US20180230098A1

US20180230098A1 - Compounds useful as ccr9 modulators

Info

Publication number: US20180230098A1
Application number: US15/953,052
Authority: US
Inventors: Rajagopal Bakthavatchalam; Manas Kumar Basu; Ajit Kumar Behera; Chandregowda Venkateshappa; Christopher Alexander Hewson; Sanjay Venkatachalapathi Kadnur; Sarkis Barret Kalindjian; Bheemashankar Kulkarni; Rohit Saxena; Juluri Suresh; Vellarkad Viswanathan; Mohd Zainuddin; Akila Parvathy Dharshinis; Rajenda Kristam
Original assignee: Norgine BV
Current assignee: Norgine BV
Priority date: 2013-12-23
Filing date: 2018-04-13
Publication date: 2018-08-16
Also published as: AU2014372638A1; JP2017501145A; EP3087069B1; WO2015097121A9; US9969687B2; EP3087069A1; US20170001959A1; WO2015097121A1

Abstract

The present invention relates to compounds useful as CCR9 modulators, to compositions containing them, to methods of making them, and to methods of using them. In particular, the present invention relates to compounds capable of modulating the function of the CCR9 receptor by acting as partial agonists, antagonists or inverse agonists. Such compounds may be useful to treat, prevent or ameliorate a disease or condition associated with CCR9 activation, including inflammatory and immune disorder diseases or conditions such as inflammatory bowel diseases (IBD).

Description

INTRODUCTION

BACKGROUND OF THE INVENTION

Chemokines are a family of structurally related small proteins released from a variety of different cells within the body (reviewed in Vinader et al, 2012, Future Med Chem, 4(7): 845-52). The name derives from their primary ability to induce chemotaxis and thereby attract multiple cells of the immune system to sites of inflammation or as a part of normal immune function homeostasis. Examples of the types of cells attracted by chemokines include monocytes, T and B lymphocytes, dendritic cells, natural killer cells, eosinophils, basophils and neutrophils. Chemokines, in addition to their primary role in inducing chemotaxis, are also able to cause activation of leukocytes at the site of inflammation—for example, but not limited to, causing degranulation of granulocytes, generation of super-oxide anions (oxidative burst) and up-regulation of integrins to cause extravasation. Chemokines initiate their biological activity through binding to and activation of cell surface receptors—chemokine receptors. Chemokine receptors belong to the G-coupled protein receptor (GPCR), 7-trans-membrane (7-TM) superfamily—comprising an extracellular N-terminus with 7 helical trans-membrane domains and an intracellular C-terminus. Traditionally, chemokines are considered to bind to their receptors in the 7-TM region—this binding leading to activation of the receptor and resulting in G-protein activation (and subsequent secondary messenger transmission) by the intracellular portion of the receptor.
CCR9 is a chemokine receptor shown to be expressed on circulating T lymphocytes (Zabel et al, 1999, J Exp Med, 190:1241-56) and, in contrast to the majority of human chemokine receptors, CCR9 currently has only a single ligand identified: CCL25, otherwise known as thymus-expressed chemokine (TECK) (Zabalos et al, 1999, J Immunol, 162: 5671-5). As CCL25 expression is limited to intestinal epithelium and the thymus (Kunkel et al, 2000, J Exp Med, 192(5): 761-8), this interaction has been demonstrated to be the key chemokine receptor involved in targeting of T lymphocytes to the intestine (Papadakis et al, 2000, J Immunol, 165(9): 5069-76). The infiltration of T lymphocytes into tissues has been implicated in a broad range of diseases, including, but not limited to, such diseases as asthma, rheumatoid arthritis and inflammatory bowel disease (IBD). Specific to IBD, it has been observed that CCR9+ CD4 and CD8 T lymphocytes are increased in disease alongside an increased expression of CCL25 that correlates with disease severity (Papadakis et al, 2001, Gastroenterology, 121(2): 246-54). Indeed, disruption of the CCR9/CCL25 interaction by antibody and small molecule antagonists of CCR9 has been demonstrated to be effective in preventing the inflammation observed in small animal models of IBD (Rivera-Nieves et al, 2006, Gastroenterology, 131(5): 1518-29 and Walters et al, 2010, J Pharmacol Exp Ther, 335(1):61-9). In addition to the IBD specific role for CCR9, recent data also implicates the CCR9/CCL25 axis in liver inflammation and fibrosis where increased expression of CCL25 has been observed in the inflamed liver of primary sclerosing cholangitis patients along with a concomitant increase in the numbers of CCR9+ T lymphocytes (Eksteen et al, 2004, J Exp Med, 200(11):1511-7). CCR9+ macrophages have also been observed in in vivo models of liver disease and their function proven with CCL25 neutralising antibodies and CCR9-knockout mice exhibiting a reduction in CCR9+ macrophage number, hepatitis and liver fibrosis (Nakamoto et al, 2012, Gastroenterol, 142:366-76 and Chu et al, 2012, 63^rdAnnual Meeting of the American Association for the Study of Liver Diseases, abstract 1209). Therefore, modulation of the function of CCR9 represents an attractive target for the treatment of inflammatory, immune disorder and other conditions and diseases associated with CCR9 activation, including IBD and liver disease.
In addition to inflammatory conditions, there is increasing evidence for the role of CCR9 in cancer. Certain types of cancer are caused by T lymphocytes expressing CCR9. For example, in thymoma and thymic carcinoma (where cancer cells are found in the thymus), the developing T lymphocytes (thymocytes) are known to express high levels of CCR9 and CCL25 is highly expressed in the thymus itself. In the thymus, there is evidence that the CCR9/CCL25 interaction is important for thymocyte maturation (Svensson et al, 2008, J Leukoc Biol, 83(1): 156-64). In another example, T lymphocytes from acute lymphocytic leukaemia (ALL) patients express high levels of CCR9 (Qiuping et al, 2003, Cancer Res, 63(19): 6469-77). While the role for chemokine receptors is not clear in the pathogenesis of cancer, recent work has indicated that chemokine receptors, including CCR9, are important in metastasis of tumours—with a potential therapeutic role suggested for chemokine receptor antagonists (Fusi et al, 2012, J Transl Med, 10:52). Therefore, blocking the CCR9/CCL25 interaction may help to prevent or treat cancer expansion and/or metastasis.
Inflammatory bowel diseases (IBD) are chronic inflammatory disorders of the gastrointestinal tract in which tissue damage and inflammation lead to long-term, often irreversible impairment of the structure and function of the gastrointestinal tract (Bouma and Strober, 2003, Nat Rev Immunol, 3(7):521-533) Inflammatory bowel diseases may include collagenous colitis, lymphocytic colitis, ischaemic colitis, diversion colitis, Behçet's disease (also known as Behçet's syndrome), indeterminate colitis, ileitis and enteritis but Crohn's disease and ulcerative colitis are the most common forms of IBD. Crohn's disease and ulcerative colitis both involve chronic inflammation and ulceration in the intestines, the result of an abnormal immune response. Chronic and abnormal activation of the immune system leads to tissue destruction in both diseases, although ulcerative colitis is generally limited to the rectum and colon, whereas Crohn's disease (also known as regional ileitis) extends deeper in the intestinal wall and can involve the entire digestive tract, from the mouth to the anus.
Up to one million Americans have inflammatory bowel disease, according to an estimate by the Crohn's and Colitis Foundation of America. The incidence of IBD is highest in Western countries. In North America and Europe, both ulcerative colitis and Crohn's disease have an estimated prevalence of 10-20 cases per 100,000 populations (Bouma and Strober, 2003).
The primary goal when treating a patient with IBD is to control active disease until a state of remission is obtained; the secondary goal is to maintain this state of remission (Kamm, 2004, Aliment Pharmacol Ther, 20(4):102). Most treatments for IBD are either medical or surgical (typically only used after all medical options have failed). Some of the more common drugs used to treat IBD include 5-aminosalicylic acid (5-ASA) compounds (such as sulfasalazine, mesalamine, and olsazine), immunosuppressants (such as azathioprine, 6-mercaptopurine (6-MP), cyclosporine A and methotrexate), corticosteroids (such as prednisone, methylprednisolone and budesonide), infliximab (an anti-TNFα antibody) and other biologics (such as adilumumab, certolizumab and natalizumab). None of the currently available drugs provides a cure, although they can help to control disease by suppressing destructive immune processes, promoting healing of intestinal tissues and relieving symptoms (diarrhoea, abdominal pain and fever).
There is a need to develop alternative drugs for the treatment of IBD, with increased efficacy and/or improved safety profile (such as reduced side effects) and/or improved pharmacokinetic properties. Treatment of IBD includes control or amelioration of the active disease, maintenance of remission and prevention of recurrence.
Various new drugs have been in development, including the aryl sulfonamide compound N-{4-chloro-2-[(1-oxidopyridin-4-yl)carbonyl]phenyl}-4-(1,1-dimethylethyl)-benzenesulfonamide, also known as Vercirnon or GSK1605786 (CAS Registry number 698394-73-9), and Vercirnon sodium. Vercirnon was taken into Phase III clinical development for the treatment of patients with moderate-to-severe Crohn's disease. Vercirnon is the compound claimed in U.S. Pat. No. 6,939,885 (Chemocentryx) and is described as an antagonist of the CCR9 receptor. Various other aryl sulfonamide compounds have also been disclosed as CCR9 antagonists that may be useful for the treatment of CCR9-mediated diseases such as inflammatory and immune disorder conditions and diseases; for example, see the following Chemocentryx patent applications, WO2004/046092 which includes vercirnon, WO2004/085384, WO2005/112916, WO2005/112925, WO2005/113513, WO2008/008374, WO2008/008375, WO2008/008431, WO2008/010934, WO2009/038847; also WO2003/099773 (Millennium Pharmaceuticals), WO2007/071441 (Novartis) and US2010/0029753 (Pfizer).
Thus a number of CCR9-modulating compounds are known and some are being developed for medical uses (see, for example, the review of CCR9 and IBD by Koenecke and Förster, 2009, Expert Opin Ther Targets, 13 (3):297-306, or the review of CCR antagonists by Proudfoot, 2010, Expert Opin Investig Drugs, 19(3): 345-55). Different classes of compounds may have different degrees of potency and selectivity for modulating CCR9. There is a need to develop alternative CCR9 modulators with improved potency and/or beneficial activity profiles and/or beneficial selectivity profiles and/or increased efficacy and/or improved safety profiles (such as reduced side effects) and/or improved pharmacokinetic properties.
We now provide a new class of compounds that are useful as CCR9 modulators and in particular as partial agonists, antagonists or inverse agonists of CCR9. The compounds of the invention may have improved potency and/or beneficial activity profiles and/or beneficial selectivity profiles and/or increased efficacy and/or improved safety profiles (such as reduced side effects) and/or improved pharmacokinetic properties. Some of the preferred compounds may show selectivity for CCR9 over other receptors, such as other chemokine receptors.
Such compounds may be useful to treat, prevent or ameliorate a disease or condition associated with CCR9 activation, including inflammatory and immune disorder diseases or conditions such as inflammatory bowel diseases (IBD).

SUMMARY OF THE INVENTION

The present invention provides a compound of Formula (I) or a salt or solvate thereof, including a solvate of such a salt:
in which:
R₁is selected from hydrogen, methyl, and ethyl;
X is selected from a direct bond and (CR₅R₆)_p;
p is 1, 2, 3, 4, or 5;
each R₅is independently selected from hydrogen, methyl, and fluoro;
each R₆is independently selected from hydrogen, methyl, and fluoro;
R₂is selected from hydrogen, optionally substituted aryl, optionally substituted heteroaryl, C_3-7cycloalkyl, and optionally substituted C_3-7heterocycloalkyl;
each R₃is independently selected from halo, cyano (CN), C_1-6alkyl, methanesulfonyl (SO₂CH₃), C_1-6alkoxy, haloalkyl, haloalkoxy, and C_3-7cycloalkyl;
n is 0, 1 or 2;
each R₄is Z_q1B;
m is 0, 1, 2 or 3;
q₁is 0, 1, 2, 3, 4, 5 or 6;
each Z is independently selected from CR₇R₈, O, C═O, SO₂, and NR₉;
each R₇is independently selected from hydrogen, methyl, ethyl, and halo;
each R₈is independently selected from hydrogen, methyl, ethyl, and halo;
each R₉is independently selected from hydrogen, methyl, and ethyl;
each B is independently selected from hydrogen, halo, cyano (CN), optionally substituted aryl, optionally substituted heteroaryl, optionally substituted cycloalkyl, and A;

A is

Q is selected from CH₂, O, NH, and NCH₃;
x is 0, 1, 2, 3 or 4, and y is 1, 2, 3, 4 or 5, the total of x and y being greater or equal to 1 and less than or equal to 5 (1≤x+y≤5).
It will be appreciated that the compounds of the invention may contain one or more asymmetrically substituted carbon atoms. The presence of one or more of these asymmetric centres (chiral centres) in a compound of Formula (I) can give rise to stereoisomers, and in each case the invention is to be understood to extend to all such stereoisomers, including enantiomers and diastereomers, and mixtures thereof (including racemic mixtures thereof).
Where tautomers exist in the compounds of Formula (I), we disclose all individual tautomeric forms and combinations of these as individual specific embodiments of the invention.
In addition, the invention is to be understood to extend to all isomers which are compounds with one or more isotopic substitutions. For example, H may be in any isotopic form, including ¹H, ²H(D), and ³H(T); C may be in any isotopic form, including ¹²C, ¹³C, and ¹⁴C; O may be in any isotopic form, including ¹⁶O and ¹⁸O; and the like.
It will be appreciated that the particular groups or substituents, the number of groups or substituents, and the position of substitution in compounds of Formula (I) are selected so as to avoid sterically undesirable combinations.
When present, each of the R₃and R₄groups may be attached at any suitable position. An R₃group may be para, meta or ortho to the sulfonamide, especially para or meta, and most preferably para. For example, when n is 1 then R₃is most preferably para to the sulfonamide. An R₄group may be para, meta or ortho to the sulfonamide, especially para or meta, and most preferably para. For example, when m is 1, then R₄is preferably meta or para to the sulfonamide, and most preferably para to the sulfonamide; and when m is 2, then most preferably one R₄group is meta to the sulfonamide and the other R₄group is para to the sulfonamide.
Certain compounds of the invention may act as prodrugs, or may be converted into prodrugs by known methods, and in each case the invention is to be understood to extend to all such prodrugs.
Except where otherwise stated, throughout this specification and claims, any of the following groups present in a compound of the invention or in an intermediate used for the preparation of a compound of the invention, is as defined below:

- an alkyl group is any branched or unbranched (straight chain) hydrocarbon, and may for example contain from 1 to 7 carbon atoms, especially from 1 to 6 carbon atoms;
- a cycloalkyl group is any monocyclic saturated hydrocarbon ring structure, and may for example contain from 3 to 7 carbon atoms, especially 3, 4, 5 or 6 carbon atoms;
- a heteroalkyl group is any alkyl group wherein any one or more carbon atoms is replaced by a heteroatom independently selected from N, O, S;
- a heterocycloalkyl group is any cycloalkyl group wherein any one or more carbon atoms is replaced by a heteroatom independently selected from N, O, S;
- an aryl group is any polyunsaturated, aromatic hydrocarbon group having a single ring or multiple rings which are fused together or linked covalently; aryl groups with up to 10 carbon atoms are preferred, particularly a monocyclic aryl group having 6 carbon atoms; examples of aryl groups include phenyl, biphenyl and naphthalene;
- a heteroaryl group is any aryl group wherein any one or more ring carbon atoms is replaced by a heteroatom independently selected from N, O, S; heteroaryl groups with 5 to 10 ring atoms are preferred, particularly a monocyclic heteroaryl group having 5 or 6 ring atoms; examples of heteroaryl groups include pyridyl, pyrazolyl, pyridazinyl, pyrrolyl, oxazolyl, quinolinyl and isoquinolinyl;
- a halo group is any halogen atom, and may for example be fluorine (F), chlorine (Cl) or bromine (Br), and especially fluorine or chlorine;
- a haloalkyl group is any alkyl group substituted with one or more halogen atoms, particularly 1, 2 or 3 halogen atoms, especially fluorine or chlorine;
- an alkoxy group is any Oalkyl group, especially OC_1-6alkyl;
- a haloalkoxy group is any Ohaloalkyl group, especially OC_1-6haloalkyl.

