US20190239863A1 - Non-invasive methods of detecting target molecules - Google Patents
Non-invasive methods of detecting target molecules Download PDFInfo
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- US20190239863A1 US20190239863A1 US16/244,752 US201916244752A US2019239863A1 US 20190239863 A1 US20190239863 A1 US 20190239863A1 US 201916244752 A US201916244752 A US 201916244752A US 2019239863 A1 US2019239863 A1 US 2019239863A1
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Definitions
- Embodiments of the present invention relate to non-invasive methods and compositions for collecting, detecting, measuring, and identifying target molecules.
- methods and compositions relate to target molecules in gastrointestinal lavage fluid (GLF) or feces.
- GLF gastrointestinal lavage fluid
- GI gastrointestinal
- pancreatic cancer e.g., pancreatic adenocarcinoma
- pancreatic adenocarcinoma is a malignant growth of the pancreas that mainly occurs in the cells of the pancreatic ducts. This disease is the ninth most common form of cancer, yet it is the fourth and fifth leading cause of cancer deaths in men and women, respectively. Cancer of the pancreas is almost always fatal, with a five-year survival rate that is less than 3%.
- pancreatic cancer The most common symptoms of pancreatic cancer include jaundice, abdominal pain, and weight loss, which, together with other presenting factors, are often nonspecific in nature. Thus, diagnosing pancreatic cancer at an early stage of tumor growth is often difficult and requires extensive diagnostic work-up, often times incidentally discovered during exploratory surgery. Endoscopic ultrasonography is an example non-surgical technique available for diagnosis of pancreatic cancer. However, reliable detection of small tumors, as well as differentiation of pancreatic cancer from focal pancreatitis, is difficult. The vast majority of patients with pancreatic cancer are presently diagnosed at a late stage when the tumor has already extended beyond the pancreas to invade surrounding organs and/or has metastasized extensively. Gold et al., Crit. Rev. Oncology/Hematology, 39:147-54 (2001), incorporated herein by reference in its entirety. Late detection of the disease is common with the majority of patients being diagnosed with advanced disease that often results in death; only a minority of patients are detected with early stage disease.
- the target molecules may be evaluated to determine whether they are useful as biomarkers associated with a particular characteristic, such as disease, predisposition to disease, positive response to a treatment regimen, or no response or negative response to a treatment regimen.
- biomarkers from the GI tract or associated organs/tissues may be used to determine whether an individual has any of the particular characteristics listed above.
- Embodiments of the present invention relate to non-invasive methods and compositions for collecting, detecting, measuring, and identifying target molecules.
- methods and compositions relate to target molecules in gastrointestinal lavage fluid (GLF) or feces.
- GLF gastrointestinal lavage fluid
- Some embodiments include a method for assessing the physiological state of a subject comprising: obtaining a gastrointestinal lavage fluid from the subject; and detecting a target molecule which originated from outside the gastrointestinal system in the gastrointestinal lavage fluid.
- Some embodiments include a method for assessing the physiological state of a subject comprising: obtaining a fecal sample from the subject; and detecting a target molecule which originated from outside the gastrointestinal system in the fecal sample.
- the gastrointestinal lavage fluid is obtained from the subject by partially purging the subject's gastrointestinal system.
- the gastrointestinal lavage fluid comprises fecal matter. In some embodiments, the fecal sample comprises a gastrointestinal lavage fluid.
- the target molecule comprises a polypeptide, antibody, bile acid, metabolite, or glycan.
- the target molecule comprises a biomarker.
- the biomarker is associated with a disease, a positive response to treatment, a partial response to treatment, a negative response to treatment, or no response to treatment.
- the target molecule is associated with presence of a cancer or predisposition to a cancer.
- the cancer is pancreatic cancer, colorectal cancer, liver cancer, or gastric cancer.
- the target molecule originated from an accessory digestive gland.
- the accessory digestive gland is salivary glands, pancreas, gallbladder, or liver.
- lavage fluid is administered orally.
- the lavage fluid comprises an ingredient selected from the group consisting of polyethylene glycol, magnesium sulfate, sodium sulfate, potassium sulfate, magnesium citrate, ascorbic acid, sodium picosulfate, and bisacodyl.
- the lavage fluid is selected from the group consisting of GOLYTELY, HALFLYTELY, NULYTELY, SUPREP, FLEET'S PHOSPHO-SODA, magnesium citrate, and their generic equivalents.
- Some embodiments include performing a colonoscopy on the subject.
- the subject is mammalian. In some embodiments, the subject is human.
- Some embodiments include a method for identifying a biomarker comprising: obtaining a test gastrointestinal lavage fluid from a plurality of test subjects having a condition or physiological state of interest and a control gastrointestinal lavage fluid from a plurality of control subjects who do not have said condition or physiological state; determining the level of at least 5 target molecules in the test gastrointestinal lavage fluid and the control gastrointestinal lavage fluid, and identifying a target molecule which is present at significantly different levels in the test gastrointestinal lavage fluid relative to the levels in the control gastrointestinal lavage fluid, thereby identifying a biomarker.
- the gastrointestinal lavage fluid comprises fecal matter.
- the target molecules are selected form the group consisting of polypeptides, bile acids, antibodies, metabolites, glycans, and a combination thereof.
- Some embodiments include determining the level of at least 10 target molecules in the test gastrointestinal lavage fluid and the control gastrointestinal lavage fluid. Some embodiments include determining the level of at least 20 target molecules in the test gastrointestinal lavage fluid and the control gastrointestinal lavage fluid. Some embodiments include determining the level of at least 30 target molecules in the test gastrointestinal lavage fluid and the control gastrointestinal lavage fluid. Some embodiments include determining the level of at least 50 target molecules in the test gastrointestinal lavage fluid and the control gastrointestinal lavage fluid. Some embodiments include determining the level of at least 100 target molecules in the test gastrointestinal lavage fluid and the control gastrointestinal lavage fluid.
- the biomarker is associated with a disease, a positive response to treatment, a partial response to treatment, a negative response to treatment or no response to treatment.
- the biomarker is associated with the presence of a cancer or predisposition to a cancer.
- the cancer is pancreatic cancer, liver cancer, or gastric cancer.
- At least one target molecule originated from an accessory digestive gland.
- the accessory digestive gland is salivary glands, pancreas, gallbladder, or liver.
- Some embodiments include administering a lavage fluid to the test subjects and the control subjects.
- the lavage fluid is administered orally.
- the lavage fluid comprises an ingredient selected from the group consisting of polyethylene glycol, magnesium sulfate, sodium sulfate, potassium sulfate, magnesium citrate, ascorbic acid, sodium picosulfate, and bisacodyl.
- the lavage fluid is selected from the group consisting of GOLYTELY, HALFLYTELY, NULYTELY, SUPREP, and FLEET'S PHOSPHO-SODA, magnesium citrate, and their generic equivalents.
- Some embodiments include performing a colonoscopy on the test subjects and control subjects.
- test subjects and control subjects are mammalian In some embodiments, the test subjects and control subjects are human.
- Some embodiments include a method for identifying a biomarker comprising: obtaining a test fecal sample from a plurality of test subjects having a condition of interest and a control fecal sample from a plurality of control subjects and; determining the level of at least 5 target molecules in the test fecal sample and the control fecal sample, identifying a target molecule which is present at significantly different levels in the test fecal sample relative to the levels in the control fecal sample, thereby identifying a biomarker.
- the target molecules are selected from the group consisting of polypeptides, bile acids, antibodies, metabolites, glycans, and a combination thereof.
- Some embodiments include determining the level of at least 10 target molecules in the test fecal sample and the control fecal sample. Some embodiments include determining the level of at least 20 target molecules in the fecal sample and the control fecal sample. Some embodiments include determining the level of at least 30 target molecules in the fecal sample and the control fecal sample. Some embodiments include determining the level of at least 50 target molecules in the fecal sample and the control fecal sample. Some embodiments include determining the level of at least 100 target molecules in the fecal sample and the control fecal sample.
- the biomarker is associated with a disease, a positive response to treatment, or a negative response to treatment.
- the biomarker is associated with the presence of a cancer or predisposition to a cancer.
- the cancer is pancreatic cancer, colorectal cancer, liver cancer, or gastric cancer.
- At least one target molecule originated from an accessory digestive gland.
- the accessory digestive gland is salivary glands, pancreas, gallbladder, or liver.
- test subjects and control subjects are mammalian In some embodiments, the test subjects and control subjects are human.
- kits for detecting a target molecule in a gastrointestinal lavage fluid comprising: a lavage fluid for oral administration to a subject; a vessel for collecting the gastrointestinal lavage fluid from the subject; and an agent for detecting a target molecule which originated from outside the gastrointestinal system.
- kits for detecting a target molecule in a fecal sample comprising: a lavage fluid for oral administration to a subject; a vessel for collecting the fecal sample from the subject; and an agent for detecting a target molecule which originated from outside the gastrointestinal system.
- Some embodiments include a protease inhibitor.
- the target molecule comprises a polypeptide, antibody, bile acid, metabolite, or glycan. In some embodiments, the target molecule comprises a biomarker. In some embodiments, the biomarker is associated with a disease, a positive response to treatment, or a negative response to treatment.
- the target molecule is associated with presence of a cancer or predisposition to a cancer.
- the cancer is pancreatic cancer, liver cancer, colorectal cancer, or gastric cancer.
- the target molecule originated from an accessory digestive gland.
- the accessory digestive gland is salivary glands, pancreas, gallbladder, or liver.
- the lavage fluid comprises an ingredient selected from the group consisting of polyethylene glycol, magnesium sulfate, sodium sulfate, potassium sulfate, magnesium citrate, ascorbic acid, sodium picosulfate, and bisacodyl.
- the lavage fluid is selected from the group consisting of GOLYTELY, HALFLYTELY, NULYTELY, SUPREP, FLEET'S PHOSPHO-SODA, magnesium citrate, and their generic equivalents.
- FIG. 2 depicts a graph of the relative abundance of compounds, including metabolites such as cholic acid, present in a fraction of a gastrointestinal lavage fluid. Data were acquired on a Waters Q-TOF mass spectrometer using input from an LC system, and using MassLynx software. The MS scanned over the mass range from m/z 100 m/z to 2000 every second.
- Embodiments of the present invention relate to non-invasive methods and compositions for collecting, detecting, measuring, and identifying target molecules.
- methods and compositions relate to target molecules in gastrointestinal lavage fluid (GLF) or feces.
- GLF gastrointestinal lavage fluid
- Gastrointestinal lavage is widely used as a lower gastrointestinal (GI) tract preparation for colonoscopy or colorectal surgery (see e.g., DiPalma J A. et al., (1984) Gastroenterology 86:856-60), incorporated herein by reference in its entirety).
- GI gastrointestinal
- Particular pathophysiologies of intestinal diseases have been investigated by measuring proteins in GLF (Evgenikos N, et al. (2000) Br J Surg 87:808-13; Brydon W G, Ferguson A. (1992) Lancet 340: 1381-2; Choudari C P, et al. (1993) Gastroenterology 104: 1064-71; Ferguson A, et al.
- GLF is preferred over feces as a sample for detecting and identifying biomarkers because GLF contains smaller amounts of substances that interfere with assays, and destruction of protein by digestive enzymes and bacterial proteases is less in GLF than a fecal sample because of its quick transit through the GI tract.
- a GLF can be produced by orally administering a lavage fluid to a subject that causes a large volume of fluid to pass through the intestinal tract
- the lavage fluid can contain a mixture of salts and other materials such as polyethylene glycol and bisacodyl.
- the lavage fluid causes an influx of liquid into the colon that causes a flushing out of solids.
- Lavage fluids are commonly used to cause clearing of the GI tract as is commonly used in preparation for a colonoscopy and other methods used to examine the GI tract. These liquids that are flushed out or remain in the largely cleared GI tract are useful to evaluate a variety of diseases due to the continuity of the mouth to the anus along the GI tract. Consequently, any and all organs, including the GI tract, which deposit fluids into the GI tract are candidates for the methods and compositions provided herein.
- the GI tract includes the upper GI tract and lower GI tract.
- the upper GI tract includes the oral or buccal cavity, esophagus, stomach and duodenum.
- the lower GI tract includes the jejunum, ileum and the large intestine and the anus.
- the large intestine includes the appendix, cecum, colon, and rectum.
- Organs and tissues associated with the GI tract include structures outside the GI tract.
- Examples of such structures include accessory digestive organs such as salivary glands, e.g., parotid salivary glands, submandibular salivary glands, and sublingual salivary glands, pancreas, e.g., exocrine pancreas, gallbladder, bile duct, and liver.
- More examples of structures associated with the GI tract and outside the GI tract include the pancreatic duct, biliary tree, and bile duct.
- a lavage fluid can be orally administered to a subject, the oral lavage fluid passes through the GI tract of the subject, and the resulting GLF is collected from the subject.
- the term “subject” can include an animal, such as a mammal, such as a human
- GLF provides a cleaner sampling of the GI tract than the examination of feces/stool samples. GLFs appear to mitigate variability related to food intake, type and digestive status.
- Some embodiments described herein include analysis of a GLF for detecting a target molecule or for screening, triage, disease detection, diagnosis, prognosis, response to treatment, selection of treatment and personalized medicine for diseases and pathological conditions of the gastrointestinal tract or associated organs/tissues. Some embodiments include analysis of a GLF sample for eliminating particular diseases and pathological conditions from the possible diseases or conditions from which a subject may be suffering. Some embodiments include analysis of a GLF for indicating the need for further testing for diagnosis. More embodiments include the analysis of GLF to establish a new disease diagnosis, further classifying a previous diagnosis, determining the sensitivity to potential treatment regimens, and/or evaluating the response to previous or ongoing treatment regimens.
- Some embodiments of the methods and compositions provided herein include obtaining a GLF from a subject.
- Methods of obtaining a GLF are well known in the art.
- medical and or diagnostic procedures such as sigmoidoscopy, colonoscopy, radiographic examination, preparation for patients undergoing bowel surgery, it is important that the bowels and colon be thoroughly purged and cleaned.
- Orthograde lavage can include orally administering a lavage composition to a subject comprising 4 L of a polyethylene glycol/electrolyte solution (U.S. Patent Application Publication No. 20070298008, incorporated by reference in its entirety). Some embodiments include antegrade lavage and retrograde lavage.
- oral lavage compositions include solutions of electrolytes, such as sodium, potassium and magnesium salts of sulfate, bicarbonate, chloride, phosphate or citrate. Some such compositions may also include polyethylene glycol, which can act as a non-absorbable osmotic agent. Generic compositions include polyethylene glycol with an electrolyte solution, optionally also including bisacodyl, or ascorbic acid, and compositions including sulfate salts such as sodium sulfate, magnesium sulfate, or potassium sulfate.
- an oral lavage fluid can include magnesium citrate.
- an oral lavage fluid can include sodium picosulfate.
- composition of an oral lavage solution comprising polyethylene glycol with an electrolyte solution
- GOLYTELY is formulated according to the following: polyethylene glycol 59 g, sodium sulfate 5.68 g, sodium bicarbonate 1.69 g, sodium chloride 1.46 g, potassium chloride 0.745 g and water to make up one liter (Davis et al. (1980) Gastroenterology 78:991-995, incorporated by reference in its entirety). Ingestion of GOLYTELY produces a voluminous, liquid stool with minimal changes in the subject's water and electrolyte balance.
- an oral lavage composition comprising polyethylene glycol with an electrolyte solution
- NULYTELY Braintree Labs. Inc.
- An example oral lavage composition comprising polyethylene glycol with an electrolyte solution and bisacodyl is HALFLYTELY (Braintree Labs. Inc.).
- An example oral lavage composition comprising sulfate salts, such as sodium sulfate, magnesium sulfate, or potassium sulfate is SUPREP (Braintree Labs. Inc.).
- An example composition of an oral lavage solution comprising polyethylene glycol with an electrolyte solution and ascorbic acid is MOVIPREP (Salix Pharmaceuticals, Inc.).
- Polyethylene glycol is effective as an oral lavage composition when large amounts of polyethylene glycol are administered in large volumes of a dilute salt solution. Usually about 250-400 g polyethylene glycol are administered to the subject in about 4 L of an electrolyte solution in water. Oral administration of polyethylene glycol can be used to produce a bowel movement over a period of time, e.g., overnight. The dose required will vary, but from about 10-100 g of polyethylene glycol in 8 oz. of water can be effective. A dose of from about 68-85 g of polyethylene glycol can be effective to produce an overnight bowel movement, without profuse diarrhea.
- a volume of a solution of polyethylene glycol in an isotonic fluid can be an effective amount of an osmotic laxative. Volumes from about 0.5 L to about 4 L can be effective. Preferably the effective volume is between about 1.5 L and about 2.5 L. Oral administration of 2 L of isotonic solution is effective.
- oral lavage compositions include hypertonic solutions of non-phosphate salts with an osmotic laxative agent such as polyethylene glycol (U.S. Pat. App. No. 20090258090, incorporated by reference in its entirety).
- osmotic laxative agent such as polyethylene glycol
- Mixtures of sulfate salts that omit phosphates, for example, effective amounts of one or more of the following sulfate salts Na 2 SO 4 , MgSO 4 , and K 2 SO 4 can be effective (e.g., SUPREP).
- Some embodiments include about 0.1 g to about 20.0 g Na 2 SO 4 , and from about 1.0 g to 10.0 g Na 2 SO 4 may be useful.
- Dosage amounts of MgSO 4 from about 0.01 g to about 40.0 g can be effective. Doses of from about 0.1 g to about 20.0 g Na 2 SO 4 may also be advantageously used, as well as dosages of 1.0 to 10.0 g. Dosage amounts of K 2 SO 4 from about 0.01 g to about 20.0 g can be effective to produce purgation, and doses of from about 0.1 g to about 10.0 g and from about 0.5 g to about 5.0 g K 2 SO 4 may also be useful. Addition of an osmotic laxative agent, such as polyethylene glycol (PEG) may improve the effectiveness of the above salt mixtures. Doses of PEG from about 1.0 g to about 100 g PEG are effective.
- PEG polyethylene glycol
- Doses from about 10.0 g to about 50 g of PEG are also effective, as is a dose of about 34 g.
- the above mixture of salts can be dissolved in a convenient volume of water. A volume of less than one liter of water can be well tolerated by most subjects.
- the mixture can be dissolved in any small volume of water, and volumes of between 100 and 500 ml are useful.
- the effective dose may be divided and administered to the patient in two or more administrations over an appropriate time period. Generally, administration of two doses of equal portions of the effective dose, separated by 6 to 24 hours produces satisfactory purgation.
- Some embodiments include cessation of normal oral intake during a defined period before and during administration of an oral lavage composition.
- lavage compositions include a laxative, such as bisacodyl.
- a laxative can be co-administered to a subject with a lavage composition.
- co-administration can include, for example, administration of a laxative up to several hours before administration of a lavage composition to a subject, administration of a laxative with the administration of a lavage composition to a subject, or administration of a laxative up to several hours after administration of a lavage composition to a subject.
