US20180273889A1 - Cell seeding device and method - Google Patents
Cell seeding device and method Download PDFInfo
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- US20180273889A1 US20180273889A1 US15/994,564 US201815994564A US2018273889A1 US 20180273889 A1 US20180273889 A1 US 20180273889A1 US 201815994564 A US201815994564 A US 201815994564A US 2018273889 A1 US2018273889 A1 US 2018273889A1
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- membrane
- well
- anchor
- base
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
- C12M25/04—Membranes; Filters in combination with well or multiwell plates, i.e. culture inserts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
Definitions
- cells can flow around edges of the membrane and become deposited on edges or an opposite surface of the membrane. Further, cells can settle on the surface in a non-uniform manner creating a cell layer without a uniform distribution. Still further, if the membrane is not fixed in place, the membrane can shift, twist, fold, etc. which can affect uniformity of the seeding.
- a device in an exemplary embodiment, comprises an anchor, a tray including a well adapted to receive the anchor, and a cover adapted to engage the tray and cover the well.
- the tray includes a top surface surrounding an opening of the well.
- the tray may include a flange disposed at least partially around a periphery of the top surface.
- the flange may include at least one first thread.
- the top surface may include a first rim formed at least partially around the periphery of the top surface.
- the well includes at least one first sidewall and a base.
- the at least one first sidewall may be disposed approximately perpendicular to the base.
- the base may include a second rim formed at least partially around a periphery of an external surface.
- the anchor may include at least one second sidewall enclosing a space.
- a first cross-sectional area of the base may be approximately equal to a second cross-sectional area of the space.
- the anchor may include at least one tab disposed on the at least one sidewall. The at least one tab may be disposed in a same plane as the at least one sidewall.
- a device comprises an anchor including at least one first sidewall enclosing a space having X, Y, and Z dimensions, a tray including a well adapted to receive the anchor, the well having at least one second sidewall and a base having X and Y dimensions, and a cover adapted to engage the tray, wherein the X and Y dimensions of the space are approximately equal to the X and Y dimensions of the base such that when the anchor is in the well, at least one of the at least one first sidewall and at least one of the at least one second sidewall are in direct contact.
- a method of generating a membrane-cell matrix having a substantially uniform distribution of the cells on the membrane in at least two dimensions comprises providing a device comprising an anchor and a tray including a well adapted to receive the anchor, placing a membrane in the well, placing the anchor in the well at least partially in contact with the membrane, and filling the well at least partially with a medium containing cells.
- the method may further comprise removing the anchor from the well, and removing the membrane-cell matrix.
- FIG. 1B shows an exemplary embodiment of a sectional view of a tray of a cell seeding device according to the present invention
- FIG. 3A shows an exemplary embodiment of a bottom view of a cover of a cell seeding device according to the present invention
- FIGS. 1A and 1B show an exemplary embodiment of a tray 100 of a cell seeding device according to the present invention.
- the tray 100 includes a flange 105 having at least one thread 110 formed at least partially on its periphery.
- the flange 105 has a diameter of approximately 3.38 inches.
- the flange 105 is disposed around a top surface 115 of the tray 100 .
- the top surface 115 includes a first rim 120 formed at least partially on its periphery.
- the first rim 120 projects axially from the top surface 115 .
- the first rim 120 extends approximately 0.052 inches above the top surface 115 .
- a second rim 140 may be formed on an external surface of the base 135 .
- the second rim 140 may provide for separation between the base 135 and a surface on which the tray 100 is placed and further provide support for the tray 100 to prevent tipping.
- a frictional surface e.g., rubber, bumps, etc. may be formed on the second rim 140 to further enhance stability of the tray 100 .
- the anchor 200 includes a plurality of sidewalls 205 and at least one tab 210 formed on a surface of at least one of the sidewalls 205 . While the exemplary embodiment of the anchor 200 is described as having a plurality of sidewalls 205 , those of skill in the art will understand that a circular or elliptical anchor may have a single sidewall. Further, those of skill in the art will understand that the anchor 200 may be any shape and size and include any number of sidewalls.
- the angle of the lower portion 230 leaves a space for a periphery of the membrane which extends beyond a periphery of the lower portion 230 .
- an angle of the lower portion 230 relative to the upper portion 225 is approximately 10.3°.
- the cavity 305 may further include a sidewall 310 having a thread 320 formed on an interior surface which is adapted to engage the thread 110 on the tray 100 .
- rotation of the cover 300 relative to the tray 100 causes engagement (or disengagement) via the threads 110 , 320 .