Except where otherwise stated, throughout this specification and claims, the phrase “optionally substituted” means unsubstituted or substituted by up to three groups (“optional substituents”) independently selected from OH, ═O or O⁻, NO₂, CF₃, CN, halo (such as Cl or F), CHO, CO₂H, C_3-7cycloalkyl, C_1-4alkyl (such as methyl), C_1-4alkoxy (such as —O-methyl, —O-ethyl), COC_1-4alkyl (such as —(CO)-methyl), COC_1-4alkoxy (such as —(CO)—O-methyl), and C_1-4haloalkoxy.
Except where otherwise stated, throughout this specification and claims, the term “prodrug” means a compound which, upon administration to the recipient, has very low activity or is inactive in its administered state but is capable of providing (directly or indirectly) an active compound or an active metabolite thereof. A prodrug is converted within the body into its active form which has medical effects.

DETAILED DESCRIPTION OF THE INVENTION

The compounds as defined above are useful as CCR9 modulators and in particular as partial agonists, antagonists or inverse agonists of CCR9. Such compounds may be useful to treat, prevent or ameliorate a disease or condition associated with CCR9 activation, including inflammatory and immune disorder diseases or conditions. Such diseases or conditions include inflammatory bowel diseases (IBD). In particular, the compounds as defined above may be useful to treat, prevent or ameliorate Crohn's disease and/or ulcerative colitis, and most particularly Crohn's disease.
The compounds as defined above are novel. Accordingly, the present invention provides a compound of Formula (I) as defined above or a salt or solvate thereof, including a solvate of such a salt, per se. In particular, the present invention provides a compound of Formula (I) as defined above or a pharmaceutically acceptable salt or solvate thereof, including a solvate of such a salt, per se. Most particularly, the present invention provides a compound of Formula (I) or a pharmaceutically acceptable salt thereof, per se.
In order to use a compound of Formula (I) or a salt or solvate thereof for therapy, it is normally formulated in accordance with standard practice as a composition.
Thus the invention also provides a composition comprising a compound of Formula (I) or a salt or solvate thereof, including a solvate of such a salt, together with an acceptable carrier. In particular, the invention provides a pharmaceutical composition comprising a compound of Formula (I) or a salt or solvate thereof, including a solvate of such a salt, together with a pharmaceutically acceptable carrier.
The invention further provides a compound according to the invention for use in therapy, specifically, for use in the treatment, prevention or amelioration of a disease or condition associated with CCR9 activation, including inflammatory and immune disorder diseases or conditions. Such diseases or conditions include: (1) Inflammatory bowel diseases (IBD) such as Crohn's disease, ulcerative colitis, collagenous colitis, lymphocytic colitis, ischaemic colitis, diversion colitis, Behçet's disease, indeterminate colitis, ileitis and enteritis; (2) allergic diseases such as systemic anaphylaxis or hypersensitivity responses, drug allergies, insect sting allergies and food allergies; (3) immune-mediated food allergies such as Coeliac (Celiac) disease; (4) autoimmune diseases, such as rheumatoid arthritis, fibromyalagia, scleroderma, ankylosing spondylitis, juvenile RA, Still's disease, polyarticular juvenile RA, pauciarticular juvenile RA, polymyalgia rheumatica, psoriatic arthritis, osteoarthritis, polyarticular arthritis, multiple scerlosis, systemic lupus erythematosus, type I diabetes, type II diabetes, glomerulonephritis, and the like; (5) psoriasis and inflammatory dermatoses such as dermatitis, eczema, atopic dermatitis, allergic contact dermatitis, urticaria and pruritus; (6) asthma and respiratory allergic diseases such as allergic asthma, allergic rhinitis, hypersensitivity lung diseases and the like; (7) vaginitis; (8) vasculitis; (9) spondyloarthropathies; (10) scleroderma; (11) graft rejection (including allograft rejection); (12) graft-v-host disease (including both acute and chronic); (13) other diseases in which undesired inflammatory responses are to be inhibited, such as atherosclerosis, myositis, neurodegenerative diseases (such as Alzheimer's disease), encephalitis, meningitis, liver diseases (such as liver inflammation, liver fibrosis, hepatitis, NASH), nephritis, sepsis, sarcoidosis, allergic conjunctivitis, otitis, chronic obstructive pulmonary disease, sinusitis, Behçet's disease and gout; (14) cancers, such as thymoma and thymic carcinoma, and acute lymphocytic leukemia (ALL, also known as acute lymphoblastic leukemia).
In particular, the invention provides a compound according to the invention for use to treat, prevent or ameliorate Crohn's disease and/or ulcerative colitis, and most particularly Crohn's disease.
The invention further provides the use of a compound of the invention for the treatment, prevention or amelioration of diseases or conditions as mentioned above; the use of a compound of the invention for the manufacture of a medicament for the treatment, prevention or amelioration of diseases or conditions as mentioned above; and a method of treating, preventing or ameliorating a disease or condition as mentioned above in a subject, which comprises administering an effective amount of a compound or a composition according to the invention to said subject. The subject to be treated according to the present invention is typically a mammal. The mammal is generally a human but may for example be a commercially reared animal or a companion animal.
A compound of Formula (I) may also be used as an intermediate in a method to synthesise another chemical compound, including but not limited to another compound of Formula (I);
as a reagent in an analytical method; as a research tool—for example, as a comparator compound in an assay, or during compound screening to assist in identifying and/or profiling a compound with similar or differing activity in the test conditions applied, or as a control in cell based, in vitro and/or in vivo test assays.
Preferred compounds of Formula (I) include those wherein any one or more of the following apply:

- R₁is hydrogen; and/or
- X is selected from a direct bond, CH₂, and CH₂CH₂; especially X is selected from a direct bond and CH₂, and/or
- p is 1, 2, or 3; especially p is 1 or 2; more especially p is 1; and/or
- R₂is selected from optionally substituted aryl, optionally substituted heteroaryl (particularly C_5-6heteroaryl), and optionally substituted C_3-7heterocycloalkyl; especially R₂is selected from optionally substituted aryl and optionally substituted heteroaryl; more especially R₂is selected from optionally substituted phenyl, optionally substituted pyridyl, optionally substituted thiophenyl, optionally substituted pyrazolyl, optionally substituted pyridonyl, optionally substituted pyrimidinyl, optionally substituted pyrazinyl, optionally substituted pyridazinyl, optionally substituted imidazolyl, optionally substituted thiazolyl, optionally substituted tetrazolyl, and optionally substituted 1,2,4-triazolyl, including pyridyl, thiophenyl, pyrazolyl, pyrimidinyl, imidazolyl, thiazolyl, tetrazolyl, and 1,2,4-triazolyl; most especially R₂is selected from optionally substituted phenyl, optionally substituted pyridyl, optionally substituted thiophenyl, optionally substituted pyrazolyl, and optionally substituted imidazolyl; preferred optional substituents are selected from OH, ═O, O⁻, CF₃, CN, halo (such as Cl or F), CO₂H, C_1-4alkyl, C_1-4alkoxy, COC_1-4alkyl (such as acetyl, CH₃CO), and COC_1-4alkoxy, especially optional substituents are selected from OH, O⁻, cyano (CN), methyl, ethyl, —O-methyl, —O-ethyl, —(CO)—O-methyl, and —(CO)—O-ethyl; most preferably R₂is selected from cyanophenyl, acetylphenyl, methoxy-phenyl, pyridine N-oxide, methyl-pyridine N-oxide, methoxy-pyridine N-oxide, ethoxy pyridine N-oxide, pyridyl, methoxy-pyridyl, ethoxy-pyridyl, methyl-pyridyl, cyano-pyridyl, thiophenyl, carboxy-thiophenyl, carboxymethyl-thiophenyl, pyrazolyl, methyl-pyrazolyl, imidazolyl, and methyl-imidazolyl; particularly R₂is pyridyl, methyl-pyridyl, methoxy-pyridyl, ethoxy-pyridyl, pyridine N-oxide, methyl-pyridine N-oxide, methoxy-pyridine N-oxide or ethoxy-pyridine N-oxide; and/or
- each R₃is independently selected from halo, cyano, C_1-3alkyl, C_1-3alkoxy, C_1-3haloalkyl, and cyclopropyl; especially each R₃is independently selected from chloro, cyano, methyl, methoxy (CH₃O), propoxy particularly isopropoxy (Oisopropyl), trifluoromethyl, and cyclopropyl; especially R₃is chloro or cyano; most especially R₃is chloro; and/or
- n is 0 or 1; especially n is 1; when n is 1, then the R₃group is preferably para to the sulfonamide; and/or
- R₄is Z_q1B and q₁is 0, each B is independently selected from halo, CN, optionally substituted aryl, optionally substituted heteroaryl, and A; especially each B is independently selected from halo, optionally substituted C_5-6heteroaryl (particularly unsubstituted C_5-6heteroaryl), C_5-6heterocycloalkyl (where B is A, and the total of x and y is 3 or 4); more especially each B is independently selected from chloro, fluoro, pyridyl, pyrazolyl, oxazolyl, isoxazolyl, imidazolyl, pyrrolyl, C_5-6heterocycloalkyl (where B is A, the total of x and y is 3 or 4, and Q is CH₂or O); most especially each B is independently selected from chloro (particularly 4-chloro), fluoro, cyano, pyridyl, pyrazolyl, oxazolyl, isoxazolyl, imidazolyl, pyrrolidinyl, piperidinyl, morpholinyl; particularly each B is independently selected from chloro (particularly 4-chloro), fluoro, cyano, oxazolyl; and/or
- R₄is Z_q1B and q₁is 1, 2 or 3, each Z is independently selected from C_1-3alkyl, each B is independently selected from halo, CN, optionally substituted aryl, optionally substituted heteroaryl, and A; especially each B is independently selected from halo, optionally substituted C_5-6heteroaryl (particularly unsubstituted C_5-6heteroaryl), C_5-6heterocycloalkyl (where B is A, and the total of x and y is 3 or 4); more especially each B is independently selected from chloro, pyridyl, pyrazolyl, oxazolyl, isoxazolyl, imidazolyl, pyrrolyl, C_5-6heterocycloalkyl (where B is A, the total of x and y is 3 or 4, and Q is CH₂or O); most especially each B is independently selected from chloro, pyridyl, pyrazolyl, oxazolyl, isoxazolyl, imidazolyl, pyrrolidinyl, piperidinyl, morpholinyl; and/or
- R₄is Z_q1B and q₁is 1, 2, 3, 4, 5, or 6, particularly q₁is 1, 2, or 4 (most particularly 1 or 2), each Z is independently selected from CR₇R₈, O, C═O, and SO₂(particularly CR₇R₈and O), each R₇is independently selected from hydrogen, methyl, and halo, each R₈is independently selected from hydrogen, methyl, and halo, B is selected from hydrogen, halo, and cyano (particularly hydrogen and halo); especially each R₄is independently selected from butyl (particularly tert-butyl), propyl (particularly iso-propyl), methyl, COCH₃, C(CH₃)(CH₃)CN, trifluoromethyl, trifluoromethoxy, difluoromethoxy, methoxy; more especially each R₄is independently selected from butyl (particularly tert-butyl), propyl (particularly iso-propyl), trifluoromethyl, and trifluoromethoxy; most especially each R₄is independently selected from butyl (particularly tert-butyl); and/or
- m is 1 or 2; especially m is 1; when m is 1, then R₄is preferably meta or para to the sulfonamide, and most preferably para to the sulfonamide; and when m is 2, then most preferably one R₄group is meta to the sulfonamide and the other R₄group is para to the sulfonamide; for example when m is 1, R₄may be tert-butyl, iso-propyl, trifluoromethyl, trifluoromethoxy, difluoromethoxy, or methoxy (especially R₄may be tert-butyl or trifluoromethoxy); for example when m is 2, the two R₄groups may be trifluoromethyl and chloro or the two R₄groups may be trifluoromethyl and fluoro or the two R₄groups may be trifluoromethyl and difluoromethoxy.

Examples of particularly preferred compounds of Formula (I) include those wherein:

- R₁is hydrogen; and
- X is selected from a direct bond and CH₂, and
- R₂is selected from optionally substituted pyridyl, optionally substituted phenyl, optionally substituted pyrazolyl, optionally substituted imidazolyl, and optionally substituted thiophenyl (wherein optional substituents are selected from O⁻, OCH₃, OC₂H₅, CH₃, carboxy, carboxymethyl and CN); and
- n is 1 and R₃is chloro or cyano, particularly chloro; and
- m is 1 and R₄is tert-butyl.

In preferred compounds of the invention, optionally substituted groups are those that are unsubstituted or substituted by one or two groups independently selected from OH, ═O or O⁻, NO₂, CF₃, CN, halo (such as Cl or F), CHO, CO₂H, C_3-7cycloalkyl, C_1-4alkyl (such as methyl, ETHYL), C_1-4alkoxy (such as —O-methyl, —O-ethyl), COC_1-4alkyl (such as —(CO)-methyl), COC_1-4alkoxy (such as —(CO)—O-methyl, —(CO)—O-ethyl), and C_1-4haloalkoxy. For example, R₂may be optionally substituted pyridyl (including pyridyl; pyridyl substituted by methoxy, ethoxy, methyl or cyano; pyridine N-oxide; pyridine N-oxide substituted by methoxy, ethoxy, methyl or cyano). When R₂is an optionally substituted aryl, each substituent may be ortho, meta or para to the point of attachment to X. When R₂is an optionally substituted heteroaryl, each substituent may be ortho, meta or para to the point of attachment to X, or may be attached to a heteroatom.
Example compounds of Formula (I) include compounds wherein X is a direct bond. Further example compounds of Formula (I) include compounds wherein X is CH₂.
For compounds of Formula (I), examples of preferred XR₂include those shown below plus XR₂groups wherein the aryl or heteroaryl groups shown below are further optionally substituted:
For compounds of Formula (I), when R₄is A (ie q₁is 0 and B is A), R₄is a C_3-7heterocycloalkyl containing one heteroatom (N) or two heteroatoms (N plus O or N, where the second N may be substituted with methyl). For example, A may be pyrrolidinyl, piperidinyl, or morpholinyl. The group A is attached through any of its carbon or nitrogen atoms, for example as follows:
Preferred compounds of Formula (I) include those wherein:

- R₁is hydrogen; and
- X is CH₂; and
- R₂is an optionally substituted heteroaryl, particularly unsubstituted heteroaryl, most particularly pyridyl; and
- n is 0 (so there is no R₃group present); and
- m is 1; and
- R₄is trifluoromethoxy.

Examples of such compounds are shown below:
Other preferred compounds of Formula (I) include those wherein:

- R₁is hydrogen; and
- X is a direct bond; and
- R₂is selected from optionally substituted aryl (particularly optionally substituted phenyl) and optionally substituted heteroaryl; for example, R₂is selected from cyanophenyl, acetylphenyl, pyridyl, methyl-pyridyl, methoxy-pyridyl, ethoxy-pyridyl, pyridine N-oxide, methyl-pyridine N-oxide, methoxy-pyridine N-oxide and ethoxy-pyridine N-oxide, thiophenyl, pyrazolyl, pyrimidinyl, and imidazolyl; preferably R₂is selected from cyanophenyl, acetylphenyl, pyridyl, methyl-pyridyl, methoxy-pyridyl, ethoxy-pyridyl, pyridine N-oxide, methyl-pyridine N-oxide, methoxy-pyridine N-oxide and ethoxy-pyridine N-oxide; most preferably R₂is selected from pyridyl, methyl-pyridyl, methoxy-pyridyl, ethoxy-pyridyl, pyridine N-oxide, methyl-pyridine N-oxide, methoxy-pyridine N-oxide and ethoxy-pyridine N-oxide; and
- n is 0 (so there is no R₃group present); and
- m is 2; and
- one R₄group is halo (particularly chloro or fluoro), and the other R₄group is trifluoromethyl.