- laxatives and their effective doses include Aloe, 250-1000 mg.; Bisacodyl, about 5-80 mg.; Casanthranol, 30-360 mg.; Cascara aromatic fluid extract, 2-24 ml.; Cascara sagrada bark, 300-4000 mg.; Cascada sagrada extract, 300-2000 mg.; Cascara sagrada fliuid extract, 0.5-5.0 ml.; Castor oil, 15-240 ml.; Danthron, 75-300 mg.; Dehydrocholic Acid, 250-2000 mg; Phenolphthalein, 30-1000 mg.; Sennosides A and B, 12-200 mg.; and Picosulfate, 1-100 mg.
- lavage compositions include aqueous solutions of concentrated phosphate salts.
- the aqueous phosphate salt concentrate produces an osmotic effect on the intra-luminal contents of the GI tract, evacuation of the bowel occurs with a large influx of water and electrolytes into the colon from the body.
- One exemplary composition comprises 480 g/L monobasic sodium phosphate and 180 g/L dibasic sodium phosphate in stabilized buffered aqueous solution (FLEET'S PHOSPHO-SODA, C. S. Fleet Co., Inc.). Subjects are typically required to take 2-3 oz doses of this composition, separated by a three to 12 hour interval for a total of 6 ounces (180 ml).
- GLF may be collected from a subject before, during, or after a medical or diagnostic procedure.
- a subject may collect GLF, for example, using a receptacle such as a toilet insert which captures the fluid.
- Enzyme inhibitors and denaturants may be used to preserve the quality of the GLF.
- the pH of the sample may be adjusted to help stabilize the samples.
- GLF samples may be further treated to remove some or all solids and/or bacteria, such as by centrifugation.
- the GI tract may not be fully purged by administration of an oral lavage composition. For example, a portion of a complete dose of an oral lavage composition required to fully purge the GI tract of a subject can be administered to the subject.
- a GLF can comprise fecal matter.
- fecal matter can comprise a GLF.
- target molecule includes any molecule that can be detected or measured or identified in a sample from the GI tract.
- samples include a GLF from a subject, and a fecal sample from a subject.
- target molecules include molecules such as peptides, polypeptides, proteins, mutant proteins, proteins generated from alternative splicing, modified proteins, such as post-translationally modified proteins e.g., glycosylated proteins, phosphorylated proteins, antibodies (e.g., autoantibodies, IgG, IgA, and IgM), antibody fragments, sugars, e.g., monosaccharides, disaccharides, oligosaccharides, and glycans, lipids, small molecules, e.g. metabolites, pharmaceutical compositions, metabolized pharmaceutical compositions, and pro-drugs. More examples of target molecules include bile salts and bile acids, e.g., cholic acid.
- Target molecules can originate in the GI tract and outside the GI tract, e.g., from organs and/or tissues associated with the GI tract, such as accessory digestive glands.
- cells including their fragments and their other biproducts e.g., red blood cells, white blood cells, and endothelial cells, organisms, e.g., bacteria, protozoans, and viruses and viral particles can be detected in a GLF or fecal samples.
- the target molecules may be any of the proteins or portions thereof listed in any of Tables 1-10 herein or a portion thereof.
- the portion of the proteins listed in any of Tables 1-10 can comprise at least 10, at least 15, at least 20, at least 25, at least 50, or more than 50 consecutive amino acids of any the proteins listed in Tables 1-10.
- the target molecules may comprise, consist essentially of, or consist of a polypeptide of one of SEQ ID NO.s: 01-804.
- a polypeptide consisting essentially of one of SEQ ID NO.s: 01-804 may include additional amino acids or substituents beyond those in SEQ ID NO.s: 01-804 where such additional amino acids or substituents do not prevent the polypeptide from being detectable.
- Target molecules also include biomarkers.
- biomarker includes any target molecule present in a GLF or fecal sample that is associated with a disease, predisposition to disease, positive response to a particular treatment regimen, no response to a particular treatment regimen, or negative response to a particular treatment regimen.
- a biomarker can be identified, measured and/or correlated with a diagnosis or prognosis of a disease.
- a target molecule is a component of a fluid of the subject selected from the group consisting of blood, saliva, gastric juice, hepatic secretion, bile, duodenal juice, and pancreatic juice.
- a target molecule is expressed in the upper gastrointestinal tract of the subject, or the lower gastrointestinal tract of the subject.
- a target molecule is expressed at a location in the subject selected from the group consisting of buccal cavity, esophagus, stomach, biliary tree, gallbladder, duodenum, jejunum, ileum, appendix, cecum, colon, rectum, and anal canal.
- a target molecule does not include a protein or other compound found in a GLF, for example, lactoferrin, eosinophil-derived neurotoxin, eosinophil cationic protein, bilirubin (Bil), alkaline phosphatase (ALP), aspartate aminotransferase, hemoglobin, or eosinophil peroxidase.
- a protein or other compound found in a GLF for example, lactoferrin, eosinophil-derived neurotoxin, eosinophil cationic protein, bilirubin (Bil), alkaline phosphatase (ALP), aspartate aminotransferase, hemoglobin, or eosinophil peroxidase.
- a target molecule does not include a protein found in feces, for example, heptaglobulin, hemopexin, a-2-macroglobulin, cadherin-17, calprotectin, carcinoembryogenic antigen, metalloproteinase-1 (TIMP-1), S100A12, K-ras, or p53.
- a protein found in feces for example, heptaglobulin, hemopexin, a-2-macroglobulin, cadherin-17, calprotectin, carcinoembryogenic antigen, metalloproteinase-1 (TIMP-1), S100A12, K-ras, or p53.
- a target molecule does not include a protein found in pancreatic juice, for example, anterior gradient-2 (AGR2), insulin-like growth factor binding protein-2, CEACAM6, MUC1, CA19-9, serine proteinase-2 (PRSS2) preproprotein, pancreatic lipase-related protein-1 (PLRP1), chymotrypsinogen B (CTRB), elastase 3B (ELA3B), tumor rejection antigen (pg96), azurocidin, hepatocarcinoma-intestine-pancreas/pancreatitis-associated-protein I (HIP/PAP-I), matrix metalloproteinase-9 (MMP-9), oncogene DJ1 (DJ-1), or alpha-1B-glycoprotein precursor (A1BG).
- AGR2 anterior gradient-2
- CEACAM6 insulin-like growth factor binding protein-2
- PRSS2 serine proteinase-2
- PRSS2 pancreatic lipase-related protein-1
- CTRB
- Some embodiments of the methods and compositions provided herein include characterizing a target molecule in a GLF or fecal sample. Characterizing a target molecule can include, for example, identifying a target molecule, detecting a target molecule, and/or quantifying a target molecule. Methods to identify, detect and quantify target molecule are well known in the art.
- Some embodiments include identifying, determining the presence or absence of a target molecule, and/or quantifying a target molecule, wherein the target molecule comprises a peptide, polypeptide, and/or protein.
- target molecules may be characterized by a variety of methods such as immunoassays, including radioimmunoassays, enzyme-linked immunoassays and two-antibody sandwich assays as described herein.
- immunoassay formats including competitive and non-competitive immunoassay formats, antigen capture assays and two-antibody sandwich assays also are useful (Self and Cook, (1996) Curr. Opin. Biotechnol. 7:60-65, incorporated by reference in its entirety).
- Some embodiments include one or more antigen capture assays.
- an antigen capture assay antibody is bound to a solid phase, and sample is added such that antigen, e.g., a target molecule in GLF or a fecal sample, is bound by the antibody. After unbound proteins are removed by washing, the amount of bound antigen can be quantitated, if desired, using, for example, a radioassay (Harlow and Lane, (1988) Antibodies A Laboratory Manual Cold Spring Harbor Laboratory: New York, incorporated by reference in its entirety) Immunoassays can be performed under conditions of antibody excess, or as antigen competitions, to quantitate the amount of antigen and, thus, determine a level of a target molecule in GLF or a fecal sample.
- Enzyme-linked immunosorbent assays can be useful in certain embodiments provided herein.
- An enzyme such as horseradish peroxidase (HRP), alkaline phosphatase (AP), ⁇ -galactosidase or urease can be linked, for example, to an anti-HMGB1 antibody or to a secondary antibody for use in a method of the invention.
- a horseradish-peroxidase detection system can be used, for example, with the chromogenic substrate tetramethylbenzidine (TMB), which yields a soluble product in the presence of hydrogen peroxide that is detectable at 450 nm.
- TMB chromogenic substrate tetramethylbenzidine
- enzyme-linked systems include, for example, the alkaline phosphatase detection system, which can be used with the chromogenic substrate p-nitrophenyl phosphate to yield a soluble product readily detectable at 405 nm.
- a ⁇ -galactosidase detection system can be used with the chromogenic substrate o-nitrophenyl- ⁇ -D-galactopyranoside (ONPG) to yield a soluble product detectable at 410 nm
- a urease detection system can be used with a substrate such as urea-bromocresol purple (Sigma Immunochemicals).
- Useful enzyme-linked primary and secondary antibodies can be obtained from a number of commercial sources such as Jackson Immuno-Research (West Grove, Pa.) as described further herein.
- a target molecule in GLF or a fecal sample can be detected and/or measured using chemiluminescent detection.
- specific antibodies to a particular target molecule are used to capture the target molecule present in the biological sample, e.g., GLF or a fecal sample and an antibody specific for the target molecule-specific antibodies and labeled with an chemiluminescent label is used to detect the target molecule present in the sample.
- Any chemiluminescent label and detection system can be used in the present methods.
- Chemiluminescent secondary antibodies can be obtained commercially from various sources such as Amersham. Methods of detecting chemiluminescent secondary antibodies are known in the art.
- Fluorescent detection also can be useful for detecting a target molecule in certain methods provided herein.
- Useful fluorochromes include, DAPI, fluorescein, Hoechst 33258, R-phycocyanin, B-phycoerythrin, R-phycoerythrin, rhodamine, Texas red and lissamine Fluorescein or rhodamine labeled antibodies, or fluorescein- or rhodamine-labeled secondary antibodies can be useful in the invention.
- Radioimmunoassays also can be useful in certain methods provided herein. Such assays are well known in the art. Radioimmunoassays can be performed, for example, with 125 I-labeled primary or secondary antibody (Harlow and Lane, supra, 1988).
- a signal from a detectable reagent can be analyzed, for example, using a spectrophotometer to detect color from a chromogenic substrate; a radiation counter to detect radiation, such as a gamma counter for detection of 125 I; or a fluorometer to detect fluorescence in the presence of light of a certain wavelength.
- a spectrophotometer to detect color from a chromogenic substrate
- a radiation counter to detect radiation, such as a gamma counter for detection of 125 I
- a fluorometer to detect fluorescence in the presence of light of a certain wavelength.
- quantitative analysis of the amount of a target molecule can be performed using a spectrophotometer such as an EMAX Microplate Reader (Molecular Devices; Menlo Park, Calif.) in accordance with the manufacturer's instructions.
- the assays of the invention can be automated or performed robotically, if desired, and that the signal from multiple samples can be detected simultaneously.
- capillary electrophoresis based immunoassays which can be automated if desired, may be used to detect and/or measure the target molecule.
- Immunoassays also can be used in conjunction with laser-induced fluorescence as described, for example, in Schmalzing and Nashabeh, Electrophoresis 18:2184-93 (1997), and Bao, J. Chromatogr. B. Biomed. Sci. 699:463-80 (1997), each incorporated by reference in its entirety.
- Liposome immunoassays such as flow-injection liposome immunoassays and liposome immunosensors, also can be used to detect target molecules or to determine a level of a target molecule according to certain methods provided herein (Rongen et al., (1997) J. Immunol. Methods 204:105-133, incorporated by reference in its entirety).
- Sandwich enzyme immunoassays also can be useful in certain embodiments.
- a first antibody is bound to a solid support, and the antigen is allowed to bind to the first antibody.
- the amount of a target molecule is quantitated by measuring the amount of a second antibody that binds to it.
- Quantitative Western blotting also can be used to detect a target molecule or to determine a level of target molecule in a method provided herein.
- Western blots can be quantitated by well known methods such as scanning densitometry.
- protein samples are electrophoresed on 10% SDS-PAGE Laemmli gels.
- Primary murine monoclonal antibodies, for example, against a target molecule are reacted with the blot, and antibody binding confirmed to be linear using a preliminary slot blot experiment.
- immunoassays including, for example, enzyme-linked immunosorbent assays, radioimmunoassays and quantitative western analysis, can be useful in some embodiments for detecting a target molecule or determining a level of a target molecule.
- Such assays typically rely on one or more antibodies.
- methods described herein can be used to readily distinguish proteins with alternative forms of post-translation modifications, e.g., phosphorylated proteins, and glycosylated proteins.
- Target molecules such as protein target molecules
- Proteins, polypeptides and peptides can be isolated by a variety of methods well known in the art, such as protein precipitation, chromatography (e.g., reverse phase chromatography, size exclusion chromatography, ion exchange chromatography, liquid chromatography), affinity capture, and differential extractions.
- chromatography e.g., reverse phase chromatography, size exclusion chromatography, ion exchange chromatography, liquid chromatography
- affinity capture e.g., affinity capture, and differential extractions.
- Isolated proteins can under go enzymatic digestion or chemical cleavage to yield polypeptide fragments and peptides. Such fragments can be identified and quantified.
- a particularly useful method for analysis of polypeptide/peptide fragments and other target molecules is mass spectrometry (U.S. Pat. App. No. 20100279382, incorporated by reference in its entirety).
- mass spectrometry-based quantitative proteomics methods have been developed that identify the proteins contained in each sample and determine the relative abundance of each identified protein across samples (Flory et al., Trends Biotechnol. 20:S23-29 (2002); Aebersold, J. Am. Soc. Mass Spectrom. 14:685-695 (2003); Aebersold, J. Infect.
- Samples with different isotopic signatures are then combined and analyzed, typically by multidimensional chromatography tandem mass spectrometry.
- the resulting collision induced dissociation (CID) spectra are then assigned to peptide sequences and the relative abundance of each detected protein in each sample is calculated based on the relative signal intensities for the differentially isotopically labeled peptides of identical sequence.
- More techniques for identifying and quantifying target molecules label-free quantitative proteomics methods include: (i) sample preparation including protein extraction, reduction, alkylation, and digestion; (ii) sample separation by liquid chromatography (LC or LC/LC) and analysis by MS/MS; (iii) data analysis including peptide/protein identification, quantification, and statistical analysis. Each sample can be separately prepared, then subjected to individual LC-MS/MS or LC/LC-MS/MS runs (Zhu W. et al., J. of Biomedicine and Biotech. (2010) Article ID 840518, 6 pages, incorporated by reference in its entirety).
- An example technique includes LC-MS in which the mass of a peptide coupled with its corresponding chromatographic elution time as peptide properties that uniquely define a peptide sequence, a method termed the accurate mass and time (AMT) tag approach.
- AMT accurate mass and time
- LC-FTICR Fourier transform ion cyclotron resonance
- these peptides can be relatively quantified by the signal intensity ratio of their corresponding peaks compared between MS runs (Tang, K., et al., (2004) J. Am. Soc. Mass Spectrom. 15:1416-1423; and Chelius, D. and Bondarenko, P. V. (2002) J. Proteome Res. 1: 317-323, incorporated by reference in their entireties).
- Statistics tools such as the Student's t-test can be used to analyse data from multiple LC-MS runs for each sample (Wiener, M. C., et al., (2004) Anal. Chem. 76:6085-6096, incorporated by reference in its entirety).
- the amplitudes of signal intensities from multiple LC-MS runs can be compared between two samples to detect peptides with statistically significant differences in abundance between samples.
- Mass analyzers with high mass accuracy, high sensitivity and high resolution include, ion trap, triple quadrupole, and time-of-flight, quadrupole time-of-flight mass spectrometeres and Fourier transform ion cyclotron mass analyzers (FT-ICR-MS).
- Mass spectrometers are typically equipped with matrix-assisted laser desorption (MALDI) or electrospray ionization (ESI) ion sources, although other methods of peptide ionization can also be used.
- MALDI matrix-assisted laser desorption
- ESI electrospray ionization
- ion trap MS In ion trap MS, analytes are ionized by ESI or MALDI and then put into an ion trap. Trapped ions can then be separately analyzed by MS upon selective release from the ion trap. Fragments can also be generated in the ion trap and analyzed. Sample molecules such as released polypeptide/peptide fragments can be analyzed, for example, by single stage mass spectrometry with a MALDI-TOF or ESI-TOF system. Methods of mass spectrometry analysis are well known to those skilled in the art (see, e.g., Yates, J. (1998) Mass Spect. 33:1-19; Kinter and Sherman, (2000) Protein Sequencing and Identification Using Tandem Mass. Spectrometry, John Wiley & Sons, New York; and Aebersold and Goodlett, (2001) Chem. Rev. 101:269-295, each incorporated by reference in its entirety).
- liquid chromatography ESI-MS/MS or automated LC-MS/MS which utilizes capillary reverse phase chromatography as the separation method, can be used (Yates et al., Methods Mol. Biol. 112:553-569 (1999), incorporated by reference in its entirety).
- Data dependent collision-induced dissociation (CID) with dynamic exclusion can also be used as the mass spectrometric method (Goodlett, et al., Anal. Chem. 72:1112-1118 (2000), incorporated by reference in its entirety).
- the resulting CID spectrum can be compared to databases for the determination of the identity of the isolated peptide.
- Methods for protein identification using single peptides have been described previously (Aebersold and Goodlett, Chem. Rev. 101:269-295 (2001); Yates, J. Mass Spec. 33:1-19 (1998), David N. et al., Electrophoresis, 20 3551-67 (1999), each incorporated by reference in its entirety).
- it is possible that one or a few peptide fragments can be used to identify a parent polypeptide from which the fragments were derived if the peptides provide a unique signature for the parent polypeptide.
- identification of a single peptide can be used to identify a parent glycopolypeptide from which the glycopeptide fragments were derived.
- methods that include MS can be used to characterize proteins, fragments thereof, as well as other types of target molecules described herein.
- Some embodiments can include enriching proteins and/or protein fractions of a GLF.
- Example methods can include protein precipitation, chromatography, such as reverse phase chromatography, size exclusion chromatography, ion exchange chromatography, liquid chromatography, as well as affinity capture, differential extraction methods and centrifugation. Proteins and/or protein fractions can be further examined using intact protein methods such as top-down proteomics or gel chromatography such as SDS-PAGE.
- Some embodiments include identifying, determining the presence or absence of a target molecule, and/or quantifying a target molecule, wherein the target molecule comprises a glycosylated protein and/or glycan.
- Glycosylated proteins and glycans can be analyzed by various methods well known in the art. Changes in glycosylation can be indicative of a disease or disease state.
- particular target molecules can include particular glycosylated proteins and/or glycans.
- a glycan may be a component of a glycoprotein, proteoglycan or other glycan containing compounds.
- Metabolites may be analyzed in a GLF or fecal sample using a variety of methods. For example, a GLF or fecal sample can be analyzed for metabolites using methods such as chromatography. Some components of the metabolome include bile acids and other small organic compounds. Metabolites can include peptides that are present in a GLF or fecal sample.
- the target molecules detected in GLF or a fecal sample can be evaluated to determine whether they are biomarkers associated with a particular condition, such as a disease, or physiological state.
