayed with the bacterial suspension until foliar saturation (at a rate of approximately 4 L/ha). The commercial fungicides prothioconazole (PROLINE® EC250, Bayer Crop Science, St. Louis, MO, USA) and SERENADE® (Bayer Crop Science, St. Louis, MO, USA) were used as positive controls (positive Control) was applied at a rate of 0.8 l/hectare according to the manufacturer's instructions. Negative controls (negative controls) were untreated plants and plants sprayed with water.

The plants were inoculated with the pathogen one day after spraying with bacteria.

A mixture of five isolates of Septoria tritici (also known as Zymoseptoria tritici) was prepared as a suspension containing 2 x ¹⁰⁶ spores/mL solution. Plants in each pot were sprayed with 2 mL of the suspension. Inoculated plants were kept in the dark for 1 day after inoculation to promote infection. The plants were then kept in a greenhouse under conditions of high humidity (>90%) at a temperature of 15°C. After 3 weeks early symptoms were observed. Disease evaluation was performed 19 days, 26 days and 30 days after pathogen inoculation. The percentage of leaf area infested by pathogens was observed and the standard European Mediterranean Plant Protection Organization (EPPO) scale was used. P. libanesis NRRL B-67769 reduced fungal disease compared to both untreated controls and commercial biopesticides.

Appendix A
Acinetobacter, Actinomycetes, Aegerita, Agrobacterium (e.g. A. radiobacter strains e.g. K1026 and K84), Akanthomyces, Alcaligenes, Alternaria, Aminobacter (e.g. A. aganoensis, A. aminovorans, A. anthyllidis, A. ciceronei, A. lissarensis, A. niigataensis), Ampelomyces (e.g. A. quisqualis strains e.g. M-10), Anabaena (e.g. A. aequalis, A. affinis, A. angstumalis angstumalis, A. angstumalis marchita, A. aphanizomendoides, A. azollae, A. Bornetiana, A. catenula, A. cedrorum, A. circinalis, A. confervoides, A. constricta, A. cyanobacterium, A. cycadeae, A. cylindrica, A. echinispora, A. felisii, A. flos-aquae flos- aquae, A. flos-aquae minor, A. flos-aquae treleasei, A. helicoidea, A. inaequalis, A. lapponica, A. laxa, A. lemmermannii, A. levanderi, A. limnetica, A. macrospora macrospora, A. macrospora robusta, A. monticulosa, A. nostoc, A. ascillarioides, A. planctonica, A. raciborski, A. scheremetievi, A. sphaerica, A. spiroides crassa, A. sprroides sprroides, A. subcylindrica, A. torulosa, A. unispora, A. variabilis, A. verrucosa, A. viguieri, A. wisconsinense, A. zierlingii), Arthrobacter, Arthrobotrys (e.g. A. aggregata, A. alaskana, A. ameropora, A. anomala, A. apscheronica, A. arthrobotryoides, A. azerbaijanica, A. bakunika, A. botryospora, A. brochopaga, A. chazarica, A. chilensis, A. cladodes, A. calvispora, A. compacta, A. conoides, A. constringens, A. cylindrospora, A. dactyloides, A. deflectans, A. dendroides, A. doliiformis, A. drechsleri , A. elegans, A. ellipsospora, A. entomopaga, A. ferox, A. foliicola, A. fruticulosa, A. globospora, A. hatospora, A. hertziana, A. indica, A. irregularis, A. javanica, A. kirghizica, A. longa, A. longiphora, A. longiramulifera, A. longispora, A. mangrovispora, A. megaspora, A. microscaphoides, A. microspora, A. multisecundaria, A. musiformis, A. nematopaga, A. nonseptata , A. oligospora, A. oudemansii, A. oviformis, A. perpasta, A. polycephala, A. pseudoclavata, A. pyriformis, A. recta, A. robusta, A. rosea, A. scaphoides, A. sclerohypha, A. shahriari, A. shizishanna, A. sinensis, A. soprunovii, A. stilbacea, A. straminicola, A. superba, A. tabrizica, A. venusta, A. vermic ola, A. yunnanensis), Aschersonia, Ascophaera, Aspergillus (e.g. A. flavus strains e.g. NRRL 21882, A. parasiticus), Aulosira (e.g. A. aenigmatica, A. africana, A. bohemensis, A. bombayensis, A. confluens, A. fertilissima, A. fertilissma var. tenius, A. fritschii, A. godoyana, A. implexa, A. laxa, A. plantonica, A. prolifica, A. pseuodoramosa, A. schauinslandii, A. striata, A terrestris, A. thermalis), Aureobacterium, Aureobasidium (e.g. A. pullulans strains e.g. DSM 14940 and DSM 14941), Azobacter, Azorhizobium (e.g. A. caulinodans, A. doebereinerae, A. oxalatiphilum), Azospirillum (e.g. A. amazonense strains such as BR 11140 (SpY2T), A. brasilense strains such as INTAAz-39, AZ39, XOH, BR 11002, BR 11005, Ab-V5 and Ab-V6, A. canadense, A. doebereinerae, A. halopraeferans, A. irakense, A. largimobile, A. lipoferum strains e.g. agilis, A. armeniacus, A. sp.AR, A. beijerinckii, A. chroococcum, A. DCU26, A. FA8, A. nigricans, A. paspali, A. salinestris, A. tropicalis, A. vinelandii), Bacillus (e.g. B. amyloliquefaciens strains e.g. D747, NRRL B-50349, TJ1000 (also known as 1BE, isolate ATCC BAA-390), FZB24, FZB42, IN937a, IT-45, TJ1000, MBI600, BS27 (NRRL B -5015), BS2084 (deposited as NRRL B-50013), 15AP4 (deposited as ATCC PTA-6507), 3AP4 (deposited as ATCC PTA-6506), LSSA01 (NRRL B-50104 ), ABP278 (deposited as NRRL B-50634), 1013 (deposited as NRRL B-50509), 918 (deposited as NRRL B-50508), 22CP1 (deposited as ATCC PTA-6508) ) and BS18 (deposited as NRRL B-50633), B. cereus strains e.g. I-1562, B. firmus strains e.g. I-1582, B. laevolacticus, B. lichenformis strains e.g. ) and BL21 (deposited as NRRL B-50134), B. macerns, B. megaterium strains such as O62UKE (deposited as NRRL B-67352), O723G4 (deposited as NRRL B-67357), O132FX (deposited as NRRL B-67521), O14VDF (deposited as NRRL B-67522), O249FV (deposited as NRRL B-67523), O33Y38 (deposited as NRRL B-67524), O62RUA (deposited as NRRL B-67524) NRRL B-67525), O62RV8 (deposited as NRRL B-67526), O62STA (deposited as NRRL B-67527), O7237E (deposited as NRRL B-67528), O72NWX (deposited as NRRL B-67528) -67529), O72T97 (deposited as NRRL B-67530), NRRL B-67533 and NRRL B-67534, B. mycoid es strains e.g. NRRL B-21664, B. pasteurii, B. pumilus strains e.g. 55078, ATCC 55079, MBI 600, NRRL B-21661, NRRL B-21665, CX-9060, GB03, GB07, QST 713, FZB24, D747 and 3BP5 (deposited as NRRL B-50510), B. thuringiensis strains e.g. ATCC 13367, GC-91, NRRL B-21619, ABTS-1857, SAN 401 I, ABG-6305, ABG-6346, AM65-52, SA-12, SB4, ABTS-351, HD-1, EG 2348, EG 7826, EG 7841, DSM 2803, NB-125 and NB-176), Beijerinckia, Beauveria (e.g. B. bassiana strains e.g. ATCC 26851, ATCC 48023, ATCC 48585, ATCC 74040, ATCC-74250, DSM 12256 and PPRI 5339) , Beijerinckia, Blastodendrion, Bosea (e.g. B. eneae, B. lathyri, B. lupini, B. massiliensis, B. minatitlanensis, B. robiniae, B. thiooxidans, B. vestrisii), Bradyrhizobium (e.g. B. arachidis, B. bete, B. canariense, B. cytisi, B. daqingense, B. denitrificans, B. diazoefficiens, B. elkanii strains such as SEMIA 501, SEMIA 587 and SEMIA 5019, B. ganzhouense, B. huanghuauhaiense, B.icense, B. ingae, B. iriomotense, B. japonicum strains e.g. 61A227, 61A228, 6 1A273, E-109NRRL B-50586 (also deposited as NRRL B-59565), NRRL B-50587 (also deposited as NRRL B-59566), NRRL B-50588 (also deposited as NRRL B-59567) ), NRRL B-50589 (also deposited as NRRL B-59568), NRRL B-50590 (also deposited as NRRL B-59569), NRRL B-50591 (also deposited as NRRL B-59570), NRRL B-50592 (also deposited as NRRL B-59571), NRRL B-50593 (also deposited as NRRL B-59572), NRRL B-50594 (also deposited as NRRL B-50493), NRRL B -50608, NRRL B-50609, NRRL B-50610, NRRL B-50611, NRRL B-50612, NRRL B-50726, NRRL B-50727, NRRL B-50728, NRRL B-50729, NRRL B-50730, SEMIA 566 , SEMIA 5079, SEMIA 5080, USDA 6, USDA 110, USDA 122, USDA 123, USDA 127, USDA 129 and USDA 532C, B. jicamae, B. lablabi, B. liaoningense, B. manausense, B. neotropicale, B. oligotrophicum, B. ottawaense, B. pachyrhizi, B. paxllaeri, B. retamae, B. rifense, B. valentinum, B. yuanmingense), Burkholderia (e.g. B. acidipaludis, B. ambifaria, B. andropogonis, B. anthina , B. arboris, B. bannensis, B. bryophila, B. caledonica, B. caribensis, B. caryophylli, B. cenocepacua, B. choica, B. cocovenenans, B. contaminans, B. denitrificans, B. diazotrop hica, B. diffusa, B. dilworthii, B. dolosa, B. eburnea, B. endofungorum, B. ferrariae, B. fungorum, B. ginsengisoli, B. gladioli, B. glathei, B. glumae, B. graminis, B. grimmiae, B. heleia, B. hospital, B. humi, B. kururiensis, B. lata, B. latens, B. mallei, B. megapolitana, B. metallica, B. mimosarum, B. multivorans, B. nodosa, B. norimbergensis, B. oklahomensis, B. phenazinium, B. phenoliruptrix, B. phymatum, B. phytofirmans, B. pickettii, B. plantarii, B. pseudomallei, B. pseudomultivorans, B. pyrrocinia, B. rhizoxinica, B. rhynchosiae, B. sabiae, B. sacchari, B. sartisoli, B. sediminicola, B. seminalis, B. silvatlantica, B. singaporensis, B. soli, B. sordidcola, B. sp. strains such as A396, B. sprentiae, B. stabilis, B. symbiotica, B. telluris, B. terrae, B. terrestris, B. terricola, B. thailandensis, B. tropica, B. tuberum, B.ubonensis, B.udeis, B.unamae, B.vandii, B.vietnamiensis, B.xenovorans, B.zhejiangensis), Brevibacillus, Burkholderia (e.g. B. sp. A396 nov. rinojensis NRRL B-50319), Calonectria, Candida (e.g. C. oleophila e.g. I-182 , C. saitoana), Candidatus (e.g. C. Burkholderia calva, C. Burkholderia crenata, C. Burkholderia hispidae, C. Burkholderia kirkii, C. Burkholderia mamillata, C. Burkholderia nigropunctata, C. Burkholderia rigidae, C. Burkholderia schumannianae, C. Burkholderia verschuerenii, C. Burkholderia virens, C. Phytoplasma allocasuarinae, C. Phytoplasma americanum, C. Phytoplasma asteris, C. Phytoplasma aurantifolia, C. Phytoplasma australiense, C. Phytoplasma balanitae, C. Phytoplasma brasiliense, C. Phytoplasma caricae, C. Phytoplasma castaneae, C. Phytoplasma cocosnigeriae, C. Phytoplasma cocostanzaniae, C. Phytoplasma convolvuli, C. Phytoplasma costaricanum, C. Phytoplasma cynodontis, C. Phytoplasma fragariae, C. Phytoplasma fraxini, C. Phytoplasma graminis, C. Phytoplasma japonicum, C. Phytoplasma luffae, C. Phytoplasma lycopersici, C. Phytoplasma malasianum, C. Phytoplasma mali, C. Phytoplasma omanense, C. Phytoplasma oryzae, C. Phytoplasma palmae, C. Phytoplasma palmicola, C. Phytoplasma phoenicium, C. Phytoplasma pini, C. Phytoplasma pruni, C. Phytoplasm ma prunorum, C. Phytoplasma pyri, C. Phytoplasma rhamni, C. Phytoplasma rubi, C. Phytoplasma solani, C. Phytoplasma spartii, C. Phytoplasma sudamericanum, C. Phytoplasma tamaricis, C. Phytoplasma trifolii, C. Phytoplasma ulmi, C. Phytoplasma vitis, C. Phytoplasma ziphi), Chromobacterium (e.g. C. subtsugae NRRL B-30655 and PRAA4-1, C. vaccinia strains e.g. NRRL B-50880, C. violaceum), Chryseomonas, Clavibacter, Clonostachys (e.g. C. rosea f. catenulata (also called Gliocladium catenulatum) strains e.g. J1446), Clostridium, Coelemomyces, Coelomycidium, Colletotrichum (e.g. C. gloeosporioides strains e.g. ATCC 52634), Comomonas, Conidiobolus, Coniothyrium (e.g. C. minitans strains e.g. CON /M/91-08), Cordyceps, Corynebacterium, Couchia, Cryphonectria (e.g. C. parasitica), Cryptococcus (e.g. C. albidus), Cryptophlebia (e.g. C. leucotreta), Culicinomyces, Cupriavidus (e.g. C. alkaliphilus) , C. basilensis, C. campinensis, C. gilardii, C. laharis, C. metallidurans, C. numazuensis, C. oxalaticus, C. pampae, C. pauculus, C. pinatubonensis, C. respiraculi, C. taiwanensis), Curtobact