- R₁is hydrogen; and
- X is a direct bond; and
- R₂is selected from optionally substituted aryl (particularly optionally substituted phenyl) and optionally substituted heteroaryl; for example, R₂is selected from cyanophenyl, acetylphenyl, pyridyl, methyl-pyridyl, methoxy-pyridyl, ethoxy-pyridyl, pyridine N-oxide, methyl-pyridine N-oxide, methoxy-pyridine N-oxide and ethoxy-pyridine N-oxide, thiophenyl, pyrazolyl, pyrimidinyl, and imidazolyl; preferably R₂is selected from cyanophenyl, acetylphenyl, pyridyl, methyl-pyridyl, methoxy-pyridyl, ethoxy-pyridyl, pyridine N-oxide, methyl-pyridine N-oxide, methoxy-pyridine N-oxide and ethoxy-pyridine N-oxide; most preferably R₂is selected from pyridyl, methyl-pyridyl, methoxy-pyridyl, ethoxy-pyridyl, pyridine N-oxide, methyl-pyridine N-oxide, methoxy-pyridine N-oxide and ethoxy-pyridine N-oxide; and
- n is 0 (so there is no R₃group present); and
- m is 1; and
- R₄is butyl (particularly tert-butyl), trifluoromethyl, trifluoromethoxy, or difluoromethoxy; preferably R₄is butyl (particularly tert-butyl), or trifluoromethoxy.

Examples of such compounds are shown below:
Particularly preferred compounds of Formula (I) include those wherein:

- R₁is hydrogen; and
- X is a direct bond or CH₂; and
- R₂is selected from optionally substituted aryl (particularly optionally substituted phenyl) and optionally substituted heteroaryl (particularly optionally substituted pyridyl, optionally substituted thiophenyl, optionally substituted pyrazolyl, optionally substituted pyrimidinyl, and optionally substituted imidazolyl); for example, R₂is selected from cyanophenyl, acetylphenyl, pyridyl, methyl-pyridyl, methoxy-pyridyl, ethoxy-pyridyl, pyridine N-oxide, methyl-pyridine N-oxide, methoxy-pyridine N-oxide, ethoxy-pyridine N-oxide, thiophenyl, pyrazolyl, pyrimidinyl, and methyl-imidazolyl; preferably R₂is selected from cyanophenyl, pyridyl, methyl-pyridyl, methoxy-pyridyl, ethoxy-pyridyl, pyridine N-oxide, methyl-pyridine N-oxide, methoxy-pyridine N-oxide, ethoxy-pyridine N-oxide, pyrazolyl, and methyl-imidazolyl; most preferably R₂is pyridyl, methyl-pyridyl, methoxy-pyridyl, ethoxy-pyridyl, pyridine N-oxide, methyl-pyridine N-oxide, methoxy-pyridine N-oxide and ethoxy-pyridine N-oxide; and
- n is 1; and
- R₃is halo (particularly chloro), or cyano; and
- m is 1; and
- R₄is butyl, particularly tert-butyl.

Examples of such compounds are shown below:
For example, particularly preferred compounds of Formula (I) are those wherein R₁is hydrogen, X is a direct bond or CH₂, R₂is a substituted pyridyl (particularly pyridine N-oxide or methyl-pyridine N-oxide), n is 1, R₃is halo (particularly chloro), or cyano, m is 1, and R₄is butyl (particularly ten-butyl). In particular, compounds of Formula (I) are those wherein R₁is hydrogen, X is a direct bond or CH₂, R₂is a substituted pyridyl (particularly pyridine N-oxide or methyl-pyridine N-oxide), n is 1, R₃is halo (particularly chloro), m is 1, and R₄is butyl (particularly ten-butyl).
It will be appreciated that, in the compounds described above:

- R₄is trifluoromethoxy when R₄is Z_q1B, q₁is 2, the first Z group is O, the second Z group is CR₇R₈, and each of R₇, R₈and B is fluoro;
- R₄is trifluoromethyl when R₄is Z_q1B, q₁is 1, Z is CR₇R₈, and each of R₇, R₈and B is fluoro;
- R₄is tert-butyl when R₄is Z_q1B, q₁is 2, the first Z group is CR₇R₈where each of R₇and R₈is methyl, the second Z group is CR₇R₈where each of R₇and R₈is hydrogen, and B is hydrogen;

R₄is isopropyl when R₄is Z_q1B, q₁is 1, the Z group is CR₇R₈where each of R₇and R₈is methyl, and B is hydrogen; or R₄is isopropyl when R₄is Z_q1B, q₁is 2, the first Z group is CR₇R₈where one of R₇and R₈is methyl and the other is H, the second Z group is CR₇R₈where each of R₇and R₈is hydrogen, and B is hydrogen;
R₄is methyl when R₄is Z_q1B, q₁is 1, the Z group is CR₇R₈where each of R₇and R₈is hydrogen, and B is hydrogen;

- R₄is difluoromethoxy when R₄is Z_q1B, q₁is 2, the first Z group is 0, the second Z group is CR₇R₈, one of R₇, R₈and B is hydrogen, and two of R₇, R₈and B are fluoro;
- R₄is methoxy when R₄is Z_q1B, q₁is 2, the first Z group is O, the second Z group is CR₇R₈where each of R₇and R₈is hydrogen, and B is hydrogen;
- R₄is carboxy-methyl, (CO)CH₃when R₄is Z_q1B, q₁is 2, the first Z group is CO, the second Z group is CR₇R₈where each of R₇and R₈is hydrogen, and B is hydrogen,
- R₄is methyl sulfonyl, SO₂CH₃when R₄is Z_q1B, q₁is 2, the first Z group is SO₂, the second Z group is CR₇R₈where each of R₇and R₈is hydrogen, and B is hydrogen,
- R₄is (CH₂)₃OCH₃when R₄is Z_q1B, q₁is 5, each of the first three Z groups and the fifth Z group is CR₇R₈where each of R₇and R₈is hydrogen, the fourth Z group is O, and B is hydrogen,
- R₄is C(CH₃)(CH₃)CN when R₄is Z_q1B, q₁is 1, the Z group is CR₇R₈where each of R₇and R₈is methyl, and B is cyano.

Specific compounds of the invention include the compounds of Formula (I) listed in Table 1, and any salt or solvate thereof, including a solvate of such a salt:

TABLE 1

Compound number	Structure

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

32

33

34

35

36

37

38

39

40

41

42

43

44

45

46

47

48

49

50

51

52

53

54

55

56

57

58

59

60

61

62

63

64

65

66

67

68

69

70

71

72

73

74

75

76

77

78

79

80

81

82

83

84

85

86

87

88

89

90

91

92

93

94

95

96

97

98

99

100

101

102

103

104

105

106

107

108

109

110

111

112

113

114

115

116

117

118

119

120

121

122

123

124

125

126

127

128

129

130

131

132

133

134

135

136

137

138

139

140

141

142

143

144

145

146

147

148

149

150

151

152

153

154

155

156

157

158

159

160

161

162

163

164

165

166

167

168

169

170

171

172

173

174

175

176

177

178

179

180

181

182

183

184

185

186

187

188

189

190

191

192

193

194

195

196

197

198

199

200

201

202

203

204

205

206

207

208

209

210

211

212

213

214

215

216

217

218

219

220

221

222

223

224

225

226

227

228

229

230

231

232

233

234

235

236

237

238

239

240

241

242

243

244

245

246

247

248

249

250

251

252

253

254

255

256

257

258

259

260

261

262

263

264

265

266

267

268

269

270

271

272

273

274

275

276

277

278

279

280

281

282

283

284

285

286

287

288

289

290

291

292

The compound of Formula (I) may be used as such, or in the form of a salt or solvate thereof, including a solvate of such a salt. Preferably a salt or solvate is one which is pharmaceutically acceptable.
Suitable salts of the compound of Formula (I) include metal salts, for example alkali metal or alkaline earth metal salts, for example sodium, potassium, calcium and magnesium salts; or salts with ammonia, primary, secondary or tertiary amines, or amino acids, for example mono-, di- or tri-alkylamines, hydroxyalkylamines, and nitrogen-containing heterocyclic compounds, for example isopropylamine, trimethylamine, diethylamine, tri(i-propyl)amine, tri(n-propyl)amine, ethanolamine, 2-dimethylaminoethanol, lysine, histidine, arginine, choline, caffeine, glucamine, procaine, hydrabamine, betaine, ethylenediamine, N-alkylglucamines, theobromine, purines, piperazine, piperidine, morpholine, n-alkyl piperidines, etc; or salts such as trifluoroacetic acid (TFA) salt. For example, pharmaceutically acceptable salts of a compound of Formula (I) include acid addition salts such as hydrochloride, hydrobromide, citrate, tartrate and maleate salts and salts formed with phosphoric and sulfuric acid. In another aspect suitable pharmaceutically acceptable salts are base salts such as an alkali metal salt for example sodium or potassium, an alkaline earth metal salt for example calcium or magnesium, or organic amine salt for example triethylamine.
Many organic compounds can form complexes with solvents in which they are reacted or from which they are precipitated or crystallized. These complexes are known as solvates. For example, a complex with water is known as a hydrate. Such solvates form part of the invention.
The compound of Formula (I) or its salt or solvate (including a solvate of such a salt) may itself act as a prodrug, or may be converted into a prodrug by known methods. A further aspect of the invention provides a prodrug of the compound of Formula (I) or its salt or solvate (including a solvate of such a salt). Pharmaceutically acceptable prodrugs are described in T. Higuchi and V. Stella (Prodrugs as novel delivery systems, vol 14 of the ACS Symposium Series), and in Edward B. Roche, ed. (Bioreversible carriers in drug design, American Pharm Assoc and Pergamon Press, 1987), both of which are incorporated herein by reference.
In one embodiment, a prodrug is a compound having a group that is cleavable from the molecule to generate a biologically active form. Thus the prodrug may be converted within the body into an active form or an active metabolite or residue thereof, due to the presence of particular enzymes or conditions that cleave the prodrug molecule. The cleavable group within the prodrug may be linked by any suitable bond, such as an ester bond or an amide bond (derived from any suitable amine, for example a mono-, di- or tri-alkylamine, or any of the amines mentioned above). For example, the prodrug may be an in vivo hydrolysable ester, such as an ester of a CO₂H group present in the compound of Formula (I) with any suitable alcohol, for example a C_1-6alkanol. Alternatively, it may be an ester of any —OH group present in the compound of Formula (I) with any suitable acid, for example any carboxylic or sulfonic acid. Prodrugs that are in vivo hydrolysable esters of a compound of Formula (I) are pharmaceutically acceptable esters that hydrolyse in the human body to produce the parent compound. Such esters can be identified by administering, for example intravenously, to a test animal, the compound under test and subsequently examining the test animal's body fluids. Suitable in vivo hydrolysable esters for carboxy include methoxymethyl and for hydroxy include formyl and acetyl, especially acetyl.
The present invention also provides a process for the preparation of a compound of Formula (I), which comprises reacting an anhydride (A) with a primary amine (B) to produce a phthalimide (C), reducing the nitro group in the phthalimide (C) to form an aminophthalimide (D), then:

- (i) converting the aminophthalimide (D) to a secondary sulfonamide (F) using a sulfonyl chloride (E), and optionally derivatising the secondary sulfonamide (F) to a tertiary sulfonamide (H); or
- (ii) converting the aminophthalimide (D) to a secondary amine (G), and converting the secondary amine (G) to a tertiary sulfonamide (H) using a sulfonyl chloride (E); and
- (iii) optionally adding appropriate substituents to an R₂, R₃, or R₄group of the secondary sulfonamide (F) or of the tertiary sulfonamide (H);
  as shown in Scheme 1 below, wherein R₁, X, R₂, R₃, n, R₄, and m have the meanings given for the general Formula (I) and Z is a halogen, most likely a bromine atom:

The anhydride A may be reacted with a primary amine of formula B in a solvent such as acetic acid, at an elevated temperature, in order to produce phthalimide C. The nitro group in this molecule is reduced to an amino group using a variety of possible reducing agents including stannous chloride in ethanol, iron powder in acetic acid or by hydrogenation utilizing metal catalysts such as Raney nickel, platinum IV oxide or palladium on carbon.
The aminophthalimide of formula D may either be converted to the secondary sulfonamide F which may then, if desired, be derivatised to the tertiary sulfonamide H or it may first be converted to the secondary amine G, before conversion to the tertiary sulfonamide H. Conversion of the compounds of formula D or G to the compounds of formula F or H respectively may be achieved by the use of a sulfonyl chloride E. This reagent is either used with a base such as pyridine in the presence or absence of a catalytic quantity of an agent such as dimethylaminopyridine and using a solvent such as dichloromethane, or by the use of sodium hydride as base in a dipolar aprotic solvent such as DMF prior to addition of the sulfonyl chloride. Conversion of the compounds of formula D or F to the compounds of formula G or H respectively may be achieved by the use of a base such as sodium hydride followed by the appropriate alkyl halide. In the event that R₂is a pyridine N-oxide, this may be prepared most conveniently from the corresponding pyridine as a final step by treating the pyridine with an oxidizing agent such as meta-chloroperoxybenzoic acid in a solvent such as dichloromethane.
It will be appreciated that many of the relevant starting materials are commercially available or may be made by any convenient method as described in the literature or known to the skilled chemist or described in the Examples herein. For example, compounds of the Formula A
are known or can be prepared by methods analogous to known methods; specific methods are disclosed in the Examples herein.
In a further aspect of the invention, there is provided an intermediate compound for use in the synthesis of a compound of Formula (I). There is further provided the use of an intermediate compound to synthesise a compound of Formula (I). Such intermediate compounds include the intermediate compounds I-CVI disclosed in the Examples herein and listed in Table 2.