- biomarkers can be indicative for a particular disease, predisposition to disease, prognosis, positive response to a particular treatment regimen, or negative response to a particular treatment regimen.
- the presence or absence, or level of a biomarker can be associated with a particular condition, such as a disease, or physiological state.
- the presence or absence, or level of a biomarker can be statistically correlated to the particular condition, such as a disease, or physiological state.
- a physiological state can include a disease.
- a biomarker can be correlated to a particular condition, such as disease, or physiological state by comparing the level of expression of a biomarker in a subject having a condition, such as a disease, or physiological state with the level of expression of the biomarker in a subject not having a condition or physiological state.
- the differential expression of a biomarker in a subject having a condition compared to the expression of a biomarker in a subject not having a condition is indicative of a condition or physiological state.
- “differential expression” refers to a difference in the level of expression of a biomarker in a subject having a condition, such as a disease, or physiological state and a subject not having the condition, such as a disease, or physiological state.
- the term “differential expression” can refer to the presence or absence of a biomarker in a subject having a condition, such as a disease, or physiological state compared with a subject not having a condition or physiological state.
- differential expression can refer to a difference in the level of expression of a biomarker in a subject having a condition, such as disease, or physiological state compared with the level of expression of a biomarker in a subject not having the condition, such as a disease, or physiological state.
- Differences in the level of a biomarker can be determined by measuring the amount or level of expression of a biomarker using methods provided herein.
- differential expression can be determined as the ratio of the levels of one or more biomarker products between reference subjects/populations having or not having a condition or physiological state, wherein the ratio is statistically significant.
- Differential expression between populations can be determined to be statistically significant as a function of p-value.
- the p-value is preferably less than 0.2.
- the biomarker is identified as being differentially expressed when the p-value is less than 0.15, 0.1, 0.05, 0.01, 0.005, 0.0001 etc.
- a biomarker product is differentially expressed if the ratio of the level of expression in a first sample as compared with a second sample is greater than or less than 1.0.
- a ratio of greater than 1.0 for example includes a ratio of greater than 1.1, 1.2, 1.5, 1.7, 2, 3, 4, 10, 20 and the like.
- a ratio of less than 1.0 for example, includes a ratio of less than 0.9, 0.8, 0.6, 0.4, 0.2, 0.1, 0.05 and the like.
- a biomarker can be differentially expressed if the ratio of the mean of the level of expression of a first population as compared with the mean level of expression of the second population is greater than or less than 1.0.
- a ratio of greater than 1.0 includes a ratio of greater than 1.1, 1.2, 1.5, 1.7, 2, 3, 4, 10, 20 and the like and a ratio less than 1.0, for example includes a ration of less than 0.9, 0.8, 0.6, 0.4, 0.2, 0.1, 0.05 and the like.
- a biomarker is differentially expressed if the ratio of its level of expression in a first sample as compared with the mean of the second population is greater than or less than 1.0 and includes for example, a ratio of greater than 1.1, 1.2, 1.5, 1.7, 2, 3, 4, 10, 20, or a ratio less than 1, for example 0.9, 0.8, 0.6, 0.4, 0.2, 0.1, 0.05.
- a biomarker can be identified by measuring the level of at least 1 target molecule in a test GLF or test fecal sample from at least one test subject having a condition or physiological state and a control GLF or control fecal sample from at least 1 control subject not having the condition or physiological state; comparing the level of the at least 1 target molecule in the test GLF or test fecal sample with the level of the at least 1 target molecule in the control GLF or control fecal sample, wherein a significant difference in the level of the at least 1 target identifies a biomarker.
- Some embodiments include measuring and comparing a plurality of target molecules in a test GLF or test fecal sample from plurality of test subjects having a condition or physiological state and a control GLF or control fecal sample from plurality of control subjects not having a condition or physiological state.
- at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100 target molecules can be measured and compared.
- a GLF or fecal sample can be obtained from at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100 test subjects.
- a GLF or fecal sample can be obtained from at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100 control subjects.
- a significant difference in the level of a target molecule in a test GLF or a test fecal sample compared to a control GLF or a control fecal sample can be a statistically significant.
- kits for detecting a target molecule in a GLF or fecal sample determining the presence or absence of a target molecule in a GLF or fecal sample, quantifying a target molecule in a GLF or fecal sample, or identifying a target molecule in a GLF or fecal sample.
- kits can include a lavage composition for oral administration to a subject.
- the lavage fluid can include an ingredient such as polyethylene glycol, magnesium sulfate, sodium sulfate, potassium sulfate, magnesium citrate, and bisacodyl.
- the lavage fluid can include polyethylene glycol with an electrolyte solution, optionally also including bisacodyl, or ascorbic acid (e.g., GOLYTELY, HALFLYTELY, NULYTELY, MOVIPREP).
- the lavage fluid can include phosphate salts (e.g. FLEET'S PHOSPHO-SODA).
- the lavage fluid can include sulfate salts such as sodium sulfate, magnesium sulfate, or potassium sulfate (e.g., SUPREP).
- the lavage fluid can include magnesium citrate.
- the lavage fluid can include sodium picosulfate.
- a kit can also include a vessel for collecting a GLF and/or fecal sample from a subject.
- a vessel for collecting a GLF can include an insert for a toilet which captures the GLF or fecal sample and the like.
- the vessel can include a material to stabilize and/or preserve a target molecule, such as one or more isolated protease inhibitors.
- the vessel can include an agent for detecting a target molecule, determining the presence or absence of a target molecule, quantifying a target molecule or identifying a target molecule.
- Some embodiments of the methods and compositions provided herein relate to the diagnosis, prognosis for a particular disease.
- Some embodiments include diseases and disorders related to the GI tract and organs associated therewith.
- Example diseases include cancers of the GI tract and organs associated therewith, e.g., gastric cancer, liver cancer, pancreatic cancer. More examples of diseases include pancreatitis, pancreatic adenocarcinoma, gastrointestinal neuroendocrine tumors, gastric adenocarcinoma, colon adenocarcinoma, hepatocellular carcinoma, cholangiocarcinoma, gallbladder adenoccarcinoma, ulcerative colitis, and Crohn's disease.
- Some diseases relate to an inflammatory bowel disease (IBD).
- IBD inflammatory bowel disease
- inflammatory bowel disease can refer to a broad class of diseases characterized by inflammation of at least part of the gastrointestinal tract. IBD symptoms may include inflammation of the intestine and resulting in abdominal cramping and persistent diarrhea. Inflammatory bowel diseases include ulcerative colitis (UC), Crohn's disease (CD), indeterminate colitis, chronic colitis, discontinuous or patchy disease, ileal inflammation, extracolonic inflammation, granulomatous inflammation in response to ruptured crypts, aphthous ulcers, transmural inflammation, microscopic colitis, diverticulitis and diversion colitis. More examples of diseases include celiac sprue, malabsorption disorders, and other conditions of digestive tract, liver, pancreas, and biliary tree.
- Some embodiments of the methods and compositions provided herein relate to determining the selection of a treatment (often referred to as personalized medicine), a subject's positive response to treatment, negative response to treatment, or lack of response to treatment. Some such embodiments include determining a patient's partial response to a treatment regimen. For example, the presence of a biomarker, absence of a biomarker, or level of a biomarker can be determined in a GLF or fecal sample from a subject at a first time point. At a second time point after treatment has begun and/or treatment has been completed, the presence of the biomarker, absence of the biomarker, or level of the biomarker can be determined in a GLF or fecal sample from the subject.
- the difference in the presence of the biomarker, absence of the biomarker, or level of the biomarker in the GLF or fecal sample at the second time point compared with the presence of the biomarker, absence of the biomarker, or level of the biomarker in the GLF or fecal sample from the first time point can be indicative of the subject's positive response to treatment, negative response to treatment, partial response to treatment, or lack of response to treatment.
- subjects can be given a treatment regimen and categorized as having a positive response, negative response, partial response, or no response.
- the presence, absence, or level of a target molecule in each group of subjects can be determined and those target molecules having a statistically significant association with each category of response can be identified.
- Some embodiments also include determining a future treatment regimen to be provided to a subject in view of determining the subject's positive response, negative response, partial response, or no response to a former or current treatment regimen. Accordingly, a former or current treatment regimen can be modified based on determinations made by the methods provided herein.
- More embodiments include methods for determining a subject's physiological status by evaluating a plurality of biomarkers. Some such methods include determining the presence, absence and/or levels of a plurality of biomarkers. The presence, absence and/or levels of a plurality of biomarkers can be correlated to the likelihood of the subject's physiological status, such as the subject's likelihood of developing a disease, and/or a subject's likely response to a treatment regimen to treat a particular disease. In some such methods, a subject's “clinical risk score” can be determined by correlating the presence, absence and/or levels of a plurality of biomarkers to determine the likelihood that a subject has a disease or will develop a disease (see, e.g., Soonmyung P. et al., (2004) New Eng. J. of Medicine 351:2817-2826; and Cho C. S. et al., (2008) J. Am. Coll. Surg. 206:281-291, incorporated by reference herein in their entireties).
- a sulfate-based GLF to support proteomic analysis was assessed.
- SUPREP was administered to three human subjects, and proteins in the resultant GLF were analyzed by mass spectroscopy.
- the GLF was collected from subjects as part of a colonoscopy procedure.
- a complete protease inhibitor tablet (ROCHE) was added and samples were spun at 1000 rpm for 30 minutes at 4° C. Supernatants were spun again at 14,000 ⁇ g for 30 min to pellet bacteria and debris. 1.8 ml of supernatant was precipitated with 6 volumes of acetone followed by extraction with an equal volume of chloroform followed by separation on a C-2 reverse phase SPE column (Sep-Pak, Waters). The column was washed with 3 column volumes each of 0.1% trifluoroacetic acid (TFA), 10%, 20%, and 30% acetonitrile (ACN) in 0.1% TFA, and eluted with 3 column volumes of 60% ACN in 0.1% TFA.
- TFA trifluoroacetic acid
- ACN acetonitrile
- Samples were dried by centrifugal lyophilization, resuspended in 100 ⁇ l of 50 mM ammonium bicarbonate/10 mM tris (2-carboxyethyl) phosphine and digested with 2 ⁇ l of 10 mM sequencing-grade trypsin (Promega, Madison, WI).
- Data were acquired on an LTQ-Orbitrap mass spectrometer using input from an LC system.
- the A solvent contained 3% of B and 0.2% formic acid in water.
- the B solvent contained 3% of A and 0.2% formic acid in acetonitrile.
- Solvents were HPLC grade from Fisher. For a 120 min run, the starting solvent was 5% B and remains for 7 min. The gradient was changed to 10% by 13 min, 40% by 83 min, 90% by 103 min, then reduced from 90% to 5% at 111 min. It was then re-equilibrated for the next injection. Three injections were performed for each sample for repeatability determination.
- MS was scanned (Orbitrap) over the mass range from 400 m/z to 2000 m/z every second while the LTQ (Trap) acquired up to 5 MSMS (peptide sequence) spectra in parallel. Data were acquired using the standard Thermo Xcalibur software. MS data (Orbitrap) was stable to 2-3 ppm and a background ion was used for mass drift assessment. MSMS data (LTQ) was measured to approximately 0.6 Da but the parent mass was acquired from the low ppm Orbitrap data. Peptides were eluted from a C18 LC column using triplicate injections to ensure reliability and repeatability of the data. A search file was created from the triplicate injections from each lavage preparation (patient sample) and converted into a MGF (Mascot Generic Format) file using a combination of Xcalibur and Mascot software packages.
- MGF Melt Generic Format
- a score threshold of “>40” was indicative of a p-value significance of ⁇ 0.05 as determined by the Mascot scoring system based on the search of this database with no enzyme specificity; a score of 40 is consistent with a p ⁇ 0.01.
- Standard Mascot scoring was used whereby only the highest score was added for each peptide detected, even if it was sampled during MS/MS multiple times. For all data included, scores were all >40 in at least one sample per protein line. For additional confidence, the numbers of significant peptides were also reported and a minimum criterion of at least 2 peptides was selected. Very few had less than 3 peptides.
- Table 1 lists examples of the most abundant proteins identified in GLF from three separate patients defined as patients 3, 4 and 6, presented in the format described above. As can be seen from Table 1, many proteins can be identified from GLF and a large number of these may be associated with the pancreas. Other proteins include DMBT1 (gi # 148539840) which may be associated with colon cancer and other cancers. Antibodies and putative glycosylated proteins were also identified.
- a polyethylene glycol based GLF to support proteomic analysis was assessed.
- a polyethylene glycol-based lavage composition was administered to two human subjects, and proteins in the resultant GLF were analyzed by mass spectrometry as described in Example 1. Removal of the polyethylene glycol was largely achieved by chloroform extraction of the lavage fluid. Many different proteins were identified in the GLFs from these subjects administered a polyethylene glycol-based lavage composition. Examples of the most abundant identified proteins identified, which are consistent with those observed in previous tables, are presented in Table 3.
- a PEG-based lavage composition was administered to a subject and the subject self-collected the resultant GLF into a collection container placed in the toilet immediately prior to colonoscopy.
- the proteome of the GLF was analyzed by MS as described herein. Many different proteins were identified in the self collected GLF sample. Examples of the most abundant identified proteins, and the corresponding Mascot scores and numbers of significant peptides for each protein are listed in Table 4. The more extensive protein list showed evidence of urinary contamination. A similar proteomic profile was observed for a sample collected subsequently during colonoscopy.
- the proteomes of GLFs resultant from the administration of sulfate-based lavage compositions and either collected during as part of a colonoscopy procedure or self-collected by a subject were compared.
- the Mascot scores and number of significant peptides for the most abundant proteins are summarized in Table 5. While there was a close correlation between the two proteomes observed, different isoforms were identified for at least two proteins. The selection of different isoforms may be a result of the collection of the sequence data during MS/MS and the search engine. There were fewer proteins detected in the self collected sample which was more dilute than that collected during the colonoscopy.
- a magnesium citrate based GLF was administered to a subject; the GLF was collected from subject as part of a colonoscopy procedure.
- the proteome of the GLF was analyzed by mass spectroscopy as described in Example 1. Many different proteins were identified in the GLF. Examples of the most abundant identified proteins are listed in Table 6. Many of the identified proteins were detected with different colonoscopy preparations suggesting that the proteome is not dependent on the bowel preparation used.
- SSL-7 affinity beads were used to isolate IgA-1 and IgA-2 specifically.
- 20 ⁇ l of SSL-7 agarose (Invitrogen, San Diego, Calif.) was added to 1 ml of sample and incubated overnight on a roller at 4° C. Tubes were spun at 1,000 ⁇ g for 2 minutes to pellet beads and supernatant discarded. Beads were washed 4 ⁇ with 1 ⁇ phosphate buffered saline and eluted with 20 ⁇ l of 100 mM glycine, pH 2.7 in a shaker for 1 hr at 600 rpm and 37° C.
- Eluted antibodies were diluted with 60 ⁇ l of 100 mM ammonium bicarbonate/10 mM tris (2-carboxyethyl) phosphine and digested with 2 ⁇ l of sequencing grade trypsin (Promega, Madison, Wis.). Mass spectrometry and database searches were performed as described above. The most abundant identified proteins present in GLF with affinity to SSL-7 and their corresponding Mascot scores are summarized in Table 7. As has been observed in prior examples, antibodies were present in the GLF and enrichment and analysis of these is possible using the affinity reagents, thus allowing the specific analysis of this subproteome in the GLF. The most abundant antibodies were IgAs. IgAs are consistently reported to be present in the intestinal tract.
- Protein L affinity beads were used to isolate antibodies containing kappa light chains. 20 ⁇ l of Protein L agarose (Santa Cruz Biotechnology, Santa Cruz, Calif.) was added to 1 ml of sample and incubated overnight on a roller at 4 C. Tubes were spun at 1,000 ⁇ g for 2 minutes to pellet beads and supernatant discarded. Beads were washed 4 times with 1 ⁇ phosphate buffered saline and eluted with 20 ⁇ l of 100 mM glycine, pH 2.7 in a shaker for 1 hr at 600 rpm and 37° C.
- Eluted antibodies were diluted with 60 ⁇ l of 100 mM Ammonium bicarbonate/10 mM tris (2-carboxyethyl) phosphine and digested with 2 ⁇ l of sequencing grade trypsin (Promega, Madison, Wis.).
- the most abundant identified proteins present in GLF with affinity to Protein L and their corresponding Mascot scores and numbers of significant peptides are summarized in Table 8.
- IgA and associated chains from antibodies were again detected.
- an IgM antibody (gi# 193806374) was also detected.
- a GLF sulfate-based lavage composition
- SOB Super Optimal Broth
- Pellets were lysed in a bead-beater in 8M urea and lysates were diluted to 2 M urea in 50 mM ammonium bicarbonate/10 mM tris (2-carboxyethyl) phosphine and digested with sequencing grade trypsin (Promega, Madison, Wis.). Data were acquired on the Orbitrap MS system using 120 mins runs as described earlier.
- a MGF search file was created and searched with the Mascot search engine (Matrix Science, UK) against the RefSeq database (http://www.ncbi.nlm nih.gov/RefSeq/) with the taxonomy specified as Eubacteria with a mass accuracy of 10 ppm for the parent ion (MS) and 0.6 Da for the fragment ions (MS/MS).
- MS parent ion
- MS/MS fragment ions
- the search files generated from the data acquired individually from 12 subjects were concatenated into a single search file and searched using the previously specified parameters for the Orbitrap data.
- Many proteins were analyzed in various GLF samples and proteins selected that (predominantly) had at least 3 unique significant peptides detected with thresholds of p ⁇ 0.05 (Mascot score approximately 41). Only 3 listed proteins (gi's 5031863, 6466801 and 115430223) had less than 3 significant peptides but these had Mascot scores of approximately 400, well above the 95% confidence level for protein identification. Proteins identified in this combined analysis are listed in Table 10 along with reported origins of particular proteins and reported associated cancers. Table 10 also lists SEQ ID NO.s. for identified peptides that had Mascot scores of 40 or greater for each unique identified protein. Many identified proteins have been reported to be present in pancreatic juice. References listed in Table 10 are provided in this application.
- GLF GLF was collected from a human subject. 1.8 mL GLF was added to 12 mL ice cold acetone and incubated for one hour to pellet the protein. The sample was centrifuged at 12,000 ⁇ g for 15 minutes and the acetone removed. After washing with ice cold acetone, the pellet was resuspended in 0.1% TFA and passed through a 5 mL, syringe style, SepPak C2 column Proteins were eluted with 60% acetonitrile/40% 0.1% TFA.
- the protein fraction was redissolved in 100 ⁇ L 50 mM ammonium bicarbonate and deglycosylated with 2 ⁇ L PNGaseF overnight at 37° C. in a shaker. After quenching with 1 mL 0.1% TFA, the glycans were collected as the flow through fraction from a 1 mL, syringe style, SepPak C18 column using a vacuum manifold.
- the dried glycans were labeled with 4-ABEE (ethyl 4-aminobenzoate) by reductive amination by adding 25 ⁇ L derivatizing solution (90:10 MeOH:HAc containing 35mM ABEE and 100 mM 2-PB) to the dried glycans, and incubating at 65° C. for 2 hours. Excess ABEE was removed by adding 1 mL ethyl ether, vortexing and discarding the ether. After a second ether extraction, the sample was briefly put in the SpeedVac to remove any residual ether. The labeled glycans were then run on the HPLC and eluted between 20-25% acetonitrile from an Agilent C8 reverse phase column.
