US20200087266A1 - Triazine compounds and pharmaceutical use thereof - Google Patents

Triazine compounds and pharmaceutical use thereof Download PDF

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US20200087266A1
US20200087266A1 US16/396,503 US201916396503A US2020087266A1 US 20200087266 A1 US20200087266 A1 US 20200087266A1 US 201916396503 A US201916396503 A US 201916396503A US 2020087266 A1 US2020087266 A1 US 2020087266A1
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Hironobu Nagamori
Ikuo Mitani
Masaki Yamashita
Takahiro Hotta
Yuichi Nakagawa
Masatoshi Ueda
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Japan Tobacco Inc
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Definitions

  • the present invention relates to a triazine compound having a microsomal prostaglandin E2 synthase-1 (mPGES-1) inhibitory activity or a pharmaceutically acceptable salt thereof, a pharmaceutical composition containing same, pharmaceutical use thereof and the like.
  • mPGES-1 microsomal prostaglandin E2 synthase-1
  • Non-steroidal anti-inflammatory drugs are often used for the treatment of diseases accompanying inflammation, fever and pain, for example, rheumatism, osteoarthritis, headache and the like.
  • NSAIDs show an anti-inflammatory action, an antipyretic action and an analgesic action by preventing production of prostanoids by inhibiting cyclooxygenase (COX).
  • COX cyclooxygenase
  • COX includes two isoforms of COX-1 which is ubiquitously distributed and constitutively expressed, and COX-2 which expression is induced by various pro-inflammatory stimulations, for example, cytokines such as interleukin-1 ⁇ (IL-1 ⁇ ) and the like.
  • COX-1 and COX-2 are enzymes that convert arachidonic acid derived from cell membrane phospholipids to prostaglandin H2 (PGH2) which is a prostanoid precursor.
  • Specific prostanoid synthases are responsible for the conversion of PGH2 to respective prostanoids (prostaglandin E2 (PGE2), prostaglandin F2 ⁇ (PGF2 ⁇ ), prostaglandin I2 (PGI2), prostaglandin D2 (PGD2), thromboxane A2 (TXA2) etc.).
  • PGE2 prostaglandin E2
  • PGF2 ⁇ prostaglandin F2 ⁇
  • PKI2 prostaglandin I2
  • PGD2 prostaglandin D2
  • TXA2 thromboxane A2
  • PGE2 is the most commonly existing prostaglandin in living organisms, and is known to be deeply involved in inflammation, pain and fever. Therefore, suppression of PGE2 production is considered the main action mechanism of NSAIDs.
  • COX-1 or COX-2 suppresses all prostanoids production in the downstream thereof. This is considered to cause side effects of NSAIDs. Since NSAIDs that non-selectively inhibit COX also suppress production of PGE2 by COX-1 and PGE2 protectively acts on stomach mucosal injury, NSAIDs are considered to suppress secretion of gastric mucus and gastric mucosal blood flow, thereby increasing the risk of stomach perforations, bleeding and the like.
  • COX-2 selective inhibitors suppress production of PGI2 having a vasodilation action and a platelet aggregation inhibitory action in vascular endothelial cells, they do not suppress production of TXA2 which is a blood coagulation factor produced by platelet COX-1. Therefore, they are considered to disrupt the balance of the blood coagulation system to increase the risk of cardiovascular disorder.
  • Microsomal prostaglandin E2 synthase-1 (mPGES-1) is an enzyme that catalyzes the final step of PGE2 biosynthesis, and belongs to the membrane-associated proteins in eicosanoid and glutathione metabolism family (MAPEG family).
  • the human mPGES-1 gene was cloned in 1999, and indicated to be constitutively expressed in placenta, prostate, testis and mammary gland (non-patent document 1). In other organs, human mPGES-1 gene expression is induced by various pro-inflammatory stimulations, conjugated with COX-2.
  • inflammatory cytokine IL-1 ⁇ and Tumor Necrosis Factor- ⁇ induce mPGES-1 expression in synovial cell, osteoblast, endothelial cell, orbital fibroblast, gingival cell, chondrocyte, endothelial cell, myocardial cell and the like.
  • LPS Lipopolysaccharide
  • mPGES-1 inhibitor is considered to selectively suppress PGE2 production only in the topical site of inflammation or tissues where mPGES-1 is expressed, and does not suppress production of prostanoids (PGI2, PGD2, PGF2 ⁇ , TXA2 etc.) other than PGE2 (non-patent documents 2, 3). Therefore, mPGES-1 inhibitor is considered to be a medicament having an efficacy equivalent to that of NSAIDs but free of side effects of NSAIDs derived from a decreased production of prostanoids other than PGE2.
  • mPGES-1 knockout mice intraperitoneal PGE2 production amount and nociceptive response per unit time significantly decrease as compared to WT mice, in the evaluation of nociceptive response by LPS stimulation which is an acute inflammatory pain model. Therefore, mPGES-1 inhibitor is considered to be an analgesic for acute inflammatory pain (non-patent documents 3, 6).
  • mPGES-1 gene of Swedish females contains some single nucleotide polymorphisms that increase the onset risk and severity of rheumatism.
  • An increase in the mPGES-1 expression is immunohistologically confirmed in the synovium of rheumatism patients showing single nucleotide polymorphism (Reference SNP ID number: r523202821) that increases severity, as compared to patients free of mutation (non-patent document 5).
  • mPGES-1 knockout mice intraarticular infiltration of inflammatory cells, articular destruction and tumentia of the four limbs are markedly suppressed in a collagen-induced arthritis model, which is an animal model of rheumatism, as compared to WT mice (non-patent document 6). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug for rheumatism.
  • mRNA expression of mPGES-1 increases in meniscus cells of osteoarthritis patients (non-patent document 7).
  • mPGES-1 inhibitor reduces nociceptive responses in osteoarthritis model using monoiodoacetic acid, as compared to WT mice (patent document 1). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug for osteoarthritis.
  • mPGES-1 knockout mice body temperature elevation due to LPS stimulation is suppressed as compared to WT mice (non-patent document 8). Therefore, mPGES-1 inhibitor is considered to be an antipyretic drug.
  • mPGES-1 inhibitor is considered to be a therapeutic drug for Alzheimer's disease.
  • EP4 gene of multiple sclerosis patients contains some single nucleotide polymorphisms that increase the onset risk (Reference SNP ID numbers: rs9292777, rs4613763, rs1044063, r56896969).
  • macrophage present in the periventricular demyelinating lesion of multiple sclerosis patients expression of mPGES-1 protein is confirmed.
  • mPGES-1 knockout mice PGE2 production in the spinal cord of experimental autoimmune encephalomyelitis model mice, which is an animal model of multiple sclerosis, is suppressed, and progression of paralysis is suppressed, as compared to WT mice, (non-patent document 10). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug for multiple sclerosis.
  • mPGES-1 knockout mice PGE2 production in vascular endothelial cells of high-fat fed low density lipoprotein (LDL) receptor deficient mice, which is an atherosclerosis model, decreases, and atheroma formation is delayed as compared to WT mice.
  • LDL low density lipoprotein
  • mPGES-1 inhibitor is considered to be a prophylactic or therapeutic drug for arteriosclerosis.
  • Glaucoma is a disease showing a characteristic change in the optic nerve and the field of vision. Optic nerve disorder can be generally improved or suppressed by sufficiently decreasing the intraocular pressure. Glaucoma can be categorized into open angle glaucoma and closed angle glaucoma.
  • mPGES-1 gene is constitutively highly expressed in human conjunctiva (GEO accession No: GSE2513 (Gene Expression Omnibus:http://www.ncbi.nlm.nih.gov/geo/)).
  • GSE2513 Gene Expression Omnibus:http://www.ncbi.nlm.nih.gov/geo/
  • expression of mPGES-1 increases as compared to healthy individuals.
  • expression of mPGES-1 increases as compared to normal animals (GEO accession No: human GSE2378, dog GSE21879, mouse GSE3554).
  • PGE2 When PGE2 is instilled into the eyes of healthy individuals, the intraocular pressure increases, along with the expansion of blood vessels, for 2 hours after instillation (non-patent document 12).
  • PGE2 When PGE2 is administered to rabbits subconjunctivally, the intraocular pressure increases due to dilatation of ciliary body and increase in the aqueous humor production (non-patent document 13).
  • PGF2 ⁇ and PGD2 which are prostaglandins that may increase when mPGES-1 is inhibited, decrease the intraocular pressure of rabbit (non-patent document 14).
  • PGF2 ⁇ formulations increase outflow of aqueous humor and are used as therapeutic drugs for glaucoma that decrease the intraocular pressure.
  • PGI2 does not show a clear action on the intraocular pressure of rabbits. That is, the intraocular pressure is considered to decrease since decrease of PGE2 suppresses aqueous humor production by mPGES-1 inhibition, and/or since increased PGD2 and PGF2 ⁇ promote outflow of aqueous humor due to shunt. Also, PGE2 promotes expression of vascular endothelial growth factor (VEGF) from retina (non-patent document 15).
  • VEGF vascular endothelial growth factor
  • mPGES-1 inhibitor Since VEGF produced in retina transfers to the anterior ocular segment to cause angiogenesis glaucoma, which is increase of the intraocular pressure that is caused by obstruction of corner angle due to angiogenesis in iris, mPGES-1 inhibitor is considered to show an improvement or prophylactic effect on angiogenesis glaucoma as well. Furthermore, considering an anti-inflammatory action by the inhibition of PGE2 production, mPGES-1 inhibitor is applicable to patients having intraocular inflammation, who require careful administration of the existing prostaglandin formulations (latanoprost etc.). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug also effective for glaucoma having various background diseases.
  • VEGF vascular endothelial growth factor
  • PGE2 promotes expression of VEGF (non-patent document 15)
  • mPGES-1 inhibitor is considered to improve these diseases.
  • mPGES-1 increases in the skin of systemic scleroderma patients, as compared to healthy individuals. Similarly, expression of mPGES-1 increases in the skin of bleomycin induced scleroderma model mice, which is a systemic scleroderma model, as compared to the skin of normal mice. As compared to WT mice, mPGES-1 knockout mice showed a decrease in the accumulation of macrophage in the dermal lesion of bleomycin induced scleroderma model mice, and mitigation of cutaneous thickening, deposition of extracellular matrix and increase in the collagen content (non-patent document 16). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug for systemic scleroderma.
  • mPGES-1 knockout mice the polyp number and size were markedly suppressed in azoxymethane-induced colorectal cancer model mice, which are animal model of colorectal cancer, as compared to WT mice.
  • PGE2 production in large intestinal tumor tissue decreased and production amount of PGI2 that inhibits adhesion of cancer cells and PGD2 that induces cell death via peroxisome proliferator-activated receptor ⁇ (PPAR ⁇ ) increased, as compared to WT mice.
  • mPGES-1 inhibitor is considered to be an anticancer drug that suppresses the growth and metastasis of cancer including colorectal cancer.
  • inflammatory symptoms and/or pain relating to the conditions thereof, for which NSAIDs are effective for example, arthritis, gout, nephrolithiasis, urolithiasis, headache, menstrual pain, toothache, lumbago, muscular pain, periarthritis scapulohumeralis, cervical syndrome, temporomandibular disorder, and postoperative or posttraumatic inflammation and pain, and inflammation and pain after tooth extraction can be mentioned.
  • acute and chronic non-bacterial inflammation of eye can be mentioned and, for example, uveitis, allergic conjunctivitis and postoperative inflammation and ophthalmalgia in intraocular operation can be mentioned.
  • the main mechanism for the efficacy of NSAIDs is considered to be the suppression of PGE2 production, which is an inflammation promoting substance. Since mPGES-1 inhibitor also has a suppressive action on the PGE2 production, it is considered to be a therapeutic drug for these diseases.
  • the mPGES-1 inhibitor is considered to be beneficial for the prophylaxis or treatment of pain, rheumatism, osteoarthritis, fever, Alzheimer's disease, multiple sclerosis, arteriosclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer including colorectal cancer and diseases for which suppression of PGE2 production is effective.
  • the present invention aims to provide a triazine compound having an mPGES-1 inhibitory activity or a pharmaceutically acceptable salt thereof, a pharmaceutical composition containing same, and pharmaceutical use thereof and the like.
  • a target disease for example, pain, rheumatism, osteoarthritis, fever, Alzheimer's disease, multiple sclerosis, arteriosclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer including colorectal cancer and diseases for which suppression of PGE2 production is effective can be mentioned.
  • the present inventors have found a triazine compound having an mPGES-1 inhibitory activity, which is represented by the following formula [I], and completed the present invention.
  • the present invention is as follows.
  • R a1 and R a2 are each independently hydrogen or C 1-6 alkyl
  • n is 1, 2, 3 or 4, —(C n H 2n )— may be straight or branched chain, and
  • R b is
  • R b14 and R b15 optionally form a 4-, 5- or 6-membered lactam together with the nitrogen atom the nitrogen atom that R b14 is bonded to and the carbon atom that R b15 is bonded to (said lactam is optionally substituted by 1, 2 or 3 C 1-6 alkyls, and/or optionally form a fused ring with a benzene ring),
  • C 1-6 alkyl (said C 1-6 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of halogen, C 6-10 aryl and C 1-6 alkoxy),
  • R 2 is —(C n H 2n )—R b (n is 1 or 2, —(C n H 2n )— may be straight or branched chain, and R b is
  • R 5 is the formula:
  • a pharmaceutical composition comprising the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
  • An mPGES-1 inhibitor comprising the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof.
  • a therapeutic or prophylactic agent for glaucoma or ocular hypertension comprising the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof, and one or more kinds of other therapeutic agents for glaucoma in combination.
  • a method of inhibiting mPGES-1 comprising administering a pharmaceutically effective amount of the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof to a human.
  • a method of treating or preventing pain, rheumatism, fever, osteoarthritis, arteriosclerosis, Alzheimer's disease, multiple sclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer or a disease for which suppression of PGE2 production is effective comprising administering a pharmaceutically effective amount of the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof to a human.
  • the method of [18] for treating or preventing glaucoma or ocular hypertension further comprising administering a pharmaceutically effective amount of one or more kinds of other therapeutic agents for glaucoma to the human.
  • the compound of the present invention is effective as a therapeutic or prophylactic agent for pain, rheumatism, fever, osteoarthritis, arteriosclerosis, Alzheimer's disease, multiple sclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer including colorectal cancer, a disease for which suppression of PGE2 production is effective and the like.
  • FIG. 1 shows effect of a test article (compounds of Example 2-98), a reference article (Xalatan (registered trademark)) or a vehicle (methylcellulose, MC) on the intraocular pressure immediately before and after administration in Macaca fascicularis.
  • halogen is fluoro, chloro, bromo or iodo.
  • C 1-6 alkyl means straight chain or branched chain alkyl having 1 to 6 carbon atoms. Examples thereof include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, isopentyl, neopentyl, 1-ethylpropyl, hexyl, isohexyl, 1,1-dimethylbutyl, 2,2-dimethylbutyl, 3,3-dimethylbutyl, 2-ethylbutyl and the like.
  • the “C 1-8 alkyl” means straight chain or branched chain alkyl having 1 to 8 carbon atoms. Examples thereof include methyl, ethyl, propyl, isopropyl, 1,1-dimethylpropyl, 1-ethyl-propyl, 1-methyl-1-ethyl-propyl, butyl, isobutyl, sec-butyl, tert-butyl, 1-methyl-1-propyl-butyl, pentyl, isopentyl, neopentyl, 1-ethylpropyl, hexyl, isohexyl, 1,1-dimethylbutyl, 2,2-dimethylbutyl, 3,3-dimethylbutyl, 2-ethylbutyl and the like.
  • C 1-6 alkoxy means alkoxy wherein the alkyl moiety is the above-defined “C 1-6 alkyl”. Examples thereof include methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, sec-butoxy, tert-butoxy, pentyloxy, isopentyloxy, neopentyloxy, 1,2-dimethylpropyloxy, 1-ethylpropyloxy, hexyloxy, isohexyloxy, 1,2,2-trimethylpropyloxy, 1,1-dimethylbutyloxy, 2,2-dimethylbutyloxy, 3,3-dimethylbutyloxy, 2-ethylbutyloxy and the like.
  • haloC 1-4 alkyl means straight chain or branched chain alkyl having 1-4 carbon atoms, which is substituted by 1 to 9 the above-defined “halogens”. When it is substituted by plural halogens, respective halogens may be the same or different.
  • Examples thereof include 2-fluoroethyl, 2-chloroethyl, 2-bromoethyl, 3-fluoropropyl, 3-chloropropyl, 4-fluorobutyl, 4-chlorobutyl, 1,1-difluoroethyl, 1,1-difluoropropyl, 1,1-difluoro-2-methylpropyl, trifluoromethyl, 2,2,2-trifluoroethyl, 3,3,3-trifluoropropyl, 4,4,4-trifluorobutyl, pentafluoroethyl, 2,2,2-trifluoro-1-trifluoromethyl-ethyl and the like.
  • haloC 1-4 alkoxy means alkoxy wherein the alkyl moiety is the above-defined “haloC 1-4 alkyl”. Examples thereof include fluoromethoxy, chloromethoxy, bromomethoxy, 2-fluoroethoxy, 2-chloroethoxy, 2-bromoethoxy, 3-fluoropropoxy, 3-chloropropoxy, 4-fluorobutoxy, 4-chlorobutoxy, 1,1-difluoroethoxy, 2,2-difluoroethoxy, 1,1-difluoropropoxy, 2,2-difluoropropoxy, 3,3-difluoropropoxy, 1,1-difluoro-2-methylpropoxy, trifluoromethoxy, 2,2,2-trifluoroethoxy, 3,3,3-trifluoropropoxy, 4,4,4-trifluorobutoxy, pentafluoroethoxy, 2,2,2-trifluoro-1-trifluoromethyl-ethoxy and the like.
  • hydroxyC 1-6 alkyl means the above-defined “C 1-6 alkyl” substituted by 1 or 2 hydroxy. Examples thereof include hydroxymethyl, 2-hydroxyethyl, 1-hydroxy-1-methylethyl, 1,2-dihydroxyethyl, 3-hydroxypropyl, 1-hydroxy-2,2-dimethylpropyl, 4-hydroxybutyl, 1-hydroxy-2,2-dimethylbutyl, 5-hydroxypentyl, 6-hydroxyhexyl and the like.
  • C 1-6 alkyl-carbonyl means carbonyl bonded to the above-defined “C 1-6 alkyl”. Examples thereof include acetyl, propionyl, 2,2-dimethylpropionyl, butyryl, 3-methylbutyryl, 2,2-dimethylbutyryl, pentanoyl, 4-methylpentanoyl, hexanoyl and the like.
  • C 1-6 alkyl-carbonyloxy means carbonyloxy bonded to the above-defined “C 1-6 alkyl”. Examples thereof include methylcarbonyloxy, ethylcarbonyloxy, propylcarbonyloxy, isopropylcarbonyloxy, butylcarbonyloxy, isobutylcarbonyloxy, sec-butylcarbonyloxy, tert-butylcarbonyloxy, pentylcarbonyloxy, isopentylcarbonyloxy, 2-methylbutylcarbonyloxy, 1,1-dimethylpropylcarbonyloxy, neopentylcarbonyloxy, 3,3-dimethylbutylcarbonyloxy, 1-ethylpropylcarbonyloxy, hexylcarbonyloxy and the like.
  • C 3-7 cycloalkyl means 3- to 7-membered monocyclic cycloalkyl. Examples thereof include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and cycloheptyl.
  • C 6-10 aryl means 6- to 10-membered aryl. Examples thereof include phenyl, 1-naphthyl, 2-naphthyl and the like. Of these, preferred is phenyl.
  • the “5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms” means 5- or 6-membered monocyclic heteroaryl containing, besides carbon atoms, 1, 2 or 3 hetero atoms selected from a nitrogen atom, an oxygen atom and a sulfur atom.
  • Examples thereof include furyl, thienyl, pyrrolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, imidazolyl, pyrazolyl, oxadiazolyl(1,2,5-oxadiazolyl, 1,3,4-oxadiazolyl, 1,2,4-oxadiazolyl), thiadiazolyl(1,2,5-thiadiazolyl, 1,3,4-thiadiazolyl, 1,2,4-thiadiazolyl), triazolyl(1,2,3-triazolyl, 1,2,4-triazolyl), pyridyl, pyrimidinyl, pyridazinyl, pyrazinyl, 1,3,5-triazinyl and the like. Of these, preferred is pyridyl.
  • the “4-, 5- or 6-membered saturated heterocyclyl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms” means 4-, 5- or 6-membered monocyclic saturated heterocyclyl containing, besides carbon atoms, 1, 2 or 3 hetero atoms selected from a nitrogen atom, an oxygen atom and a sulfur atom.
  • the carbon atom of the heterocycle is optionally substituted by oxo.
  • the sulfur atom is optionally monooxidized or dioxidized.
  • Examples thereof include oxetanyl, azetidinyl, tetrahydrofuryl, tetrahydropyranyl, tetrahydrothienyl, tetrahydrothiopyranyl, oxazolidinyl, isoxazolidinyl, thiazolidinyl, isothiazolidinyl, imidazolidinyl, pyrazolidinyl, pyrrolidinyl, piperidyl (including piperidino), morpholinyl (including morpholino), thiomorpholinyl (including thiomorpholino), piperazinyl, 1,1-dioxidoisothiazolidinyl, 1,1-dioxidotetrahydrothienyl, 1,1-dioxidotetrahydrothiopyranyl, 1,1-dioxidothiomorpholinyl (including 1,1-dioxidothiomorpholino) and the like.
  • saturated heterocyclyl may be partially saturated.
  • examples thereof include imidazolinyl, oxazolinyl, pyrazolinyl, thiazolinyl and the like. Of these, preferred is oxetanyl.
  • C 1-6 alkylsulfanyl means sulfanyl bonded to the above-defined “C 1-6 alkyl”. Examples thereof include methylsulfanyl, ethylsulfanyl, propylsulfanyl, isopropylsulfanyl, n-butylsulfanyl, isobutylsulfanyl, sec-butylsulfanyl, tert-butylsulfanyl, pentylsulfanyl, 1,1-dimethylpropylsulfanyl, 2,2-dimethylpropylsulfanyl, hexylsulfanyl and the like.
  • C 2-6 alkynyl means straight chain or branched chain hydrocarbon having 2 to 6 carbon atoms and at least one triple bond. Examples thereof include ethynyl, 1-propynyl, 2-propynyl, 1-butynyl, 2-butynyl, 3-butynyl, 1-methyl-2-propynyl, 3,3-dimethylbutynyl (that is, 3,3-dimethylbut-1-ynyl) and the like.
  • the “—(C n H 2n )—” means straight chain or branched chain alkylene having n carbon atoms and 2n hydrogen atoms. Examples thereof include —CH 2 —, —CH 2 CH 2 —, —CH(CH 3 )—, —CH 2 CH 2 CH 2 —, —C(CH 3 ) 2 —, —CH(CH 3 )CH 2 — and the like.
  • R 2 is (10) —(C n H 2n )—R b and R b is (k) —NR b14 C(O)R b15
  • “(ii) C 1-8 alkyl (said C 1-8 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of hydroxy, haloC 1-4 alkyl, C 1-6 alkoxy and C 6-10 aryl)” for R b15 means the above-defined “C 1-8 alkyl” substituted or not substituted by the same or different, 1, 2 or 3 substituents selected from the group consisting of hydroxy, the above-defined “haloC 1-4 alkyl”, the above-defined “C 1-6 alkoxy” and the above-defined “C 6-10 aryl”, at the substitutable position(s) thereof.
  • R b include 2-ethoxy-3-methoxypropylcarbonylamino, 1-methyl-1-methoxy-2,2,2-trifluoroethylcarbonyla
  • R 2 is (10) —(C n H 2n )—R b and R b is (k) —NR b14 C(O)R b15
  • “(iv) C 3-7 cycloalkyl (said C 3-7 cycloalkyl is optionally substituted by 1, 2, 3 or 4 substituents selected from the group consisting of C 1-6 alkyl, halogen, hydroxyC 1-6 alkyl and haloC 1-4 alkyl, and/or optionally form a fused ring with a benzene ring)” for R b15 means (1) the above-defined “C 3-7 cycloalkyl” substituted by the same or different, 1, 2, 3 or 4 substituents selected from the group consisting of the above-defined “C 1-6 alkyl”, the above-defined “halogen”, the above-defined “hydroxyC 1-6 alkyl” and the above-defined “haloC 1-4 alkyl”, at the substitutable position(s) thereof, (2) unsubstituted
  • R b14 and R b15 optionally form a 4-, 5- or 6-membered lactam together with the nitrogen atom that R b14 is bonded to and the carbon atom that R b15 is bonded to” means that R b is 2-oxo-azetidin-1-yl, 2-oxo-pyrrolidin-1-yl, 2-oxo-piperidin-1-yl or the like.
  • lactam is optionally substituted by 1, 2 or 3 C 1-6 alkyls, and/or optionally form a fused ring with a benzene ring
  • lactam in addition to the above-mentioned “lactam”, (1) the same or different 1, 2 or 3 C 1-6 alkyls defined above are present at the substitutable position(s) of the lactam, (2) one benzene ring is fused at the fusible position of the lactam, and (3) one benzene ring is fused at the fusible position of the lactam substituted by C 1-6 alkyl(s).
  • R b examples include 3,4-dimethyl-2-oxo-pyrrolidin-1-yl, 1-oxo-1,3-dihydro-isoindol-2-yl, 3,3-dimethyl-2-oxo-2,3-dihydro-indol-1-yl and the like.
  • R 1 is preferably chloro, methyl, cyano or trifluoromethyl, more preferably chloro or trifluoromethyl, and further preferably chloro.
  • R 2 is preferably
  • R 3 is preferably
  • R 4 is preferably hydrogen, fluoro, chloro, or methyl, more preferably hydrogen.
  • R 5 is preferably
  • m1 is preferably 0, 1 or 2, more preferably 1 or 2.
  • one of preferable embodiments is a compound represented by the following formula [I-A]:
  • one of the preferable other embodiments is a compound represented by the following formula [I-B]:
  • one of the preferable other embodiments is a compound represented by the following formula [I-C]:
  • a pharmaceutically acceptable salt of a compound represented by the formula [I] may be any salt as long as it forms a nontoxic salt with the compound of the present invention, and examples thereof include salts with inorganic acid, salts with organic acid, salts with inorganic base, salts with organic base, salts with amino acid and the like.
  • salts with inorganic acid examples include salts with hydrochloric acid, nitric acid, sulfuric acid, phosphoric acid, hydrobromic acid and the like.
  • salts with organic acid examples include salts with oxalic acid, maleic acid, citric acid, fumaric acid, lactic acid, malic acid, succinic acid, tartaric acid, acetic acid, trifluoroacetic acid, gluconic acid, ascorbic acid, methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid and the like.
  • salts with organic acid examples include salts with adipic acid, alginic acid, 4-aminosalicylic acid, anhydromethylenecitric acid, benzoic acid, calcium edetate, camphoric acid, camphor-10-sulfonic acid, carbonic acid, edetic acid, ethane-1,2-disulfonic acid, dodecylsulfuric acid, ethanesulfonic acid, glucoheptonic acid, glucuronic acid, glucoheptonic acid, glycollyarsanilic acid, hexylresorcinic acid, hydrofluoric acid, hydroiodic acid, hydroxy-naphtoic acid, 2-hydroxy-1-ethanesulfonic acid, lactobionic acid, mandelic acid, methylsulfuric acid, methylnitric acid, methylenebis(salicylic acid), galactaric acid, naphthalene-2-sulfonic acid, 2-naphtoic acid
  • salts with inorganic base examples include sodium salt, potassium salt, calcium salt, magnesium salt, ammonium salt and the like.
  • examples of the salts with inorganic base include salts with aluminum, barium, bismuth, lithium, or zinc.
  • salts with organic base examples include salts with methylamine, diethylamine, trimethylamine, triethylamine, ethanolamine, diethanolamine, triethanolamine, ethylenediamine, tris(hydroxymethyl)methylamine, dicyclohexylamine, N,N′-dibenzylethylenediamine, guanidine, pyridine, picoline, choline, cinchonine, meglumine and the like.
  • examples of the salts with organic base include salts with arecoline, betaine, clemizole, N-methylglucamine, N-benzylphenethylamine or tris(hydroxymethyl)methylamine.
  • salts with amino acid examples include salts with lysine, arginine, aspartic acid, glutamic acid and the like.
  • salts with hydrochloric acid, sulfuric acid or p-toluenesulfonic acid.
  • Various salts can be obtained by reacting a compound represented by the formula [I] with inorganic base, organic base, inorganic acid, organic acid or amino acid according to a known method.
  • a compound represented by the formula [I] or a pharmaceutically acceptable salt thereof may be present as a solvate.
  • the “solvate” is a compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, which is coordinated with a solvent molecule, and also encompasses hydrates.
  • the solvate is preferably a pharmaceutically acceptable solvate, examples thereof include a hydrate, ethanolate, dimethyl sulfoxidate and the like of a compound represented by the formula [I] or a pharmaceutically acceptable salt thereof.
  • Specific examples include semihydrate, monohydrate, dihydrate or monoethanolate of a compound represented by the formula [I], monohydrate of sodium salt or 2/3 ethanolate of dihydrochloride of a compound represented by the formula [I], and the like.
  • the solvates can be obtained by a known method.
  • a compound represented by the formula [I] may be labeled with isotope (e.g., 2 H, 3 H, 14 C, 35 S etc.).
  • the compound of the present invention may exist as a tautomer.
  • the compound of the present invention can be a single tautomer or a mixture of individual tautomers.
  • a compound represented by the formula [I] may contain a tautomer shown below
  • Such tautomer is also encompassed in the compound represented by the formula [I].
  • the compound of the present invention may have a carbon double bond.
  • the compound of the present invention can be present as E form, Z form, or a mixture of E form and Z form.
  • the compound of the present invention may contain a stereoisomer that should be recognized as a cis/trans isomer.
  • the compound of the present invention can be present as a cis form, a trans form, or mixture of a cis form and a trans form.
  • the compound of the present invention may contain one or more asymmetric carbons.
  • the compound of the present invention may be present as a single enantiomer, a single diastereomer, a mixture of enantiomers or a mixture of diastereomers.
  • the compound of the present invention may be present as an atropisomer.
  • the compound of the present invention may be present as an individual atropisomer or a mixture of atropisomers.
  • the compound of the present invention may simultaneously contain plural structural characteristics that produce the above-mentioned isomers. Moreover, the compound of the present invention may contain the above-mentioned isomers at any ratio.
  • a diastereomeric mixture can be separated into each diastereomer by conventional methods such as chromatography, crystallization and the like.
  • each diastereomer can also be formed by using a stereochemically single starting material, or by a synthesis method using a stereoselective reaction.
  • An enantiomeric mixture can be separated into each single enantiomer by a method well known in the pertinent field.
  • enantiomeric mixture can be prepared by reacting the enantiomeric mixture with a substantially pure enantiomer that is known as a chiral auxiliary.
  • the diastereomeric mixture can be separated into each diastereomer mentioned above.
  • the diastereomer mixture can be separated into each diastereomer as mentioned above.
  • the separated diastereomer can be converted to a desired enantiomer by removing the added chiral auxiliary by cleavage.
  • a mixture of enantiomers of a compound can also be directly separated by a chromatography method using a chiral solid phase well known in the pertinent field.
  • one of the enantiomers of a compound can also be obtained by using a substantially pure optically active starting material or stereoselective synthesis (asymmetric induction) of a prochiral intermediate using a chiral auxiliary and an asymmetric catalyst.
  • the absolute steric configuration can be determined based on the X-ray crystal analysis of the resultant crystalline product or intermediate.
  • a resultant crystalline product or intermediate derivatized with a reagent having an asymmetric center with a known steric configuration may be used where necessary.
  • a substantially purified compound represented by the formula [I] or a pharmaceutically acceptable salt thereof is preferable. More preferred is a compound represented by the formula [I] or a pharmaceutically acceptable salt thereof or a solvate thereof, which is purified to have a purity of more than 80%.
  • compositions examples include oral preparations such as tablet, capsule, granule, powder, troche, syrup, emulsion, suspension and the like, and parenteral agents such as external preparation, suppository, injection, eye drop, nasal preparations, pulmonary preparation and the like.
  • the pharmaceutical composition of the present invention is produced according to a method known per se in the art of pharmaceutical preparations, by mixing etc. a compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, or a solvate thereof with a suitable amount of at least one kind of pharmaceutically acceptable carrier and the like as appropriate. While the content of the compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, or a solvate thereof in the pharmaceutical composition varies depending on the dosage form, dose and the like, it is, for example, 0.00001 to 100 wt % of the whole composition.
  • “pharmaceutically acceptable carrier” examples include various organic or inorganic carrier substances conventionally used as preparation materials, for example, excipient, disintegrant, binder, glidant, lubricant and the like for solid preparations, and solvent, solubilizing agent, suspending agent, isotonicity agent, buffering agent, soothing agent, surfactant, pH adjuster, thickening agent and the like for liquid preparations. Where necessary, moreover, additives such as preservative, antioxidant, colorant, sweetening agent and the like are used.
  • excipient examples include lactose, sucrose, D-mannitol, D-sorbitol, cornstarch, dextrin, microcrystalline cellulose, crystalline cellulose, carmellose, carmellose calcium, sodium carboxymethyl starch, low-substituted hydroxypropylcellulose, gum arabic and the like.
  • disintegrant examples include carmellose, carmellose calcium, carmellose sodium, sodium carboxymethyl starch, croscarmellose sodium, crospovidone, low-substituted hydroxypropylcellulose, hydroxypropylmethylcellulose, crystalline cellulose and the like.
  • binder examples include hydroxypropylcellulose, hydroxypropylmethylcellulose, povidone, crystalline cellulose, sucrose, dextrin, starch, gelatin, carmellose sodium, gum arabic and the like.
  • Examples of the “glidant” include light anhydrous silicic acid, magnesium stearate and the like.
  • lubricant examples include magnesium stearate, calcium stearate, talc and the like.
  • solvent examples include purified water, ethanol, propylene glycol, macrogol, sesame oil, corn oil, olive oil and the like.
  • Examples of the “solubilizing agent” include propylene glycol, D-mannitol, benzyl benzoate, ethanol, triethanolamine, sodium carbonate, sodium citrate and the like.
  • suspending agent examples include benzalkonium chloride, carmellose, hydroxypropylcellulose, propylene glycol, povidone, methylcellulose, glycerol monostearate and the like.
  • isotonicity agent examples include glucose, D-sorbitol, sodium chloride, D-mannitol and the like.
  • buffering agent examples include sodium hydrogenphosphate, sodium acetate, sodium carbonate, sodium citrate and the like.
  • Examples of the “soothing agent” include benzyl alcohol and the like.
  • surfactant examples include polyoxyethylene hydrogenated castor oil, polyethylene glycol monostearate, polyoxyethylene sorbitan fatty acid ester, alkyldiaminoethylglycine, alkylbenzenesulfonate, benzethonium chloride and the like.
  • pH adjuster examples include hydrochloric acid, sulfuric acid, phosphoric acid, citric acid, acetic acid, sodium hydrogen carbonate, sodium carbonate, potassium hydroxide, sodium hydroxide, monoethanolamine, triethanolamine and the like.
  • thickening agent examples include polyvinyl alcohol, carboxyvinyl polymer, methylcellulose, hydroxyethylcellulose, polyethylene glycol, dextran and the like.
  • preservative examples include ethyl parahydroxybenzoate, chlorobutanol, benzyl alcohol, sodium dehydroacetate, sorbic acid and the like.
  • antioxidant examples include sodium sulfite, ascorbic acid and the like.
  • colorant examples include food colors (e.g., Food Color Red No. 2 or 3, Food Color Yellow No. 4 or 5 etc.), ⁇ -carotene and the like.
  • sweetening agent examples include saccharin sodium, dipotassium glycyrrhizinate, aspartame and the like.
  • the pharmaceutical composition of the present invention can be administered orally or parenterally (e.g., topical, rectal, intravenous administration etc.) to human as well as mammals other than human (e.g., hamster, guinea pig, cat, dog, swine, bovine, horse, sheep, monkey etc.).
  • the dose varies depending on the subject of administration, disease, symptom, dosage form, administration route and the like.
  • the daily dose for oral administration to an adult patient is generally within the range of about 0.1 ⁇ g to 10 g, based on the compound of the present invention as the active ingredient. This amount can be administered in one to several portions.
  • the above-mentioned compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, or a solvate thereof can be used in combination with one or a plurality of other medicaments (hereinafter to be also referred to as a concomitant drug) according to a method generally employed in the medical field (hereinafter to be referred to as combined use).
  • the administration period of the above-mentioned compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, and a concomitant drug is not limited, and they may be administered to an administration subject as combination preparation, or the both preparations may be administered simultaneously or at given intervals as individual preparations.
  • the pharmaceutical composition of the present invention and a concomitant drug may be used in the form of a kit.
  • the dose of the concomitant drug is similar to the clinically-employed dose and can be appropriately selected according to the subject of administration, disease, symptom, dosage form, administration route, administration time, combination and the like.
  • the administration form of the concomitant drug is not particularly limited, and it is only required that the compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, or a solvate thereof is combined with a concomitant drug.
  • concomitant drug examples include therapeutic agents for glaucoma such as prostaglandin preparation, ⁇ blocker, ⁇ receptor agonist, sympathetic nerve stimulation agent, a blocker, carbonic anhydrase inhibitor, anticholinesterase agent, Rho kinase inhibitor and the like.
  • prostaglandin preparation examples include isopropyl unoprostone, latanoprost, travoprost, tafluprost, bimatoprost and the like.
  • ⁇ blocker examples include timolol maleate, Befunolol hydrochloride, carteolol hydrochloride, betaxolol hydrochloride, nipradilol, levobunolol hydrochloride and the like.
  • Examples of the ⁇ receptor agonist include brimonidine tartrate and the like.
  • Examples of the sympathetic nerve stimulation agent include dipivefrin hydrochloride, pilocarpine hydrochloride and the like.
  • Examples of the a blocker include bunazosin hydrochloride and the like.
  • carbonic anhydrase inhibitor examples include dorzolamide hydrochloride, brinzolamide and the like.
  • anticholinesterase agent examples include distigmine bromide and the like.
  • Rho kinase inhibitor examples include ripasudil hydrochloride hydrate and the like.
  • An example of the specific combination of medicaments is a combination of one medicament selected from latanoprost, travoprost, tafluprost, timolol maleate, dorzolamide hydrochloride and brinzolamide, and the above-mentioned compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, or a solvate thereof.
  • the steps may be modified for efficient production of the compound, such as introduction of a protecting group into a functional group with deprotection in a subsequent step, changing the order of Production Methods and steps, appropriate use of reagents other than the exemplified reagents to promote progress of the reactions, and the like.
  • the treatment after reaction in each step may be conventional ones, where isolation and purification can be performed as necessary according to a method appropriately selected from conventional methods such as crystallization, recrystallization, distillation, partitioning, silica gel chromatography, preparative HPLC and the like, or a combination of those methods.
  • the next step may be conducted without isolation and purification.
  • An intermediate capable of forming a salt may also be obtained as a salt, or used as a salt for reactions.
  • Examples of such salt include hydrochloride of an intermediate having an amino group.
  • Hal 1 is chloro or bromo
  • Compound [3] can be obtained by the Suzuki coupling reaction of compound [1] and compound [2].
  • compound [3] can be obtained by reacting compound [1] with compound [2] under heating in a solvent in the presence of a base and a palladium catalyst.
  • a ligand may be added.
  • Not less than 1.5 equivalents of compound [1] are preferably used relative to compound [2] to prevent the Suzuki coupling reaction from progressing twice.
  • Examples of the palladium catalyst to be used for the reaction include palladium acetate, tetrakistriphenylphosphinepalladium, bis(triphenylphosphine)palladium dichloride, (bis(diphenylphosphino)ferrocene)palladium dichloride-methylene chloride complex and the like.
  • Examples of the base to be used for the reaction include inorganic bases such as alkali metal salts (e.g., potassium phosphate, sodium carbonate, sodium hydrogen carbonate, potassium carbonate, potassium acetate, sodium acetate, cesium fluoride etc.) and the like, and organic bases such as triethylamine and the like.
  • inorganic bases such as alkali metal salts (e.g., potassium phosphate, sodium carbonate, sodium hydrogen carbonate, potassium carbonate, potassium acetate, sodium acetate, cesium fluoride etc.) and the like
  • organic bases such as triethylamine and the like.
  • Examples of the ligand to be used for the reaction include organic phosphine ligands (e.g., triphenylphosphine, tricyclohexylphosphine, 2,2′-bis(diphenylphosphino)-1,1′-binaphthalene, 2-dicyclohexylphosphino-2′,6′-dimethoxybiphenyl etc.) and the like.
  • organic phosphine ligands e.g., triphenylphosphine, tricyclohexylphosphine, 2,2′-bis(diphenylphosphino)-1,1′-binaphthalene, 2-dicyclohexylphosphino-2′,6′-dimethoxybiphenyl etc.
  • solvent to be used for the reaction examples include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like; hydrocarbon solvents such as toluene, xylene, hexane and the like; polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile and the like; a mixed solvent thereof, and a mixed solvent thereof with water.
  • ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like
  • alcohol solvents such as methanol, ethanol, 1-propanol
  • Compound [1] may be a commercially available product such as 2,4-dichloro-6-methoxy-1,3,5-triazine, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Compound [5] can be obtained by the Suzuki coupling reaction of compound [3] and compound [4].
  • compound [5] can be obtained by reacting compound [3] with compound [4] under heating in a solvent in the presence of a base and a palladium catalyst. Where necessary, a ligand may be added.
  • Examples of the palladium catalyst to be used for the reaction include palladium acetate, tetrakistriphenylphosphinepalladium, bis(triphenylphosphine)palladium dichloride, (bis(diphenylphosphino)ferrocene)palladium dichloride-methylene chloride complex and the like.
  • Examples of the base to be used for the reaction include inorganic bases such as alkali metal salts (e.g., potassium phosphate, sodium carbonate, sodium hydrogen carbonate, potassium carbonate, potassium acetate, sodium acetate, cesium fluoride etc.) and the like, and organic bases such as triethylamine and the like.
  • inorganic bases such as alkali metal salts (e.g., potassium phosphate, sodium carbonate, sodium hydrogen carbonate, potassium carbonate, potassium acetate, sodium acetate, cesium fluoride etc.) and the like
  • organic bases such as triethylamine and the like.
  • Examples of the ligand to be used for the reaction include organic phosphine ligands such as triphenylphosphine, tricyclohexylphosphine, 2,2′-bis(diphenylphosphino)-1,1′-binaphthalene, 2-dicyclohexylphosphino-2′,6′-dimethoxybiphenyl and the like, and the like.
  • organic phosphine ligands such as triphenylphosphine, tricyclohexylphosphine, 2,2′-bis(diphenylphosphino)-1,1′-binaphthalene, 2-dicyclohexylphosphino-2′,6′-dimethoxybiphenyl and the like, and the like, and the like.
  • solvent to be used for the reaction examples include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like; hydrocarbon solvents such as toluene, xylene, hexane and the like; polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile and the like; a mixed solvent thereof, and a mixed solvent thereof with water.
  • ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like
  • alcohol solvents such as methanol, ethanol, 1-propanol
  • Compound [I] can be obtained by converting the alkoxy of compound [5] to hydroxy by hydrolysis.
  • R 6 is C 1-6 alkyl
  • compound [I] can be obtained by reacting compound [5] in a solvent in the presence of a base at room temperature to under heating, and neutralizing the obtained solution.
  • Examples of the base to be used for the reaction include lithium hydroxide, sodium hydroxide, potassium hydroxide, sodium methoxide and the like.
  • the solvent to be used for the reaction examples include a mixed solvent of water and alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like; and a mixed solvent thereof with ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like.
  • alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like
  • ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like.
  • Compound [2] can be obtained by, for example, Production Method 1-2.
  • L 1 is a leaving group such as bromo, iodo, trifluoromethanesulfonyloxy and the like
  • X, R 5 and m1 are as defined in the aforementioned formula [I]
  • Z is as defined in the aforementioned Production Method 1-1.
  • Compound [2] can be obtained by borating compound [6].
  • compound [2] can be obtained by reacting compound [6] with a boron reagent under heating in the presence of a base and a palladium catalyst. Where necessary, a ligand may be added
  • Examples of the boron reagent to be used for the reaction include 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi-1,3,2-dioxaborolane, 5,5,5′,5′-tetramethyl-2,2′-bi-1,3,2-dioxaborinane, tetrahydroxydiboron, 4,4,5,5-tetramethyl-1,3,2-dioxaborolane and the like.
  • Examples of the palladium catalyst to be used for the reaction include palladium acetate, tetrakistriphenylphosphinepalladium, bis(triphenylphosphine)palladium dichloride, (bis(diphenylphosphino)ferrocene)palladium dichloride-methylene chloride complex and the like.
  • Examples of the base to be used for the reaction include inorganic bases such as alkali metal salts (e.g., potassium phosphate, sodium carbonate, sodium hydrogen carbonate, potassium carbonate, potassium acetate, sodium acetate, cesium fluoride etc.) and the like, and organic bases such as triethylamine and the like.
  • inorganic bases such as alkali metal salts (e.g., potassium phosphate, sodium carbonate, sodium hydrogen carbonate, potassium carbonate, potassium acetate, sodium acetate, cesium fluoride etc.) and the like
  • organic bases such as triethylamine and the like.
  • Examples of the ligand to be used for the reaction include organic phosphorus ligands (e.g., triphenylphosphine, tricyclohexylphosphine, 2,2′-bis(diphenylphosphino)-1,1′-binaphthalene, 2-dicyclohexylphosphino-2′,6′-dimethoxybiphenyl etc.) and the like.
  • organic phosphorus ligands e.g., triphenylphosphine, tricyclohexylphosphine, 2,2′-bis(diphenylphosphino)-1,1′-binaphthalene, 2-dicyclohexylphosphino-2′,6′-dimethoxybiphenyl etc.
  • solvent to be used for the reaction examples include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like; hydrocarbon solvents such as toluene, xylene, hexane and the like; polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile and the like; a mixed solvent thereof, and a mixed solvent thereof with water.
  • ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like
  • alcohol solvents such as methanol, ethanol, 1-propanol
  • Compound [2] can also be obtained by adding an organic metal reagent to compound [6] in a solvent at ⁇ 78° C. to room temperature, and reacting the product with a boron compound at ⁇ 78° C. to room temperature.
  • organic metal reagent to be used for the reaction examples include n-butyllithium, tert-butyllithium, isopropylmagnesium chloride and the like.
  • Examples of the boron reagent to be used for the reaction include trimethyl borate, triisopropyl borate, 2-isopropoxy-4,4,5,5-tetramethyl-1,3,2-dioxaborolane and the like.
  • solvent to be used for the reaction examples include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, xylene, hexane and the like, and a mixed solvent thereof.
  • ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like
  • hydrocarbon solvents such as toluene, xylene, hexane and the like, and a mixed solvent thereof.
  • compound [6] may be a commercially available product such as those shown below, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • compound [2] may be a commercially available product such as those shown below, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Compound [4] can be obtained by, for example, Production Method 1-3.
  • R 1 , R 2 , R 3 and R 4 are as defined in the aforementioned formula [I]
  • L 1 is as defined in the aforementioned Production Method 1-2
  • Z is as defined in the aforementioned Production Method 1-1.
  • Compound [4] is compound [8a] or [8b].
  • Compound [8a] or [8b] i.e., compound [4] can be obtained by borating compound [7a] or [7b] in the same manner as in Production Method 1-2, Step 1-2.
  • Compounds [7a] and [7b] may be commercially available products such as 2-bromo-4-methylbenzonitrile and 2-bromo-3-methylphenol, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • compound [4] may be a commercially available product such as those shown below, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • R 1 , R 4 , R b15 and n are as defined in the aforementioned formula [I], can be obtained by appropriately converting the substituent of ring Cy.
  • R 5 , R 6 and m1 are as defined in the aforementioned formula [I];
  • Compound [10] can be obtained by converting the ester of compound [9] to carboxy by hydrolysis.
  • compound [10] can be obtained by reacting compound [9] in a solvent in the presence of a base at room temperature to under heating, and neutralizing the obtained solution.
  • Examples of the base to be used for the reaction include lithium hydroxide, sodium hydroxide, potassium hydroxide, sodium methoxide and the like.
  • the solvent to be used for the reaction examples include a mixed solvent of water and alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like; and a mixed solvent thereof with ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like.
  • alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like
  • ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like.
  • Compound [9] may be a commercially available product such as those shown below, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Compound [11] can be obtained by converting the carboxy of compound [10] to hydroxy by reduction.
  • compound [11] can be obtained by reacting compound [10] with a reducing agent in a solvent under ice-cooling to room temperature.
  • Examples of the reducing agent to be used for the reaction include lithium aluminum hydride, diisobutylaluminum hydride, sodium bis(2-methoxyethoxy)aluminum hydride, borane-tetrahydrofuran complex and the like.
  • solvent to be used for the reaction examples include tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether, toluene, xylene, hexane and the like and a mixed solvent thereof.
  • Compound [12] can be obtained by protecting the hydroxy group of compound [11].
  • the protection reaction can be performed by a known method according to the protecting group to be employed.
  • compound [12] when P v is methoxymethyl, compound [12] can be obtained by reacting compound [11] with chloromethyl methyl ether in a solvent such as tetrahydrofuran, 1,2-dimethoxyethane, cyclopentyl methyl ether, N,N-dimethylformamide and the like in the presence of a base such as sodium hydride and the like from ice-cooling to room temperature.
  • a solvent such as tetrahydrofuran, 1,2-dimethoxyethane, cyclopentyl methyl ether, N,N-dimethylformamide and the like
  • a base such as sodium hydride and the like from ice-cooling to room temperature.
  • Compound [13] can be obtained by borating compound [12] in the same manner as in Production Method 1-2, Step 1-2.
  • Compound [14] can be obtained by the Suzuki coupling reaction of compound [3] and compound [13] in the same manner as in Production Method 1-1, Step 1-1-2.
  • Compound [15] can be obtained by removing P v of compound [14] by hydroxy-deprotection by a conventional method.
  • the deprotection reaction can be performed by a known method according to the protecting group to be employed.
  • a treatment with an acid such as hydrochloric acid, trifluoroacetic acid, methanesulfonic acid and the like only needs to be performed in a single or mixed solvent of chloroform, 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether, ethyl acetate, ethanol, methanol, water and the like.
  • R 1 and R 4 are as defined in the aforementioned formula [I]
  • Z is as defined in the aforementioned Production Method 1-1
  • t is as defined in the aforementioned Production Method 2-1, in the same manner as in Production Method 1-1, Step 1-1-2.
  • Compound [16] can be obtained by converting the hydroxy of compound [15] to the leaving group L 2 .
  • L 2 is methanesulfonyloxy
  • compound [16] can be obtained by reacting compound [15] with methanesulfonyl chloride in a solvent in the presence of a base at room temperature.
  • L 2 is bromo
  • compound [16] can be obtained by reacting compound [15] with carbon tetrabromide in a solvent in the presence of triphenylphosphine from ice-cooling to room temperature.
  • Examples of the base to be used for the reaction include triethylamine, pyridine and the like.
  • solvent to be used for the reaction examples include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like; halogenated solvents such as dichloromethane, chloroform and the like; and polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile and the like.
  • ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like
  • hydrocarbon solvents such as toluene, hexane, xylene and the like
  • halogenated solvents such as dichlor
  • p-Toluenesulfonyl chloride and benzenesulfonyl chloride can be used instead of the above-mentioned methanesulfonyl chloride.
  • Compound [18] can be obtained by reacting compound [16] in a solvent in the presence of a base at room temperature to under heating compound [17].
  • Examples of the protecting group P w include tert-butoxycarbonyl.
  • Examples of the base to be used for the reaction include inorganic bases such as alkali metal salts (e.g., cesium carbonate, potassium phosphate, sodium carbonate, potassium carbonate etc.) and the like.
  • alkali metal salts e.g., cesium carbonate, potassium phosphate, sodium carbonate, potassium carbonate etc.
  • solvent to be used for the reaction examples include polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile and the like.
  • Compound [19] can be obtained by removing P w of compound [18] by amine-deprotection by a conventional method.
  • the deprotection reaction can be performed by a known method according to the protecting group to be employed.
  • P w is tert-butoxycarbonyl
  • a treatment with an acid such as hydrochloric acid, trifluoroacetic acid, methanesulfonic acid and the like only needs to be performed in a solvent.
  • solvent to be used for the reaction examples include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like; halogenated solvents such as dichloromethane, chloroform and the like; ester solvents such as ethyl acetate and the like; and alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like.
  • ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like
  • hydrocarbon solvents such as toluene, hexane, xylene and the like
  • halogenated solvents such as dichlor
  • Compound [21] can be obtained by a conventional amide bond forming reaction, for example, by reacting compound [19] with compound [20] in a solvent in the presence of a condensing agent and an additive. A base may be added as necessary.
  • Examples of the condensing agent to be used for the reaction include dicyclohexylcarbodiimide (DCC), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (WSC.HCl), diisopropylcarbodiimide, 1,1′-carbonyldiimidazole (CDI), O-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HATU), (benzotriazol-1-yloxy)tris(pyrrolidino)phosphonium hexafluorophosphate (PyBOP), diphenylphosphoryl azide and the like.
  • DCC dicyclohexylcarbodiimide
  • WSC.HCl 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride
  • CDI 1,1′-
  • additive to be used for the reaction examples include 1-hydroxybenzotriazole (HOBt), 1-hydroxy-7-azabenzotriazole (HOAt), N-hydroxysuccinimide (HOSu), 4-dimethylaminopyridine and the like.
  • Examples of the base to be used for the reaction include organic bases such as pyridine, triethylamine and the like.
  • solvent to be used for the reaction examples include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like; halogenated solvents such as dichloromethane, chloroform and the like; and polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile, pyridine and the like. These may be used singly or as a mixture of two or more kinds thereof.
  • ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like
  • hydrocarbon solvents such as toluene, hex
  • Compound [20] may be a commercially available product such as cyclopentanecarboxylic acid and 1-(trifluoromethyl)cyclopropane-1-carboxylic acid, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Compound [21] can be indicated as compound [22].
  • Compound [I-a1] can be obtained by converting the alkoxy of compound [22] to hydroxy by hydrolysis in the same manner as in Production Method 1-1, Step 1-1-3.
  • Rx is C 1-6 alkyl or chloro
  • Compound [10a] can be obtained by halogenating compound [24].
  • compound [10a] can be obtained by reacting compound [24] with N-iodosuccinimide in an acid at room temperature.
  • Examples of the acid to be used for the reaction include concentrated sulfuric acid and the like.
  • Compound [24] may be a commercially available product such as 4-chlorophenylacetic acid, 3-(4-chlorophenyl)propionic acid, 4-(4-chlorophenyl)butanoic acid, 4-methylphenylacetic acid and 2-(4-methylphenyl)propionic acid, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Compound [26] can be obtained by borating compound [25] in the same manner as in Production Method 1-2, Step 1-2.
  • Compound [25] may be a commercially available product such as 1-(3-bromo-4-chlorophenyl)propan-1-one and 1-(3-bromo-4-chlorophenyl)butan-1-one, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Compound [27] can be obtained by the Suzuki coupling reaction of compound [3] and compound [26] in the same manner as in Production Method 1-1, Step 1-1-2.
  • Compound [28] can be obtained by converting the carboxy of compound [27] to hydroxy by reduction.
  • compound [28] can be obtained by reacting compound [27] with a reducing agent in a solvent under ice-cooling to room temperature.
  • Examples of the reducing agent to be used for the reaction include sodium borohydride and the like.
  • solvent to be used for the reaction examples include methanol, ethanol, 2-propanol, 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like.
  • Compound [29] can be obtained by converting the hydroxy of compound [28] to the leaving group L 2 in the same manner as in Production Method 2-1, Step 2-1-7.
  • Compound [30] can be obtained by reacting compound [29] with compound [17] in the same manner as in Production Method 2-1, Step 2-1-8.
  • Compound [31] can be obtained by removing P w of compound [30] in the same manner as in Production Method 2-1, Step 2-1-9.
  • Compound [32] can be obtained by reacting compound [31] with compound [20] in the same manner as in Production Method 2-1, Step 2-1-10.
  • Compound [32] can be indicated as compound [22].
  • Compound [I-al] can be obtained by converting alkoxy of compound [22] to hydroxy by hydrolysis in the same manner as in Production Method 1-1, Step 1-1-3.
  • R 1 , R 4 and n are as defined in the aforementioned formula [I], can be obtained by subjecting compound [9] to the reactions of Step 2-1-4, Step 2-1-5 and Step 2-1-11.
  • Step 2-1 the amide bond forming reaction is performed by using compound [10] and HNR b1 R b2 such as dimethylamine, tert-butylamine and the like and in the same manner as in Step 2-1-10. Thereafter, the resultant product is subjected to the reactions of Step 2-1-4, Step 2-1-5 and Step 2-1-11, whereby compound [I-a3] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • R 1 , R 4 , R b1 , R b2 and n are as defined in the aforementioned formula [I], can be obtained.
  • R 1 , R 4 and n are as defined in the aforementioned formula [I] can be obtained by subjecting compound [15] to the reaction of Step 2-1-11.
  • Step 2-1-11 the reaction of Step 2-1-11 is performed by using compound [15]. Thereafter, the resultant product is reacted with a C 1-6 alkyl-carboxylic anhydride such as acetic anhydride, propionic anhydride and the like, whereby compound [I-a5] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • R 1 , R 4 , R b3 , R b4 and n are as defined in the aforementioned formula [I], can be obtained.
  • R 1 , R 4 , R b8 , R b9 and n are as defined in the aforementioned formula [I], can be obtained.
  • R 1 , R 4 , R b14 , R b15 and n are as defined in the aforementioned formula [I], can be obtained.
  • R 1 , R 4 , R b6 , R b7 and n are as defined in the aforementioned formula [I], can be obtained.
  • R 1 , R 4 , R b16 , m2, m3 and m4 are as defined in the aforementioned formula [I] and C 1-6 Alkyl, L 2 , P v , t and Y are as defined in the above-mentioned Production Method 2-1.
  • Compound [36] can be obtained by reacting compound [34] with compound [35] in a solvent in the presence of a base.
  • Examples of the base to be used for the reaction include, lithium diisopropylamide, lithium bis(trimethylsilyl)amide and the like base.
  • solvent to be used for the reaction examples include ether solvents such as tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like, and a mixed solvent thereof.
  • ether solvents such as tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like
  • hydrocarbon solvents such as toluene, hexane, xylene and the like, and a mixed solvent thereof.
  • Compound [35] may be a commercially available product such as benzyl chloromethyl ether, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Compound [37] can be obtained by removing P v of compound [36] in the same manner as in Production Method 2-1, Step 2-1-6.
  • Compound [38] can be obtained by converting the ester of compound [37] to carboxy by hydrolysis in the same manner as in Production Method 2-1, Step 2-1-1.
  • Compound [39] can be obtained by reacting compound [38] with compound [19] in a solvent in the presence of a condensing agent and an additive in the same manner as in Production Method 2-1, Step 2-1-10.
  • Compound [40] can be obtained by cyclization of compound [39] by intramolecular Mitsunobu reaction.
  • compound [40] can be obtained by reacting compound [39] with an azodicarboxylic acid diester (e.g., diethyl azodicarboxylate, diisopropyl azodicarboxylate, bis(2-methoxyethyl) azodicarboxylate etc.) in a solvent in the presence of a phosphine such as triphenylphosphine, tributylphosphine and the like.
  • an azodicarboxylic acid diester e.g., diethyl azodicarboxylate, diisopropyl azodicarboxylate, bis(2-methoxyethyl) azodicarboxylate etc.
  • a phosphine such as triphenylphosphine, tributylphosphine and the like.
  • Examples of the solvent to be used for the reaction include dichloromethane, chloroform, 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether, toluene, N,N-dimethylformamide and the like. These may be used singly or as a mixture of two or more kinds thereof.
  • R 1 , R 4 and R c are as defined in the aforementioned formula [I].
  • R 1 , R 4 , R 5 , R c , m1 and X are as defined in the aforementioned formula [I]
  • Z is as defined in the above-mentioned Production Method 1-1
  • Hale and P v are as defined in the above-mentioned Production Method 2-1.
  • Compound [42] can be obtained by protecting the hydroxy group of compound [41] in the same manner as in Production Method 2-1, Step 2-1-3.
  • Compound [41] may be a commercially available product such as 2-bromo-3-methylphenol, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Compound [43] can be obtained by borating compound [42] in the same manner as in Production Method 1-3, Step 1-3.
  • Compound [44] can be obtained by the Suzuki coupling reaction of compound [3] and compound [43] in the same manner as in Production Method 1-1, Step 1-1-2.
  • Compound [45] can be obtained by removing P v of compound [44] in the same manner as in Production Method 2-1, Step 2-1-6.
  • Compound [47] can be obtained by the Mitsunobu reaction of compound [45] and compound [46].
  • compound [47] can be obtained by reacting compound [45] with compound [46] in a solvent in the presence of an azodicarboxylic acid diester (e.g., diethyl azodicarboxylate, diisopropyl azodicarboxylate, bis(2-methoxyethyl) azodicarboxylate etc.) and a phosphine such as triphenylphosphine, tributylphosphine and the like.
  • an azodicarboxylic acid diester e.g., diethyl azodicarboxylate, diisopropyl azodicarboxylate, bis(2-methoxyethyl) azodicarboxylate etc.
  • a phosphine such as triphenylphosphine, tributylphosphine and the like.
  • Examples of the solvent to be used for the reaction include dichloromethane, chloroform, 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether, toluene, N,N-dimethylformamide and the like. These may be used singly or as a mixture of two or more kinds thereof.
  • Compound [46] may be a commercially available product such as benzyl alcohol, 2-pyridinemethanol and the like, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Compound [I-b1] can be obtained by converting the alkoxy of compound [47] to hydroxy by hydrolysis in the same manner as in Production Method 1-1, Step 1-1-3.
  • R y is chloro or trifluoromethyl
  • compound [48] may be a commercially available product such as those shown below, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Compound [43a] can be obtained by reacting compound [49] with a boron compound in a solvent in the presence of a base.
  • compound [43a] can be obtained by adding a base to compound [49] in a solvent at ⁇ 78° C. to room temperature, and reacting the resultant product with a boron reagent at ⁇ 78° C. to room temperature.
  • Examples of the base to be used for the reaction include n-butyllithium, sec-butyllithium and the like.
  • Examples of the boron reagent to be used for the reaction include 2-isopropoxy-4,4,5,5-tetramethyl-1,3,2-dioxaborolane, trimethyl borate and the like.
  • solvent to be used for the reaction examples include tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like.
  • R 5 , R c , X and Cy are as defined in the aforementioned formula [I], m7 is 0, 1 or 2, and when m7 is 2, each R 5 is selected independently, can be obtained by appropriately converting the substituent of compound [2].
  • L 3 is a leaving group such as trifluoromethanesulfonyloxy and the like;
  • Compound [51] can be obtained by the Suzuki coupling reaction of compound [1] and compound [50] in the same manner as in Production Method 1-1, Step 1-1-1.
  • Compound [50] may be a commercially available product such as 4-(benzyloxy)phenylboronic acid, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Compound [52] can be obtained by the Suzuki coupling reaction of compound [4] and compound [51] in the same manner as in Production Method 1-1, Step 1-1-2.
  • Compound [53] can be obtained by removing the phenol protecting group P x of compound [52].
  • the deprotection can be performed by a known method according to the protecting group to be employed.
  • compound [52] when P x is benzyl, compound [52] only needs to be subjected to a hydrogenation reaction in a single or mixed solvent of tetrahydrofuran, ethyl acetate, ethanol, methanol, water and the like in the presence of a catalyst such as palladium carbon, platinum carbon and the like.
  • Compound [54] can be obtained by converting the hydroxy to a leaving group L 3 .
  • the leaving group is trifluoromethanesulfonyloxy
  • compound [54] can be obtained by reacting compound [53] with trifluoromethanesulfonic anhydride, N-phenyl bis(trifluoromethanesulfonimide) and the like in a solvent in the presence of a base from ice-cooling to room temperature.
  • Examples of the base to be used for the reaction include organic bases such as pyridine, 2,6-lutidine, triethylamine and the like; inorganic bases such as alkali metal salts (e.g., cesium carbonate, sodium hydride etc.) and the like.
  • organic bases such as pyridine, 2,6-lutidine, triethylamine and the like
  • inorganic bases such as alkali metal salts (e.g., cesium carbonate, sodium hydride etc.) and the like.
  • solvent to be used for the reaction examples include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like; halogenated solvents such as dichloromethane, chloroform and the like; polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile, pyridine and the like, and the like. These may be used singly or as a mixture of two or more kinds thereof.
  • ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like
  • hydrocarbon solvents such as toluene,
  • Compound [56] can be obtained by the Sonogashira reaction of compound [54] and compound [55].
  • compound [56] can be obtained by reacting compound [54] with compound [55] in a solvent preferably under heating in the presence of a base, a palladium catalyst and a copper catalyst.
  • Examples of the palladium catalyst to be used for the reaction include palladium acetate, tetrakistriphenylphosphinepalladium, bis(triphenylphosphine)palladium dichloride, (bis(diphenylphosphino)ferrocene)palladium dichloride-methylene chloride complex and the like.
  • Examples of the copper catalyst to be used for the reaction include copper iodide, copper bromide and the like.
  • Examples of the base to be used for the reaction include diethylamine, dicyclohexylamine, triethylamine, N-ethyldiisopropylamine and the like.
  • solvent to be used for the reaction examples include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like; and polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile, pyridine and the like. These may be used singly or as a mixture of two or more kinds thereof.
  • ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like
  • hydrocarbon solvents such as toluene, hexane, xylene and the like
  • polar solvents such as N,N
  • Compound [55] may be a commercially available product such as cyclohexylacetylene, 2-ethynylpyridine and the like, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Compound [I-c1] can be obtained by converting the alkoxy of compound [56] to hydroxy by hydrolysis in the same manner as in Production Method 1-1, Step 1-1-3.
  • a hydrogenation reaction only needs to be performed in a single or mixed solvent of tetrahydrofuran, ethyl acetate, ethanol, methanol, water and the like in the presence of a catalyst such as palladium carbon or platinum carbon and the like.
  • the reaction mixture was stirred for 20 min and at room temperature for 20 hr. Thereafter, to the reaction mixture were added 6-methyl-2-pyridinemethanol (0.099 g, 0.80 mmol) and triphenylphosphine (0.21 g, 0.80 mmol), and bis(2-methoxyethyl) azodicarboxylate (0.19 g, 0.80 mmol) in 2 portions under ice-cooling. After stirring for 20 min, the reaction mixture was stirred for 10 min at room temperature. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned.
  • a solution of the residue in N,N-dimethylformamide (2.0 ml) was added to a solution of di-tert-butyl iminodicarboxylate (0.140 g, 0.64 mmol) and sodium hydride (0.026 g, 60 wt % oil dispersion) in N,N-dimethylformamide (1.0 ml) under ice-cooling, and the mixture was stirred at room temperature for 15 min.
  • the reaction mixture were added water and ethyl acetate, and the mixture was partitioned.
  • the reaction mixture was added to a solution of di-tert-butyl iminodicarboxylate (0.32 g, 1.5 mmol) and cesium carbonate (1.2 g, 3.6 mmol) in N,N-dimethylformamide (3.0 ml) at room temperature, and the mixture was stirred for 1 hr.
  • reaction mixture was stirred at room temperature for 1 hr, and 1.9M diethyl azodicarboxylate/toluene solution (0.028 ml, 0.053 mmol) was added.

Abstract

wherein each symbol is as defined in the SPECIFICATION.