- any other engagement mechanism e.g., friction, snap-fit, clips, hooks, etc. may be utilized to ensure that the cover 300 properly engages and disengages the tray 100 .
- An outer surface of the cover 300 may include one or more ribs 325 .
- the ribs 325 provide an abutment surface for a user's fingers when handling the cover 300 .
- the ribs 325 provide a surface to which the user can apply varying degrees of force to effect the rotation and engagement or disengagement of the cover 300 from the tray 100 .
- the exemplary embodiment of the cover 300 is described as having ribs 325 , those of skill in the art will understand that the at least one rib 325 may be formed on the tray 100 (in addition to, or instead of the cover 300 ), and that any other element (e.g., recesses, projections, handles, frictional surfaces, etc.) or combination of elements may be utilized.
- any other element e.g., recesses, projections, handles, frictional surfaces, etc.
- the device may be utilized to seed cells on a membrane.
- the device may be used to seed cells for a matrix-induced autologous chondrocyte implantation (MACI®) implant.
- the device may be used to seed a sponge matrix.
- the cover 300 is coupled to the tray 100 , the cover 300 is removed from the tray 100 .
- a membrane is then placed in the well 125 on or adjacent to the base 135 .
- the membrane may have dimensions (in the X and Y planes) equal to or less than the dimensions of the base 135 , and thus may be pre-fabricated or pre-cut to fit within the well 125 on the base 135 .
- the membrane may be smoothed (e.g., using forceps) to remove any air bubbles between the base 135 and the membrane.
- the anchor 200 is then placed in the well 125 and on top of the membrane. In an exemplary embodiment, the anchor 200 may be held by the tab 210 with fingers or forceps.
- cells are then placed into the well 125 .
- the cells may be mixed into a medium, e.g., Dulbecco's modified Eagle's medium (DMEM), and poured into the well 125 .
- DMEM Dulbecco's modified Eagle's medium
- most, if not all, of the mixture is confined within the sidewalls 205 of the anchor 200 and the membrane.
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Abstract
Description
- This application is a divisional application of U.S. application Ser. No. 14/088,999, filed Nov. 25, 2013, which is incorporated by reference in its entirety.
- When seeding cells on a surface of a membrane, several problems can arise. For example, cells can flow around edges of the membrane and become deposited on edges or an opposite surface of the membrane. Further, cells can settle on the surface in a non-uniform manner creating a cell layer without a uniform distribution. Still further, if the membrane is not fixed in place, the membrane can shift, twist, fold, etc. which can affect uniformity of the seeding.
- Therefore, an improved device and method is needed to overcome the problems encountered when seeding cells on a membrane.
- In an exemplary embodiment, a device according to the present invention comprises an anchor, a tray including a well adapted to receive the anchor, and a cover adapted to engage the tray and cover the well.
- In an exemplary embodiment, the tray includes a top surface surrounding an opening of the well. The tray may include a flange disposed at least partially around a periphery of the top surface. The flange may include at least one first thread. The top surface may include a first rim formed at least partially around the periphery of the top surface.
- In an exemplary embodiment, the well includes at least one first sidewall and a base. The at least one first sidewall may be disposed approximately perpendicular to the base. The base may include a second rim formed at least partially around a periphery of an external surface. The anchor may include at least one second sidewall enclosing a space. A first cross-sectional area of the base may be approximately equal to a second cross-sectional area of the space. The anchor may include at least one tab disposed on the at least one sidewall. The at least one tab may be disposed in a same plane as the at least one sidewall.
- In an exemplary embodiment, the well is at least partially transparent and the anchor is at least partially colored.
- In an exemplary embodiment, the cover includes a cavity adapted to receive the flange. The cavity may include at least one second thread adapted to engage the at least one first thread. The cavity may include a slot adapted to receive the first rim. The cover may include at least one rib formed on an outer surface.
- In an exemplary embodiment, a device according to the present invention comprises an anchor including at least one first sidewall enclosing a space having X, Y, and Z dimensions, a tray including a well adapted to receive the anchor, the well having at least one second sidewall and a base having X and Y dimensions, and a cover adapted to engage the tray, wherein the X and Y dimensions of the space are approximately equal to the X and Y dimensions of the base such that when the anchor is in the well, at least one of the at least one first sidewall and at least one of the at least one second sidewall are in direct contact.