- 4-ABEE ethyl 4-aminobenzoate
- FIG. 1 summarizes these results, and depicts a graph of the relative abundance of various glycoprotein derived glycan structures present in a fraction of a gastrointestinal lavage fluid. As shown in FIG.
- glycoprotein derived glycan structures include particular modifications that are associated with truncation of the chains. These modifications may be due to bacterial activity present in the GLF sample as it is known that bacteria can digest and consume glycans from proteins. However, such modifications can also be associated with disease, especially cancer where aberrant glycosylation has been linked to the disease.
- metabolites In this analysis, the ability of metabolites to be detected in samples obtained using a GLF was assessed.
- a magnesium citrate-based lavage composition was administered to human subjects, and the resultant GLF was analyzed for metabolites such as cholic acid and other bile salts.
- the resultant GLF was collected from the subjects as part of a colonoscopy procedure.
- 3 ml GLF was centrifuged at max speed for 20 minutes and the supernatant acidified with 0.1% TFA.
- the supernatant was applied to a C18 SPE column (Waters Sep-Pak), washed with 3 volumes of 0.1% TFA, and eluted with 50% ACN in 0.1% TFA.
- the elutant was dried by centrifugal lyophilization and re-dissolved in 500 ⁇ l 0.1% TFA.
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Abstract
Description
- This application is a continuation of U.S. patent application Ser. No. 14/344,399, filed Mar. 12, 2014; which is a national stage of International Patent Application No. PCT/US2011/051269, filed Sep. 12, 2011; the contents of each of the aforementioned applications are incorporated herein by reference in their entirety.
- Embodiments of the present invention relate to non-invasive methods and compositions for collecting, detecting, measuring, and identifying target molecules. In some embodiments, methods and compositions relate to target molecules in gastrointestinal lavage fluid (GLF) or feces.
- The present application is being filed along with a Sequence Listing in electronic format. The Sequence Listing is provided as a compliant ANSI text file, which is approximately 231 KB in size. The information in the electronic format of the Sequence Listing is incorporated herein by reference in its entirety.
- Disorders associated with the gastrointestinal (GI) and hepatobiliary tracts and the organs/tissues associated with the GI tract include cancers such as gastric cancer, esophageal cancer, liver cancer, and pancreatic cancer. Pancreatic cancer (e.g., pancreatic adenocarcinoma), in particular, is a malignant growth of the pancreas that mainly occurs in the cells of the pancreatic ducts. This disease is the ninth most common form of cancer, yet it is the fourth and fifth leading cause of cancer deaths in men and women, respectively. Cancer of the pancreas is almost always fatal, with a five-year survival rate that is less than 3%.
- The most common symptoms of pancreatic cancer include jaundice, abdominal pain, and weight loss, which, together with other presenting factors, are often nonspecific in nature. Thus, diagnosing pancreatic cancer at an early stage of tumor growth is often difficult and requires extensive diagnostic work-up, often times incidentally discovered during exploratory surgery. Endoscopic ultrasonography is an example non-surgical technique available for diagnosis of pancreatic cancer. However, reliable detection of small tumors, as well as differentiation of pancreatic cancer from focal pancreatitis, is difficult. The vast majority of patients with pancreatic cancer are presently diagnosed at a late stage when the tumor has already extended beyond the pancreas to invade surrounding organs and/or has metastasized extensively. Gold et al., Crit. Rev. Oncology/Hematology, 39:147-54 (2001), incorporated herein by reference in its entirety. Late detection of the disease is common with the majority of patients being diagnosed with advanced disease that often results in death; only a minority of patients are detected with early stage disease.
- Invasive techniques to diagnose disorders and diseases related to the GI tract are inconvenient and expose a subject to significant risk. Accordingly, there is a need for non-invasive methods and compositions for the detection and identification of target molecules from the GI tract or associated organs/tissues. In some embodiments, the target molecules may be evaluated to determine whether they are useful as biomarkers associated with a particular characteristic, such as disease, predisposition to disease, positive response to a treatment regimen, or no response or negative response to a treatment regimen. In addition, biomarkers from the GI tract or associated organs/tissues may be used to determine whether an individual has any of the particular characteristics listed above.
- Embodiments of the present invention relate to non-invasive methods and compositions for collecting, detecting, measuring, and identifying target molecules. In some embodiments, methods and compositions relate to target molecules in gastrointestinal lavage fluid (GLF) or feces.
- Some embodiments include a method for assessing the physiological state of a subject comprising: obtaining a gastrointestinal lavage fluid from the subject; and detecting a target molecule which originated from outside the gastrointestinal system in the gastrointestinal lavage fluid.
- Some embodiments include a method for assessing the physiological state of a subject comprising: obtaining a fecal sample from the subject; and detecting a target molecule which originated from outside the gastrointestinal system in the fecal sample.
- In some embodiments, the gastrointestinal lavage fluid is obtained from the subject by partially purging the subject's gastrointestinal system.
- In some embodiments, the gastrointestinal lavage fluid comprises fecal matter. In some embodiments, the fecal sample comprises a gastrointestinal lavage fluid.
- In some embodiments, the target molecule comprises a polypeptide, antibody, bile acid, metabolite, or glycan. In some embodiments, the target molecule comprises a biomarker. In some embodiments, the biomarker is associated with a disease, a positive response to treatment, a partial response to treatment, a negative response to treatment, or no response to treatment. In some embodiments, the target molecule is associated with presence of a cancer or predisposition to a cancer. In some embodiments, the cancer is pancreatic cancer, colorectal cancer, liver cancer, or gastric cancer. In some embodiments, the target molecule originated from an accessory digestive gland. In some embodiments, the accessory digestive gland is salivary glands, pancreas, gallbladder, or liver.
- Some embodiments include administering a lavage fluid to the subject. In some embodiments, lavage fluid is administered orally. In some embodiments, the lavage fluid comprises an ingredient selected from the group consisting of polyethylene glycol, magnesium sulfate, sodium sulfate, potassium sulfate, magnesium citrate, ascorbic acid, sodium picosulfate, and bisacodyl. In some embodiments, the lavage fluid is selected from the group consisting of GOLYTELY, HALFLYTELY, NULYTELY, SUPREP, FLEET'S PHOSPHO-SODA, magnesium citrate, and their generic equivalents.
- Some embodiments include performing a colonoscopy on the subject.
- In some embodiments, the subject is mammalian. In some embodiments, the subject is human.
- Some embodiments include a method for identifying a biomarker comprising: obtaining a test gastrointestinal lavage fluid from a plurality of test subjects having a condition or physiological state of interest and a control gastrointestinal lavage fluid from a plurality of control subjects who do not have said condition or physiological state; determining the level of at least 5 target molecules in the test gastrointestinal lavage fluid and the control gastrointestinal lavage fluid, and identifying a target molecule which is present at significantly different levels in the test gastrointestinal lavage fluid relative to the levels in the control gastrointestinal lavage fluid, thereby identifying a biomarker.
- In some embodiments, the gastrointestinal lavage fluid comprises fecal matter.
- In some embodiments, the target molecules are selected form the group consisting of polypeptides, bile acids, antibodies, metabolites, glycans, and a combination thereof.
- Some embodiments include determining the level of at least 10 target molecules in the test gastrointestinal lavage fluid and the control gastrointestinal lavage fluid. Some embodiments include determining the level of at least 20 target molecules in the test gastrointestinal lavage fluid and the control gastrointestinal lavage fluid. Some embodiments include determining the level of at least 30 target molecules in the test gastrointestinal lavage fluid and the control gastrointestinal lavage fluid. Some embodiments include determining the level of at least 50 target molecules in the test gastrointestinal lavage fluid and the control gastrointestinal lavage fluid. Some embodiments include determining the level of at least 100 target molecules in the test gastrointestinal lavage fluid and the control gastrointestinal lavage fluid.
- In some embodiments, the biomarker is associated with a disease, a positive response to treatment, a partial response to treatment, a negative response to treatment or no response to treatment.
- In some embodiments, the biomarker is associated with the presence of a cancer or predisposition to a cancer. In some embodiments, the cancer is pancreatic cancer, liver cancer, or gastric cancer.
- In some embodiments, at least one target molecule originated from an accessory digestive gland. In some embodiments, the accessory digestive gland is salivary glands, pancreas, gallbladder, or liver.
- Some embodiments include administering a lavage fluid to the test subjects and the control subjects. In some embodiments, the lavage fluid is administered orally. In some embodiments, the lavage fluid comprises an ingredient selected from the group consisting of polyethylene glycol, magnesium sulfate, sodium sulfate, potassium sulfate, magnesium citrate, ascorbic acid, sodium picosulfate, and bisacodyl. In some embodiments, the lavage fluid is selected from the group consisting of GOLYTELY, HALFLYTELY, NULYTELY, SUPREP, and FLEET'S PHOSPHO-SODA, magnesium citrate, and their generic equivalents.
- Some embodiments include performing a colonoscopy on the test subjects and control subjects.
- In some embodiments, the test subjects and control subjects are mammalian In some embodiments, the test subjects and control subjects are human.
- Some embodiments include a method for identifying a biomarker comprising: obtaining a test fecal sample from a plurality of test subjects having a condition of interest and a control fecal sample from a plurality of control subjects and; determining the level of at least 5 target molecules in the test fecal sample and the control fecal sample, identifying a target molecule which is present at significantly different levels in the test fecal sample relative to the levels in the control fecal sample, thereby identifying a biomarker.
- In some embodiments, the fecal sample comprises a gastrointestinal lavage fluid.
- In some embodiments, the target molecules are selected from the group consisting of polypeptides, bile acids, antibodies, metabolites, glycans, and a combination thereof.
- Some embodiments include determining the level of at least 10 target molecules in the test fecal sample and the control fecal sample. Some embodiments include determining the level of at least 20 target molecules in the fecal sample and the control fecal sample. Some embodiments include determining the level of at least 30 target molecules in the fecal sample and the control fecal sample. Some embodiments include determining the level of at least 50 target molecules in the fecal sample and the control fecal sample. Some embodiments include determining the level of at least 100 target molecules in the fecal sample and the control fecal sample.
- In some embodiments, the biomarker is associated with a disease, a positive response to treatment, or a negative response to treatment.
- In some embodiments, the biomarker is associated with the presence of a cancer or predisposition to a cancer. In some embodiments, the cancer is pancreatic cancer, colorectal cancer, liver cancer, or gastric cancer.
- In some embodiments, at least one target molecule originated from an accessory digestive gland. In some embodiments, the accessory digestive gland is salivary glands, pancreas, gallbladder, or liver.
- In some embodiments, the test subjects and control subjects are mammalian In some embodiments, the test subjects and control subjects are human.
- Some embodiments include a kit for detecting a target molecule in a gastrointestinal lavage fluid comprising: a lavage fluid for oral administration to a subject; a vessel for collecting the gastrointestinal lavage fluid from the subject; and an agent for detecting a target molecule which originated from outside the gastrointestinal system.
- Some embodiments include a kit for detecting a target molecule in a fecal sample comprising: a lavage fluid for oral administration to a subject; a vessel for collecting the fecal sample from the subject; and an agent for detecting a target molecule which originated from outside the gastrointestinal system.
- Some embodiments include a protease inhibitor.
- In some embodiments, the target molecule comprises a polypeptide, antibody, bile acid, metabolite, or glycan. In some embodiments, the target molecule comprises a biomarker. In some embodiments, the biomarker is associated with a disease, a positive response to treatment, or a negative response to treatment.
- In some embodiments, the target molecule is associated with presence of a cancer or predisposition to a cancer. In some embodiments, the cancer is pancreatic cancer, liver cancer, colorectal cancer, or gastric cancer.
- In some embodiments, the target molecule originated from an accessory digestive gland. In some embodiments, the accessory digestive gland is salivary glands, pancreas, gallbladder, or liver.
- In some embodiments, the lavage fluid comprises an ingredient selected from the group consisting of polyethylene glycol, magnesium sulfate, sodium sulfate, potassium sulfate, magnesium citrate, ascorbic acid, sodium picosulfate, and bisacodyl. In some embodiments, the lavage fluid is selected from the group consisting of GOLYTELY, HALFLYTELY, NULYTELY, SUPREP, FLEET'S PHOSPHO-SODA, magnesium citrate, and their generic equivalents.
-
FIG. 1 depicts a graph of the relative abundance of various glycoprotein derived glycan structures present in a fraction of a gastrointestinal lavage fluid. Derivatized glycans were eluted from a C18 reverse phase column on a Q-TOF MS at about 20-25% acetonitrile in 0.2% formic acid. The mass spectrometer was scanned in MS-only mode from m/z 150-2000 every second to acquire the derivatized glycan profile data. -
FIG. 2 depicts a graph of the relative abundance of compounds, including metabolites such as cholic acid, present in a fraction of a gastrointestinal lavage fluid. Data were acquired on a Waters Q-TOF mass spectrometer using input from an LC system, and using MassLynx software. The MS scanned over the mass range from m/z 100 m/z to 2000 every second. - Embodiments of the present invention relate to non-invasive methods and compositions for collecting, detecting, measuring, and identifying target molecules. In some embodiments, methods and compositions relate to target molecules in gastrointestinal lavage fluid (GLF) or feces.
- Gastrointestinal lavage is widely used as a lower gastrointestinal (GI) tract preparation for colonoscopy or colorectal surgery (see e.g., DiPalma J A. et al., (1984) Gastroenterology 86:856-60), incorporated herein by reference in its entirety). Particular pathophysiologies of intestinal diseases have been investigated by measuring proteins in GLF (Evgenikos N, et al. (2000) Br J Surg 87:808-13; Brydon W G, Ferguson A. (1992) Lancet 340: 1381-2; Choudari C P, et al. (1993) Gastroenterology 104: 1064-71; Ferguson A, et al. (1996) Gut 38:120-4; Handy L M, et al. (1996) Scand J Gastroenterol 31:700-5; and Stanley A J, et al. (1996) Gastroenterology 111:1679-82), each incorporated herein by reference in its entirety).
- Measurements of fecal proteins can be useful for investigating various pathophysiologies such as protein-losing enteropathy and mucosal inflammation. However, while feces may be used in some embodiments described herein, GLF is preferred over feces as a sample for detecting and identifying biomarkers because GLF contains smaller amounts of substances that interfere with assays, and destruction of protein by digestive enzymes and bacterial proteases is less in GLF than a fecal sample because of its quick transit through the GI tract. In addition, it is possible to estimate the rate of protein release from the mucosa, because the rate of fluid passage along the gut can be estimated.
- In some embodiments, a GLF can be produced by orally administering a lavage fluid to a subject that causes a large volume of fluid to pass through the intestinal tract, the lavage fluid can contain a mixture of salts and other materials such as polyethylene glycol and bisacodyl. The lavage fluid causes an influx of liquid into the colon that causes a flushing out of solids. Lavage fluids are commonly used to cause clearing of the GI tract as is commonly used in preparation for a colonoscopy and other methods used to examine the GI tract. These liquids that are flushed out or remain in the largely cleared GI tract are useful to evaluate a variety of diseases due to the continuity of the mouth to the anus along the GI tract. Consequently, any and all organs, including the GI tract, which deposit fluids into the GI tract are candidates for the methods and compositions provided herein.
- Some of the methods and compositions provided herein relate to the GI tract and organs/tissues associated with the GI tract including accessory digestive glands. As is well known in the art, the GI tract includes the upper GI tract and lower GI tract. The upper GI tract includes the oral or buccal cavity, esophagus, stomach and duodenum. The lower GI tract includes the jejunum, ileum and the large intestine and the anus. The large intestine includes the appendix, cecum, colon, and rectum.
- Organs and tissues associated with the GI tract include structures outside the GI tract. Examples of such structures include accessory digestive organs such as salivary glands, e.g., parotid salivary glands, submandibular salivary glands, and sublingual salivary glands, pancreas, e.g., exocrine pancreas, gallbladder, bile duct, and liver. More examples of structures associated with the GI tract and outside the GI tract include the pancreatic duct, biliary tree, and bile duct.
- Generally, a lavage fluid can be orally administered to a subject, the oral lavage fluid passes through the GI tract of the subject, and the resulting GLF is collected from the subject. As used herein, the term “subject” can include an animal, such as a mammal, such as a human As noted above, GLF provides a cleaner sampling of the GI tract than the examination of feces/stool samples. GLFs appear to mitigate variability related to food intake, type and digestive status.
- Some embodiments described herein include analysis of a GLF for detecting a target molecule or for screening, triage, disease detection, diagnosis, prognosis, response to treatment, selection of treatment and personalized medicine for diseases and pathological conditions of the gastrointestinal tract or associated organs/tissues. Some embodiments include analysis of a GLF sample for eliminating particular diseases and pathological conditions from the possible diseases or conditions from which a subject may be suffering. Some embodiments include analysis of a GLF for indicating the need for further testing for diagnosis. More embodiments include the analysis of GLF to establish a new disease diagnosis, further classifying a previous diagnosis, determining the sensitivity to potential treatment regimens, and/or evaluating the response to previous or ongoing treatment regimens.
- Some embodiments of the methods and compositions provided herein include obtaining a GLF from a subject. Methods of obtaining a GLF are well known in the art. For example, during medical and or diagnostic procedures such as sigmoidoscopy, colonoscopy, radiographic examination, preparation for patients undergoing bowel surgery, it is important that the bowels and colon be thoroughly purged and cleaned. In particular, it is essential that as much fecal matter as possible be removed from the colon to permit adequate visualization of the intestinal mucosa. This is important prior to, for example, diagnostic procedures such as flexible sigmoidoscopy or colonoscopy, diagnostic examinations widely performed to screen patients for diseases of the colon. In addition, it is important that the intestines be cleansed thoroughly in order to obtain satisfactory radiographs of the colon. The same condition also applies when the colon is preoperatively prepared for surgery, where removal of fecal waste materials is critically important for patient safety. To prepare the colon for endoscopic exam, current cleaning procedures include orthograde colonic lavage. Orthograde lavage can include orally administering a lavage composition to a subject comprising 4 L of a polyethylene glycol/electrolyte solution (U.S. Patent Application Publication No. 20070298008, incorporated by reference in its entirety). Some embodiments include antegrade lavage and retrograde lavage.