erium, Cydia (e.g. C. pomonella strains e.g. V03 and V22), Dactylaria (e.g. D. candida), Delftia (e.g. D. acidovorans strain e.g. RAY209), Desulforibtio, Desulfovibrio, Devosia (e.g. D. neptuniae), Dilophosphora (e.g. D. alopecuri), Engyodontium, Enterobacter, Entomophaga, Entomophthora, Erwinia (e.g. E. billingiae e.g. O63B38 (deposited as NRRL B-67766), Erynia, Escherichia (e.g. E. intermedia), Eupenicillium, Exiguobacaterium, Filariomyces, Filobasidiella, Flavobacterium (e.g. F. H492 NRRL B-50584), Frankia (e.g. F. alni), Fusarium (e.g. F. laterium, F. oxysporum, F. solani), Gibellula, Gigaspora (e.g. , G. margarita), Gliocladium (e.g. G. virens strains e.g. ATCC 52045 and GL-21), Glomus (e.g. G. aggregatum, G. brasilianum, G. clarum, G. deserticola, G. etunicatum, G. fasciculatum , G. intraradices strains (e.g. RTI-801, G. monosporum, G. mosseae), Gluconobacter, Halospirulina, Harposporium (e.g. H. anguillulae), Hesperomyces, Hirsutella (e.g. H. minnesotensis, H. rhossiliensis, H. thomsonii strains ATCC 24874), Hydrogenophage, Hymenoscyphous (e.g. H. ericae), Hymenostilbe, Hypocrella, Isaria (e.g. I. fumosorosea strain e.g. Apopka-97 (deposited as ATCC 20874)), Klebsiella (e.g. K. pneumoniae, K. oxytoca), Kluyvera, Laccaria (e.g. L. bicolor, L. laccata), Lactobacillus, Lagenidium, Lecanicillium (e.g. L. lecanii strains e.g. KV01, L. longisporum strains e.g. KV42 and KV71), Leptolegnia, Lysinibacillus (e.g. L. sphaericus strains e.g. O62Q8F (deposited as NRRL B-67350), O62S63 ( NRRL B-67351) and O62QG9 (deposited as NRRL B-67486), Lysobacter (e.g. L. antibioticus strains e.g. 13-1 and HS124, L. enzymogenes strains e.g. 3.1T8), Massospora, Meristacrum ( M. asterospermum), Mesorhizobium (e.g. M. abyssinicae, M. albiziae, M. alhagi, M. amorphae, M. australicum, M. camelthorni, M. caraganae, M. chacoense, M. ciceri, M. gobiense , M. hawassense, M. huakuii strains such as LL32, M. loti, M. mediterraneum, M. metallidurans, M. muleiense, M. opportunistum, M. plurifarium, M. qingshengii, M. robiniae, M. sangaii, M. septentrionale, M. shangrilense, M. shonense, M. silamurunense, M. tamadayense, M. tarimense, M. temperatum, M. thiogangeticum, M. tianshanense), Metarhizium (e.g. M. anis opliae (also called M. brunneum, Metarrhizium anisopliae, and green muscadine) strains e.g. ) and ICIPE 69), M. flavoviride strains e.g. , M. chloromethanicum, M. dankookense, M. dichloromethanicum, M. extorquens, M. fujisawaense, M. gnaphalii, M. goesingense, M. gossipiicola, M. gregans, M. haplocladii, M. hispanicum, M. iners, M. isbiliense, M. jeotgali, M. komagatae, M. longum, M. lusitanum, M. marchantiae, M. mesophilicum, M. nodulans, M. organophilum, M. oryzae, M. oxalidis, M. persicinum, M. phyllosphaerae , M. platani, M. podarium, M. populi, M. radiotolerans, M. rhodesianum, M. rhodinum, M. salsuginis, M. soli, M. suomiense, M. tardum, M. tarhaniae, M. thiocyanatum, M. thurigiense, M. trifolii, M. variabile, M. zatmanii), Metschnikowia (e.g. M. fructicola), Microbacterium (e.g. M. laevaniformans, M. trichothecenolyticum strains) O34A7Y (deposited as NRRL B-67602) and O34A8B (deposited as NRRL B-67822)), Microdochium (e.g. M. dimerum), Microsphaeropsis (e.g. M. ochracea P130A), Microvirga (e.g. M. aerilata, M. aerophila, M. flocculans, M. guangxiensis, M. lotononidis, M. lupini, M. subterranea, M. vignae, M. zambiensis), Monacrosporium (e.g. M. cionopagum), Mucor, Muscodor (e.g. NRRL 30547, QST 20799 and SA-13, M. roseus strains e.g. NRRL 30548), Mycoderma, Myiophagus, Myriangium, Myrothecium (e.g. M. verrucaria), Nectria, Nematoctonus (e.g. N. leiosporus), Neozygites, Nomuraea (e.g. N. rileyi strains e.g. SA86101, GU87401, SR86151, CG128 and VA9101), Nostoc (e.g. N. azollae, N. caeruleum, N. carneum, N. comminutum, N. commune, N. ellipsosporum, N. flagelliforme, N. linckia, N. longstaffi, N. microscopicum, N. muscorum, N. paludosum, N. pruniforme, N. punctifrome, N. sphaericum, N. sphaeroides, N. spongiaeforme, N. verrucosum), Ochrobactrum (e.g. O. anthropi, O. cicero, O. cytisi, O. daejeonense, O. gallinifaecis, O. grigonense, O. guangzhouense, O. haematophilum, O. i ntermedium, O. lupini, O. oryzae, O. pectoris, O. pituitosum, O. pseudointermedium, O. pseudogrignonense, O. rhizosphaerae, O. thiophenivorans, O. tritici), Oidiodendron, Paecilomyces (e.g. P. fumosoroseus strains e.g. FE991 and FE 9901, P. lilacinus strains e.g. 251, DSM 15169 and BCP2), Paenibacillus (e.g. P. alvei strains e.g. NAS6G6, P. azotofixans, P. peoriae strains e.g. NRRL B-67884 and NRRL B-67885, P. polymyxa strains e.g. ABP166 (deposited as NRRL B-50211), Pandora, Pantoea (e.g. P. agglomerans strains e.g. NRRL B-21856, P. vagans strains e.g. C9-1), Paraglomus (e.g. P. brazilianum) , Paraisaria, Pasteuria, Pasteuria (e.g. P. nishizawae strains e.g. Pn1, P. penetrans, P. ramose, P. sp. strains e.g. ATCCPTA-9643 and ATCC SD-5832, P. thornea, P. usage), Penicillium ( For example, P. albidum, P. aurantiogriseum, P. bilaiae (formally known as P. bilaii and P. bilaiji) strains e.g. , NRRL 50778, NRRL 50777, NRRL 50778, NRRL 50779, NRRL 50780, NRRL 50781, NRRL 50782, NRRL 50783, NRRL 50784, NRRL 50785, NRRL 50786, NRRL 50787, NRRL 50788, NRRL 671674 55, NRRL 67156, NRRL 67157, NRRL 67158, NRRL 67159 and RS7B-SD1, P. brevicompactum strains e.g. AgRF18, P. canescens strains e.g. ATCC 10419, P. chyrsogenum, P. citreonigrum, P. citrinum, P. digitatum, P. expansum strains e.g. ATCC 24692 and YT02, P. fellatanum strains e.g. ATCC 48694, P. frequentas, P. fuscum, P. fussiporus, P. gaestrivorus strains e.g. glaucum, P. griseofulvum, P. implicatum, P. janthinellum strains such as ATCC 10455, P. lanosocoeruleum strains such as ATCC 48919, P. lilacinum, P. minioluteum, P. montanense, P. nigricans, P. oxalicum, P. P. pinophilum, P. purpurogenum, P. radicum strains such as ATCC 201836, FRR 4717, FRR 4719 and N93/47267, P. raistrickii strains such as ATCC 10490, P. rugulosum, P. simplicissimum, P. solitum, P. variabile, P. velutinum, P. viridicatum), Phingobacterium, Phlebiopsis (e.g. P. gigantea), Photorhabdus, Phyllobacterium (e.g. P. bourgognense, P. brassicacearum, P. catacumbae, P. endophyticum, P. ifriqiyense, P. leguminum, P. loti, P. myrsinacearum, P. sophorae, P. trifolii), Pichia (e.g. P. anomala strains e.g. WRL-076 ), Pisolithus (e.g. P. tinctorius), Planktothricoides, Plectonema, Pleurodesmospora, Pochonia (e.g. P. chlamydopora), Podonectria, Polycephalomyces, Prochlorocoous (e.g. P. marinus), Prochloron (e.g. P. didemni), Prochlorothrix, Pseudogibellula, Pseudomonas ( P. agarici, P. antartica, P. aurantiaca, P. aureofaciens, P. azotifigens, P. azotoformans, P. balearica, P. blatchfordae, P. brassicacearum, P. brenneri, P. cannabina, P. cedrina, P. cepacia, P. chlororaphis strains e.g. MA 342, P. congelans, P. corrugata, P. costantinii, P. denitrificans, P. entomophila, P. fluorescens strains e.g. ATCC 27663, CL 145A and A506, P. fragii, P. fuscovaginae, P. fulva, P. gessardii e.g. O63B39 (deposited as NRRL B-67767), P. jessenii strains e.g. PS06, P. kilonensis, P. koreensis strains e.g. O82GYH (deposited as NRRL B-67883), P. libanensis such as O63B3D (deposited as NRRL