- In an exemplary embodiment, a method of generating a membrane-cell matrix having a substantially uniform distribution of the cells on the membrane in at least two dimensions comprises providing a device comprising an anchor and a tray including a well adapted to receive the anchor, placing a membrane in the well, placing the anchor in the well at least partially in contact with the membrane, and filling the well at least partially with a medium containing cells. The method may further comprise removing the anchor from the well, and removing the membrane-cell matrix.
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FIG. 1A shows an exemplary embodiment of a top view of a tray of a cell seeding device according to the present invention; -
FIG. 1B shows an exemplary embodiment of a sectional view of a tray of a cell seeding device according to the present invention; -
FIG. 2A shows an exemplary embodiment of a perspective view of an anchor for a cell seeding device according to the present invention; -
FIG. 2B shows an exemplary embodiment of a sectional view of an anchor for a cell seeding device according to the present invention; -
FIG. 3A shows an exemplary embodiment of a bottom view of a cover of a cell seeding device according to the present invention; -
FIG. 3B shows an exemplary embodiment of a sectional view (along section A-A inFIG. 3A ) of a cover of a cell seeding device according to the present invention; and -
FIG. 3C shows an exemplary embodiment of a side view of a cover of a cell seeding device according to the present invention. - Exemplary embodiments of a cell seeding device according to the present invention may be used to seed cells on a membrane. For example, the cell seeding device may be utilized to achieve a uniform distribution of cells on a porous membrane. In an exemplary embodiment, a first uniform distribution is achieved on a first axis, a second uniform distribution is achieved on a second axis, and/or a third uniform distribution is achieved on a third axis.
- In an exemplary embodiment, a cell seeding device according to the present invention comprises a tray having a well, an anchor adapted to fit within the well, and a cover adapted to engage the tray and cover the well.
-
FIGS. 1A and 1B show an exemplary embodiment of atray 100 of a cell seeding device according to the present invention. Thetray 100 includes aflange 105 having at least onethread 110 formed at least partially on its periphery. In an exemplary embodiment, theflange 105 has a diameter of approximately 3.38 inches. Theflange 105 is disposed around atop surface 115 of thetray 100. In an exemplary embodiment, thetop surface 115 includes afirst rim 120 formed at least partially on its periphery. Thefirst rim 120 projects axially from thetop surface 115. In an exemplary embodiment, thefirst rim 120 extends approximately 0.052 inches above thetop surface 115. - In an exemplary embodiment, a
well 125 is formed in thetray 100. In an exemplary embodiment, thewell 125 includes a plurality ofsidewalls 130 and abase 135. Each of thesidewalls 130 may include an upper portion coupled to thetop surface 115 and a bottom portion coupled to thebase 135. While the exemplary embodiment of thewell 125 is described as having a plurality ofsidewalls 130, those of skill in the art will understand that a circular or elliptical well may have a single sidewall. Further, those of skill in the art will understand that thewell 125 may be any shape and include any number of sidewalls. - In an exemplary embodiment, the
sidewalls 130 are approximately perpendicular to thetop surface 115 and thebase 135. In an exemplary embodiment, thebase 135 is approximately 2 inches by approximately 1.612 inches, and thesidewalls 130 are approximately 1.28 inches from thebase 135 to thetop surface 115. - In an exemplary embodiment, a
second rim 140 may be formed on an external surface of thebase 135. Thesecond rim 140 may provide for separation between the base 135 and a surface on which thetray 100 is placed and further provide support for thetray 100 to prevent tipping. In an exemplary embodiment, a frictional surface (e.g., rubber, bumps, etc.) may be formed on thesecond rim 140 to further enhance stability of thetray 100. -
FIGS. 2A and 2B show an exemplary embodiment of ananchor 200 for a cell seeding device according to the present invention. In an exemplary embodiment, theanchor 200 is adapted to be slidably disposed in thewell 125. Thus, theanchor 200 is sized and shaped to fit within thewell 125. - In an exemplary embodiment, the
anchor 200 includes a plurality ofsidewalls 205 and at least onetab 210 formed on a surface of at least one of thesidewalls 205. While the exemplary embodiment of theanchor 200 is described as having a plurality ofsidewalls 205, those of skill in the art will understand that a circular or elliptical anchor may have a single sidewall. Further, those of skill in the art will understand that theanchor 200 may be any shape and size and include any number of sidewalls. In an exemplary embodiment, thesidewalls 205 enclose a space having a cross-sectional area (in the X-Y plane) which is equal to or less than a cross-sectional area (in the X-Y plane) of thebase 135 of thewell 125. - In an exemplary embodiment, the at least one