- Generally, oral lavage compositions include solutions of electrolytes, such as sodium, potassium and magnesium salts of sulfate, bicarbonate, chloride, phosphate or citrate. Some such compositions may also include polyethylene glycol, which can act as a non-absorbable osmotic agent. Generic compositions include polyethylene glycol with an electrolyte solution, optionally also including bisacodyl, or ascorbic acid, and compositions including sulfate salts such as sodium sulfate, magnesium sulfate, or potassium sulfate. In some embodiments, an oral lavage fluid can include magnesium citrate. In some embodiments, an oral lavage fluid can include sodium picosulfate. One example composition of an oral lavage solution comprising polyethylene glycol with an electrolyte solution is GOLYTELY (Braintree Labs. Inc.). GOLYTELY is formulated according to the following: polyethylene glycol 59 g, sodium sulfate 5.68 g, sodium bicarbonate 1.69 g, sodium chloride 1.46 g, potassium chloride 0.745 g and water to make up one liter (Davis et al. (1980) Gastroenterology 78:991-995, incorporated by reference in its entirety). Ingestion of GOLYTELY produces a voluminous, liquid stool with minimal changes in the subject's water and electrolyte balance. Another example of an oral lavage composition comprising polyethylene glycol with an electrolyte solution is NULYTELY (Braintree Labs. Inc.). An example oral lavage composition comprising polyethylene glycol with an electrolyte solution and bisacodyl is HALFLYTELY (Braintree Labs. Inc.). An example oral lavage composition comprising sulfate salts, such as sodium sulfate, magnesium sulfate, or potassium sulfate is SUPREP (Braintree Labs. Inc.). An example composition of an oral lavage solution comprising polyethylene glycol with an electrolyte solution and ascorbic acid is MOVIPREP (Salix Pharmaceuticals, Inc.).
- Polyethylene glycol is effective as an oral lavage composition when large amounts of polyethylene glycol are administered in large volumes of a dilute salt solution. Usually about 250-400 g polyethylene glycol are administered to the subject in about 4 L of an electrolyte solution in water. Oral administration of polyethylene glycol can be used to produce a bowel movement over a period of time, e.g., overnight. The dose required will vary, but from about 10-100 g of polyethylene glycol in 8 oz. of water can be effective. A dose of from about 68-85 g of polyethylene glycol can be effective to produce an overnight bowel movement, without profuse diarrhea. A volume of a solution of polyethylene glycol in an isotonic fluid can be an effective amount of an osmotic laxative. Volumes from about 0.5 L to about 4 L can be effective. Preferably the effective volume is between about 1.5 L and about 2.5 L. Oral administration of 2 L of isotonic solution is effective.
- More examples of oral lavage compositions include hypertonic solutions of non-phosphate salts with an osmotic laxative agent such as polyethylene glycol (U.S. Pat. App. No. 20090258090, incorporated by reference in its entirety). Mixtures of sulfate salts that omit phosphates, for example, effective amounts of one or more of the following sulfate salts Na2SO4, MgSO4, and K2SO4 can be effective (e.g., SUPREP). Some embodiments include about 0.1 g to about 20.0 g Na2SO4, and from about 1.0 g to 10.0 g Na2SO4 may be useful. Dosage amounts of MgSO4 from about 0.01 g to about 40.0 g can be effective. Doses of from about 0.1 g to about 20.0 g Na2SO4 may also be advantageously used, as well as dosages of 1.0 to 10.0 g. Dosage amounts of K2SO4 from about 0.01 g to about 20.0 g can be effective to produce purgation, and doses of from about 0.1 g to about 10.0 g and from about 0.5 g to about 5.0 g K2SO4 may also be useful. Addition of an osmotic laxative agent, such as polyethylene glycol (PEG) may improve the effectiveness of the above salt mixtures. Doses of PEG from about 1.0 g to about 100 g PEG are effective. Doses from about 10.0 g to about 50 g of PEG are also effective, as is a dose of about 34 g. For ease of administration, the above mixture of salts can be dissolved in a convenient volume of water. A volume of less than one liter of water can be well tolerated by most subjects. The mixture can be dissolved in any small volume of water, and volumes of between 100 and 500 ml are useful. The effective dose may be divided and administered to the patient in two or more administrations over an appropriate time period. Generally, administration of two doses of equal portions of the effective dose, separated by 6 to 24 hours produces satisfactory purgation. Some embodiments include cessation of normal oral intake during a defined period before and during administration of an oral lavage composition.
- Some lavage compositions include a laxative, such as bisacodyl. In some embodiments, a laxative can be co-administered to a subject with a lavage composition. As will be understood, such co-administration can include, for example, administration of a laxative up to several hours before administration of a lavage composition to a subject, administration of a laxative with the administration of a lavage composition to a subject, or administration of a laxative up to several hours after administration of a lavage composition to a subject. Examples of laxatives and their effective doses include Aloe, 250-1000 mg.; Bisacodyl, about 5-80 mg.; Casanthranol, 30-360 mg.; Cascara aromatic fluid extract, 2-24 ml.; Cascara sagrada bark, 300-4000 mg.; Cascada sagrada extract, 300-2000 mg.; Cascara sagrada fliuid extract, 0.5-5.0 ml.; Castor oil, 15-240 ml.; Danthron, 75-300 mg.; Dehydrocholic Acid, 250-2000 mg; Phenolphthalein, 30-1000 mg.; Sennosides A and B, 12-200 mg.; and Picosulfate, 1-100 mg.
- More examples of lavage compositions include aqueous solutions of concentrated phosphate salts. The aqueous phosphate salt concentrate produces an osmotic effect on the intra-luminal contents of the GI tract, evacuation of the bowel occurs with a large influx of water and electrolytes into the colon from the body. One exemplary composition comprises 480 g/L monobasic sodium phosphate and 180 g/L dibasic sodium phosphate in stabilized buffered aqueous solution (FLEET'S PHOSPHO-SODA, C. S. Fleet Co., Inc.). Subjects are typically required to take 2-3 oz doses of this composition, separated by a three to 12 hour interval for a total of 6 ounces (180 ml).
- GLF may be collected from a subject before, during, or after a medical or diagnostic procedure. In some embodiments, a subject may collect GLF, for example, using a receptacle such as a toilet insert which captures the fluid. Enzyme inhibitors and denaturants may be used to preserve the quality of the GLF. In some embodiments, the pH of the sample may be adjusted to help stabilize the samples. In some embodiments, GLF samples may be further treated to remove some or all solids and/or bacteria, such as by centrifugation. In some embodiments, the GI tract may not be fully purged by administration of an oral lavage composition. For example, a portion of a complete dose of an oral lavage composition required to fully purge the GI tract of a subject can be administered to the subject. In some embodiments, a GLF can comprise fecal matter. In more embodiments, fecal matter can comprise a GLF.
- Some embodiments described herein relate to methods of detecting target molecules in samples obtained from the GI tract or compositions useful for such detection. As used herein, “target molecule” includes any molecule that can be detected or measured or identified in a sample from the GI tract. Such samples include a GLF from a subject, and a fecal sample from a subject. Examples of target molecules include molecules such as peptides, polypeptides, proteins, mutant proteins, proteins generated from alternative splicing, modified proteins, such as post-translationally modified proteins e.g., glycosylated proteins, phosphorylated proteins, antibodies (e.g., autoantibodies, IgG, IgA, and IgM), antibody fragments, sugars, e.g., monosaccharides, disaccharides, oligosaccharides, and glycans, lipids, small molecules, e.g. metabolites, pharmaceutical compositions, metabolized pharmaceutical compositions, and pro-drugs. More examples of target molecules include bile salts and bile acids, e.g., cholic acid. More examples include chenodeoxycholic acid, glycocholic acid, taurocholic acid, deoxycholic acid, and lithocholic acid. Target molecules can originate in the GI tract and outside the GI tract, e.g., from organs and/or tissues associated with the GI tract, such as accessory digestive glands. In some embodiments, cells including their fragments and their other biproducts, e.g., red blood cells, white blood cells, and endothelial cells, organisms, e.g., bacteria, protozoans, and viruses and viral particles can be detected in a GLF or fecal samples. In some embodiments, the target molecules may be any of the proteins or portions thereof listed in any of Tables 1-10 herein or a portion thereof. In some embodiments, the portion of the proteins listed in any of Tables 1-10 can comprise at least 10, at least 15, at least 20, at least 25, at least 50, or more than 50 consecutive amino acids of any the proteins listed in Tables 1-10. In some embodiments, the target molecules may comprise, consist essentially of, or consist of a polypeptide of one of SEQ ID NO.s: 01-804. A polypeptide consisting essentially of one of SEQ ID NO.s: 01-804 may include additional amino acids or substituents beyond those in SEQ ID NO.s: 01-804 where such additional amino acids or substituents do not prevent the polypeptide from being detectable.
- Target molecules also include biomarkers. As used herein, the term “biomarker” includes any target molecule present in a GLF or fecal sample that is associated with a disease, predisposition to disease, positive response to a particular treatment regimen, no response to a particular treatment regimen, or negative response to a particular treatment regimen. In some embodiments, a biomarker can be identified, measured and/or correlated with a diagnosis or prognosis of a disease.
- In some embodiments, a target molecule is a component of a fluid of the subject selected from the group consisting of blood, saliva, gastric juice, hepatic secretion, bile, duodenal juice, and pancreatic juice. In some embodiments, a target molecule is expressed in the upper gastrointestinal tract of the subject, or the lower gastrointestinal tract of the subject. In some embodiments, a target molecule is expressed at a location in the subject selected from the group consisting of buccal cavity, esophagus, stomach, biliary tree, gallbladder, duodenum, jejunum, ileum, appendix, cecum, colon, rectum, and anal canal.
- In some embodiments, a target molecule does not include a protein or other compound found in a GLF, for example, lactoferrin, eosinophil-derived neurotoxin, eosinophil cationic protein, bilirubin (Bil), alkaline phosphatase (ALP), aspartate aminotransferase, hemoglobin, or eosinophil peroxidase. In some embodiments, a target molecule does not include a protein found in feces, for example, heptaglobulin, hemopexin, a-2-macroglobulin, cadherin-17, calprotectin, carcinoembryogenic antigen, metalloproteinase-1 (TIMP-1), S100A12, K-ras, or p53. In some embodiments, a target molecule does not include a protein found in pancreatic juice, for example, anterior gradient-2 (AGR2), insulin-like growth factor binding protein-2, CEACAM6, MUC1, CA19-9, serine proteinase-2 (PRSS2) preproprotein, pancreatic lipase-related protein-1 (PLRP1), chymotrypsinogen B (CTRB), elastase 3B (ELA3B), tumor rejection antigen (pg96), azurocidin, hepatocarcinoma-intestine-pancreas/pancreatitis-associated-protein I (HIP/PAP-I), matrix metalloproteinase-9 (MMP-9), oncogene DJ1 (DJ-1), or alpha-1B-glycoprotein precursor (A1BG).
- Some embodiments of the methods and compositions provided herein include characterizing a target molecule in a GLF or fecal sample. Characterizing a target molecule can include, for example, identifying a target molecule, detecting a target molecule, and/or quantifying a target molecule. Methods to identify, detect and quantify target molecule are well known in the art.
- Some embodiments include identifying, determining the presence or absence of a target molecule, and/or quantifying a target molecule, wherein the target molecule comprises a peptide, polypeptide, and/or protein. Such target molecules may be characterized by a variety of methods such as immunoassays, including radioimmunoassays, enzyme-linked immunoassays and two-antibody sandwich assays as described herein. A variety of immunoassay formats, including competitive and non-competitive immunoassay formats, antigen capture assays and two-antibody sandwich assays also are useful (Self and Cook, (1996) Curr. Opin. Biotechnol. 7:60-65, incorporated by reference in its entirety). Some embodiments include one or more antigen capture assays. In an antigen capture assay, antibody is bound to a solid phase, and sample is added such that antigen, e.g., a target molecule in GLF or a fecal sample, is bound by the antibody. After unbound proteins are removed by washing, the amount of bound antigen can be quantitated, if desired, using, for example, a radioassay (Harlow and Lane, (1988) Antibodies A Laboratory Manual Cold Spring Harbor Laboratory: New York, incorporated by reference in its entirety) Immunoassays can be performed under conditions of antibody excess, or as antigen competitions, to quantitate the amount of antigen and, thus, determine a level of a target molecule in GLF or a fecal sample.
- Enzyme-linked immunosorbent assays (ELISAs) can be useful in certain embodiments provided herein. An enzyme such as horseradish peroxidase (HRP), alkaline phosphatase (AP), β-galactosidase or urease can be linked, for example, to an anti-HMGB1 antibody or to a secondary antibody for use in a method of the invention. A horseradish-peroxidase detection system can be used, for example, with the chromogenic substrate tetramethylbenzidine (TMB), which yields a soluble product in the presence of hydrogen peroxide that is detectable at 450 nm. Other convenient enzyme-linked systems include, for example, the alkaline phosphatase detection system, which can be used with the chromogenic substrate p-nitrophenyl phosphate to yield a soluble product readily detectable at 405 nm. Similarly, a β-galactosidase detection system can be used with the chromogenic substrate o-nitrophenyl-β-D-galactopyranoside (ONPG) to yield a soluble product detectable at 410 nm, or a urease detection system can be used with a substrate such as urea-bromocresol purple (Sigma Immunochemicals). Useful enzyme-linked primary and secondary antibodies can be obtained from a number of commercial sources such as Jackson Immuno-Research (West Grove, Pa.) as described further herein.
- In certain embodiments, a target molecule in GLF or a fecal sample can be detected and/or measured using chemiluminescent detection. For example in certain embodiments, specific antibodies to a particular target molecule are used to capture the target molecule present in the biological sample, e.g., GLF or a fecal sample and an antibody specific for the target molecule-specific antibodies and labeled with an chemiluminescent label is used to detect the target molecule present in the sample. Any chemiluminescent label and detection system can be used in the present methods. Chemiluminescent secondary antibodies can be obtained commercially from various sources such as Amersham. Methods of detecting chemiluminescent secondary antibodies are known in the art.
- Fluorescent detection also can be useful for detecting a target molecule in certain methods provided herein. Useful fluorochromes include, DAPI, fluorescein, Hoechst 33258, R-phycocyanin, B-phycoerythrin, R-phycoerythrin, rhodamine, Texas red and lissamine Fluorescein or rhodamine labeled antibodies, or fluorescein- or rhodamine-labeled secondary antibodies can be useful in the invention.
- Radioimmunoassays (RIAs) also can be useful in certain methods provided herein. Such assays are well known in the art. Radioimmunoassays can be performed, for example, with 125I-labeled primary or secondary antibody (Harlow and Lane, supra, 1988).
- A signal from a detectable reagent can be analyzed, for example, using a spectrophotometer to detect color from a chromogenic substrate; a radiation counter to detect radiation, such as a gamma counter for detection of 125I; or a fluorometer to detect fluorescence in the presence of light of a certain wavelength. Where an enzyme-linked assay is used, quantitative analysis of the amount of a target molecule can be performed using a spectrophotometer such as an EMAX Microplate Reader (Molecular Devices; Menlo Park, Calif.) in accordance with the manufacturer's instructions. The assays of the invention can be automated or performed robotically, if desired, and that the signal from multiple samples can be detected simultaneously.
- In some embodiments, capillary electrophoresis based immunoassays (CEIA), which can be automated if desired, may be used to detect and/or measure the target molecule. Immunoassays also can be used in conjunction with laser-induced fluorescence as described, for example, in Schmalzing and Nashabeh, Electrophoresis 18:2184-93 (1997), and Bao, J. Chromatogr. B. Biomed. Sci. 699:463-80 (1997), each incorporated by reference in its entirety. Liposome immunoassays, such as flow-injection liposome immunoassays and liposome immunosensors, also can be used to detect target molecules or to determine a level of a target molecule according to certain methods provided herein (Rongen et al., (1997) J. Immunol. Methods 204:105-133, incorporated by reference in its entirety).
- Sandwich enzyme immunoassays also can be useful in certain embodiments. In a two-antibody sandwich assay, a first antibody is bound to a solid support, and the antigen is allowed to bind to the first antibody. The amount of a target molecule is quantitated by measuring the amount of a second antibody that binds to it.
- Quantitative Western blotting also can be used to detect a target molecule or to determine a level of target molecule in a method provided herein. Western blots can be quantitated by well known methods such as scanning densitometry. As an example, protein samples are electrophoresed on 10% SDS-PAGE Laemmli gels. Primary murine monoclonal antibodies, for example, against a target molecule are reacted with the blot, and antibody binding confirmed to be linear using a preliminary slot blot experiment. Goat anti-mouse horseradish peroxidase-coupled antibodies (BioRad) are used as the secondary antibody, and signal detection performed using chemiluminescence, for example, with the Renaissance chemiluminescence kit (New England Nuclear; Boston, Mass.) according to the manufacturer's instructions. Autoradiographs of the blots are analyzed using a scanning densitometer (Molecular Dynamics; Sunnyvale, Calif.) and normalized to a positive control. Values are reported, for example, as a ratio between the actual value to the positive control (densitometric index). Such methods are well known in the art as described, for example, in Parra et al., J. Vasc. Surg. 28:669-675 (1998), incorporated herein by reference in its entirety.
- As described herein, immunoassays including, for example, enzyme-linked immunosorbent assays, radioimmunoassays and quantitative western analysis, can be useful in some embodiments for detecting a target molecule or determining a level of a target molecule. Such assays typically rely on one or more antibodies. As would be understood by the skilled artisan, methods described herein can be used to readily distinguish proteins with alternative forms of post-translation modifications, e.g., phosphorylated proteins, and glycosylated proteins.
- Target molecules, such as protein target molecules, can be characterized by a variety of methods. Proteins, polypeptides and peptides can be isolated by a variety of methods well known in the art, such as protein precipitation, chromatography (e.g., reverse phase chromatography, size exclusion chromatography, ion exchange chromatography, liquid chromatography), affinity capture, and differential extractions.
- Isolated proteins can under go enzymatic digestion or chemical cleavage to yield polypeptide fragments and peptides. Such fragments can be identified and quantified. A particularly useful method for analysis of polypeptide/peptide fragments and other target molecules is mass spectrometry (U.S. Pat. App. No. 20100279382, incorporated by reference in its entirety). A number of mass spectrometry-based quantitative proteomics methods have been developed that identify the proteins contained in each sample and determine the relative abundance of each identified protein across samples (Flory et al., Trends Biotechnol. 20:S23-29 (2002); Aebersold, J. Am. Soc. Mass Spectrom. 14:685-695 (2003); Aebersold, J. Infect. Dis. 187 Suppl 2:S315-320 (2003); Patterson and Aebersold, Nat. Genet. 33 Suppl, 311-323 (2003); Aebersold and Mann, Nature 422:198-207 (2003); Aebersold, R. and Cravatt, Trends Biotechnol. 20:S1-2 (2002); Aebersold and Goodlett, Chem. Rev. 101, 269-295 (2001); Tao and Aebersold, Curr. Opin. Biotechnol. 14:110-118 (2003), each incorporated by reference in its entirety). Generally, the proteins in each sample are labeled to acquire an isotopic signature that identifies their sample of origin and provides the basis for accurate mass spectrometric quantification. Samples with different isotopic signatures are then combined and analyzed, typically by multidimensional chromatography tandem mass spectrometry. The resulting collision induced dissociation (CID) spectra are then assigned to peptide sequences and the relative abundance of each detected protein in each sample is calculated based on the relative signal intensities for the differentially isotopically labeled peptides of identical sequence.