B-67769), P. lili, P. lundensis, P. lutea, P. luteola, P. mandelii, P. marginalis, P. meditrranea, P. meridana, P. migulae, P. moraviensis, P. mucidolens, P. orientalis, P. oryzihabitans, P. palleroniana, P. panacis, P. parafulva, P. peli, P. per tucinogena, P. plecoglossicida, P. protogens, P. proteolytica, P. putida, P. pyrocina strains e.g. ATCC 15958, P. rhodesiae, P. sp. strains e.g. DSM 13134, P. striata, P. stutzeri, P. syringae , P. synxantha, P. taetrolens, P. thisvervalensis, P. tolaasii, P. veronii), Pseudozyma (e.g. P. flocculosa strains e.g. PF-A22 UL), Pythium (e.g. P. oligandrum strains e.g. DV 74), Rhizobium ( R. aggregatum, R. alamii, R. alkalisoli, P. alvei, P. azibense, P. borbori, R. calliandrae, R. cauense, R. cellulosilyticum, R. daejeonense, R. endolithicum, R. endophyticum, R. etli, R. fabae, R. flavum, R. fredii, R. freirei, R. galegae, R. gallicum, R. giardinii, R. grahamii, R. hainanense, R. halophytocola, R. halotolerans, R. helanshanense , R. herbae, R. huautlense, R. indigoferae, R. jaguaris, R. kunmingense, R. laguerreae, R. larrymoorei, R. leguminosarum strains e.g. ), R. lemnae, R. leucaenae, R. loessense, R. loti strains such as 95C11 and 95C14, R. lupini, R. lusitanum, R. mayense, R. , R. multi hospitium, R. naphthalenivorans, R. nepotum, R. oryzae, R. pakistanensis, R. paknamense, R. paranaense, R. petrolearium, R. phaseoli, R. phenanthrenilyticum, R. pisi, R. pongamiae, R. populi , R. pseudoryzae, R. pusense, R. qilianshanese, R. radiobacter, R. rhizogenes, R. rhizoryzae, R. rozettiformans, R. rubi, R. selenitireeducens, R. skierneiwicense, R. smilacinae, R. soli, R. sophorae, R. sophorradicis, R. sphaerophysae, R. straminoryzae, R. subbaraonis, R. sullae, R. taibaishanense, R. tarimense, R. tibeticum, R. trifolii strains e.g. RP113-7, R. tropici strains e.g. SEMIA 4080, R. tubonense, R. undicola, R. vallis, R. viciae strains e.g. P1NP3Cst, SU303 and WSM 1455, R. vignae, R. vitis, R. yanglingense, R. yantingense), Rhizoctonia, Rhizopogon (e.g. R. amylopogon, R. fulvigleba, R. luteolus, R. villosuli), Rhodococcus, Saccharopolyspora (e.g. S. spinosa), Scleroderma (e.g. S. cepa S. citrinum), Septobasidium, Serratia, Shinella (e.g. S. kummerowiae), Sinorhizoium ( S. abri, S. adhaerens, S. americanum, S. arboris, S. chiapanecum, S. fredii strains such as CCBAU114 and USDA 205, S. garamanticus, S. indiaense, S. kostiense, S. kummerowiae, S. medicae, S. meliloti strains e.g. , S. sesbaniae, S. sojae, S. terangae, S. xinjiangense), Sorosporella, Sphaerodes (e.g. S. myco
parasitica strains (e.g. IDAC 301008-01), Spodoptera (e.g. S. littoralis), Sporodiniella, Steinernema (e.g. S. carpocapsae, S. feltiae, S. kraussei strains e.g. L137), Stenotrophomonas, Streptomyces (e.g. S. NRRL B-30145, S.M1064, S. WYE 53 (deposited as ATCC 55750), S. cacaoi strains such as ATCC 19093, S. galbus strains such as NRRL 30232, S. griseoviridis strains such as K61, S. lydicus strains such as WYEC 108 (ATCC 55445 (deposited as ATCC 55660)), S. violaceusniger strains e.g. YCED-9 (deposited as ATCC 55660)), Streptosporangium, Stillbella, Swaminathania, Talaromyces (e.g. T. aculeatus, T. flavus strains e.g. V117b), Tetranacrium, Thiobacillus, Tilachlidium , Tolypocladium, Tolypothrix, Torrubiella, Torulospora, Trenomyces, Trichoderma (e.g. T. asperellum strains e.g. SKT-1, T. atroviride strains e.g. LC52 and CNCM 1-1237, T. fertile strains e.g. JM41R, T. gamsii strains e.g. ICC 080, T. hamatum strains such as ATCC 52198, T. harzianum strains such as ATCC 52445, KRL-AG2, T-22, TH-35, T-39 and ICC012, T. polysporum, T. reesi strains such as ATCC 28217 T. stromaticum, T virens strains e.g. ATCC 58678, GL-3, GL-21 and G-41, T. viridae strains e.g. ATCC 52440, ICC080 and TV1), Typhula, Ulocladium (e.g. U. oudem ansii strains e.g. HRU3), Urdinella, Variovorax, Verticillium (e.g. V. chlamydosporum, V. lecanii strains e.g. ATCC 46578), Vibrio, Xanthobacter, Xanthomonas, Xenorhadbus, Yersinia (e.g. Y. entomophaga strains e.g. MH96 (also known as O43NEW) (deposited as DSM 18238, DSM 22339, ATCC BAA-1678 and NRRL B-67598), O82KB8, O24G3R (deposited as NRRL B-67599), O24KEK (deposited as NRRL B-67600), O333A4 ( NRRL B-67601), O23ZMJ, O348UX and O33ZDX), Zoophthora