Description

    TECHNICAL FIELD OF THE INVENTION
  • The present invention relates to a triazine compound having a microsomal prostaglandin E2 synthase-1 (mPGES-1) inhibitory activity or a pharmaceutically acceptable salt thereof, a pharmaceutical composition containing same, pharmaceutical use thereof and the like.
    • BACKGROUND OF THE INVENTION
  • Non-steroidal anti-inflammatory drugs (NSAIDs) are often used for the treatment of diseases accompanying inflammation, fever and pain, for example, rheumatism, osteoarthritis, headache and the like. NSAIDs show an anti-inflammatory action, an antipyretic action and an analgesic action by preventing production of prostanoids by inhibiting cyclooxygenase (COX).
  • COX includes two isoforms of COX-1 which is ubiquitously distributed and constitutively expressed, and COX-2 which expression is induced by various pro-inflammatory stimulations, for example, cytokines such as interleukin-1β (IL-1β) and the like. COX-1 and COX-2 are enzymes that convert arachidonic acid derived from cell membrane phospholipids to prostaglandin H2 (PGH2) which is a prostanoid precursor. Specific prostanoid synthases are responsible for the conversion of PGH2 to respective prostanoids (prostaglandin E2 (PGE2), prostaglandin F2α (PGF2α), prostaglandin I2 (PGI2), prostaglandin D2 (PGD2), thromboxane A2 (TXA2) etc.). These prostanoids have various physiological activities, for example, induction/suppression of inflammation, vasodilation/vasoconstriction, bronchodilation/bronchoconstriction, induction of/awakening from sleep, development of fever and the like. PGE2 is the most commonly existing prostaglandin in living organisms, and is known to be deeply involved in inflammation, pain and fever. Therefore, suppression of PGE2 production is considered the main action mechanism of NSAIDs.
  • Inhibition of COX-1 or COX-2 suppresses all prostanoids production in the downstream thereof. This is considered to cause side effects of NSAIDs. Since NSAIDs that non-selectively inhibit COX also suppress production of PGE2 by COX-1 and PGE2 protectively acts on stomach mucosal injury, NSAIDs are considered to suppress secretion of gastric mucus and gastric mucosal blood flow, thereby increasing the risk of stomach perforations, bleeding and the like. While COX-2 selective inhibitors suppress production of PGI2 having a vasodilation action and a platelet aggregation inhibitory action in vascular endothelial cells, they do not suppress production of TXA2 which is a blood coagulation factor produced by platelet COX-1. Therefore, they are considered to disrupt the balance of the blood coagulation system to increase the risk of cardiovascular disorder.
  • Microsomal prostaglandin E2 synthase-1 (mPGES-1) is an enzyme that catalyzes the final step of PGE2 biosynthesis, and belongs to the membrane-associated proteins in eicosanoid and glutathione metabolism family (MAPEG family). The human mPGES-1 gene was cloned in 1999, and indicated to be constitutively expressed in placenta, prostate, testis and mammary gland (non-patent document 1). In other organs, human mPGES-1 gene expression is induced by various pro-inflammatory stimulations, conjugated with COX-2. For example, inflammatory cytokine IL-1β and Tumor Necrosis Factor-α (TNF α) induce mPGES-1 expression in synovial cell, osteoblast, endothelial cell, orbital fibroblast, gingival cell, chondrocyte, endothelial cell, myocardial cell and the like. For example, Lipopolysaccharide (LPS), which is a bacterial endotoxin, induces mPGES-1 expression in macrophage, smooth muscle and the like.
  • mPGES-1 inhibitor is considered to selectively suppress PGE2 production only in the topical site of inflammation or tissues where mPGES-1 is expressed, and does not suppress production of prostanoids (PGI2, PGD2, PGF2α, TXA2 etc.) other than PGE2 (non-patent documents 2, 3). Therefore, mPGES-1 inhibitor is considered to be a medicament having an efficacy equivalent to that of NSAIDs but free of side effects of NSAIDs derived from a decreased production of prostanoids other than PGE2.
  • It is also known that when one of the metabolism pathways downstream from PGH2 is shut off in the arachidonic acid cascade, PGH2 is converted to prostanoids other than the shut-off pathway, or shunt occurs. That is, it is known that while the production amount of PGE2 in macrophage derived from mPGES-1 knockout mice stimulated with LPS becomes lower than the PGE2 production amount in macrophage derived from wild-type (WT) mice stimulated with LPS, the production amounts of TXB2, PGI2, PGD2 and PGF2α in macrophage derived from mPGES-1 knockout mice stimulated with LPS increase beyond the production amounts thereof in macrophage derived from WT mice stimulated with LPS (non-patent document 4). Since mPGES-1 inhibitor increases production of other prostanoids while suppressing the PGE2 production, it is considered to be effective even for diseases different from those treated by NSAIDs.
  • Use of mPGES-1 inhibitor is described below.
    • (1) Pain
  • In mPGES-1 knockout mice, intraperitoneal PGE2 production amount and nociceptive response per unit time significantly decrease as compared to WT mice, in the evaluation of nociceptive response by LPS stimulation which is an acute inflammatory pain model. Therefore, mPGES-1 inhibitor is considered to be an analgesic for acute inflammatory pain (non-patent documents 3, 6).
    • (2) Rheumatism
  • mPGES-1 gene of Swedish females contains some single nucleotide polymorphisms that increase the onset risk and severity of rheumatism. An increase in the mPGES-1 expression is immunohistologically confirmed in the synovium of rheumatism patients showing single nucleotide polymorphism (Reference SNP ID number: r523202821) that increases severity, as compared to patients free of mutation (non-patent document 5). In mPGES-1 knockout mice, intraarticular infiltration of inflammatory cells, articular destruction and tumentia of the four limbs are markedly suppressed in a collagen-induced arthritis model, which is an animal model of rheumatism, as compared to WT mice (non-patent document 6). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug for rheumatism.
    • (3) Osteoarthritis
  • mRNA expression of mPGES-1 increases in meniscus cells of osteoarthritis patients (non-patent document 7). mPGES-1 inhibitor reduces nociceptive responses in osteoarthritis model using monoiodoacetic acid, as compared to WT mice (patent document 1). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug for osteoarthritis.
    • (4) Fever
  • In mPGES-1 knockout mice, body temperature elevation due to LPS stimulation is suppressed as compared to WT mice (non-patent document 8). Therefore, mPGES-1 inhibitor is considered to be an antipyretic drug.
    • (5) Alzheimer's Disease
  • Long-term use of NSAIDs mitigates the onset and progression of Alzheimer's disease. Under amyloid β peptide treatment, PGE2 production in the primary culture brain neuron of mPGES-1 knockout mice is suppressed, compared to the brain neuron of WT mice, and nerve cell death does not occur (non-patent document 9). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug for Alzheimer's disease.
    • (6) Multiple Sclerosis
  • EP4 gene of multiple sclerosis patients contains some single nucleotide polymorphisms that increase the onset risk (Reference SNP ID numbers: rs9292777, rs4613763, rs1044063, r56896969). In macrophage present in the periventricular demyelinating lesion of multiple sclerosis patients, expression of mPGES-1 protein is confirmed. In mPGES-1 knockout mice, PGE2 production in the spinal cord of experimental autoimmune encephalomyelitis model mice, which is an animal model of multiple sclerosis, is suppressed, and progression of paralysis is suppressed, as compared to WT mice, (non-patent document 10). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug for multiple sclerosis.
    • (7) Arteriosclerosis
  • In mPGES-1 knockout mice, PGE2 production in vascular endothelial cells of high-fat fed low density lipoprotein (LDL) receptor deficient mice, which is an atherosclerosis model, decreases, and atheroma formation is delayed as compared to WT mice. In vascular endothelial cells, production of PGI2, which is known to have a platelet function suppressive action, increases (non-patent document 11). Therefore, mPGES-1 inhibitor is considered to be a prophylactic or therapeutic drug for arteriosclerosis.
    • (8) Glaucoma, Ocular Hypertension
  • Glaucoma is a disease showing a characteristic change in the optic nerve and the field of vision. Optic nerve disorder can be generally improved or suppressed by sufficiently decreasing the intraocular pressure. Glaucoma can be categorized into open angle glaucoma and closed angle glaucoma.
  • mPGES-1 gene is constitutively highly expressed in human conjunctiva (GEO accession No: GSE2513 (Gene Expression Omnibus:http://www.ncbi.nlm.nih.gov/geo/)). In the retina of glaucoma patients, expression of mPGES-1 increases as compared to healthy individuals. In the retina of high intraocular pressure dogs and high intraocular pressure mice, which are glaucoma models, expression of mPGES-1 increases as compared to normal animals (GEO accession No: human GSE2378, dog GSE21879, mouse GSE3554).
  • When PGE2 is instilled into the eyes of healthy individuals, the intraocular pressure increases, along with the expansion of blood vessels, for 2 hours after instillation (non-patent document 12). When PGE2 is administered to rabbits subconjunctivally, the intraocular pressure increases due to dilatation of ciliary body and increase in the aqueous humor production (non-patent document 13). PGF2α and PGD2, which are prostaglandins that may increase when mPGES-1 is inhibited, decrease the intraocular pressure of rabbit (non-patent document 14). PGF2α formulations increase outflow of aqueous humor and are used as therapeutic drugs for glaucoma that decrease the intraocular pressure. PGI2 does not show a clear action on the intraocular pressure of rabbits. That is, the intraocular pressure is considered to decrease since decrease of PGE2 suppresses aqueous humor production by mPGES-1 inhibition, and/or since increased PGD2 and PGF2α promote outflow of aqueous humor due to shunt. Also, PGE2 promotes expression of vascular endothelial growth factor (VEGF) from retina (non-patent document 15). Since VEGF produced in retina transfers to the anterior ocular segment to cause angiogenesis glaucoma, which is increase of the intraocular pressure that is caused by obstruction of corner angle due to angiogenesis in iris, mPGES-1 inhibitor is considered to show an improvement or prophylactic effect on angiogenesis glaucoma as well. Furthermore, considering an anti-inflammatory action by the inhibition of PGE2 production, mPGES-1 inhibitor is applicable to patients having intraocular inflammation, who require careful administration of the existing prostaglandin formulations (latanoprost etc.). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug also effective for glaucoma having various background diseases.
    • (9) Ischemic Retinal Disease
  • Excessive secretion of VEGF plays a key role in ischemic retinal diseases such as diabetic retinopathy, diabetic macular edema, retinal vein occlusion and the like. Since PGE2 promotes expression of VEGF (non-patent document 15), mPGES-1 inhibitor is considered to improve these diseases.
    • (10) Systemic Scleroderma
  • Expression of mPGES-1 increases in the skin of systemic scleroderma patients, as compared to healthy individuals. Similarly, expression of mPGES-1 increases in the skin of bleomycin induced scleroderma model mice, which is a systemic scleroderma model, as compared to the skin of normal mice. As compared to WT mice, mPGES-1 knockout mice showed a decrease in the accumulation of macrophage in the dermal lesion of bleomycin induced scleroderma model mice, and mitigation of cutaneous thickening, deposition of extracellular matrix and increase in the collagen content (non-patent document 16). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug for systemic scleroderma.
    • (11) Cancer
  • In mPGES-1 knockout mice, the polyp number and size were markedly suppressed in azoxymethane-induced colorectal cancer model mice, which are animal model of colorectal cancer, as compared to WT mice. In mPGES-1 knockout mice, PGE2 production in large intestinal tumor tissue decreased and production amount of PGI2 that inhibits adhesion of cancer cells and PGD2 that induces cell death via peroxisome proliferator-activated receptor γ(PPARγ) increased, as compared to WT mice. When colorectal cancer or lung cancer cells were transplanted into the spleen of mPGES-1 knockout mice, the post-transplantation weight of spleen tumor and the rate of metastasis to the liver decreased as compared to WT mice. Growth of lung cancer cells was decreased when they ware co-cultured in vitro with mPGES-1 knockout mice-derived bone marrow macrophages compared to when they ware co-cultured with WT mice-derived bone marrow macrophages, which indicates that host macrophage-derived PGE2 is involved in cancer cell growth (non-patent document 17). Therefore, mPGES-1 inhibitor is considered to be an anticancer drug that suppresses the growth and metastasis of cancer including colorectal cancer.
  • (12) Disease for which Suppression of PGE2 Production is Effective
  • As inflammatory symptoms and/or pain relating to the conditions thereof, for which NSAIDs are effective, for example, arthritis, gout, nephrolithiasis, urolithiasis, headache, menstrual pain, toothache, lumbago, muscular pain, periarthritis scapulohumeralis, cervical syndrome, temporomandibular disorder, and postoperative or posttraumatic inflammation and pain, and inflammation and pain after tooth extraction can be mentioned. Besides these, acute and chronic non-bacterial inflammation of eye can be mentioned and, for example, uveitis, allergic conjunctivitis and postoperative inflammation and ophthalmalgia in intraocular operation can be mentioned.
  • The main mechanism for the efficacy of NSAIDs is considered to be the suppression of PGE2 production, which is an inflammation promoting substance. Since mPGES-1 inhibitor also has a suppressive action on the PGE2 production, it is considered to be a therapeutic drug for these diseases.
  • The mPGES-1 inhibitor is considered to be beneficial for the prophylaxis or treatment of pain, rheumatism, osteoarthritis, fever, Alzheimer's disease, multiple sclerosis, arteriosclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer including colorectal cancer and diseases for which suppression of PGE2 production is effective.
    • DOCUMENT LIST Patent Document
    • patent document 1: WO 2012/161965
    Non-Patent Documents
    • non-patent document 1: JAKOBSSON, P J et al. Identification of human prostaglandin E synthase: a microsomal, glutathione-dependent, inducible enzyme, constituting a potential novel drug target. Proc Natl Acad Sci USA. Jun. 22, 1999, Vol. 96, No. 13, pages 7220-7225.
    • non-patent document 2: SAMUELSSON, B et al. Membrane prostaglandin E synthase-1: a novel therapeutic target. Pharmacol Rev. September 2007, Vol. 59, No. 3, pages 207-224.
    • non-patent document 3: KAMEI, D et al. Reduced pain hypersensitivity and inflammation in mice lacking microsomal prostaglandin e synthase-1. J Biol Chem. Aug. 6, 2004, Vol. 279, No. 32, pages 33684-33695.
    • non-patent document 4: TREBINO, C E et al. Redirection of eicosanoid metabolism in mPGES-1-deficient macrophages. J Biol Chem. Apr. 29, 2005, Vol. 280, No. 17, pages 16579-16585.
    • non-patent document 5: KOROTKOVA, M et al. Variants of gene for microsomal prostaglandin E2 synthase show association with disease and severe inflammation in rheumatoid arthritis. Eur J Hum Genet. August 2011, Vol. 19, No. 8, pages 908-914.
    • non-patent document 6: TREBINO, C E et al. Impaired inflammatory and pain responses in mice lacking an inducible prostaglandin E synthase. Proc Natl Acad Sci USA. Jul. 22, 2003, Vol. 100, No. 15, pages 9044-9049.
    • non-patent document 7: SUN, Y et al. Analysis of meniscal degeneration and meniscal gene expression. BMC Musculoskelet Disord. 2010, Vol. 11, pages 19.
    • non-patent document 8: ENGBLOM, D et al. Microsomal prostaglandin E synthase-1 is the central switch during immune-induced pyresis. Nat Neurosci. November 2003, Vol. 6, No. 11, pages 1137-1138.
    • non-patent document 9: KUROKI, Y et al. Deletion of microsomal prostaglandin E synthase-1 protects neuronal cells from cytotoxic effects of beta-amyloid peptide fragment 31-35. Biochem Biophys Res Commun. Aug. 3, 2012, Vol. 424, No. 3, pages 409-413.
    • non-patent document 10: KIHARA, Y et al. Targeted lipidomics reveals mPGES-1-PGE2 as a therapeutic target for multiple sclerosis. Proc Natl Acad Sci USA. Dec. 22, 2009, Vol. 106, No. 51, pages 21807-21812.
    • non-patent document 11: WANG, M et al. Deletion of microsomal prostaglandin E synthase-1 augments prostacyclin and retards atherogenesis. Proc Natl Acad Sci USA. Sep. 26, 2006, Vol. 103, No. 39, pages 14507-14512.
    • non-patent document 12: FLACH, A J et al. Topical prostaglandin E2 effects on normal human intraocular pressure. J Ocul Pharmacol. Spring 1988, Vol. 4, No. 1, pages 13-18.
    • non-patent document 13: NAKAJIMA, T et al. [Effects of prostaglandin E2 on intraocular pressure, anterior chamber depth and blood flow volume of the iris and the ciliary body in rabbit eyes]. Nihon Ganka Gakkai Zasshi. April 1992, Vol. 96, No. 4, pages 455-461.
    • non-patent document 14: GOH, Y et al. Prostaglandin D2 reduces intraocular pressure. Br J Ophthalmol. June 1988, Vol. 72, No. 6, pages 461-464.
    • non-patent document 15: YANNI, S E et al. The role of PGE2 receptor EP4 in pathologic ocular angiogenesis. Invest Ophthalmol Vis Sci. November 2009, Vol. 50, No. 11, pages 5479-5486.
    • non-patent document 16: MCCANN, M R et al. mPGES-1 null mice are resistant to bleomycin-induced skin fibrosis. Arthritis Res Ther. 2011, Vol. 13, No. 1, pages R6.
    • non-patent document 17: SASAKI, Y et al. Microsomal prostaglandin E synthase-1 is involved in multiple steps of colon carcinogenesis. Oncogene. Jun. 14, 2012, Vol. 31, No. 24, pages 2943-2952.
    SUMMARY OF THE INVENTION
  • The present invention aims to provide a triazine compound having an mPGES-1 inhibitory activity or a pharmaceutically acceptable salt thereof, a pharmaceutical composition containing same, and pharmaceutical use thereof and the like. As the target disease, for example, pain, rheumatism, osteoarthritis, fever, Alzheimer's disease, multiple sclerosis, arteriosclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer including colorectal cancer and diseases for which suppression of PGE2 production is effective can be mentioned.
  • The present inventors have found a triazine compound having an mPGES-1 inhibitory activity, which is represented by the following formula [I], and completed the present invention.
  • Accordingly, the present invention is as follows.
  • [01]
  • A compound represented by the formula [I] or a pharmaceutically acceptable salt thereof:
  • Figure US20200087266A1-20200319-C00002
    • wherein
    • X is CH or N,
    • ring Cy is
    • the formula:
  • Figure US20200087266A1-20200319-C00003
    • or
    • the formula:
  • Figure US20200087266A1-20200319-C00004
    • {wherein R1 is
  • (1) halogen,
  • (2) C1-6 alkyl,
  • (3) cyano or
  • (4) haloC1-4 alkyl,
  • R2 is
  • (1) halogen,
  • (2) hydroxy,
  • (3) carboxy,
  • (4) C1-6 alkyl,
  • (5) C1-6 alkoxy,
  • (6) haloC1-4 alkoxy,
  • (7) haloC1-4 alkyl,
  • (8) C1-6 alkyl-carbonyl,
  • (9) —C(O)NRa1Ra2 (Ra1 and Ra2 are each independently hydrogen or C1-6 alkyl) or
  • (10) —(CnH2n)—Rb
  • (n is 1, 2, 3 or 4, —(CnH2n)— may be straight or branched chain, and
  • Rb is
      • (a) hydroxy,
      • (b) carboxy,
      • (c) C1-6 alkoxy,
      • (d) C1-6 alkyl-carbonyloxy,
      • (e) —C(O)NRb1Rb2 (Rb1 and Rb2 are each independently hydrogen or C1-6 alkyl),
      • (f) —OC(O)NRb3Rb4 (Rb3 and Rb4 are each independently hydrogen or C1-6 alkyl),
      • (g) —NRb5C(O)NRb6Rb7 (Rb6, Rb6 and Rb7 are each independently hydrogen or C1-6 alkyl),
      • (h) —NRb8Rb9 (Rb8 and Rb9 are each independently hydrogen, C1-6 alkyl or haloC1-4 alkyl),
      • (i) —NRb10S(O)2Rb11 (Rb10 and Rb11 are each independently hydrogen, C1-6 alkyl or C3-7 cycloalkyl),
      • (j) —NRb12C(O)ORb13 (Rb12 is hydrogen or C1-6 alkyl, and Rb13 is C1-6 alkyl),
      • (k) —NRb14C(O)Rb15 (Rb14 is hydrogen or C1-6 alkyl, and Rb15 is
        • (i) C6-10 aryl,
        • (ii) C1-8 alkyl (said C1-8 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of hydroxy, haloC1-4 alkyl, C1-6 alkoxy and C6-10 aryl),
        • (iii) adamantyl or
        • (iv) C3-7 cycloalkyl (said C3-7 cycloalkyl is optionally substituted by 1, 2, 3 or 4 substituents selected from the group consisting of C1-6 alkyl, halogen, hydroxyl C1-6 alkyl and halo C1-4 alkyl, and/or optionally form a fused ring with a benzene ring), or
  • Rb14 and Rb15 optionally form a 4-, 5- or 6-membered lactam together with the nitrogen atom the nitrogen atom that Rb14 is bonded to and the carbon atom that Rb15 is bonded to (said lactam is optionally substituted by 1, 2 or 3 C1-6 alkyls, and/or optionally form a fused ring with a benzene ring),
  • (1) the formula:
  • Figure US20200087266A1-20200319-C00005
    • wherein m2 and m3 are each independently 1, 2 or 3, m4 is 0, 1, 2, 3 or 4, Rb16 is C1-6 alkyl or C1-6 alkoxy, and when m4 is 2, 3 or 4, each Rb16 is selected independently, or
  • (m) the formula:
  • Figure US20200087266A1-20200319-C00006
    • wherein m5 and m6 are each independently 1, 2 or 3, and Rb17 is C1-6 alkyl or C1-6 alkoxy)),
  • R3 is
  • (1) halogen,
  • (2) hydroxy,
  • (3) C1-6 alkyl or
  • (4) —ORc {Rc is C1-6 alkyl optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (a) to (f);
      • (a) halogen,
      • (b) hydroxy,
      • (c) C1-6 alkoxy,
      • (d) —C(O)NRc1Rc2 (Rc1 and Rc2 are each independently hydrogen or C1-6 alkyl),
      • (e) C6-10 aryl (said C6-10 aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
        • (i) halogen,
        • (ii) hydroxy,
        • (iii) C1-6 alkyl,
        • (iv) C1-6 alkoxy, and
        • (v) haloC1-4 alkyl), and
      • (f) 5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms (said heteroaryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
        • (i) halogen,
        • (ii) hydroxy,
        • (iii) C1-6 alkyl,
        • (iv) C1-6 alkoxy, and
        • (v) haloC1-4 alkyl)}, and
  • R4 is
  • (1) hydrogen,
  • (2) halogen,
  • (3) C1-6 alkyl or
  • (4) C1-6 alkoxy},
  • R5 is
  • (1) halogen,
  • (2) hydroxy,
  • (3) C1-6 alkylsulfanyl,
  • (4) C1-6 alkyl (said C1-6 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of halogen, C6-10 aryl and C1-6 alkoxy),
  • (5) C3-7 cycloalkyl,
  • (6) —ORd {Rd is
      • (a) C2-6 alkynyl,
      • (b) C3-7 cycloalkyl optionally substituted by 1, 2 or 3 C1-6 alkyls or
      • (c) C1-8 alkyl (said C1-8 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (i) to (v);
        • (i) halogen,
        • (ii) C6-10 aryl,
        • (iii) C1-6 alkoxy,
        • (iv) C3-7 cycloalkyl (said C3-7 cycloalkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C1-6 alkyl and haloC1-4 alkyl), and
        • (v) 4-, 5- or 6-membered saturated heterocyclyl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms (said saturated heterocyclyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C1-6 alkyl and haloC1-4 alkyl))} or
  • (7) the formula:
  • Figure US20200087266A1-20200319-C00007
    • wherein Re is
      • (a) C1-6 alkyl,
      • (b) C3-7 cycloalkyl,
      • (c) 5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms, or
      • (d) C6-10 aryl (said C6-10 aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
        • (i) halogen,
        • (ii) C1-6 alkyl,
        • (iii) haloC1-4 alkyl,
        • (iv) C1-6 alkoxy, and
        • (v) haloC1-4 alkoxy), and
    • m1 is 0, 1, 2 or 3 and, when m1 is 2 or 3, each R5 is selected independently,
    • excluding 4,6-bis-(2,5-dimethyl-phenyl)-1,3,5-triazin-2-ol.
      [02]
  • The compound of [01] or a pharmaceutically acceptable salt thereof, wherein ring Cy is the formula:
  • Figure US20200087266A1-20200319-C00008
    • wherein R1, R2 and R4 are as defined in [01].
      [03]
  • The compound of [01] or a pharmaceutically acceptable salt thereof, wherein ring Cy is the formula:
  • Figure US20200087266A1-20200319-C00009
    • wherein R1, R3 and R4 are as defined in [01].
      [04]
  • The compound of any of [01] to [03] or a pharmaceutically acceptable salt thereof, wherein X is CH.
  • [05]
  • The compound of any of [01] to [03] or a pharmaceutically acceptable salt thereof, wherein X is N.
  • [06]
  • The compound of any of [01] to [05] or a pharmaceutically acceptable salt thereof, wherein R1 is
  • (1) chloro,
  • (2) methyl,
  • (3) cyano or
  • (4) trifluoromethyl.
  • [07]
  • The compound of any of [01] to [06] or a pharmaceutically acceptable salt thereof, wherein R4 is hydrogen.
  • [08]
  • The compound of any of [01], [02] and [04] to [07] or a pharmaceutically acceptable salt thereof, wherein R2 is —(CnH2n)—Rb (n is 1 or 2, —(CnH2n)— may be straight or branched chain, and Rb is
  • (a) —C(O)NRb1Rb2,
  • (b) —NRb5C(O)NRb6Rb7,
  • (c) —NRb10S(O)2Rb11 or
  • (d) —NRb14C(O)Rb15
  • (Rb1, Rb2, Rb5, Rb6, Rb7, Rb10, Rb11, Rb14, and Rb15 are as defined in [01])).
    [09]
  • The compound of [08] or a pharmaceutically acceptable salt thereof, wherein R2 is —CH2—Rb (Rb is as defined in [08]).
  • [10]
  • The compound of any of [01] and [03] to [09] or a pharmaceutically acceptable salt thereof, wherein R3 is
  • (1) halogen,
  • (2) hydroxy,
  • (3) C1-6 alkyl or
  • (4) —ORc {Rc is C1-6 alkyl optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (a) to (f)
      • (a) halogen,
      • (b) hydroxy,
      • (c) C1-6 alkoxy,
      • (d) —C(O)NRc1Rc2 (Rc1 and Rc2 are each independently hydrogen or C1-6 alkyl),
      • (e) phenyl (said phenyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
        • (i) halogen,
        • (ii) hydroxy,
        • (iii) C1-6 alkyl,
        • (iv) C1-6 alkoxy, and
        • (v) haloC1-4 alkyl), and
      • (f) pyridyl (said pyridyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
        • (i) halogen,
        • (ii) hydroxy,
        • (iii) C1-6 alkyl,
        • (iv) C1-6 alkoxy, and
        • (v) haloC1-4 alkyl)}.
          [11]
  • The compound of any of [01] to [10] or a pharmaceutically acceptable salt thereof, wherein m1 is 1, and
  • R5 is the formula:
  • Figure US20200087266A1-20200319-C00010
    • wherein Re is as defined in [01].
      [12]
  • A compound selected from the following formulas:
  • Figure US20200087266A1-20200319-C00011
    Figure US20200087266A1-20200319-C00012
    Figure US20200087266A1-20200319-C00013
    Figure US20200087266A1-20200319-C00014
    Figure US20200087266A1-20200319-C00015
    Figure US20200087266A1-20200319-C00016
    • or a pharmaceutically acceptable salt thereof.
      [13]
  • A pharmaceutical composition comprising the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
  • [14]
  • An mPGES-1 inhibitor comprising the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof.
  • [15]
  • A therapeutic or prophylactic agent for pain, rheumatism, fever, osteoarthritis, arteriosclerosis, Alzheimer's disease, multiple sclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer or a disease for which suppression of PGE2 production is effective, comprising the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof.
  • [16]
  • A therapeutic or prophylactic agent for glaucoma or ocular hypertension, comprising the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof, and one or more kinds of other therapeutic agents for glaucoma in combination.
  • [17]
  • A method of inhibiting mPGES-1, comprising administering a pharmaceutically effective amount of the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof to a human.
  • [18]
  • A method of treating or preventing pain, rheumatism, fever, osteoarthritis, arteriosclerosis, Alzheimer's disease, multiple sclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer or a disease for which suppression of PGE2 production is effective, which method comprising administering a pharmaceutically effective amount of the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof to a human.
  • [19]
  • The method of [18] for treating or preventing glaucoma or ocular hypertension, further comprising administering a pharmaceutically effective amount of one or more kinds of other therapeutic agents for glaucoma to the human.
  • [20]
  • Use of the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof for the production of an mPGES-1 inhibitor.
  • [21]
  • Use of the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof for the production of a therapeutic or prophylactic agent for pain, rheumatism, fever, osteoarthritis, arteriosclerosis, Alzheimer's disease, multiple sclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer or a disease for which suppression of PGE2 production is effective.
    • EFFECT OF THE INVENTION
  • The compound of the present invention is effective as a therapeutic or prophylactic agent for pain, rheumatism, fever, osteoarthritis, arteriosclerosis, Alzheimer's disease, multiple sclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer including colorectal cancer, a disease for which suppression of PGE2 production is effective and the like.
    • BRIEF DESCRIPTION OF THE DRAWINGS
  • FIG. 1 shows effect of a test article (compounds of Example 2-98), a reference article (Xalatan (registered trademark)) or a vehicle (methylcellulose, MC) on the intraocular pressure immediately before and after administration in Macaca fascicularis.
    •  DESCRIPTION OF EMBODIMENTS
  • The definitions of the terms used in the present invention are as follows.
  • The “halogen” is fluoro, chloro, bromo or iodo.
  • The “C1-6 alkyl” means straight chain or branched chain alkyl having 1 to 6 carbon atoms. Examples thereof include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, isopentyl, neopentyl, 1-ethylpropyl, hexyl, isohexyl, 1,1-dimethylbutyl, 2,2-dimethylbutyl, 3,3-dimethylbutyl, 2-ethylbutyl and the like.
  • The “C1-8 alkyl” means straight chain or branched chain alkyl having 1 to 8 carbon atoms. Examples thereof include methyl, ethyl, propyl, isopropyl, 1,1-dimethylpropyl, 1-ethyl-propyl, 1-methyl-1-ethyl-propyl, butyl, isobutyl, sec-butyl, tert-butyl, 1-methyl-1-propyl-butyl, pentyl, isopentyl, neopentyl, 1-ethylpropyl, hexyl, isohexyl, 1,1-dimethylbutyl, 2,2-dimethylbutyl, 3,3-dimethylbutyl, 2-ethylbutyl and the like.
  • The “C1-6 alkoxy” means alkoxy wherein the alkyl moiety is the above-defined “C1-6 alkyl”. Examples thereof include methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, sec-butoxy, tert-butoxy, pentyloxy, isopentyloxy, neopentyloxy, 1,2-dimethylpropyloxy, 1-ethylpropyloxy, hexyloxy, isohexyloxy, 1,2,2-trimethylpropyloxy, 1,1-dimethylbutyloxy, 2,2-dimethylbutyloxy, 3,3-dimethylbutyloxy, 2-ethylbutyloxy and the like.
  • The “haloC1-4 alkyl” means straight chain or branched chain alkyl having 1-4 carbon atoms, which is substituted by 1 to 9 the above-defined “halogens”. When it is substituted by plural halogens, respective halogens may be the same or different. Examples thereof include 2-fluoroethyl, 2-chloroethyl, 2-bromoethyl, 3-fluoropropyl, 3-chloropropyl, 4-fluorobutyl, 4-chlorobutyl, 1,1-difluoroethyl, 1,1-difluoropropyl, 1,1-difluoro-2-methylpropyl, trifluoromethyl, 2,2,2-trifluoroethyl, 3,3,3-trifluoropropyl, 4,4,4-trifluorobutyl, pentafluoroethyl, 2,2,2-trifluoro-1-trifluoromethyl-ethyl and the like.
  • The “haloC1-4 alkoxy” means alkoxy wherein the alkyl moiety is the above-defined “haloC1-4 alkyl”. Examples thereof include fluoromethoxy, chloromethoxy, bromomethoxy, 2-fluoroethoxy, 2-chloroethoxy, 2-bromoethoxy, 3-fluoropropoxy, 3-chloropropoxy, 4-fluorobutoxy, 4-chlorobutoxy, 1,1-difluoroethoxy, 2,2-difluoroethoxy, 1,1-difluoropropoxy, 2,2-difluoropropoxy, 3,3-difluoropropoxy, 1,1-difluoro-2-methylpropoxy, trifluoromethoxy, 2,2,2-trifluoroethoxy, 3,3,3-trifluoropropoxy, 4,4,4-trifluorobutoxy, pentafluoroethoxy, 2,2,2-trifluoro-1-trifluoromethyl-ethoxy and the like.
  • The “hydroxyC1-6 alkyl” means the above-defined “C1-6 alkyl” substituted by 1 or 2 hydroxy. Examples thereof include hydroxymethyl, 2-hydroxyethyl, 1-hydroxy-1-methylethyl, 1,2-dihydroxyethyl, 3-hydroxypropyl, 1-hydroxy-2,2-dimethylpropyl, 4-hydroxybutyl, 1-hydroxy-2,2-dimethylbutyl, 5-hydroxypentyl, 6-hydroxyhexyl and the like.
  • The “C1-6 alkyl-carbonyl” means carbonyl bonded to the above-defined “C1-6 alkyl”. Examples thereof include acetyl, propionyl, 2,2-dimethylpropionyl, butyryl, 3-methylbutyryl, 2,2-dimethylbutyryl, pentanoyl, 4-methylpentanoyl, hexanoyl and the like.
  • The “C1-6 alkyl-carbonyloxy” means carbonyloxy bonded to the above-defined “C1-6 alkyl”. Examples thereof include methylcarbonyloxy, ethylcarbonyloxy, propylcarbonyloxy, isopropylcarbonyloxy, butylcarbonyloxy, isobutylcarbonyloxy, sec-butylcarbonyloxy, tert-butylcarbonyloxy, pentylcarbonyloxy, isopentylcarbonyloxy, 2-methylbutylcarbonyloxy, 1,1-dimethylpropylcarbonyloxy, neopentylcarbonyloxy, 3,3-dimethylbutylcarbonyloxy, 1-ethylpropylcarbonyloxy, hexylcarbonyloxy and the like.
  • The “C3-7 cycloalkyl” means 3- to 7-membered monocyclic cycloalkyl. Examples thereof include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and cycloheptyl.
  • The “C6-10 aryl” means 6- to 10-membered aryl. Examples thereof include phenyl, 1-naphthyl, 2-naphthyl and the like. Of these, preferred is phenyl.
  • The “5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms” means 5- or 6-membered monocyclic heteroaryl containing, besides carbon atoms, 1, 2 or 3 hetero atoms selected from a nitrogen atom, an oxygen atom and a sulfur atom. Examples thereof include furyl, thienyl, pyrrolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, imidazolyl, pyrazolyl, oxadiazolyl(1,2,5-oxadiazolyl, 1,3,4-oxadiazolyl, 1,2,4-oxadiazolyl), thiadiazolyl(1,2,5-thiadiazolyl, 1,3,4-thiadiazolyl, 1,2,4-thiadiazolyl), triazolyl(1,2,3-triazolyl, 1,2,4-triazolyl), pyridyl, pyrimidinyl, pyridazinyl, pyrazinyl, 1,3,5-triazinyl and the like. Of these, preferred is pyridyl.
  • The “4-, 5- or 6-membered saturated heterocyclyl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms” means 4-, 5- or 6-membered monocyclic saturated heterocyclyl containing, besides carbon atoms, 1, 2 or 3 hetero atoms selected from a nitrogen atom, an oxygen atom and a sulfur atom. The carbon atom of the heterocycle is optionally substituted by oxo. When a sulfur atom is contained as a hetero atom, the sulfur atom is optionally monooxidized or dioxidized. Examples thereof include oxetanyl, azetidinyl, tetrahydrofuryl, tetrahydropyranyl, tetrahydrothienyl, tetrahydrothiopyranyl, oxazolidinyl, isoxazolidinyl, thiazolidinyl, isothiazolidinyl, imidazolidinyl, pyrazolidinyl, pyrrolidinyl, piperidyl (including piperidino), morpholinyl (including morpholino), thiomorpholinyl (including thiomorpholino), piperazinyl, 1,1-dioxidoisothiazolidinyl, 1,1-dioxidotetrahydrothienyl, 1,1-dioxidotetrahydrothiopyranyl, 1,1-dioxidothiomorpholinyl (including 1,1-dioxidothiomorpholino) and the like. In addition, the saturated heterocyclyl may be partially saturated. Examples thereof include imidazolinyl, oxazolinyl, pyrazolinyl, thiazolinyl and the like. Of these, preferred is oxetanyl.
  • The “C1-6 alkylsulfanyl” means sulfanyl bonded to the above-defined “C1-6 alkyl”. Examples thereof include methylsulfanyl, ethylsulfanyl, propylsulfanyl, isopropylsulfanyl, n-butylsulfanyl, isobutylsulfanyl, sec-butylsulfanyl, tert-butylsulfanyl, pentylsulfanyl, 1,1-dimethylpropylsulfanyl, 2,2-dimethylpropylsulfanyl, hexylsulfanyl and the like.
  • The “C2-6 alkynyl” means straight chain or branched chain hydrocarbon having 2 to 6 carbon atoms and at least one triple bond. Examples thereof include ethynyl, 1-propynyl, 2-propynyl, 1-butynyl, 2-butynyl, 3-butynyl, 1-methyl-2-propynyl, 3,3-dimethylbutynyl (that is, 3,3-dimethylbut-1-ynyl) and the like.
  • The “—(CnH2n)—” means straight chain or branched chain alkylene having n carbon atoms and 2n hydrogen atoms. Examples thereof include —CH2—, —CH2CH2—, —CH(CH3)—, —CH2CH2CH2—, —C(CH3)2—, —CH(CH3)CH2— and the like.
  • When R2 is (10) —(CnH2n)—Rb and Rb is (k) —NRb14C(O)Rb15, “(ii) C1-8 alkyl (said C1-8 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of hydroxy, haloC1-4 alkyl, C1-6 alkoxy and C6-10 aryl)” for Rb15 means the above-defined “C1-8 alkyl” substituted or not substituted by the same or different, 1, 2 or 3 substituents selected from the group consisting of hydroxy, the above-defined “haloC1-4 alkyl”, the above-defined “C1-6 alkoxy” and the above-defined “C6-10 aryl”, at the substitutable position(s) thereof. Examples of Rb include 2-ethoxy-3-methoxypropylcarbonylamino, 1-methyl-1-methoxy-2,2,2-trifluoroethylcarbonylamino and the like.
  • When R2 is (10) —(CnH2n)—Rb and Rb is (k) —NRb14C(O)Rb15, “(iv) C3-7 cycloalkyl (said C3-7 cycloalkyl is optionally substituted by 1, 2, 3 or 4 substituents selected from the group consisting of C1-6 alkyl, halogen, hydroxyC1-6 alkyl and haloC1-4 alkyl, and/or optionally form a fused ring with a benzene ring)” for Rb15 means (1) the above-defined “C3-7 cycloalkyl” substituted by the same or different, 1, 2, 3 or 4 substituents selected from the group consisting of the above-defined “C1-6 alkyl”, the above-defined “halogen”, the above-defined “hydroxyC1-6 alkyl” and the above-defined “haloC1-4 alkyl”, at the substitutable position(s) thereof, (2) unsubstituted C3-7 cycloalkyl, or (3) C3-7 cycloalkyl of (1) or (2), fused with one benzene ring at a fusible position. Examples of Rb include 1,2,3,4-tetrahydro-naphthalen-2-ylcarbonylamino, 2-methyl-indan-2-ylcarbonylamino and the like.
  • When R2 is (10) —(CnH2n)—Rb and Rb is (k) —NRb14C(O)Rb15, “Rb14 and Rb15 optionally form a 4-, 5- or 6-membered lactam together with the nitrogen atom that Rb14 is bonded to and the carbon atom that Rb15 is bonded to” means that Rb is 2-oxo-azetidin-1-yl, 2-oxo-pyrrolidin-1-yl, 2-oxo-piperidin-1-yl or the like.
  • In addition, in this case, “said lactam is optionally substituted by 1, 2 or 3 C1-6 alkyls, and/or optionally form a fused ring with a benzene ring” means that, in addition to the above-mentioned “lactam”, (1) the same or different 1, 2 or 3 C1-6 alkyls defined above are present at the substitutable position(s) of the lactam, (2) one benzene ring is fused at the fusible position of the lactam, and (3) one benzene ring is fused at the fusible position of the lactam substituted by C1-6 alkyl(s). Examples of Rb include 3,4-dimethyl-2-oxo-pyrrolidin-1-yl, 1-oxo-1,3-dihydro-isoindol-2-yl, 3,3-dimethyl-2-oxo-2,3-dihydro-indol-1-yl and the like.
  • In the compound represented by the formula [I], preferable embodiments of respective groups are as described below.
  • R1 is preferably chloro, methyl, cyano or trifluoromethyl, more preferably chloro or trifluoromethyl, and further preferably chloro.
  • R2 is preferably
      • (1) halogen,
      • (2) hydroxy,
      • (3) carboxy,
      • (5) C1-6 alkoxy,
      • (6) haloC1-4 alkoxy,
      • (7) haloC1-4 alkyl,
      • (8) C1-6 alkyl-carbonyl,
      • (9) —C(O)NRa1Ra2 (Ra1 and Ra2 are as defined above) or
      • (10) —(CnH2n)—Rb (Rb is as defined above), more preferably
      • (10) —(CnH2n)—Rb (Rb is as defined above).
      • Rb is preferably
        • (g) —NRb5C(O) NRb6Rb7 (Rb5, Rb6 and Rb7 are as defined above),
        • (h) —NRb8Rb9 (Rb8 and Rb9 are as defined above),
        • (i) —NRb10S(O)2Rb11 (Rb10 and Rb11 are as defined above),
        • (j) —NRb12C(O)ORb13 (Rb12 and Rb13 are as defined above), or
        • (k) —NRb14C(O)Rb15 (Rb14 and Rb15 are as defined above, more preferably
        • (k) —NRb14C(O)Rb15 (Rb14 and Rb15 are as defined above).
      • n is preferably 1 or 2, more preferably 1.
      • Rb14 is preferably hydrogen or methyl, more preferably hydrogen.
      • Rb15 is preferably
        • (ii) C1-4 alkyl (said C1-4 alkyl is optionally substituted by 1 or 2 substituents selected from the group consisting of hydroxy, trifluoromethyl, C1-4 alkoxy and phenyl) or
        • (iv) C3-7 cycloalkyl (said C3-7 cycloalkyl is optionally substituted by 1, 2, 3 or 4 substituents selected from the group consisting of C1-4 alkyl, halogen, hydroxyC1-4 alkyl and trifluoromethyl),
    • more preferably C1-4 alkyl optionally substituted by 1 or 2 trifluoromethyls and C1-4 alkoxy, or C3-7 cycloalkyl optionally substituted by one trifluoromethyl, further preferably tert-butyl, 3,3,3-trifluoro-2,2-dimethylpropyl, 3,3,3-trifluoro-2-methoxy-2-methylpropyl, 3,3,3-trifluoro-2-methyl-2-trifluoromethylpropyl, or 1-trifluoromethylcyclopropyl.
  • R3 is preferably
      • (3) C1-6 alkyl or
      • (4) —ORc {Rc is C1-6 alkyl optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (a) to (f);
        • (a) halogen,
        • (b) hydroxy,
        • (c) C1-6 alkoxy,
        • (d) —C(O)NRc1Rc2 (Rc1 and Rc2 are as defined above),
        • (e) C6-10 aryl (said C6-10 aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
          • (i) halogen,
          • (ii) hydroxy,
          • (iii) C1-6 alkyl,
          • (iv) C1-6 alkoxy, and
          • (v) haloC1-4 alkyl), and
        • (f) 5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms (said heteroaryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
          • (i) halogen,
          • (ii) hydroxy,
          • (iii) C1-6 alkyl,
          • (iv) C1-6 alkoxy, and
          • (v) haloC1-4 alkyl)}.
      • Rc is preferably methyl optionally substituted by 1 or 2 substituents selected from the following (e) and (f);
        • (e) C6-10 aryl (said C6-10 aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
          • (i) halogen,
          • (ii) hydroxy,
          • (iii) C1-6 alkyl,
          • (iv) C1-6 alkoxy, and
          • (v) haloC1-4 alkyl), and
        • (f) 5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms (said heteroaryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
          • (i) halogen,
          • (ii) hydroxy,
          • (iii) C1-6 alkyl,
          • (iv) C1-6 alkoxy, and
          • (v) haloC1-4 alkyl),
            more preferably methyl optionally substituted by 1 or 2 substituents selected from the following (e1) and (f1);
        • (e1) phenyl (said phenyl is optionally substituted by 1 or 2 substituents selected from the group consisting of
          • (i) halogen,
          • (ii) hydroxy,
          • (iii) C1-6 alkyl,
          • (iv) C1-6 alkoxy, and
          • (v) haloC1-4 alkyl), and
        • (f1) pyridyl (said pyridyl is optionally substituted by 1 or 2 substituents selected from the group consisting of
          • (i) halogen,
          • (ii) hydroxy,
          • (iii) C1-6 alkyl,
          • (iv) C1-6 alkoxy, and
          • (v) haloC1-4 alkyl).
  • R4 is preferably hydrogen, fluoro, chloro, or methyl, more preferably hydrogen.
  • R5 is preferably
      • (1) halogen,
      • (4) C1-6 alkyl (said C1-6 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of halogen, C6-10 aryl and C1-6 alkoxy),
      • (5) C3-7 cycloalkyl,
      • (6) —ORd {Rd is
        • (a) C2-6 alkynyl,
        • (b) C3-7 cycloalkyl optionally substituted by 1, 2 or 3 C1-6 alkyls or
        • (c) C1-8 alkyl (said C1-8 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (i) to (v);
          • (i) halogen,
          • (ii) C6-10 aryl,
          • (iii) C1-6 alkoxy,
          • (iv) C3-7 cycloalkyl (said C3-7 cycloalkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C1-6 alkyl and haloC1-4 alkyl), and
          • (v) 4-, 5- or 6-membered saturated heterocyclyl containing one oxygen atom (said saturated heterocyclyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C1-6 alkyl and haloC1-4 alkyl))}, or
      • (7) the formula:
  • Figure US20200087266A1-20200319-C00017
    • wherein Re is
      • (a) C1-6 alkyl,
      • (b) C3-7 cycloalkyl,
      • (c) 5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms, or
      • (d) C6-10 aryl (said C6-10 aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
        • (i) halogen,
        • (ii) C1-6 alkyl,
        • (iii) haloC1-4 alkyl,
        • (iv) C1-6 alkoxy, and
        • (v) haloC1-4 alkoxy).
      • Rd is preferably C1-8 alkyl (said C1-8 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (i) to (v);
        • (i) halogen,
        • (ii) C6-10 aryl,
        • (iii) C1-6 alkoxy,
        • (iv) C3-7 cycloalkyl (said C3-7 cycloalkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C1-6 alkyl and haloC1-4 alkyl), and
        • (v) 4-, 5- or 6-membered saturated heterocyclyl containing one oxygen atom (said saturated heterocyclyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C1-6 alkyl and haloC1-4 alkyl)).
      • Re is preferably
        • (b) C3-7 cycloalkyl,
        • (c) 5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms, or
        • (d) C6-10 aryl (said C6-10 aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
          • (i) halogen,
          • (ii) C1-6 alkyl,
          • (iii) haloC1-4 alkyl,
          • (iv) C1-6 alkoxy, and
          • (v) haloC1-4 alkoxy).
  • m1 is preferably 0, 1 or 2, more preferably 1 or 2.
  • In the compound represented by the formula [I], one of preferable embodiments is a compound represented by the following formula [I-A]:
  • Figure US20200087266A1-20200319-C00018
    • wherein
    • a carbon atom with a hydrogen atom is not substituted by R4 and R5,
    • X, R1, R2 and R4 are as defined in the aforementioned formula [I],
    R5 is
      • (1) halogen,
      • (4) C1-6 alkyl (said C1-6 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of halogen, C6-10 aryl and C1-6 alkoxy),
      • (5) C3-7 cycloalkyl, or
      • (6) —ORd {Rd is
        • (a) C2-6 alkynyl or
        • (c) C1-8 alkyl (said C1-8 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (i) to (v);
          • (i) halogen,
          • (ii) C6-10 aryl,
          • (iii) C1-6 alkoxy,
          • (iv) C3-7 cycloalkyl (said C3-7 cycloalkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C1-6 alkyl and haloC1-4 alkyl), and
          • (v) 4-, 5- or 6-membered saturated heterocyclyl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms (said saturated heterocyclyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C1-6 alkyl and haloC1-4 alkyl))}, and
    • m7 is 0, 1 or 2 and, when m7 is 2, each R5 is selected independently.
  • In a compound represented by the formula [I], one of the preferable other embodiments is a compound represented by the following formula [I-B]:
  • Figure US20200087266A1-20200319-C00019
    • wherein
    • a carbon atom with a hydrogen atom is not substituted by R4 and R5,
    • X, R3 and R4 are as defined in the aforementioned formula [I],
    • R1 is chloro or trifluoromethyl,
    • R5 is
      • (4) C1-6 alkyl (said C1-6 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of halogen, C6-10 aryl and C1-6 alkoxy),
      • (6) —ORd {Rd is
    • C1-8 alkyl (said C1-8 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (i) to (iv);
      • (i) halogen,
      • (ii) C6-10 aryl,
      • (iii) C1-6 alkoxy, and
      • (iv) C3-7 cycloalkyl (said C3-7 cycloalkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C1-6 alkyl and haloC1-4 alkyl))}, or
      • (7) the formula:
  • Figure US20200087266A1-20200319-C00020
    • wherein Re is
      • (b) C3-7 cycloalkyl, or
      • (d) C6-10 aryl (said C6-10 aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
        • (i) halogen,
        • (ii) C1-6 alkyl,
        • (iii) haloC1-4 alkyl,
        • (iv) C1-6 alkoxy, and
        • (v) haloC1-4 alkoxy), and
    • m7 is 0, 1 or 2 and, when m7 is 2, each R5 is selected independently.
  • In a compound represented by the formula [I], one of the preferable other embodiments is a compound represented by the following formula [I-C]:
  • Figure US20200087266A1-20200319-C00021
    • wherein
    • X is CH or N,
    • Rb15 is
      • (ii) C1-4 alkyl (said C1-4 alkyl is optionally substituted by 1 or 2 substituents selected from trifluoromethyl and methoxy) or
      • (iv) C3-7 cycloalkyl optionally substituted by trifluoromethyl,
    • R5a is
      • (1) fluoro,
      • (4) methyl (said methyl is optionally substituted by 3 fluoros), or
      • (6) —ORd {Rd is
        • (a) C2-4 alkynyl or
        • (c) C1-4 alkyl optionally substituted by one C3-7 cycloalkyl (said C3-7 cycloalkyl is optionally substituted by trifluoromethyl)},
    • R5b is
      • (1) halogen,
      • (4) C1-4 alkyl, or
      • (5) cyclopropyl, and
    • m8 is 0 or 1.
  • A pharmaceutically acceptable salt of a compound represented by the formula [I] (hereinafter to be also referred to as the compound of the present invention) may be any salt as long as it forms a nontoxic salt with the compound of the present invention, and examples thereof include salts with inorganic acid, salts with organic acid, salts with inorganic base, salts with organic base, salts with amino acid and the like.
  • Various forms of pharmaceutically acceptable salts are well known in this field and, for example, they are described in the following documents.
    • (a) Berge et al., J. Pharm. Sci., 66, p 1-19 (1977),
    • (b) Stahl et al., “Handbook of Pharmaceutical Salt: Properties, Selection, and Use” (Wiley-VCH, Weinheim, Germany, 2002),
    • (c) Paulekuhn et al., J. Med. Chem., 50, p 6665-6672 (2007)
  • Examples of the salts with inorganic acid include salts with hydrochloric acid, nitric acid, sulfuric acid, phosphoric acid, hydrobromic acid and the like.
  • Examples of the salts with organic acid include salts with oxalic acid, maleic acid, citric acid, fumaric acid, lactic acid, malic acid, succinic acid, tartaric acid, acetic acid, trifluoroacetic acid, gluconic acid, ascorbic acid, methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid and the like.
  • Examples of the salts with organic acid include salts with adipic acid, alginic acid, 4-aminosalicylic acid, anhydromethylenecitric acid, benzoic acid, calcium edetate, camphoric acid, camphor-10-sulfonic acid, carbonic acid, edetic acid, ethane-1,2-disulfonic acid, dodecylsulfuric acid, ethanesulfonic acid, glucoheptonic acid, glucuronic acid, glucoheptonic acid, glycollyarsanilic acid, hexylresorcinic acid, hydrofluoric acid, hydroiodic acid, hydroxy-naphtoic acid, 2-hydroxy-1-ethanesulfonic acid, lactobionic acid, mandelic acid, methylsulfuric acid, methylnitric acid, methylenebis(salicylic acid), galactaric acid, naphthalene-2-sulfonic acid, 2-naphtoic acid, 1,5-naphthalenedisulfonic acid, oleic acid, pamoic acid, pantothenic acid, pectin acid, picric acid, propionic acid, polygalacturonic acid, salicylic acid, stearic acid, tannic acid, teoclic acid, thiocyanic acid, undecanoic acid and the like.
  • Examples of the salts with inorganic base include sodium salt, potassium salt, calcium salt, magnesium salt, ammonium salt and the like.
  • Furthermore, examples of the salts with inorganic base include salts with aluminum, barium, bismuth, lithium, or zinc.
  • Examples of the salts with organic base include salts with methylamine, diethylamine, trimethylamine, triethylamine, ethanolamine, diethanolamine, triethanolamine, ethylenediamine, tris(hydroxymethyl)methylamine, dicyclohexylamine, N,N′-dibenzylethylenediamine, guanidine, pyridine, picoline, choline, cinchonine, meglumine and the like.
  • Furthermore, examples of the salts with organic base include salts with arecoline, betaine, clemizole, N-methylglucamine, N-benzylphenethylamine or tris(hydroxymethyl)methylamine.
  • Examples of the salts with amino acid include salts with lysine, arginine, aspartic acid, glutamic acid and the like.
  • Among the above-mentioned salts, preferred are salts with hydrochloric acid, sulfuric acid or p-toluenesulfonic acid.
  • Various salts can be obtained by reacting a compound represented by the formula [I] with inorganic base, organic base, inorganic acid, organic acid or amino acid according to a known method.
  • A compound represented by the formula [I] or a pharmaceutically acceptable salt thereof may be present as a solvate. The “solvate” is a compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, which is coordinated with a solvent molecule, and also encompasses hydrates. The solvate is preferably a pharmaceutically acceptable solvate, examples thereof include a hydrate, ethanolate, dimethyl sulfoxidate and the like of a compound represented by the formula [I] or a pharmaceutically acceptable salt thereof. Specific examples include semihydrate, monohydrate, dihydrate or monoethanolate of a compound represented by the formula [I], monohydrate of sodium salt or 2/3 ethanolate of dihydrochloride of a compound represented by the formula [I], and the like.
  • The solvates can be obtained by a known method.
  • In addition, a compound represented by the formula [I] may be labeled with isotope (e.g., 2H, 3H, 14C, 35S etc.).
  • The compound of the present invention may exist as a tautomer. In this case, the compound of the present invention can be a single tautomer or a mixture of individual tautomers. For example, a compound represented by the formula [I] may contain a tautomer shown below
  • Figure US20200087266A1-20200319-C00022
  • Such tautomer is also encompassed in the compound represented by the formula [I].
  • The compound of the present invention may have a carbon double bond. In this case, the compound of the present invention can be present as E form, Z form, or a mixture of E form and Z form.
  • The compound of the present invention may contain a stereoisomer that should be recognized as a cis/trans isomer. In this case, the compound of the present invention can be present as a cis form, a trans form, or mixture of a cis form and a trans form.
  • The compound of the present invention may contain one or more asymmetric carbons. In this case, the compound of the present invention may be present as a single enantiomer, a single diastereomer, a mixture of enantiomers or a mixture of diastereomers.
  • The compound of the present invention may be present as an atropisomer. In this case, the compound of the present invention may be present as an individual atropisomer or a mixture of atropisomers.
  • The compound of the present invention may simultaneously contain plural structural characteristics that produce the above-mentioned isomers. Moreover, the compound of the present invention may contain the above-mentioned isomers at any ratio.
  • In the absence of other reference such as annotation and the like, the formulae, chemical structures and compound names indicated in the present specification without specifying the stereochemistry thereof encompass all the above-mentioned isomers that may exist.
  • A diastereomeric mixture can be separated into each diastereomer by conventional methods such as chromatography, crystallization and the like. In addition, each diastereomer can also be formed by using a stereochemically single starting material, or by a synthesis method using a stereoselective reaction.
  • An enantiomeric mixture can be separated into each single enantiomer by a method well known in the pertinent field.
  • For example, enantiomeric mixture can be prepared by reacting the enantiomeric mixture with a substantially pure enantiomer that is known as a chiral auxiliary. The diastereomeric mixture can be separated into each diastereomer mentioned above. The diastereomer mixture can be separated into each diastereomer as mentioned above. The separated diastereomer can be converted to a desired enantiomer by removing the added chiral auxiliary by cleavage.
  • In addition, a mixture of enantiomers of a compound can also be directly separated by a chromatography method using a chiral solid phase well known in the pertinent field.
  • Alternatively, one of the enantiomers of a compound can also be obtained by using a substantially pure optically active starting material or stereoselective synthesis (asymmetric induction) of a prochiral intermediate using a chiral auxiliary and an asymmetric catalyst.
  • The absolute steric configuration can be determined based on the X-ray crystal analysis of the resultant crystalline product or intermediate. In this case, a resultant crystalline product or intermediate derivatized with a reagent having an asymmetric center with a known steric configuration may be used where necessary.
  • As a compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, a substantially purified compound represented by the formula [I] or a pharmaceutically acceptable salt thereof is preferable. More preferred is a compound represented by the formula [I] or a pharmaceutically acceptable salt thereof or a solvate thereof, which is purified to have a purity of more than 80%.
  • Examples of the “pharmaceutical composition” include oral preparations such as tablet, capsule, granule, powder, troche, syrup, emulsion, suspension and the like, and parenteral agents such as external preparation, suppository, injection, eye drop, nasal preparations, pulmonary preparation and the like.
  • The pharmaceutical composition of the present invention is produced according to a method known per se in the art of pharmaceutical preparations, by mixing etc. a compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, or a solvate thereof with a suitable amount of at least one kind of pharmaceutically acceptable carrier and the like as appropriate. While the content of the compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, or a solvate thereof in the pharmaceutical composition varies depending on the dosage form, dose and the like, it is, for example, 0.00001 to 100 wt % of the whole composition.
  • Examples of the “pharmaceutically acceptable carrier” include various organic or inorganic carrier substances conventionally used as preparation materials, for example, excipient, disintegrant, binder, glidant, lubricant and the like for solid preparations, and solvent, solubilizing agent, suspending agent, isotonicity agent, buffering agent, soothing agent, surfactant, pH adjuster, thickening agent and the like for liquid preparations. Where necessary, moreover, additives such as preservative, antioxidant, colorant, sweetening agent and the like are used.
  • Examples of the “excipient” include lactose, sucrose, D-mannitol, D-sorbitol, cornstarch, dextrin, microcrystalline cellulose, crystalline cellulose, carmellose, carmellose calcium, sodium carboxymethyl starch, low-substituted hydroxypropylcellulose, gum arabic and the like.
  • Examples of the “disintegrant” include carmellose, carmellose calcium, carmellose sodium, sodium carboxymethyl starch, croscarmellose sodium, crospovidone, low-substituted hydroxypropylcellulose, hydroxypropylmethylcellulose, crystalline cellulose and the like.
  • Examples of the “binder” include hydroxypropylcellulose, hydroxypropylmethylcellulose, povidone, crystalline cellulose, sucrose, dextrin, starch, gelatin, carmellose sodium, gum arabic and the like.
  • Examples of the “glidant” include light anhydrous silicic acid, magnesium stearate and the like.
  • Examples of the “lubricant” include magnesium stearate, calcium stearate, talc and the like.
  • Examples of the “solvent” include purified water, ethanol, propylene glycol, macrogol, sesame oil, corn oil, olive oil and the like.
  • Examples of the “solubilizing agent” include propylene glycol, D-mannitol, benzyl benzoate, ethanol, triethanolamine, sodium carbonate, sodium citrate and the like.
  • Examples of the “suspending agent” include benzalkonium chloride, carmellose, hydroxypropylcellulose, propylene glycol, povidone, methylcellulose, glycerol monostearate and the like.
  • Examples of the “isotonicity agent” include glucose, D-sorbitol, sodium chloride, D-mannitol and the like.
  • Examples of the “buffering agent” include sodium hydrogenphosphate, sodium acetate, sodium carbonate, sodium citrate and the like.
  • Examples of the “soothing agent” include benzyl alcohol and the like.
  • Examples of the “surfactant” include polyoxyethylene hydrogenated castor oil, polyethylene glycol monostearate, polyoxyethylene sorbitan fatty acid ester, alkyldiaminoethylglycine, alkylbenzenesulfonate, benzethonium chloride and the like.
  • Examples of the “pH adjuster” include hydrochloric acid, sulfuric acid, phosphoric acid, citric acid, acetic acid, sodium hydrogen carbonate, sodium carbonate, potassium hydroxide, sodium hydroxide, monoethanolamine, triethanolamine and the like.
  • Examples of the “thickening agent” include polyvinyl alcohol, carboxyvinyl polymer, methylcellulose, hydroxyethylcellulose, polyethylene glycol, dextran and the like.
  • Examples of the “preservative” include ethyl parahydroxybenzoate, chlorobutanol, benzyl alcohol, sodium dehydroacetate, sorbic acid and the like.
  • Examples of the “antioxidant” include sodium sulfite, ascorbic acid and the like.
  • Examples of the “colorant” include food colors (e.g., Food Color Red No. 2 or 3, Food Color Yellow No. 4 or 5 etc.), β-carotene and the like.
  • Examples of the “sweetening agent” include saccharin sodium, dipotassium glycyrrhizinate, aspartame and the like.
  • The pharmaceutical composition of the present invention can be administered orally or parenterally (e.g., topical, rectal, intravenous administration etc.) to human as well as mammals other than human (e.g., hamster, guinea pig, cat, dog, swine, bovine, horse, sheep, monkey etc.). The dose varies depending on the subject of administration, disease, symptom, dosage form, administration route and the like. For example, the daily dose for oral administration to an adult patient (body weight: about 60 kg) is generally within the range of about 0.1 μg to 10 g, based on the compound of the present invention as the active ingredient. This amount can be administered in one to several portions.
  • The above-mentioned compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, or a solvate thereof can be used in combination with one or a plurality of other medicaments (hereinafter to be also referred to as a concomitant drug) according to a method generally employed in the medical field (hereinafter to be referred to as combined use).
  • The administration period of the above-mentioned compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, and a concomitant drug is not limited, and they may be administered to an administration subject as combination preparation, or the both preparations may be administered simultaneously or at given intervals as individual preparations. In addition, the pharmaceutical composition of the present invention and a concomitant drug may be used in the form of a kit. The dose of the concomitant drug is similar to the clinically-employed dose and can be appropriately selected according to the subject of administration, disease, symptom, dosage form, administration route, administration time, combination and the like. The administration form of the concomitant drug is not particularly limited, and it is only required that the compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, or a solvate thereof is combined with a concomitant drug.
  • Examples of the concomitant drug include therapeutic agents for glaucoma such as prostaglandin preparation, β blocker, α receptor agonist, sympathetic nerve stimulation agent, a blocker, carbonic anhydrase inhibitor, anticholinesterase agent, Rho kinase inhibitor and the like.
  • Examples of the prostaglandin preparation include isopropyl unoprostone, latanoprost, travoprost, tafluprost, bimatoprost and the like.
  • Examples of the β blocker include timolol maleate, Befunolol hydrochloride, carteolol hydrochloride, betaxolol hydrochloride, nipradilol, levobunolol hydrochloride and the like.
  • Examples of the α receptor agonist include brimonidine tartrate and the like.
  • Examples of the sympathetic nerve stimulation agent include dipivefrin hydrochloride, pilocarpine hydrochloride and the like.
  • Examples of the a blocker include bunazosin hydrochloride and the like.
  • Examples of the carbonic anhydrase inhibitor include dorzolamide hydrochloride, brinzolamide and the like.
  • Examples of the anticholinesterase agent include distigmine bromide and the like.
  • Examples of the Rho kinase inhibitor include ripasudil hydrochloride hydrate and the like.
  • An example of the specific combination of medicaments is a combination of one medicament selected from latanoprost, travoprost, tafluprost, timolol maleate, dorzolamide hydrochloride and brinzolamide, and the above-mentioned compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, or a solvate thereof.
  • Next, one example of the production methods of the compound to practice the present invention is explained below. However, the production method of the compound of the present invention or a pharmaceutically acceptable salt thereof is not limited thereto.
  • Even when no directly corresponding disclosure is found in the following Production Methods, the steps may be modified for efficient production of the compound, such as introduction of a protecting group into a functional group with deprotection in a subsequent step, changing the order of Production Methods and steps, appropriate use of reagents other than the exemplified reagents to promote progress of the reactions, and the like.
  • The treatment after reaction in each step may be conventional ones, where isolation and purification can be performed as necessary according to a method appropriately selected from conventional methods such as crystallization, recrystallization, distillation, partitioning, silica gel chromatography, preparative HPLC and the like, or a combination of those methods. In some cases, the next step may be conducted without isolation and purification.
  • An intermediate capable of forming a salt may also be obtained as a salt, or used as a salt for reactions. Examples of such salt include hydrochloride of an intermediate having an amino group.
    • [Production Method 1-1]
  • Figure US20200087266A1-20200319-C00023
  • wherein Hal1 is chloro or bromo;
    • R6 is C1-6 alkyl such as methyl, ethyl and the like or benzyl;
    • Z is a boron substituent used for the Suzuki coupling reaction such as —B(OH)2, —B(OR7)2 (wherein R7 is C1-4 alkyl or one R7 may be bonded to the other R7 to form a ring), —BF3, the formula
  • Figure US20200087266A1-20200319-C00024
  • and the like; and
    • X, Cy, R5 and m1 are as defined in the aforementioned formula [I].
    (Step 1-1-1)
  • Compound [3] can be obtained by the Suzuki coupling reaction of compound [1] and compound [2]. For example, compound [3] can be obtained by reacting compound [1] with compound [2] under heating in a solvent in the presence of a base and a palladium catalyst. Where necessary, a ligand may be added. Not less than 1.5 equivalents of compound [1] are preferably used relative to compound [2] to prevent the Suzuki coupling reaction from progressing twice.
  • Examples of the palladium catalyst to be used for the reaction include palladium acetate, tetrakistriphenylphosphinepalladium, bis(triphenylphosphine)palladium dichloride, (bis(diphenylphosphino)ferrocene)palladium dichloride-methylene chloride complex and the like.
  • Examples of the base to be used for the reaction include inorganic bases such as alkali metal salts (e.g., potassium phosphate, sodium carbonate, sodium hydrogen carbonate, potassium carbonate, potassium acetate, sodium acetate, cesium fluoride etc.) and the like, and organic bases such as triethylamine and the like.
  • Examples of the ligand to be used for the reaction include organic phosphine ligands (e.g., triphenylphosphine, tricyclohexylphosphine, 2,2′-bis(diphenylphosphino)-1,1′-binaphthalene, 2-dicyclohexylphosphino-2′,6′-dimethoxybiphenyl etc.) and the like.
  • Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like; hydrocarbon solvents such as toluene, xylene, hexane and the like; polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile and the like; a mixed solvent thereof, and a mixed solvent thereof with water.
  • Compound [1] may be a commercially available product such as 2,4-dichloro-6-methoxy-1,3,5-triazine, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • As for the Suzuki coupling reaction, for example, the following review article is known (SUZUKI, A et al. Palladium-Catalyzed Cross-Coupling Reactions of Organoboron Compounds. Chem Rev. 1995, Vol. 95, pages 2457-2483).
    • (Step 1-1-2)
  • Compound [5] can be obtained by the Suzuki coupling reaction of compound [3] and compound [4]. For example, compound [5] can be obtained by reacting compound [3] with compound [4] under heating in a solvent in the presence of a base and a palladium catalyst. Where necessary, a ligand may be added.
  • Examples of the palladium catalyst to be used for the reaction include palladium acetate, tetrakistriphenylphosphinepalladium, bis(triphenylphosphine)palladium dichloride, (bis(diphenylphosphino)ferrocene)palladium dichloride-methylene chloride complex and the like.
  • Examples of the base to be used for the reaction include inorganic bases such as alkali metal salts (e.g., potassium phosphate, sodium carbonate, sodium hydrogen carbonate, potassium carbonate, potassium acetate, sodium acetate, cesium fluoride etc.) and the like, and organic bases such as triethylamine and the like.
  • Examples of the ligand to be used for the reaction include organic phosphine ligands such as triphenylphosphine, tricyclohexylphosphine, 2,2′-bis(diphenylphosphino)-1,1′-binaphthalene, 2-dicyclohexylphosphino-2′,6′-dimethoxybiphenyl and the like, and the like.
  • Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like; hydrocarbon solvents such as toluene, xylene, hexane and the like; polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile and the like; a mixed solvent thereof, and a mixed solvent thereof with water.
    • (Step 1-1-3)
  • Compound [I] can be obtained by converting the alkoxy of compound [5] to hydroxy by hydrolysis. For example, when R6 is C1-6 alkyl, compound [I] can be obtained by reacting compound [5] in a solvent in the presence of a base at room temperature to under heating, and neutralizing the obtained solution.
  • Examples of the base to be used for the reaction include lithium hydroxide, sodium hydroxide, potassium hydroxide, sodium methoxide and the like.
  • Examples of the solvent to be used for the reaction include a mixed solvent of water and alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like; and a mixed solvent thereof with ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like.
    • [Production Method 1-2]
  • Compound [2] can be obtained by, for example, Production Method 1-2.
    • [Production Method 1-2]
  • Figure US20200087266A1-20200319-C00025
  • wherein L1 is a leaving group such as bromo, iodo, trifluoromethanesulfonyloxy and the like, X, R5 and m1 are as defined in the aforementioned formula [I], and Z is as defined in the aforementioned Production Method 1-1.
    • (Step 1-2)
  • Compound [2] can be obtained by borating compound [6]. For example, compound [2] can be obtained by reacting compound [6] with a boron reagent under heating in the presence of a base and a palladium catalyst. Where necessary, a ligand may be added
  • Examples of the boron reagent to be used for the reaction include 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi-1,3,2-dioxaborolane, 5,5,5′,5′-tetramethyl-2,2′-bi-1,3,2-dioxaborinane, tetrahydroxydiboron, 4,4,5,5-tetramethyl-1,3,2-dioxaborolane and the like.
  • Examples of the palladium catalyst to be used for the reaction include palladium acetate, tetrakistriphenylphosphinepalladium, bis(triphenylphosphine)palladium dichloride, (bis(diphenylphosphino)ferrocene)palladium dichloride-methylene chloride complex and the like.
  • Examples of the base to be used for the reaction include inorganic bases such as alkali metal salts (e.g., potassium phosphate, sodium carbonate, sodium hydrogen carbonate, potassium carbonate, potassium acetate, sodium acetate, cesium fluoride etc.) and the like, and organic bases such as triethylamine and the like.
  • Examples of the ligand to be used for the reaction include organic phosphorus ligands (e.g., triphenylphosphine, tricyclohexylphosphine, 2,2′-bis(diphenylphosphino)-1,1′-binaphthalene, 2-dicyclohexylphosphino-2′,6′-dimethoxybiphenyl etc.) and the like.
  • Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like; hydrocarbon solvents such as toluene, xylene, hexane and the like; polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile and the like; a mixed solvent thereof, and a mixed solvent thereof with water.
  • Compound [2] can also be obtained by adding an organic metal reagent to compound [6] in a solvent at −78° C. to room temperature, and reacting the product with a boron compound at −78° C. to room temperature.