	TABLE 2

	Intermediate compound number	Disclosed in Example number

	I	1-6, 8
	II	1, 26, 27
	III	1
	IV	1
	V	1, 2, 4, 6-9, 11-30
	VI	2, 9
	VII	2
	VIII	2
	IX	3
	X	3
	XI	3
	XII	3, 5
	XIII	4
	XIV	4
	XV	4
	XVI	5
	XVII	5
	XVIII	5
	XIX	6, 18
	XX	6
	XXI	6
	XXII	7
	XXIII	7
	XXIV	7, 27
	XXV	7, 9, 11-25, 28-30
	XXVI	7
	XXVII	7, 19
	XXVIII	8
	XXIX	8
	XXX	8
	XXXI	9
	XXXII	9
	XXXIII	11
	XXXIV	11
	XXXV	11
	XXXVI	12
	XXXVII	12
	XXXVIII	12
	XXXIX	13
	XL	13
	XLI	13
	XLII	14
	XLIII	14
	XLIV	14
	XLV	15
	XLVI	15
	XLVII	15
	XLVIII	15
	XLIX	16
	L	16
	LI	16
	LII	17
	LIII	17
	LIV	17
	LV	18
	LVI	18
	LVII	19
	LVIII	19
	LIX	20
	LX	20
	LXI	20
	LXII	21
	LXIII	21
	LXIV	21
	LXV	22
	LXVI	22
	LXVII	22
	LXVIII	22
	LXIX	23
	LXX	23
	LXXI	23
	LXXII	23
	LXXIII	23
	LXXIV	24
	LXXV	24
	LXXVI	24
	LXXVII	25
	LXXVIII	25
	LXXIX	25
	LXXX	26
	LXXXI	26
	LXXXII	26
	LXXXIII	26
	LXXXV	27
	LXXXVI	27
	LXXXVII	27
	LXXXVIII	27
	LXXXIX	27
	XC	27
	XCI	27
	XCII	28
	XCIII	28
	XCIV	28
	XCV	28
	XCVI	28
	XCVII	29
	XCVIII	29
	XCIX	29
	CC	29
	CI	29
	CII	30
	CIII	30
	CIV	30
	CV	30
	CVI	30

A resulting compound of the invention may be converted into any other compound of the invention by methods analogous to known methods. For example: a resulting compound of Formula (I) may be converted into a salt or solvate thereof; the oxidation state of an atom in a heterocyclic ring may be increased or decreased by oxidation or reduction using known methods; an ester may be converted to the corresponding acid by hydrolysis (eg using an aqueous hydroxide such as NaOH) or an acid maybe converted to a corresponding metal salt (eg using an aqueous metal hydroxide, such as NaOH to produce the sodium salt). During synthesis of any compound of the invention, protecting groups may be used and removed as desired. Thus in the Scheme above, as well as corresponding to the definitions in Formula I, R₁, R₂, R₃and R₄can also represent appropriately protected forms of these groups.
The amount of the compound of the invention which is required to achieve a therapeutic effect will, of course, depend upon whether the effect is prophylactic or curative, and will vary with the route of administration, the subject under treatment, and the form of disease being treated. It is generally preferable to use the lowest dose that achieves the desired effect. The compound of the invention may generally be administered at a dose of from 0.1 to 1500 mg/kg per day, preferably 0.1 to 500 mg/kg per day, typically from 0.5 to 20 mg/kg/day, for example about 3 mg/kg/day. Unit dose forms may conveniently contain an amount of compound of the invention which is effective at such dosage or as a multiple of the same, for example units containing 5 mg to 500 mg, usually around 10 mg to 200 mg.
For example, a pharmaceutical composition of this invention may be administered to humans so that, for example, a daily dose of 0.5 to 20 mg/kg body weight (and preferably of 0.5 to 3 mg/kg body weight) is received. This daily dose may be given in divided doses as necessary, the precise amount of the compound received and the route of administration depending on the weight, age and sex of the patient being treated and on the particular disease or condition being treated according to principles known in the art. Typically unit dosage forms may contain about 1 mg to 500 mg of a compound of Formula (I). For example, a unit dosage form containing up to 10 mg/kg may be given twice per day, such as 1.5 mg/kg twice per day or 5 mg/kg twice per day or 10 mg/kg twice per day.
The compound of the present invention may be administered one or more times per day, for example, two or three times per day, or even more often, for example, four or five times per day.
The compounds of this invention may be administered in standard manner for the disease or condition that it is desired to treat. For these purposes the compounds of this invention may be formulated by means known in the art into the required form. While it is possible for the active ingredient to be administered alone, it is preferable for it to be present in a suitable composition formulated as required. Suitable formulations according to the invention include those suitable for oral (including sub-lingual), parenteral (including subcutaneous, intradermal, intramuscular, intravenous, and intraarticular), nasal, inhalation, topical (including dermal, buccal, and sublingual), vaginal and rectal administration. The most suitable route may depend upon, for example, the nature and stage of the condition and disorder of the recipient.
For oral administration, the compounds can be formulated as liquids or solids. Forms suitable for oral administration include for example tablets, capsules, pills, lozenges, granulates, dragees, wafers, aqueous or oily solutions, suspensions, syrups, or emulsions.
Forms suitable for parenteral use include for example sterile aqueous or oily solutions or suspensions or sterile emulsions or infusions.
Forms suitable for nasal administration include for example drops, sprays and aerosols.
Forms suitable for inhalation include for example finely divided powders, aerosols, fine particle dusts or mists which may be generated by means of various types of metered dose pressurized aerosols, nebulizers or insufflators.
Forms suitable for topical administration to the skin include, for example, gels, creams, ointments, emulsions, pastes, foams or adhesive patches. For female patients, the composition may be in a form suitable for intravaginal administration.
Forms suitable for rectal administration include suppositories, rectal capsules and enema solutions.
Forms suitable for transdermal administration generally comprise an adjuvant that enhances the transdermal delivery of the compound of the invention. Suitable adjuvants are known in the art.
A pharmaceutical composition of the present invention may be in unit dosage form. Suitable oral unit dosage forms include those mentioned above. For administration by injection or infusion unit dosage forms include, for example, vials and ampoules. Unit dosage forms for topical administration to the skin include blister packs or sachets, each blister or sachet containing a unit dose of, for example, a gel, cream or ointment, for example, as described above. A metered dosing device may be provided, for example, a pump device, for dosing a predetermined volume of a topical composition, for example, a cream, ointment or gel. A preparation may provide delayed or sustained release, for example a depot preparation or an adhesive patch.
Preferred formulations are those suitable for oral administration, for example in the form of tablets, capsules, pills or the like, or in the form of solutions suitable for injection such as in water for injections BP or aqueous sodium chloride.
To make a composition according to the invention, suitable carriers are well known in the art and include pharmaceutical grade starch, mannitol, lactose, magnesium stearate, sodium saccharin, talcum, cellulose, glucose, sucrose, (or other sugar), magnesium carbonate, gelatin, oil, alcohol, detergents, emulsifiers or water (preferably sterile).
A liquid formulation will generally consist of a suspension or solution of the compound or physiologically acceptable salt in a suitable aqueous or non-aqueous liquid carrier(s), for example water, ethanol, glycerine, polyethylene glycol or an oil. The formulation may also contain a suspending agent, preservative, flavouring or colouring agent.
A composition in the form of a tablet can be prepared using any suitable pharmaceutical carrier(s) routinely used for preparing solid formulations. Examples of such carriers include magnesium stearate, starch, lactose, sucrose and microcrystalline cellulose.
A composition in the form of a capsule can be prepared using routine encapsulation procedures. For example, powders, granules or pellets containing the active ingredient can be prepared using standard carriers and then filled into a hard gelatin capsule; alternatively, a dispersion or suspension can be prepared using any suitable pharmaceutical carrier(s), for example aqueous gums, celluloses, silicates or oils and the dispersion or suspension then filled into a soft gelatin capsule.
Compositions for oral administration may be designed to protect the active ingredient against degradation as it passes through the alimentary tract, for example by an outer coating of the formulation on a tablet or capsule.
Conveniently the composition is in unit dose form such as a tablet or capsule.
In addition to the compounds of the present invention, the pharmaceutical composition of this invention may also contain, or be co-administered (simultaneously or sequentially) with, one or more pharmacological agents of value in treating one or more diseases or conditions referred to hereinabove. For example, pharmaceutical compositions as described above may also comprise one or more further active ingredients in addition to a compound of the invention, for example, a further active ingredient with efficacy in the treatment or prevention of IBD or of conditions associated with IBD.
The compounds of the invention are compounds which modulate at least one function or characteristic of mammalian CCR9, for example, a human CCR9 protein. The ability of a compound to modulate the function of CCR9 can be demonstrated in a binding assay (such as a ligand binding or agonist binding assay), a migration assay, a signaling assay (such as activation of a mammalian G protein, induction of rapid and transient increase in the concentration of cytosolic free calcium) and/or cellular response assay (such as stimulation of chemotaxis, exocytosis or inflammatory mediator release by leukocytes). In particular, compounds of the invention may be evaluated in one or more of the following assays: (1) human CCR9 FLIPR assay using recombinant cell lines expressing human CCR9 or MOLT-4 cells (for example, identifying active compounds as those having K_i≤10 μM, preferred compounds as those having K_i≤1 μM, and most preferred compounds as those having a K_i≤500 nM); (2) chemotaxis assay using MOLT-4 cells (for example, identifying active compounds as those having K_i≤10 μM, preferred compounds as those having K_i≤1 μM, and most preferred compounds as those having a K_i≤500 nM); (3) chemotaxis assay using mouse and rat thymocytes (for example, identifying active compounds as those having K_i≤1 μM, preferred compounds as those having K_i≤500 nM, and most preferred compounds as those having a K_i≤500 nM).
As previously outlined the compounds of the invention are CCR9 modulators, in particular they are partial agonists, antagonists or inverse agonists of CCR9. Each of the above indications for the compounds of the Formula (I) represents an independent and particular embodiment of the invention. Whilst we do not wish to be bound by theoretical considerations, some of the preferred compounds of the invention may show selective CCR9 modulation for any one of the above indications relative to modulating activity against any other particular receptor, including any other particular chemokine receptor (for example, CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR10, CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6, CXCR7, CX3CR1, XCR1, ChemR23 or CMKLR1); by way of non-limiting example they may show 100-1000 fold selectivity for CCR9 over activity against any other particular chemokine receptor.
The invention will now be illustrated but not limited by the following Examples. Each exemplified compound represents a particular and independent aspect of the invention.
Where optically active centres exist in the compounds of Formula (I), we disclose all individual optically active forms and combinations of these as individual specific embodiments of the invention, as well as their corresponding racemates.
Analytical TLC was performed on Merck silica gel 60 F₂₅₄aluminium-backed plates. Compounds were visualised by UV light and/or stained either with iodine, potassium permanganate or ninhydrin solution. Flash column chromatography was performed on silica gel (100-200 M) or flash chromatography. ¹H-NMR spectra were recorded on a Bruker Avance-400 MHz spectrometer with a BBO (Broad Band Observe) and BBFO (Broad Band Fluorine Observe) probe. Chemical shifts (d) are expressed in parts per million (ppm) downfield by reference to tetramethylsilane as the internal standard. Splitting patterns are designated as s (singlet), d (doublet), t (triplet), q (quartet), m (multiplet) and bs (broad singlet). Coupling constants (J) are given in hertz (Hz). LC-MS analyses were performed on either an Acquity BEH C-18 column (2.10×100 mm, 1.70 μm) or on a Acquity HSS-T3 column (2.10×100 mm, 1.80 μm) or Zorbax HD C18 (2.1×50 mm, 1.8 μm) using the Electrospray Ionisation (ESI) technique. Purity assessment for final compounds was based on the following 3 LCMS methods. Method 1 consisted of the following: Acquity BEH C-18 column 2.10 mm×100 mm, 1.70 μm. Mobile phase; A, 5 mM ammonium acetate in water; B, acetonitrile; gradient, 90% A to 10% A in 8 min with 10 min run time and a flow rate of 0.3 mL/min. Method 2 consisted of the following: Acquity HSS-T3 column 2.10 mm×100 mm, 1.8 μm. Mobile phase; A, 0.1% TFA in water; B, acetonitrile; gradient, 90% A to 10% A in 8 min with 10 min run time and a flow rate of 0.3 mL/min. Method 3 consisted of the following: Zorbax HD C18 column 2.10 mm×50 mm, 1.8 μm. Mobile phase; A, 0.01% acetic acid in 95% water and methanol; B, 0.01% acetic acid in 5% water and methanol; gradient, 100% A to 100% B in 4 min with 5 min run time and a flow rate of 0.3 mL/min.

Example 1

Synthesis of Compound 1 [4-tert-Butylphenylsulfonic acid (1,3-dioxo-2-(pyridin-3-yl)-2,3-dihydro-1H-isoindol-4-yl)-amide] and Compounds 2-11

Synthesis of III:

To a stirred solution of I (2.0 g, 10.36 mmol) in acetic acid (28 mL) was added compound II (0.37 g, 3.88 mmol) and the reaction mixture heated to a reflux for 18 hours. The reaction was cooled to room temperature and the acetic acid was removed under reduced pressure to obtain a crude product. This material was suspended in ethanol (5 mL), cooled and filtered to leave crude 4-nitro-2-(pyridin-3-yl)isoindoline-1,3-dione as a white solid (III; 2.5 g). MS (M+1): 270.1. This was used without further purification in the next step.

Synthesis of IV:

To a solution of crude III (2.0 g) in methanol (100 mL) under nitrogen atmosphere was added 10% Pd/C (0.1 g). The reaction mixture was purged with hydrogen and stirred under hydrogen balloon pressure for 18 hours at room temperature, whereupon the reaction mixture was filtered through a celite bed under a nitrogen atmosphere and the solvent evaporated under reduced pressure to afford crude compound, 4-amino-2-(pyridin-3-yl) isoindoline-1, 3-dione, as a yellow solid (IV; 0.8 g,). MS (M+1): 240.1. The crude was carried forward to next step without purification.

Synthesis of 1; 4-tert-Butylphenylsulfonic acid (1,3-dioxo-2-(pyridin-3-yl)-2,3-dihydro-1H-isoindol-4-yl)-amide

A mixture of crude IV (100 mg) and pyridine (2 mL) was cooled to 0° C. tert-Butylbenzenesulfonyl chloride V (274 mg, 1.18 mmol) was added and the mixture stirred for 24 hours at room temperature. The reaction mixture was concentrated and to the residue obtained was added a saturated solution of ammonium chloride and extracted with dichloromethane. The organic layer was washed with brine solution & dried over anhydrous Na₂SO₄. This was filtered and the organic solvent was evaporated under reduced pressure to obtain crude compound. The crude material was purified using preparative TLC with 100% ethyl acetate as mobile phase to obtain 4-tert-butylphenylsulfonic acid (1,3-dioxo-2-(pyridin-3-yl)-2,3-dihydro-1H-isoindol-4-yl)-amide as a light yellow solid (1; 30 mg). ¹H NMR (400 MHz, DMSO-d6): δ 11.28 (bs, 1H), 8.59-8.56 (m, 2H), 7.83 (d, J=8.0 Hz, 2H), 7.76 (d, J=8.0 Hz, 2H), 7.54-7.58 (m, 4H), 7.43 (bs, 1H), 1.24 (s, 9H). MS (M−1): 434.2. (LCMS purity 98.14%, Rt=6.12 min (1)).
The following compounds were prepared in a similar manner using the appropriate sulfonyl chloride in the final step:


CPD		LCMS	Purity
number	Structure	(M + 1)	(LCMS)	¹H NMR

2	422.2	97.89%, Rt = 2.51 min (3)	¹H NMR (400 MHz, DMSO-d6): δ 11.28 (bs, 1H), 8.58 (t, J = 4.8 Hz, 2H), 7.84 (d, J = 8.0 Hz, 2H), 7.78 (d, J = 8.0 Hz, 2H), 7.58-7.44 (m, 5H), 2.94-2.91 (m, 1H), 1.16 (d, 6H).

3	445.2 (M − 1)	99.99%, Rt = 1.54 min (3)	¹H NMR (400 MHz, DMSO-d6): δ 8.56 (s, 1H), 8.52 (d, J = 3.6 Hz, 2H), 8.45 (s, 1H), 7.86-7.72 (m, 6H), 7.51-7.48 (m, 2H), 7.31 (s, 1H), 7.07 (d, J = 7.6 Hz, 1H).

4	448.1	99.99%, Rt = 2.32 min (3)	¹H NMR (400 MHz, CDCl3): δ 8.74 (s, 1H), 8.63 (d, J = 4.4 Hz, 1H), 8.15 (s, 1H), 8.07 (d, J = 8.0 Hz, 1H), 7.87 (t, J = 8.0 Hz, 2H), 7.80 (d, J = 8.4 Hz, 1H), 7.68 (t, J = 7.2 Hz, 2H), 7.53 (d, J = 8.0 Hz, 1H), 7.45 (q, J = 4.8 Hz, 1H).

5	464.1	99.99%, Rt = 2.37 min (3)	¹H NMR (400 MHz, DMSO-d6): δ 11.42 (s, 1H), 8.59 (m, 2H), 8.0 (d, J = 8.8 Hz, 2H), 7.88 (d, J = 8.4 Hz, 1H), 7.84 (d, J = 8.0 Hz, 1H), 7.60 (d, J = 7.2 Hz, 3H), 7.54 (m, 2H).

6	448.1	97.23%, Rt = 2.29 min (3)	¹H NMR (400 MHz, DMSO-d6): δ 11.51 (s, 1H), 8.60 (s, 1H), 8.58 (d, J = 4.4 Hz, 1H), 8.08 (d, J = 8.4 Hz, 2H), 8.00 (d, J = 8.4 Hz, 2H), 7.89 (d, J = 8.0 Hz, 1H), 7.84 (d, J = 8.4 Hz, 1H) 7.61 (1H, s), 7.56 (2H, m).

7	482.0	98.48%, Rt = 2.46 min (3)	¹H NMR (400 MHz, DMSO-d6): δ 11.47 (bs, 1H), 8.60 (s, 1H), 8.59 (d, J = 4.4 Hz, 1H) , 8.22 (s, 1H), 8.11 (d, J = 7.6 Hz, 1H), 7.97 (d, J = 8.4 Hz, 1H), 7.89 (d, J = 7.6 Hz, 1H), 7.85 (d, J = 8.0 Hz, 1H), 7.56 (m, 3H).