Claims (15)

Erwinia isolate (E. billingiae NRRL B-67766) deposited under Accession No. NRRL B-67766;
Pseudomonas gessardii isolate with accession number NRRL B-67767; and Pseudomonas libanesis isolate with accession number NRRL B-67769. -67769)
A microbial isolate selected from the group consisting of a biologically pure culture of E. billingiae NRRL B-67766;
a biologically pure culture of P. gessardii NRRL B-67767; and
A biologically pure culture selected from the group consisting of biologically pure cultures of P. libanesis NRRL B-67769. E. billingiae NRRL B-67766 and/or P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769 and/or any of said strains A cell-free filtrate obtained from a bacterial culture containing one or more closely related strains. E. billingiae NRRL B-67766 and/or P. gessardii NRRL B-67767 and/or P. libanesis NRRL B-67769 and/or any of said strains Cell extracts obtained from one or more closely related strains. Cell-free filtrate obtained from E. billingiae NRRL B-67766 or its relatives, E. billingiae NRRL B-67766 or its relatives, E. billingiae Cell extracts from NRRL B-67766 or its relatives, P. gessardii NRRL B-67767 or its relatives, P. gessardii NRRL B-67767 or its relatives cell extracts from P. gessardii NRRL B-67767 or related strains thereof; P. libanesis NRRL B-67769 or related strains thereof; Cell-free filtrate obtained from P. libanesis NRRL B-67769 or a related strain thereof and/or a cell extract obtained from P. libanesis NRRL B-67769 or a related strain thereof A non-natural composition, comprising: A non-natural seed composition comprising plant propagation material (optionally seeds) and a coating covering at least a portion of the outer surface of said seed, said coating being the isolate of claim 1, claim 2. the cell-free filtrate of claim 3 and/or the cell extract of claim 4. Cell-free obtained from E. billingiae NRRL B-67766 or its relatives, E. billingiae NRRL B-67766 or its relatives for treating plants or plant parts Filtrate, cell extract from E. billingiae NRRL B-67766 or related strains, P. gessardii NRRL B-67767 or related strains, P. gessardii (P. gessardii) cell-free filtrate from NRRL B-67767 or its relatives, P. gessardii cell extract from NRRL B-67767 or its relatives, P. libanesis Cell-free filtrate obtained from NRRL B-67769 or a related strain thereof, P. libanesis NRRL B-67769 or a related strain thereof, and/or P. libanesis NRRL B-67769 or the use of cell extracts obtained from closely related strains thereof. E. billingiae NRRL B-67766 or its relatives, E. billingiae, for killing, inhibiting the growth and inhibiting reproduction/reproduction of fungal plant pests ) cell-free filtrate from NRRL B-67766 or its relatives, cell extract from E. billingiae NRRL B-67766 or its relatives, P. gessardii NRRL Cell-free filtrate obtained from B-67767 or its relatives, P. gessardii NRRL B-67767 or its relatives, P. gessardii NRRL B-67767 or its relatives P. libanesis NRRL B-67769 or related strains thereof, cell-free filtrate obtained from P. libanesis NRRL B-67769 or related strains thereof, and /or use of cell