- More techniques for identifying and quantifying target molecules label-free quantitative proteomics methods. Such methods include: (i) sample preparation including protein extraction, reduction, alkylation, and digestion; (ii) sample separation by liquid chromatography (LC or LC/LC) and analysis by MS/MS; (iii) data analysis including peptide/protein identification, quantification, and statistical analysis. Each sample can be separately prepared, then subjected to individual LC-MS/MS or LC/LC-MS/MS runs (Zhu W. et al., J. of Biomedicine and Biotech. (2010) Article ID 840518, 6 pages, incorporated by reference in its entirety). An example technique includes LC-MS in which the mass of a peptide coupled with its corresponding chromatographic elution time as peptide properties that uniquely define a peptide sequence, a method termed the accurate mass and time (AMT) tag approach. Using LC coupled with Fourier transform ion cyclotron resonance (LC-FTICR) MS to obtain the chromatographic and high mass accuracy information, peptide sequences can be identified by matching the AMT tags to previously acquired LC-MS/MS sequence information stored in a database. By taking advantage of the observed linear correlation between peak area of measured peptides and their abundance, these peptides can be relatively quantified by the signal intensity ratio of their corresponding peaks compared between MS runs (Tang, K., et al., (2004) J. Am. Soc. Mass Spectrom. 15:1416-1423; and Chelius, D. and Bondarenko, P. V. (2002) J. Proteome Res. 1: 317-323, incorporated by reference in their entireties). Statistics tools such as the Student's t-test can be used to analyse data from multiple LC-MS runs for each sample (Wiener, M. C., et al., (2004) Anal. Chem. 76:6085-6096, incorporated by reference in its entirety). At each point of acquisition time and m/z, the amplitudes of signal intensities from multiple LC-MS runs can be compared between two samples to detect peptides with statistically significant differences in abundance between samples.
- As will be understood, a variety of mass spectrometry systems can be employed in the methods for identifying and/or quantifying a polypeptide/peptide fragments. Mass analyzers with high mass accuracy, high sensitivity and high resolution include, ion trap, triple quadrupole, and time-of-flight, quadrupole time-of-flight mass spectrometeres and Fourier transform ion cyclotron mass analyzers (FT-ICR-MS). Mass spectrometers are typically equipped with matrix-assisted laser desorption (MALDI) or electrospray ionization (ESI) ion sources, although other methods of peptide ionization can also be used. In ion trap MS, analytes are ionized by ESI or MALDI and then put into an ion trap. Trapped ions can then be separately analyzed by MS upon selective release from the ion trap. Fragments can also be generated in the ion trap and analyzed. Sample molecules such as released polypeptide/peptide fragments can be analyzed, for example, by single stage mass spectrometry with a MALDI-TOF or ESI-TOF system. Methods of mass spectrometry analysis are well known to those skilled in the art (see, e.g., Yates, J. (1998) Mass Spect. 33:1-19; Kinter and Sherman, (2000) Protein Sequencing and Identification Using Tandem Mass. Spectrometry, John Wiley & Sons, New York; and Aebersold and Goodlett, (2001) Chem. Rev. 101:269-295, each incorporated by reference in its entirety).
- For high resolution polypeptide fragment separation, liquid chromatography ESI-MS/MS or automated LC-MS/MS, which utilizes capillary reverse phase chromatography as the separation method, can be used (Yates et al., Methods Mol. Biol. 112:553-569 (1999), incorporated by reference in its entirety). Data dependent collision-induced dissociation (CID) with dynamic exclusion can also be used as the mass spectrometric method (Goodlett, et al., Anal. Chem. 72:1112-1118 (2000), incorporated by reference in its entirety).
- Once a peptide is analyzed by MS/MS, the resulting CID spectrum can be compared to databases for the determination of the identity of the isolated peptide. Methods for protein identification using single peptides have been described previously (Aebersold and Goodlett, Chem. Rev. 101:269-295 (2001); Yates, J. Mass Spec. 33:1-19 (1998), David N. et al., Electrophoresis, 20 3551-67 (1999), each incorporated by reference in its entirety). In particular, it is possible that one or a few peptide fragments can be used to identify a parent polypeptide from which the fragments were derived if the peptides provide a unique signature for the parent polypeptide. Moreover, identification of a single peptide, alone or in combination with knowledge of a site of glycosylation, can be used to identify a parent glycopolypeptide from which the glycopeptide fragments were derived. As will be understood, methods that include MS can be used to characterize proteins, fragments thereof, as well as other types of target molecules described herein.
- Some embodiments can include enriching proteins and/or protein fractions of a GLF. Example methods can include protein precipitation, chromatography, such as reverse phase chromatography, size exclusion chromatography, ion exchange chromatography, liquid chromatography, as well as affinity capture, differential extraction methods and centrifugation. Proteins and/or protein fractions can be further examined using intact protein methods such as top-down proteomics or gel chromatography such as SDS-PAGE.
- Some embodiments include identifying, determining the presence or absence of a target molecule, and/or quantifying a target molecule, wherein the target molecule comprises a glycosylated protein and/or glycan. Glycosylated proteins and glycans can be analyzed by various methods well known in the art. Changes in glycosylation can be indicative of a disease or disease state. Thus, particular target molecules can include particular glycosylated proteins and/or glycans. As will be understood, a glycan may be a component of a glycoprotein, proteoglycan or other glycan containing compounds.
- Some embodiments include identifying, determining the presence or absence of a target molecule, and/or quantifying a target molecule, wherein the target molecule comprises a metabolite. Metabolites may be analyzed in a GLF or fecal sample using a variety of methods. For example, a GLF or fecal sample can be analyzed for metabolites using methods such as chromatography. Some components of the metabolome include bile acids and other small organic compounds. Metabolites can include peptides that are present in a GLF or fecal sample.
- In some embodiments, the target molecules detected in GLF or a fecal sample can be evaluated to determine whether they are biomarkers associated with a particular condition, such as a disease, or physiological state. Such biomarkers can be indicative for a particular disease, predisposition to disease, prognosis, positive response to a particular treatment regimen, or negative response to a particular treatment regimen. In some embodiments, the presence or absence, or level of a biomarker can be associated with a particular condition, such as a disease, or physiological state. In some embodiments, the presence or absence, or level of a biomarker can be statistically correlated to the particular condition, such as a disease, or physiological state. In some embodiments, a physiological state can include a disease. In some embodiments, a biomarker can be correlated to a particular condition, such as disease, or physiological state by comparing the level of expression of a biomarker in a subject having a condition, such as a disease, or physiological state with the level of expression of the biomarker in a subject not having a condition or physiological state.
- In some embodiments, the differential expression of a biomarker in a subject having a condition compared to the expression of a biomarker in a subject not having a condition is indicative of a condition or physiological state. As used herein, “differential expression” refers to a difference in the level of expression of a biomarker in a subject having a condition, such as a disease, or physiological state and a subject not having the condition, such as a disease, or physiological state. For example, the term “differential expression” can refer to the presence or absence of a biomarker in a subject having a condition, such as a disease, or physiological state compared with a subject not having a condition or physiological state. In some embodiments, differential expression can refer to a difference in the level of expression of a biomarker in a subject having a condition, such as disease, or physiological state compared with the level of expression of a biomarker in a subject not having the condition, such as a disease, or physiological state.
- Differences in the level of a biomarker can be determined by measuring the amount or level of expression of a biomarker using methods provided herein. In some embodiments, differential expression can be determined as the ratio of the levels of one or more biomarker products between reference subjects/populations having or not having a condition or physiological state, wherein the ratio is statistically significant. Differential expression between populations can be determined to be statistically significant as a function of p-value. When using p-value to determine statistical significance, a biomarker, the p-value is preferably less than 0.2. In another embodiment, the biomarker is identified as being differentially expressed when the p-value is less than 0.15, 0.1, 0.05, 0.01, 0.005, 0.0001 etc. When determining differential expression on the basis of the ratio, a biomarker product is differentially expressed if the ratio of the level of expression in a first sample as compared with a second sample is greater than or less than 1.0. For example, a ratio of greater than 1.0 for example includes a ratio of greater than 1.1, 1.2, 1.5, 1.7, 2, 3, 4, 10, 20 and the like. A ratio of less than 1.0, for example, includes a ratio of less than 0.9, 0.8, 0.6, 0.4, 0.2, 0.1, 0.05 and the like. In another embodiment, a biomarker can be differentially expressed if the ratio of the mean of the level of expression of a first population as compared with the mean level of expression of the second population is greater than or less than 1.0. For example, a ratio of greater than 1.0 includes a ratio of greater than 1.1, 1.2, 1.5, 1.7, 2, 3, 4, 10, 20 and the like and a ratio less than 1.0, for example includes a ration of less than 0.9, 0.8, 0.6, 0.4, 0.2, 0.1, 0.05 and the like. In another embodiment a biomarker is differentially expressed if the ratio of its level of expression in a first sample as compared with the mean of the second population is greater than or less than 1.0 and includes for example, a ratio of greater than 1.1, 1.2, 1.5, 1.7, 2, 3, 4, 10, 20, or a ratio less than 1, for example 0.9, 0.8, 0.6, 0.4, 0.2, 0.1, 0.05.
- In some embodiments, a biomarker can be identified by measuring the level of at least 1 target molecule in a test GLF or test fecal sample from at least one test subject having a condition or physiological state and a control GLF or control fecal sample from at least 1 control subject not having the condition or physiological state; comparing the level of the at least 1 target molecule in the test GLF or test fecal sample with the level of the at least 1 target molecule in the control GLF or control fecal sample, wherein a significant difference in the level of the at least 1 target identifies a biomarker. Some embodiments include measuring and comparing a plurality of target molecules in a test GLF or test fecal sample from plurality of test subjects having a condition or physiological state and a control GLF or control fecal sample from plurality of control subjects not having a condition or physiological state. In some embodiments, at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100 target molecules can be measured and compared. In some embodiments, a GLF or fecal sample can be obtained from at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100 test subjects. In some embodiments, a GLF or fecal sample can be obtained from at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100 control subjects. In some embodiments, a significant difference in the level of a target molecule in a test GLF or a test fecal sample compared to a control GLF or a control fecal sample can be a statistically significant.
- Some embodiments of the methods and compositions provided herein relate to kits for detecting a target molecule in a GLF or fecal sample, determining the presence or absence of a target molecule in a GLF or fecal sample, quantifying a target molecule in a GLF or fecal sample, or identifying a target molecule in a GLF or fecal sample. Some such kits can include a lavage composition for oral administration to a subject. In some embodiments, the lavage fluid can include an ingredient such as polyethylene glycol, magnesium sulfate, sodium sulfate, potassium sulfate, magnesium citrate, and bisacodyl. In some embodiments, the lavage fluid can include polyethylene glycol with an electrolyte solution, optionally also including bisacodyl, or ascorbic acid (e.g., GOLYTELY, HALFLYTELY, NULYTELY, MOVIPREP). In some embodiments, the lavage fluid can include phosphate salts (e.g. FLEET'S PHOSPHO-SODA). In some embodiments, the lavage fluid can include sulfate salts such as sodium sulfate, magnesium sulfate, or potassium sulfate (e.g., SUPREP). In some embodiments, the lavage fluid can include magnesium citrate. In some embodiments, the lavage fluid can include sodium picosulfate.
- In some embodiments, a kit can also include a vessel for collecting a GLF and/or fecal sample from a subject. A vessel for collecting a GLF can include an insert for a toilet which captures the GLF or fecal sample and the like. In some embodiments, the vessel can include a material to stabilize and/or preserve a target molecule, such as one or more isolated protease inhibitors. In some embodiments, the vessel can include an agent for detecting a target molecule, determining the presence or absence of a target molecule, quantifying a target molecule or identifying a target molecule.
- Some embodiments of the methods and compositions provided herein relate to the diagnosis, prognosis for a particular disease. Some embodiments include diseases and disorders related to the GI tract and organs associated therewith. Example diseases include cancers of the GI tract and organs associated therewith, e.g., gastric cancer, liver cancer, pancreatic cancer. More examples of diseases include pancreatitis, pancreatic adenocarcinoma, gastrointestinal neuroendocrine tumors, gastric adenocarcinoma, colon adenocarcinoma, hepatocellular carcinoma, cholangiocarcinoma, gallbladder adenoccarcinoma, ulcerative colitis, and Crohn's disease. Some diseases relate to an inflammatory bowel disease (IBD). As used herein, the term “inflammatory bowel disease” can refer to a broad class of diseases characterized by inflammation of at least part of the gastrointestinal tract. IBD symptoms may include inflammation of the intestine and resulting in abdominal cramping and persistent diarrhea. Inflammatory bowel diseases include ulcerative colitis (UC), Crohn's disease (CD), indeterminate colitis, chronic colitis, discontinuous or patchy disease, ileal inflammation, extracolonic inflammation, granulomatous inflammation in response to ruptured crypts, aphthous ulcers, transmural inflammation, microscopic colitis, diverticulitis and diversion colitis. More examples of diseases include celiac sprue, malabsorption disorders, and other conditions of digestive tract, liver, pancreas, and biliary tree.
- Some embodiments of the methods and compositions provided herein relate to determining the selection of a treatment (often referred to as personalized medicine), a subject's positive response to treatment, negative response to treatment, or lack of response to treatment. Some such embodiments include determining a patient's partial response to a treatment regimen. For example, the presence of a biomarker, absence of a biomarker, or level of a biomarker can be determined in a GLF or fecal sample from a subject at a first time point. At a second time point after treatment has begun and/or treatment has been completed, the presence of the biomarker, absence of the biomarker, or level of the biomarker can be determined in a GLF or fecal sample from the subject. The difference in the presence of the biomarker, absence of the biomarker, or level of the biomarker in the GLF or fecal sample at the second time point compared with the presence of the biomarker, absence of the biomarker, or level of the biomarker in the GLF or fecal sample from the first time point can be indicative of the subject's positive response to treatment, negative response to treatment, partial response to treatment, or lack of response to treatment. Alternatively, subjects can be given a treatment regimen and categorized as having a positive response, negative response, partial response, or no response. The presence, absence, or level of a target molecule in each group of subjects can be determined and those target molecules having a statistically significant association with each category of response can be identified. Some embodiments also include determining a future treatment regimen to be provided to a subject in view of determining the subject's positive response, negative response, partial response, or no response to a former or current treatment regimen. Accordingly, a former or current treatment regimen can be modified based on determinations made by the methods provided herein.
- More embodiments include methods for determining a subject's physiological status by evaluating a plurality of biomarkers. Some such methods include determining the presence, absence and/or levels of a plurality of biomarkers. The presence, absence and/or levels of a plurality of biomarkers can be correlated to the likelihood of the subject's physiological status, such as the subject's likelihood of developing a disease, and/or a subject's likely response to a treatment regimen to treat a particular disease. In some such methods, a subject's “clinical risk score” can be determined by correlating the presence, absence and/or levels of a plurality of biomarkers to determine the likelihood that a subject has a disease or will develop a disease (see, e.g., Soonmyung P. et al., (2004) New Eng. J. of Medicine 351:2817-2826; and Cho C. S. et al., (2008) J. Am. Coll. Surg. 206:281-291, incorporated by reference herein in their entireties).
- While the present invention has been described in some detail for purposes of clarity and understanding, one skilled in the art will appreciate that various changes in form and detail can be made without departing from the true scope of the invention.
- In this analysis, the ability of a sulfate-based GLF to support proteomic analysis was assessed. To identify target molecules in a GLF obtaining using a sulfate-based lavage composition, SUPREP was administered to three human subjects, and proteins in the resultant GLF were analyzed by mass spectroscopy. In this example, the GLF was collected from subjects as part of a colonoscopy procedure.
- Upon collection, a complete protease inhibitor tablet (ROCHE) was added and samples were spun at 1000 rpm for 30 minutes at 4° C. Supernatants were spun again at 14,000×g for 30 min to pellet bacteria and debris. 1.8 ml of supernatant was precipitated with 6 volumes of acetone followed by extraction with an equal volume of chloroform followed by separation on a C-2 reverse phase SPE column (Sep-Pak, Waters). The column was washed with 3 column volumes each of 0.1% trifluoroacetic acid (TFA), 10%, 20%, and 30% acetonitrile (ACN) in 0.1% TFA, and eluted with 3 column volumes of 60% ACN in 0.1% TFA. Samples were dried by centrifugal lyophilization, resuspended in 100 μl of 50 mM ammonium bicarbonate/10 mM tris (2-carboxyethyl) phosphine and digested with 2 μl of 10 mM sequencing-grade trypsin (Promega, Madison, WI).
- Data were acquired on an LTQ-Orbitrap mass spectrometer using input from an LC system. The A solvent contained 3% of B and 0.2% formic acid in water. The B solvent contained 3% of A and 0.2% formic acid in acetonitrile. Solvents were HPLC grade from Fisher. For a 120 min run, the starting solvent was 5% B and remains for 7 min. The gradient was changed to 10% by 13 min, 40% by 83 min, 90% by 103 min, then reduced from 90% to 5% at 111 min. It was then re-equilibrated for the next injection. Three injections were performed for each sample for repeatability determination.
- The MS was scanned (Orbitrap) over the mass range from 400 m/z to 2000 m/z every second while the LTQ (Trap) acquired up to 5 MSMS (peptide sequence) spectra in parallel. Data were acquired using the standard Thermo Xcalibur software. MS data (Orbitrap) was stable to 2-3 ppm and a background ion was used for mass drift assessment. MSMS data (LTQ) was measured to approximately 0.6 Da but the parent mass was acquired from the low ppm Orbitrap data. Peptides were eluted from a C18 LC column using triplicate injections to ensure reliability and repeatability of the data. A search file was created from the triplicate injections from each lavage preparation (patient sample) and converted into a MGF (Mascot Generic Format) file using a combination of Xcalibur and Mascot software packages.
- Database searching was done using the Mascot search engine (Matrix Science, UK) against the RefSeq database (http://www.ncbi.nlm nih gov/RefSeq/) with taxonomy specified as human (homo sapiens), a mass accuracy of 10 ppm for the parent ion (MS) and 0.6 Da for the fragment ions (MS/MS), and “no enzyme” selected. Searching without enzyme specificity was performed due to the presence of digestive enzymes in the sample that may modify or truncate peptides being examined The RefSeq database was supplemented by the addition of antibody sequences that are included in the SwissProt protein database, as these antibody sequences are not part of the standard RefSeq listing.
- Higher Mascot scores indicate better proteins hits and can be correlated to relative protein levels. A score threshold of “>40” was indicative of a p-value significance of <0.05 as determined by the Mascot scoring system based on the search of this database with no enzyme specificity; a score of 40 is consistent with a p<0.01. Standard Mascot scoring was used whereby only the highest score was added for each peptide detected, even if it was sampled during MS/MS multiple times. For all data included, scores were all >40 in at least one sample per protein line. For additional confidence, the numbers of significant peptides were also reported and a minimum criterion of at least 2 peptides was selected. Very few had less than 3 peptides. All significant peptides counted represented different sequences (individual peptides) from their respective proteins. The score and numbers of significant peptides are reported in the format x/y where x is the score and y the number of significant peptides. If a protein was not detected in a particular sample it is listed as “ND”. Proteins are reported as protein name and the “gi” number defined by the protein database of the NCBI has been provided. The sequences contained in each of the “gi” numbers in the NCBI database listed throughout the present application are incorporated herein by reference. Where a protein is named in its preprotein or other non-mature form, the mature form of the protein is equally implied including such changes as removal of signal sequences and the addition of post-translational modifications. In all cases, the protein has been named by its gene derived sequence to provide consistency.