  • Examples of the organic metal reagent to be used for the reaction include n-butyllithium, tert-butyllithium, isopropylmagnesium chloride and the like.
  • Examples of the boron reagent to be used for the reaction include trimethyl borate, triisopropyl borate, 2-isopropoxy-4,4,5,5-tetramethyl-1,3,2-dioxaborolane and the like.
  • Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, xylene, hexane and the like, and a mixed solvent thereof.
  • In one embodiment, compound [6] may be a commercially available product such as those shown below, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Figure US20200087266A1-20200319-C00026
  • In one embodiment, compound [2] may be a commercially available product such as those shown below, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Figure US20200087266A1-20200319-C00027
    Figure US20200087266A1-20200319-C00028
    Figure US20200087266A1-20200319-C00029
    Figure US20200087266A1-20200319-C00030
  • wherein Z is as defined in the aforementioned Production Method 1-1.
    • [Production Method 1-3]
  • Compound [4] can be obtained by, for example, Production Method 1-3.
    • [Production Method 1-3]
  • Figure US20200087266A1-20200319-C00031
  • wherein R1, R2, R3 and R4 are as defined in the aforementioned formula [I], L1 is as defined in the aforementioned Production Method 1-2, and Z is as defined in the aforementioned Production Method 1-1.
    • (Step 1-3)
  • Compound [4] is compound [8a] or [8b]. Compound [8a] or [8b], i.e., compound [4], can be obtained by borating compound [7a] or [7b] in the same manner as in Production Method 1-2, Step 1-2.
  • Compounds [7a] and [7b] may be commercially available products such as 2-bromo-4-methylbenzonitrile and 2-bromo-3-methylphenol, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • In one embodiment, compound [4] may be a commercially available product such as those shown below, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Figure US20200087266A1-20200319-C00032
  • wherein Z is as defined in the aforementioned Production Method 1-1.
    • [Production Method 2-1] or [Production Method 2-3]
  • For example, compound [I-al] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00033
  • wherein R1, R4, Rb15 and n are as defined in the aforementioned formula [I], can be obtained by appropriately converting the substituent of ring Cy.
  • When CnH2n is a straight chain, Production Method 2-1 is preferable, and when CnH2n is a branched chain, Production Method 2-3 is preferable.
    • [Production Method 2-1]
  • Figure US20200087266A1-20200319-C00034
    Figure US20200087266A1-20200319-C00035
  • wherein Y is the formula
  • Figure US20200087266A1-20200319-C00036
  • wherein R5, R6 and m1 are as defined in the aforementioned formula [I];
    • C1-6 Alkyl is C1-6 alkyl;
    • t is 0, 1, 2 or 3, —(CtH2t)— may be a straight or branched chain;
    • Hal2 is bromo or iodo;
    • Pv is a hydroxy-protecting group such as methoxymethyl and the like;
    • Pw is an amino-protecting group such as tert-butoxycarbonyl and the like;
    • L2 is a leaving group such as halogen (e.g., chloro, bromo and the like), methanesulfonyloxy, p-toluenesulfonyloxy and the like;
    • R1, R4, R6, Rb15 and n are as defined in the aforementioned formula [I], and Z is as defined in the aforementioned Production Method 1-1.
    (Step 2-1-1)
  • Compound [10] can be obtained by converting the ester of compound [9] to carboxy by hydrolysis. For example, compound [10] can be obtained by reacting compound [9] in a solvent in the presence of a base at room temperature to under heating, and neutralizing the obtained solution.
  • Examples of the base to be used for the reaction include lithium hydroxide, sodium hydroxide, potassium hydroxide, sodium methoxide and the like.
  • Examples of the solvent to be used for the reaction include a mixed solvent of water and alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like; and a mixed solvent thereof with ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like.
  • Compound [9] may be a commercially available product such as those shown below, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Figure US20200087266A1-20200319-C00037
    • (Step 2-1-2)
  • Compound [11] can be obtained by converting the carboxy of compound [10] to hydroxy by reduction. For example, compound [11] can be obtained by reacting compound [10] with a reducing agent in a solvent under ice-cooling to room temperature.
  • Examples of the reducing agent to be used for the reaction include lithium aluminum hydride, diisobutylaluminum hydride, sodium bis(2-methoxyethoxy)aluminum hydride, borane-tetrahydrofuran complex and the like.
  • Examples of the solvent to be used for the reaction include tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether, toluene, xylene, hexane and the like and a mixed solvent thereof.
    • (Step 2-1-3)
  • Compound [12] can be obtained by protecting the hydroxy group of compound [11]. The protection reaction can be performed by a known method according to the protecting group to be employed.
  • For example, when Pv is methoxymethyl, compound [12] can be obtained by reacting compound [11] with chloromethyl methyl ether in a solvent such as tetrahydrofuran, 1,2-dimethoxyethane, cyclopentyl methyl ether, N,N-dimethylformamide and the like in the presence of a base such as sodium hydride and the like from ice-cooling to room temperature.
    • (Step 2-1-4)
  • Compound [13] can be obtained by borating compound [12] in the same manner as in Production Method 1-2, Step 1-2.
    • (Step 2-1-5)
  • Compound [14] can be obtained by the Suzuki coupling reaction of compound [3] and compound [13] in the same manner as in Production Method 1-1, Step 1-1-2.
    • (Step 2-1-6)
  • Compound [15] can be obtained by removing Pv of compound [14] by hydroxy-deprotection by a conventional method. The deprotection reaction can be performed by a known method according to the protecting group to be employed.
  • For example, when Pv is methoxymethyl, a treatment with an acid such as hydrochloric acid, trifluoroacetic acid, methanesulfonic acid and the like only needs to be performed in a single or mixed solvent of chloroform, 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether, ethyl acetate, ethanol, methanol, water and the like.
  • Compound [15] can also be obtained by the Suzuki coupling reaction of compound [3] and compound [23] represented by the formula
  • Figure US20200087266A1-20200319-C00038
  • wherein R1 and R4 are as defined in the aforementioned formula [I], Z is as defined in the aforementioned Production Method 1-1, and t is as defined in the aforementioned Production Method 2-1,
    in the same manner as in Production Method 1-1, Step 1-1-2.
    • (Step 2-1-7)
  • Compound [16] can be obtained by converting the hydroxy of compound [15] to the leaving group L2. For example, when L2 is methanesulfonyloxy, compound [16] can be obtained by reacting compound [15] with methanesulfonyl chloride in a solvent in the presence of a base at room temperature. When L2 is bromo, compound [16] can be obtained by reacting compound [15] with carbon tetrabromide in a solvent in the presence of triphenylphosphine from ice-cooling to room temperature.
  • Examples of the base to be used for the reaction include triethylamine, pyridine and the like.
  • Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like; halogenated solvents such as dichloromethane, chloroform and the like; and polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile and the like.
  • It is possible to use dimethylsulfide instead of the above-mentioned triphenylphosphine, and N-bromosuccinimide can be used instead of the above-mentioned carbon tetrabromide.
  • p-Toluenesulfonyl chloride and benzenesulfonyl chloride can be used instead of the above-mentioned methanesulfonyl chloride.
    • (Step 2-1-8)
  • Compound [18] can be obtained by reacting compound [16] in a solvent in the presence of a base at room temperature to under heating compound [17]. Examples of the protecting group Pw include tert-butoxycarbonyl.
  • Examples of the base to be used for the reaction include inorganic bases such as alkali metal salts (e.g., cesium carbonate, potassium phosphate, sodium carbonate, potassium carbonate etc.) and the like.
  • Examples of the solvent to be used for the reaction include polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile and the like.
    • (Step 2-1-9)
  • Compound [19] can be obtained by removing Pw of compound [18] by amine-deprotection by a conventional method. The deprotection reaction can be performed by a known method according to the protecting group to be employed.
  • For example, when Pw is tert-butoxycarbonyl, a treatment with an acid such as hydrochloric acid, trifluoroacetic acid, methanesulfonic acid and the like only needs to be performed in a solvent.
  • Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like; halogenated solvents such as dichloromethane, chloroform and the like; ester solvents such as ethyl acetate and the like; and alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like.
    • (Step 2-1-10)
  • Compound [21] can be obtained by a conventional amide bond forming reaction, for example, by reacting compound [19] with compound [20] in a solvent in the presence of a condensing agent and an additive. A base may be added as necessary.
  • Examples of the condensing agent to be used for the reaction include dicyclohexylcarbodiimide (DCC), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (WSC.HCl), diisopropylcarbodiimide, 1,1′-carbonyldiimidazole (CDI), O-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HATU), (benzotriazol-1-yloxy)tris(pyrrolidino)phosphonium hexafluorophosphate (PyBOP), diphenylphosphoryl azide and the like.
  • Examples of the additive to be used for the reaction include 1-hydroxybenzotriazole (HOBt), 1-hydroxy-7-azabenzotriazole (HOAt), N-hydroxysuccinimide (HOSu), 4-dimethylaminopyridine and the like.
  • Examples of the base to be used for the reaction include organic bases such as pyridine, triethylamine and the like.
  • Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like; halogenated solvents such as dichloromethane, chloroform and the like; and polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile, pyridine and the like. These may be used singly or as a mixture of two or more kinds thereof.
  • Compound [20] may be a commercially available product such as cyclopentanecarboxylic acid and 1-(trifluoromethyl)cyclopropane-1-carboxylic acid, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
    • (Step 2-1-11)
  • Compound [21] can be indicated as compound [22]. Compound [I-a1] can be obtained by converting the alkoxy of compound [22] to hydroxy by hydrolysis in the same manner as in Production Method 1-1, Step 1-1-3.
    • [Production Method 2-2]
  • Compound [10a] which is compound [10] wherein R1 is C1-6 alkyl or chloro can be obtained by [Production Method 2-2].
    • [Production Method 2-2]
  • Figure US20200087266A1-20200319-C00039
  • wherein Rx is C1-6 alkyl or chloro;
    • R4 is as defined in the aforementioned formula [I], and Hal2 and t are as defined in the aforementioned Production Method 2-1.
    (Step 2-2)
  • Compound [10a] can be obtained by halogenating compound [24]. For example, when Hal2 is iodo, compound [10a] can be obtained by reacting compound [24] with N-iodosuccinimide in an acid at room temperature.
  • Examples of the acid to be used for the reaction include concentrated sulfuric acid and the like.
  • Compound [24] may be a commercially available product such as 4-chlorophenylacetic acid, 3-(4-chlorophenyl)propionic acid, 4-(4-chlorophenyl)butanoic acid, 4-methylphenylacetic acid and 2-(4-methylphenyl)propionic acid, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
    • [Production Method 2-3]
  • Figure US20200087266A1-20200319-C00040
    Figure US20200087266A1-20200319-C00041
  • wherein j and k are each 0, 1, 2 or 3, j+k=n−1;
    • R1, R4, R5, Rb15 and n are as defined in the aforementioned formula [I],
    • Z is as defined in the aforementioned Production Method 1-1, and
    • Hale, Y, Pw and L2 are as defined in the aforementioned Production Method 2-1.
    (Step 2-3-1)
  • Compound [26] can be obtained by borating compound [25] in the same manner as in Production Method 1-2, Step 1-2.
  • Compound [25] may be a commercially available product such as 1-(3-bromo-4-chlorophenyl)propan-1-one and 1-(3-bromo-4-chlorophenyl)butan-1-one, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
    • (Step 2-3-2)
  • Compound [27] can be obtained by the Suzuki coupling reaction of compound [3] and compound [26] in the same manner as in Production Method 1-1, Step 1-1-2.
    • (Step 2-3-3)
  • Compound [28] can be obtained by converting the carboxy of compound [27] to hydroxy by reduction. For example, compound [28] can be obtained by reacting compound [27] with a reducing agent in a solvent under ice-cooling to room temperature.
  • Examples of the reducing agent to be used for the reaction include sodium borohydride and the like.
  • Examples of the solvent to be used for the reaction include methanol, ethanol, 2-propanol, 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like.
    • (Step 2-3-4)
  • Compound [29] can be obtained by converting the hydroxy of compound [28] to the leaving group L2 in the same manner as in Production Method 2-1, Step 2-1-7.
    • (Step 2-3-5)
  • Compound [30] can be obtained by reacting compound [29] with compound [17] in the same manner as in Production Method 2-1, Step 2-1-8.
    • (Step 2-3-6)
  • Compound [31] can be obtained by removing Pw of compound [30] in the same manner as in Production Method 2-1, Step 2-1-9.
    • (Step 2-3-7)
  • Compound [32] can be obtained by reacting compound [31] with compound [20] in the same manner as in Production Method 2-1, Step 2-1-10.
    • (Step 2-3-8)
  • Compound [32] can be indicated as compound [22]. Compound [I-al] can be obtained by converting alkoxy of compound [22] to hydroxy by hydrolysis in the same manner as in Production Method 1-1, Step 1-1-3.
  • In Production Method 2-1, compound [I-a2] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00042
  • wherein R1, R4 and n are as defined in the aforementioned formula [I], can be obtained by subjecting compound [9] to the reactions of Step 2-1-4, Step 2-1-5 and Step 2-1-11.
  • In Production Method 2-1, the amide bond forming reaction is performed by using compound [10] and HNRb1Rb2 such as dimethylamine, tert-butylamine and the like and in the same manner as in Step 2-1-10. Thereafter, the resultant product is subjected to the reactions of Step 2-1-4, Step 2-1-5 and Step 2-1-11, whereby compound [I-a3] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00043
  • wherein R1, R4, Rb1, Rb2 and n are as defined in the aforementioned formula [I], can be obtained.
  • In Production Method 2-1, compound [I-a4] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00044
  • wherein R1, R4 and n are as defined in the aforementioned formula [I] can be obtained by subjecting compound [15] to the reaction of Step 2-1-11.
  • In Production Method 2-1, the reaction of Step 2-1-11 is performed by using compound [15]. Thereafter, the resultant product is reacted with a C1-6 alkyl-carboxylic anhydride such as acetic anhydride, propionic anhydride and the like, whereby compound [I-a5] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00045
  • wherein R1, R4 and n are as defined in the aforementioned formula [I] and C1-6 Alkyl is as defined in the aforementioned Production Method 2-1, can be obtained.
  • In Production Method 2-1, compound [15] is reacted with ClC(O)NRb3Rb4 such as dimethylcarbamoyl chloride, diethylcarbamoyl chloride and the like in the presence of a base. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a6] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00046
  • wherein R1, R4, Rb3, Rb4 and n are as defined in the aforementioned formula [I], can be obtained.
  • In Production Method 2-1, compound [15] is subjected to an alkylation reaction by using sodium hydride and a C1-6 alkyl halide. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a7] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00047
  • wherein R1, R4 and n are as defined in the aforementioned formula [I] and C1-6 Alkyl is as defined in the aforementioned Production Method 2-1, can be obtained.
  • In Production Method 2-1, compound [16] is subjected to an amination reaction by using HNRb8Rb9 such as dimethylamine, diethylamine and the like. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a8] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00048
  • wherein R1, R4, Rb8, Rb9 and n are as defined in the aforementioned formula [I], can be obtained.
  • In Production Method 2-1, compound [16] is reacted by using sodium hydride, and HNRb14C(O)Rb15 such as N-methylacetamide, 2-pyrrolidinone and the like. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a9] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00049
  • wherein R1, R4, Rb14, Rb15 and n are as defined in the aforementioned formula [I], can be obtained.
  • In Production Method 2-1, compound [16] is reacted by using sodium hydride and compound [33] represented by the formula
  • Figure US20200087266A1-20200319-C00050
  • wherein Rb17, m5 and m6 are as defined in the aforementioned formula [I]. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a10] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00051
  • wherein R1, R4, Rb17, n, m5 and m6 are as defined in the aforementioned formula [I], can be obtained.
  • In Production Method 2-1, compound [19] is reacted with ClC(O)NRb6Rb7 such as dimethylcarbamoyl chloride, diethylcarbamoyl chloride and the like in the presence of a base. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a11] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00052
  • wherein R1, R4, Rb6, Rb7 and n are as defined in the aforementioned formula [I], can be obtained.
  • In Production Method 2-1, compound [19] is reacted with Rb11S(O)2Cl such as methanesulfonyl chloride and the like in the presence of a base. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a12] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00053
  • wherein R1, R4, Rb11 and n are as defined in the aforementioned formula [I], can be obtained.
  • In Production Method 2-1, compound [19] is reacted with Rb13OC(O)Cl such as ethyl chloroformate and the like in the presence of a base. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a13] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00054
  • wherein R1, R4, Rb13 and n are as defined in the aforementioned formula [I], can be obtained.
  • In Production Method 2-3, compound [I-a14] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00055
  • wherein R1 and R4 are as defined in the aforementioned formula [I], j and k are as defined in the aforementioned Production Method 2-2, can be obtained by subjecting compound [27] to the reaction of Step 2-3-8.
  • By Production Method 2-4, compound [I-a15] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00056
  • wherein R1, R4, Rb16, m2, m3 and m4 are as defined in the aforementioned Production Method 2-1, can be obtained.
    • [Production Method 2-4]
  • Figure US20200087266A1-20200319-C00057
  • wherein R1, R4, Rb16, m2, m3 and m4 are as defined in the aforementioned formula [I] and C1-6 Alkyl, L2, Pv, t and Y are as defined in the above-mentioned Production Method 2-1.
    • (Step 2-4-1)
  • Compound [36] can be obtained by reacting compound [34] with compound [35] in a solvent in the presence of a base.
  • Examples of the base to be used for the reaction include, lithium diisopropylamide, lithium bis(trimethylsilyl)amide and the like base.
  • Examples of the solvent to be used for the reaction include ether solvents such as tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like, and a mixed solvent thereof.
  • Compound [35] may be a commercially available product such as benzyl chloromethyl ether, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
    • (Step 2-4-2)
  • Compound [37] can be obtained by removing Pv of compound [36] in the same manner as in Production Method 2-1, Step 2-1-6.
    • (Step 2-4-3)
  • Compound [38] can be obtained by converting the ester of compound [37] to carboxy by hydrolysis in the same manner as in Production Method 2-1, Step 2-1-1.
    • (Step 2-4-4)
  • Compound [39] can be obtained by reacting compound [38] with compound [19] in a solvent in the presence of a condensing agent and an additive in the same manner as in Production Method 2-1, Step 2-1-10.
    • (Step 2-4-5)
  • Compound [40] can be obtained by cyclization of compound [39] by intramolecular Mitsunobu reaction. For example, compound [40] can be obtained by reacting compound [39] with an azodicarboxylic acid diester (e.g., diethyl azodicarboxylate, diisopropyl azodicarboxylate, bis(2-methoxyethyl) azodicarboxylate etc.) in a solvent in the presence of a phosphine such as triphenylphosphine, tributylphosphine and the like.
  • Examples of the solvent to be used for the reaction include dichloromethane, chloroform, 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether, toluene, N,N-dimethylformamide and the like. These may be used singly or as a mixture of two or more kinds thereof.
    • [Production Method 3-1]
  • Another method of appropriately converting the substituent of ring Cy is, for example, Production Method 3-1 for obtaining compound [I-b1] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00058
  • wherein R1, R4 and Rc are as defined in the aforementioned formula [I].
    • [Production Method 3-1]
  • Figure US20200087266A1-20200319-C00059
  • wherein R1, R4, R5, Rc, m1 and X are as defined in the aforementioned formula [I], Z is as defined in the above-mentioned Production Method 1-1, and Hale and Pv are as defined in the above-mentioned Production Method 2-1.
    • (Step 3-1-1)
  • Compound [42] can be obtained by protecting the hydroxy group of compound [41] in the same manner as in Production Method 2-1, Step 2-1-3.
  • Compound [41] may be a commercially available product such as 2-bromo-3-methylphenol, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
    • (Step 3-1-2)
  • Compound [43] can be obtained by borating compound [42] in the same manner as in Production Method 1-3, Step 1-3.
    • (Step 3-1-3)
  • Compound [44] can be obtained by the Suzuki coupling reaction of compound [3] and compound [43] in the same manner as in Production Method 1-1, Step 1-1-2.
    • (Step 3-1-4)
  • Compound [45] can be obtained by removing Pv of compound [44] in the same manner as in Production Method 2-1, Step 2-1-6.
    • (Step 3-1-5)
  • Compound [47] can be obtained by the Mitsunobu reaction of compound [45] and compound [46]. For example, compound [47] can be obtained by reacting compound [45] with compound [46] in a solvent in the presence of an azodicarboxylic acid diester (e.g., diethyl azodicarboxylate, diisopropyl azodicarboxylate, bis(2-methoxyethyl) azodicarboxylate etc.) and a phosphine such as triphenylphosphine, tributylphosphine and the like.
  • Examples of the solvent to be used for the reaction include dichloromethane, chloroform, 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether, toluene, N,N-dimethylformamide and the like. These may be used singly or as a mixture of two or more kinds thereof.
  • Compound [46] may be a commercially available product such as benzyl alcohol, 2-pyridinemethanol and the like, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
    • (Step 3-1-6)
  • Compound [I-b1] can be obtained by converting the alkoxy of compound [47] to hydroxy by hydrolysis in the same manner as in Production Method 1-1, Step 1-1-3.
  • In Production Method 3-1, for example, compound [I-b2] which is a compound represented by the formula [I] wherein ring Cy is the formula
  • Figure US20200087266A1-20200319-C00060
  • wherein R1 and R4 are as defined in the aforementioned formula [I], can be obtained by subjecting compound [45] to the reaction of Step 3-1-6.
    • [Production Method 3-2]
  • Compound [43a] which is compound [43] wherein R1 is chloro or trifluoromethyl can also be obtained by [Production Method 3-2].
    • [Production Method 3-2]
  • Figure US20200087266A1-20200319-C00061
  • wherein Ry is chloro or trifluoromethyl;
    • R4 is as defined in the aforementioned formula [I], Z is as defined in the aforementioned Production Method 1-1, and Pv is as defined in the aforementioned Production Method 2-1.
    (Step 3-2-1)
  • Compound [49] can be obtained by protecting the hydroxy group of compound [48] in the same manner as in Production
    • Method 2-1, Step 2-1-3.
  • In one embodiment, compound [48] may be a commercially available product such as those shown below, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • Figure US20200087266A1-20200319-C00062
    • (Step 3-2-2)
  • Compound [43a] can be obtained by reacting compound [49] with a boron compound in a solvent in the presence of a base. For example, compound [43a] can be obtained by adding a base to compound [49] in a solvent at −78° C. to room temperature, and reacting the resultant product with a boron reagent at −78° C. to room temperature.
  • Examples of the base to be used for the reaction include n-butyllithium, sec-butyllithium and the like.
  • Examples of the boron reagent to be used for the reaction include 2-isopropoxy-4,4,5,5-tetramethyl-1,3,2-dioxaborolane, trimethyl borate and the like.
  • Examples of the solvent to be used for the reaction include tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like.
    • [Production Method 4]
  • For example, compound [I-c1] of the formula
  • Figure US20200087266A1-20200319-C00063
  • wherein R5, Rc, X and Cy are as defined in the aforementioned formula [I], m7 is 0, 1 or 2, and when m7 is 2, each R5 is selected independently, can be obtained by appropriately converting the substituent of compound [2].
    • [Production Method 4]
  • Figure US20200087266A1-20200319-C00064
    Figure US20200087266A1-20200319-C00065
  • wherein L3 is a leaving group such as trifluoromethanesulfonyloxy and the like;
    • Px is a hydroxy-protecting group such as benzyl and the like;
    • R5, R6, Re, X and Cy are as defined in the aforementioned formula [I], Hal1 and Z are as defined in the aforementioned Production Method 1-1, and m7 is as defined in the aforementioned formula [I-A].
    (Step 4-1)
  • Compound [51] can be obtained by the Suzuki coupling reaction of compound [1] and compound [50] in the same manner as in Production Method 1-1, Step 1-1-1.
  • Compound [50] may be a commercially available product such as 4-(benzyloxy)phenylboronic acid, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
    • (Step 4-2)
  • Compound [52] can be obtained by the Suzuki coupling reaction of compound [4] and compound [51] in the same manner as in Production Method 1-1, Step 1-1-2.
    • (Step 4-3)
  • Compound [53] can be obtained by removing the phenol protecting group Px of compound [52]. The deprotection can be performed by a known method according to the protecting group to be employed.
  • For example, when Px is benzyl, compound [52] only needs to be subjected to a hydrogenation reaction in a single or mixed solvent of tetrahydrofuran, ethyl acetate, ethanol, methanol, water and the like in the presence of a catalyst such as palladium carbon, platinum carbon and the like.
    • (Step 4-4)
  • Compound [54] can be obtained by converting the hydroxy to a leaving group L3. For example, when the leaving group is trifluoromethanesulfonyloxy, compound [54] can be obtained by reacting compound [53] with trifluoromethanesulfonic anhydride, N-phenyl bis(trifluoromethanesulfonimide) and the like in a solvent in the presence of a base from ice-cooling to room temperature.
  • Examples of the base to be used for the reaction include organic bases such as pyridine, 2,6-lutidine, triethylamine and the like; inorganic bases such as alkali metal salts (e.g., cesium carbonate, sodium hydride etc.) and the like.
  • Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like; halogenated solvents such as dichloromethane, chloroform and the like; polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile, pyridine and the like, and the like. These may be used singly or as a mixture of two or more kinds thereof.
    • (Step 4-5)
  • Compound [56] can be obtained by the Sonogashira reaction of compound [54] and compound [55]. For example, compound [56] can be obtained by reacting compound [54] with compound [55] in a solvent preferably under heating in the presence of a base, a palladium catalyst and a copper catalyst.
  • Examples of the palladium catalyst to be used for the reaction include palladium acetate, tetrakistriphenylphosphinepalladium, bis(triphenylphosphine)palladium dichloride, (bis(diphenylphosphino)ferrocene)palladium dichloride-methylene chloride complex and the like.
  • Examples of the copper catalyst to be used for the reaction include copper iodide, copper bromide and the like.
  • Examples of the base to be used for the reaction include diethylamine, dicyclohexylamine, triethylamine, N-ethyldiisopropylamine and the like.
  • Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like; and polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile, pyridine and the like. These may be used singly or as a mixture of two or more kinds thereof.
  • Compound [55] may be a commercially available product such as cyclohexylacetylene, 2-ethynylpyridine and the like, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.
  • As for the Sonogashira coupling reaction, for example, the following review article is known (NAJERA, C et al. The Sonogashira Reaction: A Booming Methodology in Synthetic Organic Chemistry. Chem Rev. 2007, Vol. 107, pages 874-922.).
    • (Step 4-6)
  • Compound [I-c1] can be obtained by converting the alkoxy of compound [56] to hydroxy by hydrolysis in the same manner as in Production Method 1-1, Step 1-1-3.
  • In Production Method 4, compound [I-c2] of the formula
  • Figure US20200087266A1-20200319-C00066
  • wherein R5, X and Cy are as defined in the aforementioned formula [I], and m7 is as defined in the aforementioned formula [I-A], can be obtained by subjecting compound [53] to the reaction of Step 4-6.
  • In Production Method 4, compound [I-c3] of the formula
  • Figure US20200087266A1-20200319-C00067
  • wherein R5, Rd, X and Cy are as defined in the aforementioned formula [I], and m7 is as defined in the aforementioned formula [I-A], can be obtained by the Mitsunobu reaction of compound [53] and RdOH such as cyclohexylmethanol and the like in the same manner as in Production Method 3-1, Step 3-1-5, and subjecting the resultant product to the reaction of Step 4-6.
  • In Production Method 4, the Suzuki coupling reaction of compound [54] and compound [57] of the formula
  • Figure US20200087266A1-20200319-C00068
  • wherein Z is as defined in the aforementioned Production Method 1-1, is performed in the same manner as in Production Method 1-1, Step 1-1-2. The resultant product is subjected to the reaction of Step 4-6, whereby compound [I-c4] of the formula
  • Figure US20200087266A1-20200319-C00069
  • wherein R5, X and Cy are as defined in the aforementioned formula [I] and m7 is as defined in the aforementioned formula [I-A], can be obtained.
  • In Production Method 4, the Suzuki coupling reaction of compound [54] and compound [58] of the formula
  • Figure US20200087266A1-20200319-C00070
  • wherein m9 is 1, 2, 3, or 4, and Z is as defined in the aforementioned Production Method 1-1, is performed in the same manner as in Production Method 1-1, Step 1-1-2. After reduction of the olefin of the resultant product, the obtained product is subjected to the reaction of Step 4-6, whereby compound [I-c5] of the formula
  • Figure US20200087266A1-20200319-C00071
  • wherein m9 is as defined above, m7 is as defined in the aforementioned formula [I-A], and R5, X and Cy are as defined in the aforementioned formula [I], can be obtained. For reduction reaction of the olefin, for example, a hydrogenation reaction only needs to be performed in a single or mixed solvent of tetrahydrofuran, ethyl acetate, ethanol, methanol, water and the like in the presence of a catalyst such as palladium carbon or platinum carbon and the like.
    • EXAMPLES
  • The present invention is explained in more detail in the following by referring to Examples and Experimental Examples, which are not to be construed as limitative.
  • The abbreviations in the Examples are as follows.
    • WSC.HCl: 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride
    • HOBt.H2O: 1-hydroxy-1H-benzotriazole monohydrate
    • DMSO: dimethyl sulfoxide
    • M: mol/L
    Production Example 1 Synthesis of N-(4-chloro-3-{4-[4-(2,2-dimethylpropoxy)phenyl]-6-hydroxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-86)
  • Figure US20200087266A1-20200319-C00072
    • (1) 2-chloro-4-[4-(2,2-dimethylpropoxy)phenyl]-6-methoxy-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00073
  • Under an argon atmosphere, a suspension of 4-(2,2-dimethylpropoxy)phenylboronic acid (2.0 g, 9.6 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (3.5 g, 19 mmol), [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (1.1 g, 0.96 mmol) and 2M aqueous sodium carbonate solution (14 ml, 29 mmol) in toluene (20 ml) was stirred at 100° C. for 3.5 hr. At room temperature, to the reaction mixture were added water and ethyl acetate and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=19/1−4/1) to give the title compound (2.3 g, yield 77%).
  • 1H-NMR (400 MHz, CDCl3) δ: 1.06 (9H, s), 3.68 (2H, s), 4.14 (3H, s), 6.94-7.02 (2H, m), 8.42-8.46 (2H, m).
    • (2) (4-chloro-3-{4-[4-(2,2-dimethylpropoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}phenyl)methanol
  • Figure US20200087266A1-20200319-C00074
  • Under an argon atmosphere, a suspension of 2-chloro-4-[4-(2,2-dimethylpropoxy)phenyl]-6-methoxy-1,3,5-triazine (2.3 g, 7.4 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (1.7 g, 8.9 mmol), [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.61 g, 0.74 mmol) and 2M aqueous sodium carbonate solution (15 ml, mmol) in 1,4-dioxane (23 ml) was stirred at 100° C. for 1.5 hr. At room temperature, to the reaction mixture were added water and ethyl acetate and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=4/1−1/1) to give the title compound (1.3 g, yield 43%).
  • 1H-NMR (400 MHz, CDCl3) δ: 1.06 (9H, s), 1.75 (1H, t, J=5.9 Hz), 3.69 (2H, s), 4.19 (3H, s), 4.77 (2H, d, J=5.9 Hz), 6.98-7.03 (2H, m), 7.46 (1H, dd, J=8.2, 2.2 Hz), 7.53 (1H, d, J=8.2 Hz), 8.00 (1H, d, J=2.2 Hz), 8.52-8.58 (2H, m).
    • (3) tert-butyl N-(4-chloro-3-{4-[4-(2,2-dimethylpropoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-N-(tert-butoxycarbonyl)carbamate
  • Figure US20200087266A1-20200319-C00075
  • Under an argon atmosphere, to a solution of (4-chloro-3-{4-[4-(2,2-dimethylpropoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}phenyl)methanol (1.3 g, 3.2 mmol) obtained in the above-mentioned (2) and triethylamine (0.58 ml, 4.2 mmol) in tetrahydrofuran (13 ml) was added methanesulfonyl chloride (0.29 ml, 3.8 mmol) under ice-cooling, and the mixture was warmed to room temperature. After stirring for 0.5 hr, the reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (13 ml) were added cesium carbonate (3.1 g, 9.5 mmol) and di-tert-butyl iminodicarboxylate (0.83 g, 3.8 mmol), and the mixture was stirred for 3 hr. To the reaction mixture were added water and ethyl acetate and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=9/1−7/3) to give the title compound (1.6 g, yield 82%).
  • 1H-NMR (400 MHz, CDCl3) δ: 1.06 (9H, s), 1.48 (18H, s), 3.69 (2H, s), 4.18 (3H, s), 4.83 (2H, s), 6.96-7.01 (2H, m), 7.39 (1H, dd, J=8.2, 2.2 Hz), 7.48 (1H, d, J=8.2 Hz), 7.98 (1H, d, J=2.2 Hz), 8.51-8.57 (2H, m).
    • (4) 4-chloro-3-{4-[4-(2,2-dimethylpropoxy)phenyl]-6-methoxy-1,3, 5-triazin-2-yl}benzylamine hydrochloride
  • Figure US20200087266A1-20200319-C00076
  • Under an argon atmosphere, to a solution of tert-butyl N-(4-chloro-3-{4-[4-(2,2-dimethylpropoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-N-(tert-butoxycarbonyl)carbamate (1.3 g, 2.2 mmol) obtained in the above-mentioned (3) in 1,4-dioxane (2.8 ml) was added 4M hydrogen chloride/1,4-dioxane solution (11 ml) at room temperature, and the mixture was stirred for 3 hr. The solid was collected by filtration from the suspension, and dried under reduced pressure to give the title compound (0.97 g, yield 99%).
  • 1H-NMR (400 MHz, DMSO-d6) δ: 1.03 (9H, s), 3.76 (2H, s), 4.10-4.18 (2H, m), 4.14 (3H, s), 7.11-7.17 (2H, m), 7.72 (2H, d, J=0.9 Hz), 8.13 (1H, br s), 8.40-8.58 (5H, m).
    • (5) N-(4-chloro-3-{4-[4-(2,2-dimethylpropoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00077
  • Under an argon atmosphere, to a solution of 4-chloro-3-{4-[4-(2,2-dimethylpropoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzylamine hydrochloride (0.97 g, 2.2 mmol) obtained in the above-mentioned (4) and 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.41 g, 2.6 mmol) in N,N-dimethylformamide (10 ml) were added HOBt.H2O (0.43 g, 2.8 mmol), WSC.HCl (2.8 g, 2.8 mmol) and triethylamine (0.91 ml, 6.5 mmol) at room temperature, and the mixture was stirred for 3.5 hr. 3,3,3-Trifluoro-2,2-dimethylpropionic acid (0.067 g, 0.43 mmol), HOBt.H2O (0.066 g, 0.43 mmol) and WSC.HCl (0.082 g, 0.43 mmol) were added, and the mixture was stirred for 1.5 hr. To the reaction mixture were added water and ethyl acetate and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=9/1−7/3) to give the title compound (0.97 g, yield 81%).
  • 1H-NMR (400 MHz, CDCl3) δ: 1.07 (9H, s), 1.44 (6H, s), 3.69 (2H, s), 4.19 (3H, s), 4.55 (2H, d, J=5.8 Hz), 6.22 (1H, br s), 6.96-7.03 (2H, m), 7.34 (1H, dd, J=8.3, 2.3 Hz), 7.51 (1H, d, J=8.3 Hz), 7.91 (1H, d, J=2.3 Hz), 8.50-8.57 (2H, m).
    • (6) N-(4-chloro-3-{4-[4-(2,2-dimethylpropoxy)phenyl]-6-hydroxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-86)
  • Figure US20200087266A1-20200319-C00078
  • Under an argon atmosphere, to a solution of N-(4-chloro-3-{4-[4-(2,2-dimethylpropoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (0.97 g, 1.76 mmol) obtained in the above-mentioned (5) in methanol (10 ml) was added 4M aqueous sodium hydroxide solution (3.5 ml, 14 mmol) at room temperature, and the mixture was stirred at 65° C. for 1.5 hr. To the reaction mixture were added 2M hydrochloric acid (7.0 ml, 14 mmol) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.87 g, yield 92%).
  • 1H-NMR (400 MHz, DMSO-d6) δ: 1.02 (9H, s), 1.37 (6H, s), 3.73 (2H, s), 4.35 (2H, d, J=5.8 Hz), 7.08 (2H, d, J=9.1 Hz), 7.40 (1H, dd, J=8.3, 2.2 Hz), 7.58 (1H, d, J=8.3 Hz), 7.62 (1H, d, J=1.9 Hz), 8.29 (2H, d, J=9.1 Hz), 8.62 (1H, t, J=5.8 Hz), 13.13 (1H, s).
    • Production Example 2 Synthesis of 1-[4-chloro-3-(4-hydroxy-6-phenyl-1,3,5-triazin-2-yl)-benzyl]-3,3-dimethyl-1,3-dihydroindol-2-one (Example No. 1-258)
  • Figure US20200087266A1-20200319-C00079
    • (1) 2-(5-bromomethyl-2-chlorophenyl)-4-methoxy-6-phenyl-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00080
  • By a method similar to that in Production Example 1 (1) and (2), and using 2,4-dichloro-6-methoxy-1,3,5-triazine, 2-chloro-5-hydroxymethylphenylboronic acid, and phenylboronic acid instead of 4-(2,2-dimethylpropoxy)phenylboronic acid, [4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)phenyl]methanol was obtained.
  • Under an argon atmosphere, to a solution of the obtained [4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)phenyl]methanol (0.47 g, 1.4 mmol) and triphenylphosphine (0.56 g, 2.1 mmol) in chloroform (4.5 ml) was added carbon tetrabromide (0.71 g, 2.1 mmol) under ice-cooling. The reaction mixture was stirred at room temperature for 10 min, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=30/1−9/1) to give the title compound (0.49 g, yield 87%).
  • 1H-NMR (400 MHz, CDCl3) δ: 4.22 (3H, s), 4.53 (2H, s), 7.45-7.64 (5H, m), 8.06 (1H, br s), 8.57-8.63 (2H, m).
    • (2) 1-[4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-3,3-dimethyl-1,3-dihydroindol-2-one
  • Figure US20200087266A1-20200319-C00081
  • Under an argon atmosphere, to a solution of 3,3-dimethylindolin-2-one (0.050 g, 0.31 mmol) in N,N-dimethylformamide (1.0 ml) was added sodium hydride (0.012 g, 60 wt % oil dispersion) under ice-cooling. After stirring for min, 2-(5-bromomethyl-2-chlorophenyl)-4-methoxy-6-phenyl-1,3,5-triazine (0.10 g, 0.26 mmol) obtained in the above-mentioned (1) was added, and the mixture was stirred at room temperature for 30 min. To the reaction mixture were added saturated aqueous ammonium chloride solution and ethyl acetate and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=7/2) to give the title compound (0.11 g, yield 89%).
  • 1H-NMR (400 MHz, CDCl3) δ: 1.44 (6H, s), 4.18 (3H, s), 4.98 (2H, s), 6.72-6.76 (1H, m), 7.02-7.08 (1H, m), 7.13-7.19 (1H, m), 7.21-7.25 (1H, m), 7.31-7.36 (1H, m), 7.46-7.53 (3H, m), 7.55-7.61 (1H, m), 8.00 (1H, br s), 8.51-8.58 (2H, m).
    • (3) 1-[4-chloro-3-(4-hydroxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-3,3-dimethyl-1,3-dihydroindol-2-one (Example No. 1-258)
  • Figure US20200087266A1-20200319-C00082
  • Under an argon atmosphere, to a solution of 1-[4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-3,3-dimethyl-1,3-dihydroindol-2-one (0.11 g, 0.23 mmol) obtained in the above-mentioned (2) in methanol (10 ml) was added 4M aqueous sodium hydroxide solution (0.34 ml, 1.4 mmol) at room temperature, and the mixture was stirred at 65° C. for 2 hr. To the reaction mixture were added 10 wt % aqueous citric acid solution (1.4 ml) and water (7.0 ml) at room temperature, and the mixture was stirred for 30 min. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.10 g, yield 96%).
  • 1H-NMR (400 MHz, DMSO-d6) δ: 1.34 (6H, s), 4.99 (2H, s), 6.97 (1H, d, J=7.6 Hz), 7.05 (1H, t, J=7.6 Hz), 7.20 (1H, t, J=7.6 Hz), 7.39 (1H, d, J=7.6 Hz), 7.48 (1H, dd, J=8.3, 1.8 Hz), 7.55 (2H, t, J=7.6 Hz), 7.59-7.68 (2H, m), 7.75 (1H, d, J=1.8 Hz), 8.29 (2H, d, J=7.6 Hz), 13.32 (1H, br s).
    • Production Example 3 Synthesis of N-[4-chloro-3-(4-hydroxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-N-ethyl-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-263)
  • Figure US20200087266A1-20200319-C00083
    • (1) [4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]ethylamine
  • Figure US20200087266A1-20200319-C00084
  • Under an argon atmosphere, to 2-(5-bromomethyl-2-chlorophenyl)-4-methoxy-6-phenyl-1,3,5-triazine (0.20 g, 0.51 mmol) obtained in the same manner as in Production Example 2 (1) was added a solution of 2M ethylamine tetrahydrofuran (2.5 ml) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture were added saturated aqueous sodium hydrogen carbonate and ethyl acetate and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure to give the title compound (0.28 g) as a crude product.
  • 1H-NMR (400 MHz, CDCl3) δ: 1.14 (3H, t, J=7.2 Hz), 2.70 (2H, q, J=7.2 Hz), 3.86 (2H, s), 4.22 (3H, s), 7.44 (1H, dd, J=8.2, 2.2 Hz), 7.48-7.55 (3H, m), 7.57-7.62 (1H, m), 7.97 (1H, d, J=2.2 Hz), 8.58-8.64 (2H, m).
    • (2) N-[4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-N-ethyl-3,3,3-trifluoro-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00085
  • Under an argon atmosphere, to a solution of [4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]ethylamine (0.18 g, 0.38 mmol) obtained in the above-mentioned (1) and 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.12 g, 0.76 mmol) in chloroform (2.0 ml) were added WSC.HCl (0.15 g, 0.76 mmol) and 4-dimethylaminopyridine (0.93 mg, 0.76 mmol) at room temperature, and the mixture was stirred for 16 hr. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=8/3) to give the title compound (0.086 g, yield 46%).
  • 1H-NMR (400 MHz, CDCl3) δ: 1.20 (3H, t, J=6.9 Hz), 1.55 (6H, s), 3.47 (2H, q, J=6.9 Hz), 4.21 (3H, s), 4.71 (2H, s), 7.24-7.30 (1H, m), 7.45-7.63 (4H, m), 7.88 (1H,br s), 8.56-8.64 (2H, m).
    • (3) N-[4-chloro-3-(4-hydroxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-N-ethyl-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-263)
  • Figure US20200087266A1-20200319-C00086
  • Under an argon atmosphere, to a solution of N-[4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-N-ethyl-3,3,3-trifluoro-2,2-dimethylpropionamide (0.086 g, 0.17 mmol) obtained in the above-mentioned (2) in methanol (1.5 ml) was added 4M aqueous sodium hydroxide solution (0.26 ml, 1.0 mmol) at room temperature, and the mixture was stirred at 65° C. for 2 hr. At room temperature, 10 wt % aqueous citric acid solution (1.2 ml) and water (6 ml) were added, and the mixture was stirred for 30 min. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure 30 to give a crude product. To a suspension of the crude product in ethyl acetate (1.5 ml) was added n-hexane (1.5 ml), and the mixture was stirred for 30 min. The solid was collected by filtration, dried under reduced pressure to give the title compound (0.067 g, yield 80%).
  • 1H-NMR (400 MHz, DMSO-d6) δ:1.13 (3H, t, J=6.9 Hz), 1.50 (6H, s), 3.42 (2H, br s), 4.66 (2H, s), 7.41 (1H, dd, J=8.3, 1.8 Hz), 7.56 (2H, t, J=7.9 Hz), 7.61-7.69 (3H, m), 8.34 (2H, d, J=7.9 Hz), 13.33 (1H, br s).
    • Production Example 4 Synthesis of 7-tert-butyl-2-[4-chloro-3-(4-hydroxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-2-azaspiro[3.5]nonan-1-one (Example No. 1-266)
  • Figure US20200087266A1-20200319-C00087
    • (1) methyl 1-benzyloxymethyl-4-tert-butyl-cyclohexanecarboxylate
  • Figure US20200087266A1-20200319-C00088
  • Under an argon atmosphere, to a solution of methyl 4-tert-butyl-cyclohexanecarboxylate (0.46 g, 2.3 mmol) in tetrahydrofuran (2.5 ml) was added dropwise 2M heptane/tetrahydrofuran/ethylbenzene solution (1.4 ml, 2.8 mmol) of lithium diisopropylamide at −78° C. over 5 min. After stirring for 1 hr, benzyl chloromethyl ether (0.38 ml, 2.8 mmol) was added dropwise over 1 min. Under ice-cooling, the mixture was stirred for 1 hr. To the reaction mixture were added 10 wt % aqueous citric acid solution (3.0 ml) and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=30/1) to give the title compound (0.49 g, yield 66%). While the title compound was obtained as a single stereoisomer, the relative configuration thereof is undetermined. Specifically, whether the methoxycarbonyl group is cis/trans relative to the tert-butyl group is undetermined.
  • 1H-NMR (400 MHz, CDCl3) δ: 0.81 (9H, s), 0.88-0.99 (1H, m), 1.00-1.21 (4H, m), 1.68 (2H, d, J=12.0 Hz), 2.29 (2H, d, J=12.0 Hz), 3.36 (2H, s), 3.69 (3H, s), 4.48 (2H, br s), 7.22-7.38 (5H, m).
    • (2) methyl 4-tert-butyl-1-hydroxymethyl-cyclohexanecarboxylate
  • Figure US20200087266A1-20200319-C00089
  • Under an argon atmosphere, to a solution of methyl 1-benzyloxymethyl-4-tert-butyl-cyclohexanecarboxylate (0.49 g, 1.5 mmol) obtained in the above-mentioned (1) in methanol (5.5 ml) was added ASCA-2 (4.5% palladium of activated carbon support-0.5% platinum catalyst (see N.E. CHEMCAT, Fine chemical October 1, 2002, pages 5-14), 0.20 g) at room temperature. Under 1 atm hydrogen, the mixture was stirred for 4 hr. Under an argon atmosphere, the reaction mixture was filtered through celite, and the filtrate was eluted with ethyl acetate. The filtrate was concentrated under reduced pressure to give the title compound (0.27 g, yield 75%). While the title compound is a single stereoisomer, the relative configuration thereof is undetermined.
  • 1H-NMR (400 MHz, CDCl3) δ: 0.83 (9H, s), 0.91-1.17 (5H, m), 1.64-1.78 (3H, m), 2.20-2.31 (2H, m), 3.53 (2H, d, J=6.0 Hz), 3.73 (3H, s).
    • (3) 4-tert-butyl-1-hydroxymethyl-cyclohexanecarboxylic acid
  • Figure US20200087266A1-20200319-C00090
  • Under an argon atmosphere, to a solution of methyl 4-tert-butyl-1-hydroxymethyl-cyclohexanecarboxylate (0.27 g, 1.2 mmol) obtained in the above-mentioned (2) in methanol (1.7 ml) were added tetrahydrofuran (1.7 ml) and 4M aqueous sodium hydroxide solution (1.7 ml, 7.0 mmol) at room temperature, and the mixture was stirred at 65° C. for 1.5 hr. Methanol (1.7 ml), tetrahydrofuran (1.7 ml) and 4M aqueous sodium hydroxide solution (1.7 ml, 7.0 mmol) were added, and the mixture was stirred at 65° C. for 2 hr. To the reaction mixture were added 2M hydrochloric acid (7.5 ml, 15 mmol) and water at room temperature, and the mixture was stirred. Ethyl acetate was added and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=12/1) to give the title compound (0.24 g, yield 94%). While the title compound is a single stereoisomer, the relative configuration thereof is undetermined.
  • 1H-NMR (400 MHz, DMSO-d6) δ: 0.80 (9H, s), 0.86-1.12 (5H, m), 1.53-1.66 (2H, m), 2.00-2.13 (2H, m), 3.31 (2H, s).
    • (4) 4-tert-butyl-1-hydroxymethyl-cyclohexanecarboxylic acid 4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzylamide
  • Figure US20200087266A1-20200319-C00091
  • By a method similar to that in Production Example 1 (1)-(4), and using 2,4-dichloro-6-methoxy-1,3,5-triazine, 2-chloro-5-hydroxymethylphenylboronic acid, and phenylboronic acid instead of 4-(2,2-dimethylpropoxy)phenylboronic acid, 4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzylamine hydrochloride was obtained.
  • Under an argon atmosphere, to a solution of the obtained 4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzylamine hydrochloride (0.90 g, 0.25 mmol) and 4-tert-butyl-1-hydroxymethyl-cyclohexanecarboxylic acid (0.080 g, 0.37 mmol) obtained in the above-mentioned (3) in N,N-dimethylformamide (2.0 ml) were added HOBt.H2O (0.057 g, 0.37 mmol), WSC.HCl (0.071 g, 0.37 mmol) and triethylamine (0.10 ml, 0.74 mmol) at room temperature, and the mixture was stirred for 13 hr. To the reaction mixture were added saturated aqueous sodium hydrogen carbonate and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=1/2−1/3) to give the title compound (0.11 g, yield 81%). While the title compound is a single stereoisomer, the relative configuration thereof is undetermined.
  • 1H-NMR (400 MHz, CDCl3) δ: 0.78 (9H, s), 0.94-1.22 (5H, m), 1.66-1.75 (2H, m), 2.22-2.30 (2H, m), 2.42 (1H, t, J=5.0 Hz), 3.52 (2H, d, J=5.0 Hz), 4.21 (3H, s), 4.57 (2H, d, J=5.8 Hz), 6.46 (1H, t, J=5.8 Hz), 7.38 (1H, dd, J=8.3, 2.3 Hz), 7.47-7.55 (3H, m), 7.57-7.62 (1H, m), 7.97 (1H, d, J=2.3 Hz), 8.57-8.62 (2H, m).
    • (5) 7-tert-butyl-2-[4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-2-azaspiro[3.5]nonan-1-one
  • Figure US20200087266A1-20200319-C00092
  • Under an argon atmosphere, to a solution of 4-tert-butyl-1-hydroxymethyl-cyclohexanecarboxylic acid 4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzylamide (0.11 g, 0.20 mmol) obtained in the above-mentioned (4) and triphenylphosphine (0.080 g, 0.30 mmol) in tetrahydrofuran (1.0 ml) was added bis(2-methoxyethyl) azodicarboxylate (0.071 g, 0.30 mmol) at room temperature, and the mixture was stirred for 1.5 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by preparative thin layer chromatography (eluent: n-hexane/ethyl acetate=4/1) to give the title compound (0.068 g, yield 66%). While the title compound is a single stereoisomer, the relative configuration of the tert-butyl group is undetermined.
  • 1H-NMR (400 MHz, CDCl3) δ: 0.81-1.77 (7H, m), 0.87 (9H, s), 2.03-2.12 (2H, m), 2.87 (2H, br s), 4.21 (3H, s), 4.40 (2H, br s), 7.30-7.37 (1H, m), 7.48-7.64 (4H, m), 7.90 (1H, br s), 8.57-8.63 (2H, m).
    • (6) 7-tert-butyl-2-[4-chloro-3-(4-hydroxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-2-azaspiro[3.5]nonan-1-one (Example No. 1-266)
  • Figure US20200087266A1-20200319-C00093
  • Under an argon atmosphere, to a solution of 7-tert-butyl-2-[4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-2-azaspiro[3.5]nonan-1-one (0.068 g, 0.13 mmol) obtained in the above-mentioned (5) in methanol (1.2 ml) was added 4M aqueous sodium hydroxide solution (0.20 ml, 0.81 mmol) at room temperature, and the mixture was stirred at 65° C. for 1.5 hr. At room temperature, to the reaction mixture were added 10 wt % aqueous citric acid solution (0.82 ml) and water (4.0 ml), and the mixture was stirred for 30 min. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.062 g, yield 94%). While the title compound is a single stereoisomer, the relative configuration of the tert-butyl group is undetermined.
  • 1H-NMR (400 MHz, DMSO-d6) δ: 0.83 (9H, s), 0.90-0.99 (1H, m), 1.41-1.67 (6H, m), 1.96-2.03 (2H, m), 2.92 (2H, s), 4.38 (2H, s), 7.47 (1H, dd, J=8.3, 1.8 Hz), 7.56 (2H, t, J=7.6 Hz), 7.63-7.69 (3H, m), 8.34 (2H, d, J=7.6 Hz), 13.34 (1H, br s).
    • Production Example 5 Synthesis of 4-[2-(6-methylpyridin-2-ylmethoxy)-6-trifluoromethylphenyl]-6-(4-phenylethynylphenyl)-1,3,5-triazin-2-ol hydrochloride (Example No. 2-98)
  • Figure US20200087266A1-20200319-C00094
    • (1) 2-bromo-1-methoxymethoxy-3-trifluoromethyl-benzene
  • Figure US20200087266A1-20200319-C00095
  • Under an argon atmosphere, to a solution of 2-bromo-3-fluorobenzotrifluoride (6.0 g, 25 mmol) and 2-(methylsulfonyl)ethanol (4.3 g, 35 mmol) in N,N-dimethylformamide (10 ml) was added sodium hydride (2.8 g, 60 wt % oil dispersion) in 3 portions under ice-cooling. After stirring at room temperature for 10 min, chloromethyl methyl ether (5.3 ml, 69 mmol) was added dropwise under ice-cooling. After stirring for 30 min, the mixture was stirred at room temperature for 15 min. Under ice-cooling, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=12/1) to give the title compound (5.0 g, yield 70%).
  • 1H-NMR (400 MHz, CDCl3) δ: 3.53 (3H, s), 5.29 (2H, s), 7.31-7.38 (3H, m).
    • (2) 2-(2-methoxymethoxy-6-trifluoromethylphenyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane
  • Figure US20200087266A1-20200319-C00096
  • Under an argon atmosphere, to a solution of 2-bromo-1-methoxymethoxy-3-trifluoromethyl-benzene (4.9 g, 17 mmol) obtained in the above-mentioned (1) in tetrahydrofuran (90 ml) was added dropwise n-butyllithium (1.6M n-hexane solution, 11 ml, 17 mmol) at −78° C. over 30 min. 2-Isopropoxy-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (3.5 ml, 17 mmol) was added dropwise over 15 min, and the mixture was warmed to room temperature and stirred for 2 hr. To the reaction mixture were added saturated ammonium chloride aqueous solution and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=9/1) to give the title compound (2.8 g, yield 48%).
  • 1H-NMR (400 MHz, CDCl3) δ: 1.39 (12H, s), 3.47 (3H, s), 5.18 (2H, s), 7.20 (1H, d, J=8.4 Hz), 7.24-7.28 (1H, m), 7.36-7.42 (1H, m).
    • (3) 2-(4-benzyloxyphenyl)-4-methoxy-6-(2-methoxymethoxy-6-trifluoromethylphenyl)-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00097
  • By a method similar to that in Production Example 1 (1), and using 2,4-dichloro-6-methoxy-1,3,5-triazine, and 4-(benzyloxy)phenylboronic acid instead of 4-(2,2-dimethylpropoxy)phenylboronic acid, 2-(4-benzyloxyphenyl)-4-chloro-6-methoxy-1,3,5-triazine was obtained.
  • Under an argon atmosphere, to a solution of the obtained 2-(4-benzyloxyphenyl)-4-chloro-6-methoxy-1,3,5-triazine (3.0 g, 9.2 mmol) and 2-(2-methoxymethoxy-6-trifluoromethylphenyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (2.8 g, 8.4 mmol) obtained in the above-mentioned (2) in N,N-dimethylformamide (25 ml) were added [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (1.4 g, 1.7 mmol), copper(I) iodide (0.48 g, 2.5 mmol) and 2M aqueous sodium carbonate solution (13 ml, 25 mmol), and the mixture was stirred at 115° C. for 45 min. To the reaction mixture were added water and ethyl acetate. After stirring, the insoluble material was removed by celite filtration, and the filtrate was eluted with ethyl acetate. The filtrate was partitioned, and the organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=7/2) to give the title compound (2.0 g, yield 47%).
  • 1H-NMR (400 MHz, DMSO-d6) δ: 3.39 (3H, s), 4.14 (3H, s), 5.13 (2H, s), 5.15 (2H, s), 7.02-7.08 (2H, m), 7.30-7.46 (7H, m), 7.48-7.55 (1H, m), 8.47-8.52 (2H, m).
    • (4) 4-[4-methoxy-6-(2-methoxymethoxy-6-trifluoromethylphenyl)-1,3,5-triazin-2-yl]phenol
  • Figure US20200087266A1-20200319-C00098
  • Under an argon atmosphere, to a solution of 2-(4-benzyloxyphenyl)-4-methoxy-6-(2-methoxymethoxy-6-trifluoromethylphenyl)-1,3,5-triazine (2.0 g, 4.0 mmol) obtained in the above-mentioned (3) in ethyl acetate (10 ml) were added methanol (10 ml) and 10 wt % palladium carbon (0.49 g) at room temperature. Under 1 atm hydrogen, the mixture was stirred for 2 hr. Under an argon atmosphere, the reaction mixture was filtered through celite, and the filtrate was eluted with ethyl acetate. The filtrate was concentrated under reduced pressure to give the title compound (1.6 g, yield 97%).
  • 1H-NMR(400 MHz, CDCl3)δ:3.39 (3H, s), 4.14 (4H, s), 5.13 (2H, s), 5.39 (1H, br s), 6.87-6.93 (2H, m), 7.40-7.45 (2H, m), 7.48-7.55 (1H, m), 8.43-8.48 (2H, m).
    • (5) trifluoromethanesulfonic acid 4-[4-methoxy-6-(2-methoxymethoxy-6-trifluoromethylphenyl)-1,3,5-triazin-2-yl]phenyl ester
  • Figure US20200087266A1-20200319-C00099
  • Under an argon atmosphere, to a solution of 4-[4-methoxy-6-(2-methoxymethoxy-6-trifluoromethylphenyl)-1,3,5-triazin-2-yl]phenol (1.6 g, 3.9 mmol) obtained in the above-mentioned (4) in pyridine (15 ml) was added dropwise trifluoromethanesulfonic anhydride (13 ml, 7.7 mmol) under ice-cooling, and the mixture was stirred at room temperature for 30 min. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=3/1) to give the title compound (2.0 g, yield 95%).
  • 1H-NMR (400 MHz, CDCl3) δ: 3.39 (3H, s), 4.17 (3H, s), 5.13 (2H, s), 7.37-7.48 (4H, m), 7.51-7.58 (1H, m), 8.61-8.67 (2H, m).
    • (6) 2-methoxy-4-(2-methoxymethoxy-6-trifluoromethylphenyl)-6-(4-phenylethynylphenyl)-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00100
  • Under an argon atmosphere, to a solution of trifluoromethanesulfonic acid 4-[4-methoxy-6-(2-methoxymethoxy-6-trifluoromethylphenyl)-1,3,5-triazin-2-yl]phenyl ester (0.50 g, 0.93 mmol) obtained in the above-mentioned (5), bis(triphenylphosphine)palladium(II)dichloride (0.098 g, 0.139 mmol) and copper(I) iodide (0.053 g, 0.28 mmol) in N,N-dimethylformamide (5.0 ml) were added triethylamine (0.39 ml, 2.8 mmol) and ethynylbenzene (0.51 ml, 4.6 mmol), and the mixture was stirred at 65° C. for 2.5 hr. To the reaction mixture were added water and ethyl acetate. After stirring for 1 hr, the insoluble material was removed by celite filtration, and the filtrate was eluted with ethyl acetate. The filtrate was partitioned, and the organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=5/1−4/1) to give the title compound (0.45 g, yield 98%).
  • 1H-NMR (400 MHz, DMSO-d6) δ: 3.40 (3H, s), 4.17 (3H, s), 5.14 (2H, s), 7.34-7.39 (3H, m), 7.42-7.47 (2H, m), 7.50-7.59 (3H, m), 7.62-7.67 (2H, m), 8.50-8.55 (2H, m).
    • (7) 2-[4-methoxy-6-(4-phenylethynylphenyl)-1,3,5-triazin-2-yl]-3-trifluoromethylphenol
  • Figure US20200087266A1-20200319-C00101
  • Under an argon atmosphere, to a solution of 2-methoxy-4-(2-methoxymethoxy-6-trifluoromethylphenyl)-6-(4-phenylethynylphenyl)-1,3,5-triazine (0.45 g, 0.92 mmol) obtained in the above-mentioned (6) in methanol (4.5 ml) were added 1,4-dioxane (4.5 ml) and methanesulfonic acid (0.030 ml, 0.46 mmol) at room temperature. The mixture was stirred at 70° C. for 5 hr, and triethylamine (0.13 ml, 0.92 mmol) was added to the reaction mixture at room temperature. To the reaction mixture was added water (45 ml), and the mixture was stirred for 30 min. The precipitated solid was collected by filtration and dried to give the title compound (0.38 g, yield 93%).
  • 1H-NMR (400 MHz, CDCl3) δ: 4.23 (3H, s), 7.25-7.30 (1H, m), 7.36-7.40 (3H, m), 7.43-7.47 (1H, m), 7.50-7.60 (3H, m), 7.67-7.72 (2H, m), 8.48-8.52 (2H, m), 12.43 (1H, br s)
    • (8) 2-methoxy-4-[2-(6-methylpyridin-2-ylmethoxy)-6-trifluoromethylphenyl]-6-(4-phenylethynylphenyl)-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00102
  • Under an argon atmosphere, to a solution of 2-[4-methoxy-6-(4-phenylethynylphenyl)-1,3,5-triazin-2-yl]-3-trifluoromethylphenol (0.24 g, 0.54 mmol) obtained in the above-mentioned (7), 6-methyl-2-pyridinemethanol (0.099 g, 0.80 mmol) and triphenylphosphine (0.21 g, 0.80 mmol) in tetrahydrofuran (6.0 ml) was added bis(2-methoxyethyl) azodicarboxylate (0.19 g, 0.80 mmol) in 3 portions under ice-cooling. The reaction mixture was stirred for 20 min and at room temperature for 20 hr. Thereafter, to the reaction mixture were added 6-methyl-2-pyridinemethanol (0.099 g, 0.80 mmol) and triphenylphosphine (0.21 g, 0.80 mmol), and bis(2-methoxyethyl) azodicarboxylate (0.19 g, 0.80 mmol) in 2 portions under ice-cooling. After stirring for 20 min, the reaction mixture was stirred for 10 min at room temperature. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=4/3) to give the title compound (0.28 g, yield 95%).
  • 1H-NMR (400 MHz, CDCl3) δ: 2.51 (3H, s), 4.17 (3H, s), 5.21 (2H, s), 6.96-7.01 (1H, m), 7.02-7.07 (1H, m), 7.20-7.25 (1H, m), 7.33-7.42 (5H, m), 7.47-7.59 (3H, m), 7.62-7.68 (2H, m), 8.52-8.57 (2H, m).
    • (9) 4-[2-(6-methylpyridin-2-ylmethoxy)-6-trifluoromethylphenyl]-6-(4-phenylethynylphenyl)-1,3,5-triazin-2-ol
  • Figure US20200087266A1-20200319-C00103
  • Under an argon atmosphere, to a suspension of 2-methoxy-4-[2-(6-methylpyridin-2-ylmethoxy)-6-trifluoromethylphenyl]-6-(4-phenylethynylphenyl)-1,3,5-triazine (0.28 g, 0.52 mmol) obtained in the above-mentioned (8) in methanol (4.6 ml) were added 4M aqueous sodium hydroxide solution (0.77 ml, 3.1 mmol) and tetrahydrofuran (0.46 ml) at room temperature. At 65° C., the reaction mixture was stirred for 3.5 hr. To the reaction mixture were added 10 wt % aqueous citric acid solution (3.2 ml) and water (16 ml) at room temperature, and the mixture was stirred for 30 min. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.27 g, yield 95%).
  • 1H-NMR (400 MHz, DMSO-d6) δ: 2.43 (3H, s), 5.31 (2H, s), 7.07-7.17 (2H, m), 7.43-7.49 (3H, m), 7.50-7.68 (5H, m), 7.69-7.82 (3H, m), 8.32-8.38 (2H, m), 13.63 (1H, br s).
    • (10) 4-[2-(6-methylpyridin-2-ylmethoxy)-6-trifluoromethylphenyl]-6-(4-phenylethynylphenyl)-1,3,5-triazin-2-01 hydrochloride (Example No. 2-98)
  • Figure US20200087266A1-20200319-C00104
  • Under an argon atmosphere, to a solution of 4-[2-(6-methylpyridin-2-ylmethoxy)-6-trifluoromethylphenyl]-6-(4-phenylethynylphenyl)-1,3,5-triazin-2-ol (0.27 g, 0.49 mmol) obtained in the above-mentioned (9) in 1,4-dioxane (5.3 ml) was added 4M hydrogen chloride/1,4-dioxane solution (0.37 ml, 1.5 mmol) at room temperature. To the reaction mixture was added n-hexane (21 ml), and the mixture was stirred for 30 min. The precipitated solid was collected by filtration, washed with n-hexane, and dried under reduced pressure to give the title compound (0.26 g, yield 91%).
  • 1H-NMR (400 MHz, DMSO-d6) δ: 2.48 (3H, s), 5.37 (2H, s), 7.23 (1H, d, J=7.3 Hz), 7.28 (1H, d, J=7.3 Hz), 7.48-7.45 (3H, m), 7.56 (1H, d, J=7.9 Hz), 7.64-7.59 (2H, m), 7.67 (1H, d, J=8.6 Hz), 7.82-7.72 (4H, m), 8.35 (2H, dd, J=6.8, 2.0 Hz).
    • Production Example 6 Synthesis of 2-[4-chloro-2-methyl-5-(4,4,5,5-tetramethyl[1,3,2]dioxaborolan-2-yl)benzyloxy]tetrahydropyran (1) 4-chloro-5-iodo-2-methylbenzoic acid
  • Figure US20200087266A1-20200319-C00105
  • Under an argon atmosphere, to 4-chloro-2-methylbenzoic acid (1.9 g, 11 mmol) were added concentrated sulfuric acid (16 ml) and N-iodosuccinimide (2.7 g, 12 mmol) under ice-cooling, and the mixture was stirred at room temperature for 4 hr. The reaction mixture was carefully poured into ice water and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (3.3 g, yield 99%).
  • 1H-NMR (CDCl3) δ: 2.58 (3H, s), 7.38 (1H, br s), 8.50 (1H, s).
    • (2) (4-chloro-5-iodo-2-methylphenyl)methanol
  • Figure US20200087266A1-20200319-C00106
  • Under an argon atmosphere, to a solution of 4-chloro-5-iodo-2-methylbenzoic acid (2.4 g, 8.1 mmol) in tetrahydrofuran (12 ml) were added triethylamine (1.2 ml, 8.9 mmol) and isobutyl chloroformate (1.2 ml, 8.9 mmol) under ice-cooling, and the mixture was stirred for 30 min. At room temperature, the insoluble material was removed by filtration, and washed with tetrahydrofuran (36 ml). The filtrate was added dropwise to a solution of prepared sodium borohydride (0.92 g, 24 mmol) in water (4.5 ml) over 10 min under ice-cooling. After stirring at room temperature for 2 hr, to the reaction mixture was added sodium borohydride (0.30 g, 8.1 mmol), and the mixture was stirred for 1 hr. To the reaction mixture were added saturated aqueous ammonium chloride solution and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: chloroform/ethyl acetate=100/0−95/5) to give the title compound (2.0 g, yield 88%).
  • 1H-NMR (400 MHz, CDCl3) δ: 1.60 (1H, t, J=5.7 Hz), 2.26 (3H, s), 4.63 (2H, d, J=5.6 Hz), 7.25-7.26 (1H, m), 7.84 (1H, br s).
    • (3) 2-(4-chloro-5-iodo-2-methylbenzyloxy)tetrahydropyran
  • Figure US20200087266A1-20200319-C00107
  • Under an argon atmosphere, to a solution of (4-chloro-5-iodo-2-methylphenyl)methanol (2.0 g, 7.1 mmol) obtained in the above-mentioned (1) in chloroform (20 ml) were added pyridinium p-toluenesulfonate (0.27 mg, 1.1 mmol) and 3,4-dihydro-2H-pyran (0.97 ml, 11 mmol) at room temperature, and the mixture was stirred for 16 hr. The reaction mixture was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=9/1) to give the title compound (2.6 g, yield 99%).
  • 1H-NMR (400 MHz, CDCl3) δ: 1.51-1.92 (6H, m), 2.26 (3H, s), 3.52-3.59 (1H, m), 3.85-3.91 (1H, m), 4.38 (1H, d, J=12.6 Hz), 4.67-4.72 (2H, m), 7.25 (1H, br s), 7.82 (1H, br s).
    • (4) 2-[4-chloro-2-methyl-5-(4,4,5,5-tetramethyl[1,3,2]dioxaborolan-2-yl)benzyloxy]tetrahydropyran
  • Figure US20200087266A1-20200319-C00108
  • Under an argon atmosphere, to a solution of 2-(4-chloro-5-iodo-2-methylbenzyloxy)tetrahydropyran (2.3 g, 6.2 mmol) obtained in the above-mentioned (2) in 1,4-dioxane (23 ml) were added biphenyl-2-yl-dicyclohexylphosphine (0.43 g, 1.2 mmol), palladium(II) acetate (0.070 g, 0.31 mmol), triethylamine (3.4 ml, 25 mmol) and 4,4,5,5-tetramethyl-1,3,2-dioxaborolane (2.7 ml, 18 mmol) at room temperature, and the mixture was stirred at 80° C. for 5 hr. Under ice-cooling, to the reaction mixture was added dropwise water, and ethyl acetate was added. After partitioning, the organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=98/2−80/20) to give the title compound (1.3 g, yield 60%).
  • 1H-NMR (400 MHz, CDCl3) δ: 1.36 (12H, s), 1.47-1.90 (6H, m), 2.34 (3H, s), 3.52-3.59 (1H, m), 3.88-3.95 (1H, m), 4.42 (1H, d, J=11.6 Hz), 4.67 (1H, t, J=3.5 Hz), 4.74 (1H, d, J=11.6 Hz), 7.18 (1H, br s), 7.63 (1H, br s).
    • Production Example 7 Synthesis of tert-butyl-(4-chloro-3-iodo-2-methylbenzyloxy)dimethylsilane (1) 3-(tert-butyl-dimethylsilanyloxymethyl)-6-chloro-2-methylphenylamine
  • Figure US20200087266A1-20200319-C00109
  • Under an argon atmosphere, to a solution of 3-(tert-butyldimethylsilanyloxymethyl)-2-methyl-phenylamine (0.91 g, 3.6 mmol) in tetrahydrofuran (5.0 ml) was added N-chlorosuccinimide (0.48 g, 3.6 mmol) at room temperature. After stirring for 22 hr, to the reaction mixture was added n-hexane (10 ml), and the insoluble material was filtered off. The filtrate was concentrated, and purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=20/1) to give the title compound (0.18 g, yield 17%).
  • 1H-NMR(400 MHz, CDCl3)δ:0.08 (6H, s), 0.92 (9H, s), 2.11 (3H, s), 4.01 (2H, br s), 4.60-4.69 (2H, m), 6.77 (1H, d, J=8.4 Hz), 7.11 (1H, d, J=8.4 Hz).
    • (2) tert-butyl-(4-chloro-3-iodo-2-methylbenzyloxy)dimethylsilane
  • Figure US20200087266A1-20200319-C00110
  • Under an argon atmosphere, to a solution of 3-(tert-butyl-dimethylsilanyloxymethyl)-6-chloro-2-methylphenylamine (0.18 g, 0.63 mmol) obtained in the above-mentioned (1) in acetonitrile (2.0 ml) were added iodine (0.19 g, 0.76 mmol) and tert-butyl nitrite (0.11 ml, 0.94 mmol) at room temperature, and the mixture was stirred at 65° C. for 30 min. At room temperature, to the reaction mixture were added water and ethyl acetate. After partitioning, the organic layer was washed with saturated aqueous sodium hydrogen carbonate, 10 wt % aqueous sodium thiosulfate solution, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=40/1) to give the title compound (0.099 g, yield 40%).
  • 1H-NMR(400 MHz, CDCl3)δ:0.10 (6H, s), 0.93 (9H, s), 2.47 (3H, s), 4.68 (2H, s), 7.30 (1H, d, J=8.4 Hz), 7.35 (1H, d, J=8.1 Hz).
    • Production Example 8 Synthesis of 2-(6-chloro-2-methoxymethoxy-3-methylphenyl)-4,4,5,5-tetramethyl[1,3,2]dioxaborolane (1) 4-chloro-2-methoxymethoxy-1-methylbenzene
  • Figure US20200087266A1-20200319-C00111
  • Under an argon atmosphere, to a solution of 5-chloro-2-methylphenol (1.0 g, 7.0 mmol) in N,N-dimethylformamide (20 ml) was added sodium hydride (0.34 g, 60 wt % oil dispersion) under ice-cooling. After stirring for 15 min, the mixture was stirred at room temperature for 30 min. Under ice-cooling, chloromethyl methyl ether (0.64 ml, 8.4 mmol) was added, and the mixture was stirred for 30 min. To the reaction mixture were added water and diethyl ether, and the mixture was partitioned at room temperature. The organic layer was washed with water, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/diethyl ether=25/1) to give the title compound (1.3 g, yield 96%).
  • 1H-NMR(400 MHz, CDCl3)δ:2.20 (3H, s), 3.48 (3H, s), 5.18 (2H, s), 6.89 (1H, dd, J=7.9, 2.0 Hz), 7.03-7.07 (2H, m).
    • (2) 2-(6-chloro-2-methoxymethoxy-3-methylphenyl)-4,4,5,5-tetramethyl[1,3,2]dioxaborolane
  • Figure US20200087266A1-20200319-C00112
  • Under an argon atmosphere, to a solution of 4-chloro-2-methoxymethoxy-1-methylbenzene (0.75 g, 4.0 mmol) obtained in the above-mentioned (1) in tetrahydrofuran (20 ml) was added dropwise n-butyllithium (1.6M n-hexane solution, 2.5 ml, 4.0 mmol) at −78° C. over 5 min. After stirring for 30 min, 2-isopropoxy-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (0.81 ml, 4.0 mmol) was added. After stirring for 2 hr, the stirring was discontinued, and the mixture was warmed to room temperature. After 13 hr, to the reaction mixture were added saturated aqueous ammonium chloride solution and ethyl acetate, and the mixture was partitioned. Thereafter, the organic layer was washed with water, washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=12/1) to give the title compound (0.20 g, yield 15%).
  • 1H-NMR (400 MHz, CDCl3) δ: 1.40 (12H, s), 2.27 (3H, s), 3.55 (3H, s), 5.03 (2H, s), 7.01 (1H, d, J=8.2 Hz), 7.07-7.11 (1H, m).
    • Production Example 9 Synthesis of N-{4-chloro-3-[4-(4-isobutylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-2,2-dimethylpropionamide (Example No. 1-51)
  • Figure US20200087266A1-20200319-C00113
    • (1) 2-chloro-4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00114
  • Under an argon atmosphere, a suspension of 4-(2-methylpropyl)phenylboronic acid (35 g, 200 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (46 g, 260 mmol), tetrakis(triphenylphosphine)palladium(0) (2.3 g, 2.0 mmol) and sodium carbonate (63 g, 590 mmol) in toluene (280 ml) and distilled water (280 ml) was stirred at 70° C. for 3.5 hr. At room temperature, to the reaction mixture were added water, ethyl acetate, and n-hexane, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure to give the title compound (60 g) as a crude product.
    • (2) {4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol
  • Figure US20200087266A1-20200319-C00115
  • Under an argon atmosphere, a suspension of a crude product (60 g) of 2-chloro-4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazine obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (44 g, 240 mmol), [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (3.2 g, 3.9 mmol) and cesium fluoride (90 g, 590 mmol) in acetonitrile (440 ml) and distilled water (130 ml) was stirred at 67° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water, and the organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=7/3−6/4) to give the title compound (57 g).
  • 1H-NMR (CDCl3) δ: 0.93 (6H, d, J=6.6 Hz), 1.77 (1H, t, J=6.1 Hz), 1.90-1.97 (1H, m), 2.57 (2H, d, J=7.3 Hz), 4.21 (3H, s), 4.77 (2H, d, J=6.1 Hz), 7.29 (2H, d, J=8.3 Hz), 7.47 (1H, dd, J=8.3, 2.1 Hz), 7.54 (1H, d, J=8.3 Hz), 8.01 (1H, d, J=2.1 Hz), 8.51 (2H, d, J=8.3 Hz).
    • (3) tert-butyl N-{4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate
  • Figure US20200087266A1-20200319-C00116
  • Under an argon atmosphere, to a solution of {4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.25 g, 0.64 mmol) obtained in the above-mentioned (2) and triphenylphosphine (0.25 g, 0.96 mmol) in chloroform (2.4 ml) was added carbon tetrabromide (0.32 g, 0.96 mmol) under ice-cooling. The reaction mixture was stirred at room temperature for 10 min. The reaction mixture was subject to silica gel column chromatography (eluent: n-hexane/ethyl acetate=30/1−10/1), and concentrated under reduced pressure. A solution of the residue in N,N-dimethylformamide (2.0 ml) was added to a solution of di-tert-butyl iminodicarboxylate (0.140 g, 0.64 mmol) and sodium hydride (0.026 g, 60 wt % oil dispersion) in N,N-dimethylformamide (1.0 ml) under ice-cooling, and the mixture was stirred at room temperature for 15 min. The reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water, and the organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=6/1) to give the title compound (0.27 g, yield 72%).