8	466.1	96.15%, Rt = 5.52 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.62 (bs, 1H), 8.64-8.60 (m, 2H), 8.42-8.42 (m, 1H), 8.37-8.34 (m, 1H), 7.86-7.78 (m, 3H), 7.76-7.73 (m, 1H), 7.65-7.63 (m, 1H), 7.61- 7.58 (m, 1H).

9	405.1	98.98%, Rt = 5.39 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.53 (bs, 1H), 8.63-8.60 (m, 2H), 8.14-8.08 (m, 4H), 7.86-7.83 (m, 1H), 7.81-7.79 (m, 1H), 7.72-7.70 (m, 1H), 7.61-7.57 (m, 2H).

10	398.05	96.25%, Rt = 5.04 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.15 (bs, 1H), 8.62 (s, 2H), 8.09- 8.07 (m, 2H), 7.87-7.79 (m, 2H), 7.68-7.66 ( m, 3H), 7.48-7.44 (m, 2 H)

11	414.11	97.56%, Rt = 5.19 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.24 (bs, 1H), 8.63-8.62 (m, 2H), 8.00-7.98 (d, J = 8.8 Hz, 2H), 7.87- 7.80 (m, 2H), 7.71-7.69 (m, 3H), 7.64-7.58 (m, 2 H).

Example 2

Synthesis of Compound 12 [4-(tert-butyl)-N-(1,3-dioxo-2-(pyridin-3-ylmethyl)isoindolin-4-yl)benzenesulfonamide] and Compounds 13-31

Synthesis of VII:

To a stirred solution of compound I (500 mg, 2.59 mmol) in acetic acid (7 mL) was added compound VI (420 mg, 3.88 mmol) and heated to a reflux for 18 hours. The reaction mixture was cooled to room temperature and the acetic acid was removed under reduced pressure to obtain a crude product. This material was suspended in ethanol (5 mL), cooled and filtered to obtain 4-nitro-2-(pyridin-3-ylmethyl)-isoindole-1,3-dione as a yellow solid (VII; 400 mg). ¹H NMR (400 MHz, CDCl₃): δ 8.57 (1H, s), 8.46 (d, J=4.0 Hz, 1H), 8.27 (d, J=8.0 Hz, 1H), 8.17 (d, J=7.2 Hz, 1H), 8.04 (t, J=7.8 Hz, 1H), 7.74 (d, J=8.0 Hz, 1H), 7.31-7.36 (m, 1H), 4.81 (s, 2H).

Synthesis of VIII:

To a solution of VII (400 mg) in methanol (80 mL) under nitrogen atmosphere was added 10% Pd/C (100 mg). The reaction mixture was purged with hydrogen and stirred under hydrogen balloon pressure for 18 hours at room temperature and then filtered through a celite bed under a nitrogen atmosphere and evaporated under reduced pressure to afford compound 4-amino-2-(pyridin-3-ylmethyl)-isoindole-1,3-dione as a yellow solid (VIII; 380 mg). MS (M+1): 254.5

Synthesis of 12; 4-(tert-butyl)-N-(1,3-dioxo-2-(pyridin-3-ylmethyl)isoindolin-4-yl)benzenesulfonamide

To a mixture of compound VIII (50 mg, 0.19 mmol) in dichloromethane (2 mL) was added pyridine (2 mL) and this solution was cooled to 0° C., compound V (96 mg, 0.39 mmol) was added and the solution was stirred for 24 hours at room temperature. The reaction mixture was concentrated under reduced pressure and diluted with saturated ammonium chloride solution and extracted with dichloromethane (2×10 mL). The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄and evaporated under reduced pressure to afford the crude compound which was purified by preparative HPLC to obtain 4-(tert-butyl)-N-(1,3-dioxo-2-(pyridin-3-ylmethyl)isoindolin-4-yl)benzenesulfonamide as a white solid (12; 50 mg, 56.8% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.82 (bs, 1H), 8.58 (s, 1H), 8.51 (d, J=4.0 Hz, 1H), 7.89 (d, J=8.8 Hz, 2H), 7.71-7.78 (m, 2H), 7.59 (d, J=8.4 Hz, 3H), 7.54 (d, J=7.2 Hz, 1H), 7.39-7.42 (m, 1H), 4.76 (s, 2H), 1.24 (s, 9H). MS (M+1): 450.2. (LCMS Purity 98.25%, Rt=2.56 min (3)).
The following compounds were prepared in a similar manner using the appropriate sulfonyl chloride in the final step:


Cmpd		LCMS	Purity
number	Structure	(M + 1)	(LCMS)	¹H NMR

13		461.7	99.18%, Rt = 1.99 min (3)	¹H NMR (400 MHz, DMSO-d6): δ 8.90 (bs, 1H), 8.67 (s, 1H), 8.56 (d, J = 4.8 Hz, 1H), 7.96 (d, J = 8.0 Hz, 3H), 7.89 (d, J = 8.0 Hz, 1H), 7.77 (d, J = 8.0 Hz, 1H), 7.74 (d, J = 8.8 Hz, 2H), 7.63 (t, J = 11.4 Hz, 1H), 7.50 (d, J = 8.8 Hz, 2H), 7.29-7.32 (m, 1H), 4.79 (s, 2H).

14		450.23	98.65%, Rt = 5.67 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 9.97 (s, 1H), 8.49 (s, 1H), 7.78-7.71 (m, 3H), 7.69-7.59 (m, 4H), 7.49- 7.45 (t, J = 7.8 Hz, 1H), 7.38-7.35 (m, 1H), 4.70 (s, 2H), 1.15 (s, 9H).

15		478.11	99.03%, Rt = 5.72 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.26 (bs, 1H), 8.54 (s, 1H), 8.49 (s, 1H), 8.07-8.05 (d, J = 8.4 Hz, 2H), 7.77-7.75 (m, 1H), 7.67-7.55 (m, 5H), 7.36-7.33 (m, 1H), 4.73 (s, 2H).

16		478.14	96.82%, Rt = 5.40 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.41 (bs, 1H), 8.51-8.49 (m, 2H), 7.92-7.90 (m, 1H), 7.81 (s, 1H), 7.78-7.74 (m, 1H), 7.72-7.68 (m, 1H), 7.66-7.62 (m, 3H), 7.58-7.56 (m, 2H), 7.38-7.34 (m, 1H), 4.71 (s, 2H).

17		478.11	98.56%, Rt = 5.74 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 9.98 (bs, 1H), 8.52-8.50 (m, 2H), 8.08-8.06 (d, J = 7.2 Hz, 1H), 7.78- 7.72 (m, 2H), 7.67-7.51 (m, 5H), 7.38-7.35 (m, 1H), 4.74 (s, 2H).

18		480.10	98.37%, Rt = 5.36 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.55 (bs, 1H), 8.53 (s, 1H), 8.49- 8.48 (d, J = 4.0 Hz, 1H), 8.31-8.30 (d, J = 6.4 Hz, 1H), 8.25-8.23 (m, 1H), 8.75-7.55 (m, 5H), 7.36-7.33 (m, 1H), 4.72 (s, 2H).

19		462.12	98.71%, Rt = 5.40 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.43 (bs, 1H), 8.54 (s, 1H), 8.49- 8.48 (d, J = 4.4 Hz, 1H), 8.14-8.12 (d, J = 8.4 Hz, 2H), 7.97-7.95 (d, J = 8.4 Hz, 2H), 7.77-7.73 (t, J = 7.8 Hz, 1H), 7.64 (m, 2H), 7.55-7.53 (d, J = 8.4 Hz, 1H), 7.36-7.33 (m, 1H), 4.73 (s, 2H).

20		493.92 (M − 1)	99.70%, Rt = 5.50 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.38 (bs, 1H), 8.54 (s, 1H), 8.49- 8.48 (d, J = 3.2 Hz, 1H), 8.38 (s, 1H), 8.17-8.15 (d, J = 8.8 Hz, 1H), 7.96-7.92 (m, 1H), 7.78-7.74 (m, 1H), 7.65-7.63 (m, 2H), 7.56-7.54 (m, 1H), 7.36-7.34 (m, 1H), 4.72 (s, 2H).

21		421.98 (M − 1)	99.44%, Rt = 5.50 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 9.74 (bs, 1H), 8.54 (s, 1H), 8.49- 8.48 (d, J = 3.2 Hz, 1H), 7.89-7.87 (d, J = 8.8 Hz, 2H), 7.74-7.70 (m, 1H), 7.68-7.66 (m, 1H), 7.60-7.53 (m, 2H), 7.36-7.33 (m, 1H), 7.08- 7.06 (d, J = 8.8 Hz, 2H), 4.74 (s, 2H), 3.80 (s, 3H).

22		462.09	98.44%, Rt = 5.50 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.48 (bs, 1H), 8.50 (s, 1H), 8.49- 8.48 (d, J = 1.2 Hz, 1H), 8.20 (s, 1H), 8.18-8.16 (d, J = 8.0 Hz, 1H), 8.02-8.0 (d, J = 8.0 Hz, 1H), 7.81- 7.74 (m, 2H), 7.64-7.61 (m, 2H), 7.58-7.56 (d, J = 8.0 Hz, 1H), 7.38- 7.34 (m, 1H), 4.71 (s, 2H).

23		408.11	98.78%, Rt = 5.82 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 9.78 (bs, 1H), 8.54 (s, 1H), 8.49- 8.48 (d, J = 4.0 Hz, 1H), 7.94-7.92 (d, J = 8.4 Hz, 1H), 7.73-7.67 (m, 2H), 7.61-7.53 (m, 3H), 7.45-7.37 (m, 3H), 4.74 (s, 2H), 2.62 (s, 3H).

24		436.09	98.38%, Rt = 4.93 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.29 (bs, 1H), 8.51-8.48 (m, 2H), 8.10-8.04 (m, 4H), 7.76-7.72 (m, 1H), 7.66-7.64 (m, 1H), 7.61-7.55 (m, 2H), 7.35-7.32 (m, 1H), 4.73 (s, 2H), 2.60 (s, 3H).

25		419.10	99.19%, Rt = 4.92 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.52 (bs, 1H), 8.53 (s, 1H), 8.50- 8.49 (m, 1H), 8.09-8.04 (m, 4H), 7.77-7.73 (m, 1H), 7.64-7.62 (m, 2H), 7.55-7.53 (d, J = 8.0 Hz, 1H), 7.38-7.35 (m, 1H), 4.73 (s, 2H).

26		462.13	99.39%, Rt = 5.66 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.04 (bs, 1H), 8.54-8.50 (m, 2H), 8.22-8.21 (d, J = 9.6 Hz, 1H), 8.03- 8.02 (d, J = 9.6 Hz, 1H), 7.88-7.84 (m, 2H), 7.77-7.73 (m, 1H), 7.68- 7.66 (m, 1H), 7.61-7.57 (m, 2H), 7.38-7.35 (m, 1H), 4.74 (s, 2H).

27		408.08	99.19%, Rt = 5.93 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 9.85 (bs, 1H), 8.53 (s, 1H), 8.49 (m, 1H), 7.83-7.81 (d, J = 8.4 Hz, 2H), 7.74-7.67 (m, 2H), 7.58-7.54 (m, 2H), 7.37-7.34 (m, 3H), 4.74 (s, 2H), 2.34 (s, 3H).

28		424.09	99.19%, Rt = 5.60 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.06 (bs, 1H), 8.54 (s, 1H), 8.50- 8.49 (m, 1H), 7.75-7.71 (m, 1H), 7.67-7.65 (m, 1H), 7.58-7.55 (m, 2H), 7.49-7.45 (m, 3H), 7.38-7.35 (m, 1H), 7.21-7.19 (m, 1H), 4.74 (s, 2H), 3.76 (s, 3H).

29		460.18	99.36%, Rt = 5.34 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.05 (bs, 1H), 8.64 (s, 1H), 8.58- 8.57 (d, J = 4.0 Hz, 1H), 8.03-8.01 (d, J = 8.8 Hz, 2H), 7.90-7.88 (m, 1H), 7.76-7.72 (t, J = 7.8 Hz, 1H), 7.60-7.09 (m, 6H), 4.80 (s, 2H).

30		412.20	98.72%, Rt = 5.19 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.08 (bs, 1H), 8.54 (s, 1H), 8.49 (m, 1H), 7.99 (m, 2H), 7.76-7.72 (t, J = 7.6 Hz 1H), 7.66-7.63 (d, J = 8.4 Hz, 1H), 7.60-7.56 (m, 2H), 7.42- 7.35 (m, 3 H), 4.73 (s, 2 H).

31		428.08	98.83%, Rt = 5.41 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.19 (bs, 1H), 8.55 (s, 1H), 8.49 (s, 1H), 7.94-7.90 (d, J = 8.8 Hz, 2H), 7.76-7.72 (m, 1H), 7.66-7.64 (m, 3H), 7.61-7.59 (m, 1H), 7.56-7.54 (m, 1H), 7.38-7.34 (m, 1H), 4.74 (s, 2H).

Example 3

Synthesis of Compound 32 [N-(1,3-dioxo-2-(pyridin-4-ylmethyl)isoindolin-4-yl)-4-(oxazol-5-yl)benzenesulfonamide]

Synthesis of X:

To a stirred solution of compound I (500 mg, 2.59 mmol) in acetic acid (7 mL) was added compound IX (419 mg, 3.88 mmol) and the reaction heated to a reflux for 18 hours. The reaction mixture was cooled to room temperature and the acetic acid removed under reduced pressure to obtain a crude product which was purified by suspension in ethanol (5 mL), cooling and filtration to obtain 4-nitro-2-pyridin-4-ylmethyl-isoindole-1,3-dione as a yellow solid (X; 400 mg). ¹H NMR (400 MHz, DMSO-d6): δ 8.49 (d, J=5.6 Hz, 2H), 8.29 (d, J=8.4 Hz, 1H), 8.25 (t, J=8.0 Hz, 1H), 8.19 (d, J=8.0 Hz, 1H), 7.34 (d, J=5.2 Hz, 2H), 4.80 (s, 2H).

Synthesis of XI:

To a solution of compound X (400 mg) in acetic acid (10 mL) was added iron powder (40 mg) and the reaction mixture was stirred for 1 hour at room temperature. The acetic acid was removed under reduced pressure to obtain a crude product. The reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄and evaporated under reduced pressure to afford 4-amino-2-pyridin-4-ylmethyl-isoindole-1,3-dione as a light brown solid (XI; 250 mg,). MS (M+1): 254.1

Synthesis of 32; N-(1,3-dioxo-2-(pyridin-4-ylmethyl)isoindolin-4-yl)-4-(oxazol-5-yl)benzenesulfonamide

To a mixture of sodium hydride (19 mg, 0.78 mmol) in DMF (2 mL) at 0° C. was added compound XI (50 mg, 0.19 mmol) and this was stirred for 15 minutes at 0° C. To the reaction mixture was further added compound XII (144 mg, 0.59 mmol) and this was stirred for 15 minutes at 0° C. and then allowed to warm to room temperature with constant stirring for 18 hours. The reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄and evaporated under reduced pressure to obtain the crude compound which was purified by preparative TLC using 100% ethyl acetate as mobile phase to afford N-(1,3-dioxo-2-(pyridin-4-ylmethyl)isoindolin-4-yl)-4-(oxazol-5-yl)benzenesulfonamide as a white solid (32; 20 mg, 22.2% yield). ¹H NMR (400 MHz, CDCl3): δ 8.89 (bs, 1H), 8.55 (d, J=5.6 Hz, 2H), 7.97 (d, J=6.8 Hz, 3H), 7.90 (d, J=8.4 Hz, 1H), 7.74 (d, J=8.4 Hz, 2H), 7.64 (t, J=7.8 Hz, 1H), 7.51-7.48 (m, 2H), 7.25-7.22 (m, 2H), 4.75 (s, 2H). MS (M+1) 461.1 (LCMS Purity 98.57%, Rt=2.39 min (3)).