- Table 1 lists examples of the most abundant proteins identified in GLF from three separate patients defined as patients 3, 4 and 6, presented in the format described above. As can be seen from Table 1, many proteins can be identified from GLF and a large number of these may be associated with the pancreas. Other proteins include DMBT1 (gi # 148539840) which may be associated with colon cancer and other cancers. Antibodies and putative glycosylated proteins were also identified.
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TABLE 1 Mascot score/ number of significant peptides NCBI gi # Protein Sample 3 Sample 4 Sample 6 10835000 pancreatic lipase precursor 2224/24 1238/13 2926/34 4506147 protease serine 2 preproprotein 665/7 46/0 1189/11 4506145 protease serine 1 preproprotein 239/2 64/0 1002/11 29725633 regenerating islet-derived 1 alpha 231/2 132/1 802/6 precursor 6679625 elastase 3B pancreatic preproprotein 852/12 1144/11 772/8 236460050 elastase 3A pancreatic preproprotein 1291/17 1306/14 769/7 118498350 chymotrypsin B2 945/10 244/2 724/6 15559207 elastase 2A preproprotein 752/10 952/8 593/6 54607080 pancreatic carboxypeptidase B1 702/9 84/1 499/4 preproprotein 50363217 serine proteinase inhibitor clade A 655/9 406/3 490/2 member 1 10280622 amylase pancreatic alpha-2B precursor ND ND 388/2 4505847 phospholipase A2 group IB 258/3 639/8 384/5 4502085 pancreatic amylase alpha 2A precursor 95/1 193/3 365/2 4502997 carboxypeptidase A1 precursor 454/4 88/1 349/5 62526043 chymotrypsin C preproprotein 696/9 440/3 343/4 148539840 deleted in malignant brain tumors 1 88/1 101/1 280/3 isoform a precursor (DMBT1) 31377806 polymeric immunoglobulin receptor 566/7 ND 279/3 precursor 41152086 serine (or cysteine) proteinase inhibitor ND 269/3 276/2 clade B (ovalbumin) member 6 148539842 deleted in malignant brain tumors 1 ND ND 275/3 isoform b precursor 4507149 superoxide dismutase 1 soluble ND 87/1 253/3 113584 RecName: Full = Ig alpha-1 chain C region 940/10 53/1 204/2 125145 RecName: Full = Ig kappa chain C region 659/9 106/1 180/1 98986445 carcinoembryonic antigen-related cell ND 219/3 135/0 adhesion molecule 5 preproprotein 218512088 RecName: Full = Ig alpha-2 chain C region 886/9 ND ND 119395750 keratin 1 ND 499/7 ND 55956899 keratin 9 ND 395/3 ND - In another experiment, SUPREP was administered to a subject according to the manufacturer's guidelines and the resultant GLF was self-collected by the subject into a collection container placed in the toilet immediately prior to colonoscopy. The proteome of the GLF was analyzed by MS as described above. The results showing the Mascot scores for the most abundant species present are summarized in Table 2. The results indicated some urinary contamination. A similar proteomic profile was observed for a sample collected subsequently during colonoscopy. Table 2 shows that many different proteins were identified in GLF collected by a subject. Identified proteins included DMBT1, pancreatic proteins and antibodies, consistent with data in Table 1.
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TABLE 2 Mascot score/ number of significant peptides NCBI gi # Protein Sample 25 148539842 deleted in malignant brain tumors 1 isoform b 1184/13 precursor 119395750 keratin 1 742/9 10835000 pancreatic lipase precursor 538/8 113584 RecName: Full = Ig alpha-1 chain C region 506/6 31377806 polymeric immunoglobulin receptor precursor 474/5 98986445 carcinoembryonic antigen-related cell adhesion 424/5 molecule 5 preproprotein 125817 RecName: Full = Ig kappa chain V-III region HAH; 382/5 Flags: Precursor 125797 RecName: Full = Ig kappa chain V-III region SIE 341/5 54607080 pancreatic carboxypeptidase B1 preproprotein 340/5 236460050 elastase 3A pancreatic preproprotein 328/4 125145 RecName: Full = Ig kappa chain C region 327/5 4502027 albumin preproprotein 319/3 33456 immunoglobulin M chain 239/3 118498350 chymotrypsin B2 238/3 125811 RecName: Full = Ig kappa chain V-III region VG; 237/3 Flags: Precursor 123843 RecName: Full = Ig heavy chain V-III region VH26; 219/3 Flags: Precursor 157266300 membrane alanine aminopeptidase precursor 213/3 563454 Ig heavy chain (VH4) V region (VDJ) 206/3 125788 RecName: Full = Ig kappa chain V-II region TEW 202/3 4506147 protease serine 2 preproprotein 177/3 125809 RecName: Full = Ig kappa chain V-III region CLL; 149/3 AltName: Full = Rheumatoid factor; Flags: Precursor - The foregoing analyses demonstrate that a large number of target molecules can be detected in samples obtained using a sulfate-based GLF.
- In this analysis, the ability of a polyethylene glycol based GLF to support proteomic analysis was assessed. To identify target molecules in a GLF obtaining using a polyethylene-based lavage composition, a polyethylene glycol-based lavage composition was administered to two human subjects, and proteins in the resultant GLF were analyzed by mass spectrometry as described in Example 1. Removal of the polyethylene glycol was largely achieved by chloroform extraction of the lavage fluid. Many different proteins were identified in the GLFs from these subjects administered a polyethylene glycol-based lavage composition. Examples of the most abundant identified proteins identified, which are consistent with those observed in previous tables, are presented in Table 3.
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TABLE 3 Mascot score/ number of significant peptides NCBI gi# Protein Sample 1 Sample 5 98986445 carcinoembryonic antigen-related cell adhesion 767/10 127/1 molecule 5 preproprotein 4502085 pancreatic amylase alpha 2A precursor 619/8 ND 1684927 immunoglobulin light chain 586/6 152/1 50363217 serine proteinase inhibitor clade A member 1 550/4 268/2 40254482 salivary amylase alpha 1A precursor 548/6 ND 298351713 RecName: Full = Ig lambda-1 chain C regions 501/6 139/1 4507725 transthyretin precursor 477/5 ND 236460050 elastase 3A pancreatic preproprotein 432/5 84/0 4502997 carboxypeptidase A1 precursor 412/5 106/1 113584 RecName: Full = Ig alpha-1 chain C region 404/4 214/3 4885165 cystatin A 352/4 66/1 40255013 carcinoembryonic antigen-related cell adhesion 349/3 ND molecule 6 (non-specific cross reacting antigen) 4506147 protease serine 2 preproprotein 326/5 434/6 125145 RecName: Full = Ig kappa chain C region 291/4 265/5 218512088 RecName: Full = Ig alpha-2 chain C region 272/3 ND 121039 RecName: Full = Ig gamma-1 chain C region 265/3 ND 54607080 pancreatic carboxypeptidase B1 preproprotein 263/2 ND - In another experiment, a PEG-based lavage composition was administered to a subject and the subject self-collected the resultant GLF into a collection container placed in the toilet immediately prior to colonoscopy. The proteome of the GLF was analyzed by MS as described herein. Many different proteins were identified in the self collected GLF sample. Examples of the most abundant identified proteins, and the corresponding Mascot scores and numbers of significant peptides for each protein are listed in Table 4. The more extensive protein list showed evidence of urinary contamination. A similar proteomic profile was observed for a sample collected subsequently during colonoscopy.
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TABLE 4 Mascot score/ number of significant peptides NCBI gi # Protein Sample 26 50363217 serine proteinase inhibitor clade A 406/3 member 1 4885165 cystatin A 294/3 4502027 albumin preproprotein 287/3 55956899 keratin 9 287/4 4506147 protease serine 2 preproprotein 227/3 4502085 pancreatic amylase alpha 2A precursor 217/3 - The proteomes of GLFs resultant from the administration of sulfate-based lavage compositions and either collected during as part of a colonoscopy procedure or self-collected by a subject were compared. The Mascot scores and number of significant peptides for the most abundant proteins are summarized in Table 5. While there was a close correlation between the two proteomes observed, different isoforms were identified for at least two proteins. The selection of different isoforms may be a result of the collection of the sequence data during MS/MS and the search engine. There were fewer proteins detected in the self collected sample which was more dilute than that collected during the colonoscopy.
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TABLE 5 Mascot score/ number of significant peptides Subject Colonoscopy collected NCBI gi # Protein collected sample sample 148539842 deleted in malignant brain tumors See isoform a 1184/13 1 isoform b precursor 148539840 deleted in malignant brain tumors 417/4 See isoform b 1 isoform a precursor 119395750 keratin 1 159/2 742/9 10835000 pancreatic lipase precursor 3719/37 538/8 113584 RecName: Full = Ig alpha-1 chain C 958/9 506/6 region 31377806 polymeric immunoglobulin 469/4 474/5 receptor precursor 98986445 carcinoembryonic antigen-related 74/1 424/5 cell adhesion molecule 5 preproprotein 125817 RecName: Full = Ig kappa chain V- ND 382/5 III region HAH; Flags: Precursor 125797 RecName: Full = Ig kappa chain V- ND 341/5 III region SIE 54607080 pancreatic carboxypeptidase B1 1389/17 340/5 preproprotein 236460050 elastase 3A pancreatic 2268/27 328/4 preproprotein 125145 RecName: Full = Ig kappa chain C 734/7 327/5 region 118498350 chymotrypsin B2 See chymotrypsin 238/3 B1 118498341 chymotrypsin B1 881/7 See chymotrypsin B2 62526043 chymotrypsin C preprotein 1002/10 ND 125811 RecName: Full = Ig kappa chain V- ND 237/3 III region VG; Flags: Precursor 1684927 immunoglobulin light chain 371/4 221/2 123843 RecName: Full = Ig heavy chain V- 181/2 219/3 III region VH26; Flags: Precursor 157266300 membrane alanine aminopeptidase 544/5 213/3 precursor 125788 RecName: Full = Ig kappa chain V- ND 202/3 II region TEW 4502085 pancreatic amylase alpha 2A 4258/49 ND precursor 10280622 amylase pancreatic alpha-2B 3916/45 ND precursor 6679625 elastase 3B pancreatic 1955/22 ND preproprotein 4506147 protease serine 2 preproprotein 1442/14 ND 4502997 carboxypeptidase A1 precursor 1168/14 ND 15559207 elastase 2A preproprotein 959/5 ND 217416390 carboxypeptidase A2 (pancreatic) 811/10 ND precursor 29725633 regenerating islet-derived 1 alpha 714/8 ND precursor 218512088 RecName: Full = Ig alpha-2 chain C 663/6 ND region 7669492 glyceraldehyde-3-phosphate 593/5 ND dehydrogenase 4506145 protease serine 1 preproprotein 551/6 ND 157266300 membrane alanine aminopeptidase 544/5 ND precursor 298351713 RecName: Full = Ig lambda-1 chain 322/4 ND C regions 119220569 zymogen granule membrane 304/3 ND glycoprotein 2 isoform 1 51593090 mucin 13 epithelial 293/3 ND transmembrane 125807 RecName: Full = Ig kappa chain V- 288/3 ND III region GOL; AltName: Full = Rheumatoid factor 10334859 creatine kinase mitochondrial 1B 265/3 ND precursor - The foregoing analyses demonstrate that a large number of target molecules can be detected in samples obtained using a polyethylene glycol based GLF.
- In this analysis, the ability of a magnesium citrate based GLF to support proteomic analysis was assessed. To identify target molecules in a GLF from a human subject administered a magnesium citrate-based lavage composition, a magnesium citrate-based lavage composition was administered to a subject; the GLF was collected from subject as part of a colonoscopy procedure. The proteome of the GLF was analyzed by mass spectroscopy as described in Example 1. Many different proteins were identified in the GLF. Examples of the most abundant identified proteins are listed in Table 6. Many of the identified proteins were detected with different colonoscopy preparations suggesting that the proteome is not dependent on the bowel preparation used.
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TABLE 6 Mascot score/ number of significant peptides NCBI gi # Protein Sample 27 4502085 pancreatic amylase alpha 2A precursor 2977/31 10280622 amylase pancreatic alpha-2B precursor 2891/30 40254482 salivary amylase alpha 1A precursor 2472/26 50363217 serine proteinase inhibitor clade A member 1 1316/12 236460050 elastase 3A pancreatic preproprotein 1299/13 15559207 elastase 2A preproprotein 1109/11 6679625 elastase 3B pancreatic preproprotein 987/10 29725633 regenerating islet-derived 1 alpha precursor 577/6 4507725 transthyretin precursor 570/7 4506147 protease serine 2 preproprotein 521/6 58331211 elastase 2B preproprotein 498/4 157266292 intestinal alkaline phosphatase precursor 491/7 113584 RecName: Full = Ig alpha-1 chain C region 338/3 125145 RecName: Full = Ig kappa chain C region 337/4 98986445 carcinoembryonic antigen-related cell adhesion 329/3 molecule 5 preproprotein 218512088 RecName: Full = Ig alpha-2 chain C region 293/3 41152086 serine (or cysteine) proteinase inhibitor clade B 293/4 (ovalbumin) member 6 4506145 protease serine 1 preproprotein 267/3 4502997 carboxypeptidase A1 precursor 146/3 - The foregoing analysis demonstrates that a large number of target molecules can be detected in samples obtained using a magnesium citrate based GLF.
- In this analysis, the ability of a samples obtained using a GLF in combination with SSL-7 enrichment to detect IgA-1 and IgA-2 antibodies was assessed. To identify target molecules in a GLF with affinity to Staphylococcus aureus superantigen-like protein 7 (SSL-7), a sulfate-based lavage composition (SUPREP) was administered to human subjects, and proteins were enriched in each GLF using SSL-7 affinity beads. The GLF was collected from subjects as part of a colonoscopy procedure.
- SSL-7 affinity beads were used to isolate IgA-1 and IgA-2 specifically. 20 μl of SSL-7 agarose (Invitrogen, San Diego, Calif.) was added to 1 ml of sample and incubated overnight on a roller at 4° C. Tubes were spun at 1,000×g for 2 minutes to pellet beads and supernatant discarded. Beads were washed 4× with 1× phosphate buffered saline and eluted with 20 μl of 100 mM glycine, pH 2.7 in a shaker for 1 hr at 600 rpm and 37° C. Eluted antibodies were diluted with 60 μl of 100 mM ammonium bicarbonate/10 mM tris (2-carboxyethyl) phosphine and digested with 2 μl of sequencing grade trypsin (Promega, Madison, Wis.). Mass spectrometry and database searches were performed as described above. The most abundant identified proteins present in GLF with affinity to SSL-7 and their corresponding Mascot scores are summarized in Table 7. As has been observed in prior examples, antibodies were present in the GLF and enrichment and analysis of these is possible using the affinity reagents, thus allowing the specific analysis of this subproteome in the GLF. The most abundant antibodies were IgAs. IgAs are consistently reported to be present in the intestinal tract.
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TABLE 7 Mascot score/ number of significant peptides NCBI gi # Protein Sample 12 Sample 13 113584 RecName: Full = Ig alpha-1 3084/29 2477/24 chain C region 31377806 polymeric immunoglobulin 3065/36 898/13 receptor precursor 218512088 RecName: Full = Ig alpha-2 chain 2736/24 2225/18 C region 125145 RecName: Full = Ig kappa chain 477/4 542/6 C region 21489959 immunoglobulin J chain 403/4 308/2 298351715 RecName: Full = Ig lambda-3 352/3 ND chain C regions 298351713 RecName: Full = Ig lambda-1 317/3 581/6 chain C regions 1684927 immunoglobulin light chain ND 636/6 - The foregoing analysis demonstrates that IgA antibodies can be detected in samples obtained using a GLF in combination with SSL-7 enrichment.
- In this analysis, the ability of samples obtained using a GLF in combination with Protein L enrichment to detect IgA and IgM antibodies was assessed. To identify target molecules in a GLF with affinity to Protein L, a sulfate-based lavage composition (SUPREP) was administered to human subjects, and proteins were enriched in each GLF using Protein L affinity beads. The GLF was collected from subjects as part of a colonoscopy procedure.
- Protein L affinity beads were used to isolate antibodies containing kappa light chains. 20 μl of Protein L agarose (Santa Cruz Biotechnology, Santa Cruz, Calif.) was added to 1 ml of sample and incubated overnight on a roller at 4 C. Tubes were spun at 1,000×g for 2 minutes to pellet beads and supernatant discarded. Beads were washed 4 times with 1× phosphate buffered saline and eluted with 20 μl of 100 mM glycine, pH 2.7 in a shaker for 1 hr at 600 rpm and 37° C. Eluted antibodies were diluted with 60 μl of 100 mM Ammonium bicarbonate/10 mM tris (2-carboxyethyl) phosphine and digested with 2 μl of sequencing grade trypsin (Promega, Madison, Wis.). The most abundant identified proteins present in GLF with affinity to Protein L and their corresponding Mascot scores and numbers of significant peptides are summarized in Table 8. As expected, IgA and associated chains from antibodies were again detected. As the Protein L is not totally specific for IgA antibodies, an IgM antibody (gi# 193806374) was also detected.
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TABLE 8 Mascot score/ number of significant peptides NCBI gi # Protein Sample 9 113584 RecName: Full = Ig alpha-1 chain C region 1183/14 31377806 polymeric immunoglobulin receptor 1149/14 precursor 218512088 RecName: Full = Ig alpha-2 chain C region 985/11 125145 RecName: Full = Ig kappa chain C region 654/8 193806374 RecName: Full = Ig mu chain C region 407/5 187950123 immunoglobulin heavy chain variable 289/3 region 21489959 immunoglobulin J chain 249/2 - The foregoing analysis demonstrates that IgA and IgM antibodies can be detected in samples obtained using a GLF in combination with ProteinL enrichment.
- In this analysis, the ability of a GLF to facilitate detection of proteins of bacterial origin was assessed. To identify target molecules associated with bacteria in a GLF, a sulfate-based lavage composition (SUPREP) was administered to two human subjects; the resultant GLF was collected from the subjects as part of a colonoscopy procedure. Super Optimal Broth (SOB) media was inoculated with 100 μl from each GLF and incubated overnight at 37° C. and 220 rpm shaking. Pellets were lysed in a bead-beater in 8M urea and lysates were diluted to 2 M urea in 50 mM ammonium bicarbonate/10 mM tris (2-carboxyethyl) phosphine and digested with sequencing grade trypsin (Promega, Madison, Wis.). Data were acquired on the Orbitrap MS system using 120 mins runs as described earlier. A MGF search file was created and searched with the Mascot search engine (Matrix Science, UK) against the RefSeq database (http://www.ncbi.nlm nih.gov/RefSeq/) with the taxonomy specified as Eubacteria with a mass accuracy of 10 ppm for the parent ion (MS) and 0.6 Da for the fragment ions (MS/MS). The most abundant identified proteins present in GLF associated with bacteria and their corresponding Mascot scores and numbers of significant peptides are summarized in Table 9. In the sample shown, the bacterium that was cultured was Escherichia coli. Other samples show different bacteria showing that the lavage fluid still retains some of the gut bacteria.