  • 1H-NMR (CDCl3) δ: 0.93 (6H, d, J=6.6 Hz), 1.47 (18H, s), 1.88-1.98 (1H, m), 2.57 (2H, d, J=7.3 Hz), 4.19 (3H, s), 4.83 (2H, s), 7.28 (2H, d, J=8.4 Hz), 7.39 (1H, dd, J=8.4, 2.3 Hz), 7.48 (1H, d, J=8.4 Hz), 8.00 (1H, d, J=2.3 Hz), 8.50 (2H, dt, J=8.4, 1.8 Hz).
    • (4) 4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride
  • Figure US20200087266A1-20200319-C00117
  • Under an argon atmosphere, to tert-butyl N-{4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate (0.27 g, 0.46 mmol) obtained in the above-mentioned (3) was added 4M hydrogen chloride/1,4-dioxane solution (2.0 ml) at room temperature, and the mixture was stirred for 30 min. The solid was collected by filtration from the suspension, and dried under reduced pressure to give the title compound as a crude product (0.16 g).
    • (5) N-{4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00118
  • Under an argon atmosphere, to a solution of a crude product (0.035 g) of 4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride obtained in the above-mentioned (4), HOBt.H2O (0.019 g, 0.12 mmol) and WSC.HCl (0.024 g, 0.13 mmol) in N,N-dimethylformamide (1.0 ml) were added 2,2-dimethylpropionic acid (0.014 ml, 0.12 mmol) and triethylamine (0.035 ml, 0.25 mmol) at room temperature, and the mixture was stirred for 3 hr. To the reaction mixture were added saturated aqueous sodium hydrogen carbonate and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=3/2) to give the title compound (0.030 g).
  • 1H-NMR (CDCl3) δ: 0.93 (6H, d, J=6.6 Hz), 1.24 (9H, s), 1.88-1.99 (1H, m), 2.57 (2H, d, J=7.1 Hz), 4.20 (3H, s), 4.50 (2H, d, J=6.0 Hz), 5.98 (1H, br s), 7.29 (2H, d, J=8.3 Hz), 7.36 (1H, dd, J=8.2, 2.3 Hz), 7.51 (1H, d, J=8.2 Hz), 7.92 (1H, d, J=2.3 Hz), 8.50 (2H, d, J=8.3 Hz).
    • (6) N-{4-chloro-3-[4-hydroxy-6-(4-isobutylphenyl)-1,3,5-triazin-2-yl]benzyl}-2,2-dimethylpropionamide (Example No. 1-51)
  • Figure US20200087266A1-20200319-C00119
  • Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-2,2-dimethylpropionamide (0.030 g, 0.064 mmol) obtained in the above-mentioned (5) in methanol (10 ml) was added 4M aqueous sodium hydroxide solution (0.096 ml) at room temperature, and the mixture was stirred at room temperature for 16 hr. To the reaction mixture were added 10% aqueous citric acid solution (0.38 ml) and water (2.3 ml) at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.026 g, yield 90%). A suspension of the title compound (0.030 g) in DME (0.60 ml) was stirred at room temperature, and the solid was collected by filtration and dried to give the title compound as crystals (0.026 g).
    • Production Example 10 Synthesis of N-{4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-81)
  • Figure US20200087266A1-20200319-C00120
    • (1) 2-chloro-4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00121
  • Under an argon atmosphere, to a suspension of 3-fluoro-4-methylphenylboronic acid (0.43 g, 2.8 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (1.0 g, 5.6 mmol) and tetrakis(triphenylphosphine)palladium(0) (0.16 g, 0.14 mmol) in toluene (8 ml) was added 2M aqueous tripotassium phosphate solution (4.0 ml) at room temperature, and the mixture was stirred at 100° C. for 3 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water, partitioned, washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/chloroform=2/3−1/2) to give the title compound (0.58 g, yield 81%).
  • 1H-NMR (CDCl3) δ: 2.37 (3H, d, J=2.1 Hz), 4.17 (3H, s), 7.32 (1H, t, J=7.9 Hz), 8.12 (1H, dd, J=10.7, 1.7 Hz), 8.19 (1H, dd, J=7.9, 1.7 Hz).
    • (2) {4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol
  • Figure US20200087266A1-20200319-C00122
  • Under an argon atmosphere, to a solution of 2-chloro-4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazine (0.58 g, 2.3 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.51 g, 2.7 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.18 g, 0.23 mmol) in 1,4-dioxane (9.0 ml) was added 2M aqueous sodium carbonate solution (4.5 ml), and the mixture was stirred at 100° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=4/3) to give the title compound (0.44 g, yield 53%).
  • 1H-NMR (CDCl3) δ: 1.76 (1H, t, J=5.8 Hz), 2.37 (3H, d, J=1.9 Hz), 4.21 (3H, s), 4.78 (2H, d, J=5.8 Hz), 7.33 (1H, t, J=7.9 Hz), 7.47 (1H, dd, J=8.1, 2.2 Hz), 7.54 (1H, d, J=8.1 Hz), 8.02 (1H, d, J=2.2 Hz), 8.23 (1H, dd, J=10.7, 1.6 Hz), 8.29 (1H, dd, J=7.9, 1.6 Hz).
    • (3) tert-butyl N-{4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate
  • Figure US20200087266A1-20200319-C00123
  • Under an argon atmosphere, to a solution of {4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.44 g, 1.2 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (13 ml) were added triethylamine (0.22 ml, 1.6 mmol) and methanesulfonyl chloride (0.10 ml, 1.3 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was added to a solution of di-tert-butyl iminodicarboxylate (0.32 g, 1.5 mmol) and cesium carbonate (1.2 g, 3.6 mmol) in N,N-dimethylformamide (3.0 ml) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=6/1) to give the title compound (0.64 g, yield 94%).
  • 1H-NMR (CDCl3) δ: 1.48 (18H, s), 2.37 (3H, d, J=1.6 Hz), 4.19 (3H, s), 4.83 (2H, s), 7.31 (1H, t, J=7.9 Hz), 7.40 (1H, dd, J=8.4, 2.3 Hz), 7.49 (1H, d, J=8.4 Hz), 8.00 (1H, d, J=2.3 Hz), 8.22 (1H, dd, J=10.7, 1.6 Hz), 8.28 (1H, dd, J=7.9, 1.6 Hz).
    • (4) 4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride
  • Figure US20200087266A1-20200319-C00124
  • Under an argon atmosphere, to a solution of tert-butyl N-{4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate (0.64 g, 1.1 mmol) obtained in the above-mentioned (3) in 1,4-dioxane (2.0 ml) was added 4M hydrogen chloride/1,4-dioxane solution (6.0 ml) at room temperature, and the mixture was stirred for 2 hr. To the reaction mixture was added n-hexane (32 ml), and the mixture was stirred for 45 min. The solid was collected by filtration from the suspension, and dried under reduced pressure to give the title compound (0.45 g, yield 99%).
  • 1H-NMR (DMSO-D6) δ: 2.36 (3H, d, J=1.4 Hz), 4.13-4.19 (2H, m), 4.17 (3H, s), 7.55 (1H, t, J=8.0 Hz), 7.71 (1H, dd, J=8.1, 2.1 Hz), 7.75 (1H, d, J=8.1 Hz), 8.16-8.20 (2H, m), 8.27 (1H, dd, J=7.9, 1.6 Hz), 8.38 (3H, br s).
    • (5) N-{4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00125
  • Under an argon atmosphere, to a solution of 4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.070 g, 0.18 mmol) obtained in the above-mentioned (4), HOBt.H2O (0.041 g, 0.27 mmol) and 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.042 g, 0.27 mmol) in N,N-dimethylformamide (1.0 ml) were added WSC.HCl (0.051 g, 0.27 mmol) and triethylamine (0.037 ml, 0.027 mmol) at room temperature, and the mixture was stirred for 1.5 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=2/1) to give the title compound (0.080 g, yield 90%).
  • 1H-NMR (CDCl3) δ: 1.45 (6H, s), 2.37 (3H, d, J=1.9 Hz), 4.20 (3H, s), 4.55 (2H, d, J=5.8 Hz), 6.23 (1H, br s), 7.30-7.37 (2H, m), 7.52 (1H, d, J=8.4 Hz), 7.93 (1H, d, J=2.3 Hz), 8.22 (1H, dd, J=10.7, 1.6 Hz), 8.28 (1H, dd, J=7.9, 1.6 Hz).
    • (6) N-{4-chloro-3-[4-(3-fluoro-4-methyl-phenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-81)
  • Figure US20200087266A1-20200319-C00126
  • Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.077 g, 0.16 mmol) obtained in the above-mentioned (5) in methanol (1.4 ml) was added 4M aqueous sodium hydroxide solution (0.23 ml) at room temperature, and the mixture was stirred at 60° C. for 2 hr. To the reaction mixture were added 10% aqueous citric acid solution (0.070 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.070 g, yield 92%).
    • Production Example 11 Synthesis of N-{4-chloro-3-[4-hydroxy-6-(4-isopropylphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-98)
  • Figure US20200087266A1-20200319-C00127
    • (1) 2-chloro-4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00128
  • Under an argon atmosphere, to a suspension of 4-isopropylphenylboronic acid (0.30 g, 1.7 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (0.23 g, 1.4 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.11 g, 0.14 mmol) in 1,4-dioxane (4.0 ml) was added 2M aqueous sodium carbonate solution (2.0 ml) at room temperature, and the mixture was stirred at 100° C. for 1.5 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=20/1) to give the title compound (0.21 g, yield 57%).
  • 1H-NMR (CDCl3) δ: 1.29 (6H, d, J=7.1 Hz), 2.99-3.02 (1H, m), 4.16 (3H, s), 7.34-7.38 (2H, m), 8.39-8.43 (2H, m).
    • (2) {4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol
  • Figure US20200087266A1-20200319-C00129
  • Under an argon atmosphere, to a suspension of 2-chloro-4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazine (0.21 g) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.15 g, 0.80 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.066 g, 0.080 mmol) in 1,4-dioxane (2.4 ml) was added 2M aqueous sodium carbonate solution (1.2 ml) at room temperature, and the mixture was stirred at 100° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=5/3) to give the title compound (0.15 g, yield 51%).
  • 1H-NMR (CDCl3) δ: 1.30 (6H, d, J=7.1 Hz), 1.77 (1H, t, J=6.1 Hz), 2.95-3.07 (1H, m), 4.20 (3H, s), 4.77 (2H, d, J=6.1 Hz), 7.35-7.39 (2H, m), 7.46 (1H, dd, J=8.2, 2.2 Hz), 7.54 (1H, d, J=8.2 Hz), 8.01 (1H, dd, J=2.2, 0.4 Hz), 8.50-8.54 (2H, m).
    • (3) tert-butyl N-{4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate
  • Figure US20200087266A1-20200319-C00130
  • Under an argon atmosphere, to a solution of {4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.15 g, 0.41 mmol) obtained in the above-mentioned (2) and triphenylphosphine (0.16 g, 0.62 mmol) in chloroform (1.5 ml) was added carbon tetrabromide (0.20 g, 0.62 mmol) under ice-cooling, and the mixture was stirred at room temperature for 10 min. The reaction mixture was applied to silica gel column chromatography (eluent: n-hexane/ethyl acetate=30/1−10/1) and concentrated under reduced pressure.
  • A solution of the residue in N,N-dimethylformamide (1.5 ml) was added to a solution of di-tert-butyl iminodicarboxylate (0.089 g, 0.41 mmol) and sodium hydride (0.016 g, 60 wt % oil dispersion) in N,N-dimethylformamide (0.70 ml) under ice-cooling, and the mixture was stirred at room temperature for 15 min. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=5/1) to give the title compound (0.20 g, yield 85%).
  • 1H-NMR (CDCl3) δ: 1.30 (6H, d, J=7.0 Hz), 1.47 (18H, s), 2.94-3.05 (1H, m), 4.19 (3H, s), 4.83 (2H, s), 7.34-7.41 (3H, m), 7.48 (1H, d, J=8.4 Hz), 8.00 (1H, d, J=2.3 Hz), 8.49-8.53 (2H, m).
    • (4) 4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride
  • Figure US20200087266A1-20200319-C00131
  • Under an argon atmosphere, to tert-butyl N-{4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate (0.20 g, 0.35 mmol) obtained in the above-mentioned (3) was added 4M hydrogen chloride/1,4-dioxane solution (2.0 ml) at room temperature, and the mixture was stirred for 1 hr. The suspension was concentrated under reduced pressure, and azeotropically distilled with ethyl acetate (twice) to give the title compound as a crude product (0.14 g).
    • (5) N-{4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00132
  • Under an argon atmosphere, to a solution of a crude product (0.10 g) of 4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride obtained in the above-mentioned (4), HOBt.H2O (0.052 g, 0.34 mmol) and WSC.HCl (0.066 g, 0.34 mmol) in N,N-dimethylformamide (1.0 ml) were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.042 g, 0.27 mmol) and triethylamine (0.069 ml, 0.49 mmol) at room temperature, and the mixture was stirred for 4 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=1/1) to give the title compound (0.054 g).
  • 1H-NMR (CDCl3) δ: 1.30 (6H, d, J=6.8 Hz), 1.44 (6H, s), 2.95-3.05 (1H, m), 4.18 (3H, s), 4.53 (2H, d, J=5.7 Hz), 6.34 (1H, br s), 7.30-7.39 (3H, m), 7.50 (1H, d, J=8.4 Hz), 7.91 (1H, d, J=2.2 Hz), 8.49-8.53 (2H, m).
    • (6) N-{4-chloro-3-[4-hydroxy-6-(4-isopropylphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-98)
  • Figure US20200087266A1-20200319-C00133
  • Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.050 g, 0.099 mmol) obtained in the above-mentioned (5) in methanol (0.50 ml) was added 4M aqueous sodium hydroxide solution (0.20 ml) at room temperature, and the mixture was stirred at 60° C. for 2 hr. To the reaction mixture were added 2N hydrochloric acid (0.40 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.043 g, yield 89%).
    • Production Example 12 Synthesis of N-{4-chloro-3-[4-hydroxy-6-(4-isobutoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-109)
  • Figure US20200087266A1-20200319-C00134
    • (1) 2-chloro-4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00135
  • Under an argon atmosphere, to a suspension of 4-isobutoxyphenylboronic acid (0.50 g, 2.58 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (0.93 g, 5.15 mmol), tetrakis(triphenylphosphine)palladium(0) (0.15 g, 0.129 mmol) and sodium carbonate (0.819 g, 7.73 mmol) in toluene (5.0 ml) was added distilled water (3.5 ml), and the mixture was stirred at 86° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=10/1) to give the title compound (0.606 g, yield 80%).
  • 1H-NMR (CDCl3) δ: 1.05 (6H, d, J=6.7 Hz), 2.07-2.17 (1H, m), 3.81 (2H, d, J=6.5 Hz), 4.14 (3H, s), 6.95-7.00 (2H, m), 8.42-8.46 (2H, m).
    • (2) {4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol
  • Figure US20200087266A1-20200319-C00136
  • Under an argon atmosphere, a suspension of 2-chloro-4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazine (0.60 g, 2.0 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.57 g, 3.1 mmol), [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.083 g, 0.10 mmol) and tripotassium phosphate (1.3 g, 6.1 mmol) in N,N-dimethylformamide (6.0 ml) was stirred at 60° C. for 1.5 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water and partitioned, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=3/2) to give the title compound (0.32 g, yield 39%).
  • 1H-NMR (CDCl3) δ: 1.05 (6H, d, J=6.7 Hz), 1.77 (1H, t, J=5.9 Hz), 2.08-2.18 (1H, m), 3.82 (2H, d, J=6.5 Hz), 4.19 (3H, s), 4.77 (2H, d, J=5.9 Hz), 6.98-7.01 (2H, m), 7.46 (1H, dd, J=8.2, 2.2 Hz), 7.53 (1H, d, J=8.2 Hz), 8.00 (1H, d, J=2.2 Hz), 8.55 (2H, m).
    • (3) tert-butyl N-{4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate
  • Figure US20200087266A1-20200319-C00137
  • Under an argon atmosphere, to a solution of {4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.24 g, 0.61 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (2.0 ml) were added triethylamine (0.11 ml, 0.79 mmol) and methanesulfonyl chloride (0.052 ml, 0.67 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (1.5 ml) were added cesium carbonate (0.59 g, 1.8 mmol) and di-tert-butyl iminodicarboxylate (0.16 g, 0.73 mmol) at room temperature, and the mixture was stirred for 1.5 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=6/1) to give the title compound (0.34 g, yield 92%).
  • 1H-NMR (CDCl3) δ: 1.05 (6H, d, J=6.7 Hz), 1.47 (18H, s), 2.08-2.18 (1H, m), 3.82 (2H, d, J=6.5 Hz), 4.18 (3H, s), 4.82 (2H, s), 6.96-7.00 (2H, m), 7.39 (1H, dd, J=8.3, 2.3 Hz), 7.48 (1H, d, J=8.3 Hz), 7.99 (1H, d, J=2.3 Hz), 8.52-8.56 (2H, m).
    • (4) 4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride
  • Figure US20200087266A1-20200319-C00138
  • Under an argon atmosphere, to a solution of tert-butyl N-{4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate (0.34 g, 0.56 mmol) obtained in the above-mentioned (3) in 1,4-dioxane (1.0 ml) was added 4M hydrogen chloride/1,4-dioxane solution (3.0 ml) at room temperature, and the mixture was stirred for 2.5 hr. To the reaction mixture was added n-hexane (20 ml), and the mixture was stirred. The solid was collected by filtration from the suspension, and dried under reduced pressure to give the title compound (0.24 g, yield 95%).
  • 1H-NMR (DMSO-D6) δ: 1.01 (6H, d, J=6.8 Hz), 2.01-2.11 (1H, m), 3.88 (2H, d, J=6.4 Hz), 4.14 (3H, s), 4.12-4.17 (2H, m), 7.12-7.15 (2H, m), 7.72 (2H, br s), 8.13 (1H, br s), 8.40-8.51 (5H, m).
    • (5) N-{4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00139
  • Under an argon atmosphere, to a solution of 4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.065 g, 0.14 mmol) obtained in the above-mentioned (4), HOBt.H2O (0.033 g, 0.22 mmol) and 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.034 g, 0.22 mmol) in N,N-dimethylformamide (0.70 ml) were added WSC.HCl (0.042 g, 0.22 mmol) and triethylamine (0.030 ml, 0.22 mmol) at room temperature, and the mixture was stirred for 5 hr. To the reaction mixture were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.034 g, 0.22 mmol), WSC.HCl (0.042 g, 0.22 mmol), HOBt.H2O (0.033 g, 0.22 mmol) and triethylamine (0.030 ml, 0.22 mmol), and the mixture was stirred for 1 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=3/1) to give the title compound (0.068 g, yield 86%).
  • 1H-NMR (CDCl3) δ: 1.06 (6H, d, J=6.8 Hz), 1.44 (6H, br s), 2.08-2.18 (1H, m), 3.82 (2H, d, J=6.6 Hz), 4.19 (3H, s), 4.55 (2H, d, J=5.7 Hz), 6.21 (1H, br s), 6.97-7.01 (2H, m), 7.34 (1H, dd, J=8.3, 2.3 Hz), 7.51 (1H, d, J=8.3 Hz), 7.91 (1H, d, J=2.3 Hz), 8.53-8.55 (2H, m).
    • (6) N-{4-chloro-3-[4-hydroxy-6-(4-isobutoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-109)
  • Figure US20200087266A1-20200319-C00140
  • Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.066 g, 0.12 mmol) obtained in the above-mentioned (5) in methanol (1.1 ml) was added 4M aqueous sodium hydroxide solution (0.18 ml) at room temperature, and the mixture was stirred at 60° C. for 2 hr. To the reaction mixture were added 10% aqueous citric acid solution (0.55 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.057 g, yield 88%). A suspension of the title compound (0.030 g) in acetonitrile (0.60 ml) was stirred at room temperature, and the solid was collected by filtration and dried to give the title compound as crystals (0.011 g).
    • Production Example 13 Synthesis of N-{4-chloro-3-[4-hydroxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-122)
  • Figure US20200087266A1-20200319-C00141
    • (1) 2-chloro-4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00142
  • Under an argon atmosphere, to a suspension of 4-propoxyphenylboronic acid (1.0 g, 5.6 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (2.0 g, 11 mmol) and tetrakis(triphenylphosphine)palladium(0) (0.65 g, 0.56 mmol) in toluene (25 ml) was added 2M aqueous sodium carbonate solution (8.4 ml), and the mixture was stirred at 100° C. for 1 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=20/1) to give the title compound (1.1 g, yield 70%).
  • 1H-NMR (CDCl3) δ: 1.06 (3H, t, J=7.4 Hz), 1.83-1.87 (2H, m), 4.02 (2H, t, J=6.6 Hz), 4.14 (3H, s), 6.96-6.99 (2H, m), 8.43-8.45 (2H, m).
    • (2) {4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]phenyl}methanol
  • Figure US20200087266A1-20200319-C00143
  • Under an argon atmosphere, to a solution of 2-chloro-4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazine (0.75 g, 2.7 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.60 g, 3.2 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.22 g, 0.27 mmol) in 1,4-dioxane (15 ml) was added 2M aqueous sodium carbonate solution (5.4 ml), and the mixture was stirred at 100° C. for 3 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water and partitioned, washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: chloroform/ethyl acetate=10/1) to give the title compound (0.95 g, yield 91%).
  • 1H-NMR (CDCl3) δ: 1.07 (3H, t, J=7.4 Hz), 1.77 (1H, t, J=5.8 Hz), 1.84-1.87 (2H, m), 4.02 (2H, t, J=6.6 Hz), 4.19 (3H, s), 4.77 (2H, d, J=5.8 Hz), 7.00 (2H, d, J=8.7 Hz), 7.45 (1H, dd, J=8.3, 1.9 Hz), 7.53 (1H, d, J=8.3 Hz), 8.00 (1H, d, J=1.9 Hz), 8.55 (2H, d, J=8.7 Hz).
    • (3) tert-butyl N-{4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate
  • Figure US20200087266A1-20200319-C00144
  • Under an argon atmosphere, to a solution of {4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]phenyl}methanol (0.95 g, 2.5 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (13 ml) were added triethylamine (0.45 ml, 3.2 mmol) and methanesulfonyl chloride (0.23 ml, 3.0 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (13 ml) were added cesium carbonate (2.4 g, 7.4 mmol) and di-tert-butyl iminodicarboxylate (0.64 g, 3.0 mmol) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=10/1) to give the title compound (1.3 g, yield 90%).
    • (4) 4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzylamine hydrochloride
  • Figure US20200087266A1-20200319-C00145
  • Under an argon atmosphere, to tert-butyl N-{4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate (1.3 g, 2.2 mmol) obtained in the above-mentioned (3) was added 4M hydrogen chloride/1,4-dioxane solution (5.0 ml) at room temperature, and the mixture was stirred for 30 min. To the reaction mixture were added 1,4-dioxane (2.0 ml) and n-hexane (5.0 ml), and the mixture was stirred for 45 min. The solid was collected by filtration from the suspension, and dried under reduced pressure to give the title compound (0.68 g, yield 73%).
  • 1H-NMR (DMSO-D6) δ: 1.00 (3H, t, J=7.4 Hz), 1.73-1.83 (2H, m), 4.06 (2H, t, J=6.5 Hz), 4.12-4.18 (2H, m), 4.14 (3H, s), 7.12-7.16 (2H, m), 7.69-7.74 (2H, m), 8.13 (1H, br s), 8.44 (3H, br s), 8.45-8.50 (2H, m).
    • (5) N-{4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00146
  • Under an argon atmosphere, to a solution of 4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.10 g, 0.24 mmol) obtained in the above-mentioned (4), HOBt.H2O (0.054 g, 0.36 mmol) and WSC.HCl (0.068 g, 0.36 mmol) in N,N-dimethylformamide (1.5 ml) were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.056 g, 0.36 mmol) and triethylamine (0.099 ml, 0.71 mmol) at room temperature, and the mixture was stirred for 4 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=10/3) to give the title compound (0.096 g, yield 78%).
  • 1H-NMR (DMSO-D6) δ: 1.00 (3H, t, J=7.3 Hz), 1.39 (6H, s), 1.73-1.83 (2H, m), 4.05 (2H, t, J=6.5 Hz), 4.11 (3H, s), 4.39 (2H, d, J=5.9 Hz), 7.10-7.14 (2H, m), 7.44 (1H, dd, J=8.1, 2.3 Hz), 7.61 (1H, d, J=8.1 Hz), 7.86 (1H, d, J=2.3 Hz), 8.42-8.47 (2H, m), 8.66 (1H, t, J=5.9 Hz).
    • (6) N-{4-chloro-3-[4-hydroxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-122)
  • Figure US20200087266A1-20200319-C00147
  • Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.094 g, 0.18 mmol) obtained in the above-mentioned (5) in methanol (0.94 ml) was added 4M aqueous sodium hydroxide solution (0.27 ml) at room temperature, and the mixture was stirred at 65° C. for 2 hr. To the reaction mixture were added 2N hydrochloric acid (0.54 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.069 g, yield 75%). A suspension of the title compound (0.050 g) in acetone (1.0 ml) was dissolved by heating under reflux, and the solid was collected by filtration at room temperature and dried to give the title compound as crystals (0.012 g).
    • Production Example 14 Synthesis of N-(4-chloro-3-{4-hydroxy-6-[4-(1-methylcyclopropylmethoxy)phenyl]-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-128)
  • Figure US20200087266A1-20200319-C00148
    • (1) 2-chloro-4-methoxy-6-(4-methoxymethoxyphenyl)-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00149
  • Under an argon atmosphere, to a suspension of 4-(methoxymethoxy)phenylboronic acid (1.0 g, 5.5 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (2.0 g, 11 mmol) and tetrakis(triphenylphosphine)palladium(0) (0.64 g, 0.55 mmol) in toluene (25 ml) was added 2M aqueous sodium carbonate solution (8.3 ml), and the mixture was stirred at 100° C. for 1 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=20/1−10/1) to give the title compound (1.3 g, yield 84%).
    • (2) {4-chloro-3-[4-methoxy-6-(4-methoxymethoxyphenyl)-1,3,5-triazin-2-yl]phenyl}methanol
  • Figure US20200087266A1-20200319-C00150
  • Under an argon atmosphere, to a solution of 2-chloro-4-methoxy-6-(4-methoxymethoxyphenyl)-1,3,5-triazine (1.3 g, 4.4 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.99 g, 5.3 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.36 g, 0.44 mmol) in 1,4-dioxane (25 ml) was added 2M aqueous sodium carbonate solution (8.8 ml), and the mixture was stirred at 100° C. for 3 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water, washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: chloroform/ethyl acetate=10/1) to give the title compound (0.98 g, yield 56%).
    • (3) tert-butyl N-{4-chloro-3-[4-methoxy-6-(4-methoxymethoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate
  • Figure US20200087266A1-20200319-C00151
  • Under an argon atmosphere, to a solution of {4-chloro-3-[4-methoxy-6-(4-methoxymethoxyphenyl)-1,3,5-triazin-2-yl]phenyl}methanol (0.78 g, 2.0 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (7.8 ml) were added triethylamine (0.36 ml, 2.6 mmol) and methanesulfonyl chloride (0.19 ml, 2.4 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (10 ml) were added cesium carbonate (2.0 g, 6.0 mmol) and di-tert-butyl iminodicarboxylate (0.53 g, 2.4 mmol), and the mixture was stirred for 2 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=10/1) to give the title compound (0.80 g, yield 68%).
  • 1H-NMR (DMSO-D6) δ: 1.42 (18H, s), 3.41 (3H, s), 4.12 (3H, s), 4.77 (2H, s), 5.32 (2H, s), 7.18-7.23 (2H, m), 7.45 (1H, dd, J=8.2, 2.3 Hz), 7.65 (1H, d, J=8.2 Hz), 7.91 (1H, d, J=2.3 Hz), 8.43-8.47 (2H, m).
    • (4) 4-[4-(5-aminomethyl-2-chlorophenyl)-6-methoxy-1,3,5-triazin-2-yl]phenol hydrochloride
  • Figure US20200087266A1-20200319-C00152
  • Under an argon atmosphere, to tert-butyl N-{4-chloro-3-[4-methoxy-6-(4-methoxymethoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate (0.40 g, 0.68 mmol) obtained in the above-mentioned (3) was added 4M hydrogen chloride/1,4-dioxane solution (2.0 ml) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture was added n-hexane (3.0 ml), and the mixture was stirred for 45 min. The solid was collected by filtration, and dried under reduced pressure to give the title compound as a crude product (0.26 g).
    • (5) 4-(4-{2-chloro-5-[(3,3,3-trifluoro-2,2-dimethylpropionylamino)methyl]phenyl}-6-methoxy-1,3,5-triazin-2-yl)phenyl 3,3,3-trifluoro-2,2-dimethylpropionate
  • Figure US20200087266A1-20200319-C00153
  • Under an argon atmosphere, to a solution of a crude product (0.10 g) of 4-[4-(5-aminomethyl-2-chlorophenyl)-6-methoxy-1,3,5-triazin-2-yl]phenol hydrochloride obtained in the above-mentioned (4), HOBt.H2O (0.061 g, 0.40 mmol) and WSC.HCl (0.076 g, 0.40 mmol) in N,N-dimethylformamide (1.5 ml) were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.066 g, 0.40 mmol) and triethylamine (0.11 ml, 0.79 mmol) at room temperature, and the mixture was stirred for 2 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: chloroform/ethyl acetate=10/1) to give the title compound (0.090 g).
  • 1H-NMR (DMSO-D6) δ: 1.39 (6H, s), 1.59 (6H, s), 4.15 (3H, s), 4.40 (2H, d, J=6.0 Hz), 7.39-7.47 (3H, m), 7.62 (1H, d, J=8.4 Hz), 7.88 (1H, d, J=2.1 Hz), 8.55-8.60 (2H, m), 8.66 (1H, t, J=6.0 Hz).
    • (6) N-{4-chloro-3-[4-(4-hydroxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00154
  • Under an argon atmosphere, to a solution of 4-(4-{2-chloro-5-[(3,3,3-trifluoro-2,2-dimethylpropionylamino)methyl]phenyl}-6-methoxy-1,3,5-triazin-2-yl)phenyl 3,3,3-trifluoro-2,2-dimethylpropionate (0.070 g, 0.15 mmol) obtained in the above-mentioned (5) in methanol (0.70 ml) was added 5M sodium methoxide/methanol solution (0.032 ml) at room temperature, and the mixture was stirred for 1 hr. The reaction mixture was adjusted to pH=2 with 2N hydrochloric acid under ice-cooling. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: chloroform/ethyl acetate=4/1) to give the title compound (0.036 g, yield 51%).
  • 1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 4.10 (3H, s), 4.39 (2H, d, J=6.2 Hz), 6.91-6.95 (2H, m), 7.42 (1H, dd, J=8.3, 2.3 Hz), 7.60 (1H, d, J=8.3 Hz), 7.84 (1H, d, J=2.3 Hz), 8.34-8.39 (2H, m), 8.65 (1H, t, J=6.2 Hz), 10.38 (1H, br s).
    • (7) N-(4-chloro-3-{4-methoxy-6-[4-(1-methylcyclopropylmethoxy)phenyl]-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00155
  • Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-hydroxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.036 g, 0.075 mmol) obtained in the above-mentioned (6), 1-methyl-cyclopropanemethanol (0.0087 ml, 0.090 mmol) and triphenylphosphine (0.024 g, 0.090 mmol) in tetrahydrofuran (0.50 ml) was added 1.9M diethyl azodicarboxylate/toluene solution (0.051 ml, 0.098 mmol) under ice-cooling, and the mixture was stirred for 1 hr. The reaction mixture was stirred at room temperature for 1 hr, and 1.9M diethyl azodicarboxylate/toluene solution (0.028 ml, 0.053 mmol) was added. The reaction mixture was stirred at room temperature for 1 hr, and purified by preparative thin layer chromatography (eluent: chloroform/ethyl acetate=19/1) to give the title compound (0.029 g, yield 70%).
  • 1H-NMR (DMSO-D6) δ: 0.42 (2H, dd, J=5.6, 4.0 Hz), 0.56 (2H, dd, J=5.4, 4.2 Hz), 1.20 (3H, s), 1.39 (6H, s), 3.88 (2H, s), 4.11 (3H, s), 4.39 (2H, d, J=5.9 Hz), 7.09-7.14 (2H, m), 7.43 (1H, dd, J=8.2, 2.1 Hz), 7.60 (1H, d, J=8.2 Hz), 7.85 (1H, d, J=2.1 Hz), 8.41-8.46 (2H, m), 8.66 (1H, t, J=5.9 Hz).
    • (8) N-(4-chloro-3-{4-hydroxy-6-[4-(1-methylcyclopropylmethoxy)phenyl]-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-128)
  • Figure US20200087266A1-20200319-C00156
  • Under an argon atmosphere, to a solution of N-(4-chloro-3-{4-methoxy-6-[4-(1-methylcyclopropylmethoxy)phenyl]-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (0.028 g, 0.051 mmol) obtained in the above-mentioned (7) in methanol (0.28 ml) was added 4M aqueous sodium hydroxide solution (0.077 ml) at room temperature, and the mixture was stirred at 60° C. for 1 hr. To the reaction mixture were added 2N hydrochloric acid (0.16 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.019 g, yield 69%).
    • Production Example 15 Synthesis of N-{4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-129)
  • Figure US20200087266A1-20200319-C00157
    • (1) 2-chloro-4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00158
  • Under an argon atmosphere, to a suspension of 3-chloro-4-methylphenylboronic acid (0.47 g, 2.8 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (1.0 g, 5.6 mmol) and tetrakis(triphenylphosphine)palladium(0) (0.32 g, 0.28 mmol) in toluene (5.0 ml) was added 2M aqueous sodium carbonate solution (4.2 ml), and the mixture was stirred at 100° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=97/3−94/6) to give the title compound (0.61 g, yield 81%).
  • 1H-NMR (CDCl3) δ: 2.47 (3H, s), 4.17 (3H, s), 7.37 (1H, d, J=8.0 Hz), 8.28 (1H, dd, J=8.0, 1.8 Hz), 8.47 (1H, d, J=1.8 Hz).
    • (2){4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol
  • Figure US20200087266A1-20200319-C00159
  • Under an argon atmosphere, to a solution of 2-chloro-4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazine (0.61 g, 2.3 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.51 g, 2.7 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.19 g, 0.23 mmol) in 1,4-dioxane (6.0 ml) was added 2M aqueous sodium carbonate solution (4.5 ml), and the mixture was stirred at 100° C. for 1.5 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=8/2−6/4) to give the title compound (0.61 g, yield 71%).
  • 1H-NMR (CDCl3) δ: 1.81 (1H, t, J=5.9 Hz), 2.47 (3H, s), 4.21 (3H, s), 4.78 (2H, d, J=5.9 Hz), 7.37 (1H, d, J=7.9 Hz), 7.47 (1H, dd, J=8.1, 2.2 Hz), 7.54 (1H, d, J=8.1 Hz), 8.01 (1H, d, J=2.2 Hz), 8.38 (1H, dd, J=7.9, 1.8 Hz), 8.57 (1H, d, J=1.8 Hz).
    • (3) 4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride
  • Figure US20200087266A1-20200319-C00160
  • Under an argon atmosphere, to a solution of {4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.61 g, 1.6 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (6.0 ml) were added triethylamine (0.29 ml, 2.1 mmol) and methanesulfonyl chloride (0.15 ml, 1.9 mmol) under ice-cooling, and the mixture was stirred for 1 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (6.0 ml) were added cesium carbonate (1.6 g, 4.8 mmol) and di-tert-butyl iminodicarboxylate (0.42 g, 1.9 mmol) at room temperature, and the mixture was stirred for 2 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=95/5−80/20). Under an argon atmosphere, to a solution of the purified product in 1,4-dioxane (2.0 ml) was added 4M hydrogen chloride/1,4-dioxane solution (8.0 ml) at room temperature, and the mixture was stirred for 2.5 hr. To the reaction mixture was added n-hexane, and the solid was collected by filtration, and dried under reduced pressure to give the title compound (0.67 g, yield 99%).
  • 1H-NMR (DMSO-D6) δ: 2.46 (3H, s), 4.12-4.21 (5H, m), 7.62 (1H, d, J=8.0 Hz), 7.73-7.75 (2H, m), 8.17 (1H, br s), 8.38 (1H, dd, J=8.0, 1.6 Hz), 8.47 (1H, d, J=1.6 Hz), 8.48 (3H, br s).
    • (4)N-{4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00161
  • Under an argon atmosphere, to a solution of 4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.070 g, 0.17 mmol) obtained in the above-mentioned (3), HOBt.H2O (0.039 g, 0.26 mmol) and WSC.HCl (0.049 g, 0.26 mmol) in N,N-dimethylformamide (0.70 ml) were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.037 g, 0.24 mmol) and triethylamine (0.071 ml, 0.51 mmol) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=9/1−8/2) to give the title compound (0.072 g, yield 82%).
  • 1H-NMR (CDCl3) δ: 1.45 (6H, s), 2.47 (3H, s), 4.21 (3H, s), 4.56 (2H, d, J=5.6 Hz), 6.24 (1H, br s), 7.34-7.39 (2H, m), 7.52 (1H, d, J=8.2 Hz), 7.92 (1H, d, J=2.3 Hz), 8.38 (1H, dd, J=8.2, 1.8 Hz), 8.56 (1H, d, J=1.8 Hz).
    • (5)N-{4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-129)
  • Figure US20200087266A1-20200319-C00162
  • Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.072 g, 0.14 mmol) obtained in the above-mentioned (4) in methanol (0.70 ml) was added 4M aqueous sodium hydroxide solution (0.28 ml) at room temperature, and the mixture was stirred at 60° C. for 1 hr. To the reaction mixture were added 2N hydrochloric acid (0.56 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.057 g, yield 82%).
    • Production Example 16 Synthesis of N-{4-chloro-3-[4-hydroxy-6-(3-isopropyl-4-trifluoromethylphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-130)
  • Figure US20200087266A1-20200319-C00163
    • (1) 4-benzyloxy-2-bromo-1-trifluoromethylbenzene
  • Figure US20200087266A1-20200319-C00164
  • Under an argon atmosphere, to a solution of 2-bromo-4-fluoro-1-trifluoromethylbenzene (1.5 g, 6.2 mmol) and sodium hydride (0.74 g, 60 wt % oil dispersion) in N,N-dimethylformamide (15 ml) was added benzyl alcohol (0.64 ml, 6.2 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was stirred at 60° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=99/1−98/2) to give the title compound (1.3 g, yield 69%).
  • 1H-NMR (CDCl3) δ: 5.08 (2H, s), 6.93 (1H, dd, J=8.8, 2.4 Hz), 7.30 (1H, d, J=2.4 Hz), 7.33-7.41 (5H, m), 7.57 (1H, d, J=8.8 Hz).
    • (2) 4-benzyloxy-2-isopropenyl-1-trifluoromethylbenzene
  • Figure US20200087266A1-20200319-C00165
  • Under an argon atmosphere, to a solution of 4-benzyloxy-2-bromo-1-trifluoromethylbenzene (1.3 g, 3.9 mmol) obtained in the above-mentioned (1) in 1,4-dioxane (13 ml) were added 2-isopropenyl-4,4,5,5-tetramethyl-[1,3,2]dioxaborolane (0.99 g, 5.9 mmol), [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.32 g, 0.39 mmol) and 2M aqueous sodium carbonate solution (5.9 ml) at room temperature, and the mixture was stirred at 100° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=99/1−97/3) to give the title compound (1.1 g, yield 99%).
  • 1H-NMR (CDCl3) δ: 2.04 (3H, s), 4.88 (1H, br s), 5.08 (2H, s), 5.18 (1H, br s), 6.82 (1H, d, J=2.6 Hz), 6.89 (1H, dd, J=8.8, 2.6 Hz), 7.31-7.42 (5H, m), 7.54 (1H, d, J=8.8 Hz).
    • (3) 3-isopropyl-4-trifluoromethylphenol
  • Figure US20200087266A1-20200319-C00166
  • Under an argon atmosphere, to a solution of 4-benzyloxy-2-isopropenyl-1-trifluoromethylbenzene (1.2 g, 3.9 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (12 ml) was added 10 wt % palladium carbon (0.23 g) at room temperature, and the mixture was stirred under 1 atm hydrogen atmosphere for 5 hr. Under a nitrogen atmosphere, the reaction mixture was filtered through celite and eluted with ethyl acetate. The filtrate was concentrated under reduced pressure to give the title compound (0.76 g, yield 96%).
  • 1H-NMR (CDCl3) δ: 1.23 (6H, d, J=6.7 Hz), 3.24-3.35 (1H, m), 5.04 (1H, br s), 6.66 (1H, dd, J=8.6, 2.6 Hz), 6.87 (1H, d, J=2.6 Hz), 7.46 (1H, d, J=8.6 Hz).
    • (4) 3-isopropyl-4-trifluoromethylphenyl trifluoromethanesulfonate
  • Figure US20200087266A1-20200319-C00167
  • Under an argon atmosphere, to a solution of 3-isopropyl-4-trifluoromethylphenol (0.77 g, 3.8 mmol) obtained in the above-mentioned (3) in chloroform (8.0 ml) were added triethylamine (0.58 ml, 4.1 mmol) and trifluoromethanesulfonic anhydride (0.67 ml, 4.0 mmol) under ice-cooling, and the mixture was stirred for 1 hr. To the reaction mixture were added water and chloroform, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=98/2) to give the title compound (0.78 g, yield 62%).
  • 1H-NMR (CDCl3) δ: 1.28 (6H, d, J=6.7 Hz), 3.34-3.46 (1H, m), 7.19 (1H, dd, J=8.8, 2.4 Hz), 7.34 (1H, d, J=2.4 Hz), 7.70 (1H, d, J=8.8 Hz).
    • (5) 2-(3-isopropyl-4-trifluoromethylphenyl)-4,4,5,5-tetramethyl-[1,3,2]dioxaborolane
  • Figure US20200087266A1-20200319-C00168
  • Under an argon atmosphere, to a solution of 3-isopropyl-4-trifluoromethylphenyl trifluoromethanesulfonate (0.78 g, 2.3 mmol) obtained in the above-mentioned (4) in DMSO (8.0 ml) were added bis(pinacolato)diboron (0.71 g, 2.8 mmol), potassium acetate (0.68 g, 7.0 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.095 g, 0.12 mmol) at room temperature, and the mixture was stirred at 80° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=98/2) to give the title compound (0.48 g, yield 66%).
  • 1H-NMR (CDCl3) δ: 1.29 (6H, d, J=7.0 Hz), 1.36 (12H, s), 3.29-3.40 (1H, m), 7.57 (1H, d, J=7.9 Hz), 7.68 (1H, d, J=7.9 Hz), 7.88 (1H, br s).
    • (6) 2-chloro-4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00169
  • Under an argon atmosphere, to a suspension of 2-(3-isopropyl-4-trifluoromethylphenyl)-4,4,5,5-tetramethyl-[1,3,2]dioxaborolane (0.48 g, 1.5 mmol) obtained in the above-mentioned (5), 2,4-dichloro-6-methoxy-1,3,5-triazine (0.69 g, 3.8 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.13 g, 0.15 mmol) in 1,4-dioxane (5.0 ml) was added 2M aqueous sodium carbonate solution (3.1 mL), and the mixture was stirred at 100° C. for 1 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=97/3−94/6) to give the title compound (0.36 g, yield 71%).
    • (7) {4-chloro-3-[4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol
  • Figure US20200087266A1-20200319-C00170
  • Under an argon atmosphere, to a solution of 2-chloro-4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazine (0.36 g, 1.1 mmol) obtained in the above-mentioned (6), 2-chloro-5-hydroxymethylphenylboronic acid (0.25 g, 1.3 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.089 g, 0.11 mmol) in 1,4-dioxane (3.6 ml) was added 2M aqueous sodium carbonate solution (2.2 ml), and the mixture was stirred at 100° C. for 1.5 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=8/2−1/1) to give the title compound (0.30 g, yield 62%).
  • 1H-NMR (CDCl3) δ: 1.36 (6H, d, J=6.8 Hz), 1.79 (1H, t, J=6.0 Hz), 3.37-3.48 (1H, m), 4.24 (3H, s), 4.79 (2H, d, J=6.0 Hz), 7.49 (1H, dd, J=8.4, 2.2 Hz), 7.57 (1H, d, J=8.4 Hz), 7.75 (1H, d, J=8.4 Hz), 8.07 (1H, d, J=2.2 Hz), 8.47 (1H, d, J=8.4 Hz), 8.73 (1H, br s).
    • (8) 4-chloro-3-[4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride
  • Figure US20200087266A1-20200319-C00171
  • Under an argon atmosphere, to a solution of {4-chloro-3-[4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.30 g, 0.68 mmol) obtained in the above-mentioned (7) in tetrahydrofuran (3.0 ml) were added triethylamine (0.12 ml, 0.89 mmol) and methanesulfonyl chloride (0.063 ml, 0.82 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (3.0 ml) were added cesium carbonate (0.67 g, 2.0 mmol) and di-tert-butyl iminodicarboxylate (0.18 g, 0.82 mmol) at room temperature, and the mixture was stirred for 1.5 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=95/5−80/20). Under an argon atmosphere, to a solution (1.0 ml) of the purified product in 1,4-dioxane was added 4M hydrogen chloride/1,4-dioxane solution (4.0 ml) at room temperature, and the mixture was stirred for 1.5 hr. To the reaction mixture was added n-hexane, and the solid was collected by filtration and dried under reduced pressure to give the title compound (0.24 g, yield 74%).
  • 1H-NMR (DMSO-D6) δ: 1.33 (6H, d, J=6.7 Hz), 3.28-3.40 (1H, m), 4.13-4.22 (5H, m), 7.73 (1H, dd, J=8.2, 2.2 Hz), 7.77 (1H, d, J=8.3 Hz), 7.92 (1H, d, J=8.3 Hz), 8.20 (1H, d, J=2.2 Hz), 8.35 (3H, br s), 8.48 (1H, d, J=8.8 Hz), 8.70 (1H, s).
    • (9) N-{4-chloro-3-[4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00172
  • Under an argon atmosphere, to a solution of 4-chloro-3-[4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.080 g, 0.17 mmol) obtained in the above-mentioned (8), HOBt.H2O (0.039 g, 0.26 mmol) and WSC.HCl (0.049 g, 0.26 mmol) in N,N-dimethylformamide (0.80 ml) were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.037 g, 0.24 mmol) and triethylamine (0.071 ml, 0.51 mmol) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=19/1−8/2) to give the title compound (0.077 g, yield 79%).
  • 1H-NMR (CDCl3) δ: 1.35 (6H, d, J=6.0 Hz), 1.44 (6H, br s), 3.37-3.49 (1H, m), 4.23 (3H, s), 4.56 (2H, d, J=5.8 Hz), 6.25 (1H, br s), 7.37 (1H, dd, J=8.4, 2.3 Hz), 7.54 (1H, d, J=8.4 Hz), 7.74 (1H, d, J=8.4 Hz), 7.96 (1H, d, J=2.3 Hz), 8.46 (1H, d, J=8.4 Hz), 8.72 (1H, br s).
    • (10) N-{4-chloro-3-[4-hydroxy-6-(3-isopropyl-4-trifluoromethylphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-130)
  • Figure US20200087266A1-20200319-C00173
  • Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.077 g, 0.13 mmol) obtained in the above-mentioned (9) in methanol (0.80 ml) was added 4M aqueous sodium hydroxide solution (0.27 ml) at room temperature, and the mixture was stirred at 60° C. for 1 hr. To the reaction mixture were added 2N hydrochloric acid (0.54 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.066 g, yield 88%).
    • Production Example 17 Synthesis of N-{3-[4-(4-butoxyphenyl)-6-hydroxy-1,3,5-triazin-2-yl]-4-chlorobenzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-131)
  • Figure US20200087266A1-20200319-C00174
    • (1) N-{3-[4-(4-butoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]-4-chlorobenzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00175
  • Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-hydroxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.10 g, 0.21 mmol) obtained in the above-mentioned [Production Example 14] (6), n-butanol (0.023 ml, 0.25 mmol) and triphenylphosphine (0.066 g, 0.25 mmol) in tetrahydrofuran (1.0 ml) was added bis(2-methoxyethyl) azodicarboxylate (0.059 g, 0.25 mmol) under ice-cooling, and the mixture was stirred for 1 hr. To the reaction mixture were added n-butanol (0.019 ml, 0.21 mmol), triphenylphosphine (0.055 g, 0.21 mmol) and bis(2-methoxyethyl) azodicarboxylate (0.049 g, 0.21 mmol), and the mixture was stirred at room temperature for 2 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=1/1) to give the title compound (0.096 g, yield 85%).
    • (2) N-{3-[4-(4-butoxyphenyl)-6-hydroxy-1,3,5-triazin-2-yl]-4-chlorobenzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-131)
  • Figure US20200087266A1-20200319-C00176
  • Under an argon atmosphere, to a solution of N-{3-[4-(4-butoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]-4-chlorobenzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.096 g, 0.18 mmol) obtained in the above-mentioned (1) in methanol (0.96 ml) was added 4M aqueous sodium hydroxide solution (0.27 ml) at room temperature, and the mixture was stirred at 65° C. for 2 hr. To the reaction mixture were added 2N hydrochloric acid (0.54 ml) and water and the mixture was stirred at room temperature. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.086 g, yield 93%).
    • Production Example 18 Synthesis of N-{4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-135)
  • Figure US20200087266A1-20200319-C00177
    • (1) 2-chloro-4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00178
  • Under an argon atmosphere, to a suspension of 2-(3-cyclopropyl-4-fluorophenyl)-4,4,5,5-tetramethyl-[1,3,2]dioxaborolane (0.59 g, 2.2 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (0.81 g, 4.5 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.18 g, 0.22 mmol) in 1,4-dioxane (3.0 ml) was added 2M aqueous sodium carbonate solution (3.4 ml), and the mixture was stirred at 100° C. for 1 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=25/1−20/1) to give the title compound as a crude product (0.44 g).
    • (2) {4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol
  • Figure US20200087266A1-20200319-C00179
  • Under an argon atmosphere, to a solution of a crude product (0.44 g) of 2-chloro-4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazine obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.31 g, 1.6 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.11 g, 0.13 mmol) in 1,4-dioxane (5.4 ml) was added 2M aqueous sodium carbonate solution (2.7 ml), and the mixture was stirred at 100° C. for 1 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=5/3) to give the title compound (0.32 g).
  • 1H-NMR (CDCl3) δ: 0.83-0.88 (2H, m), 1.01-1.07 (2H, m), 1.79 (1H, t, J=6.0 Hz), 2.10-2.19 (1H, m), 4.20 (3H, s), 4.77 (2H, d, J=6.0 Hz), 7.13 (1H, t, J=9.2 Hz), 7.47 (1H, d, J=8.1 Hz), 7.54 (1H, d, J=8.1 Hz), 8.01 (1H, br s), 8.20 (1H, d, J=7.6 Hz), 8.38-8.41 (1H, m).
    • (3) tert-butyl N-{4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate
  • Figure US20200087266A1-20200319-C00180
  • Under an argon atmosphere, to a solution of {4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.32 g, 0.82 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (3.3 ml) were added triethylamine (0.15 ml, 1.1 mmol) and methanesulfonyl chloride (0.076 ml, 0.98 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (3.3 ml) were added cesium carbonate (0.80 g, 2.5 mmol) and di-tert-butyl iminodicarboxylate (0.21 g, 0.98 mmol) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=7/1) to give the title compound (0.40 g, yield 83%).
  • 1H-NMR (CDCl3) δ: 0.84-0.88 (2H, m), 1.01-1.07 (2H, m), 1.47 (18H, s), 2.09-2.18 (1H, m), 4.18 (3H, s), 4.83 (2H, s), 7.11 (1H, dd, J=9.7, 8.6 Hz), 7.40 (1H, dd, J=8.3, 2.2 Hz), 7.49 (1H, d, J=8.3 Hz), 8.00 (1H, d, J=2.2 Hz), 8.19 (1H, dd, J=7.5, 2.2 Hz), 8.36-8.41 (1H, m).
    • (4) 4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3, 5-triazin-2-yl]benzylamine hydrochloride
  • Figure US20200087266A1-20200319-C00181
  • Under an argon atmosphere, to tert-butyl N-{4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate(0.40 g, 0.68 mmol) obtained in the above-mentioned (3) was added 4M hydrogen chloride/1,4-dioxane solution (3.3 ml) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture was added ethyl acetate (35 ml), and the mixture was stirred. The solid was collected by filtration and dried under reduced pressure to give the title compound (0.26 g, yield 89%).
  • 1H-NMR (DMSO-D6) δ: 0.78-0.83 (2H, m), 1.05-1.10 (2H, m), 2.10-2.19 (1H, m), 4.16 (3H, s), 4.16 (2H, s), 7.39 (1H, dd, J=9.9, 8.7 Hz), 7.71 (1H, dd, J=8.4, 2.1 Hz), 7.75 (1H, d, J=8.4 Hz), 8.13 (1H, dd, J=7.7, 2.1 Hz), 8.16 (1H, d, J=2.1 Hz), 8.35-8.37 (4H, m).
    • (5) N-{4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00182
  • Under an argon atmosphere, to a solution of 4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.070 g, 0.17 mmol) obtained in the above-mentioned (4), HOBt.H2O (0.033 g, 0.22 mmol) and WSC.HCl (0.041 g, 0.22 mmol) in N,N-dimethylformamide (2.0 ml) were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.034 g, 0.22 mmol) and triethylamine (0.069 ml, 0.48 mmol) at room temperature, and the mixture was stirred for 3 hr. To the reaction mixture were added saturated aqueous sodium hydrogen carbonate and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=5/3) to give the title compound (0.082 g, yield 94%).
  • 1H-NMR (CDCl3) δ: 0.82-0.87 (2H, m), 1.01-1.05 (2H, m), 1.43 (6H, s), 2.10-2.16 (1H, m), 4.18 (3H, s), 4.54 (2H, d, J=5.6 Hz), 6.21 (1H, br s), 7.11 (1H, t, J=9.2 Hz), 7.34 (1H, d, J=8.3 Hz), 7.51 (1H, d, J=8.3 Hz), 7.90 (1H, s), 8.18 (1H, d, J=7.7 Hz), 8.36-8.40 (1H, m).
    • (6) N-{4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-135)
  • Figure US20200087266A1-20200319-C00183
  • Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.082 g, 0.16 mmol) obtained in the above-mentioned (5) in methanol (1.8 ml) was added 4M aqueous sodium hydroxide solution (0.24 ml) at room temperature, and the mixture was stirred at 60° C. for 3 hr. To the reaction mixture were added 10% aqueous citric acid solution (1.0 ml) and water, and the mixture was stirred at room temperature. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.065 g, yield 81%).
    • Production Example 19 Synthesis of (R)—N-{4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide (Example No. 1-136)
  • Figure US20200087266A1-20200319-C00184
    • (1) benzyl (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionate
  • Figure US20200087266A1-20200319-C00185
  • Under an argon atmosphere, to a suspension of (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid (2.2 g, 14 mmol) and potassium carbonate (2.3 g, 16 mmol) in N,N-dimethylformamide (30 ml) was added benzyl bromide (1.8 ml, 15 mmol) at room temperature, and the mixture was stirred for 4 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=6/1) to give the title compound (3.0 g, yield 90%).
  • 1H-NMR (CDCl3) δ: 1.60 (3H, s), 3.78 (1H, s), 5.31 (2H, s), 7.33-7.42 (5H, m).
    • (2) benzyl (R)-3,3,3-trifluoro-2-methoxy-2-methylpropionate
  • Figure US20200087266A1-20200319-C00186
  • Under an argon atmosphere, to a solution of benzyl (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionate (3.4 g, 14 mmol) obtained in the above-mentioned (1) in N,N-dimethylformamide (40 ml) was added sodium hydride (0.60 g, 60 wt % oil dispersion) under ice-cooling, and the mixture was stirred for 1 hr. To the reaction mixture was added methyl iodide (1.3 ml, 20 mmol), and the mixture was stirred at room temperature for 2hr. To the reaction mixture were added saturated aqueous ammonium chloride and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=15/1) to give the title compound (2.8 g, yield 78%).
  • 1H-NMR (CDCl3) δ: 1.59 (3H, s), 3.40 (3H, s), 5.26 (2H, s), 7.31-7.37 (5H, m).
    • (3) (R)-3,3,3-trifluoro-2-methoxy-2-methylpropionic acid
  • Figure US20200087266A1-20200319-C00187
  • Under an argon atmosphere, to a solution of benzyl (R)-3,3,3-trifluoro-2-methoxy-2-methylpropionate (2.8 g, 11 mmol) obtained in the above-mentioned (2) in ethyl acetate (50 ml) was added 10 wt % palladium carbon (0.23 g) at room temperature, and the mixture was stirred under 1 atm hydrogen atmosphere for 5 hr. Under a nitrogen atmosphere, the reaction mixture was filtered through celite and eluted with ethyl acetate. The filtrate was concentrated under reduced pressure to give the title compound (1.4 g, yield 78%).
  • 1H-NMR (CDCl3) δ: 1.68 (3H, s), 3.54 (3H, s).
    • (4) 2-chloro-4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00188
  • Under an argon atmosphere, to a suspension of 4-chloro-3-methylphenylboronic acid (0.47 g, 2.8 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (1.0 g, 5.6 mmol) and tetrakis(triphenylphosphine)palladium(0) (0.32 g, 0.28 mmol) in toluene (5.0 ml) was added 2M aqueous sodium carbonate solution (4.2 ml), and the mixture was stirred at 100° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=98/2−95/5) to give the title compound (0.50 g, yield 48%).
  • 1H-NMR (CDCl3) δ: 2.47 (3H, s), 4.17 (3H, s), 7.47 (1H, d, J=8.4 Hz), 8.26 (1H, dd, J=8.4, 2.1 Hz), 8.36 (1H, d, J=2.1 Hz).
    • (5) {4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol
  • Figure US20200087266A1-20200319-C00189
  • Under an argon atmosphere, to a solution of 2-chloro-4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazine (0.50 g, 1.3 mmol) obtained in the above-mentioned (4), 2-chloro-5-hydroxymethylphenylboronic acid (0.30 g, 1.6 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.11 g, 0.13 mmol) in 1,4-dioxane (5.0 ml) was added 2M aqueous sodium carbonate solution (2.6 ml), and the mixture was stirred at 100° C. for 1.5 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=8/2−6/4) to give the title compound (0.40 g, yield 80%).
  • 1H-NMR (CDCl3) δ: 1.79 (1H, t, J=5.3 Hz), 2.48 (3H, s), 4.21 (3H, s), 4.78 (2H, d, J=5.3 Hz), 7.45-7.50 (2H, m), 7.54 (1H, d, J=8.1 Hz), 8.01 (1H, d, J=2.1 Hz), 8.37 (1H, dd, J=8.4, 2.1 Hz), 8.46 (1H, d, J=2.1 Hz).
    • (6) 4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride
  • Figure US20200087266A1-20200319-C00190
  • Under an argon atmosphere, to a solution of {4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.40 g, 1.1 mmol) obtained in the above-mentioned (5) in tetrahydrofuran (4.0 ml) were added triethylamine (0.19 ml, 1.4 mmol) and methanesulfonyl chloride (0.098 ml, 1.3 mmol) under ice-cooling, and the mixture was stirred for 1 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (2.0 ml) were added cesium carbonate (1.0 g, 3.2 mmol) and di-tert-butyl iminodicarboxylate (0.37 g, 1.7 mmol) at room temperature, and the mixture was stirred for 3 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=95/5−80/20). Under an argon atmosphere, to a solution (2.0 ml) of the purified product in 1,4-dioxane was added 4M hydrogen chloride/1,4-dioxane solution (4.0 ml) at room temperature, and the mixture was stirred for 2 hr. To the reaction mixture was added n-hexane, and the solid was collected by filtration and dried under reduced pressure to give the title compound (0.43 g, yield 99%).
  • 1H-NMR (DMSO-D6) δ: 2.47 (3H, s), 4.13-4.19 (5H, m), 7.67 (1H, d, J=8.3 Hz), 7.71-7.76 (2H, m), 8.16 (1H, d, J=1.6 Hz), 8.35 (1H, dd, J=8.3, 1.6 Hz), 8.41-8.50 (4H, m).
    • (7) (R)—N-{4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide
  • Figure US20200087266A1-20200319-C00191
  • Under an argon atmosphere, to a solution of 4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.070 g, 0.17 mmol) obtained in the above-mentioned (6), HOBt.H2O (0.039 g, 0.26 mmol) and WSC.HCl (0.049 g, 0.26 mmol) in N,N-dimethylformamide (0.70 ml) were added (R)-3,3,3-trifluoro-2-methoxy-2-methylpropionic acid (0.038 g, 0.22 mmol) obtained in the above-mentioned (3) and triethylamine (0.071 ml, 0.51 mmol) at room temperature, and the mixture was stirred for 18 hr. To the reaction mixture were added saturated aqueous sodium hydrogen carbonate and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=3/2) to give the title compound (0.058 g, yield 65%).
  • 1H-NMR (CDCl3) δ: 1.66 (3H, s), 2.48 (3H, s), 3.45 (3H, s), 4.20 (3H, s), 4.48 (1H, dd, J=15.1, 5.8 Hz), 4.63 (1H, dd, J=15.1, 6.5 Hz), 7.16 (1H, br s), 7.37 (1H, dd, J=8.3, 2.3 20 Hz), 7.48 (1H, d, J=8.3 Hz), 7.53 (1H, d, J=8.3 Hz), 7.92 (1H, d, J=2.3 Hz), 8.36 (1H, dd, J=8.3, 2.0 Hz), 8.46 (1H, d, J=2.0 Hz).
    • (8) (R)—N-{4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide (Example No. 1-136)
  • Figure US20200087266A1-20200319-C00192
  • Under an argon atmosphere, to a solution of (R)—N-{4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide (0.058 g, 0.11 mmol) obtained in the above-mentioned (7) in methanol (1.3 ml) was added 4M aqueous sodium hydroxide solution (0.17 ml) at room temperature, and the mixture was stirred at 60° C. for 3 hr. To the reaction mixture were added 10% aqueous citric acid solution (0.68 ml) and water, and the mixture was stirred at room temperature. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.051 g, yield 88%).
    • Production Example 20 Synthesis of (R)—N-{4-chloro-3-[4-hydroxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide (Example No. 1-137)
  • Figure US20200087266A1-20200319-C00193
    • (1) (R)—N-{4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide
  • Figure US20200087266A1-20200319-C00194
  • Under an argon atmosphere, to a solution of 4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.80 g, 0.19 mmol) obtained in [Production Example 13] (4), HOBt.H2O (0.044 g, 0.28 mmol) and WSC.HCl (0.055 g, 0.28 mmol) in N,N-dimethylformamide (1.0 ml) were added (R)-3,3,3-trifluoro-2-methoxy-2-methylpropionic acid (0.046 g, 0.27 mmol) obtained in [Production Example 19] (3) and triethylamine (0.080 ml, 0.57 mmol) at room temperature, and the mixture was stirred for 18 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=8/2−3/2) to give the title compound (0.084 g, yield 82%).
  • 1H-NMR (CDCl3) δ: 1.07 (3H, t, J=7.4 Hz), 1.66 (3H, br s), 1.81-1.90 (2H, m), 3.45 (3H, br s), 4.02 (2H, t, J=6.5 Hz), 4.19 (3H, s), 4.50 (1H, dd, J=15.0, 5.8 Hz), 4.59 (1H, dd, J=15.0, 6.3 Hz), 6.97-7.02 (2H, m), 7.14 (1H, br s), 7.35 (1H, dd, J=8.3, 2.3 Hz), 7.51 (1H, d, J=8.3 Hz), 7.92 (1H, d, J=2.3 Hz), 8.52-8.56 (2H, m).
    • (2) (R)—N-{4-chloro-3-[4-hydroxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide (Example No. 1-137)
  • Figure US20200087266A1-20200319-C00195
  • Under an argon atmosphere, to a solution of (R)—N-{4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide (0.084 g, 0.16 mmol) obtained in the above-mentioned (1) in methanol (0.80 ml) was added 4M aqueous sodium hydroxide solution (0.30 ml) at room temperature, and the mixture was stirred at 65° C. for 1.5 hr. To the reaction mixture were added 2N hydrochloric acid (0.60 ml) and water, and the mixture was stirred at room temperature. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.072 g, yield 89%).
    • Production Example 21 Synthesis of N-{4-chloro-3-[4-(3,4-dimethylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-150)
  • Figure US20200087266A1-20200319-C00196
    • (1) 2-chloro-4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00197
  • Under an argon atmosphere, to a suspension of 3,4-dimethylbenzeneboronic acid (0.42 g, 2.8 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (1.0 g, 5.6 mmol) and tetrakis(triphenylphosphine)palladium(0) (0.32 g, 0.28 mmol) in toluene (8.4 ml) was added 2M aqueous sodium carbonate solution (4.2 ml), and the mixture was stirred at 100° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=20/1) to give the title compound (0.64 g, 92%).
  • 1H-NMR (CDCl3) δ: 2.35 (6H, s), 4.16 (3H, s), 7.26 (3H, d, J=7.8 Hz), 8.22 (1H, dd, J=7.8, 2.1 Hz), 8.25 (1H, d, J=2.1 Hz).
    • (2) {4-chloro-3-[4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol
  • Figure US20200087266A1-20200319-C00198
  • Under an argon atmosphere, to a solution of 2-chloro-4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazine (0.64 g, 2.6 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.57 g, 3.1 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.21 g, 0.26 mmol) in 1,4-dioxane (10 ml) was added 2M aqueous sodium carbonate solution (5.1 ml), and the mixture was stirred at 100° C. for 1 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=5/3) to give the title compound (0.54 g, yield 59%).
  • 1H-NMR (CDCl3) δ: 1.87 (1H, t, J=5.0 Hz), 2.35 (3H, s), 2.36 (3H, s), 4.20 (3H, s), 4.76 (2H, d, J=5.0 Hz), 7.27 (2H, d, J=8.2 Hz), 7.45 (1H, dd, J=8.4, 1.6 Hz), 7.53 (1H, d, J=8.4 Hz), 7.99 (1H, d, J=1.6 Hz), 8.33 (1H, d, J=8.2 Hz), 8.35 (1H, br s).
    • (3) 4-chloro-3-[4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride
  • Figure US20200087266A1-20200319-C00199
  • Under an argon atmosphere, to a solution of {4-chloro-3-[4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.54 g, 1.5 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (5.5 ml) were added triethylamine (0.28 ml, 2.0 mmol) and methanesulfonyl chloride (0.14 ml, 1.8 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (5.5 ml) were added cesium carbonate (1.5 g, 4.6 mmol) and di-tert-butyl iminodicarboxylate (0.40 g, 1.8 mmol) under ice-cooling, and the mixture was stirred at room temperature for 1 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=7/1). Under an argon atmosphere, to the purified product was added 4M hydrogen chloride/1,4-dioxane solution (6.5 ml) at room temperature, and the mixture was stirred for 0.5 hr. To the reaction mixture was added ethyl acetate, and the solid was collected by filtration, and dried under reduced pressure to give the title compound (0.56 g, yield 94%).
  • 1H-NMR (DMSO-D6) δ: 2.34 (3H, s), 2.35 (3H, s), 4.12-4.19 (5H, m), 7.38 (1H, d, J=7.9 Hz), 7.69-7.75 (2H, m), 8.12 (1H, d, J=1.9 Hz), 8.26 (1H, dd, J=7.9, 1.6 Hz), 8.29 (1H, br s), 8.44 (3H, br s).
    • (4) N-{4-chloro-3-[4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00200
  • Under an argon atmosphere, to a solution of 4-chloro-3-[4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.070 g, 0.18 mmol) obtained in the above-mentioned (3), HOBt.H2O (0.035 g, 0.23 mmol) and WSC.HCl (0.044 g, 0.23 mmol) in N,N-dimethylformamide (2.0 ml) were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.036 g, 0.23 mmol) and triethylamine (0.075 ml, 0.54 mmol) at room temperature, and the mixture was stirred for 4 hr. To the reaction mixture were added saturated aqueous sodium hydrogen carbonate and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=2/1) to give the title compound (0.075 g, yield 85%).
  • 1H-NMR (CDCl3) δ: 1.44 (6H, s), 2.36 (3H, s), 2.37 (3H, s), 4.20 (3H, s), 4.55 (2H, d, J=5.7 Hz), 6.22 (1H, br s), 7.27 (3H, d, J=7.8 Hz), 7.35 (1H, dd, J=8.2, 2.2 Hz), 7.52 (1H, d, J=8.2 Hz), 7.91 (1H, d, J=2.2 Hz), 8.32 (1H, dd, J=7.8, 1.7 Hz), 8.35 (1H, br s).
    • (5) N-{4-chloro-3-[4-(3,4-dimethylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-150)
  • Figure US20200087266A1-20200319-C00201
  • Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.075 g, 0.15 mmol) obtained in the above-mentioned (4) in methanol (1.8 ml) was added 4M aqueous sodium hydroxide solution (0.23 ml) at room temperature, and the mixture was stirred at 60° C. for 4 hr. To the reaction mixture were added 10% aqueous citric acid solution (1.0 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.063 g, yield 86%).
    • Production Example 22 Synthesis of N-{4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methyl-2-trifluoromethylpropionamide (Example No. 1-169)
  • Figure US20200087266A1-20200319-C00202
    • (1) 2-chloro-4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazine
  • Figure US20200087266A1-20200319-C00203
  • Under an argon atmosphere, to a suspension of 4-(cyclopropylmethoxy)benzeneboronic acid (2.5 g, 13 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (4.7 g, 26 mmol) and tetrakis(triphenylphosphine)palladium(0) (1.5 g, 1.3 mmol) in toluene (25 ml) was added 2M aqueous sodium carbonate solution (20 ml), and the mixture was stirred at 100° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=90/10−80/20) to give the title compound (3.0 g, 79%).
  • 1H-NMR (CDCl3) δ: 0.36-0.41 (2H, m), 0.65-0.71 (2H, m), 1.25-1.36 (1H, m), 3.90 (2H, d, J=7.0 Hz), 4.14 (3H, s), 6.96-7.00 (2H, m), 8.42-8.47 (2H, m).
    • (2) {4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol
  • Figure US20200087266A1-20200319-C00204
  • Under an argon atmosphere, to a solution of 2-chloro-4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazine (3.0 g, 10 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (2.3 g, 12 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.84 g, 1.0 mmol) in 1,4-dioxane (30 ml) was added 2M aqueous sodium carbonate solution (21 ml), and the mixture was stirred at 100° C. for 3 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=8/2−1/1) to give the title compound (2.9 g, yield 71%).
  • 1H-NMR (CDCl3) δ: 0.37-0.41 (2H, m), 0.65-0.71 (2H, m), 1.27-1.36 (1H, m), 1.76 (1H, t, J=6.0 Hz), 3.90 (2H, d, J=6.7 Hz), 4.19 (3H, s), 4.77 (2H, d, J=6.0 Hz), 6.98-7.02 (2H, m), 7.46 (1H, dd, J=8.1, 1.9 Hz), 7.53 (1H, d, J=8.1 Hz), 8.00 (1H, d, J=1.9 Hz), 8.53-8.57 (2H, m).
    • (3) 4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride
  • Figure US20200087266A1-20200319-C00205
  • Under an argon atmosphere, to a solution of {4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (2.9 g, 7.3 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (29 ml) were added triethylamine (1.3 ml, 9.5 mmol) and methanesulfonyl chloride (0.68 ml, 8.7 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (29 ml) were added cesium carbonate (7.1 g, 22 mmol) and di-tert-butyl iminodicarboxylate (1.9 g, 8.7 mmol) under ice-cooling, and the mixture was stirred at room temperature for 2 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=95/5−70/30). Under an argon atmosphere, to a solution (9.3 ml) of the purified product in 1,4-dioxane was added 4M hydrogen chloride/1,4-dioxane solution (37 ml) at room temperature, and the mixture was stirred for 3 hr. To the reaction mixture was added ethyl acetate, and the solid was collected by filtration, and dried under reduced pressure to give the title compound (3.1 g, yield 97%).
  • 1H-NMR (DMSO-D6) δ: 0.34-0.39 (2H, m), 0.57-0.63 (2H, m), 1.21-1.32 (1H, m), 3.95 (2H, d, J=7.0 Hz), 4.11-4.18 (5H, m), 7.11-7.15 (2H, m), 7.70-7.74 (2H, m), 8.13 (1H, br s), 8.42-8.53 (5H, m).
    • (4) N-{4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methyl-2-trifluoromethylpropionamide
  • Figure US20200087266A1-20200319-C00206
  • Under an argon atmosphere, to a solution of 4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.080 g, 0.18 mmol) obtained in the above-mentioned (3), HOBt.H2O (0.037 g, 0.24 mmol) and WSC.HCl (0.046 g, 0.24 mmol) in N,N-dimethylformamide (2.0 ml) were added 2,2-bis(trifluoromethyl)propionic acid (0.050 g, 0.24 mmol) and triethylamine (0.077 ml, 0.55 mmol) at room temperature, and the mixture was stirred for 1.5 hr. To the reaction mixture were added HOBt.H2O (0.037 g, 0.24 mmol), WSC.HCl (0.046 g, 0.24 mmol), 2,2-bis(trifluoromethyl)propionic acid (0.050 g, 0.24 mmol) and triethylamine (0.077 ml, 0.55 mmol), and the mixture was stirred for 2 hr. To the reaction mixture were added HOBt.H2O (0.037 g, 0.24 mmol), WSC.HCl (0.046 g, 0.24 mmol), 2,2-bis(trifluoromethyl)propionic acid (0.050 g, 0.24 mmol) and triethylamine (0.077 ml, 0.55 mmol), and the mixture was stirred for 1.5 hr. To the reaction mixture were added saturated aqueous sodium hydrogen carbonate and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=3/1) to give the title compound (0.045 g, yield 41%).
  • 1H-NMR (CDCl3) δ: 0.36-0.41 (2H, m), 0.65-0.71 (2H, m), 1.26-1.35 (2H, m), 1.70 (3H, s), 3.90 (2H, d, J=6.7 Hz), 4.19 (3H, s), 4.61 (2H, d, J=5.8 Hz), 6.49 (1H, br s), 6.98-7.02 (2H, m), 7.32 (1H, dd, J=8.5, 2.1 Hz), 7.53 (1H, d, J=8.5 Hz), 7.92 (1H, d, J=2.1 Hz), 8.52-8.56 (2H, m).
    • (5) N-{4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methyl-2-trifluoromethylpropionamide (Example No. 1-169)
  • Figure US20200087266A1-20200319-C00207
  • Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methyl-2-trifluoromethylpropionamide (0.045 g, 0.076 mmol) obtained in the above-mentioned (4) in methanol (0.70 ml) was added 4M aqueous sodium hydroxide solution (0.11 ml) at room temperature, and the mixture was stirred at 60° C. for 4 hr. To the reaction mixture were added 10% aqueous citric acid solution (0.50 ml) and water, and the mixture was stirred at room temperature. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.039 g, yield 89%).
    • Production Example 23 Synthesis of 1-trifluoromethylcyclopropanecarboxylic acid 4-chloro-3-[4-hydroxy-6-(4-isobutoxyphenyl)-1,3,5-triazin-2-yl]benzylamide (Example No. 1-178)
  • Figure US20200087266A1-20200319-C00208
    • (1) 1-trifluoromethylcyclopropanecarboxylic acid 4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamide
  • Figure US20200087266A1-20200319-C00209
  • Under an argon atmosphere, to a solution of 4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.10 g, 0.23 mmol) obtained in [Production Example 12] (4), HOBt.H2O (0.049 g, 0.32 mmol) and WSC.HCl (0.061 g, 0.32 mmol) in N,N-dimethylformamide (0.75 ml) were added 1-trifluoromethylcyclopropane-1-carboxylic acid (0.050 g, 0.32 mmol) and triethylamine (0.064 ml, 0.46 mmol) at room temperature, and the mixture was stirred for 1.5 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=2/1, then chloroform/ethyl acetate=9/1) to give the title compound (0.068 g, yield 55%).
  • 1H-NMR (DMSO-D6) δ: 1.01 (6H, d, J=6.9 Hz), 1.23-1.27 (2H, m), 1.30-1.36 (2H, m), 2.00-2.11 (1H, m), 3.87 (2H, d, J=6.4 Hz), 4.12 (3H, s), 4.37 (2H, d, J=5.9 Hz), 7.11-7.15 (2H, m), 7.43 (1H, dd, J=8.2, 2.1 Hz), 7.60 (1H, d, J=8.2 Hz), 7.85 (1H, d, J=2.1 Hz), 8.43-8.47 (2H, m), 8.50 (1H, t, J=5.9 Hz).
    • (2) 1-trifluoromethyl-cyclopropanecarboxylic acid 4-chloro-3-[4-hydroxy-6-(4-isobutoxyphenyl)-1,3,5-triazin-2-yl]benzylamide (Example No. 1-178)
  • Figure US20200087266A1-20200319-C00210
  • Under an argon atmosphere, to a solution of 1-trifluoromethylcyclopropanecarboxylic acid 4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamide (0.065 g, 0.12 mmol) obtained in the above-mentioned (1) in methanol (1.0 ml) was added 4M aqueous sodium hydroxide solution (0.12 ml) at room temperature, and the mixture was stirred at 60° C. for 3 hr. To the reaction mixture were added 2N hydrochloric acid (0.24 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.060 g, yield 94%).
    • Production Example 24 Synthesis of N-(4-chloro-3-{4-[4-((S)-1-cyclopropylethoxy)phenyl]-6-hydroxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-184)
  • Figure US20200087266A1-20200319-C00211
    • (1) N-(4-chloro-3-{4-[4-((S)-1-cyclopropylethoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00212
  • Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-hydroxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.080 g, 0.17 mmol) obtained in the above-mentioned [Production Example 14] (6), (1R)-1-cyclopropylethan-1-ol (0.029 g, 0.33 mmol) and triphenylphosphine (0.087 g, 0.33 mmol) in tetrahydrofuran (1.0 ml) was added bis(2-methoxyethyl)azodicarboxylate (0.078 g, 0.33 mmol) under ice-cooling, and the mixture was stirred at room temperature for 17 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=2/1) to give the title compound (0.079 g, yield 86%).
  • 1H-NMR (CDCl3) δ: 0.28-0.36 (1H, m), 0.38-0.45 (1H, m), 0.53-0.63 (2H, m), 1.12-1.21 (1H, m), 1.41 (3H, d, J=6.0 Hz), 1.44 (6H, s), 3.95-4.05 (1H, m), 4.18 (3H, s), 4.54 (2H, d, J=5.6 Hz), 6.20 (1H, br s), 6.95-7.00 (2H, m), 7.34 (1H, dd, J=8.3, 1.9 Hz), 7.51 (1H, d, J=8.3 Hz), 7.91 (1H, d, J=1.9 Hz), 8.50-8.55 (2H, m).
    • (2) N-(4-chloro-3-{4-[4-((S)-1-cyclopropylethoxy)phenyl]-6-hydroxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-184)
  • Figure US20200087266A1-20200319-C00213
  • Under an argon atmosphere, to a solution of N-(4-chloro-3-{4-[4-((S)-1-cyclopropylethoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (0.079 g, 0.14 mmol) obtained in the above-mentioned (1) in methanol (1.3 ml) was added 4M aqueous sodium hydroxide solution (0.22 ml) at room temperature, and the mixture was stirred at 65° C. for 4 hr. To the reaction mixture were added 10% aqueous citric acid solution (0.90 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.072 g, yield 93%).
    • Production Example 25 Synthesis of N-(4-chloro-3-{4-[4-((R)-1-cyclopropylethoxy)phenyl]-6-hydroxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-185)
  • Figure US20200087266A1-20200319-C00214
    • (1) N-(4-chloro-3-{4-[4-((R)-1-cyclopropylethoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide
  • Figure US20200087266A1-20200319-C00215
  • Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-hydroxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.080 g, 0.17 mmol) obtained in the above-mentioned [Production Example 14] (6), (1S)-1-cyclopropylethan-1-ol (0.029 g, 0.33 mmol) and triphenylphosphine (0.087 g, 0.33 mmol) in tetrahydrofuran (1.0 ml) was added bis(2-methoxyethyl) azodicarboxylate (0.078 g, 0.33 mmol) under ice-cooling, and the mixture was stirred at room temperature for 17 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=2/1) to give the title compound (0.038 g, yield 41%).
  • 1H-NMR (CDCl3) δ: 0.28-0.36 (1H, m), 0.38-0.45 (1H, m), 0.53-0.63 (2H, m), 1.12-1.21 (1H, m), 1.41 (3H, d, J=6.0 Hz), 1.44 (6H, s), 3.95-4.05 (1H, m), 4.18 (3H, s), 4.54 (2H, d, J=5.6 Hz), 6.20 (1H, br s), 6.95-7.00 (2H, m), 7.34 (1H, dd, J=8.3, 1.9 Hz), 7.51 (1H, d, J=8.3 Hz), 7.91 (1H, d, J=1.9 Hz), 8.50-8.55 (2H, m).