Example 4

Synthesis of Compound 33 [N-(2-(4-acetylphenyl)-1,3-dioxoisoindolin-4-yl)-4-(tert-butyl)benzenesulfonamide] and Compound 34

Synthesis of XIV:

To a stirred solution of compound I (500 mg, 2.59 mmol) in acetic acid (7 mL) was added compound XIII (525 mg, 3.88 mmol) and the reaction mixture was heated to a reflux for 18 hours. The reaction mixture was cooled to room temperature and acetic acid was removed under reduced pressure to obtain crude product. This was suspended in ethanol (5 mL), cooled and filtered to get pure compound 2-(4-acetyl-phenyl)-4-nitro-isoindoline-1,3-dione as a yellow solid (XIV; 400 mg). ¹H NMR (400 MHz, DMSO-d6): δ 8.34 (d, J=8.0 Hz, 1H), 8.26 (d, J=7.6 Hz, 1H), 8.13-8.09 (m, 3H), 7.61 (d, J=8.4 Hz, 2H), 2.62 (s, 3H)

Synthesis of XV:

To a solution of compound XIV (400 mg) in acetic acid (30 mL) was added iron powder (50 mg) and the reaction mixture was stirred for 1 hour at room temperature. The acetic acid was removed under reduced pressure to obtain the crude product. The reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄and the organic solvent was evaporated under reduced pressure to afford 2-(4-acetyl-phenyl)-4-amino-isoindoline-1,3-dione as a yellow solid (XV; 250 mg). MS (M+1): 281.1

Synthesis of 33; N-(2-(4-acetylphenyl)-1,3-dioxoisoindolin-4-yl)-4-(tert-butyl)benzenesulfonamide

To a stirred mixture of compound XV (100 mg, 0.418 mmol) in dichloromethane (2 mL) was added pyridine (2 mL) at 0° C., followed by compound V (200 mg, 1.25 mmol). The reaction mixture was stirred for 24 hours at room temperature. The reaction mixture was concentrated under reduced pressure and diluted using saturated ammonium chloride solution and extracted with dichloromethane. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄and the organic solvent was evaporated under reduced pressure to obtain crude compound. Crude material was purified by preparative TLC using 100% ethyl acetate as mobile phase to afford N-(2-(4-acetylphenyl)-1,3-dioxoisoindolin-4-yl)-4-(tert-butyl)benzenesulfonamide as a yellow solid (33; 20 mg, 8.4% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.85 (bs, 1H), 8.07 (d, J=8.4 Hz, 2H), 7.90 (d, J=8.4 Hz, 2H), 7.76 (bs, 1H), 7.67 (d, J=8.4 Hz, 1H), 7.61-7.54 (m, 5H), 2.61 (s, 3H), 1.26 (s, 9H). MS (M+1) 477.2. (LCMS Purity 98.71%, Rt=2.80 min (3)).
The following compound was also prepared using a similar method and the appropriate sulfonyl chloride in the final step:


Cmpd		LCMS	Purity
number	Structure	(M + 1)	(LCMS)	¹H NMR

34		488.1	97.89%, Rt = 2.39 min (3)	¹H NMR (400 MHz, CDCl₃): δ 8.08 (d, J = 8.0 Hz, 2H), 8.01-7.99 (m, 4H), 7.79-7.79 (m, 3H), 7.61-7.54 (m, 3H), 7.49 (s, 1H), 2.64 (s, 3H).

Example 5

Synthesis of Compound 35 [N-(2-(4-cyanophenyl)-1,3-dioxoisoindolin-4-yl)-4-(oxazol-5-yl)benzenesulfonamide] and Compound 36

Synthesis of XVII:

To a stirred solution of compound I (500 mg, 2.59 mmol) in acetic acid (7 mL) was added compound XVI (458 mg, 3.88 mmol) and the reaction heated to reflux for 18 hours. The reaction mixture was cooled to room temperature and acetic acid was removed under reduced pressure to obtain the crude product. This material was suspended in ethanol (5 mL), cooled and filtered to leave 4-(4-nitro-1,3-dioxo-1,3-dihydro-isoindolin-2-yl)-benzonitrile as a yellow solid (XVII; 400 mg). ¹H NMR (400 MHz, DMSO-d6): δ 7.67 (d, J=8.4 Hz, 2H), 8.07 (d, J=8.4 Hz, 2H), 8.12 (t, J=8.0 Hz, 1H), 8.27 (d, J=6.8 Hz, 1H), 8.35 (d, J=8.0 Hz, 1H).

Synthesis of XVIII:

To a solution of compound XVII (400 mg) in acetic acid (10 mL) was added iron powder (100 mg) and the reaction mixture was stirred for 1 hour at room temperature. Acetic acid was removed under reduced pressure to obtain a crude product. The reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄and the organic solvent was evaporated under reduced pressure to afford 4-(4-amino-1,3-dioxo-1,3-dihydro-isoindolin-2-yl)-benzonitrile as a yellow solid (XVIII; 300 mg). ¹H NMR (400 MHz, DMSO-d6): δ 6.59 (s, 2H, s), 7.05 (t, J=8.0 Hz, 2H), 7.50 (d, J=7.6 Hz, 1H), 7.66 (d, J=8.4 Hz, 2H), 7.97 (d, J=8.4 Hz, 2H). MS (M+1) 264.07

Synthesis of 35; N-(2-(4-cyanophenyl)-1,3-dioxoisoindolin-4-yl)-4-(oxazol-5-yl)benzenesulfonamide

To a mixture of compound XVIII (100 mg, 0.418 mmol) in dichloromethane (2 mL) was added pyridine (2 mL) at 0° C. and then compound XII (200 mg, 1.25 mmol) was introduced. The reaction mixture was stirred for 24 hours at room temperature. This was concentrated under reduced pressure and diluted using saturated ammonium chloride solution and extracted with dichloromethane. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄and evaporated under reduced pressure to obtain the crude compound. This was purified by preparative TLC using 100% ethyl acetate as mobile phase to afford N-(2-(4-cyanophenyl)-1,3-dioxoisoindolin-4-yl)-4-(oxazol-5-yl)benzenesulfonamide as a white solid (35; 60 mg, 33% yield). ¹H NMR (400 MHz, DMSO-d6): δ 10.09 (s, 1H). 8.51 (s, 1H), 7.96-8.04 (m, 4H), 7.86-7.91 (m, 3H), 7.70-7.74 (m, 1H), 7.62 (t, J=7.6 Hz, 3H), 7.54-7.56 (m, 1H). MS (M+1) 471.1. (LCMS Purity 99.08%, Rt=2.45 min (3)).
The following compound was also prepared using a similar method and the appropriate sulfonyl chloride in the final step:


Cmpd		LCMS	Purity
number	Structure	(M + 1)	(LCMS)	¹H NMR

36		460.1	98.07%, Rt = 2.66 min (3)	¹H NMR (400 MHz, DMSO- d6): δ 9.0 (bs, 1H), 7.98 (d, J = 8.0 Hz, 1H), 7.86 (d, J = 8.0 Hz, 2H), 7.79 (d, J = 8.0 Hz, 2H), 7.69-7.73 (m, 1H), 7.62 (d, J = 12.0 Hz, 2H), 7.58 (d, J = 8.0 Hz, 1H), 7.51 (d, J = 8.0 Hz, 2H), 1.30 (s, 9H).

Example 6

Synthesis of Compound 37 [4-(tert-butyl)-N-(2-(3-cyanophenyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide] and Compounds 38-42

Synthesis of XX:

To a stirred solution of compound I (3 g, 15.54 mmol) in acetic acid (40 mL) was added compound XIX (2.7 g, 23.3 mmol) and the reaction heated to a reflux for 18 hours. The reaction mixture was cooled to room temperature and acetic acid was removed under reduced pressure to obtain a crude product. This was suspended in ethanol, cooled and filtered to afford 3-(4-nitro-1,3-dioxoisoindolin-2-yl)benzonitrile as an off white solid (XX; 3.3 g; 73% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.38-8.36 (d, J=8.0 Hz, 1H), 8.31-8.29 (d, J=7.6 Hz, 1H), 8.17-8.13 (t, J=8.0 Hz, 1H), 7.9 (m, 2H), 7.78 (m, 2H).

Synthesis of XXI:

To a stirred solution of compound XX (3.3 g, 11.26 mmol) in ethanol (70 mL) was added tin II chloride powder (27 g, 123 mmol) and the reaction mixture was heated to a reflux for 18 hours. The ethanol was concentrated under reduced pressure to obtain a crude product. The reaction mixture was diluted with water and a saturated solution with sodium bicarbonate and extracted with ethyl acetate. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄and evaporated under reduced pressure to afford 3-(4-amino-1,3-dioxoisoindolin-2-yl)benzonitrile as a yellow solid (XXI; 3 g). MS (M−1): 262.11

Synthesis of 37; 4-(tert-butyl)-N-(2-(3-cyanophenyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide

To a mixture of compound XXI (150 mg, 0.57 mmol) in pyridine (2 mL) was added 4-tert butylbenzenesulfonyl chloride (V; 264 mg, 1.14 mmol). The reaction mixture was stirred for 2 hours at 100° C. The reaction mixture was cooled and concentrated under reduced pressure and diluted using saturated ammonium chloride solution, which was extracted with dichloromethane. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄and evaporated under reduced pressure to obtain the crude compound, which was purified by column chromatography using 25% ethyl acetate in hexane to afford 4-(tert-butyl)-N-(2-(3-cyanophenyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide as an off white solid (37; 130 mg, 50% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.91 (bs, 1H), 7.94-7.90 (m, 4H), 7.80-7.62 (m, 7H), 1.27 (s, 9H). MS (M−1) 458.24 (LCMS Purity 96.81%, Rt=6.39 min (1)).
The following compounds were also prepared using a similar method and the appropriate sulfonyl chloride in the final step:


Cmpd		LCMS	Purity
number	Structure	(M − 1)	(LCMS)	¹H NMR

38		468.12	96.14%, Rt = 5.66 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.12 (bs, 1H), 8.08-8.05 (d, J = 8.4 Hz, 2H), 7.94-7.90 (m, 2H), 7.81-7.76 (m, 3H), 7.66- 7.64 (m, 2H), 7.39-7.22 (m, 3H).

39		470.14	99.73%, Rt = 5.55 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.50 (bs, 1H), 8.16-8.14 (d, J = 8.0 Hz, 2H), 8.0-7.98 (d, J = 8.0 Hz, 2H), 7.94-7.92 (m, 1H), 7.86 (s, 1H), 7.82-7.78 (m, 1H), 7.66-7.75 (m, 2H), 7.69-7.68 (m, 1H), 7.64-7.61 (m, 1H).

40		485.64	98.61%, Rt = 5.93 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.29 (bs, 1H), 8.12-8.09 (m, 2H), 7.93 (m, 1H), 7.87-7.83 (m, 2H), 7.76-7.72 (m, 3H), 7.66- 7.60 (m, 3H).

41		504.24	97.96%, Rt = 5.73 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.71 (bs, 1H), 8.42 (s, 1H), 8.21-8.19 (d, J = 6.8 Hz, 1H), 8.0-7.98 (d, J = 8.4 Hz, 1H), 7.93 (m, 1H), 7.87-7.83 (m, 2H), 7.77 (m, 3H), 7.64-7.62 (d, J = 8.4 Hz, 1H).

42		488.09	99.86%, Rt = 5.66 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.62 (bs, 1H), 8.43-8.42 (m, 1H), 8.35-8.33 (m, 1H), 7.95- 7.93 (m, 1H), 7.86-7.76 (m, 5H), 7.73-7.71 (m, 1H), 7.65-7.63 (m, 1H).

Example 7

Synthesis of Compound 43 [4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(pyridin-3-yl)isoindolin-4-yl)benzenesulfonamide]; Compound 44 [3-(4-(4-(tert-butyl)phenyl-sulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)pyridine 1-oxide] and Compounds 45-58:

Synthesis of XXIII:

To a stirred solution of compound XXII (10.0 g, 71.4 mmol) in water (125 mL) was added a catalytic amount of cetyl trimethylammonium bromide (0.01 g) and potassium permanganate (45.1 g, 280 mmol). The reaction mixture was heated to a reflux for 5 days. The reaction mass was filtered through a sintered funnel, whereupon the aqueous solution was acidified to pH 1. The aqueous solution was extracted with ethyl acetate. The organic layer was separated, dried using anhydrous Na₂SO₄and concentrated to afford 3-chlorophthalic acid as a yellow solid (XXIII; 5 g, 35% yield). ¹H NMR (400 MHz, DMSO-d6): δ 13.37 (bs, 2H), 7.90-7.88 (dd, J=7.6 Hz, 1H), 7.77-7.75 (d, J=7.6 Hz, 1H) 7.56-7.52 (t, J=7.8 Hz, 1H). MS (M−1): 198.96

Synthesis of XXIV:

Compound XXIII (5.0 g, 25.1 mmol) was added to a 1:2 solution of nitric acid and sulfuric acid (9 mL) at 0° C. The reaction mixture was stirred at room temperature for 3.5 hours. The reaction mixture was then cooled to 0° C. and crushed ice was added. The solid which precipitated out was filtered to afford 3-chloro-6-nitrophthalic acid as a yellow solid (XXIV; 5 g, 80% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.16-8.13 (d, J=8.8 Hz, 1H), 7.93-7.91 (d, J=8.8 Hz, 1H). MS (M−1): 244.06

Synthesis of XXV:

A stirred solution of compound XXIV (5.0 g; 20.3 mmol) in acetic anhydride (60 mL) was heated at 120° C. for 12 hours. The reaction mixture was cooled and diluted with water. The aqueous layer was extracted with ethyl acetate. The organic layer was washed with brine, dried over anhydrous Na₂SO₄and concentrated to afford 4-chloro-7-nitroisobenzofuran-1,3-dione as a yellow solid (XXV; 5 g).

Synthesis of XXVI:

To a stirred solution of compound XXV (5.0 g, 21.1 mmol) in acetic acid (62 mL) was added 2-aminopyridine (3.4 g, 34 mmol) and the reaction mixture heated at a reflux for 18 hours. The reaction mixture was cooled to room temperature and the acetic acid removed under reduced pressure to obtain crude product 4-chloro-7-nitro-2-(pyridin-3-yl)isoindoline-1,3-dione as a yellow solid (XXVI; 4.5 g,). MS (M+1): 304.02, which was used directly in the next step.

Synthesis of XXVII:

To a solution of compound XXVI (4.5 g) in ethanol (100 mL) under nitrogen atmosphere was added Raney nickel. The reaction mixture was purged with hydrogen and stirred under hydrogen balloon pressure for 6 hours at room temperature. The reaction mass was filtered through a celite bed under a nitrogen atmosphere and the solvent evaporated under reduced pressure to afford compound 4-amino-7-chloro-2-(pyridin-3-yl)isoindoline-1,3-dione as a yellow solid (XXVII; 1.3 g). ¹H NMR (400 MHz, DMSO-d6): δ 8.65 (s, 1H), 8.61-8.59 (d, J=4.8 Hz, 1H), 7.90-7.87 (d, J=4.8 Hz, 1H), 7.59-7.56 (m, 1H), 7.50-7.48 (m, 1H), 7.08-7.04 (m, 1H), 6.74-6.60 (m, 2H). MS (M+1): 274.04

Synthesis of 43; 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(pyridin-3-yl)isoindolin-4-yl)benzenesulfonamide

A mixture of compound XXVII (300 mg, 1.09 mmol) and pyridine (3 mL) was cooled to 0° C. and tert-butylbenzenesulfonyl chloride V (1.01 g, 4.1 mmol) was added together with a catalytic quantity of DMAP. The reaction mixture was stirred for 24 hours at 125° C. The reaction mixture was concentrated and to the resultant residue water was added and extracted with dichloromethane. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound. This was purified by preparative HPLC to afford 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(pyridin-3-yl)isoindolin-4-yl)benzenesulfonamide as a light yellow solid (43; 10 mg,). ¹H NMR (400 MHz, DMSO-d6): δ 10.0 (bs, 1H), 8.60 (m, 2H), 7.86-7.83 (m, 3H), 7.66-7.64 (m, 2H), 7.59-7.56 (m, 3H), 1.27 (s, 9H). MS (M+1): 470.2 (LCMS Purity 97.52%, Rt=6.17 min (1)).