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TABLE 9 Mascot score/ number of significant peptides NCBI gi # Protein Bacterial isolate 15834378 chaperonin GroEL [Escherichia coli] 1294/3 15803852 elongation factor Tu [Escherichia coli] 1244/7 157159481 Molecular chaperone DnaK 683/3 [Escherichia coli] 123444102 elongation factor Tu [Yersinia 518/3 enterocolitica subsp. enterocolitica 8081] - The foregoing analysis demonstrates that proteins of bacterial origin can be detected in samples obtained using a GLF.
- In order to further facilitate the identification of a large number of target proteins detectable in samples obtained using a GLF, the search files generated from the data acquired individually from 12 subjects were concatenated into a single search file and searched using the previously specified parameters for the Orbitrap data. Many proteins were analyzed in various GLF samples and proteins selected that (predominantly) had at least 3 unique significant peptides detected with thresholds of p<0.05 (Mascot score approximately 41). Only 3 listed proteins (gi's 5031863, 6466801 and 115430223) had less than 3 significant peptides but these had Mascot scores of approximately 400, well above the 95% confidence level for protein identification. Proteins identified in this combined analysis are listed in Table 10 along with reported origins of particular proteins and reported associated cancers. Table 10 also lists SEQ ID NO.s. for identified peptides that had Mascot scores of 40 or greater for each unique identified protein. Many identified proteins have been reported to be present in pancreatic juice. References listed in Table 10 are provided in this application.
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TABLE 10 Mascot score/ SEQ ID NO.s number of of peptides with Origin of significant Mascot scores detected Presence in NCBI gi# Detected protein peptides >40 protein pancreatic juice Associated cancer References 10835000 pancreatic lipase 6919/75 1-77 pancreas Yes pancreatic Friess (2003) precursor 4502085 pancreatic amylase 5766/60 78-137 pancreas Yes gastric Kang (2010) alpha 2A precursor 10280622 amylase, 5332/55 78-83, 85-100, pancreas Yes liver Koyama (2001) pancreatic, alpha- 102, 103, 105-108, 2B precursor 110-130, 133, 136-141 40254482 salivary amylase 4712/50 78-83, 86-90, pancreas/ Yes lung Tomita (1989) alpha 1A precursor 93-99, 102, 105-107, salivary gland 110-116, 118-126, 128, 129, 131, 133-135, 139, 140, 142, 143 236460050 elastase 3A, 4267/49 144-193 pancreas Yes lung Shimada (2002) pancreatic preproprotein 6679625 elastase 3B, 4123/44 145-147, 151-153, pancreas Yes pancreatic Gao (2010) pancreatic 155, 157, preproprotein 161-163, 165, 166, 168, 170, 172-176, 178, 179, 181, 183, 185, 186, 188, 191, 194-211 4506147 protease, serine, 2 3427/33 212-247 pancreas Yes pancreatic Gao (2010) preproprotein 118498350 chymotrypsin B2 2621/27 248-274 pancreas Yes pancreatic Miao (2008) 118498341 chymotrypsin B1 2527/26 248-256, 258-274 pancreas Yes general Miao (2008) 50363217 serine proteinase 2443/27 275-304 pancreas/liver Yes general Normandin inhibitor, clade A, (2010), Sato member 1 (2004), Zhou (2000) 15559207 elastase 2A 2351/25 305-331 pancreas Yes pancreatic Akakura (2001) preproprotein Yamamura (1989) 54607080 pancreatic 2143/24 332-355 pancreas Yes liver Matsugi (2007) carboxypeptidase B1 preproprotein 4506145 protease, serine, 1 2135/21 215, 217, 219, pancreas Yes pancreatic preproprotein 221, 222, 225, 228, 229, 231, 232, 236, 238-240, 242, 245, 356-361 148539844 deleted in 2129/23 362-385 epithelial, Yes pancreatic, Sasaki (2002), malignant brain pancreas brain, Kuramitsu (2006) tumors 1 isoform c lung, precursor colon, gastric 113584 RecName: Full = Ig 2065/19 386-405 antibody- Yes alpha-1 chain C heavy chain region IgA 4502997 carboxypeptidase 2026/25 334, 406-429 pancreas Yes pancreatic Matsugi (2007) A1 precursor 29725633 regenerating islet- 1799/17 430-444 pancreas Yes liver Cavard (2006) derived 1 alpha precursor 31377806 polymeric 1783/19 445-465 epithelial Yes endometrial DeSouza (2005) immunoglobulin receptor precursor 218512088 RecName: Full = Ig 1779/16 386-388, 390-392, antibody- Yes alpha-2 chain C 397, 399-404, heavy chain region 466-469 157266300 membrane alanine 1581/16 470-486 small intestine Yes breast Liang (2006) aminopeptidase precursor 119395750 keratin 1 1491/15 487-501 epithelial 62526043 chymotrypsin C 1407/16 502-517 pancreas Yes liver Wang (2011) preproprotein 98986445 carcinoembryonic 1140/11 518-528 epithelial Yes pancreatic, Sato (2004), Van antigen-related cell colon Gisbergen (2005) adhesion molecule 5 preproprotein 217416390 carboxypeptidase 1129/11 529-539 pancreas Yes pancreatic Matsugi (2007) A2 (pancreatic) precursor 4505847 phospholipase A2 1124/13 540-552 pancreas Yes colon, Belinsky (2007), group IB prostate Sved (2004) 125145 RecName: Full = Ig 1121/14 553-566 antibody- Yes kappa chain C light chain region 7669492 glyceraldehyde-3- 1106/11 567-578 epithelial/bacterial colon Egea (2007), phosphate Shin (2009) dehydrogenase 58331211 elastase 2B 1083/11 305, 307-310, pancreas preproprotein 312-314, 318, 324, 328, 331 105990514 filamin B, beta 1041/7 579-586 multiple cell prostate Harding (2006) (actin binding types protein 278) 4507725 transthyretin 952/11 587-597 Liver/serum Yes colon Fentz (2007) precursor protein 55956899 keratin 9 945/9 598-607 epithelial 170296790 mesotrypsin 907/8 214, 215, 218, pancreas breast Hockla (2010) isoform 1 219, 221, 224, preproprotein 226, 233, 608 10835248 regenerating islet- 903/9 430, 431, 433-437, pancreas Yes pancreatic Cui (2010) derived 1 beta 441 precursor 41152086 serine (or cysteine) 902/9 609-617 keritinocytes, Yes colon Krasnov (2009) proteinase muscle, lung, inhibitor, clade B liver, (ovalbumin), pancreas member 6 1684927 immunoglobulin 889/8 618-625 light chain 4505605 pancreatitis- 848/9 626-634 pancreas Yes pancreatic Rosty (2002) associated protein precursor 13489087 serine (or cysteine) 842/8 635-642 keritinocytes, Yes pancreatic Sato (2004) proteinase muscle, lung, inhibitor, clade B liver, (ovalbumin), pancreas member 1 226529917 triosephosphate 760/7 643-649 multiple cell Yes breast Tamesa (2009) isomerase 1 types isoform 2 298351713 RecName: Full = Ig 760/8 618-623, 625, antibody- lambda-1 chain C 650 light chain regions 47132620 keratin 2 713/7 487, 495, 651-655 epithelial 5080756 Human Fc gamma 706/4 656-659 BP [AA 1-2843] 195972866 keratin 10 676/5 660-664 epithelial Liver, Yang (2008), pancreatic Xiao (2010) 4502027 albumin 642/4 665-668 preproprotein 40255013 carcinoembryonic 614/5 522, 524, 669-671 epithelial Yes colon Van Gisbergen antigen-related cell (2005) adhesion molecule 6 (non-specific cross reacting antigen) 125819 RecName: Full = Ig 611/6 672-677 antibody- leukemia Kipps (1988) kappa chain V-III light chain region HIC; Flags: Precursor 154146262 Fc fragment of IgG 607/4 656-659 prostate Gazi (2008) binding protein 157266292 intestinal alkaline 605/7 678-684 small intestine liver Yamamoto phosphatase (1984) precursor 50845388 annexin A2 isoform 1 604/5 685-689 multiple cell liver Mohammad types (2008) 50659080 serpin peptidase 599/4 690-693 liver melanoma Wang (2010) inhibitor, clade A, member 3 precursor 51593090 mucin 13, 575/5 694-698 colon GI cancer Maher (2011) epithelial transmembrane 119220569 zymogen granule 567/4 699-702 pancreas Yes membrane glycoprotein 2 isoform 1 125817 RecName: Full = Ig 554/6 672-677 antibody- leukemia Kipps (1988) kappa chain V-III light chain region HAH; Flags: Precursor 151301154 mucin 6, gastric 544/3 703-705 10334859 creatine kinase, 533/5 706-711 mitochondria tissue Bark (1980) mitochondrial 1B damage precursor 223099 Ig Aalpha1 Bur 520/4 388, 391, 467-469 4504919 keratin 8 506/6 712-717 epithelial skin Yamashiro (2010) 119703753 keratin 6B 504/4 488, 653, 654, epithelial breast Millar (2009) 718 121039 RecName: Full = Ig 501/4 719-722 antibody- gamma-1 chain C heavy chain region 4507149 superoxide 498/5 723-727 epithelial/ Yes multiple Pham (2009) dismutase 1, mitochondria soluble 223942069 enterokinase 497/3 728-731 small intestine multiple Vilen (2008) precursor 153070262 meprin A alpha 497/4 732-735 small intestine colon Lottaz (1999) 157364974 sucrase-isomaltase 480/3 736-738 small intestine colon Gu (2006) 125797 RecName: Full = Ig 478/5 672-676, 739 antibody- kappa chain V-III light chain region SIE 38455402 lipocalin 2 463/4 740-743 Epithelial, Yes pancreatic, Sato (2004), Lin many cell breast, (2011), types endometrial, Mahadevin prostate (2011) 167857790 orosomucoid 1 446/6 744-750 serum/acute precursor phase protein 5031839 keratin 6A 441/3 653, 654, 718 epithelial breast Millar (2009) 32313593 olfactomedin 4 437/4 751-754 small intestine, pancreatic, Kobayashi precursor colon, colon (2007), Koshida pancreas (2007) 125803 RecName: Full = Ig 432/5 672-676 antibody- kappa chain V-III light chain region WOL 10835063 nucleophosmin 1 414/5 755-759 multiple cell liver, Kuramitsu isoform 1 types others (2006), Grisendi (2006) 75707587 immunoglobulin 414/4 673, 674, 676, antibody- light chain variable 760 light chain region 5031863 galectin 3 binding 414/2 761, 762 multiple cell Yes colon Bresalier (2004), protein types Kim (2011) 187960098 medium-chain acyl- 407/3 763-765 mitochondrial/ CoA bacterial dehydrogenase isoform b precursor 4503143 cathepsin D 407/4 766-769 multiple cell breast Wolf (2003) preproprotein types 106507261 pancreatic lipase- 393/2 1, 5, 770 pancreas Yes related protein 2 223718246 plastin 1 393/3 771-774 small pancreatic Terris (2002) intestine, colon, kidney 4885165 cystatin A 384/4 775-778 macrophages colon Kupio (1998), Kos (2000) 6466801 intestinal mucin 3 379/1 779 epithelial pancreatic Park (2003) 115430223 galectin 3 377/2 780, 781 multiple cell pancreatic Jiang (2008) types 19923195 carcinoembryonic 375/4 518, 670, 782, epithelial Yes multiple Gerstel (2011) antigen-related cell 783 adhesion molecule 1 isoform 1 precursor 19923748 dihydrolipoamide 362/3 784-786 mitochondria S- succinyltransferase (E2 component of 2-oxo-glutarate complex) 193806374 RecName: Full = Ig 361/4 787-790 antibody- mu chain C region heavy chain 16306550 selenium binding 361/3 791-793 ovarian, Huang (2006), protein 1 uterine, Zhang (2010), gastric, Zhang (2011), esophageal Silvers (2010) 33456 immunoglobulin M 353/4 794-797 antibody- chain heavy chain 125811 RecName: Full = Ig 319/3 760, 798 799 antibody- kappa chain V-III light chain region VG; Flags: Precursor 123843 RecName: Full = Ig 219/3 796, 800, 801 antibody- heavy chain V-III heavy chain region VH26; Flags: Precursor 563454 Ig heavy chain 206/3 802-804 antibody- (VH4) V region heavy chain (VDJ) - The foregoing analysis demonstrates that a large number of proteins can be detected in samples obtained using a GLF.
- In this analysis, the ability of fecal samples to support proteomic analysis was assessed. To identify target molecules in a fecal sample, no lavage composition was administered to a human subject. A fecal sample was collected from the subject during normal defecation using a collection container placed in the toilet. A small amount of the fecal (stool) sample was homogenized in 0.1% TFA and then centrifuged at 13000×g. The protein was precipitated with 6 volumes of acetone, resuspended in 0.1% TFA, extracted with an equal volume of chloroform, and then processed in a SPE column as described in Example 1. The most abundant identified proteins and their corresponding Mascot scores and number of significant peptides are summarized in Table 11. Proteins, largely with likely pancreatic origin, were detected in the sample indicating that, after discovery in GLF, stool is a source for the detection of these biomarker proteins. However, the sample did also contain a number of other non-human materials that make the analysis much more limiting, especially for discovery of biomarkers.
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TABLE 11 Mascot score/ number of significant peptides NCBI gi # Protein Sample Fecal 1 119395750 keratin 1 549/7 15559207 elastase 2A preproprotein 546/5 236460050 elastase 3A pancreatic 532/4 preproprotein 55956899 keratin 9 444/5 125145 RecName: Full = Ig kappa chain C 396/5 region 4506147 protease serine 2 preproprotein 344/4 4506145 protease serine 1 preproprotein 320/5 118498350 chymotrypsin B2 298/3 6679625 elastase 3B pancreatic 286/3 preproprotein - The foregoing analysis demonstrates that a large number of target molecules can be detected in fecal samples.
- In this analysis, the ability of glycans to be detected in samples obtained in samples using a GLF was assessed. To identify and analyze target molecules, including glycans, in a GLF, GLF was collected from a human subject. 1.8 mL GLF was added to 12 mL ice cold acetone and incubated for one hour to pellet the protein. The sample was centrifuged at 12,000×g for 15 minutes and the acetone removed. After washing with ice cold acetone, the pellet was resuspended in 0.1% TFA and passed through a 5 mL, syringe style, SepPak C2 column Proteins were eluted with 60% acetonitrile/40% 0.1% TFA. After removal of the solvent under vacuum, the protein fraction was redissolved in 100
μL 50 mM ammonium bicarbonate and deglycosylated with 2 μL PNGaseF overnight at 37° C. in a shaker. After quenching with 1 mL 0.1% TFA, the glycans were collected as the flow through fraction from a 1 mL, syringe style, SepPak C18 column using a vacuum manifold. The dried glycans were labeled with 4-ABEE (ethyl 4-aminobenzoate) by reductive amination by adding 25 μL derivatizing solution (90:10 MeOH:HAc containing 35mM ABEE and 100 mM 2-PB) to the dried glycans, and incubating at 65° C. for 2 hours. Excess ABEE was removed by adding 1 mL ethyl ether, vortexing and discarding the ether. After a second ether extraction, the sample was briefly put in the SpeedVac to remove any residual ether. The labeled glycans were then run on the HPLC and eluted between 20-25% acetonitrile from an Agilent C8 reverse phase column. This fraction was vacuum-dried, redissolved in 50 μL of 0.1% TFA and run on the Waters Q-TOF LC-ESI-MS for glycan analysis. The derivatized glycans eluted from the C18 reverse phase column on the Q-TOF MS at about 20-25% acetonitrile in 0.2% formic acid. The mass spectrometer was scanned in MS-only mode from m/z 150-2000 every second to acquire the derivatized glycan profile data.FIG. 1 summarizes these results, and depicts a graph of the relative abundance of various glycoprotein derived glycan structures present in a fraction of a gastrointestinal lavage fluid. As shown inFIG. 1 , some glycoprotein derived glycan structures include particular modifications that are associated with truncation of the chains. These modifications may be due to bacterial activity present in the GLF sample as it is known that bacteria can digest and consume glycans from proteins. However, such modifications can also be associated with disease, especially cancer where aberrant glycosylation has been linked to the disease. - The foregoing analysis demonstrates that a large number of glycans can be detected in samples obtained using a GLF.
- In this analysis, the ability of metabolites to be detected in samples obtained using a GLF was assessed. To identify and analyze target molecules, including metabolites, in a GLF, a magnesium citrate-based lavage composition was administered to human subjects, and the resultant GLF was analyzed for metabolites such as cholic acid and other bile salts. The resultant GLF was collected from the subjects as part of a colonoscopy procedure.
- 3 ml GLF was centrifuged at max speed for 20 minutes and the supernatant acidified with 0.1% TFA. The supernatant was applied to a C18 SPE column (Waters Sep-Pak), washed with 3 volumes of 0.1% TFA, and eluted with 50% ACN in 0.1% TFA. The elutant was dried by centrifugal lyophilization and re-dissolved in 500 μl 0.1% TFA.
- Data were acquired on a Waters Q-TOF mass spectrometer using input from an LC system. The A solvent contained 3% of B and 0.2% formic acid in water. The B solvent contained 3% of A and 0.2% formic acid in acetonitrile. Solvents were HPLC grade from Fisher. The starting solvent was 5% B and remained for 5 min and then changed to 40% by 25 min, 90% by 30 min, and then reset to5% at 36. The MS scanned over the mass range from m/z 100 m/z to 2000 every second. Data were acquired using the standard MassLynx software. The eluting compounds with the cholic acid peak marked are summarized in
FIG. 2 . A similar profile of peaks was observed on the Orbitrap instrument where the cholic acid peak was identified using a standard and MS/MS data. Metabolites including cholic acid were identified. - The foregoing analysis demonstrates that metabolites can be detected in samples obtained using a GLF.
- The term “comprising” as used herein is synonymous with “including,” “containing,” or “characterized by,” and is inclusive or open-ended and does not exclude additional, unrecited elements or method steps.
- All numbers expressing quantities of ingredients, reaction conditions, and so forth used in the specification are to be understood as being modified in all instances by the term “about.” Accordingly, unless indicated to the contrary, the numerical parameters set forth herein are approximations that may vary depending upon the desired properties sought to be obtained. At the very least, and not as an attempt to limit the application of the doctrine of equivalents to the scope of any claims in any application claiming priority to the present application, each numerical parameter should be construed in light of the number of significant digits and ordinary rounding approaches.
- The above description discloses several methods and materials of the present invention. This invention is susceptible to modifications in the methods and materials, as well as alterations in the fabrication methods and equipment. Such modifications will become apparent to those skilled in the art from a consideration of this disclosure or practice of the invention disclosed herein. Consequently, it is not intended that this invention be limited to the specific embodiments disclosed herein, but that it cover all modifications and alternatives coming within the true scope and spirit of the invention.
- Each of the following references is incorporated by reference herein in its entirety.
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- All references cited herein, including but not limited to published and unpublished applications, patents, and literature references, are incorporated herein by reference in their entirety and are hereby made a part of this specification. To the extent publications and patents or patent applications incorporated by reference contradict the disclosure contained in the specification, the specification is intended to supersede and/or take precedence over any such contradictory material.
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