    • (2) N-(4-chloro-3-{4-[4-((R)-1-cyclopropylethoxy)phenyl]-6-hydroxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-185)
  • Figure US20200087266A1-20200319-C00216
  • Under an argon atmosphere, to a solution of N-(4-chloro-3-{4-[4-((R)-1-cyclopropylethoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (0.038 g, 0.069 mmol) obtained in the above-mentioned (1) in methanol (0.62 ml) was added 4M aqueous sodium hydroxide solution (0.10 ml) at room temperature, and the mixture was stirred at 65° C. for 4 hr. To the reaction mixture were added 10% aqueous citric acid solution (0.42 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.034 g, yield 91%).
  • The compounds of Example 1-1 to Example 1-267, Example 2-1 to Example 2-130, and Example 3-1 to Example 3-23 were obtained according to the above-mentioned Production Methods. The structural formulas and property data of the Example compounds are shown in Table 1-1 to Table 1-34, Table 2-1 to Table 2-15, and Table 3-1 to Table 3-3. In the Tables, the notes show the following.
  • Figure US20200087266A1-20200319-C00217
    Figure US20200087266A1-20200319-C00218
    • Note 1 (Example No. 1-188, 1-189)
  • Using 2,4-dichloro-6-methoxy-1,3,5-triazine, 4-fluoro-3-methylphenylboronic acid instead of 4-(2,2-dimethylpropoxy)phenylboronic acid, and 5-acetyl-2-chlorophenylboronic acid instead of 2-chloro-5-hydroxymethylphenylboronic acid, and by a method similar to that in Production Example 1 (1) and (2), compound A was obtained.
  • Racemic compound B was obtained by reducing the carbonyl group of compound A with sodium borohydride.
  • Racemic compound D was obtained by treating compound B in the same manner as in Production Example 1 (3) and (4).
  • Compound F as a diastereomer mixture was obtained by reacting racemic compound D with pure enantiomer compound E.
  • Compound F1 which is a less polar diastereomer (Merck TLC Silica gel 60G F254 25 Glassplates, eluent: n-hexane/ethyl acetate=2/1) and compound F2 which is a more polar diastereomer were obtained by purifying compound F by silica gel column chromatography. While compound F1 and compound F2 are single stereoisomers, the absolute configuration of the asymmetric carbon at the benzyl position is undetermined.
  • The compound of Example No. 1-188 was obtained by hydrolyzing compound F1 in the same manner as in Production Example 1 (6). Similarly, the compound of Example No. 1-189 was obtained from compound F2. While the compound of Example No. 1-188 and the compound of Example No. 1-189 are single stereoisomers, the absolute configuration of the asymmetric carbon at the benzyl position is undetermined.
  • Figure US20200087266A1-20200319-C00219
    Figure US20200087266A1-20200319-C00220
    • Note 2 (Example No. 1-200, 1-201)
  • Using 2,4-dichloro-6-methoxy-1,3,5-triazine, 4-(2,2-dimethylpropoxy)phenylboronic acid, and 5-acetyl-2-chlorophenylboronic acid instead of 2-chloro-5-hydroxymethylphenylboronic acid, and by a method similar to that in Production Example 1 (1) and (2), compound J was obtained.
  • Racemic compound K was obtained by reducing the carbonyl group of compound J with sodium borohydride.
  • Racemic compound M was obtained by treating compound K in the same manner as in Production Example 1 (3) and (4).
  • Compound N as a diastereomer mixture was obtained by reacting racemic compound M with pure enantiomer compound E.
  • Compound N was purified by silica gel column chromatography in the same manner as in note 1, by a method similar to that in Production Example 1 (6), the compound of Example No. 1-200 was obtained from compound N1 which is a less polar diastereomer (Merck TLC Silica gel 60G F254 25 Glassplates, eluent: n-hexane/ethyl acetate=2/1), and the compound of Example No. 1-201 was obtained from compound N2 which is a more polar diastereomer. While the compounds of Example Nos. 1-200 and 1-201 are single stereoisomers, the absolute configuration of the asymmetric carbon at the benzyl position is undetermined.
    • Note 3 (Example Nos. 1-256, 1-257)
  • While they are single stereoisomers, the relative configuration thereof is undetermined.
    • Note 4 (Example No. 1-266)
  • While it is a single stereoisomer, the relative configuration of the tert-butyl group is undetermined.
    • Note 5 (Example No. 1-267)
  • While it is a single stereoisomer, the relative configuration of the methoxy group is undetermined.
  • TABLE 1-1
    Example Structure NMR MS(M + H) MS(M − H) Note
    1-1
    Figure US20200087266A1-20200319-C00221
         		1H-NMR (DMSO-D6) δ: 2.50 (3H, s), 7.38 (1H, td, J = 8.3, 2.5 Hz), 7.46 (1H, dd, J = 8.4, 5.3 Hz), 7.56-7.65 (1H, br m), 7.95 (2H, d, J = 8.4 Hz), 8.55 (2H, d, J = 8.4 Hz), 13.33 (1H, br s). 350 348
    1-2
    Figure US20200087266A1-20200319-C00222
         		1H-NMR (DMSO-D6) δ: 2.35 (3H, s), 2.50 (3H, s), 7.30 (1H, d, J = 7.7 Hz), 7.34 (1H, d, J = 7.7 Hz), 7.51 (1H, br s), 7.94 (2H, d, J = 8.1 Hz), 8.55 (2H, d, J = 8.1 Hz), 13.17 (1H, br s). 346 344
    1-3
    Figure US20200087266A1-20200319-C00223
         		1H-NMR (DMSO-D6) δ: 2.50 (3H, s), 7.45 (1H, d, J = 8.4 Hz), 7.59 (1H, dd, J = 8.4, 2.3 Hz), 7.81 (1H, br s), 7.94 (2H, d, J = 8.1 Hz), 8.54 (2H, d, J = 8.1 Hz), 13.33 (1H, s). 366 364
    1-4
    Figure US20200087266A1-20200319-C00224
         		1H-NMR (DMSO-D6) δ: 2.38 (3H, s), 7.46 (1H, d, J = 8.1 Hz), 7.55 (1H, d, J = 8.1 Hz), 7.64 (1H, br s), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.49 (1H, br s). 366 364
    1-5
    Figure US20200087266A1-20200319-C00225
         		1H-NMR (DMSO-D6) δ: 7.69 (1H, dd, J = 8.8, 2.2 Hz), 7.83 (1H, d, J = 8.8 Hz), 7.91 (1H, d, J = 2.2 Hz), 7.95 (2H, d, J = 8.2 Hz), 8.53 (2H, d, J = 8.2 Hz), 13.67 (1H, br s). 436 434
    1-6
    Figure US20200087266A1-20200319-C00226
         		1H-NMR (DMSO-D6) δ: 3.83 (3H, s), 7.21 (1H, dd, J = 8.8, 3.1 Hz), 7.41 (1H, d, J = 3.1 Hz), 7.57 (1H, d, J = 8.8 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.52 (1H, br s). 382 380
    1-7
    Figure US20200087266A1-20200319-C00227
         		1H-NMR (DMSO-D6) δ: 7.01 (1H, dd, J = 8.6, 2.9 Hz), 7.17 (1H, s), 7.44 (1H, d, J = 8.6 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 10.17 (1H, s), 13.46 (1H, br s). 368 366
    1-8
    Figure US20200087266A1-20200319-C00228
         		1H-NMR (DMSO-D6) δ: 7.81 (1H, d, J = 8.4 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.12 (1H, dd, J = 8.5, 2.0 Hz), 8.36 (1H, d, J = 1.9 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.53 (2H, br s). 396 394
  • TABLE 1-2
    1-9
    Figure US20200087266A1-20200319-C00229
         		1H-NMR (DMSO-D6) δ: 2.63 (3H, s), 7.66 (1H, d, J = 8.2 Hz), 7.88 (1H, dd, J = 8.3, 1.7 Hz), 7.95 (2H, d, J = 8.4 Hz), 8.11 (1 H, br s), 8.54 (2H, d, J = 8.4 Hz), 13.41 (1H, br s). 400 398
    1-10
    Figure US20200087266A1-20200319-C00230
         		1H-NMR (DMSO-D6) δ: 2.61 (3H, s), 7.54 (1H, d, J = 7.9 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.04 (1H, dd, J = 7.9, 1.8 Hz), 8.30 (1H, br s), 8.55 (2H, d, J = 8.4 Hz), 13.23 (2H, br s). 376 374
    1-11
    Figure US20200087266A1-20200319-C00231
         		1H-NMR (DMSO-D6) δ: 2.59 (3H, s), 7.46 (1H, br s), 7.50 (1H, d, J = 8.2 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.00 (1H, dd, J = 7.9, 1.8 Hz), 8.01 (1H, br s), 8.24 (1H, br s), 8.56 (2H, d, J = 8.4 Hz), 13.28 (1H, s). 375 373
    1-12
    Figure US20200087266A1-20200319-C00232
         		1H-NMR (DMSO-D6) δ: 4.58 (2H, d, J = 5.5 Hz), 5.45 (1H, t, J = 5.6 Hz), 7.56 (1H, dd, J = 8.2, 2.0 Hz), 7.62 (1H, d, J = 8.2 Hz), 7.74 (1H, br s), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.53 (1H, br s). 382 380
    1-13
    Figure US20200087266A1-20200319-C00233
         		1H-NMR (DMSO-D6) δ: 2.65 (3H, s), 7.84 (1H, d, J = 8.4 Hz), 7.95 (2H, d, J = 8.4 Hz), 8.16 (1H, dd, J = 8.4, 2.2 Hz), 8.38 (1H, d, J 2.2 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.65 (1H, s). 394 392
    1-14
    Figure US20200087266A1-20200319-C00234
         		1H-NMR (DMSO-D6) δ: 1.22 (3H, t, J = 7.7 Hz), 2.50 (3H, s), 2.66 (2H, q, J = 7.6 Hz), 7.32 (1H, d, J = 7.9 Hz), 7.37 (1H, dd, J = 7.8, 1.7 Hz), 7.55 (1H, br s), 7.94 (2H, d, J = 8.4 Hz), 8.55 (2H, d, J = 8.4 Hz), 13.19 (1H, br s). 360 358
    1-15
    Figure US20200087266A1-20200319-C00235
         		1H-NMR (DMSO-D6) δ: 1.39 (9H, s), 4.20 (2H, d, J = 6.0 Hz), 7.47-7.53 (2H, m), 7.62 (1H, d, J = 8.4 Hz), 7.66 (1H, br s), 7.93 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.54 (1H, s). 481 479
    1-16
    Figure US20200087266A1-20200319-C00236
         		1H-NMR (DMSO-D6) δ: 1.89 (3H, s), 4.32 (2H, d, J = 6.0 Hz), 7.49 (1H, dd, J = 8.3, 2.3 Hz), 7.62 (1H, d, J = 8.2 Hz), 7.67 (1H, d, J = 2.2 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.46 (1H, t, J = 6.0 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s). 423 421
  • TABLE 1-3
    1-17
    Figure US20200087266A1-20200319-C00237
         		1H-NMR (DMSO-D6) δ: 4.13 (2H, br s), 7.72- 7.77 (2H, m), 7.93-7.97 (3H, m), 8.39 (3H, br s), 8.54 (2H, d, J = 8.4 Hz), 13.67 (1H, s). 381 379
    1-18
    Figure US20200087266A1-20200319-C00238
         		1H-NMR (DMSO-D6) δ: 1.36 (3H, d, J = 6.4 Hz), 4.78-4.84 (1H, m), 5.42 (1H, d, J = 4.2 Hz), 7.57-7.62 (2H, m), 7.77 (1H, br s), 7.94 (2H, d, J = 8.2 Hz), 8.54 (2H, d, J = 8.2 Hz), 13.52 (1H, br s). 396 394
    1-19
    Figure US20200087266A1-20200319-C00239
         		1H-NMR (DMSO-D6) δ: 3.34 (3H, s), 4.50 (2H, s), 7.57 (1H, dd, J = 8.3, 2.1 Hz), 7.65 (1H, d, J = 8.2 Hz), 7.76 (1H, d, J = 2.0 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.54 (1H, br s). 396 394
    1-20
    Figure US20200087266A1-20200319-C00240
         		1H-NMR (DMSO-D6) δ: 2.93 (3H, s), 4.25 (2H, d, J = 6.4 Hz), 7.60 (1H, dd, J = 8.4, 2.0 Hz), 7.66 (1H, d, J = 8.4 Hz), 7.70 (1H, t, J = 6.4 Hz), 7.78 (1H, d, J = 2.0 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.58 (1H, br s). 459 457
    1-21
    Figure US20200087266A1-20200319-C00241
         		1H-NMR (DMSO-D6) δ: 2.07 (1.0H, s), 2.08 (2.0H, s), 2.82 (1 .OH, s), 2.96 (2.0H, s), 4.56 (1.3H, s), 4.64 (0.7H, s), 7.47 (1.0H, d, J = 8.2 Hz), 7.61-7.68 (2.0H, m), 7.94 (2.0H, d, J = 8.4 Hz), 8.53 (2.0H, d, J = 8.4 Hz), 13.56 (1.0H, s). 437 435
    1-22
    Figure US20200087266A1-20200319-C00242
         		1H-NMR (DMSO-D6) δ: 3.56 (3H, s), 4.26 (2H, d, J = 6.2 Hz), 7.50 (1H, dd, J = 8.5, 2.1 Hz), 7.63 (1H, d, J = 8.4 Hz), 7.68 (1H, d, J = 2.0 Hz), 7.80 (1H, t, J = 6.2 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.54 (1H, s). 439 437
    1-23
    Figure US20200087266A1-20200319-C00243
         		1H-NMR (DMSO-D6) δ: 3.71 (2H, s), 7.53 (1H, dd, J = 8.3, 1.9 Hz), 7.62 (1H, d, J = 8.2 Hz), 7.71 (1H, d, J = 1.8 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 12.53 (1H, br s), 13.54 (1H, br s). 410 408
    1-24
    Figure US20200087266A1-20200319-C00244
         		1H-NMR (DMSO-D6) δ: 1.13 (9H, s), 4.32 (2H, d, J = 6.0 Hz), 7.45 (1H, dd, J = 8.3, 2.1 Hz), 7.61 (1H, d, J = 8.2 Hz), 7.65 (1H, d, J = 2.0 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.18 (1H, t, J = 6.1 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s). 465 463
  • TABLE 1-4
    1-25
    Figure US20200087266A1-20200319-C00245
         		1H-NMR (DMSO-D6) δ: 2.09 (3H, s), 5.16 (2H, s), 7.63 (1H, dd, J = 8.3, 2.1 Hz), 7.68 (1H, d, J = 8.2 Hz), 7.81 (1H, d, J = 2.0 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.57 (1H, br s). 424 422
    1-26
    Figure US20200087266A1-20200319-C00246
         		1H-NMR (DMSO-D6) δ: 2.59 (3H, d, J = 4.6 Hz), 3.50 (2H, s), 7.50 (1H, dd, J = 8.3, 2.1 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.67 (1H, d, J = 2.0 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.01-8.06 (1H, br m), 8.54 (2H, d, J = 8.4 Hz), 13.54 (1H, s). 423 421
    1-27
    Figure US20200087266A1-20200319-C00247
         		1H-NMR (DMSO-D6) δ: 1.02 (3H, t, J = 7.6 Hz), 2.16 (2H, q, J = 7.6 Hz), 4.32 (2H, d, J = 6.0 Hz), 7.49 (1H, dd, J = 8.4, 2.2 Hz), 7.62 (1H, d, J = 8.4 Hz), 7.67 (1H, d, J = 2.2 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.39 (1H, t, J = 6.0 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s). 437 435
    1-28
    Figure US20200087266A1-20200319-C00248
         		1H-NMR (DMSO-D6) δ: 1.04 (6H, d, J = 7.1 Hz), 2.38-2.48 (1H, m), 4.32 (2H, d, J = 6.2 Hz), 7.47 (1H, dd, J = 8.4, 2.2 Hz), 7.62 (1H, d, J = 8.4 Hz), 7.66 (1H, d, J = 2.2 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.37 (1H, t, J = 6.2 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s). 451 449
    1-29
    Figure US20200087266A1-20200319-C00249
         		1H-NMR (DMSO-D6) δ: 1.11-1.40 (5H, m), 1.57-1.76 (5H, m), 2.13-2.20 (1H, m), 4.31 (2H, d, J = 6.2 Hz), 7.46 (1H, dd, J = 8.3, 2.1 Hz), 7.61 (1H, d, J = 8.2 Hz), 7.65 (1H, d, J = 2.0 Hz), 7.93 (2H, d, J = 8.4 Hz), 8.34 (1H, t, J = 6.1 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s). 491 489
    1-30
    Figure US20200087266A1-20200319-C00250
         		1H-NMR (DMSO-D6) δ: 1.44-1.82 (8H, m), 2.58-2.66 (1H, m), 4.32 (2H, d, J = 6.2 Hz), 7.47 (1H, dd, J = 8.3, 2.1 Hz), 7.62 (1H, d, J = 8.2 Hz), 7.66 (1H, d, J = 2.0 Hz), 7.93 (2H, d, J = 8.4 Hz), 8.39 (1H, t, J = 6.2 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s). 477 475
    1-31
    Figure US20200087266A1-20200319-C00251
         		1H-NMR (DMSO-D6) δ: 1.20 (3H, t, J = 7.3 Hz), 3.02 (2H, q, J = 7.4 Hz), 4.23 (2H, d, J = 6.5 Hz), 7.60 (1H, dd, J = 8.4, 2.1 Hz), 7.66 (1H, d, J = 8.4 Hz), 7.72 (1H, t, J = 6.4 Hz), 7.77 (1H, d, J = 2.1 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.57 (1H, br s). 473 471
    1-32
    Figure US20200087266A1-20200319-C00252
         		1H-NMR (DMSO-D6) δ:1.31 (9H, s), 4.32 (2H, d, J = 6.4 Hz), 7.60-7.66 (3H, m), 7.76 (1H, br s), 7.94 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.58 (1H, br s). 501 499
  • TABLE 1-5
    1-33
    Figure US20200087266A1-20200319-C00253
         		1H-NMR (DMSO-D6) δ: 1.24 (6H, d, J = 6.8 Hz), 3.14-3.20 (1H, m), 4.25 (2H, d, J = 6.4 Hz), 7.60 (1H, dd, J = 8.5, 1.9 Hz), 7.66 (1H, d, J = 8.4 Hz), 7.70 (1H, t, J = 6.5 Hz), 7.77 (1H, d, J = 1.8 Hz), 7.94 (2H, d, J = 8.2 Hz), 8.53 (2H, d, J = 8.2 Hz), 13.58 (1H, br s). 487 485
    1-34
    Figure US20200087266A1-20200319-C00254
         		1H-NMR (DMSO-D6) δ: 2.81 (6H, s), 4.28 (2H, d, J = 5.8 Hz), 6.98 (1H, t, J = 5.9 Hz), 7.50 (1H, dd, J = 8.3, 2.0 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.68 (1H, d, J = 1.9 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.54 (1H, br s). 452 450
    1-35
    Figure US20200087266A1-20200319-C00255
         		1H-NMR (DMSO-D6) δ: 2.56 (3H, d, J = 4.6 Hz), 4.26 (2H, d, J = 6.2 Hz), 5.90 (1H, q, J = 4.6 Hz), 6.52 (1H, t, J = 6.2 Hz), 7.49 (1H, dd, J = 8.3, 2.1 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.66 (1H, d, J = 2.0 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s). 438 436
    1-36
    Figure US20200087266A1-20200319-C00256
         		1H-NMR (DMSO-D6) δ: 0.85 (3H, t, J = 7.4 Hz), 1.54 (2H, sextet, J = 7.4 Hz), 2.13 (2H, t, J = 7.4 Hz), 4.33 (2H, d, J = 6.2 Hz), 7.48 (1H, dd, J = 8.2, 2.0 Hz), 7.62 (1H, d, J = 8.2 Hz), 7.66 (1H, d, J = 2.0 Hz), 7.94 (2H, d, J = 8.2 Hz), 8.41 (1H, t, J = 6.1 Hz), 8.53 (2H, d, J = 8.2 Hz), 13.55 (1H, br s). 451 449
    1-37
    Figure US20200087266A1-20200319-C00257
         		1H-NMR (DMSO-D6) δ: 0.87 (6H, d, J = 6.4 Hz), 1.96-2.06 (3H, m), 4.33 (2H, d, J = 6.0 Hz), 7.48 (1H, dd, J = 8.2, 2.0 Hz), 7.61 (1H, d, J = 8.2 Hz), 7.66 (1H, d, J = 2.0 Hz), 7.93 (2H, d, J = 8.4 Hz), 8.42 (1H, t, J = 6.1 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s). 465 463
    1-38
    Figure US20200087266A1-20200319-C00258
         		1H-NMR (DMSO-D6) δ: 1.16 (3H, t, J = 7.1 Hz), 4.01 (2H, q, J = 7.1 Hz), 4.25 (2H, d, J = 6.2 Hz), 7.50 (1H, dd, J = 8.4, 2.0 Hz), 7.63 (1H, d, J = 8.4 Hz), 7.68 (1H, d, J = 2.0 Hz), 7.76 (1H, t, J = 6.3 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s). 453 451
    1-39
    Figure US20200087266A1-20200319-C00259
         		1H-NMR (DMSO-D6) δ: 1.17 (6H, d, J = 6.2 Hz), 4.24 (2H, d, J = 6.2 Hz), 4.73-4.80 (1H, m), 7.49 (1H, dd, J = 8.4, 2.0 Hz), 7.63 (1H, d, J = 8.4 Hz), 7.66-7.71 (2H, m), 7.93 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s). 467 465
    1-40
    Figure US20200087266A1-20200319-C00260
         		1H-NMR (DMSO-D6) δ: 2.85 (3H, s), 3.05 (3H, s), 3.80 (2H, s), 7.47 (1H, dd, J = 8.4, 2.2 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.65 (1H, d, J = 2.2 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.54 (1H, br s). 437 435
  • TABLE 1-6
    1-41
    Figure US20200087266A1-20200319-C00261
         		1H-NMR (DMSO-D6) δ: 1.25 (9H, s), 3.47 (2H, s), 7.49 (1H, dd, J = 8.5, 2.1 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.66 (1H, d, J = 2.0 Hz), 7.77 (1H, s), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.54 (1H, s). 465 463
    1-42
    Figure US20200087266A1-20200319-C00262
         		1H-NMR (DMSO-D6) δ: 1.51-1.71 (4H, m), 1.83-1.92 (4H, m), 3.47-3.54 (1H, m), 4.26 (2H, d, J = 6.4 Hz), 7.59 (1H, d, J = 8.3 Hz), 7.66 (1H, d, J = 8.3 Hz), 7.72 (1H, t, J = 6.3 Hz), 7.77 (1H, s), 7.94 (2H, d, J = 8.3 Hz), 8.53 (2H, d, J = 8.3 Hz), 13.58 (1H, br s). 513 511
    1-43
    Figure US20200087266A1-20200319-C00263
         		1H-NMR (DMSO-D6) δ: 1.13 (9H, s), 4.31 (2H, d, J = 6.0 Hz), 7.44 (1H, dd, J = 8.2, 2.0 Hz), 7.56-7.58 (3H, m), 7.64-7.69 (2H, m), 8.18 (1H, t, J = 6.0 Hz), 8.34 (2H, d, J = 7.1 Hz), 13.34 (1H, br s). 397 395
    1-44
    Figure US20200087266A1-20200319-C00264
         		1H-NMR (DMSO-D6) δ: 0.91 (3H, t, J = 7.4 Hz), 1.13 (9H, s), 1.62-1.65 (2H, m), 2.65 (2H, t, J = 7.5 Hz), 4.31 (2H, d, J = 6.0 Hz), 7.38 (2H, d, J = 8.2 Hz), 7.43 (1H, dd, J = 8.4, 2.0 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.64 (1H, br s), 8.17 (1H, t, J = 6.1 Hz), 8.26 (2H, d, J = 8.2 Hz), 13.25 (1H, br s). 439 437
    1-45
    Figure US20200087266A1-20200319-C00265
         		1H-NMR (DMSO-D6) δ: 2.51 (3H, s), 4.55 (2H, d, J = 5.5 Hz), 5.29 (1H, t, J = 5.6 Hz), 7.36 (1H, d, J = 7.9 Hz), 7.46 (1H, dd, J = 7.8, 1.7 Hz), 7.65 (1H, br s), 7.94 (2H, d, J = 8.4 Hz), 8.55 (2H, d, J = 8.4 Hz), 13.22 (1H, br s). 362 360
    1-46
    Figure US20200087266A1-20200319-C00266
         		1H-NMR (DMSO-D6) δ: 1.13 (9H, s), 1.24 (6H, d, J = 6.8 Hz), 2.96-3.03 (1H, m), 4.31 (2H, d, J = 6.0 Hz), 7.41-7.46 (3H, m), 7.58 (1H, d, J = 8.4 Hz), 7.64 (1H, br s), 8.17 (1H, t, J = 6.0 Hz), 8.27 (2H, d, J = 8.2 Hz), 13.26 (1H, br s). 439 437
    1-47
    Figure US20200087266A1-20200319-C00267
         		1H-NMR (DMSO-D6) δ: 1.88 (3H, s), 2.50 (3H, s), 4.29 (2H, d, J = 6.0 Hz), 7.35 (1H, d, J = 7.9 Hz), 7.39 (1H, dd, J = 7.9, 1.8 Hz), 7.59 (1H, br s), 7.94 (2H, d, J = 8.2 Hz), 8.37 (1H, t, J = 6.0 Hz), 8.54 (2H, d, J = 8.2 Hz), 13.26 (1H, br s). 403 401
    1-48
    Figure US20200087266A1-20200319-C00268
         		1H-NMR (DMSO-D6) δ: 1.13 (9H, s), 2.50 (3H, s), 4.30 (2H, d, J = 6.0 Hz), 7.34-7.35 (2H, br m), 7.57 (1H, br s), 7.93 (2H, d, J = 8.4 Hz), 8.09 (1H, t, J = 6.1 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.24 (1H, br s). 445 443
  • TABLE 1-7
    1-49
    Figure US20200087266A1-20200319-C00269
         		1H-NMR (DMSO-D6) δ: 1.13 (9H, s), 1.25 (6H, d, J = 7.0 Hz), 2.96-3.03 (1H, m), 4.32 (2H, d, J = 5.8 Hz), 7.44 (1H, dd, J = 8.1, 1.2 Hz), 7.48 (1H, t, J = 7.7 Hz), 7.56 (1H, d, J = 7.7 Hz), 7.59 (1H, d, J = 8.1 Hz), 7.65 (1H, br s), 8.16-8.20 (3H, m), 13.30 (1H, br s). 439 437
    1-50
    Figure US20200087266A1-20200319-C00270
         		1H-NMR (DMSO-D6) δ: 0.91 (3H, t, J = 7.3 Hz), 1.13 (9H, s), 1.61-1.65 (2H, m), 2.65 (2H, t, J = 7.5 Hz), 4.31 (2H, d, J = 6.2 Hz), 7.43-7.50 (3H, m), 7.59 (1H, d, J = 8.2 Hz), 7.64 (1H, br s), 8.16-8.18 (3H, m), 13.30 (1H, s). 439 437
    1-51
    Figure US20200087266A1-20200319-C00271
         		1H-NMR (DMSO-D6) δ: 0.88 (6H, d, J = 6.6 Hz), 1.13 (9H, s), 1.88-1.92 (1H, m), 2.55 (2H, d, J = 7.1 Hz), 4.31 (2H, d, J = 6.2 Hz), 7.35 (2H, d, J = 8.2 Hz), 7.43 (1H, dd, J = 8.4, 2.0 Hz), 7.58 (1H, d, J = 8.2 Hz), 7.64 (1H, br s), 8.17 (1H, t, J = 6.1 Hz), 8.26 (2H, d, J = 8.4 Hz), 13.25 (1H, br s). 439 437
    1-52
    Figure US20200087266A1-20200319-C00272
         		1H-NMR (DMSO-D6) δ: 1.06 (3H, s), 1.14- 1.49 (8H, m), 1.91-1.98 (2H, m), 4.32 (2H, d, J = 5.8 Hz), 7.43 (1H, d, J = 8.1 Hz), 7.52- 7.59 (3H, m), 7.62-7.67 (2H, m), 8.16 (1H, t, J J = 5.9 Hz), 8.32 (2H, d, J = 8.1 Hz), 13.31 (1H, br s). 437 435
    1-53
    Figure US20200087266A1-20200319-C00273
         		1H-NMR (DMSO-D6) δ: 1.26 (6H, s), 3.15 (3H, s), 4.32 (2H, d, J = 6.3 Hz), 7.45 (1H, d, J = 7.2 Hz), 7.53-7.59 (3H, m), 7.63-7.68 (2H, m), 8.32 (2H, d, J = 7.9 Hz), 8.46 (1H, t, J = 6.3 Hz), 13.31 (1H, br s). 413 411
    1-54
    Figure US20200087266A1-20200319-C00274
         		1H-NMR (DMSO-D6) δ: 1.12 (9H, s), 4.31 (2H, d, J = 6.0 Hz), 7.45 (1H, d, J = 8.4 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.63 (1H, s), 7.81 (1H, t, J = 7.9 Hz), 8.03 (1H, d, J = 7.4 Hz), 8.16 (1H, t, J = 5.9 Hz), 8.57-8.63 (2H, m), 13.51 (1H, br s). 465 463
    1-55
    Figure US20200087266A1-20200319-C00275
         		1H-NMR (DMSO-D6) δ:1.13 (9H, s), 1.22 (3H, t, J = 7.6 Hz), 2.70 (2H, q, J = 7.6 Hz), 4.31 (2H, d, J = 6.0 Hz), 7.42-7.53 (3H, m), 7.59 (1H, d, J = 8.2 Hz), 7.64 (1H, s), 8.15- 8.19 (3H, m), 13.31 (1H, br s). 425 423
    1-56
    Figure US20200087266A1-20200319-C00276
         		1H-NMR (DMSO-D6) δ: 1.28 (6H, s), 3.17 (3H, s), 4.33 (2H, d, J = 6.2 Hz), 7.48 (1H, dd, J = 8.4, 2.1 Hz), 7.61 (1H, d, J = 8.3 Hz), 7.67 (1H, s), 7.94 (2H, d, J = 8.3 Hz), 8.48 (1H, t, J = 6.3 Hz), 8.52 (2H, d, J = 8.3 Hz), 13.55 (1H, br s). 481 479
  • TABLE 1-8
    1-57
    Figure US20200087266A1-20200319-C00277
         		1H-NMR (DMSO-D6) δ: 1.13 (9H, s), 1.34 (9H, s), 4.31 (2H, d, J = 6.0 Hz), 7.43 (1H, dd, J = 8.2, 2.0 Hz), 7.49 (1H, t, J = 7.8 Hz), 7.59 (1H, d, J = 8.2 Hz), 7.66 (1H, s), 7.72 (1H, d, J = 8.2 Hz), 8.15-8.18 (2H, m), 8.38 (1H, s), 13.33 (1H, br s). 453 451
    1-58
    Figure US20200087266A1-20200319-C00278
         		1H-NMR (DMSO-D6) δ: 0.72 (3H, t, J = 7.4 Hz), 1.08 (6H, s), 1.49 (2H, q, J = 7.4 Hz), 4.32 (2H, d, J = 6.0 Hz), 7.47 (1H, dd, J = 8.2, 2.0 Hz), 7.61 (1H, d, J = 8.2 Hz), 7.65 (1H, s), 7.82 (1H, t, J = 7.8 Hz), 8.04 (1H, d, J = 7.9 Hz), 8.15 (1H, t, J = 6.1 Hz), 8.59-8.63 (2H, m), 13.54 (1H, s). 479 477
    1-59
    Figure US20200087266A1-20200319-C00279
         		1H-NMR (DMSO-D6) δ: 1.37 (6H, s), 4.36 (2H, d, J = 5.6 Hz), 7.45 (1H, d, J = 8.4 Hz), 7.62 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 7.81 (1H, t, J = 7.7 Hz), 8.03 (1H, d, J = 7.9 Hz), 8.58-8.66 (3H, m), 13.53 (1H, br s). 519 517
    1-60
    Figure US20200087266A1-20200319-C00280
         		1H-NMR (DMSO-D6) δ: 1.13 (9H, s), 4.31 (2H, d, J = 6.0 Hz), 7.45 (1H, d, J = 8.4 Hz), 7.55 (2H, d, J = 8.7 Hz), 7.61 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.18 (1H, t, J = 6.0 Hz), 8.46 (2H, d, J = 8.7 Hz), 13.45 (1H, br s). 481 479
    1-61
    Figure US20200087266A1-20200319-C00281
         		1H-NMR (DMSO-D6) δ: 1.13 (9H, s), 4.31 (2H, d, J = 6.0 Hz), 7.45 (1H, dd, J = 8.4, 2.2 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.63-7.73 (3H, m), 8.18 (1H, t, J = 6.0 Hz), 8.21 (1H, s), 8.37 (1H, dt, J = 7.4, 1.5 Hz), 13.50 (1H, br s). 481 479
    1-62
    Figure US20200087266A1-20200319-C00282
         		1H-NMR (DMSO-D6) δ: 1.12 (9H, s), 1.34 (3H, t, J = 7.0 Hz), 4.09 (2H, q, J = 7.0 Hz), 4.30 (2H, d, J = 6.0 Hz), 7.20 (1H, dd, J = 8.1, 2.3 Hz), 7.41-7.46 (2H, m), 7.57 (1H, d, J = 8.4 Hz), 7.63 (1H, s), 7.84 (1H, s), 7.91 (1H, d, J = 7.7 Hz), 8.16 (1H, t, J = 6.0 Hz), 13.30 (1H, br s). 441 439
    1-63
    Figure US20200087266A1-20200319-C00283
         		1H-NMR (DMSO-D6) δ: 1.13 (9H, s), 2.41 (3H, s), 4.31 (2H, d, J = 6.0 Hz), 7.37 (2H, d, J = 8.1 Hz), 7.43 (1H, d, J = 8.1 Hz), 7.59 (1H, d, J = 8.1 Hz), 7.64 (1H, s), 8.17 (1H, t, J = 6.0 Hz), 8.24 (2H, d, J = 8.1 Hz), 13.24 (1H, br s). 411 409
    1-64
    Figure US20200087266A1-20200319-C00284
         		1H-NMR (DMSO-D6) δ: 1.14 (9H, s), 2.40 (3H, s), 4.32 (2H, d, J = 6.0 Hz), 7.41-7.51 (3H, m), 7.60 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.11-8.20 (3H, m), 13.30 (1H, br s). 411 409
  • TABLE 1-9
    1-65
    Figure US20200087266A1-20200319-C00285
         		1H-NMR (DMSO-D6) δ: 1.60-1.71 (6H, m), 1.79-1.83 (6H, m), 1.94-1.98 (3H, m), 4.30 (2H, d, J = 6.0 Hz), 7.42 (1H, d, J = 8.6 Hz), 7.53-7.59 (3H, m), 7.63-7.68 (2H, m), 8.10 (1H, t, J = 6.1 Hz), 8.34 (2H, d, J = 7.5 Hz), 13.34 (1H, br s). 475 473
    1-66
    Figure US20200087266A1-20200319-C00286
         		1H-NMR (DMSO-D6) δ: 1.72-1.94 (2H, m), 1.99-2.08 (2H, m), 2.10-2.20 (2H, m), 3.03- 3.11 (1H, m), 4.31 (2H, d, J = 6.0 Hz), 7.45 (1H, dd, J = 8.4, 2.0 Hz), 7.54-7.69 (5H, m), 8.28 (1H, t, J = 6.0 Hz), 8.34 (2H, d, J = 7.3 Hz), 13.34 (1H, br s). 395 393
    1-67
    Figure US20200087266A1-20200319-C00287
         		1H-NMR (DMSO-D6) δ: 1.46-1.81 (8H, m), 2.58-2.66 (1H, m), 4.32 (2H, d, J = 6.0 Hz), 7.45 (1H, dd, J = 8.4, 2.2 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.60 (1H, d, J = 8.2 Hz), 7.64-7.69 (2H, m), 8.34 (2H, d, J = 7.5 Hz), 8.40 (1H, t, J = 6.1 Hz), 13.34 (1H, br s). 409 407
    1-68
    Figure US20200087266A1-20200319-C00288
         		1H-NMR (DMSO-D6) δ: 1.50-1.68 (4H, m), 1.82-1.91 (2H, m), 2.29-2.36 (2H, m), 4.37 (2H, d, J = 5.8 Hz), 7.41 (1H, d, J = 8.8 Hz), 7.54 (2H, t, J = 7.7 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.62-7.67 (2H, m), 8.32 (2H, d, J = 7.7 Hz), 8.69 (1H, t, J = 5.9 Hz), 13.33 (1H, br s). 477 475
    1-69
    Figure US20200087266A1-20200319-C00289
         		1H-NMR (DMS0-D6) δ: 1.38 (6H, s), 4.37 (2H, d, J = 6.0 Hz), 7.44 (1H, dd, J = 8.3, 2.1 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.61 (1H, d, J = 8.4 Hz), 7.64-7.69 (2H, m), 8.34 (2H, d, J = 7.5 Hz), 8.64 (1H, t, J =6.0 Hz), 13.35 (1H, br s). 451 449
    1-70
    Figure US20200087266A1-20200319-C00290
         		1H-NMR (DMS0-D6) δ: 1.77-1.94 (2H, m), 2.30-2.39 (2H, m), 2.45-2.57 (2H, m), 4.39 (2H, d, J = 5.8 Hz), 7.44 (1H, d, J = 7.4 Hz), 7.54 (2H, t, J = 7.7 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.63-7.68 (2H, m), 8.33 (2H, d, J = 7.7 Hz), 8.78 (1H, t, J = 5.9 Hz), 13.32 (1H, br s). 463 461
    1-71
    Figure US20200087266A1-20200319-C00291
         		1H-NMR (DMSO-D6) δ: 1.08-1.42 (5H, m), 1.57-1.77 (5H, m), 2.13-2.21 (1H, m), 4.31 (2H, d, J = 6.0 Hz), 7.44 (1H, dd, J = 8.2, 2.2 Hz), 7.54-7.60 (3H, m), 7.65-7.69 (2H, m), 8.33-8.35 (3H, m), 13.34 (1H, br s). 423 421
    1-72
    Figure US20200087266A1-20200319-C00292
         		1H-NMR (DMS0-D6) δ: 1.35 (3H, s), 1.63- 1.76 (3H, m), 1.84-1.93 (1H, m), 2.30-2.37 (2H, m), 4.32 (2H, d, J = 6.2 Hz), 7.45 (1H, dd, J = 8.4, 2.0 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.64-7.68 (2H, m), 8.18 (1H, t, J = 6.1 Hz), 8.34 (2H, d, J = 7.5 Hz), 13.35 (1H, br s). 409 407
  • TABLE 1-10
    1-73
    Figure US20200087266A1-20200319-C00293
         		1H-NMR (DMSO-D6) δ: 1.19 (3H, s), 1.35- 1.43 (2H, m), 1.51-1.62 (4H, m), 1.98-2.05 (2H, m), 4.32 (2H, d, J = 6.0 Hz), 7.44 (1H, dd, J = 8.3, 2.1 Hz), 7.54-7.60 (3H, m), 7.64- 7.69 (2H, m), 8.20 (1H, t, J = 6.1 Hz), 8.34 (2H, d, J = 7.3 Hz), 13.34 (1H, br s). 423 421
    1-74
    Figure US20200087266A1-20200319-C00294
         		1H-NMR (DMSO-D6) δ: 1.12 (9H, s), 4.30 (2H, d, J = 6.0 Hz), 7.37 (1H, d, J = 8.8 Hz), 7.39 (1H, d, J = 8.8 Hz), 7.42 (1H, dd, J = 8.5, 2.0 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.62 (1H, s), 8.16 (1H, t, J = 5.9 Hz), 8.38 (1H, d, J = 8.8 Hz), 8.40 (1H, d, J = 8.8 Hz), 13.33 (1H, br s). 415 413
    1-75
    Figure US20200087266A1-20200319-C00295
         		1H-NMR (DMSO-D6) δ: 1.13 (9H, s), 3.32 (3H, s), 4.31 (2H, d, J = 6.0 Hz), 4.50 (2H, s), 7.43 (1H, d, J = 8.6 Hz), 7.53 (1H, t, J = 7.7 Hz), 7.59 (2H, d, J = 8.1 Hz), 7.63 (1H, s), 8.18 (1H, t, J = 6.0 Hz), 8.26 (1H, d, J = 7.7 Hz), 8.30 (1H, s), 13.34 (1H, br s). 441 439
    1-76
    Figure US20200087266A1-20200319-C00296
         		1H-NMR (DMSO-D6) δ: 1.14 (9H, s), 4.32 (2H, d, J = 6.0 Hz), 7.45 (1H, dd, J = 8.4, 2.1 Hz), 7.52 (1H, td, J = 8.4, 2.7 Hz), 7.59-7.63 (2H, m), 7.65 (1H, d, J = 2.1 Hz), 8.05 (1H, dt, J = 9.8, 2.0 Hz), 8.16-8.20 (2H, m), 13.43 (1H, br s). 415 413
    1-77
    Figure US20200087266A1-20200319-C00297
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 4.37 (2H, d, J = 5.8 Hz), 7.41 (1H, d, J = 8.4 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.61-7.65 (2H, m), 7.69 (1H, t, J = 7.8 Hz), 8.21 (1H, s), 8.36 (1H, d, J = 7.7 Hz), 8.63 (1H, t, J = 5.8 Hz), 13.52 (1H, br s). 535 533
    1-78
    Figure US20200087266A1-20200319-C00298
         		1H-NMR (DMSO-D6) δ: 1.48-1.71 (4H, m), 1.81-1.94 (2H, m), 2.30-2.39 (2H, m), 4.39 (2H, d, J = 5.9 Hz), 7.45 (1H, dd, J = 8.3, 1.4 Hz), 7.59-7.75 (4H, m), 8.22 (1H, br s), 8.35- 8.39 (1H, m), 8.72 (1H, t, J = 5.9 Hz), 13.51 (1H, br s). 561 559
    1-79
    Figure US20200087266A1-20200319-C00299
         		1H-NMR (DMSO-D6) δ: 1.50-1.69 (4H, m), 1.83-1.93 (2H, m), 2.29-2.39 (2H, m), 4.39 (2H, d, J = 6.0 Hz), 7.44 (1H, dd, J = 8.3, 1.9 Hz), 7.60-7.66 (2H, m), 7.82 (1H, t, J = 7.7 Hz), 8.03 (1H, d, J = 7.7 Hz), 8.59-8.65 (2H, m), 8.71 (1H, t, J = 6.0 Hz), 13.54 (1H, br s). 545 543
    1-80
    Figure US20200087266A1-20200319-C00300
         		1H-NMR (DMSO-D6) δ: 1.34 (9H, s), 1.49- 1.69 (4H, m), 1.83-1.92 (2H, m), 2.29-2.38 (2H, m), 4.38 (2H, d, J = 6.0 Hz), 7.42 (1H, d, J = 8.3 Hz), 7.49 (1H, t, J = 7.8 Hz), 7.61 (1H, d, J = 8.3 Hz), 7.65 (1H, s), 7.71 (1H, d, J = 7.8 Hz), 8.15 (1H, d, J = 7.8 Hz), 8.38 (1H, s), 8.71 (1H, t, J = 6.0 Hz), 13.35 (1H, br s). 533 531
  • TABLE 1-11
    1-81
    Figure US20200087266A1-20200319-C00301
         		1H-NMR (DMSO-D6) δ: 1.37 (6H, s), 2.32 (3H, d, J = 1.2 Hz), 4.36 (2H, d, J = 5.9 Hz), 7.50-7.43 (2H, m), 7.60 (1H, d, J = 8.4 Hz), 7.64 (1H, br s), 7.99 (1H, d, J = 10.9 Hz), 8.08 (1H, dd, J = 7.8, 1.5 Hz), 8.63 (1H, t, J = 5.9 Hz), 13.36 (1H, s). 483 481
    1-82
    Figure US20200087266A1-20200319-C00302
         		1H-NMR (DMSO-D6) δ: 1.13 (9H, s), 1.31 (6H, d, J = 6.0 Hz), 4.31 (2H, d, J = 6.0 Hz), 4.72-4.82 (1H, m), 7.06 (2H, d, J = 8.9 Hz), 7.42 (1H, d, J = 8.4 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.63 (1H, s), 8.17 (1H, t, J = 6.0 Hz), 8.29 (2H, d, J = 8.9 Hz), 13.12 (1H, br s). 455 453
    1-83
    Figure US20200087266A1-20200319-C00303
         		1H-NMR (DMSO-D6) δ: 1.17 (6H, d, J = 6.3 Hz), 1.34 (9H, s), 4.23 (2H, d, J = 6.0 Hz), 4.72-4.80 (1H, m), 7.43-7.52 (2H, m), 7.60 (1H, d, J = 8.4 Hz), 7.65-7.73 (3H, m), 8.15 (1H, d, J = 7.7 Hz), 8.38 (1H, s), 13.33 (1H, br s). 455 453
    1-84
    Figure US20200087266A1-20200319-C00304
         		1H-NMR (DMSO-D6) δ:1.27 (6H, s), 1.34 (9H, s), 3.16 (3H, s), 4.33 (2H, d, J = 6.3 Hz), 7.42-7.46 (1H, m), 7.49 (1H, d, J = 7.9 Hz), 7.57 (1H, d, J = 8.1 Hz), 7.66-7.71 (2H, m), 8.14 (1H, d, J = 7.9 Hz), 8.38 (1H, s), 8.47 (1H, t, J = 6.3 Hz), 13.32 (1H, br s). 469 467
    1-85
    Figure US20200087266A1-20200319-C00305
         		1H-NMR (DMSO-D6) δ: 1.36 (3H, t, J = 7.0 Hz), 1.51-1.69 (4H, m), 1.84-1.91 (2H, m), 2.30-2.38 (2H, m), 4.10 (2H, q, J = 7.0 Hz), 4.38 (2H, d, J = 6.0 Hz), 7.20 (1H, dd, J = 8.3, 2.4 Hz), 7.39-7.43 (1H, m), 7.46 (1H, d, J = 7.9 Hz), 7.59 (1H, d, J = 8.3 Hz), 7.63 (1H, s), 7.85 (1H, d, J = 2.4 Hz), 7.89-7.96 (1H, m), 8.71 (1H, t, J = 6.0 Hz), 13.33 (1H, br s). 521 519
    1-86
    Figure US20200087266A1-20200319-C00306
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 1.37 (6H, s), 3.73 (2H, s), 4.35 (2H, d, J = 5.8 Hz), 7.08 (2H, d, J = 9.1 Hz), 7.40 (1H, dd, J = 8.3, 2.2 Hz), 7.58 (1H, d, J = 8.3 Hz), 7.62 (1H, d, J = 1.9 Hz), 8.29 (2H, d, J = 9.1 Hz), 8.62 (1H, t, J = 5.8 Hz), 13.13 (1H, s). 537 535
    1-87
    Figure US20200087266A1-20200319-C00307
         		1H-NMR (DMSO-D6) δ: 1.54 (3H, s), 3.36 (3H, s), 4.32-4.44 (2H, m), 7.46 (1H, d, J = 8.2 Hz), 7.53-7.71 (5H, m), 8.34 (2H, d, J = 7.5 Hz), 9.04 (1H, t, J = 6.3 Hz), 13.35 (1H, br s). 467 465
    1-88
    Figure US20200087266A1-20200319-C00308
         		1H-NMR (DMSO-D6) δ: 1.00 (6H, d, J = 6.8 Hz), 1.38 (6H, s), 1.99-2.10 (1H, m), 3.83 (2H, d, J = 6.4 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.23 (1H, dd, J = 8.2, 2.6 Hz), 7.41-7.49 (2H, m), 7.61 (1H, d, J = 8.2 Hz), 7.66 (1H, s), 7.84-7.88 (1H, m), 7.93 (1H, d, J = 7.9 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.33 (1H, br s). 523 521
  • TABLE 1-12
    1-89
    Figure US20200087266A1-20200319-C00309
         		1H-NMR (DMSO-D6) δ: 1.54 (3H, s), 3.36 (3H, s), 4.32-4.44 (2H, m), 7.46 (1H, d, J = 8.2 Hz), 7.53-7.71 (5H, m), 8.34 (2H, d, J = 7.5 Hz), 9.04 (1H, t, J = 6.3 Hz), 13.35 (1H, br s). 467 465
    1-90
    Figure US20200087266A1-20200319-C00310
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 2.31 (3H, s), 4.37 (2H, d, J = 5.8 Hz), 7.31 (1H, t, J = 9.1 Hz), 7.43 (1H, dd, J = 8.4, 2.0 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.63 (1H, s), 8.21 (1H, s), 8.28 (1H, d, J = 7.4 Hz), 8.64 (1H, t, J = 5.8 Hz), 13.32 (1H, br s). 483 481
    1-91
    Figure US20200087266A1-20200319-C00311
         		1H-NMR (DMSO-D6) δ: 1.30 (6H, d, J = 6.0 Hz), 1.38 (6H, s), 4.37 (2H, d, J = 5.8 Hz), 4.66-4.72 (1H, m), 7.20 (1H, dd, J = 8.4, 2.1 Hz), 7.42-7.47 (2H, m), 7.60 (1H, d, J = 8.4 Hz), 7.66 (1H, s), 7.84-7.86 (1H, m), 7.90 (1H, d, J = 7.7 Hz), 8.64 (1H, t, J = 5.8 Hz), 13.32 (1H, br s). 509 507
    1-92
    Figure US20200087266A1-20200319-C00312
         		1H-NMR (DMSO-D6) δ: 1.34 (9H, s), 1.38 (6H, s), 4.37 (2H, d, J = 6.0 Hz), 7.42 (1H, d, J = 8.4 Hz), 7.48 (1H, t, J = 7.9 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.66 (1H, s), 7.70 (1H, d, J = 7.7 Hz), 8.15 (1H, d, J = 7.7 Hz), 8.37-8.39 (1H, m), 8.63 (1H, t, J = 6.0 Hz), 13.33 (1H, br s). 507 505
    1-93
    Figure US20200087266A1-20200319-C00313
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 2.35 (6H, s), 4.37 (2H, d, J = 5.8 Hz), 7.30 (1H, s), 7.44 (1H, d, J = 8.4 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.63 (1H, s), 7.96 (2H, s), 8.64 (1H, t, J = 5.8 Hz), 13.27 (1H, br s). 479 477
    1-94
    Figure US20200087266A1-20200319-C00314
         		1H-NMR (DMSO-D6) δ: 1.33 (9H, s), 4.55 (2H, d, J = 5.8 Hz), 7.45-7.50 (3H, m), 7.52- 7.56 (2H, m), 7.61 (1H, d, J = 8.4 Hz), 7.71 (1H, d, J = 8.4 Hz), 7.78 (1H, s), 7.89-7.91 (2H, m), 8.14 (1H, d, J = 7.7 Hz), 8.37 (1H, s), 9.15 (1H, t, J = 5.8 Hz), 13.33 (1H, br s). 473 471
    1-95
    Figure US20200087266A1-20200319-C00315
         		1H-NMR (DMSO-D6) δ: 0.88 (6H, d, J = 6.6 Hz), 1.38 (6H, s), 1.82-1.94 (1H, m), 2.55 (2H, d, J = 7.1 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.42-7.50 (3H, m), 7.59-7.67 (2H, m), 8.12- 8.20 (2H, m), 8.64 (1H, t, J = 6.0 Hz), 13.31 (1H, br s). 507 505
    1-96
    Figure US20200087266A1-20200319-C00316
         		1H-NMR (DMSO-D6) δ: 1.19 (3H, t, J = 7.1 Hz), 1.54 (3H, s), 3.46-3.54 (1H, m), 3.57- 3.66 (1H, m), 4.40 (2H, d, J = 6.2 Hz), 7.46 (1H, d, J = 8.4 Hz), 7.54-7.70 (5H, m), 8.34 (2H, d, J = 7.5 Hz), 8.86 (1H, t, J = 6.2 Hz), 13.35 (1H, br s). 481 479
  • TABLE 1-13
    1-97
    Figure US20200087266A1-20200319-C00317
         		1H-NMR (DMSO-D6) δ: 1.19 (3H, t, J = 7.1 Hz), 1.54 (3H, s), 3.46-3.54 (1H, m), 3.57- 3.66 (1H, m), 4.40 (2H, d, J = 6.2 Hz), 7.46 (1H, d, J = 8.4 Hz), 7.54-7.70 (5H, m), 8.34 (2H, d, J = 7.5 Hz), 8.86 (1H, t, J = 6.2 Hz), 13.35 (1H, br s). 481 479
    1-98
    Figure US20200087266A1-20200319-C00318
         		1H-NMR (DMSO-D6) δ: 1.24 (6H, d, J = 7.0 Hz), 1.38 (6H, s), 4.36 (2H, d, J = 5.8 Hz), 7.35-7.40 (3H, m), 7.54 (1H, d, J = 8.4 Hz), 7.61 (1H, d, J = 2.1 Hz), 8.25 (2H, d, J = 8.4 Hz), 8.62 (1H, t, J = 5.8 Hz), 13.26 (1H, br s). 493 491
    1-99
    Figure US20200087266A1-20200319-C00319
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 4.37 (2H, d, J = 5.8 Hz), 4.86 (2H, q, J = 8.8 Hz), 7.36 (1H, dd, J = 8.0, 2.4 Hz), 7.43 (1H, dd, J = 8.0, 2.4 Hz), 7.53 (1H, t, J = 8.0 Hz), 7.60 (1H, d, J = 8.0 Hz), 7.65 (1H, s), 7.93-7.95 (1H, m), 8.03 (1H, d, J = 7.7 Hz), 8.64 (1H, t, J = 5.8 Hz), 13.35 (1H, br s). 549 547
    1-100
    Figure US20200087266A1-20200319-C00320
         		1H-NMR (DMSO-D6) δ: 1.36 (6H, s), 1.79- 1.87 (1H, m), 1.93-2.05 (1H, m), 2.07-2.18 (2H, m), 2.29-2.36 (2H, m), 3.55-3.64 (1H, m), 4.35 (2H, d, J = 6.0 Hz), 7.37-7.48 (3H, m), 7.56 (1H, d, J = 8.1 Hz), 7.61 (1H, s), 8.13 (1H, d, J = 7.0 Hz), 8.18 (1H, s), 8.61 (1H, t, J = 6.0 Hz), 13.31 (1H, br s). 505 503
    1-101
    Figure US20200087266A1-20200319-C00321
         		1H-NMR (DMSO-D6) δ: 0.88 (6H, d, J = 6.5 Hz), 1.25 (9H, s), 1.84-1.95 (1H, m), 2.55 (2H, d, J = 7.2 Hz), 3.45 (2H, s), 7.35 (2H, d, J = 8.1 Hz), 7.46 (1H, d, J = 8.4 Hz), 7.57 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 7.76 (1H, s), 8.26 (2H, d, J = 8.1 Hz), 13.24 (1H, br s). 453 451
    1-102
    Figure US20200087266A1-20200319-C00322
         		1H-NMR (DMSO-D6) δ: 0.91 (3H, t, J = 7.6 Hz), 1.38 (6H, s), 1.58-1.69 (2H, m), 2.64 (2H, t, J = 7.6 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.40-7.46 (3H, m), 7.58 (1H, d, J = 8.1 Hz), 7.63 (1H, d, J = 1.9 Hz), 8.14-8.18 (2H, m), 8.63 (1H, t, J = 6.0 Hz), 13.32 (1H, br s). 493 491
    1-103
    Figure US20200087266A1-20200319-C00323
         		1H-NMR (DMSO-D6) δ: 1.54 (3H, s), 3.36 (3H, s), 4.32-4.46 (2H, m), 7.48 (1H, d, J = 7.7 Hz), 7.60-7.75 (4H, m), 8.22 (1H, s), 8.37 (1H, d, J = 7.3 Hz), 9.04 (1H, t, J = 6.3 Hz), 13.52 (1H, br s). 551 549
    1-104
    Figure US20200087266A1-20200319-C00324
         		1H-NMR (DMSO-D6) δ: 0.91 (3H, t, J = 7.4 Hz), 1.54 (3H, s), 1.58-1.69 (2H, m), 2.65 (2H, t, J = 7.6 Hz), 3.36 (3H, s), 4.32-4.45 (2H, m), 7.43-7.52 (3H, m), 7.61 (1H, d, J = 8.4 Hz), 7.67 (1H, s), 8.13-8.19 (2H, m), 9.04 (1H, t, J = 6.2 Hz), 13.31 (1H, br s). 509 507
  • TABLE 1-14
    1-105
    Figure US20200087266A1-20200319-C00325
         		1H-NMR (DMSO-D6) δ: 1.54 (3H, s), 2.34 (3H, d, J = 1.1 Hz), 3.36 (3H, s), 4.33-4.45 (2H, m), 7.44-7.52 (2H, m), 7.61 (1H, d, J = 8.2 Hz), 7.67 (1H, s), 8.00 (1H, d, J = 11.0 Hz), 8.09 (1H, dd, J = 7.9, 1.5 Hz), 9.04 (1H, t, J = 6.2 Hz), 13.38 (1H, br s). 499 497
    1-106
    Figure US20200087266A1-20200319-C00326
         		1H-NMR (DMSO-D6) δ: 1.54 (3H, s), 2.32 (3H, d, J = 1.3 Hz), 3.36 (3H, s), 4.32-4.44 (2H, m), 7.32 (1H, t, J = 9.0 Hz), 7.46 (1H, d, J = 8.3 Hz), 7.61 (1H, d, J = 8.3 Hz), 7.65 (1H, s), 8.19-8.25 (1H, m), 8.28 (1H, d, J = 7.3 Hz), 9.03 (1H, t, J = 6.3 Hz), 13.33 (1H, br s). 499 497
    1-107
    Figure US20200087266A1-20200319-C00327
         		1H-NMR (DMSO-D6) δ: 1.25 (6H, d, J = 6.9 Hz), 1.38 (6H, s), 2.94-3.06 (1H, m), 4.37 (2H, d, J = 5.9 Hz), 7.41-7.68 (5H, m), 8.13- 8.19 (1H, m), 8.22 (1H, br s), 8.64 (1H, t, J = 5.9 Hz), 13.33 (1H, br s). 493 491
    1-108
    Figure US20200087266A1-20200319-C00328
         		1H-NMR (DMSO-D6) δ: 1.25 (6H, d, J = 7.3 Hz), 1.54 (3H, s), 2.94-3.07 (1H, m), 3.36 (3H, s), 4.31-4.46 (2H, m), 7.42-7.71 (5H, m), 8.16 (1H, d, J = 7.7 Hz), 8.22 (1H, br s), 9.04 (1H, t, J = 6.0 Hz), 13.32 (1H, br s). 509 507
    1-109
    Figure US20200087266A1-20200319-C00329
         		1H-NMR (DMSO-D6) δ: 0.98 (6H, d, J = 6.5 Hz), 1.37 (6H, s), 2.07-2.00 (1H, m), 3.85 (2H, d, J = 6.5 Hz), 4.35 (2H, d, J = 6.0 Hz), 7.07 (2H, d, J = 9.1 Hz), 7.41 (1H, dd, J = 8.1, 2.1 Hz), 7.58 (1H, d, J = 8.1 Hz), 7.62 (1H, s), 8.29 (2H, d, J = 9.1 Hz), 8.63 (1H, t, J = 5.9 Hz), 13.13 (1H, br s). 523 521
    1-110
    Figure US20200087266A1-20200319-C00330
         		1H-NMR (DMSO-D6) δ: 1.22 (3H, t, J = 7.6 Hz), 1.38 (6H, s), 2.70 (2H, q, J = 7.6 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.42-7.54 (3H, m), 7.61 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 8.16 (1H, d, J = 7.7 Hz), 8.19 (1H, s), 8.65 (1H, t, J = 6.0 Hz), 13.32 (1H, br s). 479 477
    1-111
    Figure US20200087266A1-20200319-C00331
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 2.40 (3H, s), 4.37 (2H, d, J = 6.0 Hz), 7.41-7.50 (3H, m), 7.61 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 8.11-8.18 (2H, m), 8.65 (1H, t, J = 6.0 Hz), 13.32 (1H, br s). 465 463
    1-112
    Figure US20200087266A1-20200319-C00332
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 2.42 (3H, s), 4.37 (2H, d, J = 6.0 Hz), 7.44 (1H, dd, J = 8.4, 2.1 Hz), 7.61 (2H, dd, J = 8.4, 2.1 Hz), 7.64 (1H, d, J = 2.1 Hz), 8.17 (1H, dd, J = 8.4, 2.1 Hz), 8.30 (1H, d, J = 2.1 Hz), 8.65 (1H, t, J = 6.0 Hz), 13.39 (1H, br s). 499 497
  • TABLE 1-15
    1-113
    Figure US20200087266A1-20200319-C00333
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 4.36 (2H, d, J = 6.0 Hz), 4.89 (2H, q, J = 8.8 Hz), 7.20 (2H, d, J = 9.1 Hz), 7.39 (1H, dd, J = 8.3, 2.0 Hz), 7.56 (1H, d, J = 8.3 Hz), 7.62 (1H, d, J = 2.0 Hz), 8.33 (2H, d, J = 9.1 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.24 (1H, br s). 549 547
    1-114
    Figure US20200087266A1-20200319-C00334
         		1H-NMR (DMSO-D6) δ: 0.94 (6H, d, J = 6.6 Hz), 1.37 (6H, s), 1.63 (2H, q, J = 6.6 Hz), 1.79 (1H, m), 4.04 (2H, t, J = 6.6 Hz), 4.33 (2H, d, J = 6.0 Hz), 6.95 (2H, d, J = 8.9 Hz), 7.23 (1H, d, J = 8.3 Hz), 7.42 (1H, d, J = 8.3 Hz), 7.50 (1H, d, J = 2.0 Hz), 8.22 (2H, d, J = 8.9 Hz), 8.59 (1H, t, J = 6.0 Hz). 537 535
    1-115
    Figure US20200087266A1-20200319-C00335
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 2.55 (3H, s), 4.36 (2H, d, J = 6.0 Hz), 7.39 (2H, d, J = 8.7 Hz), 7.40 (1H, d, J = 8.3 Hz), 7.58 (1H, d, J = 8.3 Hz), 7.63 (1H, s), 8.26 (2H, d, J = 8.7 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.26 (1H, s). 497 495
    1-116
    Figure US20200087266A1-20200319-C00336
         		1H-NMR (DMSO-D6) δ: 1.37 (6H, s), 1.94- 1.80 (4H, m), 2.11-2.04 (2H, m), 2.77-2.69 (1H, m), 4.05 (2H, d, J = 6.7 Hz), 4.35 (2H, d, J = 5.8 Hz), 7.07 (2H, d, J = 9.1 Hz), 7.40 (1H, dd, J = 8.4, 2.1 Hz), 7.57 (1H, d, J = 8.4 Hz), 7.62 (1H, s), 8.28 (2H, d, J = 9.1 Hz), 8.62 (1H, t, J = 5.8 Hz), 13.13 (1H, s). 535 533
    1-117
    Figure US20200087266A1-20200319-C00337
         		1H-NMR (DMSO-D6) δ: 1.27 (6H, d, J = 7.1 Hz), 1.38 (6H, s), 3.19-3.28 (1H, m), 4.37 (2H, d, J = 6.0 Hz), 7.33 (1H, t, J = 9.5 Hz), 7.44 (1H, d, J = 8.4 Hz), 7.59-7.68 (2H, m), 8.21-8.26 (1H, m), 8.33 (1H, dd, J = 7.5, 2.2 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.36 (1H, br s). 511 509
    1-118
    Figure US20200087266A1-20200319-C00338
         		1H-NMR (DMSO-D6) δ: 0.92 (3H, t, J = 7.3 Hz), 1.38 (6H, s), 1.56-1.67 (2H, m), 2.67 (2H, t, J = 7.7 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.33 (1H, t, J = 9.3 Hz), 7.44 (1H, d, J = 8.4 Hz), 7.59-7.66 (2H, m), 8.21-8.30 (2H, m), 8.65 (1H, t, J = 6.0 Hz), 13.34 (1H, br s). 511 509
    1-119
    Figure US20200087266A1-20200319-C00339
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 2.01 (3H, t, J = 19.0 Hz), 4.37 (2H, d, J = 5.8 Hz), 7.44 (1H, dd, J = 8.4, 2.1 Hz), 7.61 (2H, d, J = 8.1 Hz), 7.65 (2H, d, J = 2.1 Hz), 7.74 (2H, d, J = 8.4 Hz), 8.43 (2H, d, J = 8.1 Hz), 8.64 (1H, t, J = 5.8 Hz), 13.46 (1H, br s). 515 513
    1-120
    Figure US20200087266A1-20200319-C00340
         		1H-NMR (DMSO-D6) δ: 1.01 (6H, d, J = 6.7 Hz), 1.37 (6H, s), 2.04-2.10 (1H, m), 3.95 (2H, d, J = 6.7 Hz), 4.33 (2H, d, J = 6.0 Hz), 7.25 (1H, dd, J = 8.3, 2.2 Hz), 7.29 (1H, d, J = 8.3 Hz), 7.44 (1H, d, J = 8.3 Hz), 7.51 (1H, d, J = 2.2 Hz), 8.49-8.53 (2H, m), 8.56-8.62 (1H, m). 591 589
  • TABLE 1-16
    1-121
    Figure US20200087266A1-20200319-C00341
         		1H-NMR (DMSO-D6) δ: 1.23 (3H, t, J = 7.7 Hz), 1.54 (3H, s), 2.70 (2H, q, J = 7.7 Hz), 3.36 (3H, s), 4.32-4.45 (2H, m), 7.44-7.54 (3H, m), 7.61 (1H, d, J = 8.4 Hz), 7.67 (1H, s), 8.13-8.19 (2H, m), 9.03 (1H, t, J = 6.3 Hz), 13.31 (1H, br s). 495 493
    1-122
    Figure US20200087266A1-20200319-C00342
         		1H-NMR (DMSO-D6) δ: 0.99 (3H, t, J = 7.4 Hz), 1.38 (6H, s), 1.76 (2H, m), 4.04 (2H, t, J = 6.6 Hz), 4.36 (2H, d, J = 5.8 Hz), 7.07 (2H, d, J = 8.8 Hz), 7.40 (1H, d, J = 6.0 Hz), 7.57 (1H, d, J = 6.0 Hz), 7.62 (1H, s), 8.29 (2H, d, J = 8.8 Hz), 8.63 (1H, s), 13.14 (1H, s). 509 507
    1-123
    Figure US20200087266A1-20200319-C00343
         		1H-NMR (DMSO-D6) δ: 1.00 (6H, d, J = 6.9 Hz), 1.54 (3H, s), 1.98-2.12 (1H, m), 3.36 (3H, s), 3.86 (2H, d, J = 6.2 Hz), 4.30-4.45 (2H, m), 7.09 (2H, d, J = 8.5 Hz), 7.44 (1H, d, J = 8.5 Hz), 7.59 (1H, d, J = 8.5 Hz), 7.66 (1H, br s), 8.30 (2H, d, J = 8.9 Hz), 9.03 (1H, t, J = 6.2 Hz), 13.14 (1H, br s). 539 537
    1-124
    Figure US20200087266A1-20200319-C00344
         		1H-NMR (DMSO-D6) δ: 1.21 (3H, t, J = 7.6 Hz), 1.38 (6H, s), 2.71 (2H, q, J = 7.6 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.33 (1H, t, J = 9.3 Hz), 7.44 (1H, dd, J = 8.5, 2.1 Hz), 7.59-7.66 (2H, m), 8.21-8.26 (1H, m), 8.30 (1H, dd, J = 7.7, 2.2 Hz), 8.65 (1H, t, J = 6.0 Hz), 13.35 (1H, br s). 497 495
    1-125
    Figure US20200087266A1-20200319-C00345
         		1H-NMR (DMSO-D6) δ: 1.75-2.00 (2H, m), 2.28-2.43 (5H, m), 2.45-2.61 (2H, m), 4.40 (2H, d, J = 5.7 Hz), 7.33 (1H, t, J = 9.1 Hz), 7.46 (1H, dd, J = 8.4, 1.8 Hz), 7.63 (1H, d, J 8.4 Hz), 7.66 (1H, br s), 8.19-8.32 (2H, m), 8.81 (1H, t, J = 5.7 Hz), 13.33 (1H, br s). 495 493
    1-126
    Figure US20200087266A1-20200319-C00346
         		1H-NMR (DMSO-D6) δ: 1.54 (3H, s), 3.36 (3H, s), 4.32-4.46 (2H, m), 7.48 (1H, dd, J = 8.4, 1.6 Hz), 7.63 (1H, d, J = 8.4 Hz), 7.68 (1H, br s), 7.94 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 9.04 (1H, t, J = 6.0 Hz), 13.56 (1H, br s). 535 533
    1-127
    Figure US20200087266A1-20200319-C00347
         		1H-NMR (DMSO-D6) δ: 1.20-1.39 (4H, m), 2.32 (3H, s), 4.35 (2H, d, J = 5.9 Hz), 7.33 (1H, t, J = 9.1 Hz), 7.45 (1H, dd, J = 8.4, 1.8 Hz), 7.61 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.18-8.31 (2H, m), 8.48 (1H, t, J = 5.9 Hz), 13.32 (1H, br s). 481 479
    1-128
    Figure US20200087266A1-20200319-C00348
         		1H-NMR (DMSO-D6) δ: 0.41 (2H, dd, J = 5.8, 4.0 Hz), 0.55 (2H, dd, J = 5.2, 4.0 Hz), 1.19 (3H, s), 1.38 (6H, s), 3.87 (2H, s), 4.36 (2H, d, J = 5.8 Hz), 7.07 (2H, d, J = 8.9 Hz), 7.41 (1H, d, J = 7.3 Hz), 7.58 (1H, d, J = 7.3 Hz), 7.63 (1H, s), 8.29 (2H, d, J = 8.9 Hz), 8.64 (1H, t, J = 5.8 Hz), 13.14 (1H, s). 535 533
  • TABLE 1-17
    1-129
    Figure US20200087266A1-20200319-C00349
         		1H-NMR (DMSO-D6) δ: 1.39 (6H, s), 2.43 (3H, s), 4.37 (2H, d, J = 6.0 Hz), 7.44 (1H, d, J = 8.4 Hz), 7.55 (1H, d, J = 8.4 Hz), 7.61 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.20 (1H, dd, J = 8.4, 1.5 Hz), 8.31 (1H, d, J = 1.5 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.39 (1H, br s). 499 497
    1-130
    Figure US20200087266A1-20200319-C00350
         		1H-NMR (DMSO-D6) δ: 1.30 (6H, d, J = 6.7 Hz), 1.38 (6H, s), 4.37 (2H, d, J = 5.8 Hz), 7.43 (1H, dd, J = 8.4, 2.0 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.65 (1H, d, J = 2.0 Hz), 7.83 (1H, d, J = 8.4 Hz), 8.29 (1H, d, J = 8.4 Hz), 8.52 (1H, s), 8.63 (1H, t, J = 5.8 Hz), 13.55 (1H, br s). 561 559
    1-131
    Figure US20200087266A1-20200319-C00351
         		1H-NMR (DMSO-D6) δ: 0.94 (3H, t, J = 7.3 Hz), 1.38 (6H, s), 1.45 (2H, m), 1.69-1.76 (2H, m), 3.17 (2H, d, J = 5.1 Hz), 4.08 (2H, m), 4.37 (2H, d, J = 5.8 Hz), 7.10 (2H, d, J = 9.1 Hz), 7.44 (1H, s), 7.60 (m, s), 8.31 (2H, d, J = 9.1 Hz), 8.64 (1H, s), 13.14 (1H, s). 523 521
    1-132
    Figure US20200087266A1-20200319-C00352
         		1H-NMR (DMSO-D6) δ: 1.35 (3H, t, J = 7.1 Hz), 1.37 (6H, s), 4.13 (2H, q, J = 7.1 Hz), 4.35 (2H, d, J = 6.0 Hz), 7.07 (2H, d, J = 8.8 Hz), 7.42 (aH, br s), 7.60 (2H, br s), 8.29 (2H, d, J = 8.8 Hz), 8.62 (1H, t, J = 6.0 Hz), 13.13 (1H, s). 495 493
    1-133
    Figure US20200087266A1-20200319-C00353
         		1H-NMR (DMSO-D6) δ: 0.34 (2H, m), 0.57 (2H, m), 1.23 (1H, m), 1.37 (6H, s), 3.92 (2H, d, J = 6.7 Hz), 4.35 (2H, d, J = 6.0 Hz), 7.07 (2H, s), 7.42 (1H, s), 7.59 (2H, s), 8.28 (2H, d, J = 9.1 Hz), 8.62 (1H, t, J = 6.0 Hz), 13.13 (1H, s). 521 519
    1-134
    Figure US20200087266A1-20200319-C00354
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 4.36 (2H, d, J = 5.8 Hz), 6.90 (2H, d, J = 8.8 Hz), 7.42 (1H, d, J = 8.6 Hz), 7.59 (1H, d, J = 8.6 Hz), 7.64 (1H, s), 8.23 (2H, d, J = 8.8 Hz), 8.64 (1H, t, J = 5.8 Hz), 10.46 (1H, s). 467 465
    1-135
    Figure US20200087266A1-20200319-C00355
         		1H-NMR (DMSO-D6) δ: 0.74-0.87 (2H, m), 1.01-1.09 (2H, m), 1.38 (6H, s), 2.07-2.15 (1H, m), 4.37 (2H, d, J = 6.0 Hz), 7.33 (1H, t, J = 9.3 Hz), 7.44 (1H, d, J = 8.2 Hz), 7.61 (1H, d, J = 8.2 Hz), 7.65 (1H, s), 7.96 (1H, dd, J = 7.4, 1.9 Hz), 8.15-8.21 (1H, m), 8.64 (1H, t, J = 6.0 Hz), 13.33 (1H, br s). 509 507
    1-136
    Figure US20200087266A1-20200319-C00356
         		1H-NMR (DMSO-D6) δ: 1.54 (3H, s), 2.42 (3H, s), 3.36 (3H, s), 4.32-4.45 (2H, m), 7.46 (1H, dd, J = 8.4, 2.0 Hz), 7.58-7.68 (3H, m), 8.16 (1H, dd, J = 8.4, 2.0 Hz), 8.30 (1H, d, J = 2.0 Hz), 9.04 (1H, t, J = 6.2 Hz), 13.39 (1H, br s). 515 513
  • TABLE 1-18
    1-137
    Figure US20200087266A1-20200319-C00357
         		1H-NMR (DMSO-D6) δ: 0.99 (3H, t, J = 7.4 Hz), 1.54 (3H, s), 1.71-1.81 (2H, m), 3.36 (3H, s), 4.04 (2H, t, J = 6.6 Hz), 4.32- 4.43 (2H, m), 7.07 (2H, d, J = 9.0 Hz), 7.42 (1H, dd, J = 8.3, 1.9 Hz), 7.58 (1H, d, J = 8.3 Hz), 7.65 (1H, d, J = 1.9 Hz), 8.29 (2H, d, J = 9.0 Hz), 9.03 (1H, t, J = 6.6 Hz), 13.15 (1H, br s). 525 523
    1-138
    Figure US20200087266A1-20200319-C00358
         		1H-NMR (DMSO-D6) δ: 1.54 (3H, s), 2.01 (3H, t, J = 19.0 Hz), 3.36 (3H, s, J = 8.8 Hz), 4.38 (2H, dd, J = 6.3, 2.8 Hz), 7.44 (1H, dd, J = 8.4, 2.1 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.66 (1H, d, J = 2.1 Hz), 7.73 (2H, d, J = 8.4 Hz), 8.42 (2H, d, J = 8.4 Hz), 9.03 (1H, t, J = 6.3 Hz), 13.47 (1H, s). 531 529
    1-139
    Figure US20200087266A1-20200319-C00359
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 3.86 (3H, s), 4.37 (2H, d, J = 6.0 Hz), 7.10 (2H, d, J = 8.8 Hz), 7.42 (1H, d, J = 8.6 Hz), 7.59 (1H, d, J = 8.6 Hz), 7.64 (1H, s), 8.32 (2H, d, J = 8.8 Hz), 8.63 (1H, t, J = 6.0 Hz), 13.15 (1H, s). 481 479
    1-140
    Figure US20200087266A1-20200319-C00360
         		1H-NMR (DMSO-D6) δ: 1.31 (6H, d, J = 6.0 Hz), 1.38 (6H, s), 4.37 (2H, d, J = 5.8 Hz), 4.77 (1H, m), 7.06 (2H, d, J = 8.8 Hz), 7.41 (1H, d, J = 8.4 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.29 (2H, d, J = 8.8 Hz), 8.63 (1H, t, J = 5.8 Hz), 13.13 (1H, s). 509 507
    1-141
    Figure US20200087266A1-20200319-C00361
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 1.66 (2H, m), 1.85 (2H, m), 2.07 (2H, m), 4.36 (2H, d, J = 5.8 Hz), 4.81 (1H,m), 6.98 (2H, d, J = 8.8 Hz), 7.40 (1H, d, J = 7.9 Hz), 7.57 (1H, d, J = 7.9 Hz), 7.62 (1H, s), 8.28 (2H, d, J = 8.8 Hz), 8.63 (1H, t, J = 5.8 Hz), 13.14 (1H, s). 521 519
    1-142
    Figure US20200087266A1-20200319-C00362
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 2.83 (2H, td, J = 11.3, 5.5 Hz), 4.33 (2H, t, J = 5.8 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.12 (2H, d, J = 8.8 Hz), 7.43 (1H, s), 7.58 (1H, s), 7.64 (1H, s), 8.32 (2H, d, J = 8.8 Hz), 8.64 (1H, d, J = 6.0 Hz), 13.18 (1H, s). 563 561
    1-143
    Figure US20200087266A1-20200319-C00363
         		1H-NMR (DMSO-D6) δ: 1.38 (3H, s), 1.39 (6H, s), 4.18 (2H, s), 4.32 (2H, d, J = 5.8 Hz), 4.37 (2H, d, J = 5.8 Hz), 4.51 (2H, d, J = 5.8 Hz), 7.15 (2H, d, J = 8.8 Hz), 7.43 (1H, d, J = 7.7 Hz), 7.60 (1H, d, J = 7.7 Hz), 7.64 (1H, s), 8.33 (2H, d, J = 8.8 Hz), 8.64 (1H, t, J = 5.8 Hz), 13.17 (1H, s). 551 549
    1-144
    Figure US20200087266A1-20200319-C00364
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 3.32 (3H, s), 3.69 (2H, t, J = 4.4 Hz), 4.21 (2H, t, J = 4.4 Hz), 4.37 (2H, d, J = 5.8 Hz), 7.11 (2H, d, J = 8.8 Hz), 7.42 (1H, d, J = 8.1 Hz), 7.60 (1H, d, J = 8.1 Hz), 7.64 (1H, s), 8.31 (2H, d, J = 8.8 Hz), 8.63 (1H, d, J = 5.8 Hz), 13.15 (1H, s). 525 523
  • TABLE 1-19
    1-145
    Figure US20200087266A1-20200319-C00365
         		1H-NMR (DMSO-D6) δ: 0.91 (3H, t, J = 7.6 Hz), 1.38 (6H, s), 1.80 (2H, q, J = 7.6 Hz), 4.21 (2H, s), 4.35 (2H, d, J = 6.0 Hz), 4.37 (2H, d, J = 5.8 Hz), 4.46 (2H, d, J = 6.0 Hz), 7.15 (2H, d, J = 8.8 Hz), 7.42 (1H, d, J = 8.4 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.32 (2H, d, J = 8.8 Hz), 8.64 (1H, t, J =5.8 Hz), 13.17 (1H, s). 565 563
    1-146
    Figure US20200087266A1-20200319-C00366
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 1.84 (3H, t, J = 2.2 Hz), 4.37 (2H, d, J = 5.8 Hz), 4.87 (2H, d, J = 2.2 Hz), 7.12 (2H, d, J = 8.8 Hz), 7.43 (1H, d, J = 8.4 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 8.32 (2H, d, J = 8.8 Hz), 8.64 (1H, t, J = 5.8 Hz), 13.17 (1H, s). 519 517
    1-147
    Figure US20200087266A1-20200319-C00367
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 2.53 (3H, s), 4.37 (2H, d, J = 6.0 Hz), 7.42 (1H, dd, J = 8.1, 2.1 Hz), 7.59 (1H, d, J = 8.1 Hz), 7.63 (1H, d, J = 2.1 Hz), 7.84 (1H, d, J = 8.1 Hz), 8.30 (1H, d, J = 8.1 Hz), 8.34 (1H, s), 8.64 (1H, t, J = 6.0 Hz), 13.53 (1H, br s). 533 531
    1-148
    Figure US20200087266A1-20200319-C00368
         		1H-NMR (DMSO-D6) δ: 1.54 (3H, s), 2.54 (3H, s), 3.36 (3H, s), 4.33-4.44 (2H, m), 7.47 (1H, dd, J = 8.3, 2.0 Hz), 7.62 (1H, d, J = 8.3 Hz), 7.66 (1H, d, J = 2.0 Hz), 7.86 (1H, d, J = 8.3 Hz), 8.31 (1H, d, J = 8.3 Hz), 8.35 (1H, s), 9.04 (1H, t, J = 6.4 Hz), 13.53 (1H, br s). 549 547
    1-149
    Figure US20200087266A1-20200319-C00369
         		1H-NMR (DMSO-D6) δ: 1.22-1.36 (4H, m), 2.53 (3H, s), 4.34 (2H, d, J = 6.0 Hz), 7.40 (1H, dd, J = 8.4, 2.1 Hz), 7.57 (1H, d, J = 8.4 Hz), 7.61 (1H, d, J = 2.1 Hz), 7.82 (1H, d, J = 8.4 Hz), 8.30 (1H, d, J = 8.4Hz), 8.34 (1H, s), 8.47 (1H, t, J = 6.0 Hz), 13.53 (1H, br s). 531 529
    1-150
    Figure US20200087266A1-20200319-C00370
         		1H-NMR (DMSO-D6) δ: 1.38 (6H, s), 2.31 (3H, s), 2.32 (3H, s), 4.37 (2H, d, J = 6.0 Hz), 7.33 (1H, d, J = 8.2 Hz), 7.43 (1H, d, J = 8.2 Hz), 7.58-7.67 (2H, m), 8.07 (1H, d, J = 8.2 Hz), 8.12 (1H, s), 8.65 (1H, t, J = 6.0 Hz), 13.23 (1H, br s). 479 477
    1-151
    Figure US20200087266A1-20200319-C00371
         		1H-NMR (DMSO-D6) δ: 1.21 (3H, t, J = 7.5 Hz), 1.54 (3H, s), 2.71 (2H, q, J = 7.5 Hz), 3.36 (3H, s), 4.32-4.45 (2H, m), 7.33 (1H, t, J = 9.3 Hz), 7.46 (1H, dd, J = 8.4, 2.0 Hz), 7.61 (1H, d, J = 8.4 Hz), 7.66 (1H, s), 8.20-8.26 (1H, m), 8.29 (1H, dd, J = 7.6, 2.3 Hz), 9.04 (1H, t, J = 6.3 Hz), 13.35 (1H, br s). 513 511
    1-152
    Figure US20200087266A1-20200319-C00372
         		1H-NMR (DMSO-D6) δ: 1.19-1.27 (5H, m), 1.32-1.37 (2H, m), 2.71 (2H, q, J = 7.5 Hz), 4.35 (2H, d, J = 6.0 Hz), 7.33 (1H, t, J = 9.2 Hz), 7.45 (1H, dd, J = 8.4, 2.0 Hz), 7.61 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 8.21-8.26 (1H, m), 8.30 (1H, dd, J = 7.5, 2.0 Hz), 8.48 (1H, t, J = 6.0 Hz), 13.34 (1H, br s). 495 493
  • TABLE 1-20
    1-153
    Figure US20200087266A1-20200319-C00373
         		1H-NMR (DMSO-D6) δ: 1.39 (6H, s), 4.37 (2H, d, J = 6.0 Hz), 7.45 (1H, dd, J = 8.3, 2.1 Hz), 7.59-7.66 (3H, m), 8.32-8.38 (1H, m), 8.45 (1H, dd, J = 7.4, 2.1 Hz), 8.65 (1H, t, J = 6.0 Hz), 13.48 (0H, br s). 503 501
    1-154
    Figure US20200087266A1-20200319-C00374
         		1H-NMR (DMSO-D6) δ: 0.99 (3H, t, J = 7.4 Hz), 1.22-1.27 (2H, m), 1.31-1.37 (2H, m), 1.71-1.81 (2H, m), 4.04 (2H, t, J = 6.5 Hz), 4.34 (2H, d, J = 6.0 Hz), 7.09 (2H, d, J = 9.0 Hz), 7.43 (1H, dd, J = 8.4, 2.0 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.31 (2H, d, J = 9.0 Hz), 8.47 (1H, t, J = 6.0 Hz), 13.14 (1H, br s). 507 505
    1-155
    Figure US20200087266A1-20200319-C00375
         		1H-NMR (DMSO-D6) δ: 0.90 (3H, t, J = 7.3 Hz), 1.27 (3H, d, J = 6.0 Hz), 1.37 (6H,s), 1.43 (2H, m), 1.52-1.60 (1H, m), 1.67 (1H, m), 4.37 (2H, d, J = 5.8 Hz), 4.62 (1H, m), 7.07 (2H, d, J = 9.1 Hz), 7.42 (1H, d, J = 8.1 Hz), 7.59 (1H, d, J = 8.1 Hz), 7.64 (1H, s), 8.29 (2H, d, J = 9.1 Hz), 8.63 (1H, t, J = 5.8 Hz), 13.13 (1H, s). 537 535
    1-156
    Figure US20200087266A1-20200319-C00376
         		1H-NMR (DMSO-D6) δ: 0.90 (3H, t, J = 7.3 Hz), 1.27 (3H, d, J = 6.0 Hz), 1.36 (6H, s), 1.43 (2H, m), 1.51-1.60 (1H, m), 1.65 (1H, m), 4.37 (2H, d, J = 5.8 Hz), 4.62 (1H, m), 7.06 (2H, d, J = 8.8 Hz), 7.41 (1H, d, J = 8.1 Hz), 7.59 (1H, d, J = 8.1 Hz), 7.64 (1H, s), 8.29 (2H, d, J = 8.8 Hz), 8.63 (1H, t, J = 5.8 Hz), 13.13 (1H, s). 537 535
    1-157
    Figure US20200087266A1-20200319-C00377
         		1H-NMR (DMSO-D6) δ: 0.77-0.83 (2H, m), 1.02-1.08 (2H, m), 1.22-1.27 (2H, m), 1.31-1.36 (2H, m), 2.07-2.15 (1H, m), 4.34 (2H, d, J = 5.8 Hz), 7.33 (1H, t, J = 9.3 Hz), 7.44 (1H, d, J = 8.4 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 7.96 (1H, d, J = 7.9 Hz), 8.15-8.20 (1H, m), 8.47 (1H, t, J = 5.8 Hz), 13.31 (1H, br s). 507 505
    1-158
    Figure US20200087266A1-20200319-C00378
         		1H-NMR (DMSO-D6) δ: 0.99 (3H, t, J = 7.5 Hz), 1.21 (6H, s), 1.76 (2H,m), 3.03 (2H, s), 4.04 (2H, t, J = 6.5 Hz), 4.40 (2H, s), 7.09 (2H, d, J = 8.9 Hz), 7.47 (1H, d, J = 8.3 Hz), 7.65 (1H, d, J = 8.3 Hz), 7.67 (1H, s), 8.31 (2H, d, J = 8.9 Hz), 13.14 (1H, s). 453 451
    1-159
    Figure US20200087266A1-20200319-C00379
         		1H-NMR (DMSO-D6) δ: 0.72-0.77 (2H, m), 0.97-1.04 (2H, m), 1.38 (6H, s), 1.99-2.07 (1H, m), 4.37 (2H, d, J = 6.0 Hz), 7.34-7.45 (3H, m), 7.59 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 8.03 (1H, s), 8.10 (1H, d, J = 7.7 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.32 (1H, br s). 491 489
    1-160
    Figure US20200087266A1-20200319-C00380
         		1H-NMR (DMSO-D6) δ: 0.72-0.78 (2H, m), 0.98-1.04 (2H, m), 1.21-1.27 (2H, m), 1.31- 1.36 (2H, m), 2.00-2.07 (1H, m), 4.34 (2H, d, J = 5.8 Hz), 7.35-7.46 (3H, m), 7.60 (1H, d, J = 8.1 Hz), 7.66 (1H, s), 8.03 (1H, s), 8.10 (1H, d, J = 7.4 Hz), 8.47 (1H, t, J = 5.8 Hz), 13.30 (1H, br s). 489 487
  • TABLE 1-21
    1-161
    Figure US20200087266A1-20200319-C00381
         		1H-NMR (DMSO-D6) δ: 1.32-1.38 (9H, m), 2.55 (3H, s), 4.10 (2H, q, J = 7.2 Hz), 4.35 (2H, d, J = 5.8 Hz), 6.86-6.92 (2H, m), 7.39 (1H, d, J = 8.6 Hz), 7.56 (1H, d, J = 8.6 Hz), 7.62 (1H, s), 7.81 (1H, br s), 8.61 (1H, t, J = 5.8 Hz), 13.08 (1H, br s). 509 507
    1-162
    Figure US20200087266A1-20200319-C00382
         		1H-NMR (DMSO-D6) δ: 0.35 (2H, q, J = 5.1 Hz), 0.57-0.60 (2H, m), 1.22-1.30 (1H, m), 1.54 (3H, s), 3.36 (3H, s), 3.93 (2H, d, J = 7.0 Hz), 4.37 (2H, dd, J = 5.9, 2.9 Hz), 7.07 (2H, d, J = 8.8 Hz), 7.42 (1H, d, J = 8.4 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 8.29 (2H, d, J = 8.8 Hz), 9.02 (1H, t, J = 5.9 Hz), 13.14 (1H, br s). 537 535
    1-163
    Figure US20200087266A1-20200319-C00383
         		1H-NMR (DMSO-D6) δ: 0.33-0.37 (2H, m), 0.57-0.61 (2H, m), 1.22-1.35 (5H, m), 3.92 (2H, d, J = 7.0 Hz), 4.33 (2H, d, J = 5.8 Hz), 7.05 (2H, d, J = 8.8 Hz), 7.39 (1H, dd, J = 8.3, 1.9 Hz), 7.55 (1H, d, J = 8.3 Hz), 7.61 (1H, d, J = 1.9 Hz), 8.28 (2H, d, J = 8.8 Hz), 8.46 (1H, t, J = 5.8 Hz), 13.15 (1H, br s). 519 517
    1-164
    Figure US20200087266A1-20200319-C00384
         		1H-NMR (DMSO-D6) δ: 1.05 (2H, m), 1.10 (2H, m), 1.38 (6H, s), 4.20 (2H, s), 4.34 (2H, d, J = 5.6 Hz), 7.02 (2H, d, J = 7.9 Hz), 7.32 (1H, d, J = 7.5 Hz), 7.49 (1H, d, J = 7.5 Hz), 7.56 (1H, s), 8.26 (2H, d, J = 7.9 Hz), 8.61 (1H, t, J = 5.6 Hz), 13.18 (1H, s). 589 587
    1-165
    Figure US20200087266A1-20200319-C00385
         		1H-NMR (DMSO-D6) δ: 0.77-0.82 (2H, m), 1.01-1.07 (2H, m), 1.54 (3H, s), 2.07-2.15 (1H, m), 3.35 (3H, s), 4.31-4.44 (2H, m), 7.32 (1H, t, J = 9.3 Hz), 7.44 (1H, d, J = 8.4 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.67 (1H, s), 7.95 (1H, d, J = 7.2 Hz), 8.14-8.19 (1H, m), 9.02 (1H, t, J = 6.3 Hz), 13.31 (1H, br s). 525 523
    1-166
    Figure US20200087266A1-20200319-C00386
         		1H-NMR (DMSO-D6) δ: 0.33-0.38 (2H, m), 0.56-0.62 (2H, m), 1.19-1.31 (7H, m), 2.59 (2H, q, J = 12.1 Hz), 3.93 (2H, d, J = 7.1 Hz), 4.33 (2H, d, J = 6.0 Hz), 7.07 (2H, d, J = 9.0 Hz), 7.42 (1H, d, J = 8.4 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.63 (1H, s), 8.29 (2H, d, J = 9.0 Hz), 8.35 (1H, t, J = 6.0 Hz), 13.14 (1H, br s). 535 533
    1-167
    Figure US20200087266A1-20200319-C00387
         		1H-NMR (DMSO-D6) δ: 0.11-0.16 (2H, m), 0.41-0.48 (2H, m), 0.79-0.90 (1H, m), 1.38 (6H, s), 1.65 (2H, q, J = 6.5 Hz), 4.14 (2H, t, J = 6.5 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.09 (2H, d, J = 9.0 Hz), 7.41 (1H, dd, J = 8.4, 2.0 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.64 (1H, d, J = 2.0 Hz), 8.30 (2H, d, J = 9.0 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.15 (1H, br s). 535 533
    1-168
    Figure US20200087266A1-20200319-C00388
         		1H-NMR (DMSO-D6) δ: 0.32-0.38 (2H, m), 0.56-0.62 (2H, m), 1.01 (3H, s), 1.12 (3H, s), 1.19-1.30 (1H, m), 1.78-1.85 (2H, m), 1.90- 1.97 (2H, m), 2.95-3.05 (1H, m), 3.93 (2H, d, J = 7.0 Hz), 4.30 (2H, d, J = 6.0 Hz), 7.08 (2H, d, J = 8.6 Hz), 7.43 (1H, d, J = 8.4 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.62 (1H, s), 8.25- 8.32 (3H, m), 13.13 (1H, br s). 493 491
  • TABLE 1-22
    1-169
    Figure US20200087266A1-20200319-C00389
         		1H-NMR (DMSO-D6) δ: 0.32-0.38 (2H, m), 0.55-0.62 (2H, m), 1.20-1.29 (1H, m), 1.74 (3H, s), 3.93 (2H, d, J = 6.7 Hz), 4.43 (2H, d, J = 6.0 Hz), 7.07 (2H, d, J = 8.4 Hz), 7.41 (1H, d, J = 8.4 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 8.29 (2H, d, J = 8.4 Hz), 9.10 (1H, t, J = 6.0 Hz), 13.14 (1H, br s). 575 573
    1-170
    Figure US20200087266A1-20200319-C00390
         		1H-NMR (DMSO-D6) δ: 0.33-0.38 (2H, m), 0.56-0.62 (2H, m), 1.20-1.31 (1H, m), 1.57- 1.90 (6H, m), 1.97-2.09 (2H, m), 2.29-2.39 (1H, m), 3.93 (2H, d, J = 7.1 Hz), 4.31-4.33 (2H, br m), 7.08 (2H, d, J = 8.8 Hz), 7.44 (1H, d, J = 8.4 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.29 (2H, d, J = 8.8 Hz), 8.50 (1H, t, J J = 6.0 Hz), 13.14 (1H, br s). 529 527
    1-171
    Figure US20200087266A1-20200319-C00391
         		1H-NMR (DMSO-D6) δ: 0.33-0.38 (2H, m), 0.56-0.62 (2H, m), 0.79 (6H, t, J = 7.5 Hz), 1.20-1.54 (5H, m), 1.98-2.07 (1H, m), 3.94 (2H, d, J = 7.1 Hz), 4.34 (2H, d, J = 6.0 Hz), 7.08 (2H, d, J = 9.0 Hz), 7.46 (1H, d, J = 8.2 Hz), 7.60 (1H, d, J = 8.2 Hz), 7.66 (1H, s), 8.29 (2H, d, J = 9.0 Hz), 8.46 (1H, t, J = 5.7 Hz), 13.14 (1H, br s). 481 479
    1-172
    Figure US20200087266A1-20200319-C00392
         		1H-NMR (DMSO-D6) δ: 0.99-1.33 (5H, m), 1.38 (6H, s), 1.62-1.86 (6H, m), 3.89 (2H, d, J = 6.2 Hz), 4.36 (2H, d, J = 6.0 Hz), 7.06 (2H, d, J = 9.0 Hz), 7.40 (1H, dd, J = 8.4, 2.0 Hz), 7.57 (1H, d, J = 8.4 Hz), 7.62 (1H, d, J = 2.0 Hz), 8.29 (2H, d, J = 9.0 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.15 (1H, br s). 563 561
    1-173
    Figure US20200087266A1-20200319-C00393
         		1H-NMR (DMS0-D6) δ: 0.33-0.37 (2H, m), 0.56-0.62 (2H, m), 1.11 (6H, s), 1.17 (6H, s), 1.20-1.29 (2H, m), 3.93 (2H, d, J = 7.1 Hz), 4.29 (2H, d, J = 6.0 Hz), 7.07 (2H, d, J = 8.8 Hz), 7.43 (1H, dd, J = 8.4, 2.0 Hz), 7.57 (1H, d, J = 8.4 Hz), 7.62 (1H, d, J = 2.0 Hz), 8.29 (2H, d, J = 8.8 Hz), 8.37 (1H, t, J = 6.0 Hz), 13.14 (1H, br s). 507 505
    1-174
    Figure US20200087266A1-20200319-C00394
         		1H-NMR (DMSO-D6) δ: 2.51 (3H, s), 7.49- 7.46 (3H, m), 7.63-7.60 (2H, m), 7.66 (1H, d, J = 7.9 Hz), 7.77 (2H, d, J = 8.6 Hz), 7.96 (1H, d, J = 7.9 Hz), 8.22 (1H, s), 8.53 (2H, d, J = 8.6 Hz), 13.46 (1H, s). 389 387
    1-175
    Figure US20200087266A1-20200319-C00395
         		1H-NMR (DMSO-D6) δ: 0.36 (2H, td, J = 5.2, 3.9 Hz), 0.56-0.60 (2H, m), 1.23-1.30 (1H, m), 1.38 (6H, s), 2.21 (3H, s), 3.91 (2H, d, J = 6.7 Hz), 4.34 (2H, d, J = 5.8 Hz), 6.97 (1H, d, J = 8.6 Hz), 7.29 (1H, dd, J = 8.3, 1.9 Hz), 7.47 (1H, d, J = 8.4 Hz), 7.53 (1H, d, J = 1.9 Hz), 8.08-8.14 (2H, m), 8.61 (1H, t, J = 5.8 Hz). 535 533
  • TABLE 1-23
    1-176
    Figure US20200087266A1-20200319-C00396
         		1H-NMR (DMSO-D6) δ: 0.30 (2H, td, J = 5.3, 4.0 Hz), 0.56 (2H, ddd, J = 9.2, 5.3, 3.1 Hz), 1.22-1.27 (1H, m), 1.38 (6H, s), 2.29 (6H, s), 3.69 (2H, d, J = 7.0 Hz), 4.36 (2H, d, J = 5.8 Hz), 7.41 (1H, dd, J = 8.4, 1.9 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.61 (1H, d, J = 1.9 Hz), 8.02 (2H, s), 8.64 (1H, t, J = 5.8 Hz), 13.18 (1H, br s). 549 547
    1-177
    Figure US20200087266A1-20200319-C00397
         		1H-NMR (DMSO-D 6) δ: 0.88 (6H, d, J = 6.4 Hz), 1.54 (3H, s), 1.83-1.98 (1H, m), 2.55 (2H, d, J = 7.3 Hz), 3.36 (3H, s), 4.31-4.45 (2H, m), 7.35 (2H, d, J = 8.3 Hz), 7.45 (1H, dd, J = 8.1, 2.0 Hz), 7.60 (1H, d, J = 8.1 Hz), 7.67 (1H, br s), 8.26 (2H, d, J = 8.3 Hz), 9.03 (1H, t, J = 6.2 Hz), 13.26 (1H, br s). 523 521
    1-178
    Figure US20200087266A1-20200319-C00398
         		1H-NMR (DMSO-D6) δ: 0.99 (6H, d, J = 6.4 Hz), 1.20-1.39 (4H, m), 1.96-2.13 (1H, m), 3.84 (2H, d, J = 6.4 Hz), 4.33 (2H, d, J = 6.0 Hz), 7.05 (2H, d, J = 9.1 Hz), 7.37 (1H, dd, J = 8.3, 2.0 Hz), 7.54 (1H, d, J = 8.3 Hz), 7.60 (1H, d, J = 2.0 Hz), 8.28 (2H, d, J = 9.1 Hz), 8.46 (1H, t, J = 5.8 Hz), 13.15 (1H, br s). 521 519
    1-179
    Figure US20200087266A1-20200319-C00399
         		1H-NMR (DMSO-D6) δ: 0.94 (3H, s), 0.95 (3H, s), 1.27-1.36 (2H, m), 1.38 (6H, s), 1.41- 1.49 (2H, m), 1.56-1.66 (2H, m), 1.80-1.89 (2H, m), 4.36 (2H, d, J = 5.7 Hz), 4.45-4.53 (1H, m), 7.07 (2H, d, J = 9.0 Hz), 7.40 (1H, dd, J = 8.2, 2.0 Hz), 7.58 (1H, d, J = 8.2 Hz), 7.63 (1H, d, J = 2.0 Hz), 8.28 (2H, d, J = 9.0 Hz), 8.63 (1H, t, J = 6.1 Hz), 13.13 (1H, br s). 577 575
    1-180
    Figure US20200087266A1-20200319-C00400
         		1H-NMR (DMSO-D6) δ: 0.91 (3H, t, J = 7.3 Hz), 1.27-1.37 (2H, m), 1.38 (6H, s), 1.55- 1.64 (2H, m), 2.67 (2H, t, J = 7.7 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.38 (2H, d, J = 8.2 Hz), 7.42 (1H, d, J = 8.4 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.25 (2H, d, J = 8.2 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.27 (1H, br s). 507 505
    1-181
    Figure US20200087266A1-20200319-C00401
         		1H-NMR (DMSO-D6) δ: 0.90 (3H, t, J = 7.7 Hz), 1.26-1.37 (2H, m), 1.54 (3H, s), 1.55- 1.64 (2H, m), 2.67 (2H, t, J = 7.7 Hz), 3.36 (3H, s), 4.32-4.44 (2H, m), 7.38 (2H, d, J = 7.5 Hz), 7.44 (1H, d, J = 8.4 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.66 (1H, s), 8.25 (2H, d, J = 7.5 Hz), 9.03 (1H, t, J = 6.2 Hz), 13.27 (1H, br s). 523 521
    1-182
    Figure US20200087266A1-20200319-C00402
         		1H-NMR (DMSO-D6) δ: 0.99 (6H, t, J = 6.0 Hz), 1.37 (6H, s), 2.00-2.10 (1H, m), 4.11 (2H, d, J = 6.5 Hz), 4.34 (2H, d, J = 6.0 Hz), 6.88 (1H, d, J = 8.7 Hz), 7.30 (1H, dd, J = 8.1, 2.1 Hz), 7.48 (1H, d, J = 8.1 Hz), 7.57 (1H, d, J = 2.1 Hz), 8.47 (1H, dd, J = 8.7, 2.4 Hz), 8.60 (1H, t, J = 6.0 Hz), 9.02 (1H, d, J = 2.4 Hz). 524 522
    1-183
    Figure US20200087266A1-20200319-C00403
         		1H-NMR (DMSO-D6) δ: 1.24 (6H, s), 2.32 (3H, d, J = 1.3 Hz), 2.59 (2H, q, J = 12.1 Hz), 4.34 (2H, d, J = 6.2 Hz), 7.32 (1H, t, J = 9.2 Hz), 7.44 (1H, dd, J = 8.4, 2.0 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.63 (1H, d, J = 2.0 Hz), 8.19- 8.25 (1H, m), 8.28 (1H, d, J = 7.7 Hz), 8.35 (1H, t, J = 5.8 Hz), 13.33 (1H, br s). 497 495
  • TABLE 1-24
    1-184
    Figure US20200087266A1-20200319-C00404
         		1H-NMR (DMSO-D6) δ: 0.29-0.39 (2H, m), 0.47-0.55 (2H, m), 1.08-1.17 (1H, m), 1.32 (3H, d, J = 6.3 Hz), 1.38 (6H, s), 4.10-4.18 (1H, m), 4.36 (2H, d, J = 6.0 Hz), 7.05 (2H, d, J = 8.8 Hz), 7.41 (1H, d, J = 8.0 Hz), 7.58 (1H, d, J = 8.0 Hz), 7.63 (1H, s), 8.27 (2H, d, J = 8.8 Hz), 8.63 (1H, t, J = 6.0 Hz), 13.13 (1H, br s). 535 533
    1-185
    Figure US20200087266A1-20200319-C00405
         		1H-NMR (DMSO-D6) δ: 0.29-0.39 (2H, m), 0.47-0.55 (2H, m), 1.08-1.17 (1H, m), 1.32 (3H, d, J = 6.3 Hz), 1.38 (6H, s), 4.10-4.18 (1H, m), 4.36 (2H, d, J = 6.0 Hz), 7.05 (2H, d, J = 8.8 Hz), 7.41 (1H, d, J = 8.0 Hz), 7.58 (1H, d, J = 8.0 Hz), 7.63 (1H, s), 8.27 (2H, d, J = 8.8 Hz), 8.63 (1H, t, J = 6.0 Hz), 13.13 (1H, br s). 535 533
    1-186
    Figure US20200087266A1-20200319-C00406
         		1H-NMR (DMSO-D6) δ: 1.37 (6H, s), 2.28 (3H, d, J = 1.2 Hz), 4.33 (2H, d, J = 6.0 Hz), 7.15 (1H, t, J = 9.1 Hz), 7.22 (1H, dd, J = 8.4, 2.3 Hz), 7.41 (1H, d, J = 8.4 Hz), 7.49 (1H, d, J = 2.3 Hz), 8.10-8.16 (1H, m), 8.19 (1H, dd, J = 8.1, 1.6 Hz), 8.59 (1H, t, J = 6.0 Hz). 505 503
    1-187
    Figure US20200087266A1-20200319-C00407
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 3.75 (2H, s), 4.57 (2H, d, J = 5.7 Hz), 5.42 (1H, t, J = 5.7 Hz), 7.10 (2H, d, J = 8.8 Hz), 7.53 (1H, d, J = 8.4 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.71 (1H, s), 8.31 (2H, d, J = 8.8 Hz), 13.13 (1H, br s). 400 398
    1-188
    Figure US20200087266A1-20200319-C00408
         		1H-NMR (DMSO-D6) δ: 1.45 (3H, d, J = 6.8 Hz), 1.49 (3H, s), 2.32 (3H, s), 3.33 (3H, s), 5.02-5.10 (1H, m), 7.32 (1H, t, J = 9.3 Hz), 7.60 (2H, s), 7.76 (1H, s), 8.18-8.24 (1H, m), 8.27 (1H, d, J = 6.8 Hz), 8.81 (1H, d, J = 8.4 Hz), 13.32 (1H, s). 513 511 1
    1-189
    Figure US20200087266A1-20200319-C00409
         		1H-NMR (DMSO-D6) δ: 1.46 (3H, d, J = 6.8 Hz), 1.54 (3H, s), 2.31 (3H, s), 3.37 (3H, s), 5.02-5.11 (1H, m), 7.32 (1H, t, J = 9.2 Hz), 7.54 (1H, d, J = 8.4 Hz), 7.61 (1H, d, J = 8.4 Hz), 7.72 (1H, s), 8.19-8.25 (1H, m), 8.28 (1H, d, J = 7.3 Hz), 8.83 (1H, d, J = 8.2 Hz), 13.33 (1H, br s). 513 511 1
    1-190
    Figure US20200087266A1-20200319-C00410
         		1H-NMR (DMSO-D6) δ: 0.33-0.37 (2H, m), 0.56-0.62 (2H, m), 1.21-1.30 (1H, m), 2.85 (3H, br s), 2.90 (3H, br s), 3.94 (2H, d, J = 7.1 Hz), 5.13 (2H, s), 7.09 (2H, d, J = 8.6 Hz), 7.56-7.67 (2H, br m), 7.78 (1H, br s), 8.30 (2H, d, J = 8.6 Hz), 13.15 (1H, br s). 455 453
    1-191
    Figure US20200087266A1-20200319-C00411
         		1H-NMR (DMSO-D6) δ: 1.30-1.40 (9H, m), 1.44-1.55 (3H, m), 1.64-1.74 (2H, m), 1.80- 1.88 (2H, m), 2.63-2.70 (1H, m), 4.33 (2H, d, J = 6.0 Hz), 7.25 (1H, dd, J = 8.6, 2.1 Hz), 7.40-7.46 (3H, m), 7.52 (1H, d, J = 2.1 Hz), 8.25 (2H, d, J = 8.1 Hz), 8.59 (1H, t, J = 6.0 Hz). 557 555
  • TABLE 1-25
    1-192
    Figure US20200087266A1-20200319-C00412
         		1H-NMR (DMSO-D6) δ: 0.35 (2H, dt, J = 7.9, 2.8 Hz), 0.57-0.61 (2H, m), 1.21-1.30 (1H, m), 3.94 (2H, d, J = 7.0 Hz), 7.09 (2H, d, J = 8.8 Hz), 7.77 (1H, d, J = 8.4 Hz), 8.09 (1H, dd, J = 8.4, 1.6 Hz), 8.30 (2H, d, J = 9.0 Hz), 8.33 (1H, s), 13.31 (1H, s). 398 396
    1-193
    Figure US20200087266A1-20200319-C00413
         		1H-NMR (DMSO-D6) δ: 0.35 (2H, td, J = 5.2, 4.1 Hz), 0.56-0.62 (2H, m), 1.21-1.29 (1H, m), 3.94 (2H, d, J = 7.0 Hz), 7.08 (2H, d, J = 8.8 Hz), 7.58 (1H, s), 7.74 (1H, d, J = 8.4 Hz), 8.06 (1H, d, J = 8.4 Hz), 8.17 (1H, s), 8.27 (1H, d, J = 2.1 Hz), 8.31 (2H, d, J = 8.8 Hz), 13.14 (1H, s). 397 395
    1-194
    Figure US20200087266A1-20200319-C00414
         		1H-NMR (DMSO-D6) δ: 0.35 (2H, q, J = 4.8 Hz), 0.57-0.61 (2H, m), 1.22-1.29 (1H, m), 2.81 (3H, d, J = 4.4 Hz), 3.94 (2H, d, J = 7.0 Hz), 7.09 (2H, d, J = 8.8 Hz), 7.75 (1H, d, J = 8.4 Hz), 8.02 (1H, d, J = 8.1 Hz), 8.24 (1H, s), 8.31 (2H, d, J = 8.8 Hz), 8.63-8.67 (1H, m), 13.19 (1H, s). 411 409
    1-195
    Figure US20200087266A1-20200319-C00415
         		1H-NMR (DMSO-D6) δ: 0.33-0.37 (2H, m), 0.57-0.61 (2H, m), 1.20-1.30 (1H, m), 2.95 (3H, s), 3.00 (3H, s), 3.94 (2H, d, J = 7.0 Hz), 7.09 (2H, d, J = 8.8 Hz), 7.63 (1H, d, J = 9.1 Hz), 7.70 (1H, d, J = 8.1 Hz), 7.83 (1H, d, J = 1.9 Hz), 8.30 (2H, d, J = 9.1 Hz), 13.16 (1H, s). 425 423
    1-196
    Figure US20200087266A1-20200319-C00416
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 1.51 (6H, s), 3.05 (3H, s), 3.75 (2H, s), 4.65 (2H, s), 7.10 (2H, d, J = 8.8 Hz), 7.41 (1H, d, J = 8.6 Hz), 7.61-7.66 (2H, m), 8.31 (2H, d, J = 8.8 Hz), 13.15 (1H, br s). 551 549
    1-197
    Figure US20200087266A1-20200319-C00417
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 3.34 (3H, s), 3.75 (2H, s), 4.49 (2H, s), 7.10 (2H, d, J = 8.8 Hz), 7.53 (1H, dd, J = 8.2, 2.0 Hz), 7.62 (1H, d, J = 8.2 Hz), 7.72 (1H, d, J = 2.0 Hz), 8.31 (2H, d, J = 8.8 Hz), 13.14 (1H, br s). 414 412
    1-198
    Figure US20200087266A1-20200319-C00418
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 2.79 (2H, t, J = 6.6 Hz), 3.65 (2H, td, J = 6.6, 5.1 Hz), 3.75 (2H, s), 4.69 (1H, t, J = 5.1 Hz), 7.10 (2H, d, J = 8.8 Hz), 7.47 (1H, s), 7.54 (1H, d, J = 8.1 Hz), 7.63 (1H, s), 8.31 (2H, d, J = 8.8 Hz), 13.10 (1H, br s). 414 412
    1-199
    Figure US20200087266A1-20200319-C00419
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 2.89 (2H, t, J = 6.5 Hz), 3.25 (3H, s), 3.58 (2H, t, J = 6.5 Hz), 3.75 (2H, s), 7.10 (2H, d, J = 8.8 Hz), 7.47 (1H, d, J = 8.1 Hz), 7.55 (1H, d, J = 8.1 Hz), 7.65 (1H, d, J = 1.9 Hz), 8.31 (2H, d, J = 8.8 Hz), 13.10 (1H, s). 428 426
  • TABLE 1-26
    1-200
    Figure US20200087266A1-20200319-C00420
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 1.45 (3H, d, J = 7.1 Hz), 1.49 (3H, s), 3.33 (3H, s), 3.75 (2H, s), 5.02-5.11 (1H, m), 7.09 (2H, d, J = 8.8 Hz), 7.58 (2H, s), 7.76 (1H, s), 8.29 (2H, d, J = 8.8 Hz), 8.82 (1H, d, J = 8.2 Hz), 13.14 (1H, br s). 567 565 2
    1-201
    Figure US20200087266A1-20200319-C00421
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 1.47 (3H, d, J = 7.1 Hz), 1.54 (3H, s), 3.37 (3H, s), 3.75 (2H, s), 5.02-5.11 (1H, m), 7.09 (2H, d, J = 8.2 Hz), 7.51 (1H, d, J = 8.4 Hz), 7.59 (1H, J = 8.4 Hz), 7.72 (1H, s), 8.31 (2H, d, J = 8.2 Hz), 8.83 (1H, d, J = 8.2 Hz), 13.15 (1H, br s). 567 565 2
    1-202
    Figure US20200087266A1-20200319-C00422
         		1H-NMR (DMSO-D6) δ: 1.48 (6H, s), 4.29 (2H, d, J = 6.0 Hz), 7.08 (1H, t, J = 7.6 Hz), 7.21 (2H, t, J = 7.6 Hz), 7.30 (2H, d, J = 8.4 Hz), 7.33-7.41 (2H, m), 7.52-7.60 (3H, m), 7.68 (1H, t, J = 7.2 Hz), 8.03 (1H, t, J = 6.0 Hz), 8.34 (2H, d, J = 8.1 Hz), 13.33 (1H, br s). 459 457
    1-203
    Figure US20200087266A1-20200319-C00423
         		1H-NMR (DMSO-D6) δ: 1.00 (9H, t, J = 16.5 Hz), 1.29 (6H, s), 2.80 (2H, t, J = 7.0 Hz), 3.30 (4H, s), 3.34 (3H, q, J = 6.7 Hz), 3.73 (2H, s), 7.07 (2H, d, J = 8.8 Hz), 7.37 (1H, dd, J = 8.4, 2.0 Hz), 7.51 (1H, d, J = 8.4 Hz), 7.57 (1H, d, J = 2.1 Hz), 8.00 (1H, t, J = 5.6 Hz), 8.29 (2H, d, J = 8.8 Hz), 13.10 (1H, br s). 551 549