Synthesis of 44; 3-(4-(4-(tert-butyl)phenylsulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)pyridine 1-oxide

To a stirred solution of 1-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(pyridin-3-yl)isoindolin-4-yl)benzenesulfonamide (43; 0.16 g, 0.34 mmol) in dichloromethane (5 mL), was added metachloroperoxybenzoic acid (0.059 g, 0.34 mmol). The reaction was stirred at room temperature for 8 hours whereupon the solvent was concentrated under reduced pressure and the residue diluted with water. The aqueous layer was extracted with dichloromethane. The combined organic layers were washed sequentially with sodium bicarbonate and brine, then dried over anhydrous Na₂SO₄, filtered and concentrated under vacuum to leave the crude compound which was triturated with hexane ether (1:1) mixture followed by prep TLC purification using 5% methanol in dichloromethane to afford 3-(4-(4-(tert-butyl)phenylsulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)pyridine 1-oxide (44; 0.060 g, 36% yield). ¹H NMR (400 MHz, DMSO-d6): δ 10.06 (bs, 1H), 8.33-8.31 (m, 2H), 7.94-7.92 (m, 2H), 7.83-7.81 (m, 1H), 7.69-7.57 (m, 4H), 7.45-7.43 (d, J=8.0 Hz, 1H), 1.27 (s, 9H). MS (M+1): 486.14. (LCMS purity 96.97%, Rt=5.34 min (1)).
The following compounds were prepared in a similar manner using the appropriate sulfonyl chloride in the penultimate step. This resulted either in the final pyridine compounds, which if required could also be converted into the corresponding pyridine N-oxides using mCPBA:


Cmpd		LCMS	Purity
number	Structure	(M + 1)	(LCMS)	¹H NMR

45		498.07	97.70% Rt = 5.54 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.37 (bs, 1H), 8.64-8.63 (d, J = 4 Hz, 1H), 8.59 (s, 1H), 8.11-8.09 (d, J = 8.8 Hz, 2H), 7.85-7.83 (d, J = 8.8 Hz, 2H), 7.65-7.58 (m, 4H).

46		514.11	98.81% Rt = 4.87 (1)	1H NMR (400MHz, DMSO-d6): δ 10.43 (bs, 1H), 8.32-8.31 (m, 2H), 8.11-8.09 (d, J = 8.8 Hz, 2H), 7.90- 7.80 (m, 1H), 7.65-7.57 (m, 4H), 7.43-7.41 (d, J = 8 Hz, 1H)

47		482.08	99.65% Rt = 5.25 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.52 (bs, 1H), 8.63-8.62 (d, J = 3.6 Hz, 1H), 8.58-8.57 (d, J = 2 Hz, 1H), 8.14-8.12 (d, J = 8 Hz, 2H), 7.98-7.96 (d, J = 8 Hz, 2H), 7.83- 7.81 (d, J = 8 Hz, 1H), 7.77-7.74 (m, 1H), 7.62-7.57 (m, 2H).

48		516.06	97.50% Rt = 5.48 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.79 (bs, 1H), 8.64-8.63 (d, J = 3.2 Hz, 1H), 8.58 (s, 1H), 8.42 (s, 1 H), 8.21-8.19 (d, J = 6.8 Hz, 1H), 7.99- 7.97 (d, J = 8.4 Hz, 1H), 7.83-7.81 (d, J = 8.4 Hz, 2H), 7.63-7.58 (m, 2H).

49		480.81	97.92% Rt = 4.76 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.22 (bs, 1H), 8.63-8.62 (d, J = 3.6 Hz, 1H), 8.60-8.59 (d, J = 2 Hz, 1H), 8.56 (s, 1 H), 8.07-8.05 (d, J = 8.4 Hz, 2H), 7.96-7.92 (m, 3H), 7.84-7.81 (m, 2H), 7.67-7.65 (d, J = 8.8 Hz, 1H), 7.59-7.56 (m, 1H).

50		444.09	98.76% Rt = 5.25 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.17 (bs, 1H), 8.65-8.62 (m, 2H), 7.88-7.80 (m, 2H), 7.64-7.51 (m, 5H), 7.27-7.25 (d, J = 8 Hz, 1H), 3.80 (s, 3H).

51		432.03	98.57% Rt = 5.05 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.20 (bs, 1H), 8.64-8.63 (d, J = 3.2 Hz, 1H), 8.61 (s, 1H), 8.05 (m, 2H), 7.86-7.80 (m, 2H), 7.65-7.60 (m, 2H), 7.49-7.44 (m, 2H).

52		480.04	99.23% Rt = 5.22 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.17 (bs, 1H), 8.64-8.61 (m, 2H), 8.06-8.04 (d, J = 9.2 Hz, 2H), 7.86- 7.80 (m, 2H), 7.66-7.64 (d, J = 8.8 Hz, 1H), 7.59 (m, 1H), 7.40-7.37 (m, 3H).

53		439.11	98.42% Rt = 4.66 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.63 (bs, 1H), 8.65-8.63 (d, J = 4.8 Hz, 1H), 8.60-8.59 (d, J = 2 Hz, 1H), 8.11 (m, 4H), 7.84-7.81 (m, 2H), 7.62-7.58 (m, 2H).

54		444.16	98.28% Rt = 5.39 min (1)	¹H NMR (400MHz, DMSO- d6-): δ 9.86 (bs, 1H), 8.64-8.62 (m, 2H), 7.94-7.92 (d, J = 8.8 Hz, 2H), 7.87- 7.85 (d, J = 7.6 Hz, 1H), 7.82-7.79 (d, J = 9.2 Hz, 1H), 7.68-7.66 (d, J = 8.8 Hz, 1H), 7.61-7.58 (m, 1H), 7.13-7.11 (d, J = 8.4 Hz, 2H), 3.82 (s, 3H),

55		500.09	99.79%, Rt = 5.28 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.72 (bs, 1H), 8.64-8.63 (d, J = 3.6 Hz, 1H), 8.59 (s, 1H), 8.43-8.42 (d, J = 5.3 Hz, 1H), 8.34-8.33 (m, 1H), 7.84-7.76 (m, 3H) 7.64-7.58 (m, 2H).

56		481.03	99.41%, Rt = 5.27 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.41 (bs, 1H), 8.64-8.60 (m, 2H), 8.06-8.04 (d, J = 8.4 Hz, 2H), 7.83- 7.82 (m, 2H), 7.78-7.76 (d, J = 8.4 Hz, 2H), 7.67-7.65 (m, 1H), 7.60- 7.58 (m, 1H), 1.68 (s, 6H).

57		456.35	99.36%, Rt = 5.94 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.0 (bs, 1H), 8.64-8.63 (d, J = 4.0 Hz, 1H), 8.61 (s, 1H), 7.93-7.91 (d, J = 8.4 Hz, 2H), 7.86-7.82 (m, 2H), 7.69-7.67 (d, J = 8.8 Hz, 1H), 7.60- 7.58 (m, 1H), 7.51-7.49 (d, J = 8.4 Hz, 2H), 3.01-2.94 (m, 1H), 1.19- 1.18 (d, J = 6.8 Hz, 6H).

58		497.36	95.01%, Rt = 4.52 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.30 (bs, 1H), 8.32-8.31 (m, 2H), 8.07-8.04 (d, J = 8.4 Hz, 2H), 7.84- 7.82 (d, J = 8.4 Hz, 1H), 7.78-7.76 (d, J = 8.4 Hz, 2H), 7.67-7.65 (d, J = 9.2 Hz, 1H), 7.61-7.57 (m, 1H), 7.44-7.42 (d, J = 8.0 Hz, 1H), 1.69 (s, 6H).

Example 8

Synthesis of 59 [4-(tert-butyl)-N-(2-(3-cyanobenzyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide] and Compounds 60-63:

Synthesis of XXIX:

To a stirred solution of compound I (0.5 g, 2.59 mmol) in acetic acid (10 mL) was added compound XXVIII (0.51 g, 3.88 mmol) and the reaction heated at 100° C. for 18 h. The reaction mixture was cooled to room temperature and acetic acid was removed under reduced pressure to obtain a crude product. This was suspended in ethanol, cooled and filtered to afford 3-((4-nitro-1,3-dioxoisoindolin-2-yl)methyl)benzonitrile as an off white solid (XXIX; 0.4 g; 55% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.31-8.29 (d, J=8 Hz, 1H), 8.20-8.18 (d, J=7.2 Hz, 1H), 8.08-8.05 (t, J=7.6 Hz, 1H), 7.85 (s, 1H), 7.76-7.70 (m, 2H), 7.57-7.53 (t, J=7.6 Hz, 1H), 4.84 (s, 2H). MS (M+1): 308.00.

Synthesis of XXX:

To a stirred solution of compound XXIX (0.4 g, 1.30 mmol) in ethanol (70 mL) was added stannous chloride powder (0.87 g, 3.9 mmol) and the reaction mixture was heated to a reflux for 18 h. The ethanol was concentrated under reduced pressure to obtain a crude product. The reaction mixture was diluted with water and a saturated solution of sodium bicarbonate and the aqueous layer extracted with ethyl acetate. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄and evaporated under reduced pressure to afford crude 3-((4-amino-1,3-dioxoisoindolin-2-yl)methyl)benzonitrile as a yellow solid (XXX; 0.25 g). MS (M−1): 276.09 which was used in the next step without further purification.

Synthesis of 59: 4-(tert-butyl)-N-(2-(3-cyanobenzyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide

To a mixture of compound XXX (0.25 g, 0.902 mmol) in pyridine (4 mL) was added 4-tert butylbenzenesulfonyl chloride (V; 0.73 g, 3.15 mmol). The reaction mixture was stirred for 12 h at 100° C. The reaction mixture was cooled and concentrated under reduced pressure and diluted using saturated ammonium chloride solution, which was extracted with dichloromethane. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄and evaporated under reduced pressure to obtain the crude compound, which was purified by column chromatography using 25% ethyl acetate in hexane to afford 4-(tert-butyl)-N-(2-(3-cyanobenzyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide as a white solid (59; 0.08 g, 31% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.88 (bs, 1H), 7.94-7.92 (d, J=7.2 Hz, 2H), 7.81-7.74 (m, 3H), 7.64-7.54 (m, 6H), 4.77 (s, 2H), 1.26 (s, 9H). MS (M−1): 472.10 (LCMS purity 98.28%, Rt=6.77 min) (1).
The following compounds were also prepared using a similar method and the appropriate sulfonyl chloride in the final step:


		LCMS
Cpd	Structure	(M − 1)	Purity (LCMS)	¹H NMR

60		484.06	98.76%, Rt = 5.90 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.42 (bs, 1H), 8.16-8.14 (d, J = 8 Hz, 2H), 7.98-7.96 (d, J = 8.0 Hz, 2H), 7.92 (s, 1H), 7.76-7.74 (d, J = 6.4 Hz 2H), 7.63-7.60 (m, 2H), 7.57-7.52 (m, 2H), 4.75 (s, 2H).

61		500.15	98.23% Rt = 6.18 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.22 (bs, 1H), 8.08-8.06 (d, J = 8.8 Hz, 2H), 7.78-7.73 (m, 3H), 7.62-7.52 (m, 6H), 4.75 (s, 2 H).

62		502.09	99.15% Rt = 5.85 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.53 (bs, 1H), 8.34-8.33 (d, J = 5.6 Hz, 1H), 8.27-8.26 (m, 1H), 7.78-7.68 (m, 4H), 7.65-7.52 (m, 4H), 4.74 (s, 2 H).

63		434.12	99.65% Rt = 5.92 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.07 (bs, 1H), 8.02-7.99 (m, 2H), 7.78-7.73 (m, 3H), 7.61-7.52 (m, 4H), 7.43-7.39 (t, J = 8.4 Hz, 2H), 4.76 (s, 2 H).

Example 9

Synthesis of Compound 64 [4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(pyridin-3-ylmethyl) isoindolin-4-yl)benzenesulfonamide]; Compound 65 [3-((4-(4-(tert-butyl)phenylsulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)methyl)pyridine 1-oxide]; and Compounds 66-81

Synthesis of XXXI:

To a stirred solution of compound XXV (5.0 g, 21.1 mmol) in acetic acid (44 mL) was added pyridin-3-ylmethanamine (VI, 7.2 g, 66 mmol) and the reaction mixture heated at 100° C. for 18 h. The reaction mixture was cooled to room temperature and the acetic acid removed under reduced pressure to obtain the crude product which was washed with ethyl acetate to afford 4-chloro-7-nitro-2-(pyridin-3-ylmethyl)isoindoline-1,3-dione as a yellow solid (XXXI; 2 g, 28% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.80 (s, 1H), 8.72-8.69 (m, 1H), 8.29-8.27 (d, J=8.4 Hz, 1H), 8.23 (m, 1H), 8.13-8.10 (d, J=8.4 Hz, 1H), 7.74 (m, 1H), 4.91 (s, 2H). MS (M+1): 317.8 (LCMS Purity 94.26%).

Synthesis of XXXII:

To a solution of compound XXXI (2 g, 6 mmol) in acetic acid (30 mL) was added portion wise iron powder (0.5 g, 18 mmol). The reaction mixture was stirred for 5 h at room temperature. The reaction mixture was concentrated under reduced pressure. The crude mass was neutralized with sodium bicarbonate solution with the resulting aqueous layer being extracted with ethyl acetate. The organic solvent was dried, filtered and evaporated under reduced pressure to obtain the crude compound. The crude material was further purified by trituration using acetonitrile and ethyl acetate to afford compound 4-amino-7-chloro-2-(pyridin-3-ylmethyl)isoindoline-1,3-dione as a brown solid (XXXII; 1.2 g, 70% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.55 (s, 1H), 8.48 (m, 1H), 7.70-7.68 (d, J=7.6 Hz, 1H), 7.42-7.40 (d, J=8.4 Hz, 1H), 7.35 (m, 1H), 7.0-6.98 (d, J=8.8 Hz, 1H), 6.63 (bs, 2H), 4.72 (s, 2H). MS (M+1): 288.12.

Synthesis of 64; 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(pyridin-3-ylmethyl)isoindolin-4-yl)benzenesulfonamide

A mixture of compound XXXII (400 mg, 1.39 mmol) and pyridine (5 mL) was cooled to 0° C. and 4-tert-butylbenzenesulfonyl chloride (V, 0.82 g, 3.53 mmol) was added. The reaction mixture was stirred for 12 h at 80° C. The reaction mixture was concentrated and to the resultant residue, water was added and extracted with dichloromethane. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and then evaporated under reduced pressure to obtain the crude compound which was purified by column chromatography using 2% methanol in dichloromethane to afford 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(pyridin-3-ylmethyl)isoindolin-4-yl)benzenesulfonamide as a white solid (64; 0.08 g, 11.8% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.92 (bs, 1H), 8.57 (s, 1H), 8.50 (s, 1H), 7.92-7.90 (d, J=8 Hz, 2H), 7.75-7.73 (d, J=9.2 Hz, 2H), 7.62-7.58 (m, 3H), 7.38-7.35 (m, 1H), 4.74 (s, 2H), 1.26 (s, 9H). MS (M+1): 484.10. (LCMS purity 97.90%, Rt=6.38 min(1)).