tab 210 is disposed approximately in-plane with thesidewall 205 and extends above an upper surface of the sidewall 205 a predetermined distance. In an exemplary embodiment, the predetermined distance is 0.4 inches. In an exemplary embodiment, thetab 210 has a frusto-conical shape having a broad portion coupled to thesidewall 205 and a narrowed portion displaced from thesidewall 205. Those of skill in the art will understand that thetab 210 may be any shape or size and disposed at any angle relative to thesidewall 205. In an exemplary embodiment, the predetermined distance is determined such that a portion of thetab 210 extends above thetop surface 115 but does not extend above thefirst rim 120. - As shown in
FIG. 2B , in an exemplary embodiment, thesidewall 205 has aninner surface 215 which is approximately parallel to a longitudinal axis L of the device. Thesidewall 205 further includes anouter surface 220 having anupper portion 225 and alower portion 230 which, in use, is adapted to engage a membrane in thewell 125. Theupper portion 225 is approximately parallel to the longitudinal axis L of the device, and thelower portion 230 is disposed at a non-parallel angle to theupper portion 225. In an exemplary embodiment, thelower portion 230 is angled radially toward the longitudinal axis L of the device. For example, the angle of thelower portion 230 leaves a space for a periphery of the membrane which extends beyond a periphery of thelower portion 230. In an exemplary embodiment, an angle of thelower portion 230 relative to theupper portion 225 is approximately 10.3°. - In an exemplary embodiment, a first set of
sidewalls 205 is approximately 1.99 inches long and a second set ofsidewalls 205 is approximately 1.6 inches long. - In an exemplary embodiment, the well 125 may be formed from a first material which is substantially transparent, and the
anchor 200 may be formed from a second material which has a color visible through the first material of thewell 125. For example, it may be beneficial if theanchor 200 is red so that a user of the device can ensure that theanchor 200 is properly situated within the well 125 and/or makes theanchor 200 easier to remove from the well 125. -
FIGS. 3A-C show an exemplary embodiment of acover 300 of a cell seeding device according to the present invention. Thecover 300 includes acavity 305 adapted to receive theflange 105 and thetop surface 115 of thetray 100. Thecavity 305 includes an interior surface including aslot 315 adapted to at least partially engage thefirst rim 120. The engagement of theslot 315 and thefirst rim 120 may facilitate alignment of thecover 300 and thetray 100 when being coupled together. In an exemplary embodiment, theslot 315 may be annular or any other shape or size to correspond to the shape and size of thefirst rim 120. - The
cavity 305 may further include asidewall 310 having athread 320 formed on an interior surface which is adapted to engage thethread 110 on thetray 100. Thus, rotation of thecover 300 relative to thetray 100 causes engagement (or disengagement) via thethreads threads cover 300 and thetray 100, those of skill in the art will understand that any other engagement mechanism (e.g., friction, snap-fit, clips, hooks, etc.) may be utilized to ensure that thecover 300 properly engages and disengages thetray 100. - An outer surface of the cover 300 (shown in
FIG. 3C ) may include one ormore ribs 325. Theribs 325 provide an abutment surface for a user's fingers when handling thecover 300. For example, when rotating thecover 300 relative to thetray 100, theribs 325 provide a surface to which the user can apply varying degrees of force to effect the rotation and engagement or disengagement of thecover 300 from thetray 100. While the exemplary embodiment of thecover 300 is described as havingribs 325, those of skill in the art will understand that the at least onerib 325 may be formed on the tray 100 (in addition to, or instead of the cover 300), and that any other element (e.g., recesses, projections, handles, frictional surfaces, etc.) or combination of elements may be utilized. - In an exemplary embodiment, the
anchor 200 may be formed integrally with thecover 300. In this exemplary embodiment, one or more stem elements (not shown) may extend from the inner surface of thecavity 305 to thetab 210 and/or thesidewall 205. - In an exemplary embodiment, the device may be utilized to seed cells on a membrane. For example, the device may be used to seed cells for a matrix-induced autologous chondrocyte implantation (MACI®) implant. In another example, the device may be used to seed a sponge matrix.