    1-204
    Figure US20200087266A1-20200319-C00424
         		1H-NMR (DMSO-D6) δ: 0.89 (6H, d, J = 6.7 Hz), 1.02 (9H, s), 1.81-1.91 (1H, m), 3.25 (2H, d, J = 6.5 Hz), 3.75 (2H, s), 4.53 (2H, s), 7.10 (2H, d, J = 8.8 Hz), 7.54 (1H, d, J = 8.4 Hz), 7.62 (1H, d, J = 8.1 Hz), 7.73 (1H, s), 8.31 (2H, d, J = 8.8 Hz), 13.12 (1H, s). 456 454
    1-205
    Figure US20200087266A1-20200319-C00425
         		1H-NMR (DMSO-D6) δ: 0.91 (3H, t, J = 7.3 Hz), 1.02 (9H, s), 1.28-1.37 (2H, m), 1.55- 1.62 (2H, m), 2.65 (2H, t, J = 7.7 Hz), 3.75 (2H, s), 7.10 (2H, d, J = 9.1 Hz), 7.43 (1H, d, J = 8.1 Hz), 7.53 (1H, d, J = 8.1 Hz), 7.61 (1H, d, J = 2.1 Hz), 8.31 (2H, d, J = 9.1 Hz), 13.09 (1H, s). 426 424
    1-206
    Figure US20200087266A1-20200319-C00426
         		1H-NMR (DMSO-D6) δ: 0.88 (6H, d, J = 6.6 Hz), 1.51 (6H, s), 1.84-1.95 (1H, m), 2.55 (2H, d, J = 7.3 Hz), 3.05 (3H, s), 4.65 (2H, s), 7.35 (2H, d, J = 8.4 Hz), 7.41 (1H, d, J = 8.2 Hz), 7.62-7.66 (2H, m), 8.26 (2H, d, J = 8.4 Hz), 13.26 (1H, br s). 521 519
    1-207
    Figure US20200087266A1-20200319-C00427
         		1H-NMR (DMSO-D6) δ: 1.01 (9H, s), 3.75 (2H, s), 4.67 (2H, d, J = 5.5 Hz), 5.55 (1H, t, J = 5.5 Hz), 7.10 (2H, d, J = 8.8 Hz), 7.73 (1H, d, J = 7.9 Hz), 7.78 (1H, s), 7.90 (1H, d, J = 7.9 Hz), 8.29 (2H, d, J = 8.8 Hz), 13.21 (1H, br s). 434 432
  • TABLE 1-27
    1-208
    Figure US20200087266A1-20200319-C00428
         		1H-NMR (DMSO-D6) δ: 1.01 (9H, s), 1.39 (6H, s), 3.74 (2H, s), 4.44 (2H, d, J = 6.0 Hz), 7.08 (2H, d, J = 8.6 Hz), 7.57 (1H, d, J = 8.1 Hz), 7.68 (1H, s), 7.88 (1H, d, J = 8.1 Hz), 8.28 (2H, d, J = 8.6 Hz), 8.69 (1H, t, J = 6.0 Hz), 13.23 (1H, br s). 571 569
    1-209
    Figure US20200087266A1-20200319-C00429
         		1H-NMR (CDCl3) δ: 1.07 (9H, s), 1.12 (3H, t, J = 5.8 Hz), 1.48 (9H, br s), 3.27 (2H, br s), 3.70 (2H, s), 4.48 (2H, s), 7.02 (2H, d, J = 8.6 Hz), 7.41 (1H, br s), 7.50 (1H, d, J = 8.1 Hz), 7.89 (1H, s), 8.50 (2H, d, J = 8.4 Hz). 527 525
    1-210
    Figure US20200087266A1-20200319-C00430
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 1.23 (3H, t, J = 7.2 Hz), 2.99 (2H, dt, J = 19.7, 7.2 Hz), 3.76 (2H, s), 4.23 (2H, t, J = 6.0 Hz), 7.13 (2H, d, J = 9.1 Hz), 7.74 (2H, s), 7.97 (1H, s), 8.33 (2H, d, J = 9.1 Hz), 9.05 (2H, s). 427 425
    1-211
    Figure US20200087266A1-20200319-C00431
         		1H-NMR (DMSO-D6) δ: 1.00-1.32 (5H, m), 1.51 (6H, s), 1.61-1.85 (6H, m), 3.05 (3H, s), 3.89 (2H, d, J = 6.2 Hz), 4.65 (2H, s), 7.08 (2H, d, J = 9.0 Hz), 7.40 (1H, d, J = 8.2 Hz), 7.61-7.65 (2H, m), 8.30 (2H, d, J = 9.0 Hz), 13.14 (1H, br s). 577 575
    1-212
    Figure US20200087266A1-20200319-C00432
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 3.24 (2H, q, J = 9.5 Hz), 3.75 (2H, s), 3.86 (2H, s), 7.10 (2H, d, J = 8.6 Hz), 7.54-7.63 (2H, m), 7.74 (1H, s), 8.32 (2H, d, J = 8.6 Hz), 13.11 (1H, s). 481 479
    1-213
    Figure US20200087266A1-20200319-C00433
         		1H-NMR (DMSO-D6) δ: 1.30-1.41 (3H, m), 1.44-1.56 (9H, m), 1.65-1.73 (2H, m), 1.81- 1.88 (2H, m), 2.66-2.73 (1H, m), 3.05 (3H, s), 4.65 (2H, s), 7.42 (1H, d, J = 8.6 Hz), 7.54 (2H, d, J = 8.4 Hz), 7.62-7.66 (2H, m), 8.30 (2H, d, J = 8.4 Hz), 13.36 (1H, br s). 571 569
    1-214
    Figure US20200087266A1-20200319-C00434
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 1.09 (6H, s), 2.72 (2H, s), 3.75 (2H, s), 4.40 (1H, s), 7.10 (2H, d, J = 8.8 Hz), 7.44 (1H, d, J = 8.8 Hz), 7.53 (1H, d, J = 8.1 Hz), 7.61 (1H, s), 8.31 (2H, d, J = 8.8 Hz), 13.10 (1H, br s). 442 440
    1-215
    Figure US20200087266A1-20200319-C00435
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 1.10 (6H, s), 2.81 (2H, s), 3.17 (3H, s), 3.75 (2H, s), 7.10 (2H, d, J = 8.8 Hz), 7.41 (1H, dd, J = 8.5, 1.7 Hz), 7.53 (1H, d, J = 8.5 Hz), 7.58 (1H, d, J = 1.7 Hz), 8.31 (2H, d, J = 8.8 Hz), 13.10 (1H, s). 456 454
  • TABLE 1-28
    1-216
    Figure US20200087266A1-20200319-C00436
         		1H-NMR (DMSO-D6) δ: 1.00 (9H, s), 1.06 (3H, d, J = 6.3 Hz), 2.68 (2H, d, J = 6.0 Hz), 3.73 (2H, s), 3.82-3.88 (1H, m), 4.60 (1H, d, J = 4.9 Hz), 7.09 (2H, d, J = 9.1 Hz), 7.42 (1H, d, J = 9.3 Hz), 7.51 (1H, d, J = 8.1 Hz), 7.59 (1H, s), 8.30 (2H, d, J = 9.1 Hz), 13.08 (1H, s). 428 426
    1-217
    Figure US20200087266A1-20200319-C00437
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 1.20 (9H, s), 2.36 (2H, t, J = 7.6 Hz), 2.86 (2H, t, J = 7.6 Hz), 3.75 (2H, s), 7.10 (2H, d, J = 8.8 Hz), 7.40-7.46 (2H, m), 7.53 (1H, d, J = 8.2 Hz), 7.61 (1H, s), 8.31 (2H, d, J = 8.8 Hz), 13.11 (1H, br s). 497 495
    1-218
    Figure US20200087266A1-20200319-C00438
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 2.66 (2H, t, J = 7.6 Hz), 2.81 (3H, s), 2.87 (2H, t, J = 7.6 Hz), 2.95 (3H, s), 3.75 (2H, s), 7.10 (2H, d, J = 8.8 Hz), 7.46-7.56 (2H, m), 7.66 (1H, d, J = 2.0 Hz), 8.31 (2H, d, J = 9.0 Hz), 13.11 (1H, br s). 469 467
    1-219
    Figure US20200087266A1-20200319-C00439
         		1H-NMR (DMSO-D6) δ: 0.89 (6H, d, J = 6.5 Hz), 1.30-1.40 (3H, m), 1.46-1.55 (3H, m), 1.65-1.73 (2H, m), 1.80-1.90 (3H, m), 2.67- 2.72 (1H, m), 3.25 (2H, d, J = 6.5 Hz), 4.53 (2H, s), 7.54 (2H, d, J = 8.4 Hz), 7.57 (1H, d, J = 1.6 Hz), 7.64 (1H, d, J = 8.1 Hz), 7.73 (1H, s), 8.31 (2H, d, J = 8.4 Hz), 13.33 (1H, br s). 476 474
    1-220
    Figure US20200087266A1-20200319-C00440
         		1H-NMR (DMSO-D6) δ: 1.51 (6H, s), 3.06 (3H, s), 4.65 (2H, s), 7.42 (1H, d, J = 8.4 Hz), 7.56 (2H, t, J = 7.7 Hz), 7.62-7.69 (3H, m), 8.34 (2H, d, J = 7.7 Hz), 13.34 (1H, br s). 465 463
    1-221
    Figure US20200087266A1-20200319-C00441
         		1H-NMR (DMSO-D6) δ: 1.24 (9H, s), 3.02 (3H, s), 4.62 (2H, s), 7.40 (1H, dd, J = 8.4, 1.8 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.61-7.69 (3H, 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s). 465 463
    1-222
    Figure US20200087266A1-20200319-C00442
         		1H-NMR (DMSO-D6) δ: 1.01 (1.8H, d, J = 6.7 Hz), 1.04 (4.2H, d, J = 6.7 Hz), 2.84 (0.9H, s), 2.84-2.88 (0.3H, m), 2.90-2.97 (0.7H, m), 3.01 (2.1H, s), 4.57 (1.4H, s), 4.71 (0.6H, s), 7.42 (1H, dd, J = 8.3, 1.9 Hz), 7.57 (2H, t, J = 7.6 Hz), 7.60-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s). 397 395
    1-223
    Figure US20200087266A1-20200319-C00443
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 1.37 (9H, s), 2.79 (3H, s), 3.75 (2H, s), 4.50 (2H, s), 7.11 (2H, d, J = 8.9 Hz), 7.55 (1H, dd, J = 8.1, 1.6 Hz), 7.68 (1H, d, J = 8.1 Hz), 7.72 (1H, s), 8.31 (2H, d, J = 8.9 Hz), 13.17 (1H, br s). 533 531
  • TABLE 1-29
    1-224
    Figure US20200087266A1-20200319-C00444
         		1H-NMR (DMSO-D6) δ: 0.97-1.05 (3H, m), 2.34-2.43 (2H, m), 2.85 (0.9H, s), 2.96 (2.1H, s), 4.57 (1.4H, s), 4.65 (0.6H, s), 7.44 (1H, d, J = 8.4 Hz), 7.54-7.69 (5H, m), 8.34 (2H, d, J = 8.4 Hz), 13.35 (1H, br s). 383 381
    1-225
    Figure US20200087266A1-20200319-C00445
         		1H-NMR (DMSO-D6) δ: 0.84-0.93 (3H, m), 1.50-1.60 (2H, m), 2.31-2.38 (2H, m), 2.84 (0.9H, s), 2.96 (2.1H, s), 4.57 (1.4H, s), 4.66 (0.6H, s), 7.41-7.46 (1H, m), 7.54-7.69 (5H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s). 397 395
    1-226
    Figure US20200087266A1-20200319-C00446
         		1H-NMR (DMSO-D6) δ: 0.87-0.93 (6H, m), 1.99-2.10 (1H, m), 2.22-2.28 (2H, m), 2.84 (0.9H, s), 2.97 (2.1H, s), 4.58 (1.4H, s), 4.66 (0.6H, s), 7.40-7.46 (1H, m), 7.56 (2H, t, J = 7.6 Hz), 7.60-7.70 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s). 411 409
    1-227
    Figure US20200087266A1-20200319-C00447
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 1.43 (6H, s), 2.88 (2H, t, J = 7.5 Hz), 3.03 (3H, s), 3.55 (2H, t, J = 7.5 Hz), 3.74 (2H, s), 7.09 (2H, d, J = 9.0 Hz), 7.45 (1H, dd, J = 8.0, 2.0 Hz), 7.55 (1H, d, J = 8.0 Hz), 7.64 (1H, d, J = 2.0 Hz), 8.30 (2H, d, J = 9.0 Hz), 13.11 (1H, br s). 565 563
    1-228
    Figure US20200087266A1-20200319-C00448
         		1H-NMR (DMSO-D6) δ: 1.46-1.86 (8H, m), 2.83 (0.9H, s), 2.96-3.10 (1H, m), 3.01 (2.1H, s), 4.57 (1.4H, s), 4.72 (0.6H, s), 7.42 (1H, d, J = 8.3 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.60-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.33 (1H, br s). 423 421
    1-229
    Figure US20200087266A1-20200319-C00449
         		1H-NMR (DMSO-D6) δ: 1.10-1.43 (5H, m), 1.58-1.77 (5H, m), 2.56-2.68 (1H, m), 2.81 (0.9H, s), 3.01 (2.1H, s), 4.56 (1.4H, s), 4.70 (0.6H, s), 7.38-7.43 (1H, m), 7.56 (2H, t, J = 7.6 Hz), 7.59-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s). 437 435
    1-230
    Figure US20200087266A1-20200319-C00450
         		1H-NMR (DMSO-D6) δ: 2.87-2.97 (3H, m), 4.54 (0.8H, s), 4.75 (1.2H, s), 7.39-7.51 (5.4H, m), 7.54-7.61 (2.6H, m), 7.62-7.70 (2.4H, m), 7.74-7.83 (0.6H, m), 8.35 (2H, d, J = 7.6 Hz), 13.35 (1H, br s). 431 429
    1-231
    Figure US20200087266A1-20200319-C00451
         		1H-NMR (DMSO-D6) δ: 0.79 (3H, t, J = 7.4 Hz), 1.19 (6H, s), 1.64 (2H, q, J = 7.4 Hz), 3.02 (3H, s), 4.61 (2H, s), 7.43 (1H, d, J = 8.3 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.61-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.33 (1H, br s). 425 423
  • TABLE 1-30
    1-232
    Figure US20200087266A1-20200319-C00452
         		1H-NMR (DMSO-D6) δ: 0.88 (6H, d, J = 6.7 Hz), 1.35 (9H, s), 1.84-1.95 (1H, m), 2.55 (2H, d, J = 7.4 Hz), 2.90 (3H, s), 3.77 (2H, s), 7.35 (2H, d, J = 8.1 Hz), 7.41 (1H, d, J = 8.3 Hz), 7.57 (1H, d, J = 8.3 Hz), 7.62 (1H, s), 8.26 (2H, d, J = 8.1 Hz), 13.24 (1H, br s). 467 465
    1-233
    Figure US20200087266A1-20200319-C00453
         		1H-NMR (DMSO-D6) δ: 0.75-0.84 (6H, m), 1.33-1.46 (2H, m), 1.47-1.60 (2H, m), 2.62- 2.71 (1H, m), 2.88 (0.9H, s), 3.03 (2.1H, s), 4.61 (1.4H, s), 4.72 (0.6H, s), 7.41-7.47 (1H, m), 7.56 (2H, t, J = 7.6 Hz), 7.61-7.69 (3H, m), 8.33 (2H, d, J = 7.6 Hz), 13.35 (1H, br s). 425 423
    1-234
    Figure US20200087266A1-20200319-C00454
         		1H-NMR (DMSO-D6) δ: 1.37 (6H, s), 2.78 (0.6H, br s), 3.14 (3H, s), 3.23 (2.4H, br s), 4.59 (1.6H, s), 5.09 (0.4H, br s), 7.45 (1H, d, J = 7.9 Hz), 7.56 (2H, t, J = 7.9 Hz), 7.61- 7.69 (3H, m), 8.33 (2H, d, J = 7.9 Hz), 13.33 (1H, br s). 427 425
    1-235
    Figure US20200087266A1-20200319-C00455
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 1.12 (3H, t, J = 6.8 Hz), 1.50 (6H, s), 3.42 (2H, br s), 3.75 (2H, s), 4.66 (2H, s), 7.09 (2H, d, J = 8.8 Hz), 7.40 (1H, d, J = 8.3 Hz), 7.61 (1H, d, J = 8.3 Hz), 7.62 (1H, s), 8.30 (2H, d, J = 8.8 Hz), 13.14 (1H, s). 565 563
    1-236
    Figure US20200087266A1-20200319-C00456
         		1H-NMR (DMSO-D6) δ: 1.21 (6H, s), 3.04 (2H, s), 4.41 (2H, s), 7.48 (1H, d, J = 8.6 Hz), 7.57 (2H, t, J = 7.6 Hz), 7.64-7.71 (3H, m), 8.35 (2H, d, J = 7.6 Hz), 13.34 (1H, br s). 395 393
    1-237
    Figure US20200087266A1-20200319-C00457
         		1H-NMR (DMSO-D6) δ: 1.48 (6H, s), 2.45 (3H, br s), 4.55 (2H, br s), 7.18 (1H, t, J = 7.2 Hz), 7.22-7.26 (2H, m), 7.28-7.34 (2H, m), 7.41 (1H, br s), 7.53-7.68 (5H, m), 8.35 (2H, d, J = 8.1 Hz), 13.34 (1H, br s). 473 471
    1-238
    Figure US20200087266A1-20200319-C00458
         		1H-NMR (DMSO-D6) δ: 1.56-1.67 (4H, m), 2.08-2.17 (2H, m), 2.37-2.46 (2H, m), 3.02 (3H, s), 4.65 (2H, s), 7.39 (1H, d, J = 8.3 Hz), (2H, t, J = 8.0 Hz), 7.60-7.68 (3H, m), 8.34 (2H, d, J = 8.0 Hz), 13.34 (1H, br s). 491 489
    1-239
    Figure US20200087266A1-20200319-C00459
         		1H-NMR (DMSO-D6) δ: 1.23 (6H, s), 3.02 (3H, s), 3.20 (3H, s), 3.43 (2H, s), 4.63 (2H, s), 7.42 (1H, d, J = 8.3 Hz), 7.54-7.70 (5H, m), 8.34 (2H, d, J = 7.6 Hz), 13.36 (1H, br s). 441 439
  • TABLE 1-31
    1-240
    Figure US20200087266A1-20200319-C00460
         		1H-NMR (DMSO-D6) δ: 1.07 (6H, s), 1.83 (2H, t, J = 6.9 Hz), 3.21 (2H, t, J = 6.9 Hz), 4.44 (2H, s), 7.42 (1H, d, J = 8.3 Hz), 7.57 (2H, t, J = 7.6 Hz), 7.62-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s). 409 407
    1-241
    Figure US20200087266A1-20200319-C00461
         		1H-NMR (DMSO-D6) δ: 1.15 (6H, s), 1.64- 1.69 (2H, m), 1.74-1.81 (2H, m), 3.26 (2H, t, J = 6.0 Hz), 4.53 (2H, s), 7.42 (1H, d, J = 8.1 Hz), 7.56 (2H, t, J = 7.4 Hz), 7.60-7.69 (3H, m), 8.35 (2H, d, J = 7.4 Hz), 13.33 (1H, br s). 423 421
    1-242
    Figure US20200087266A1-20200319-C00462
         		1H-NMR (DMSO-D6) δ: 1.61 (3H, s), 2.82 (0.6H, br s), 3.23 (2.4H, s), 3.37 (3H, s), 4.64 (1.6H, s), 4.87-5.12 (0.4H, m), 7.46 (1H, d, J = 8.3 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.63-7.71 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s). 481 479
    1-243
    Figure US20200087266A1-20200319-C00463
         		1H-NMR (DMSO-D6) δ: 1.61 (3H, s), 2.82 (0.6H, br s), 3.23 (2.4H, s), 3.37 (3H, s), 4.64 (1.6H, s), 4.87-5.12 (0.4H, m), 7.46 (1H, d, J = 8.3 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.63-7.71 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s). 481 479
    1-244
    Figure US20200087266A1-20200319-C00464
         		1H-NMR (DMSO-D6) δ: 1.18-1.31 (3H, m), 1.46-1.59 (3H, m), 1.63-1.72 (2H, m), 2.51- 2.58 (2H, m), 3.15 (3H, s), 4.71 (2H, s), 7.43 (1H, dd, J = 8.1, 1.8 Hz), 7.56 (2H, t, J = 7.5 Hz), 7.63-7.69 (3H, m), 8.34 (2H, d, J = 7.5 Hz), 13.36 (1H, br s). 505 503
    1-245
    Figure US20200087266A1-20200319-C00465
         		1H-NMR (DMSO-D6) δ: 0.81 (3H, t, J = 7.4 Hz), 1.15-1.25 (8H, m), 1.54-1.60 (2H, m), 3.02 (3H, s), 4.61 (2H, s), 7.42 (1H, d, J = 8.6 Hz), 7.56 (2H, t, J = 7.4 Hz), 7.60-7.69 (3H, m), 8.34 (2H, d, J = 7.4 Hz), 13.34 (1H, br s). 439 437
    1-246
    Figure US20200087266A1-20200319-C00466
         		1H-NMR (DMSO-D6) δ: 2.88 (0.9H, s), 3.04- 3.22 (6.1H, m), 3.63-3.77 (1H, m), 4.62 (1.4H, s), 4.80 (0.6H, s), 7.42-7.48 (1H, m), 7.52- 7.58 (2H, m), 7.60-7.69 (3H, m), 8.34 (2H, d, J = 7.9 Hz), 13.35 (1H, br s). 471 469
    1-247
    Figure US20200087266A1-20200319-C00467
         		1H-NMR (DMSO-D6) δ: 1.14-1.36 (5H, m), 1.38-1.50 (3H, m), 1.89-1.97 (2H, m), 3.37 (2H, s), 4.36 (2H, d, J = 6.0 Hz), 4.75 (1H, br s), 7.49 (1H, d, J = 8.3 Hz), 7.53-7.59 (3H, m), 7.64-7.70 (2H, m), 8.09 (1H, t, J = 6.0 Hz), 8.34 (2H, d, J = 7.4 Hz), 13.32 (1H, br s). 453 451
  • TABLE 1-32
    1-248
    Figure US20200087266A1-20200319-C00468
         		1H-NMR (DMSO-D6) δ: 1.26-1.37 (3H, m), 1.42-1.50 (1H, m), 1.59-1.71 (6H, m), 3.02 (2H, s), 4.40 (2H, s), 7.47 (1H, dd, J = 8.1, 2.1 Hz), 7.56 (2H, t, J = 7.9 Hz), 7.63-7.69 (3H, m), 8.34 (2H, d, J = 7.9 Hz), 13.33 (1H, br s). 435 433
    1-249
    Figure US20200087266A1-20200319-C00469
         		1H-NMR (DMSO-D6) δ: 1.21 (6H, s), 2.94 (2H, s), 3.11 (3H, s), 4.63 (2H, s), 7.06-7.10 (2H, m), 7.11-7.16 (1H, m), 7.16-7.22 (2H, m), 7.39 (1H, dd, J = 7.9, 1.8 Hz), 7.55 (2H, t, J = 7.4 Hz), 7.59-7.67 (3H, m), 8.34 (2H, d, J = 7.4 Hz), 13.35 (1H, br s). 453 451
    1-250
    Figure US20200087266A1-20200319-C00470
         		1H-NMR (DMSO-D6) δ: 0.71 (6H, t, J = 7.4 Hz), 1.51 (4H, q, J = 7.4 Hz), 3.47 (2H, d, J = 4.0 Hz), 4.34 (2H, d, J = 5.7 Hz), 4.62 (1H, br s), 7.48 (1H, d, J = 7.7 Hz), 7.53-7.61 (3H, m), 7.64-7.71 (2H, m), 8.11 (1H, t, J = 5.7 Hz), 8.34 (2H, d, J = 7.3 Hz), 13.34 (1H, br s). 441 439
    1-251
    Figure US20200087266A1-20200319-C00471
         		1H-NMR (DMSO-D6) δ: 0.87 (6H, t, J = 7.4 Hz), 1.52-1.63 (4H, m), 3.01 (2H, s), 4.40 (2H, s), 7.49 (1H, d, J = 8.3 Hz), 7.57 (2H, t, J = 7.9 Hz), 7.64-7.72 (3H, m), 8.34 (2H, d, J = 7.9 Hz), 13.35 (1H, br s). 423 421
    1-252
    Figure US20200087266A1-20200319-C00472
         		1H-NMR (DMSO-D6) δ: 1.02 (0.6H, br s), 1.17 (2.4H, t, J = 6.9 Hz), 1.62 (3H, s), 2.81 (0.6H, s), 3.25 (2.4H, s), 3.54-3.67 (2H, m), 4.63 (1.6H, s), 4.90-5.17 (0.4H, m), 7.45 (1H, dd, J = 8.2, 1.8 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.63-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s). 495 493
    1-253
    Figure US20200087266A1-20200319-C00473
         		1H-NMR (DMSO-D6) δ: 1.02 (0.6H, br s), 1.17 (2.4H, t, J = 6.9 Hz), 1.62 (3H, s), 2.81 (0.6H, s), 3.25 (2.4H, s), 3.54-3.67 (2H, m), 4.63 (1.6H, s), 4.90-5.17 (0.4H, m), 7.45 (1H, dd, J = 8.2, 1.8 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.63-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s). 495 493
    1-254
    Figure US20200087266A1-20200319-C00474
         		1H-NMR (DMSO-D6) δ: 1.51-1.55 (6H, m), 1.86-1.95 (2H, m), 3.46 (2H, s), 4.35 (2H, d, J = 6.0 Hz), 4.99 (1H, br s), 7.47 (1H, dd, J = 8.4, 2.0 Hz), 7.54-7.60 (3H, m), 7.64-7.69 (2H, m), 8.12 (1H, t, J = 6.0 Hz), 8.34 (2H, d, J = 7.7 Hz), 13.34 (1H, br s). 439 437
    1-255
    Figure US20200087266A1-20200319-C00475
         		1H-NMR (DMSO-D6) δ: 1.53-1.68 (4H, m), 1.77-1.89 (4H, m), 3.17 (2H, s), 4.42 (2H, s), 7.47 (1H, dd, J = 8.3, 2.1 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.64-7.69 (3H, m), 8.35 (2H, d, J = 7.6 Hz). 421 419
  • TABLE 1-33
    1-256
    Figure US20200087266A1-20200319-C00476
         		1H-NMR (DMSO-D6) δ: 0.69 (3H, d, J = 6.2 Hz), 0.85-0.98 (2H, m), 1.06-1.28 (3H, m), 1.42-1.50 (2H, m), 2.05-2.12 (2H, m), 3.28 (2H, s), 4.36 (2H, d, J = 6.0 Hz), 4.73 (1H, br s), 7.49 (1H, d, J = 8.1 Hz), 7.53-7.59 (3H, m), 7.63-7.69 (2H, m), 8.10 (1H, t, J = 6.0 Hz), 8.34 (2H, d, J = 7.9 Hz), 13.34 (1H, br s). 467 465 3
    1-257
    Figure US20200087266A1-20200319-C00477
         		1H-NMR (DMSO-D6) δ: 0.89 (3H, d, J = 5.5 Hz), 1.33-1.46 (3H, m), 1.51-1.62 (4H, m), 1.87-1.94 (2H, m), 2.94 (2H, s), 4.39 (2H, s), 7.47 (1H, dd, J = 8.3, 2.3 Hz), 7.56 (2H, t, J = 7.9 Hz), 7.63-7.69 (3H, m), 8.34 (2H, d, J = 7.9 Hz), 13.33 (1H, br s). 449 447 3
    1-258
    Figure US20200087266A1-20200319-C00478
         		1H-NMR (DMSO-D6) δ: 1.34 (6H, s), 4.99 (2H, s), 6.97 (1H, d, J = 7.6 Hz), 7.05 (1H, t, J = 7.6 Hz), 7.20 (1H, t, J = 7.6 Hz), 7.39 (1H, d, J = 7.6 Hz), 7.48 (1H, dd, J = 8.3, 1.8 Hz), 7.55 (2H, t, J = 7.6 Hz), 7.59-7.68 (2H, m), 7.75 (1H, d, J = 1.8 Hz), 8.29 (2H, d, J = 7.6 Hz), 13.32 (1H, br s). 457 455
    1-259
    Figure US20200087266A1-20200319-C00479
         		1H-NMR (DMSO-D6) δ: 0.77 (6H, t, J = 7.3 Hz), 1.03-1.19 (4H, m), 1.36-1.50 (4H, m), 3.46 (2H, s), 4.33 (2H, d, J = 6.0 Hz), 4.62 (1H, br s), 7.47 (1H, d, J = 8.4 Hz), 7.53-7.60 (3H, m), 7.61-7.69 (2H, m), 8.12 (1H, t, J = 6.0 Hz), 8.34 (2H, d, J = 7.6 Hz), 13.35 (1H, br s). 469 467
    1-260
    Figure US20200087266A1-20200319-C00480
         		1H-NMR (DMSO-D6) δ: 0.84 (6H, t, J = 7.2 Hz), 1.14-1.29 (2H, m), 1.30-1.58 (6H, m), 3.02 (2H, s), 4.39 (2H, s), 7.48 (1H, dd, J = 8.3, 2.1 Hz), 7.57 (2H, t, J = 7.9 Hz), 7.64- 7.70 (3H, m), 8.34 (2H, d, J = 7.9 Hz), 13.36 (1H, br s). 451 449
    1-261
    Figure US20200087266A1-20200319-C00481
         		1H-NMR (DMSO-D6) δ: 0.96-1.06 (3.9H, m), 1.10 (2.1H, t, J = 7.2 Hz), 2.31 (0.6H, q, J = 7.2 Hz), 2.42 (1.4H, q, J = 7.2 Hz), 3.29-3.35 (2H, m), 4.56 (1.4H, s), 4.63 (0.6H, s), 7.45 (1H, d, J = 8.3 Hz), 7.54-7.69 (5H, m), 8.34 (2H, d, J = 7.9 Hz), 13.33 (1H, br s). 397 395
    1-262
    Figure US20200087266A1-20200319-C00482
         		1H-NMR (DMSO-D6) δ: 1.28 (3H, s), 2.75 (2H, d, J = 15.7 Hz), 3.36 (2H, d, J = 15.7 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.06-7.11 (2H, m), 7.14-7.18 (2H, m), 7.44 (1H, dd, J = 8.3, 2.1 Hz), 7.52-7.62 (4H, m), 7.66 (1H, t, J = 7.4 Hz), 8.34 (2H, d, J = 7.4 Hz), 8.41 (1H, t, J = 6.0 Hz), 13.32 (1H, br s). 471 469
    1-263
    Figure US20200087266A1-20200319-C00483
         		1H-NMR (DMSO-D6) δ: 1.13 (3H, t, J = 6.9 Hz), 1.50 (6H, s), 3.42 (2H, br s), 4.66 (2H, s), 7.41 (1H, dd, J = 8.3, 1.8 Hz), 7.56 (2H, t, J = 7.9 Hz), 7.61-7.69 (3H, m), 8.34 (2H, d, J = 7.9 Hz), 13.33 (1H, br s). 479 477
  • TABLE 1-34
    1-264
    Figure US20200087266A1-20200319-C00484
         		1H-NMR (DMSO-D6) δ: 1.31 (3H, s), 2.91 (2H, d, J = 16.0 Hz), 3.04 (3H, br s), 3.51 (2H, d, J = 16.0 Hz), 4.66 (2H, br s), 7.11-7.15 (2H, m), 7.17-7.21 (2H, m), 7.45 (1H, dd, J = 8.3, 2.1 Hz), 7.54 (2H, t, J = 7.9 Hz), 7.61- 7.67 (3H, m), 8.33 (2H, d, J = 7.9 Hz), 13.33 (1H, br s). 485 483
    1-265
    Figure US20200087266A1-20200319-C00485
         		1H-NMR (DMSO-D6) δ: 1.34-1.42 (2H, m), 1.40 (9H, s), 1.53-1.63 (2H, m), 1.96 (2H, t, J = 6.8 Hz), 2.86-3.00 (2H, m), 3.25 (2H, t, J = 6.8 Hz), 3.78-3.87 (2H, m), 4.46 (2H, s), 7.42 (1H, dd, J = 8.3, 2.1 Hz), 7.54-7.59 (2H, m), 7.60-7.69 (3H, m), 8.34 (2H, d, J = 7.7 Hz), 13.34 (1H, br s). 550 548
    1-266
    Figure US20200087266A1-20200319-C00486
         		1H-NMR (DMSO-D6) δ: 0.83 (9H, s), 0.90- 0.99 (1H, m), 1.41-1.67 (6H, m), 1.96-2.03 (2H, m), 2.92 (2H, s), 4.38 (2H, s), 7.47 (1H, dd, J = 8.3, 1.8 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.63-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s). 491 489 4
    1-267
    Figure US20200087266A1-20200319-C00487
         		1H-NMR (DMSO-D6) δ: 1.48-1.65 (4H, m), 1.67-1.77 (2H, m), 1.82-1.91 (2H, m), 3.03 (2H, s), 3.21 (3H, s), 3.23-3.29 (1H, m), 4.40 (2H, s), 7.48 (1H, dd, J = 8.1, 1.8 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.63-7.70 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s). 465 463 5
  • TABLE 2-1
    MS MS
    Example Structure NMR (M + H) (M − H) Note
    2-1
    Figure US20200087266A1-20200319-C00488
         		1H-NMR (DMSO-D6) δ: 2.24 (6H, s), 7.21 (2H, d, J = 7.6 Hz), 7.37 (1H, t, J = 7.6 Hz), 7.91 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.35 (1H, br s). 346 344
    2-2
    Figure US20200087266A1-20200319-C00489
         		1H-NMR (CDCl3) δ: 2.34 (6H, s), 7.17 (2H, d, J = 7.7 Hz), 7.33 (1H, t, J = 7.7 Hz), 7.49 (2H, t, J = 7.7 Hz), 7.60 (1H, t, J = 7.7 Hz), 8.52 (2H, dd, J = 7.7, 1.2 Hz), 11.87 (1H, s). 278 276
    2-3
    Figure US20200087266A1-20200319-C00490
         		1H-NMR (CDCl3) δ: 2.32 (6H, s), 2.72 (3H, s), 7.17 (2H, d, J = 7.7 Hz), 7.34 (1H, t, J = 7.7 Hz), 7.53-7.56 (2H, m), 8.12-8.16 (1H, m), 11.29 (1H, s). 360 358
    2-4
    Figure US20200087266A1-20200319-C00491
         		1H-NMR (DMSO-D6) δ: 3.84 (3H, s), 7.25 (2H, d, J = 8.3 Hz), 7.59 (1H, t, J = 8.3 Hz), 7.92 (2H, d, J = 8.3 Hz), 8.51 (2H, d, J = 8.3 Hz), 13.59 (1H, br s). 382 380
    2-5
    Figure US20200087266A1-20200319-C00492
         		1H-NMR (DMSO-D6) δ: 2.22 (6H, s), 5.22 (2H, s), 7.13-7.21 (2H, m), 7.32-7.37 (2H, m), 7.38-7.43 (2H, m), 7.46-7.48 (2H, m), 8.28- 8.32 (2H, m), 12.98 (1H, br s). 384 382
    2-6
    Figure US20200087266A1-20200319-C00493
         		1H-NMR (DMSO-D6) δ: 2.20 (6H, s), 6.86 (2H, dt, J = 9.4, 2.4 Hz), 7.17 (2H, d, J = 7.7 Hz), 7.32 (1H, t, J = 7.6 Hz), 8.19 (2H, dt, J = 9.5, 2.3 Hz), 10.32 (1H, br s), 12.87 (1H, br s). 294 292
    2-7
    Figure US20200087266A1-20200319-C00494
         		1H-NMR (DMSO-D6) δ: 1.32 (9H, s), 2.22 (6H, s), 7.20 (2H, d, J = 7.7 Hz), 7.35 (1H, t, J = 7.7 Hz), 7.56 (2H, d, J = 8.5 Hz), 8.28 (2H, d, J = 8.5 Hz), 13.08 (1H, br s). 334 332
    2-8
    Figure US20200087266A1-20200319-C00495
         		1H-NMR (DMSO-D6) δ: 1.23 (6H, d, J = 7.3 Hz), 2.22 (6H, s), 2.90-3.05 (1H, m), 7.20 (2H, d, J = 7.7 Hz), 7.35 (1H, t, J = 7.7 Hz), 7.41 (2H, d, J = 8.3 Hz), 8.27 (2H, d, J = 8.3 Hz), 13.09 (1H, br s). 320 318
    2-9
    Figure US20200087266A1-20200319-C00496
         		1H-NMR (DMSO-D6) δ: 2.24 (6H, s), 7.19 (2H, d, J = 7.7 Hz), 7.34 (1H, t, J = 7.7 Hz), 7.73 (1H, d, J = 8.1 Hz), 7.85 (1H, d, J = 10.7 Hz), 8.24 (1H, t, J = 7.9 Hz), 13.42 (1H, br s). 364 362
  • TABLE 2-2
    2-10
    Figure US20200087266A1-20200319-C00497
         		1H-NMR (DMSO-D6) δ: 7.50 (1H, t, J = 8.8 Hz), 7.57 (1H, d, J = 8.4 Hz), 7.67-7.73 (1H, m), 7.94 (2H, d, J = 8.1 Hz), 8.51 (2H, d, J = 8.1 Hz), 13.90 (1H, br s). 370 368
    2-11
    Figure US20200087266A1-20200319-C00498
         		1H-NMR (DMSO-D6) δ: 2.24 (6H, s), 7.20 (2H, d, J = 7.7 Hz), 7.35 (1H, t, J = 7.7 Hz), 7.45-7.47 (3H, m), 7.59-7.63 (2H, m), 7.71 (2H, dd, J = 6.9, 2.0 Hz), 8.37 (2H, dd, J = 6.9, 2.0 Hz), 13.20 (1H, br s). 378 376
    2-12
    Figure US20200087266A1-20200319-C00499
         		1H-NMR (DMSO-D6) δ: 2.22 (6H, s), 2.89- 3.00 (4H, m), 7.16-7.29 (7H, m), 7.33 (1H, d, J = 7.7 Hz), 7.38 (2H, d, J = 8.4 Hz), 8.24 (2H, d, J = 8.4 Hz), 13.09 (1H, br s). 382 380
    2-13
    Figure US20200087266A1-20200319-C00500
         		1H-NMR (DMSO-D6) δ: 2.23 (6H, s), 2.39 (3H, s), 7.20 (2H, d, J = 7.6 Hz), 7.35 (1H, t, J = 7.6 Hz), 7.40-7.48 (2H, m), 8.11-8.18 (2H, m), 13.14 (1H, br s). 292 290
    2-14
    Figure US20200087266A1-20200319-C00501
         		1H-NMR (DMSO-D6) δ: 2.23 (6H, s), 3.83 (3H, s), 7.18-7.24 (3H, m), 7.36 (1H, t, J = 7.8 Hz), 7.45 (1H, t, J = 7.8 Hz), 7.83-7.86 (1H, m), 7.94 (1H, d, J = 7.8 Hz), 13.17 (1H, br s). 308 306
    2-15
    Figure US20200087266A1-20200319-C00502
         		1H-NMR (DMSO-D6) δ: 2.23 (6H, s), 7.21 (2H, d, J = 7.6 Hz), 7.36 (1H, t, J = 7.6 Hz), 7.47-7.53 (1H, m), 7.57-7.63 (1H, m), 8.00- 8.05 (1H, m), 8.19 (1H, dt, J = 7.9, 1.3 Hz), 13.27 (1H, br s). 296 294
    2-16
    Figure US20200087266A1-20200319-C00503
         		1H-NMR (DMSO-D6) δ: 6.97 (1H, dd, J = 8.1, 0.7 Hz), 7.05 (1H, dd, J = 8.1, 0.7 Hz), 7.39 (1H, t, J = 8.1 Hz), 7.90 (2H, d, J = 8.4 Hz), 8.50 (2H, d, J = 8.4 Hz), 10.74 (1H, br s), 13.38 (1H, br s). 368 366
    2-17
    Figure US20200087266A1-20200319-C00504
         		1H-NMR (DMSO-D6) δ: 1.20 (3H, t, J = 7.0 Hz), 4.12 (2H, q, J = 7.0 Hz), 7.21 (2H, d, J = 8.3 Hz), 7.55 (1H, t, J = 8.3 Hz), 7.91 (2H, d, J = 8.3 Hz), 8.50 (2H, d, J = 8.3 Hz), 13.53 (1H, br s). 396 394
    2-18
    Figure US20200087266A1-20200319-C00505
         		1H-NMR (DMSO-D6) δ: 1.31-1.40 (3H, m), 1.45-1.54 (3H, m), 1.65-1.72 (2H, m), 1.81- 1.88 (2H, m), 2.22 (6H, s), 2.67-2.71 (1H, m), 7.20 (2H, d, J = 7.7 Hz), 7.35 (1H, t, J = 7.7 Hz), 7.51 (2H, dd, J = 6.7, 1.9 Hz), 8.29 (2H, dd, J = 6.7, 1.9 Hz), 13.16 (1H, br s). 384 382
  • TABLE 2-3
    2-19
    Figure US20200087266A1-20200319-C00506
         		1H-NMR (DMSO-D6) δ: 2.29 (3H, s), 7.38- 7.41 (1H, m), 7.47-7.52 (2H, m), 7.92 (2H, d, J = 8.1 Hz), 8.52 (2H, d, J = 8.1 Hz), 13.61 (1H, br s). 366 364
    2-20
    Figure US20200087266A1-20200319-C00507
         		1H-NMR (DMSO-D6) δ: 0.80 (3H, t, J = 7.3 Hz), 1.55-1.63 (2H, m), 4.02 (2H, t, J = 6.3 Hz), 7.22 (2H, t, J = 8.3 Hz), 7.55 (1H, t, J = 8.3 Hz), 7.90 (2H, d, J = 8.3 Hz), 8.50 (2H, d, J = 8.3 Hz), 13.54 (1H, br s). 410 408
    2-21
    Figure US20200087266A1-20200319-C00508
         		1H-NMR (DMSO-D6) δ: 1.18 (6H, d, J = 6.0 Hz), 4.65-4.71 (1H, m), 7.20 (1H, d, J = 8.4 Hz), 7.25 (1H, d, J = 8.4 Hz), 7.54 (1H, t, J = 8.4 Hz), 7.91 (2H, d, J = 8.4 Hz), 8.50 (2H, d, J = 8.4 Hz), 13.49 (1H, br s). 410 408
    2-22
    Figure US20200087266A1-20200319-C00509
         		1H-NMR (DMSO-D6) δ: 3.59-3.62 (2H, br m), 4.09 (2H, t, J = 5.0 Hz), 4.81 (1H, br s), 7.24 (2H, t, J = 8.3 Hz), 7.56 (1H, t, J = 8.3 Hz), 7.92 (2H, d, J = 8.4 Hz), 8.52 (2H, d, J = 8.4 Hz), 13.49 (1H, s). 412 410
    2-23
    Figure US20200087266A1-20200319-C00510
         		1H-NMR (DMSO-D6) δ: 1.71-1.77 (2H, m), 3.39 (2H, t, J = 6.2 Hz), 4.14 (2H, t, J = 6.2 Hz), 4.45 (1H, br s), 7.23 (1H, d, J = 8.3 Hz), 7.24 (1H, d, J = 8.3 Hz), 7.56 (1H, t, J = 8.3 Hz), 7.92 (2H, d, J = 8.4 Hz), 8.52 (2H, d, J = 8.4 Hz), 13.56 (1H, br s). 426 424
    2-24
    Figure US20200087266A1-20200319-C00511
         		1H-NMR (DMSO-D6) δ: 2.23 (6H, s), 7.20 (2H, d, J = 7.7 Hz), 7.35 (1H, t, J = 7.7 Hz), 7.60 (2H, d, J = 8.6 Hz), 8.34 (2H, d, J = 8.6 Hz), 13.21 (1H, br s). 296 294
    2-25
    Figure US20200087266A1-20200319-C00512
         		1H-NMR (DMSO-D6) δ: 2.23 (6H, s), 7.20 (2H, d, J = 7.6 Hz), 7.35 (1H, t, J = 7.6 Hz), 7.60 (2H, d, J = 8.6 Hz), 8.34 (2H, d, J = 8.6 Hz), 13.21 (1H, br s). 312 310
    2-26
    Figure US20200087266A1-20200319-C00513
         		1H-NMR (DMSO-D6) δ: 2.22 (6H, s), 2.40 (3H, s), 7.20 (2H, d, J = 7.5 Hz), 7.32-7.37 (3H, m), 8.23 (2H, d, J = 8.2 Hz), 13.08 (1H, br s). 292 290
    2-27
    Figure US20200087266A1-20200319-C00514
         		1H-NMR (DMSO-D6) δ: 2.22 (6H, s), 3.85 (3H, s), 7.07 (2H, d, J = 9.0 Hz), 7.19 (2H, d, J = 7.6 Hz), 7.34 (1H, t, J = 7.6 Hz), 8.31 (2H, d, J = 9.0 Hz), 12.98 (1H, s). 308 306
  • TABLE 2-4
    2-28
    Figure US20200087266A1-20200319-C00515
         		1H-NMR (DMSO-D6) δ: 2.24 (6H, s), 7.21 (2H, d, J = 7.7 Hz), 7.37 (1H, t, J = 7.7 Hz), 7.80 (1H, t, J = 7.9 Hz), 8.03 (1H, d, J = 7.9 Hz), 8.57 (1H, s), 8.62 (1H, d, J = 7.9 Hz), 13.33 (1H, br s). 346 344
    2-29
    Figure US20200087266A1-20200319-C00516
         		1H-NMR (DMSO-D6) δ: 2.22 (6H, s), 5.19 (2H, s), 7.20 (2H, d, J = 7.5 Hz), 7.27-7.42 (5H, m), 7.43-7.49 (3H, m), 7.92-7.96 (2H, m), 13.17 (1H, br s). 384 382
    2-30
    Figure US20200087266A1-20200319-C00517
         		1H-NMR (DMSO-D6) δ: 2.22 (6H, s), 6.99- 7.03 (1H, m), 7.20 (2H, d, J = 7.7 Hz), 7.30- 7.37 (2H, m), 7.76-7.80 (2H, m), 9.69 (1H, s), 13.12 (1H, brs). 294 292
    2-31
    Figure US20200087266A1-20200319-C00518
         		1H-NMR (DMSO-D6) δ: 0.76 (3H, t, J = 7.4 Hz), 1.22-1.31 (2H, m), 1.53-1.60 (2H, m), 4.07 (2H, t, J = 6.2 Hz), 7.23 (2H, dd, J = 8.4, 2.2 Hz), 7.56 (1H, t, J = 8.4 Hz), 7.92 (2H, d, J = 8.4 Hz), 8.51 (2H, d, J = 8.4 Hz), 13.56 (1H, br s). 424 422
    2-32
    Figure US20200087266A1-20200319-C00519
         		1H-NMR (DMSO-D6) δ: 5.24 (2H, s), 7.35- 7.24 (7H, m), 7.57 (1H, t, J = 8.3 Hz), 7.93 (2H, d, J = 8.2 Hz), 8.52 (2H, d, J = 8.2 Hz), 13.66 (1H, br s). 458 456
    2-33
    Figure US20200087266A1-20200319-C00520
         		1H-NMR (DMSO-D6) δ: 0.80 (6H, d, J = 6.7 Hz), 1.82-1.92 (1H, m), 3.83 (2H, d, J = 6.0 Hz), 7.20 (2H, dd, J = 8.3, 3.5 Hz), 7.54 (1H, t, J = 8.3 Hz), 7.90 (2H, d, J = 8.4 Hz), 8.50 (2H, d, J = 8.4 Hz), 13.56 (1H, br s). 424 422
    2-34
    Figure US20200087266A1-20200319-C00521
         		1H-NMR (DMSO-D6) δ: 3.12 (3H, s), 3.53 (2H, t, J = 4.5 Hz), 4.18 (2H, t, J = 4.5 Hz), 7.24 (2H, dd, J = 8.3, 3.8 Hz), 7.55 (1H, t, J = 8.3 Hz), 7.90 (2H, d, J = 8.4 Hz), 8.50 (2H, d, J = 8.4 Hz), 13.54 (1H, br s). 426 424
    2-35
    Figure US20200087266A1-20200319-C00522
         		1H-NMR (DMSO-D6) δ: 3.83 (3H, s), 5.22 (2H, s), 7.16 (2H, d, J = 8.8 Hz), 7.22 (2H, d, J = 8.8 Hz), 7.37-7.32 (1H, m), 7.43-7.38 (2H, m), 7.50-7.45 (2H, m), 7.61-7.53 (1H, m), 8.28 (2H, d, J = 8.8 Hz), 13.17 (1H, s). 420 418
    2-36
    Figure US20200087266A1-20200319-C00523
         		1H-NMR (DMSO-D6) δ: 4.90 (2H, q, J = 8.7 Hz), 7.38 (2H, dd, J = 8.4, 2.4 Hz), 7.64 (1H, t, J = 8.4 Hz), 7.93 (2H, d, J = 8.2 Hz), 8.51 (2H, d, J = 8.2 Hz), 13.68 (1H, br s). 450 448
  • TABLE 2-5
    2-37
    Figure US20200087266A1-20200319-C00524
         		1H-NMR (DMSO-D6) δ: 0.99-1.10 (2H, m), 1.13-1.30 (3H, m), 1.61-1.83 (6H, m), 2.20 (6H, s), 3.86 (2H, d, J = 6.3 Hz), 7.04 (2H, d, J = 9.1 Hz), 7.17 (2H, d, J = 7.7 Hz), 7.32 (1H, t, J = 7.7 Hz), 8.26 (2H, d, J = 9.1 Hz), 12.95 (1H, br s). 390 388
    2-38
    Figure US20200087266A1-20200319-C00525
         		1H-NMR (DMSO-D6) δ: 1.28 (6H, d, J = 6.0 Hz), 2.20 (6H, s), 4.70-4.76 (1H, m), 7.02 (2H, d, J = 8.8 Hz), 7.18 (2H, d, J = 7.6 Hz), 7.32 (1H, t, J = 7.6 Hz), 8.26 (2H, d, J = 8.8 Hz), 12.94 (1H, br s). 336 334
    2-39
    Figure US20200087266A1-20200319-C00526
         		1H-NMR (DMSO-D6) δ: 0.88 (6H, d, J = 6.4 Hz), 1.84-1.94 (1H, m), 2.23 (6H, s), 2.54 (2H, d, J = 6.9 Hz), 7.20 (2H, d, J = 7.7 Hz), 7.30-7.38 (3H, m), 8.26 (2H, d, J = 8.5 Hz), 13.09 (1H, br s). 334 332
    2-40
    Figure US20200087266A1-20200319-C00527
         		1H-NMR (DMSO-D6) δ: 1.35 (3H, t, J = 6.9 Hz), 2.22 (6H, s), 4.13 (2H, q, J = 6.9 Hz), 7.05 (2H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.5 Hz), 7.34 (1H, t, J = 7.5 Hz), 8.29 (2H, d, J = 8.8 Hz), 12.97 (1H, br s). 322 320
    2-41
    Figure US20200087266A1-20200319-C00528
         		1H-NMR (DMSO-D6) δ: 0.99 (3H, t, J = 7.4 Hz), 1.71-1.80 (2H, m), 2.22 (6H, s), 4.03 (2H, t, J = 6.6 Hz), 7.06 (2H, d, J = 9.0 Hz), 7.20 (2H, d, J = 7.5 Hz), 7.34 (1H, t, J = 7.5 Hz), 8.29 (2H, d, J = 9.0 Hz), 12.98 (1H, br s). 336 334
    2-42
    Figure US20200087266A1-20200319-C00529
         		1H-NMR (DMSO-D6) δ: 0.99 (6H, d, J = 6.8 Hz), 2.01-2.08 (1H, m), 2.22 (6H, s), 3.85 (2H, d, J = 6.6 Hz), 7.06 (2H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.5 Hz), 7.35 (1H, t, J = 7.5 Hz), 8.29 (2H, d, J = 8.8 Hz), 12.97 (1H, br s). 350 348
    2-43
    Figure US20200087266A1-20200319-C00530
         		1H-NMR (DMSO-D6) δ: 1.58 (3H, d, J = 6.4 Hz), 2.19 (6H, s), 5.66 (1H, q, J = 6.4 Hz), 7.04 (2H, d, J = 9.0 Hz), 7.17 (2H, d, J = 7.7 Hz), 7.23-7.28 (1H, m), 7.30-7.37 (3H, m), 7.41-7.43 (2H, m), 8.20 (2H, d, J = 9.0 Hz), 12.96 (1H, br s). 398 396
    2-44
    Figure US20200087266A1-20200319-C00531
         		1H-NMR (DMSO-D6) δ: 1.58 (3H, d, J = 6.4 Hz), 2.19 (6H, s), 5.66 (1H, q, J = 6.4 Hz), 7.04 (2H, d, J = 9.0 Hz), 7.18 (2H, d, J = 7.7 Hz), 7.24-7.28 (1H, m), 7.30-7.37 (3H, m), 7.41-7.43 (2H, m), 8.20 (2H, d, J = 9.0 Hz), 12.96 (1H, br s). 398 396
    2-45
    Figure US20200087266A1-20200319-C00532
         		1H-NMR (DMSO-D6) δ: 5.32 (2H, s), 7.34- 7.27 (4H, m), 7.57 (1H, t, J = 8.2 Hz), 7.71 (1H, td, J = 7.7, 1.7 Hz), 7.93 (2H, d, J = 8.2 Hz), 8.55-8.51 (3H, m), 13.74 (1H, s). 459 457
  • TABLE 2-6
    2-46
    Figure US20200087266A1-20200319-C00533
         		1H-NMR (DMSO-D6) δ: 1.23-1.32 (1H, m), 1.35-1.57 (5H, m), 1.68-1.75 (2H, m), 1.92- 1.98 (2H, m), 2.22 (6H, s), 4.47-4.51 (1H, m), 7.06 (2H, d, J = 8.8 Hz), 7.19 (2H, d, J = 7.6 Hz), 7.34 (1H, t, J = 7.6 Hz), 8.27 (2H, d, J = 8.8 Hz), 12.96 (1H, br s). 376 374
    2-47
    Figure US20200087266A1-20200319-C00534
         		1H-NMR (DMSO-D6) δ: 0.94 (6H, d, J = 4.2 Hz), 1.26-1.35 (2H, m), 1.41-1.48 (2H, m), 1.56-1.65 (2H, m), 1.81-1.87 (2H, m), 2.22 (6H, s), 4.46-4.50 (1H, m), 7.06 (2H, d, J = 8.8 Hz), 7.19 (2H, d, J = 7.5 Hz), 7.34 (1H, t, J = 7.5 Hz), 8.27 (2H, d, J = 8.8 Hz), 12.96 (1H, br s). 404 402
    2-48
    Figure US20200087266A1-20200319-C00535
         		1H-NMR (DMSO-D6) δ: 2.23 (6H, s), 5.31 (2H, s), 7.19-7.23 (2H, m), 7.32-7.43 (4H, m), 7.48-7.53 (2H, m), 7.63 (1H, d, J = 8.4 Hz), 7.97 (1H, dd, J = 8.4, 1.7 Hz), 8.10 (1H, d, J = 1.7 Hz), 13.22 (1H, br s). 418 416
    2-49
    Figure US20200087266A1-20200319-C00536
         		1H-NMR (DMSO-D6) δ: 0.99 (3H, t, J = 7.5 Hz), 2.55 (2H, q, J = 7.5 Hz), 5.22 (2H, s), 7.07 (1H, t, J = 6.8 Hz), 7.16 (1H, d, J = 6.8 Hz), 7.22 (1H, t, J = 6.8 Hz), 7.27 (1H, d, J = 8.2 Hz), 7.31 (1H, d, J = 6.8 Hz), 7.42 (1H, d, J = 8.2 Hz), 7.60 (1H, t, J = 8.2 Hz), 7.91 (2H, d, J = 8.6 Hz), 8.48 (2H, d, J = 8.6 Hz), 13.63 (1H, s). 486 484
    2-50
    Figure US20200087266A1-20200319-C00537
         		1H-NMR (DMSO-D6) δ: 1.30-1.38 (2H, m), 1.50-1.65 (4H, m), 1.74-1.82 (2H, m), 2.22 (6H, s), 2.29-2.36 (1H, m), 3.95 (2H, d, J = 7.1 Hz), 7.06 (2H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.7 Hz), 7.34 (1H, t, J = 7.7 Hz), 8.28 (2H, d, J = 8.8 Hz), 12.97 (1H, br s). 376 374
    2-51
    Figure US20200087266A1-20200319-C00538
         		1H-NMR (DMSO-D6) δ: 1.81-1.94 (4H, m), 2.05-2.12 (2H, m), 2.22 (6H, s), 2.70-2.78 (1H, m), 4.05 (2H, d, J = 6.6 Hz), 7.06 (2H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.5 Hz), 7.34 (1H, t, J = 7.5 Hz), 8.29 (2H, d, J = 8.8 Hz), 12.97 (1H, br s). 362 360
    2-52
    Figure US20200087266A1-20200319-C00539
         		1H-NMR (DMSO-D6) δ: 1.38 (3H, t, J = 6.9 Hz), 2.22 (6H, s), 4.19 (2H, q, J = 6.9 Hz), 7.19 (2H, d, J = 7.6 Hz), 7.34 (1H, t, J = 7.6 Hz), 7.58 (1H, d, J = 8.3 Hz), 7.92 (1H, dd, J = 8.3, 1.7 Hz), 7.96 (1H, d, J = 1.7 Hz), 13.19 (1H, br s). 356 354
    2-53
    Figure US20200087266A1-20200319-C00540
         		1H-NMR (DMSO-D6) δ: 2.18 (3H, s), 5.18 (2H, s), 7.04 (1H, t, J = 7.4 Hz), 7.16-7.09 (2H, m), 7.21 (1H, d, J = 7.9 Hz), 7.28 (1H, d, J = 7.4 Hz), 7.34 (1H, d, J = 8.4 Hz), 7.57- 7.49 (1H, m), 7.88 (2H, d, J = 8.4 Hz), 8.47 (2H, d, J = 8.4 Hz), 13.63 (1H, s). 472 470
    2-54
    Figure US20200087266A1-20200319-C00541
         		1H-NMR (DMSO-D6) δ: 1.20 (3H, t, J = 7.5 Hz), 2.23 (6H, s), 2.80 (2H, q, J = 7.5 Hz), 7.21 (2H, d, J = 7.6 Hz), 7.36 (1H, t, J = 7.6 Hz), 7.58 (1H, d, J = 8.4 Hz), 8.17 (1H, dd, J = 8.4, 2.0 Hz), 8.29 (1H, d, J = 2.0 Hz), 13.20 (1H, br s). 340 338
  • TABLE 2-7
    2-55
    Figure US20200087266A1-20200319-C00542
         		1H-NMR (DMSO-D6) δ: 2.11 (3H, s), 5.17 (2H, s), 7.04 (1H, d, J = 7.7 Hz), 7.17-7.06 (3H, m), 7.25 (1H, d, J = 8.3 Hz), 7.29 (1H, d, J = 8.3 Hz), 7.57 (1H, t, J = 8.3 Hz), 7.91 (2H, d, J = 8.1 Hz), 8.52 (2H, d, J = 8.1 Hz), 13.64 (1H, br s). 472 470
    2-56
    Figure US20200087266A1-20200319-C00543
         		1H-NMR (DMSO-D6) δ: 2.22 (3H, s), 5.16 (2H, s), 7.06 (2H, d, J = 7.9 Hz), 7.20 (2H, d, J = 7.9 Hz), 7.23 (1H, d, J = 7.9 Hz), 7.28 (1H, d, J = 7.9 Hz), 7.55 (1H, t, J = 8.4 Hz), 7.92 (2H, d, J = 8.4 Hz), 8.49 (2H, d, J = 8.4 Hz), 13.62 (1H, br s). 472 470
    2-57
    Figure US20200087266A1-20200319-C00544
         		1H-NMR (DMSO-D6) δ: 1.34-1.23 (2H, m), 1.62-1.38 (6H, m), 1.72-1.63 (2H, m), 1.86- 1.77 (2H, m), 2.00-1.88 (1H, m), 2.22 (6H, s), 3.86 (2H, d, J = 6.8 Hz), 7.06 (2H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.6 Hz), 7.34 (1H, t, J = 7.6 Hz), 8.28 (2H, d, J = 8.8 Hz), 12.97 (1H, br s). 404 402
    2-58
    Figure US20200087266A1-20200319-C00545
         		1H-NMR (DMSO-D6) δ: 1.01 (9H, s), 2.22 (6H, s), 3.74 (2H, s), 7.07 (2H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.7 Hz), 7.35 (1H, t, J = 7.7 Hz), 8.29 (2H, d, J = 8.8 Hz), 12.97 (1H, br s). 364 362
    2-59
    Figure US20200087266A1-20200319-C00546
         		1H-NMR (DMSO-D6) δ: 2.24 (6H, s), 5.46 (2H, s), 7.02 (1H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.7 Hz), 7.31-7.41 (4H, m), 7.46-7.49 (2H, m), 8.53 (1H, dd, J = 8.8, 2.0 Hz), 9.10 (1H, d, J = 2.0 Hz), 13.13 (1H, br s). 385 383
    2-60
    Figure US20200087266A1-20200319-C00547
         		1H-NMR (DMSO-D6) δ: 5.30 (2H, s), 7.28 (1H, d, J = 7.9 Hz), 7.30-7.35 (2H, m), 7.59 (1H, t, J = 8.4 Hz), 7.73 (1H, dt, J = 7.9, 1.5 Hz), 7.93 (2H, d, J = 8.4 Hz), 8.48 (1H, dd, J = 4.9, 1.5 Hz), 8.51 (2H, d, J = 8.4 Hz), 8.56 (1H, d, J = 1.5 Hz), 13.67 (1H, br s). 459 457
    2-61
    Figure US20200087266A1-20200319-C00548
         		1H-NMR (DMSO-D6) δ: 5.33 (2H, s), 7.23- 7.32 (4H, m), 7.57 (1H, t, J = 8.4 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.48 (2H, d, J = 5.3 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.72 (1H, br s). 459 457
    2-62
    Figure US20200087266A1-20200319-C00549
         		1H-NMR (DMSO-D6) δ: 1.00 (9H, s), 3.73 (2H, s), 5.30 (2H, s), 7.08 (2H, t, J = 4.5 Hz), 7.30-7.20 (3H, m), 7.33 (1H, d, J = 7.7 Hz), 7.52 (1H, t, J = 8.1 Hz), 7.69 (1H, td, J = 7.7, 1.7 Hz), 8.28 (2H, d, J = 8.8 Hz), 8.53-8.49 (1H, m), 13.27 (1H, br s). 477 475
    2-63
    Figure US20200087266A1-20200319-C00550
         		1H-NMR (DMSO-D6) δ: 1.01 (9H, s), 1.46 (3H, d, J = 6.2 Hz), 3.74 (2H, s), 5.60 (1H, q, J = 6.2 Hz), 7.04-7.11 (3H, m), 7.17 (1H, d, J = 7.7 Hz), 7.27 (1H, dd, J = 7.0, 5.1 Hz), 7.37- 7.44 (2H, m), 7.72 (1H, t, J = 7.0 Hz), 8.30 (2H, d, J = 8.8 Hz), 8.51 (1H, d, J = 4.2 Hz), 13.32 (1H, br s). 491 489
  • TABLE 2-8
    2-64
    Figure US20200087266A1-20200319-C00551
         		1H-NMR (DMSO-D6) δ: 1.01 (9H, s), 1.47 (3H, d, J = 6.3 Hz), 3.74 (2H, s), 5.60 (1H, q, J = 6.3 Hz), 7.13-7.02 (3H, m), 7.18 (1H, d, J = 7.7 Hz), 7.27 (1H, dd, J = 7.1, 5.2 Hz), 7.48- 7.36 (2H, m), 7.72 (1H, t, J = 7.1 Hz), 8.31 (2H, d, J = 8.8 Hz), 8.51 (1H, d, J = 4.2 Hz), 13.32 (1H, br s). 491 489
    2-65
    Figure US20200087266A1-20200319-C00552
         		1H-NMR (DMSO-D6) δ: 1.01 (9H, s), 1.40 (3H, d, J = 6.2 Hz), 3.74 (2H, s), 5.62 (1H, q, J = 6.2 Hz), 7.05-7.15 (4H, m), 7.21-7.41 (6H, m), 8.31 (2H, d, J = 8.8 Hz), 13.21 (1H, br s). 490 488
    2-66
    Figure US20200087266A1-20200319-C00553
         		1H-NMR (DMSO-D6) δ: 0.99 (6H, d, J = 6.6 Hz), 2.04 (1H, td, J = 13.3, 6.6 Hz), 3.86 (2H, d, J = 6.6 Hz), 5.31 (2H, s), 7.08 (2H, d, J = 8.8 Hz), 7.31-7.21 (3H, m), 7.34 (1H, d, J = 7.7 Hz), 7.57-7.48 (1H, m), 7.70 (1H, td, J = 7.7, 1.6 Hz), 8.30 (2H, d, J = 8.8 Hz), 8.53 (1H, d, J = 4.9 Hz), 13.29 (1H, br s). 463 461
    2-67
    Figure US20200087266A1-20200319-C00554
         		1H-NMR (DMSO-D6) δ: 1.00 (6H, d, J = 6.6 Hz), 1.48 (3H, d, J = 6.6 Hz), 2.09-2.02 (1H, m), 3.87 (2H, d, J = 6.6 Hz), 5.61 (1H, q, J = 6.2 Hz), 7.14-7.04 (3H, m), 7.19 (1H, d, J = 7.9 Hz), 7.31-7.25 (1H, m), 7.48-7.37 (2H, m), 7.73 (1H, td, J = 7.9, 1.6 Hz), 8.32 (2H, d, J = 9.0 Hz), 8.53 (1H, d, J = 4.9 Hz), 13.33 (1H, br s). 477 475
    2-68
    Figure US20200087266A1-20200319-C00555
         		1H-NMR (DMSO-D6) δ: 1.02 (9H, s), 3.74 (2H, s), 5.40 (2H, s), 7.08 (2H, d, J = 8.8 Hz), 7.24 (2H, d, J = 7.7 Hz), 7.44 (1H, t, J = 4.9 Hz), 7.51 (1H, br s), 8.27 (2H, d, J = 8.8 Hz), 8.78 (2H, d, J = 4.9 Hz), 13.19 (1H, br s). 478 476
    2-69
    Figure US20200087266A1-20200319-C00556
         		1H-NMR (DMSO-D6) δ: 0.88 (3H, t, J = 7.2 Hz), 1.31-1.42 (4H, m), 1.69-1.76 (2H, m), 2.21 (6H, s), 4.05 (2H, t, J = 6.5 Hz), 7.04 (2H, d, J = 8.8 Hz), 7.18 (2H, d, J = 7.4 Hz), 7.33 (1H, t, J = 7.4 Hz), 8.27 (2H, d, J = 8.8 Hz), 12.96 (1H, br s). 364 362
    2-70
    Figure US20200087266A1-20200319-C00557
         		1H-NMR (DMSO-D6) δ: 0.92 (3H, t, J = 7.3 Hz), 1.38-1.48 (2H, m), 1.67-1.74 (2H, m), 2.21 (6H, s), 4.06 (2H, t, J = 6.5 Hz), 7.05 (2H, d, J = 8.8 Hz), 7.18 (2H, d, J = 7.7 Hz), 7.33 (1H, t, J = 7.7 Hz), 8.27 (2H, d, J = 8.8 Hz), 12.96 (1H, br s). 350 348
    2-71
    Figure US20200087266A1-20200319-C00558
         		1H-NMR (DMSO-D6) δ: 0.92 (6H, d, J = 6.6 Hz), 1.63 (2H, q, J = 6.6 Hz), 1.72-1.82 (1H, m), 2.21 (6H, s), 4.08 (2H, t, J = 6.6 Hz), 7.05 (2H, d, J = 8.8 Hz), 7.18 (2H, d, J = 7.4 Hz), 7.33 (1H, t, J = 7.4 Hz), 8.27 (2H, d, J = 8.8 Hz), 12.96 (1H, br s). 364 362
    2-72
    Figure US20200087266A1-20200319-C00559
         		1H-NMR (DMSO-D6) δ: 0.98 (6H, d, J = 6.5 Hz), 2.00-2.07 (1H, m), 2.19 (3H, s), 3.84 (2H, d, J = 6.5 Hz), 5.31 (2H, s), 7.05 (2H, d, J = 8.8 Hz), 7.20-7.26 (2H, m), 7.40 (1H, d, J = 8.1 Hz), 7.50-7.57 (2H, m), 8.23 (2H, d, J = 8.8 Hz), 8.33 (1H, d, J = 3.7 Hz), 13.25 (1H, br s). 477 475
  • TABLE 2-9
    2-73
    Figure US20200087266A1-20200319-C00560
         		1H-NMR (DMSO-D6) δ: 0.90 (6H, t, J = 7.4 Hz), 1.60-1.69 (4H, m), 2.22 (6H, s), 4.36- 4.42 (1H, m), 7.06 (2H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.5 Hz), 7.35 (1H, t, J = 7.5 Hz), 8.28 (2H, d, J = 8.8 Hz), 12.97 (1H, br s). 364 362
    2-74
    Figure US20200087266A1-20200319-C00561
         		1H-NMR (DMSO-D6) δ: 0.31-0.35 (2H, m), 0.55-0.59 (2H, m), 1.20-1.27 (1H, m), 2.21 (6H, s), 3.91 (2H, d, J = 7.0 Hz), 7.04 (2H, d, J = 8.8 Hz), 7.18 (2H, d, J = 7.4 Hz), 7.33 (1H, t, J = 7.4 Hz), 8.27 (2H, d, J = 8.8 Hz), 12.95 (1H, br s). 348 346
    2-75
    Figure US20200087266A1-20200319-C00562
         		1H-NMR (DMSO-D6) δ: 1.94-1.80 (4H, m), 2.11-2.03 (2H, m), 2.76-2.69 (1H, m), 4.05 (2H, d, J = 6.7 Hz), 5.29 (2H, s), 7.07 (2H, d, J = 8.8 Hz), 7.29-7.23 (3H, m), 7.32 (1H, d, J = 7.9 Hz), 7.56-7.48 (1H, m), 7.71-7.66 (1H, m), 8.28 (2H, d, J = 8.8 Hz), 8.52-8.50 (1H, m), 13.28 (1H, br s). 475 473
    2-76
    Figure US20200087266A1-20200319-C00563
         		1H-NMR (DMSO-D6) δ: 1.40-1.31 (3H, m), 1.54-1.45 (3H, m), 1.73-1.65 (2H, m), 1.88- 1.80 (2H, m), 2.73-2.66 (1H, m), 5.31 (2H, s), 7.31-7.25 (3H, m), 7.33 (1H, d, J = 7.7 Hz), 7.57-7.51 (3H, m), 7.70 (1H, td, J = 7.7, 1.7 Hz), 8.30 (2H, d, J = 8.4 Hz), 8.52 (1H, d, J = 4.6 Hz), 13.53 (1H, br s). 497 495
    2-77
    Figure US20200087266A1-20200319-C00564
         		1H-NMR (DMSO-D6) δ: 1.30-0.99 (5H, m), 1.83-1.60 (6H, m), 3.87 (2H, d, J = 6.3 Hz), 5.29 (2H, s), 7.06 (2H, d, J = 8.8 Hz), 7.29- 7.23 (3H, m), 7.32 (1H, d, J = 7.7 Hz), 7.55- 7.49 (1H, m), 7.68 (1H, t, J = 7.7 Hz), 8.27 (2H, d, J = 8.8 Hz), 8.51 (1H, d, J = 4.9 Hz), 13.27 (1H, br s). 503 501
    2-78
    Figure US20200087266A1-20200319-C00565
         		1H-NMR (DMSO-D6) δ: 0.90 (3H, t, J = 7.3 Hz), 1.27 (3H, d, J = 6.0 Hz), 1.47-1.31 (2H, m), 1.60-1.51 (1H, m), 1.72-1.63 (1H, m), 4.65-4.59 (1H, m), 5.31 (2H, s), 7.06 (2H, d, J = 9.0 Hz), 7.31-7.24 (3H, m), 7.34 (1H, d, J = 7.7 Hz), 7.53 (1H, t, J = 7.7 Hz), 7.70 (1H, td, J = 7.7, 1.5 Hz), 8.28 (2H, d, J = 9.0 Hz), 8.53 (1H, d, J = 4.2 Hz), 13.28 (1H, br s). 477 475
    2-79
    Figure US20200087266A1-20200319-C00566
         		1H-NMR (DMSO-D6) δ: 0.90 (3H, t, J = 7.3 Hz), 1.27 (3H, d, J = 6.0 Hz), 1.47-1.33 (2H, m), 1.61-1.51 (1H, m), 1.72-1.62 (1H, m), 4.65-4.59 (1H, m), 5.31 (2H, s), 7.06 (2H, d, J = 9.0 Hz), 7.31-7.24 (3H, m), 7.34 (1H, d, J = 7.9 Hz), 7.53 (1H, t, J = 7.9 Hz), 7.72-7.68 (1H, m), 8.28 (2H, d, J = 9.0 Hz), 8.53 (1H, d, J = 4.0 Hz), 13.28 (1H, br s). 477 475
    2-80
    Figure US20200087266A1-20200319-C00567
         		1H-NMR (DMSO-D6) δ: 5.31 (2H, s), 7.30- 7.25 (3H, m), 7.33 (1H, d, J = 7.7 Hz), 7.47- 7.43 (3H, m), 7.54 (1H, t, J = 8.5 Hz), 7.62- 7.57 (2H, m), 7.73-7.67 (3H, m), 8.36 (2H, d, J = 8.4 Hz), 8.52 (1H, d, J = 4.0 Hz), 13.56 (1H, br s). 491 489
  • TABLE 2-10
    2-81
    Figure US20200087266A1-20200319-C00568
         		1H-NMR (DMSO-D6) δ: 1.76-1.54 (6H, m), 2.04-1.95 (2H, m), 2.95-2.88 (1H, m), 5.31 (2H, s), 7.34-7.25 (4H, m), 7.58-7.51 (3H, m), 7.70 (1H, td, J = 7.7, 1.8 Hz), 8.29 (2H, d, J = 8.4 Hz), 8.54-8.52 (1H, m), 13.53 (1H, br s). 483 481
    2-82
    Figure US20200087266A1-20200319-C00569
         		1H-NMR (DMSO-D6) δ: 0.81-0.76 (2H, m), 0.96-0.90 (2H, m), 1.64-1.55 (1H, m), 5.31 (2H, s), 7.33-7.25 (4H, m), 7.58-7.50 (3H, m), 7.70 (1H, td, J = 7.7, 1.7 Hz), 8.28 (2H, d, J = 8.4 Hz), 8.53 (1H, d, J = 4.0 Hz), 13.52 (1H, br s). 455 453
    2-83
    Figure US20200087266A1-20200319-C00570
         		1H-NMR (DMSO-D6) δ: 1.31-1.40 (3H, m), 1.45-1.54 (3H, m), 1.65-1.72 (2H, m), 1.81- 1.87 (2H, m), 2.67-2.72 (1H, m), 5.44 (2H, s), 7.25-7.30 (2H, m), 7.51-7.56 (4H, m), 8.14 (1H, d, J = 8.2 Hz), 8.29 (2H, d, J = 8.6 Hz), 8.94 (1H, s), 13.52 (1H, br s). 565 563
    2-84
    Figure US20200087266A1-20200319-C00571
         		1H-NMR (DMSO-D6) δ: 0.94 (6H, d, J = 4.2 Hz), 1.35-1.25 (2H, m), 1.49-1.40 (2H, m), 1.66-1.55 (2H, m), 1.89-1.79 (2H, m), 4.53- 4.44 (1H, m), 5.30 (2H, s), 7.07 (2H, d, J = 8.8 Hz), 7.31-7.21 (3H, m), 7.34 (1H, d, J = 7.7 Hz), 7.56-7.48 (1H, m), 7.70 (1H, t, J = 7.1 Hz), 8.27 (2H, d, J = 8.8 Hz), 8.53 (1H, d, J = 4.6 Hz), 13.28 (1H, s). 517 515
    2-85
    Figure US20200087266A1-20200319-C00572
         		1H-NMR (DMSO-D6) δ: 5.38 (2H, s), 7.28 (1H, d, J = 8.1 Hz), 7.31 (1H, d, J = 8.1 Hz), 7.47-7.40 (5H, m), 7.55 (1H, d, J = 8.4 Hz), 7.61-7.57 (2H, m), 7.72 (2H, d, J = 8.8 Hz), 7.88-7.84 (1H, m), 8.36 (2H, d, J = 8.8 Hz), 8.58 (1H, d, J = 4.4 Hz). 491 489
    2-86
    Figure US20200087266A1-20200319-C00573
         		1H-NMR (DMSO-D6) δ: 1.51 (3H, d, J = 6.4 Hz), 5.65 (1H, q, J = 6.4 Hz), 7.11 (1H, d, J = 8.6 Hz), 7.22 (1H, d, J = 7.7 Hz), 7.32 (1H, dd, J = 6.9, 5.4 Hz), 7.50-7.41 (5H, m), 7.63- 7.59 (2H, m), 7.81-7.73 (3H, m), 8.40 (2H, d, J = 8.6 Hz), 8.55 (1H, d, J = 4.2 Hz). 505 503
    2-87
    Figure US20200087266A1-20200319-C00574
         		1H-NMR (DMSO-D6) δ: 2.53 (3H, s), 5.40 (2H, s), 7.30 (1H, d, J = 8.2 Hz), 7.33 (1H, d, J = 8.2 Hz), 7.42-7.35 (2H, m), 7.49-7.44 (3H, m), 7.64-7.56 (3H, m), 7.73 (2H, d, J = 8.8 Hz), 7.91-7.88 (1H, m), 8.37 (2H, d, J = 8.8 Hz). 505 503
    2-88
    Figure US20200087266A1-20200319-C00575
         		1H-NMR (CDCl3) δ: 5.50 (0.90H, s), 5.60 (1.10H, s), 7.43-7.30 (5.45H, m), 7.69-7.54 (5.00H, m), 7.90-7.78 (2.00H, m), 8.05 (0.45H, d, J = 7.9 Hz), 8.71-8.60 (2.55H, m), 8.88 (0.55H, d, J = 4.2 Hz), 11.44 (0.45H, s), 14.69 (0.55H, br s). 482 480
    2-89
    Figure US20200087266A1-20200319-C00576
         		1H-NMR (DMSO-D6) δ: 0.38-0.32 (2H, m), 0.62-0.56 (2H, m), 1.30-1.20 (1H, m), 3.93 (2H, d, J = 6.8 Hz), 5.32 (2H, s), 7.08 (2H, d, J = 7.5 Hz), 7.36-7.24 (4H, m), 7.54 (1H, t, J = 8.4 Hz), 7.71 (1H, t, J = 7.6 Hz), 8.29 (2H, d, J = 7.5 Hz), 8.53 (1H, d, J = 4.6 Hz). 461 459
  • TABLE 2-11
    2-90
    Figure US20200087266A1-20200319-C00577
         		1H-NMR (DMSO-D6) δ: 0.32-0.35 (2H, m), 0.55-0.59 (2H, m), 1.20-1.25 (1H, m), 2.79 (3H, s), 2.91 (3H, s), 3.91 (2H, d, J = 7.0 Hz), 4.98 (2H, s), 7.05 (2H, d, J = 8.8 Hz), 7.16 (1H, d, J = 8.4 Hz), 7.23 (1H, d, J = 8.4 Hz), 7.51 (1H, t, J = 8.4 Hz), 8.26 (2H, d, J = 8.8 Hz), 13.15 (1H, br s). 455 453
    2-91
    Figure US20200087266A1-20200319-C00578
         		1H-NMR (DMSO-D6) δ: 1.76-1.53 (6H, m), 2.05-1.93 (2H, m), 2.95-2.88 (1H, m), 5.36 (2H, s), 7.28 (1H, dd, J = 7.9, 0.7 Hz), 7.30 (1H, d, J = 7.9 Hz), 7.43-7.36 (2H, m), 7.59- 7.50 (3H, m), 7.81 (1H, td, J = 7.7, 1.8 Hz), 8.29 (2H, d, J = 8.6 Hz), 8.57 (1H, dq, J = 5.0, 0.8 Hz). 483 481
    2-92
    Figure US20200087266A1-20200319-C00579
         		1H-NMR (DMSO-D6) δ: 1.41-1.30 (3H, m), 1.56-1.45 (3H, m), 1.74-1.64 (2H, m), 1.89- 1.80 (2H, m), 2.74-2.65 (1H, m), 5.37 (2H, s), 7.28 (1H, dd, J = 8.0, 0.6 Hz), 7.31 (1H, d, J = 8.0 Hz), 7.46-7.40 (2H, m), 7.59-7.52 (3H, m), 7.85 (1H, td, J = 7.8, 1.6 Hz), 8.29 (2H, d, J = 8.6 Hz), 8.59-8.58 (1H, m). 497 495
    2-93
    Figure US20200087266A1-20200319-C00580
         		1H-NMR (DMSO-D6) δ: 0.33-0.37 (2H, m), 0.54-0.59 (2H, m), 1.23-1.30 (1H, m), 4.21 (2H, d, J = 7.3 Hz), 5.23 (2H, s), 6.97 (1H, d, J = 8.8 Hz), 7.23-7.35 (7H, m), 7.55 (1H, t, J = 8.0 Hz), 8.48 (1H, dd, J = 8.8, 2.2 Hz), 9.05 (1H, d, J = 2.2 Hz), 13.41 (1H, br s). 461 459
    2-94
    Figure US20200087266A1-20200319-C00581
         		1H-NMR (DMSO-D6) δ: 1.31 (9H, s), 5.38 (2H, s), 7.28 (1H, d, J = 8.2 Hz), 7.31 (1H, d, J = 8.2 Hz), 7.47-7.41 (2H, m), 7.52 (2H, d, J = 8.8 Hz), 7.56 (1H, t, J = 8.4 Hz), 7.86 (1H, td, J = 7.7, 1.5 Hz), 8.29 (2H, d, J = 8.8 Hz), 8.59 (1H, dd, J = 5.0, 0.8 Hz). 471 469
    2-95
    Figure US20200087266A1-20200319-C00582
         		1H-NMR (DMSO-D6) δ: 5.38 (2H, s), 7.39- 7.34 (2H, m), 7.46-7.43 (3H, m), 7.54 (1H, d, J = 7.9 Hz), 7.61-7.57 (2H, m), 7.66 (1H, d, J = 8.6 Hz), 7.72 (2H, d, J = 8.4 Hz), 7.82-7.76 (2H, m), 8.34 (2H, d, J = 8.4 Hz), 8.56 (1H, d, J = 4.9 Hz). 525 523
    2-96
    Figure US20200087266A1-20200319-C00583
         		1H-NMR (DMSO-D6) δ: 5.25 (2H, s), 7.36- 7.24 (8H, m), 7.46 (1H, ddd, J = 7.8, 4.9, 1.0 Hz), 7.57 (1H, t, J = 8.3 Hz), 7.72 (1H, dt, J = 7.8, 1.0 Hz), 7.79 (2H, d, J = 8.6 Hz), 7.90 (1H, td, J = 7.8, 1.8 Hz), 8.40 (2H, d, J = 8.6 Hz), 8.65 (1H, dq, J = 4.9, 0.9 Hz). 491 489
    2-97
    Figure US20200087266A1-20200319-C00584
         		1H-NMR (DMSO-D6) δ: 5.40 (2H, s), 7.30 (1H, dd, J = 8.2, 0.7 Hz), 7.33 (1H, d, J = 8.2 Hz), 7.51-7.43 (3H, m), 7.58 (1H, t, J = 8.2 Hz), 7.75 (1H, dt, J = 7.8, 1.0 Hz), 7.80 (2H, dd, J = 6.7, 1.9 Hz), 7.95-7.87 (2H, m), 8.40 (2H, dd, J = 6.7, 1.9 Hz), 8.61 (1H, d, J = 5.0 Hz), 8.66 (1H, dq, J = 5.0, 0.9 Hz). 492 490
    2-98
    Figure US20200087266A1-20200319-C00585
         		1H-NMR (DMSO-D6) δ: 2.48 (3H, s), 5.37 (2H, s), 7.23 (1H, d, J = 7.3 Hz), 7.28 (1H, d, J = 7.3 Hz), 7.48-7.45 (3H, m), 7.56 (1H, d, J = 7.9 Hz), 7.64-7.59 (2H, m), 7.67 (1H, d, J = 8.6 Hz), 7.82-7.72 (4H, m), 8.35 (2H, dd, J = 6.8, 2.0 Hz). 539 537
  • TABLE 2-12
    2-99
    Figure US20200087266A1-20200319-C00586
         		1H-NMR (DMSO-D6) δ: 2.41 (3H, s), 5.30 (2H, s), 7.09 (1H, d, J = 7.7 Hz), 7.14 (1H, d, J = 7.7 Hz), 7.51-7.45 (3H, m), 7.54 (1H, d, J = 7.7 Hz), 7.60 (1H, t, J = 7.7 Hz), 7.66-7.64 (3H, m), 7.78 (1H, d, J = 8.2 Hz), 7.81 (1H, dd, J = 8.2, 0.8 Hz), 8.61 (1H, dd, J = 8.2, 0.8 Hz), 9.40-9.39 (1H, m), 13.78 (1H, br s). 540 538
    2-100
    Figure US20200087266A1-20200319-C00587
         		1H-NMR (DMSO-D6) δ: 2.50 (3H, s), 5.41 (2H, s), 7.29-7.24 (1H, m), 7.35 (2H, dd, J = 4.9, 1.1 Hz), 7.43-7.39 (2H, m), 7.56 (2H, dd, J = 7.7, 4.2 Hz), 7.67 (1H, d, J = 8.6 Hz), 7.73 (2H, d, J = 8.6 Hz), 7.86-7.78 (2H, m), 8.36 (2H, d, J = 8.6 Hz), 8.59-8.57 (1H, m). 539 537
    2-101
    Figure US20200087266A1-20200319-C00588
         		1H-NMR (DMSO-D6) δ: 2.34 (3H, s), 5.41 (2H, s), 7.27 (1H, d, J = 7.7 Hz), 7.34 (1H, t, J = 7.7 Hz), 7.43-7.39 (4H, m), 7.56 (1H, d, J = 7.7 Hz), 7.68 (1H, d, J = 8.4 Hz), 7.72 (2H, dd, J = 6.7, 1.9 Hz), 7.87-7.78 (2H, m), 8.35 (2H, dd, J = 6.7, 1.9 Hz), 8.59 (1H, d, J = 4.4 Hz). 539 537
    2-102
    Figure US20200087266A1-20200319-C00589
         		1H-NMR (DMSO-D6) δ: 2.35 (3H, s), 5.40 (2H, s), 7.27 (2H, d, J = 7.9 Hz), 7.42-7.37 (2H, m), 7.49 (2H, d, J = 7.9 Hz), 7.56 (1H, d, J = 7.9 Hz), 7.67 (1H, d, J = 8.6 Hz), 7.71 (2H, d, J = 8.6 Hz), 7.85-7.77 (2H, m), 8.34 (2H, d, J = 8.6 Hz), 8.58 (1H, dq, J = 4.9, 0.9 Hz). 539 537
    2-103
    Figure US20200087266A1-20200319-C00590
         		1H-NMR (DMSO-D6) δ: 5.39 (2H, s), 7.39- 7.35 (2H, m), 7.56 (1H, d, J = 7.9 Hz), 7.69- 7.64 (2H, m), 7.72 (2H, dd, J = 6.7, 1.9 Hz), 7.83-7.75 (3H, m), 7.89-7.85 (2H, m), 8.39 (2H, dd, J = 6.7, 1.9 Hz), 8.57 (1H, d, J = 4.9 Hz). 593 591
    2-104
    Figure US20200087266A1-20200319-C00591
         		1H-NMR (DMSO-D6) δ: 5.40 (2H, s), 7.40- 7.36 (2H, m), 7.56 (1H, d, J = 7.9 Hz), 7.67 (1H, d, J = 8.6 Hz), 7.72 (1H, d, J = 7.9 Hz), 7.84-7.76 (5H, m), 7.92 (1H, d, J = 7.7 Hz), 7.99 (1H, br s), 8.37 (2H, dd, J = 6.8, 2.0 Hz), 8.57 (1H, dq, J = 4.9, 0.9 Hz). 593 591
    2-105
    Figure US20200087266A1-20200319-C00592
         		1H-NMR (DMSO-D6) δ: 5.40 (2H, s), 7.41- 7.36 (2H, m), 7.56 (1H, d, J = 7.9 Hz), 7.68 (1H, d, J = 8.6 Hz), 7.84-7.77 (8H, m), 8.37 (2H, dd, J = 6.7, 1.9 Hz), 8.57 (1H, d, J = 4.9 Hz). 593 591
    2-106
    Figure US20200087266A1-20200319-C00593
         		1H-NMR (DMSO-D6) δ: 5.40 (2H, s), 7.41- 7.36 (2H, m), 7.51-7.45 (3H, m), 7.57 (1H, d, J = 7.7 Hz), 7.64-7.60 (2H, m), 7.68 (1H, d, J = 8.4 Hz), 7.84-7.79 (3H, m), 8.10 (1H, dd, J = 10.5, 1.4 Hz), 8.19 (1H, dd, J = 8.2, 1.5 Hz), 8.57 (1H, dq, J = 4.9, 0.9 Hz). 543 541
    2-107
    Figure US20200087266A1-20200319-C00594
         		1H-NMR (DMSO-D6) δ: 2.54 (3H, s), 5.45 (2H, s), 7.30 (1H, td, J = 7.6, 1.1 Hz), 7.44- 7.35 (3H, m), 7.56-7.50 (1H, m), 7.58 (1H, d, J = 7.9 Hz), 7.71-7.67 (2H, m), 7.75 (2H, dd, J = 6.7, 1.9 Hz), 7.81 (1H, t, J = 7.9 Hz), 7.92 (1H, br s), 8.36 (2H, dd, J = 6.7, 1.9 Hz). 557 555
  • TABLE 2-13
    2-108
    Figure US20200087266A1-20200319-C00595
         		1H-NMR (DMSO-D6) δ: 2.52 (3H, s), 5.43 (2H, s), 7.33 (1H, d, J = 7.7 Hz), 7.38 (1H, d, J = 7.7 Hz), 7.43 (1H, td, J = 7.7, 1.4 Hz), 7.48 (1H, td, J = 7.7, 1.9 Hz), 7.57 (1H, d, J = 8.0 Hz), 7.63 (1H, dd, J = 8.0, 1.2 Hz), 7.68 (1H, d, J = 8.3 Hz), 7.76-7.72 (3H, m), 7.81 (1H, t, J = 8.3 Hz), 7.90-7.84 (1H, m), 8.37 (2H, dd, J = 6.7, 1.9 Hz). 573 571
    2-109
    Figure US20200087266A1-20200319-C00596
         		1H-NMR (DMSO-D6) δ: 2.51 (3H, s), 3.88 (3H, s), 5.41 (2H, s), 7.00 (1H, td, J = 7.5, 0.9 Hz), 7.12 (1H, d, J = 7.9 Hz), 7.29 (1H, d, J = 7.9 Hz), 7.34 (1H, d, J = 7.5 Hz), 7.43 (1H, ddd, J = 8.8, 7.1, 1.3 Hz), 7.52 (1H, dd, J = 7.5, 1.7 Hz), 7.57 (1H, d, J = 7.9 Hz), 7.69- 7.66 (3H, m), 7.85-7.78 (2H, m), 8.34 (2H, dd, J = 6.8, 2.0 Hz). 569 567
    2-110
    Figure US20200087266A1-20200319-C00597
         		1H-NMR (DMSO-D6) δ: 2.24 (3H, s), 2.49 (3H, s), 5.41 (2H, s), 7.22 (1H, br s), 7.31 (1H, br s), 7.46-7.43 (3H, m), 7.60-7.57 (3H, m), 7.72-7.68 (3H, m), 7.82 (1H, t, J = 8.3 Hz), 8.33 (2H, dd, J = 6.7, 1.8 Hz). 553 551
    2-111
    Figure US20200087266A1-20200319-C00598
         		1H-NMR (DMSO-D6) δ: 5.42 (2H, d, J = 1.6 Hz), 7.42-7.48 (4H, m), 7.54 (1H, t, J = 4.2 Hz), 7.59-7.63 (2H, m), 7.64-7.73 (3H, m), 7.81 (2H, d, J = 4.2 Hz), 8.29 (2H, d, J = 8.6 Hz), 8.35-8.37 (1H, m). 543 541
    2-112
    Figure US20200087266A1-20200319-C00599
         		1H-NMR (DMSO-D6) δ: 5.37 (2H, s), 7.37 (1H, d, J = 8.3 Hz), 7.44-7.48 (3H, m), 7.55 (1H, d, J = 7.6 Hz), 7.58-7.66 (3H, m), 7.73 (2H, d, J = 8.6 Hz), 7.79 (1H, t, J = 8.1 Hz), 7.88 (1H, dd, J = 8.6, 2.3 Hz), 8.34 (2H, d, J = 8.6 Hz), 8.59 (1H, d, J = 2.3 Hz). 559 557
    2-113
    Figure US20200087266A1-20200319-C00600
         		1H-NMR (DMSO-D6) δ: 2.27 (3H, s), 5.43 (2H, s), 7.35 (1H, s), 7.39 (1H, d, J = 5.8 Hz), 7.44-7.48 (3H, m), 7.57-7.63 (3H, m), 7.69- 7.75 (3H, m), 7.83 (1H, t, J = 8.1 Hz), 8.35 (2H, d, J = 8.3 Hz), 8.51 (1H, d, J = 5.3 Hz). 539 537
    2-114
    Figure US20200087266A1-20200319-C00601
         		1H-NMR (DMSO-D6) δ: 7.36 (1H, d, J = 7.9 Hz), 7.44-7.48 (3H, m), 7.56 (1H, d, J = 7.9 Hz), 7.59-7.63 (2H, m), 7.66-7.75 (4H, m), 7.79 (1H, t, J = 8.1 Hz), 8.34 (2H, d, J = 8.6 Hz), 8.44 (1H, s). 539 537
    2-115
    Figure US20200087266A1-20200319-C00602
         		1H-NMR (DMSO-D6) δ: 5.36 (2H, s), 7.41 (1H, dd, J = 8.8, 4.4 Hz), 7.44-7.48 (3H, m), 7.55 (1H, d, J = 7.9 Hz), 7.58-7.63 (2H, m), 7.65-7.75 (4H, m), 7.79 (1H, t, J = 8.1 Hz), 8.34 (2H, d, J = 8.6 Hz), 8.53 (1H, d, J = 3.0 Hz). 543 541
    2-116
    Figure US20200087266A1-20200319-C00603
         		1H-NMR (DMSO-D6) δ: 2.53 (3H, s), 5.44 (2H, s), 7.54-7.30 (6H, m), 7.58 (1H, d, J = 7.7 Hz), 7.69 (1H, d, J = 8.0 Hz), 7.74 (2H, dd, J = 6.8, 1.8 Hz), 7.81 (1H, t, J = 8.0 Hz), 7.90 (1H, br s), 8.35 (2H, dd, J = 6.8, 1.8 Hz). 557 555
  • TABLE 2-14
    2-117
    Figure US20200087266A1-20200319-C00604
         		1H-NMR (DMSO-D6) δ: 2.51 (3H, s), 5.42 (2H, s), 7.33-7.29 (3H, m), 7.37 (1H, d, J = 7.9 Hz), 7.57 (1H, d, J = 7.9 Hz), 7.69-7.65 (3H, m), 7.72 (2H, dd, J = 6.8, 1.8 Hz), 7.89- 7.78 (2H, m), 8.34 (2H, dd, J = 6.8, 1.8 Hz). 557 555
    2-118
    Figure US20200087266A1-20200319-C00605
         		1H-NMR (CDCl3) δ: 3.87 (3H, s), 5.27 (2H, s), 6.61 (1H, d, J = 8.2 Hz), 6.86 (1H, d, J = 7.3 Hz), 7.31 (1H, d, J = 8.6 Hz), 7.35-7.39 (3H, m), 7.43-7.51 (2H, m), 7.53-7.60 (3H, m), 7.64 (2H, d, J = 8.4 Hz), 8.50 (2H, d, J = 8.4 Hz). 555 553
    2-119
    Figure US20200087266A1-20200319-C00606
         		1H-NMR (DMSO-D6) δ: 5.10 (2H, s), 6.16- 6.32 (2H, m), 7.32-7.38 (1H, m), 7.44-7.48 (3H, m), 7.54-7.63 (4H, m), 7.70-7.75 (2H, m), 7.77-7.84 (1H, m), 8.34 (2H, d, J = 8.6 Hz). 541 539
    2-120
    Figure US20200087266A1-20200319-C00607
         		1H-NMR (DMSO-D6) δ: 3.89 (3H, s), 5.48 (2H, s), 7.20-7.26 (2H, m), 7.44-7.49 (3H, m), 7.58-7.64 (3H, m), 7.69-7.75 (3H, m), 7.84 (1H, t, J = 8.2 Hz), 8.33 (2H, d, J = 8.6 Hz), 8.56 (1H, d, J = 6.0 Hz). 555 553
    2-121
    Figure US20200087266A1-20200319-C00608
         		1H-NMR (DMSO-D6) δ: 5.47 (2H, s), 7.44- 7.48 (3H, m), 7.55-7.63 (4H, m), 7.68 (1H, d, J = 8.6 Hz), 7.73 (2H, d, J = 8.6 Hz), 7.78- 7.84 (2H, m), 8.03 (1H, t, J = 8.1 Hz), 8.35 (2H, d, J = 8.6 Hz), 13.58 (1H, br s). 593 591
    2-122
    Figure US20200087266A1-20200319-C00609
         		1H-NMR (DMSO-D6) δ: 5.47 (2H, s), 7.44- 7.51 (4H, m), 7.56-7.63 (3H, m), 7.67-7.74 (4H, m), 7.82 (1H, t, J = 8.1 Hz), 8.34 (2H, d, J = 8.6 Hz), 8.83 (1H, d, J = 5.1 Hz). 593 591
    2-123
    Figure US20200087266A1-20200319-C00610
         		1H-NMR (DMSO-D6) δ: 2.50 (3H, s), 5.40 (2H, s), 7.29 (1H, d, J = 7.7 Hz), 7.33 (1H, d, J = 7.7 Hz), 7.59-7.46 (4H, m), 7.68 (1H, d, J = 8.4 Hz), 7.71-7.69 (1H, m), 7.75 (2H, dd, J = 6.7, 1.9 Hz), 7.84-7.78 (2H, m), 8.36 (2H, dd, J = 6.7, 1.9 Hz). 573 571
    2-124
    Figure US20200087266A1-20200319-C00611
         		1H-NMR (DMSO-D6) δ: 2.49 (3H, s), 5.39 (2H, s), 7.26 (1H, d, J = 7.5 Hz), 7.31 (1H, d, J = 7.5 Hz), 7.53 (2H, dt, J = 8.7, 2.2 Hz), 7.56 (1H, d, J = 7.9 Hz), 7.63 (2H, dt, J = 8.7, 2.2 Hz), 7.67 (1H, d, J = 8.4 Hz), 7.74 (2H, dd, J = 6.7, 1.9 Hz), 7.83-7.76 (2H, m), 8.35 (2H, dd, J = 6.7, 1.9 Hz). 573 571
    2-125
    Figure US20200087266A1-20200319-C00612
         		1H-NMR (DMSO-D6) δ: 2.50 (3H, s), 3.80 (3H, s), 5.40 (2H, s), 7.03 (1H, dq, J = 8.6, 1.2 Hz), 7.19-7.14 (2H, m), 7.29 (1H, d, J = 7.3 Hz), 7.39-7.32 (2H, m), 7.57 (1H, d, J = 7.9 Hz), 7.67 (1H, d, J = 8.6 Hz), 7.73 (2H, dd, J = 6.7, 1.9 Hz), 7.85-7.77 (2H, m), 8.34 (2H, dd, J = 6.7, 1.9 Hz). 569 567
  • TABLE 2-15
    2-126
    Figure US20200087266A1-20200319-C00613
         		1H-NMR (DMSO-D6) δ: 2.49 (3H, s), 3.81 (3H, s), 5.40 (2H, s), 7.01 (2H, dt, J = 9.5, 2.4 Hz), 7.28 (1H, d, J = 6.8 Hz), 7.32 (1H, d, J = 7.5 Hz), 7.57-7.53 (3H, m), 7.70-7.66 (3H, m), 7.80 (2H, t, J = 7.9 Hz), 8.33 (2H, dd, J = 6.8, 2.0 Hz). 569 567
    2-127
    Figure US20200087266A1-20200319-C00614
         		1H-NMR (DMSO-D6) δ: 2.35 (3H, s), 2.51 (3H, s), 5.42 (2H, s), 7.27 (2H, d, J = 7.7 Hz), 7.32 (1H, d, J = 7.7 Hz), 7.36 (1H, d, J = 7.7 Hz), 7.49 (2H, d, J = 8.2 Hz), 7.57 (1H, d, J = 7.7 Hz), 7.72-7.66 (3H, m), 7.88-7.77 (2H, m), 8.33 (2H, dd, J = 6.7, 1.9 Hz). 553 551
    2-128
    Figure US20200087266A1-20200319-C00615
         		1H-NMR (DMSO-D6) δ: 1.19 (3H, t, J = 7.5 Hz), 2.52 (3H, s), 2.65 (2H, q, J = 7.6 Hz), 5.43 (2H, s), 7.30 (2H, d, J = 8.4 Hz), 7.34 (1H, d, J = 7.7 Hz), 7.39 (1H, d, J = 7.7 Hz), 7.52 (2H, d, J = 8.4 Hz), 7.57 (1H, d, J = 7.7 Hz), 7.72-7.67 (3H, m), 7.81 (1H, t, J = 8.3 Hz), 7.87 (1H, br s), 8.33 (2H, dd, J = 6.7, 1.9 Hz). 567 565
    2-129
    Figure US20200087266A1-20200319-C00616
         		1H-NMR (DMSO-D6) δ: 2.51 (3H, s), 5.42 (2H, s), 7.32 (1H, d, J = 7.1 Hz), 7.37 (1H, d, J = 7.7 Hz), 7.63-7.48 (4H, m), 7.72-7.67 (3H, m), 7.89-7.77 (3H, m), 8.37 (2H, dd, J = 6.8, 2.0 Hz). 623 621
    2-130
    Figure US20200087266A1-20200319-C00617
         		1H-NMR (DMSO-D6) δ: 2.55 (3H, s), 5.47 (2H, s), 7.40 (1H, d, J = 7.1 Hz), 7.48-7.43 (3H, m), 7.58 (1H, d, J = 7.7 Hz), 7.69 (1H, d, J = 8.5 Hz), 7.77-7.72 (4H, m), 7.82 (1H, t, J = 8.5 Hz), 7.95 (1H, br s), 8.35 (2H, dd, J = 6.7, 1.9 Hz). 623 621
  • TABLE 3-1
    Ex- MS MS
    ample Structure NMR (M + H) (M − H) Note
    3-1
    Figure US20200087266A1-20200319-C00618
         		1H-NMR (DMSO-D6) δ: 2.19 (6H, s), 2.30 (3H, s), 7.01 (2H, s), 7.90 (2H, d, J = 8.4 Hz), 8.51 (2H, d, J = 8.4 Hz), 13.26 (1H, br s). 360 358
    3-2
    Figure US20200087266A1-20200319-C00619
         		1H-NMR (DMSO-D6) δ: 3.72 (3H, s), 4.59 (2H, d, J = 5.5 Hz), 5.41 (1H, t, J = 5.5 Hz), 7.45 (1H, d, J = 8.4 Hz), 7.67 (1H, d, J = 8.4 Hz), 7.92 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.70 (1H, br s). 412 410
    3-3
    Figure US20200087266A1-20200319-C00620
         		1H-NMR (DMSO-D6) δ: 2.31 (3H, s), 3.71 (3H, s), 7.36 (1H, d, J = 8.4 Hz), 7.49 (1H, d, J = 8.4 Hz), 7.93 (2H, d, J = 8.3 Hz), 8.53 (2H, d, J = 8.3 Hz), 13.67 (1H, br s). 396 394
    3-4
    Figure US20200087266A1-20200319-C00621
         		1H-NMR (DMSO-D6) δ: 1.09 (3H, t, J = 7.0 Hz), 2.30 (3H, s), 3.94 (2H, q, J = 7.0 Hz), 7.34 (1H, d, J = 8.2 Hz), 7.48 (1H, d, J = 8.2 Hz), 7.93 (2H, d, J = 8.2 Hz), 8.53 (2H, d, J = 8.2 Hz), 13.66 (1H, br s). 410 408
    3-5
    Figure US20200087266A1-20200319-C00622
         		1H-NMR (DMSO-D6) δ: 1.91 (3H, s), 3.74 (3H, s), 4.32 (2H, d, J = 5.8 Hz), 7.43 (1H, d, J = 8.4 Hz), 7.49 (1H, d, J = 8.4 Hz), 7.92 (2H, d, J = 8.4 Hz), 8.42 (1H, t, J = 5.8 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.73 (1H, br s). 453 451
    3-6
    Figure US20200087266A1-20200319-C00623
         		1H-NMR (DMSO-D6) δ: 2.17 (3H, s), 4.55 (2H, d, J = 5.1 Hz), 5.35 (1H, t, J = 5.2 Hz), 7.48 (1H, d, J = 8.4 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.91 (2H, d, J = 8.6 Hz), 8.51 (2H, d, J = 8.6 Hz), 13.61 (1H, br s). 396 394
    3-7
    Figure US20200087266A1-20200319-C00624
         		1H-NMR (DMSO-D6) δ: 1.90 (3H, s), 2.21 (3H, s), 4.28 (2H, d, J = 5.7 Hz), 7.41 (1H, d, J = 8.4 Hz), 7.47 (1H, d, J = 8.4 Hz), 7.91 (2H, d, J = 8.2 Hz), 8.37 (1H, t, J = 5.8 Hz), 8.51 (2H, d, J = 8.2 Hz), 13.62 (1H, br s). 437 435
    3-8
    Figure US20200087266A1-20200319-C00625
         		1H-NMR (DMSO-D6) δ: 1.16 (9H, s), 2.21 (3H, s), 4.27 (2H, d, J = 5.7 Hz), 7.34 (1H, d, J = 8.4 Hz), 7.46 (1H, d, J = 8.4 Hz), 7.91 (2H, d, J = 8.2 Hz), 8.09 (1H, t, J = 5.6 Hz), 8.51 (2H, d, J = 8.2 Hz), 13.64 (1H, br s). 479 477
  • TABLE 3-2
    3-9 
    Figure US20200087266A1-20200319-C00626
         		1H-NMR (DMSO-D6) δ: 2.32 (3H, s), 3.47 (2H, t, J = 4.9 Hz), 3.88 (2H, t, J = 4.8 Hz), 4.70 (1H, br s), 7.34 (1H, d, J = 8.1 Hz), 7.47 (1H, d, J = 8.1 Hz), 7.92 (2H, d, J = 8.4 Hz), 8.51 (2H, d, J = 8.4 Hz), 13.56 (1H, br s). 426 424
    3-10
    Figure US20200087266A1-20200319-C00627
         		1H-NMR (DMSO-D6) δ: 1.33 (9H, s), 2.22 (3H, s), 4.30 (2H, d, J = 6.0 Hz), 7.52-7.54 (2H, m), 7.60 (1H, d, J = 8.4 Hz), 7.91 (2H, d, J = 8.4 Hz), 8.51 (2H, d, J = 8.4 Hz), 13.65 (1H, br s). 515 513
    3-11
    Figure US20200087266A1-20200319-C00628
         		1H-NMR (DMSO-D6) δ: 3.79-3.87 (3H, m), 7.28 (1H, dd, J = 9.3, 3.7 Hz), 7.67 (1H, t, J = 9.2 Hz), 7.93 (2H, d, J = 8.6 Hz), 8.51 (2H, d, J = 8.6 Hz), 13.68 (1H, br s). 400 398
    3-12
    Figure US20200087266A1-20200319-C00629
         		1H-NMR (DMSO-D6) δ: 3.82 (3H, s), 5.22 (2H, s), 7.17 (2H, d, J = 9.0 Hz), 7.24-7.27 (1H, br m), 7.35 (1H, t, J = 7.2 Hz), 7.41 (2H, t, J = 7.3 Hz), 7.47 (2H, d, J = 7.1 Hz), 7.63- 7.66 (1H, br m), 8.28 (2H, d, J = 9.0 Hz), 13.26 (1H, br s). 438 436
    3-13
    Figure US20200087266A1-20200319-C00630
         		1H-NMR (DMSO-D6) δ: 2.36 (3H, s), 5.01 (2H, s), 7.11-7.15 (1H, m), 7.22 (1H, d, J = 7.7 Hz), 7.39 (1H, d, J = 8.4 Hz), 7.52 (1H, d, J = 8.4 Hz), 7.60 (1H, td, J = 7.7, 1.7 Hz), 7.86 (2H, d, J = 8.4 Hz), 8.31 (1H, d, J = 4.0 Hz), 8.41 (2H, d, J = 8.4 Hz), 13.61 (1H, br s). 473 471
    3-14
    Figure US20200087266A1-20200319-C00631
         		1H-NMR (DMSO-D6) δ: 0.88 (6H, d, J = 6.6 Hz), 1.41 (6H, s), 1.83-1.95 (1H, m), 2.19 (3H, s), 2.54 (2H, d, J = 7.3 Hz), 4.33 (2H, d, J = 5.5 Hz), 7.28-7.38 (3H, m), 7.46 (1H, d, J = 8.4 Hz), 8.24 (2H, d, J = 8.2 Hz), 8.52-8.60 (1H, m), 13.35 (1H, br s). 521 519
    3-15
    Figure US20200087266A1-20200319-C00632
         		1H-NMR (DMSO-D6) δ: 0.88 (6H, d, J = 6.6 Hz), 1.18 (3H, t, J = 7.1 Hz), 1.83-1.94 (1H, m), 2.19 (3H, s), 2.54 (2H, d, J = 7.1 Hz), 4.02 (2H, q, J = 7.1 Hz), 4.21 (2H, d, J = 5.7 Hz), 7.33 (2H, d, J = 8.4 Hz), 7.39 (1H, d, J = 8.2 Hz), 7.46 (1H, d, J = 8.2 Hz), 7.69-7.75 (1H, m), 8.24 (2H, d, J = 8.4 Hz), 13.33 (1H, br s). 455 453
    3-16
    Figure US20200087266A1-20200319-C00633
         		1H-NMR (DMSO-D6) δ: 0.91 (3H, t, J = 7.3 Hz), 1.37 (6H, s), 1.60-1.67 (2H, m), 2.33 (3H, s), 2.62 (2H, t, J = 7.6 Hz), 4.31 (2H, d, J = 5.7 Hz), 7.30-7.38 (3H, m), 7.52 (1H, s), 8.10-8.15 (2H, m), 8.48 (1H, t, J = 5.7 Hz). 507 505
  • TABLE 3-3
    3-17
    Figure US20200087266A1-20200319-C00634
         		1H-NMR (DMSO-D6) δ: 0.35 (2H, dt, J = 8.0, 2.9 Hz), 0.59 (2H, ddd, J = 9.1, 5.0, 2.9 Hz), 1.20-1.29 (1H, m), 1.38 (6H, s), 2.35 (3H, s), 3.93 (2H, d, J = 7.0 Hz), 4.32 (2H, d, J = 5.6 Hz), 7.05 (2H, d, J = 8.8 Hz), 7.44 (1H, s), 7.56 (1H, s), 8.29 (2H, d, J = 8.8 Hz), 8.49 (1H, t, J = 5.6 Hz), 13.08 (1H, br s). 535 533
    3-18
    Figure US20200087266A1-20200319-C00635
         		1H-NMR (DMSO-D6) δ: 0.99 (6H, d, J = 6.6 Hz), 1.41 (6H, s), 1.98-2.10 (1H, m), 2.18 (3H, s), 3.85 (2H, d, J = 6.6 Hz), 4.32 (2H, d, J = 5.7 Hz), 7.06 (2H, d, J = 8.4 Hz), 7.30 (1H, d, J = 8.2 Hz), 7.44 (1H, d, J = 8.2 Hz), 8.27 (2H, d, J = 8.4 Hz), 8.52-8.59 (1H, m), 13.23 (1H, br s). 537 535
    3-19
    Figure US20200087266A1-20200319-C00636
         		1H-NMR (DMSO-D6) δ: 0.35 (2H, td, J = 5.2, 4.1 Hz), 0.57-0.62 (2H, m), 1.24-1.27 (1H, m), 2.61 (3H, s), 3.94 (2H, d, J = 7.2 Hz), 7.09 (2H, d, J = 8.8 Hz), 7.64 (1H, s), 8.27 (1H, s), 8.30 (2H, d, J = 8.8 Hz), 13.20 (2H, br s). 412 410
    3-20
    Figure US20200087266A1-20200319-C00637
         		1H-NMR (DMSO-D6) δ: 0.35 (2H, td, J = 5.1, 4.1 Hz), 0.58-0.60 (2H, m), 1.22-1.30 (1H, m), 2.45 (3H, s), 3.94 (2H, d, J = 7.2 Hz), 7.09 (2H, d, J = 9.1 Hz), 7.56 (2H, s), 7.81 (1H, s), 7.85 (1H, s), 8.32 (2H, d, J = 8.8 Hz), 13.06 (1H, br s). 411 409
    3-21
    Figure US20200087266A1-20200319-C00638
         		1H-NMR (DMSO-D6) δ: 0.35 (2H, td, J = 5.2, 4.0 Hz), 0.57-0.62 (2H, m), 1.22-1.27 (1H, m), 2.42 (3H, s), 2.77 (3H, d, J = 4.7 Hz), 3.94 (2H, d, J = 7.0 Hz), 7.09 (2H, d, J = 9.1 Hz), 7.56 (1H, s), 7.77 (1H, s), 8.31 (2H, d, J = 9.1 Hz), 8.32 (1H, s), 13.09 (1H, br s). 425 423
    3-22
    Figure US20200087266A1-20200319-C00639
         		1H-NMR (DMSO-D6) δ: 0.35 (2H, td, J = 5.6, 4.4 Hz), 0.57-0.61 (2H, m), 1.21-1.29 (1H, m), 2.28 (3H, s), 2.81 (3H, s), 3.02 (3H, s), 3.93 (2H, d, J = 7.2 Hz), 7.08 (2H, d, J = 9.1 Hz), 7.59 (1H, s), 7.62 (1H, s), 8.30 (2H, d, J = 9.1 Hz), 13.10 (1H, br s). 439 437
    3-23
    Figure US20200087266A1-20200319-C00640
         		1H-NMR (DMSO-D6) δ: 0.35 (2H, td, J = 5.3, 4.0 Hz), 0.57-0.61 (2H, m), 0.90 (3H, t, J = 7.3 Hz), 1.20-1.30 (2H, m), 1.30-1.40 (2H, m), 1.46-1.53 (2H, m), 2.41 (3H, s), 3.24 (2H, q, J = 6.9 Hz), 3.94 (2H, d, J = 7.2 Hz), 7.09 (2H, d, J = 9.1 Hz), 7.56 (1H, s), 7.74 (1H, s), 8.31 (2H, d, J = 9.1 Hz), 8.37 (1H, t, J = 5.5 Hz), 13.09 (1H, br s). 467 465
    • Experimental Example 1 Evaluation of Human mPGES-1 Enzyme Inhibitory Activity
  • The human mPGES-1 enzyme inhibitory activity of a test article was evaluated according to the report of Xu et al. (XU, D et al. MF63 [2-(6-chloro-1H-phenanthro[9,10-d]imidazol-2-yl)-isophthalonitrile], a selective microsomal prostaglandin E synthase-1 inhibitor, relieves pyresis and pain in preclinical models of inflammation. J Pharmacol Exp Ther. September 2008, Vol. 326, No. 3, pages 754-763). That is, the amount of PGE2 produced by human mPGES-1 in the presence of a test article was measured by the HTRF (homogeneous time resolved fluorescence) method, based on which the human mPGES-1 enzyme inhibitory activity of the test article was determined.
  • 1) Preparation of Human mPGES-1 Expressing Cell Microsome Fraction
  • A DNA fragment containing human mPGES-1, which is added with a BamHI recognition cleavage sequence immediately before the translation initiation codon and an EcoRI recognition cleavage sequence immediately after the translation termination codon was amplified by the PCR (Polymerase Chain Reaction) method using a human mPGES-1 expression plasmid DNA (pME-18S/iPGES-1) prepared in-house as a template. The purified DNA fragment was digested with BamHI and EcoRI, and ligated to pcDNA3.1(+) (Invitrogen, model number V790-20), similarly digested with BamHI and EcoRI, by using a DNA Ligation kit ver. 2.1 (Takara Bio, model number 6022). The human mPGES-1 expression plasmid DNA was isolated from Escherichia coli DH5α (TOYOBO, model number DNA-903) transformed with the obtained ligation product. The base sequence of human mPGES-1 cloned to a vector was determined by the Dye Terminator method by using BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, #4337455). The determined sequence was identical with the sequence of the protein translational region of human mPGES-1 (Accession number NM 004878) registered in the NCBI Reference Database.
  • Human mPGES-1 expression plasmid DNA was transfected into Chinese hamster ovary-derived cells (FreeStyle CHO-S Cell, Invitrogen, #R800-07) by using a transgene reagent (FreeStyle MAX Reagent (Invitrogen, #16447-100)), and cultured with shaking (8% CO2, 37° C.) in a medium containing 8 mmol/L L-glutamine (GIBCO FreeStyle CHO Expression Medium, Invitrogen, #12651-022) for 48 hr.
  • The CHO-S cells were suspended in Homogenate Buffer (100 mmol/L potassium phosphate (pH 7.4), 250 mmol/L Sucrose, 100 mmol/L EDTA, complete EDTA free (Roche, #1873580)). Using an ultrasonic disruptor UD-201 (Tomy Seiko), the suspended cells were disrupted at output:3, duty cycle:50 for 30 seconds. The precipitate was removed by centrifugation (1,000×g, 5 min, 4° C.), and the supernatant was centrifuged (5,000×g, 10 min, 4° C.) The supernatant was further centrifuged (105,000×g, 60 min, 4° C.). The obtained precipitate was suspended in Resuspension Buffer (100 mmol/L potassium phosphate (pH 7.4), 250 mmol/L sucrose, 100 mmol/L EDTA, 10% glycerol) to give a microsome fraction.
  • The protein concentration of the microsome fraction was measured by the Bradford method (Protein Assay Kit, Bio-Rad). The microsome fraction was rapidly frozen in liquid nitrogen and preserved at −80° C. Human mPGES-1 in the microsome fraction was detected by Western Blot using rabbit anti-mPGES-1 polyclonal antibody (ThermoFisher Scientific, #PA1-10264).
  • 2) Evaluation of Human mPGES-1 Enzyme Inhibitory Activity
  • A test article solution diluted with 0.1 mol/L potassium phosphate, pH 7.4 (hereinafter to be referred to as KPB) or DMSO (Nacalai Tesque, #13407-45) was added at 5 μL/well to 96 well V-bottom plate (Corning, #3363). The final DMSO concentration during the reaction was set to 2%(v/v). Furthermore, a microsome fraction of CHO-S cells expressing human mPGES-1, which was diluted with reduced GSH (12.5 mmol/L KPB solution, SIGMA, #G6529-25G) such that the protein concentration was 5 μg/mL, was added at 20 μL/well. The amount of the microsome fraction used is the amount of microsome fraction within a range where the amount of PGE2 produced under the reaction conditions shown below and the amount of microsome fraction used show linearity. To the blank was added reduced GSH (12.5 mmol/L KPB solution) at 20 μL/well. After stirring at room temperature for 10 min, PGH2 (PGH2 dissolved in cold acetone to 100 μg/mL and diluted with D-PBS(−) (Nikken biomedical laboratory, #CM6201) to 10 μg/mL, Cayman Chemical, #17020) was added at 25 μL/well, and the mixture was stood at room temperature for 45 seconds. Tin(II) chloride dihydrate (2 mg/mL 10 mmol/L citric acid solution, Wako Pure Chemical Industries, Ltd., #204-01562) was added at 50 μL/well, and the plate was gently shaken to discontinue the enzyme reaction.
  • The concentration of PGE2 in the above-mentioned enzyme reaction mixture was measured using Prostaglandin E2 assay (CISbio Bioassays, #62P2APEC) according to the manual. As the reference standard for analytical curve, PGE2 (Cayman Chemical, #14010) was used. Using RUBYstar (BMG Labtech), the time-resolved fluorescence at 620 nm and 665 nm relative to the excitation light at 337 nm was measured. PGE2 concentration was extrapolated from the PGE2 analytical curve. Average of the PGE2 concentrations of the respectively-treated wells was used as the data.
  • The mPGES-1 enzyme inhibitory activity (%) of the test article was calculated according to the following formula 1.