extracts obtained from P. libanesis NRRL B-67769 or closely related strains thereof. E. billingiae NRRL B-67766 or relatives thereof E. billingiae NRRL B-67766 or relatives thereof for reducing fungal disease severity in plants or plant parts cell extract from E. billingiae NRRL B-67766 or a related strain thereof; P. gessardii NRRL B-67767 or a related strain thereof; cell-free filtrate from P. gessardii NRRL B-67767 or a related strain thereof; cell extract from P. gessardii NRRL B-67767 or a related strain thereof; Cell-free filtrate obtained from P. libanesis NRRL B-67769 or related strains thereof, P. libanesis NRRL B-67769 or related strains thereof, and/or P. libanesis ) Use of cell extracts obtained from NRRL B-67769 or a closely related strain thereof. E. billingiae NRRL B-67766 or relatives thereof, E. billingiae NRRL B-67766 or relatives thereof for enhancing growth and/or yield in plants or plant parts Cell-free filtrates from related strains, cell extracts from E. billingiae NRRL B-67766 or related strains, P. gessardii NRRL B-67767 or related strains thereof , cell-free filtrate obtained from P. gessardii NRRL B-67767 or a related strain thereof, cell extract obtained from P. gessardii NRRL B-67767 or a related strain thereof, P A cell-free filtrate obtained from P. libanesis NRRL B-67769 or a related strain thereof, P. libanesis NRRL B-67769 or a related strain thereof, and/or P. libanesis (P. libanesis) NRRL B-67769 or a related strain thereof. Cell-free filtrate obtained from E. billingiae NRRL B-67766 or its relatives, E. billingiae NRRL B-67766 or its relatives, E. billingiae Cell extracts from NRRL B-67766 or its relatives, P. gessardii NRRL B-67767 or its relatives, P. gessardii NRRL B-67767 or its relatives cell extracts from P. gessardii NRRL B-67767 or related strains thereof; P. libanesis NRRL B-67769 or related strains thereof; Cell-free filtrate obtained from P. libanesis NRRL B-67769 or a related strain thereof and/or a cell extract obtained from P. libanesis NRRL B-67769 or a related strain thereof into a plant growth medium. Cell-free filtrate obtained from E. billingiae NRRL B-67766 or its relatives, E. billingiae NRRL B-67766 or its relatives, E. billingiae Cell extracts from NRRL B-67766 or its relatives, P. gessardii NRRL B-67767 or its relatives, P. gessardii NRRL B-67767 or its relatives cell extracts from P. gessardii NRRL B-67767 or related strains thereof; P. libanesis NRRL B-67769 or related strains thereof; Cell-free filtrate obtained from P. libanesis NRRL B-67769 or a related strain thereof and/or a cell extract obtained from P. libanesis NRRL B-67769 or a related strain thereof to the plant propagation material, optionally to the seed. Cell-free filtrate obtained from E. billingiae NRRL B-67766 or its relatives, E. billingiae NRRL B-67766 or its relatives, E. billingiae Cell extracts from NRRL B-67766 or its relatives, P. gessardii NRRL B-67767 or its relatives, P. gessardii NRRL B-67767 or its relatives cell extracts from P. gessardii NRRL B-67767 or related strains thereof; P. libanesis NRRL B-67769 or related strains thereof; Cell-free filtrate obtained from P. libanesis NRRL B-67769 or a related strain thereof and/or a cell extract obtained from P. libanesis NRRL B-67769 or a related strain thereof to the plant. A method comprising applying the non-natural composition of claim 5 to a plant or plant part; and/or introducing the non-natural composition of claim 5 into a plant growth medium. 7. A method comprising introducing the non-natural seed composition of claim 6 into a plant growth medium.

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