- If the
cover 300 is coupled to thetray 100, thecover 300 is removed from thetray 100. A membrane is then placed in the well 125 on or adjacent to thebase 135. The membrane may have dimensions (in the X and Y planes) equal to or less than the dimensions of thebase 135, and thus may be pre-fabricated or pre-cut to fit within the well 125 on thebase 135. The membrane may be smoothed (e.g., using forceps) to remove any air bubbles between the base 135 and the membrane. Theanchor 200 is then placed in the well 125 and on top of the membrane. In an exemplary embodiment, theanchor 200 may be held by thetab 210 with fingers or forceps. - In an exemplary embodiment, cells are then placed into the
well 125. For example, the cells may be mixed into a medium, e.g., Dulbecco's modified Eagle's medium (DMEM), and poured into thewell 125. In an exemplary embodiment, there is limited, if any, space between thesidewalls 205 of theanchor 200 and thesidewalls 130 of thewell 125. Thus, most, if not all, of the mixture is confined within thesidewalls 205 of theanchor 200 and the membrane. For example, in an exemplary embodiment in which the well 125 includes foursidewalls 130 and theanchor 200 includes foursidewalls 205, at least two of thesidewalls 130, 205 (parallel or perpendicular) are in direct contact while theanchor 200 is in thewell 125. Confining the mixture in this manner promotes uniform cell distribution over the membrane in the X-Y plane and preferably the Z-plane. Thecover 300 may then be coupled to thetray 100, and the device may be left undisturbed for a predetermined period of time (e.g., 2-3 days). - After the predetermined period of time, the
cover 300 is removed from thetray 100, and theanchor 200 is removed from the well 125. For example, the user may engage theanchor 200, e.g., via thetab 210, using fingers or forceps. When theanchor 200 is removed, the membrane-cell matrix may be removed. - Those of skill in the art will understand that modifications (additions and/or removals) of various components of the apparatuses, methods and/or systems and embodiments described herein may be made without departing from the full scope and spirit of the present invention, which encompass such modifications and any and all equivalents thereof.
Claims (17)
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US15/994,564 US20180273889A1 (en) | 2013-11-25 | 2018-05-31 | Cell seeding device and method |
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US14/088,999 US10011811B2 (en) | 2013-11-25 | 2013-11-25 | Cell seeding device and method |
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US20070166819A1 (en) * | 2006-01-16 | 2007-07-19 | Reliance Life Sciences Pvt. Ltd. | Device for culturing and transporting cells |
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GB1360949A (en) | 1971-03-20 | 1974-07-24 | Elbatainer Kunststoff | Screw cap closure for canister or similar large containers |
US3846241A (en) * | 1972-10-10 | 1974-11-05 | Y Faur | Medium for neisseria and method of cultivating neisseria |
US5635396A (en) | 1995-08-30 | 1997-06-03 | Becton, Dickinson And Company | Coverslip holder |
US6268147B1 (en) | 1998-11-02 | 2001-07-31 | Kenneth Loren Beattie | Nucleic acid analysis using sequence-targeted tandem hybridization |
US7473767B2 (en) | 2001-07-03 | 2009-01-06 | The Institute For Systems Biology | Methods for detection and quantification of analytes in complex mixtures |
WO2005071401A2 (en) | 2004-01-15 | 2005-08-04 | Chiron Corporation | Homogeneous multiplex assay for nucleic acid targets |
ES1072702Y (en) * | 2010-05-27 | 2010-12-17 | Betapack Sa | THREADED PLUG |
PT2625261T (en) | 2010-10-08 | 2018-05-18 | Naturin Viscofan Gmbh | Cell culture insert |
KR101164019B1 (en) | 2010-12-17 | 2012-07-18 | 한국생명공학연구원 | Container for mass culture of organism comprising virus-free seed potato |
KR101422345B1 (en) * | 2012-10-19 | 2014-07-22 | 한국생명공학연구원 | Dual structured cell culture dish |
US10011811B2 (en) | 2013-11-25 | 2018-07-03 | Vericel Corporation | Cell seeding device and method |
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- 2014-11-18 ES ES14810067T patent/ES2778104T3/en active Active
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2018
- 2018-05-31 US US15/994,564 patent/US20180273889A1/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060172412A1 (en) * | 2005-02-02 | 2006-08-03 | Coletica | Support device for cells culture |
US20070166819A1 (en) * | 2006-01-16 | 2007-07-19 | Reliance Life Sciences Pvt. Ltd. | Device for culturing and transporting cells |
Also Published As
Publication number | Publication date |
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WO2015077240A1 (en) | 2015-05-28 |
EP3074502A1 (en) | 2016-10-05 |
US10011811B2 (en) | 2018-07-03 |
EP3074502B1 (en) | 2020-01-08 |
ES2778104T3 (en) | 2020-08-07 |
US20150147809A1 (en) | 2015-05-28 |
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