  • mPGES-1 enzyme inhibitory activity (%)=(PGE2A−PGE2X)/(PGE2A−PGE2B)×100   [formula 1]
    • PGE2A: PGE2 concentration of vehicle-treated well
    • PGE2B: PGE2 concentration of blank well
    • PGE2X: PGE2 concentration of test article-treated well
  • The IC50 value (50% inhibitory concentration) of the test article was calculated according to the following formula 2.

  • IC50 value=10{log 10(D/E×(50−G)/(F−G)−log 10(E)}  [formula 2]
    • D: concentration of test article that shows activity of not less than 50% inhibition between two points across 50% inhibition
    • E: concentration of test article that shows activity of not more than 50% inhibition between two points across 50% inhibition
    • F: mPGES-1 enzyme inhibitory activity (%) when concentration of test article is D
    • G: mPGES-1 enzyme inhibitory activity (%) when concentration of test article is E
  • The results are shown in Table 4-1 to Table 4-9.
  • TABLE 4-1
    Human mPGES-1
    enzyme inhibitory
    Example activity (μM)
    1-1 0.813
    1-2 0.138
    1-3 0.164
    1-4 0.025
    1-5 0.672
    1-6 0.163
    1-7 0.652
    1-8 27.0
    1-9 0.601
    1-10 5% inhibition
    (at 30 μM)
    1-11 42% inhibition
    (at 30 μM)
    1-12 0.015
    1-13 0.397
    1-14 1.413
    1-15 0.0074
    1-16 0.010
    1-17 0.735
    1-18 0.114
    1-19 0.411
    1-20 0.0016
    1-21 0.988
    1-22 0.0027
    1-23 0.134
    1-24 0.0006
    1-25 0.108
    1-26 0.018
    1-27 0.0010
    1-28 0.0006
    1-29 0.0011
    1-30 0.0006
    1-31 0.0010
    1-32 0.0007
    1-33 0.0008
    1-34 0.0059
    1-35 0.022
    1-36 0.0007
    1-37 0.0008
    1-38 0.0015
    1-39 0.0019
    1-40 2.231
    1-41 0.0023
    1-42 0.0010
    1-43 0.0020
    1-44 0.0006
    1-45 0.138
    1-46 0.0007
    1-47 0.043
    1-48 0.0009
    1-49 0.0009
    1-50 0.0009
    1-51 0.0010
  • TABLE 4-2
    Human mPGES-1
    enzyme inhibitory
    Example activity (μM)
    1-52 0.0006
    1-53 0.0045
    1-54 0.0009
    1-55 0.0011
    1-56 0.0006
    1-57 0.0005
    1-58 0.0005
    1-59 0.0006
    1-60 0.0004
    1-61 0.0007
    1-62 0.0010
    1-63 0.0005
    1-64 0.0019
    1-65 0.0086
    1-66 0.0041
    1-67 0.0010
    1-68 0.0003
    1-69 0.0003
    1-70 0.0007
    1-71 0.0025
    1-72 0.0013
    1-73 0.0006
    1-74 0.0006
    1-75 0.0067
    1-76 0.0017
    1-77 0.0009
    1-78 0.0022
    1-79 0.0012
    1-80 0.0031
    1-81 0.0006
    1-82 0.0008
    1-83 0.011
    1-84 0.0006
    1-85 0.0005
    1-86 0.0058
    1-87 0.0008
    1-88 0.0012
    1-89 0.0009
    1-90 0.0004
    1-91 0.0004
    1-92 0.0005
    1-93 0.0007
    1-94 0.0046
    1-95 0.0021
    1-96 0.081
    1-97 0.0091
    1-98 0.0009
    1-99 0.0007
    1-100 0.0009
    1-101 0.0058
    1-102 0.0009
  • TABLE 4-3
    Human mPGES-1
    enzyme inhibitory
    Example activity (μM)
    1-103 0.0013
    1-104 0.0015
    1-105 0.0007
    1-106 0.0007
    1-107 0.0012
    1-108 0.0006
    1-109 0.0025
    1-110 0.0009
    1-111 0.0012
    1-112 0.0009
    1-113 0.0009
    1-114 0.0059
    1-115 0.0006
    1-116 0.0020
    1-117 0.0016
    1-118 0.0019
    1-119 0.0010
    1-120 0.048
    1-121 0.0012
    1-122 0.0013
    1-123 0.0023
    1-124 0.0009
    1-125 0.0008
    1-126 0.0008
    1-127 0.0008
    1-128 0.0013
    1-129 0.0006
    1-130 0.009
    1-131 0.0009
    1-132 0.0003
    1-133 0.0005
    1-134 0.004
    1-135 0.0005
    1-136 0.0005
    1-137 0.0005
    1-138 0.0005
    1-139 0.0006
    1-140 0.0005
    1-141 0.0011
    1-142 0.0005
    1-143 0.0010
    1-144 0.0009
    1-145 0.0005
    1-146 0.0004
    1-147 0.0008
    1-148 0.0017
    1-149 0.0008
    1-150 0.0004
    1-151 0.0004
    1-152 0.0004
    1-153 0.0005
  • TABLE 4-4
    Human mPGES-1
    enzyme inhibitory
    Example activity (μM)
    1-154 0.0005
    1-155 0.0035
    1-156 0.0041
    1-157 0.0007
    1-158 0.012
    1-159 0.0007
    1-160 0.0014
    1-161 0.0013
    1-162 0.0012
    1-163 0.0010
    1-164 0.0037
    1-165 0.0009
    1-166 0.0011
    1-167 0.0019
    1-168 0.0021
    1-169 0.0020
    1-170 0.0015
    1-171 0.0005
    1-172 0.017
    1-173 0.0054
    1-174 0.0031
    1-175 0.0013
    1-176 0.0018
    1-177 0.0013
    1-178 0.0014
    1-179 0.016
    1-180 0.0041
    1-181 0.0024
    1-182 0.0016
    1-183 0.0013
    1-184 0.0019
    1-185 0.0017
    1-186 0.0014
    1-187 0.0053
    1-188 0.0016
    1-189 1.1
    1-190 0.047
    1-191 0.015
    1-192 41% inhibition
    (at 30 μM)
    1-193 8.7
    1-194 10.5
    1-195 6.0
    1-196 0.042
    1-197 0.289
    1-198 0.014
    1-199 0.031
    1-200 0.010
    1-201 0.306
    1-202 0.0082
    1-203 0.020
    1-204 0.034
  • TABLE 4-5
    Human mPGES-1
    enzyme inhibitory
    Example activity (μM)
    1-205 0.367
    1-206 0.014
    1-207 0.0043
    1-208 0.016
    1-209 0.059
    1-210 0.288
    1-211 0.063
    1-212 0.032
    1-213 0.088
    1-214 0.024
    1-215 0.452
    1-216 0.039
    1-217 0.126
    1-218 0.070
    1-219 0.041
    1-220 0.016
    1-221 0.079
    1-222 0.165
    1-223 0.007
    1-224 5.8
    1-225 4.2
    1-226 2.2
    1-227 0.050
    1-228 0.672
    1-229 0.532
    1-230 0.750
    1-231 0.045
    1-232 0.521
    1-233 0.848
    1-234 1.0
    1-235 0.070
    1-236 0.263
    1-237 1.3
    1-238 0.0074
    1-239 0.428
    1-240 0.428
    1-241 0.278
    1-242 0.082
    1-243 0.120
    1-244 0.021
    1-245 0.108
    1-246 0.307
    1-247 0.011
    1-248 0.016
    1-249 0.226
    1-250 0.012
    1-251 0.018
    1-252 0.511
    1-253 0.791
    1-254 0.030
    1-255 0.045
  • TABLE 4-6
    Human mPGES-1
    enzyme inhibitory
    Example activity (μM)
    1-256 0.098
    1-257 0.017
    1-258 1.9
    1-259 0.176
    1-260 0.147
    1-261 44% inhibition
    (at 30 μM)
    1-262 0.007
    1-263 0.702
    1-264 0.163
    1-265 0.056
    1-266 0.011
    1-267 0.150
    2-1 0.283
    2-2 21.3
    2-3 14.7
    2-4 0.066
    2-5 0.101
    2-6 28% inhibition
    (at 30 μM)
    2-7 3.5
    2-8 1.9
    2-9 6.4
    2-10 0.073
    2-11 0.0060
    2-12 0.141
    2-13 23.1
    2-14 14.3
    2-15 16.4
    2-16 0.412
    2-17 0.039
    2-18 0.0080
    2-19 0.211
    2-20 0.052
    2-21 0.341
    2-22 0.219
    2-23 0.155
    2-24 2.4
    2-25 0.249
    2-26 2.7
    2-27 7.7
    2-28 3.7
    2-29 0.503
    2-30 43% inhibition
    (at 30 μM)
    2-31 0.031
    2-32 0.014
    2-33 0.102
    2-34 0.163
    2-35 0.017
    2-36 0.053
    2-37 0.041
    2-38 1.0
    2-39 0.450
  • TABLE 4-7
    Human mPGES-1
    enzyme inhibitory
    Example activity (μM)
    2-40 1.3
    2-41 0.429
    2-42 0.239
    2-43 0.570
    2-44 0.563
    2-45 0.012
    2-46 0.494
    2-47 0.295
    2-48 0.019
    2-49 0.014
    2-50 0.061
    2-51 0.090
    2-52 0.100
    2-53 0.011
    2-54 0.170
    2-55 0.010
    2-56 0.018
    2-57 0.025
    2-58 0.145
    2-59 0.095
    2-60 0.121
    2-61 0.092
    2-62 0.0093
    2-63 0.259
    2-64 0.012
    2-65 0.151
    2-66 0.016
    2-67 0.027
    2-68 0.672
    2-69 0.084
    2-70 0.158
    2-71 0.172
    2-72 0.283
    2-73 0.402
    2-74 0.424
    2-75 0.0037
    2-76 0.0058
    2-77 0.0037
    2-78 0.0068
    2-79 0.0037
    2-80 0.0016
    2-81 0.0027
    2-82 0.0017
    2-83 0.051
    2-84 0.017
    2-85 0.0016
    2-86 0.0022
    2-87 0.0018
    2-88 0.0020
    2-89 0.018
    2-90 2.1
  • TABLE 4-8
    Human mPGES-1
    enzyme inhibitory
    Example activity (μM)
    2-91 0.0016
    2-92 0.0044
    2-93 0.038
    2-94 0.0041
    2-95 0.0050
    2-96 0.021
    2-97 0.0089
    2-98 0.0037
    2-99 0.0017
    2-100 0.0056
    2-101 0.0065
    2-102 0.0059
    2-103 0.010
    2-104 0.018
    2-105 0.018
    2-106 0.0050
    2-107 0.0038
    2-108 0.0064
    2-109 0.0028
    2-110 0.0074
    2-111 0.0044
    2-112 0.0059
    2-113 0.0051
    2-114 0.0049
    2-115 0.0030
    2-116 0.0055
    2-117 0.0047
    2-118 0.0071
    2-119 0.0018
    2-120 0.0041
    2-121 0.014
    2-122 0.015
    2-123 0.012
    2-124 0.012
    2-125 0.0043
    2-126 0.0046
    2-127 0.0062
    2-128 0.013
    2-129 0.032
    2-130 0.035
    3-1 0.484
    3-2 0.148
    3-3 0.141
    3-4 0.202
    3-5 0.341
    3-6 0.056
    3-7 0.019
    3-8 0.0010
    3-9 0.776
    3-10 0.016
    3-11 0.093
  • TABLE 4-9
    Human mPGES-1
    enzyme inhibitory
    Example activity (μM)
    3-12 0.028
    3-13 0.034
    3-14 0.002
    3-15 0.0051
    3-16 0.0021
    3-17 0.0008
    3-18 0.0012
    3-19 24.6
    3-20 1.1
    3-21 0.611
    3-22 6.9
    3-23 0.041
    • Experimental Example 2 Evaluation of Action of mPGES-1 Inhibitor on Normal Intraocular Pressure of Macaca Fascicularis
  • This test was performed using male Macaca fascicularis. To eliminate interindividual difference and an influence of the difference in administration days, a crossover test was used for the evaluation as shown in Table 5.
  • TABLE 5
    Animal No. First course Second course Third course
    SX1M01 test article reference article vehicle
    10 mg/kg
    SX1M02 reference article vehicle test article
    30 mg/kg
    SX1M03 reference article vehicle test article
    30 mg/kg
    SX1M04 vehicle test article reference article
    30 mg/kg
    SX1M05 vehicle test article reference article
    30 mg/kg
  • To exclude the influence of the remaining test article, a 1-week washout period was set between tests. On the day of test, the monkeys were fed after the final measurement.
  • The test article (compound of Examples 2-98) was suspended in 0.5% methylcellulose (Wako Pure Chemical Industries, Ltd.), and administered by gavage by using a polypropylene syringe (sterilized disposable product, Nipro Corporation) and a stomach catheter (nelaton type A No. 9, Izumo health). The dose was set to 10 mg/kg/5 mL (N=1) or 30 mg/kg/5 mL (N=4) based on the body weight of each individual the day before the administration. To the vehicle group was administered the vehicle (0.5% methylcellulose (MC)) by a method similar to that for the test article. As a reference article, Xalatan (registered trade mark) ophthalmic solution 0.005% (Pfizer Inc., general name: latanoprost) was used. The reference article was administered by instillation of 20 μL thereof to one eye by using a micropipette. After instillation, the lacrimal part was lightly fixed by gently pressing the lower eyelid for about 15 seconds. The opposite eye was treated in the same manner. The intraocular pressure was measured immediately before administration, and 2, 4, 8, 12 and 24 hr after administration. Before measurement of the intraocular pressure, the animal was fixed on a monkey chair, and topically anesthetized by instillation of an ophthalmic surface anesthetic (Benoxyl (registered trade mark) ophthalmic solution 0.4%, Santen Pharmaceutical Co., Ltd., general name: oxybuprocaine hydrochloride). A lid rectactor (Handaya Co., Ltd.) was set, and the intraocular pressure of the both eyes was measured using a pneumatic applanation tonometer (Model30 Classic, Reichert Inc.).
  • To confirm disappearance of the test article, after an intraocular pressure measurement at 24 hr after the third course administration, blood samples (1 mL) were collected from the femoral vein under unanesthetized condition by using polypropylene syringe and 23 gauge injection needle (both sterilized disposable products) treated with heparin sodium, and the concentration of unaltered compound in the plasma containing the test article was measured.
  • An intraocular pressure difference (ΔmmHg; in first decimal place) from the value immediately before administration was determined for each measurement eye at each measurement time point, an average of the both eyes was calculated and taken as the evaluation data of the individual. The mean and standard deviation (in second decimal place) of the intraocular pressure difference was calculated for each group, and the test article administration group or reference article administration group was subjected to a homoscedasticity test (significance level 5%) based on F-test with the vehicle group. When the dispersion was equal, Student's t-test was performed and, when the dispersion was not equal, Aspin-Welch's t-test was performed. In addition, the maximum ocular hypotensive effect (ΔmmHg; maximum descent value from value immediately before administration, in first decimal place) was determined for each group, and the groups were compared in the same manner. The two-sided test was performed. It is a significant variation when a difference from the vehicle group was found at a 5% significance level and shown in FIG. 1 separately as 5% and 1%. Since the test article 10 mg/kg administration group contained only one animal, it was excluded from the statistical analysis.
  • The intraocular pressure of Macaca fascicularis used for this test before administration of a test article was 19.6±1.7 mmHg. After the measurement of intraocular pressure at 24 hr after the third course administration, the concentration of an unaltered test article in the plasma of the vehicle group and the reference article administration group was less than the lower detection limit. The results are shown in FIG. 1.
    • Experimental Example 3 Evaluation of Effect on Prostaglandin Composition in Guinea Pig Aqueous Humor
  • A test article was dissolved in saline containing 0.5% polysorbate80 (Fluka) and 0.003% ophthalmic solution (pH 7.0-8.0) was prepared. The test article was administered to Hartley male guinea pig by instillation of 20 μL thereof to one eye by using a micropipette. After instillation, the lacrimal part was lightly fixed by gently pressing the lower eyelid for about 15 seconds. The opposite eye was treated in the same manner. To the vehicle group was administered the medium (0.5% polysorbate-containing saline) by a method similar to that for the test article. After 23 hr from the instillation, Mydrin P (registered trade mark) 0.5% ophthalmic solution (Santen Pharmaceutical Co., Ltd., general name: tropicamide/phenylephrine hydrochloride) was dropwisely added by one drop to the both eyes of a guinea pig to cause mydriasis. The guinea pig was anesthetized with Escain (registered trade mark) inhalation anesthetics (Pfizer Inc., general name: isoflurane), the cornea of the both eyes was tapped with a 30 G injection needle, and the leaked aqueous humor (primary aqueous humor) was collected. One hour later (24 hr after instillation), the guinea pig was anesthetized again with isoflurane, and the secondary aqueous humor was collected in the same manner. The concentration of prostaglandins in the secondary aqueous humor obtained from each group (4 guinea pigs, 8 eyes) was measured by the LC/MS/MS system (Ultra high performance liquid chromatography: Nexera (registered trademark) manufactured by Shimadzu Corporation, mass spectrometer: AB SCIEX manufactured by QTRAP (registered trademark) 5500), and the concentration ratio of each prostaglandin concentration relative to the total of all prostaglandin concentrations was calculated. The results are shown in Table 6.
  • TABLE 6
    6-keto- PGD2
    Example PGE2 (%) PGF2α (%) PGF1α (%) (%) TXB2 (%)
    vehicle 80.8 7 6.8 4.7 0.7
    1-51 50.7 14 21.7 13.2 0.4
    1-81 60 9.8 15.7 13.2 1.3
    1-98 38 14.2 31.2 16.3 0.3
    1-109 29.5 14.1 37.5 18.9 0.1
    1-122 37.3 11.7 27.7 23 0.2
    1-128 36.2 13.9 29.7 19.3 0.8
    1-129 62.5 10.2 18.1 9.2 0
    1-130 73.6 8 11.2 6.2 1
    1-131 42.9 9.8 27.9 18.8 0.6
    1-135 56.1 12.7 19.4 10.9 0.9
    1-136 66.7 7.9 17.3 7.4 0.7
    1-137 49.5 11.3 24.8 14.1 0.3
    1-150 69 8.9 14 8 0.2
    1-169 28.7 13.5 40.3 17 0.5
    1-178 30 13 36.6 20.1 0.3
    1-184 57 10.3 21.2 10.7 0.8
    1-185 50 11 25.4 12.1 1.6
    2-98 37.8 14.8 27.3 20.1 0
    • Experimental Example 4 Evaluation of Action of mPGES-1 Inhibitor on Normal Intraocular Pressure of Macaca Fascicularis
  • This test is performed using male Macaca fascicularis. To eliminate interindividual difference and an influence of the difference in administration days, a crossover test is used for the evaluation as shown in Table 7.
  • TABLE 7
    Animal No. First course Second course Third course Fourth course
    SX1M01 vehicle test article test article + reference article
    reference article
    SX1M02 test article vehicle reference article test article +
    reference article
    SX1M03 reference article test article + test article vehicle
    reference article
    SX1M04 test article + test article vehicle reference article
    reference article
    SX1M05 vehicle reference article test article + test article
    reference article
    SX1M06 test article vehicle reference article test article +
    reference article
  • To exclude the influence of the remaining test article, a 1-week washout period is set between tests. On the day of test, the monkeys are fed after the final measurement.
  • A test article is dissolved in saline containing 0.5% polysorbate80 (Fluka) and 0.1% ophthalmic solution (pH 7.9-8.1) is prepared. To the vehicle group is administered the medium (0.5% polysorbate-containing saline) by a method similar to that for the test article. As a reference article, Xalatan (registered trademark) ophthalmic solution 0.005% (Pfizer Inc., general name: latanoprost) is used. The test article is administered by instillation of 30 μL thereof to one eye 5 times and 1 time of vehicle at 5-min intervals by using a micropipette (total 6 times instillation for each eye). Each of vehicle and reference article is administered 1 time and then vehicle is instilled 5 times (total 6 times instillation for each eye). In the test article+reference article combination group, the test article is instilled 5 times after instillation of the reference article (total 6 times instillation for each eye). After instillation at each time, the lacrimal part is lightly fixed by gently pressing the lower eyelid for about 15 seconds. The intraocular pressure is measured immediately before administration, and 2, 4, 8, 12 and 24 hr after administration. Before measurement of the intraocular pressure, the animal is fixed on a monkey chair, and topically anesthetized by instillation of an ophthalmic surface anesthetic (Benoxyl (registered trademark) ophthalmic solution 0.4%, Santen Pharmaceutical Co., Ltd., general name: oxybuprocaine hydrochloride). A lid rectactor (Handaya Co., Ltd.) is set, and the intraocular pressure of the both eyes is measured using a pneumatic applanation tonometer (Mode130 Classic, Reichert Inc.).
  • An intraocular pressure difference (ΔmmHg; in first decimal place) from the value immediately before administration is determined for each measurement eye at each measurement time point, an average of the both eyes is calculated and taken as the evaluation data of the individual. The mean and standard deviation (in second decimal place) of the intraocular pressure difference is calculated for each group, and the test article administration group or reference article administration group is subjected to a homoscedasticity test (significance level 5%) based on F-test with the vehicle group. When the dispersion is equal, Student's t-test is performed and, when the dispersion is not equal, Aspin-Welch's t-test is performed. In addition, the maximum ocular hypotensive effect (ΔmmHg; maximum descent value from value immediately before administration, in first decimal place) is determined for each group, and the groups are compared in the same manner. The two-sided test is performed. It is a significant variation when a difference from the vehicle group is found at a 5% significance level.
  • The Formulation Examples of the present invention include the following formulations. However, the present invention is not limited by such Formulation Examples.
    • Formulation Example 1 Production of Capsule
  • 1) compound of Example 1-86 30 mg
    2) microcrystalline cellulose 10 mg
    3) lactose 19 mg
    4) magnesium stearate  1 mg
  • 1), 2), 3) and 4) are mixed and filled in a gelatin capsule.
    • Formulation Example 2 Production of Tablet
  • 1) compound of Example 86 10 g
    2) lactose 50 g
    3) cornstarch 15 g
    4) carmellose calcium 44 g
    5) magnesium stearate  1 g
  • The total amount of 1), 2), 3) and 30 g of 4) are kneaded with water, vacuum dried and sieved. The sieved powder is mixed with 14 g of 4) and 1 g of 5), and the mixture is tableted by a tableting machine. In this way, 1000 tablets containing 10 mg of the compound of Example 1-86 per tablet are obtained. Formulation Example 3 (production of eye drop)
  • in 100 mL of eye drop
    1) compound of Example 1-86 100 mg
    2) polysorbate80 500 mg
    3) sodium chloride 900 mg
    4) sodium hydroxide q.s.
    5) sterilized purified water q.s.
  • The above components are aseptically blended to pH 7.9-8.1 to give an eye drop.
    • Formulation Example 4 Production of Eye Drop
  • in 100 mL of eye drop
    1) compound of Example 1-86 100 mg
    2) polysorbate80 100 mg
    3) sodium dihydrogen phosphate dehydrate 100 mg
    4) sodium chloride 900 mg
    5) benzalkonium chloride  5 mg
    6) sodium hydroxide q.s.
    7) sterilized purified water q.s.
  • The above components are aseptically blended to pH 7.9-8.1 to give an eye drop.
    • Formulation Example 5 Production of Eye Drop
  • in 100 mL of eye drop
    1) compound of Example 1-86 100 mg
    2) boric acid 700 mg
    3) borax q.s.
    4) sodium chloride 500 mg
    5) sodium edetate 0.05 mg 
    6) benzalkonium chloride 0.0005 mg  
    7) sterilized purified water q.s.
  • The above components are aseptically blended to pH 7.9-8.1 to give an eye drop.
    • INDUSTRIAL APPLICABILITY
  • Since the compound of the present invention and a pharmaceutically acceptable salt thereof have an mPGES-1 inhibitory activity, they can afford a medicament effective for the prophylaxis or treatment of pain, rheumatism, osteoarthritis, fever, Alzheimer's disease, multiple sclerosis, arteriosclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer including colorectal cancer and diseases for which suppression of PGE2 production is effective.
  • This application is based on a patent application No. 2014-031035 filed in Japan on Feb. 20, 2014, the contents of which are incorporated in full herein.

Claims (18)

1. A compound represented by the formula [I] or a pharmaceutically acceptable salt thereof:
Figure US20200087266A1-20200319-C00641
wherein
X is CH or N,
ring Cy is
the formula:
Figure US20200087266A1-20200319-C00642
or
the formula:
Figure US20200087266A1-20200319-C00643
{wherein R2 is
(1) halogen,
(2) C1-6 alkyl,
(3) cyano or
(4) haloC1-4 alkyl,
R2 is
(1) halogen,
(2) hydroxy,
(3) carboxy,
(4) C1-6 alkyl,
(5) C1-6 alkoxy,
(6) haloC1-4 alkoxy,
(7) haloC1-4 alkyl,
(8) C1-6 alkyl-carbonyl,
(9) —C(O)NRa1Ra2 (Ra1 and Ra2 are each independently hydrogen or C1-6 alkyl) or
(10) —(CnH2n)—Rb
(n is 1, 2, 3 or 4, —(CnH2n)— may be straight or branched chain, and
Rb is
(a) hydroxy,
(b) carboxy,
(c) C1-6 alkoxy,
(d) C1-6 alkyl-carbonyloxy,
(e) —C(O)NRb1Rb2 (Rb1 and Rb2 are each independently hydrogen or C1-6 alkyl),
(f) —OC(O)NRb3Rb4 (Rb3 and Rb4 are each independently hydrogen or C1-6 alkyl),
(g) —NRb5C(O) NRb6Rb7 (Rb5, Rb6 and Rb7 are each independently hydrogen or C1-6 alkyl),
(h) —NRb8Rb9 (Rb8 and Rb9 are each independently hydrogen, C1-6 alkyl or haloC1-4 alkyl),
(i) —NRb10S(O)2Rb11 (Rb10 and Rb11 are each independently hydrogen, C1-6 alkyl or C3-7 cycloalkyl),
(j) —NRb12C(O)ORb13 (Rb12 is hydrogen or C1-6 alkyl, and Rb13 is C1-6 alkyl),
(k) —NRb14C(O)Rb15 (Rb14 is hydrogen or C1-6 alkyl, and
Rb15 is
(i) C6-10 aryl,
(ii) C1-8 alkyl (said C1-8 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of hydroxy, haloC1-4 alkyl, C1-6 alkoxy and C6-10 aryl),
(iii) adamantyl or
(iv) C3-7 cycloalkyl (said C3-7 cycloalkyl is optionally substituted by 1, 2, 3 or 4 substituents selected from the group consisting of C1-6 alkyl, halogen, hydroxyl C1-6 alkyl and halo C1-4 alkyl, and/or optionally form a fused ring with a benzene ring), or
Rb14 and Rb15 optionally form a 4-, 5- or 6-membered lactam together with the nitrogen atom that Rb14 is bonded to and the carbon atom that Rb15 is bonded to (said lactam is optionally substituted by 1, 2 or 3 C1-6 alkyls, and/or optionally form a fused ring with a benzene ring),
(1) the formula:
Figure US20200087266A1-20200319-C00644
wherein m2 and m3 are each independently 1, 2 or 3, m4 is 0, 1, 2, 3 or 4, Rb16 is C1-6 alkyl or C1-6 alkoxy, and when m4 is 2, 3 or 4, each Rb16 is selected independently or
(m) the formula:
Figure US20200087266A1-20200319-C00645
wherein m5 and m6 are each independently 1, 2 or 3, and Rb17 is C1-6 alkyl or C1-6 alkoxy)
R3 is
(1) halogen,
(2) hydroxy,
(3) C1-6 alkyl or
(4) —ORc {Rc is C1-6 alkyl optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (a) to (f);
(a) halogen,
(b) hydroxy,
(c) C1-6 alkoxy,
(d) —C(O)NRc1Rc2 (Rc1 and Rc2 are each independently hydrogen or C1-6 alkyl),
(e) C6-10 aryl (said C6-10 aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
(i) halogen,
(ii) hydroxy,
(iii) C1-6 alkyl,
(iv) C1-6 alkoxy, and
(v) haloC1-4 alkyl), and
(f) 5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms (said heteroaryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
(i) halogen,
(ii) hydroxy,
(iii) C1-6 alkyl,
(iv) C1-6 alkoxy, and
(v) haloC1-4 alkyl)}, and
R4 is
(1) hydrogen,
(2) halogen,
(3) C1-6 alkyl or
(4) C1-6 alkoxy},
R5 is
(1) halogen,
(2) hydroxy,
(3) C1-6 alkylsulfanyl,
(4) C1-6 alkyl (said C1-6 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of halogen, C6-10 aryl and C1-6 alkoxy),
(5) C3-7 cycloalkyl,
(6) —ORd {Rd is
(a) C2-6 alkynyl,
(b) C3-7 cycloalkyl optionally substituted by 1, 2 or 3 C1-6 alkyls or
(c) C1-8 alkyl (said C1-8 alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (i) to (v);
(i) halogen,
(ii) C6-10 aryl,
(iii) C1-6 alkoxy,
(iv) C3-7 cycloalkyl (said C3-7 cycloalkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C1-6 alkyl and haloC1-4 alkyl), and
(v) 4-, 5- or 6-membered saturated heterocyclyl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms (said saturated heterocyclyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C1-6 alkyl and haloC1-4 alkyl))} or
(7) the formula:
Figure US20200087266A1-20200319-C00646
wherein Re is
(a) C1-6 alkyl,
(b) C3-7 cycloalkyl,
(c) 5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms, or
(d) C6-10 aryl (said C6-10 aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
(i) halogen,
(ii) C1-6 alkyl,
(iii) haloC1-4 alkyl,
(iv) C1-6 alkoxy, and
(v) haloC1-4 alkoxy), and
m1 is 0, 1, 2 or 3 and, when m 1 is 2 or 3, each R5 is selected independently,
excluding 4,6-bis-(2,5-dimethyl-phenyl)-1,3,5-triazin-2-ol.
2. The compound according to claim 1 or a pharmaceutically acceptable salt thereof, wherein ring Cy is the formula:
Figure US20200087266A1-20200319-C00647
wherein R1, R2 and R4 are as defined in claim 1.
3. The compound according to claim 1 or a pharmaceutically acceptable salt thereof, wherein ring Cy is the formula:
Figure US20200087266A1-20200319-C00648
wherein R1, R3 and R4 are as defined in claim 1.
4. The compound according to claim 1 or a pharmaceutically acceptable salt thereof, wherein X is CH.
5. The compound according to claim 1 or a pharmaceutically acceptable salt thereof, wherein X is N.
6. The compound according to claim 1 or a pharmaceutically acceptable salt thereof, wherein R1 is
(1) chloro,
(2) methyl,
(3) cyano or
(4) trifluoromethyl.
7. The compound according to claim 1 or a pharmaceutically acceptable salt thereof, wherein R4 is hydrogen.
8. The compound according to claim 1 or a pharmaceutically acceptable salt thereof, wherein R2 is
—(CnH2n)—Rb (n is 1 or 2, —(CnH2n)— may be straight or branched chain, and Rb is
(a) —C(O) NRb1Rb2,
(b) —NRb5C(O) NRb6Rb7,
(c) —NRb10S(O)2Rb11 or
(d) —NRb14C(O)Rb15
(Rb1, Rb2, Rb5, Rb6, Rb7, Rb10, Rb11, Rb14, and Rb15 are as defined in claim 1)).
9. The compound according to claim 8 or a pharmaceutically acceptable salt thereof, wherein R2 is —CH2—Rb (Rb is as defined in claim 8).
10. The compound according to claim 1 or a pharmaceutically acceptable salt thereof, wherein R3 is
(1) halogen,
(2) hydroxy,
(3) C1-6 alkyl or
(4) —ORc {Rc is C1-6 alkyl optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (a) to (f)
(a) halogen,
(b) hydroxy,
(c) C1-6 alkoxy,
(d) —C(O)NRc1Rc2 (Rc1 and Rc2 are each independently hydrogen or C1-6 alkyl),
(e) phenyl (said phenyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
(i) halogen,
(ii) hydroxy,
(iii) C1-6 alkyl,
(iv) C1-6 alkoxy, and
(v) haloC1-4 alkyl), and
(f) pyridyl (said pyridyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
(i) halogen,
(ii) hydroxy,
(iii) C1-6 alkyl,
(iv) C1-6 alkoxy, and
(v) haloC1-4 alkyl)}.
11. The compound according to claim 1 or a pharmaceutically acceptable salt thereof, wherein m 1 is 1, and
R5 is the formula:
Figure US20200087266A1-20200319-C00649
wherein Re is as defined in claim 1.
12. A compound selected from the following formulas:
Figure US20200087266A1-20200319-C00650
Figure US20200087266A1-20200319-C00651
Figure US20200087266A1-20200319-C00652
Figure US20200087266A1-20200319-C00653
Figure US20200087266A1-20200319-C00654
or a pharmaceutically acceptable salt thereof.
13. A pharmaceutical composition comprising the compound according to claim 1 or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
14.-16. (canceled)
17. A method of inhibiting mPGES-1, comprising administering a therapeutically effective amount of the compound according to claim 1 or a pharmaceutically acceptable salt thereof to a human in need thereof.
18. A method of treating or preventing pain, rheumatism, fever, osteoarthritis, arteriosclerosis, Alzheimer's disease, multiple sclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma or cancer, comprising administering a therapeutically effective amount of the compound according to claim 1 or a pharmaceutically acceptable salt thereof to a human in need thereof.
19. The method according to claim 18 for treating or preventing glaucoma or ocular hypertension, further comprising administering a therapeutically effective amount of one or more other therapeutic agents for glaucoma to the human in need thereof.
20.-21. (canceled)
US16/396,503 2014-02-20 2019-04-26 Triazine compounds and pharmaceutical use thereof Abandoned US20200087266A1 (en)

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Families Citing this family (13)

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Publication number Priority date Publication date Assignee Title
TW201613864A (en) 2014-02-20 2016-04-16 Takeda Pharmaceutical Novel compounds
TWI651310B (en) * 2014-02-20 2019-02-21 日商日本煙草產業股份有限公司 Triterpenoids and their medical use
JP6772141B2 (en) * 2014-12-24 2020-10-21 ナショナル・インスティチュート・オブ・バイオロジカル・サイエンシズ，ベイジン Necrosis inhibitor
WO2016139786A1 (en) * 2015-03-04 2016-09-09 株式会社島津製作所 Fluorescence derivatization reagent and amine analysis method
TWI704139B (en) 2015-08-17 2020-09-11 日商日本煙草產業股份有限公司 Hydroxytriazine compound and pharmaceutical use thereof
WO2017073709A1 (en) 2015-10-29 2017-05-04 あすか製薬株式会社 Pyrimidine derivative
GB201603311D0 (en) * 2016-02-25 2016-04-13 Jakobsson Per Johan New uses and methods
SG11201900558RA (en) * 2016-08-01 2019-02-27 Aptinyx Inc Spiro-lactam nmda receptor modulators and uses thereof
KR102641048B1 (en) * 2017-06-26 2024-02-27 메르크 파텐트 게엠베하 Method for preparing substituted nitrogen-containing heterocycles
EP4003340A4 (en) * 2019-07-22 2023-09-27 Angion Biomedica Corp. Ethynylheterocycles as rho-associated coiled-coil kinase (rock) inhibitors
US11400100B2 (en) * 2019-12-11 2022-08-02 Somerset Therapeutics, Llc. Effective benzalkonium chloride-free bimatoprost ophthalmic compositions
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WO2022168808A1 (en) 2021-02-02 2022-08-11 あすか製薬株式会社 Pyrimidine derivative

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1981003020A1 (en) * 1980-04-22 1981-10-29 Commw Scient Ind Res Org Triazine synthesis
DE19543730A1 (en) * 1995-11-23 1997-05-28 Ciba Geigy Ag Until resorcinyl-triazines
CN1246858A (en) * 1996-12-05 2000-03-08 安姆根有限公司 Substituted pyrimidine compounds and their use
AR038955A1 (en) * 1996-12-05 2005-02-02 Amgen Inc PIRIMIDINONE AND PIRIDONA SUBSTITUTED COMPOUNDS AND METHODS FOR USE
EP2138488A1 (en) * 2008-06-26 2009-12-30 sanofi-aventis 4-(pyridin-4-yl)-1H-[1,3,5]triazin-2-one derivatives as GSK3-beta inhibitors for the treatment of neurodegenerative diseases
CN101747282A (en) * 2008-12-10 2010-06-23 上海特化医药科技有限公司 Phenyl pyrimidone compounds, pharmaceutical compositions and preparation methods and uses thereof
US8952150B2 (en) * 2009-09-23 2015-02-10 Albert Einstein College Of Medicine Of Yeshiva University Prostaglandin transporter inhibitors and uses thereof
AR086254A1 (en) * 2011-05-26 2013-11-27 Lilly Co Eli USEFUL IMIDAZOL DERIVATIVES FOR THE TREATMENT OF ARTHRITIS
FR2983859B1 (en) * 2011-12-12 2014-01-17 Sanofi Sa 1,3,5-TRIAZINE-2-AMINE DERIVATIVES, THEIR PREPARATION AND THEIR DIAGNOSTIC AND THERAPEUTIC USE
KR101893112B1 (en) * 2012-01-06 2018-08-30 아지오스 파마슈티컬스 아이엔씨. Therapeutically active compounds and their methods of use
TWI568722B (en) * 2012-06-15 2017-02-01 葛蘭馬克製藥公司 Triazolone compounds as mpges-1 inhibitors
JP2014031035A (en) 2012-08-01 2014-02-20 Yamaha Motor Co Ltd Engine-assisted saddle-riding type vehicle
TWI651310B (en) * 2014-02-20 2019-02-21 日商日本煙草產業股份有限公司 Triterpenoids and their medical use
CN105586773A (en) * 2014-11-13 2016-05-18 东丽纤维研究所（中国）有限公司 Water-repellent anti-UV textile, production method and application thereof

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AU2015219920A1 (en) 2016-07-14
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CL2016002091A1 (en) 2017-01-20
JP2015172039A (en) 2015-10-01
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SA516371701B1 (en) 2019-01-21

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