Synthesis of 65; 3-((4-(4-(tert-butyl)phenylsulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)methyl)pyridine 1-oxide

To a stirred solution of 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(pyridin-3-ylmethyl)isoindolin-4-yl)benzenesulfonamide (64; 0.065 g, 0.14 mmol) in dichloromethane (5 mL), was added meta-chloroperoxybenzoic acid (0.04 g, 0.14 mmol). The reaction was stirred at RT for 8 h whereupon the solvent was concentrated under reduced pressure and the residue diluted with water. The aqueous layer was extracted with dichloromethane. The combined organic layers were washed sequentially with sodium bicarbonate and brine, then dried over anhydrous Na₂SO₄, filtered and concentrated under vacuum to leave the crude compound which was purified by column chromatography using 2% methanol in dichloromethane to afford 3-((4-(4-(tert-butyl)phenylsulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)methyl)pyridine 1-oxide (65; 0.012 g, 14% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.93 (bs, 1H), 8.26 (s, 1H), 8.14-8.12 (d, J=6 Hz, 1H), 7.93-7.91 (d, J=8 Hz, 2H), 7.75-7.73 (d, J=8.4 Hz, 1H), 7.63-7.58 (m, 3H), 7.38-7.34 (t, J=6.8 Hz, 1H), 7.29-7.27 (d, J=9.2 Hz, 1H), 4.69 (s, 2H), 1.26 (s, 9H). MS (M+1): 500.12. (LCMS purity 98.73%, Rt=5.47 min(1)).
The following compounds were prepared in a similar manner and using the appropriate sulfonyl chloride in the penultimate step. This resulted either in the final pyridine compounds, which if required could also be converted into the corresponding pyridine N-oxides using mCPBA:


		LCMS	Purity
CPD.	Structure	(M + 1)	(LCMS)	¹H NMR

66		495.92	99.53% Rt = 5.29 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.48 (bs, 1H), 8.55 (s, 1H), 8.49- 8.48 (d, J = 3.6 Hz, 1H), 8.13-8.11 (d, J = 8.4 Hz, 2H), 7.97-7.95 (d, J = 8.4 Hz, 2H), 7.76-7.31 (d, J = 9.2 Hz, 1H), 7.69-7.67 (d, J = 8 Hz, 1H), 7.55-7.53 (d, J = 8.8 Hz, 1H), 7.36-7.33 (m, 1H), 4.71 (s, 2H).

67		511.92	98.75% Rt = 5.48 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.30 (bs, 1H), 8.59 (s, 1H), 8.53- 8.52 (d, J = 4.4 Hz, 1H), 8.07-8.05 (d, J = 8.4 Hz, 2H), 7.77-7.75 (d, J = 8.4 Hz, 2H), 7.58-7.55 (m, 3H), 7.43-7.40 (m, 1H), 4.74 (s, 2H).

68		494.12	99.65% Rt = 5.38 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.10 (bs, 1H), 8.54 (s, 1H), 8.49- 8.48 (d, J = 3.6 Hz, 1H), 8.01-7.99 (d, J = 8.8 Hz, 2H), 7.75-7.68 (m, 2H), 7.57-7.55 (d, J = 8.8 Hz, 1H), 7.39-7.21 (m, 4H), 4.73 (s, 2H).

69		512.14	96.68% Rt = 4.76 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 8.19 (s, 1H), 8.12-8.11 (d, J = 6 Hz, 1H), 7.99-7.97 (d, J = 8 Hz, 2H), 7.78-7.76 (d, J = 8.4 Hz, 2H), 7.48-7.46 (d, J = 9.2 Hz, 1H), 7.36- 7.32 (m, 1H), 7.25-7.23 (d, J = 9.2 Hz, 1H), 7.20-7.18 (d, J = 8 Hz, 1H), 4.62 (s, 2H).

70		528.13	99.02% Rt = 4.89 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 8.21 (s, 1H), 8.13-8.11 (d, J = 6 Hz, 1H), 7.97-7.95 (d, J = 8.4 Hz, 2H), 7.51-7.45 (m, 4H), 7.37-7.33 (m, 1H), 7.22-7.20 (d, J = 7.6 Hz, 1H), 4.64 (s, 2H).

71		530.14	99.40%, Rt = 5.42 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.69 (bs, 1H), 8.55 (s, 1H), 8.50- 8.49 (d, J = 3.2 Hz, 1H), 8.36 (s, 1H), 8.17-8.15 (d, J = 8 Hz, 1H), 7.93-7.91 (d, J = 8 Hz 1H), 7.74- 7.72 (d, J = 8 Hz, 1H) 7.68-7.66 (d, J = 8 Hz, 1H), 7.55-7.53 (d, J = 8 Hz, 1H), 7.38 (t, J = 8 Hz, 1H), 4.71 (s 2H).

72		514.23	99.24%, Rt = 5.27 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.53 (bs, 1H), 8.54 (s, 1H), 8.50- 8.49 (d, J = 4.0 Hz, 1H), 8.34-8.33 (d, J = 6.0 Hz, 1H), 8.26-8.24 (m, 1H), 7.75-7.66 (m, 3H), 7.57-7.55 (d, J = 8.8 Hz, 1H), 7.37-7.34 (m, 1H), 4.71 (s, 2H).

73		456.19 (M − 1)	98.76%, Rt = 5.46 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 9.80 (bs, 1H), 8.55-8.49 (m, 2H), 7.89-7.87 (m, 2H), 7.73-7.68 (m, 2H), 7.59-7.57 (d, J = 8.8 Hz, 1H), 7.36-7.35 (d, J = 4.8 Hz, 1H), 7.09- 7.07 (d, J = 8.0 Hz, 2H), 4.73 (s, 2H), 3.81 (s, 3H).

74		530.30	97.00%, Rt = 4.84 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.57 (bs, 1H), 8.24-8.21 (m, 3H), 8.13-8.12 (d, J = 6.4 Hz, 1H), 7.63 (t, J = 9.6 Hz, 1H), 7.53-7.51 (m, 2H), 7.34 (t, J = 7.2 Hz, 1H) 7.21- 7.19 (d, J = 8.4 Hz, 1H), 4.65 (s, 2H).

75		546.30	98.08%, Rt = 5.01 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 8.19 (m, 2H), 8.12-8.11 (d, J = 5.6, 1H), 8.05-8.03 (d, J = 8.0 Hz, 1H), 7.78-7.76 (d, J = 8 Hz, 1H), 7.50- 7.48 (d, J = 8.8 Hz, 1H), 7.36-7.27 (m, 2H) 7.19-7.18 (d, J = 7.2 Hz, 1H), 4.63 (s, 2H).

76		496.04	98.75%, Rt = 5.21 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.62 (bs, 1H), 8.52-8.50 (m, 2H), 8.24 (s, 1H), 8.17-8.15 (d, J = 8.4 Hz, 1H), 8.03-8.01 (d, J = 7.6 Hz, 1H), 7.81-7.74 (m, 2H), 7.66-7.64 (d, J = 7.2 Hz, 1H), 7.56-7.54 (d, J = 8.8 Hz, 1H), 7.38-7.35 (m, 1H), 4.70 (s, 2H).

77		495.08	98.75%, Rt = 4.83 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.18 (bs, 1H), 8.56 (s, 1H), 8.53 (s, 1H), 8.45-8.44 (d, J = 8.4 Hz, 1H), 8.01-7.99 (d, J = 8.4 Hz, 2H), 7.91-7.89 (m, 3H), 7.76-7.73 (d, J = 8.8 Hz, 1H), 7.66-7.64 (d, J = 8.0 Hz, 1H), 7.59-7.56 (d, J = 8.8 Hz, 1H), 7.30-7.27 (m, 1H), 4.71 (s, 2H).

78		510.09 (M − 1)	97.57%, Rt = 4.77 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.55 (bs, 1H), 8.27 (s, 1H), 8.21- 8.14 (m, 3H), 8.06-8.04 (d, J = 8.4 Hz, 1H), 7.85-7.81 (t, J = 7.6 Hz, 1H), 7.77-7.75 (d, J = 8.8 Hz, 1H), 7.56-7.54 (d, J = 8.8 Hz, 1H), 7.39- 7.35 (t, J = 7.6 Hz, 1H), 7.23-7.21 (d, J = 7.2 Hz, 1H), 4.66 (s, 2H).

79		495.05	99.37%, Rt = 5.45 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.18 (bs, 1H), 8.56 (s, 1H), 8.49 (s, 1H), 8.04-8.02 (d, J = 8.4 Hz, 2H), 7.75-7.71 (m, 4H), 7.58-7.56 (d, J = 8.8 Hz, 1H), 7.37-7.34 (m, 1H), 4.73 (s, 2H), 1.68 (s, 6H).

80		470.38	99.50%, Rt = 6.11 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 9.92 (bs, 1H), 8.55 (s, 1H), 8.49- 8.48 (d, J = 4.4 Hz, 1H), 7.90-7.88 (d, J = 8.0 Hz, 2H), 7.74-7.70 (m, 2H), 7.59-7.57 (d, J = 9.2 Hz, 1H), 7.47-7.45 (m, 2H), 7.37-7.34 (m, 1H), 4.73 (s, 2H), 2.98-2.90 (m, 1H), 1.18-1.16 (d, J = 6.8 Hz, 6H).

81		511.38	99.26%, Rt = 4.52 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.15 (bs, 1H), 8.26 (s, 1H), 8.14- 8.12 (d, J = 6.4 Hz, 1H), 8.06-8.04 (d, J = 8.8 Hz, 2H), 7.76-7.73 (m, 3H), 7.58-7.56 (d, J = 8.8 Hz, 1H), 7.38-7.35 (t, J = 8.0 Hz, 1H), 7.28- 7.27 (d, J = 7.6 Hz, 1H), 4.69 (s, 2H), 1.68 (s, 6H).

Example 10

Synthesis of Compound 82 [4-(tert-butyl)-N-(7-cyano-1,3-dioxo-2-(pyridin-3-ylmethyl)isoindolin-4-yl)benzenesulfonamide]

To a stirred solution of 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(pyridin-3-ylmethyl)isoindolin-4-yl)benzenesulfonamide (64; 0.15 g, 0.31 mmol) in dimethylacetamide (4 mL) was added zinc cyanide (0.073 g, 62 mmol) under nitrogen followed by addition of 1,1′-Bis (diphenylphosphino)ferrocene (0.034 g, 0.062 mmol) and Pd2(dba)3 (0.43 g, 0.046 mmol). To the reaction mixture was added zinc dust (0.005 g) and the reaction mixture was purged under nitrogen for 30 minutes. The reaction was heated at 120° C. for 2 h in microwave. The reaction mixture was cooled, filtered and concentrated under reduced pressure. The crude residue was purified by column chromatography using 4% methanol in dichloromethane to afford 4-(tert-butyl)-N-(7-cyano-1,3-dioxo-2-(pyridin-3-ylmethyl)isoindolin-4-yl)benzenesulfonamide (82; 0.02 g, 13.6% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.70 (s, 1H), 8.61-8.60 (d, J=4.4 Hz, 1H), 8.11-8.09 (d, J=8.8 Hz, 1H), 8.03-7.99 (m, 3H), 7.70-7.64 (m, 3H), 7.58-7.55 (m, 1H), 4.84 (s, 2H), 1.27 (s, 9H). MS (M+1): 475.46. (LCMS purity 99.77%, Rt=5.15 min) (1).

Example 11

Synthesis of Compound 83 [4-(tert-butyl)-N-(7-chloro-2-(5-chloropyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide] and Compounds 84-89

Synthesis of XXXIV:

To a stirred solution of compound XXV (3.0 g, 13.2 mmol) in acetic acid (26 mL) was added 5-chloropyridin-3-amine (XXXIII, 5 g, 39 mmol) and the reaction mixture heated at 100° C. for 18 h. The reaction mixture was cooled to room temperature and the acetic acid removed under reduced pressure to obtain the crude product which was washed with ethanol to afford 4-chloro-2-(5-chloropyridin-3-yl)-7-nitroisoindoline-1,3-dione as an off white solid (XXXIV; 4 g, 89% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.78 (s, 1H), 8.64 (d, J=1.6 Hz, 1H), 8.40-8.38 (d, J=8.8 Hz, 1H), 8.25-8.22 (d, J=9.2 Hz, 1H), 8.06 (s, 1H).

Synthesis of XXXV:

To a solution of compound XXXIV (4 g, 11.8 mmol) in acetic acid (200 mL) was added iron powder (1.4 g, 23 mmol) in small portions. The reaction mixture was stirred for 5 h at room temperature and then filtered through a celite bed and concentrated under reduced pressure. The crude mass was neutralized by sodium bicarbonate solution. The aqueous layer was extracted with ethyl acetate, which was dried (anhydrous Na₂SO₄), filtered and evaporated under reduced pressure to obtain the crude compound. This material was further purified using trituration with acetonitrile and ethyl acetate to afford the compound 4-amino-7-chloro-2-(5-chloropyridin-3-yl)isoindoline-1,3-dione as a yellow solid (XXXV; 3 g, 81% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.69-8.66 (m, 2H), 8.10 (s, 1H), 7.52-7.50 (d, J=8.8 Hz, 1H), 7.09-7.07 (d, J=9.2 Hz, 1H), 6.78 (bs, 2H). MS (M+1): 307.98 (LCMS Purity 97.9%).

Synthesis of 83; 4-(tert-butyl)-N-(7-chloro-2-(5-chloropyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide

A mixture of compound XXXV (300 mg, 0.97 mmol) and pyridine (3 mL) was cooled to 0° C. and added 4-tert-butylbenzenesulfonyl chloride (V, 0.95 g, 2.9 mmol). The reaction mixture was stirred for 12 h at 100° C. The reaction mixture was concentrated and diluted with water. The reaction mixture was extracted with ethyl acetate. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound. To the crude compound was added TBAF in THF solution (4 mL) and continued the stirring for 1 h at room temperature. The reaction mixture was concentrated and diluted with ethyl acetate. Washed an organic layer with water, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound which was purified by column chromatography using 40% ethyl acetate in hexane to afford the title compound, 4-(tert-butyl)-N-(7-chloro-2-(5-chloropyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide as a yellow solid (83; 0.11 g, 23% yield). ¹H NMR (400 MHz, DMSO-d6): δ 10.06 (bs, 1H), 8.74 (d, J=2.0 Hz, 1H), 8.61 (d, J=1.6 Hz, 1H), 8.04 (m, 1H), 7.94-7.91 (d, J=8.4 Hz, 2H), 7.87-7.84 (d, J=8.8 Hz, 1H), 7.71-7.69 (d, J=8.8 Hz, 1H), 7.65-7.63 (d, J=8.8 Hz, 2H), 1.27 (s, 9H). MS (M+1): 504.07. (LCMS purity 97.67%, Rt=6.34 min) (1).
The following compounds were also prepared using a similar method and the appropriate sulfonyl chloride in the final step:


		LCMS	Purity
CPD	Structure	(M + 1)	(LCMS)	¹H NMR

84		533.83	95.92%, Rt = 6.82 min (2)	¹H NMR (400 MHz, DMSO-d6): δ 10.78 (bs, 1H), 8.75-8.74 (d, J = 2 Hz, 1H), 8.60-8.59 (d, J = 2.0 Hz, 1H), 8.45-8.44 (d, J = 4.4 Hz, 1H), 8.33-8.31 (m, 1H), 8.01-8.00 (m, 1H), 7.85-7.83 (d, J = 8.8 Hz, 1H), 7.80-7.76 (t, J = 9.6 Hz, 1H), 7.65- 7.63 (d, J = 8.8 Hz, 1H).

85		514.11 (M − 1)	99.16%, Rt = 5.78 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.63 (bs, 1H), 8.74-8.73 (d, J = 2.4 Hz, 1H), 8.58-8.57 (d, J = 1.6 Hz, 1H), 8.15-8.13 (d, J = 8 Hz, 2H), 8.00-7.99 (m, 3H), 7.87-7.84 (d, J = 9.2 Hz, 1H), 7.64-7.62 (d, J = 8.8 Hz, 1H).

86		530.10 (M − 1)	99.04%, Rt = 5.91 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.45 (bs, 1H), 8.74 (s, 1H), 8.59 (s, 1 H), 8.10-8.08 (d, J = 8.4 Hz, 2H), 8.01 (s, 1H), 7.86-7.84 (d, J = 8 Hz, 1H), 7.66-7.60 (m, 3H).

87		512.05 (M − 1)	99.48%, Rt = 5.63 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.25 (bs, 1H), 8.75-8.74 (d, J = 2.4 Hz, 1H), 8.62-8.61 (d, J = 2 Hz, 1H), 8.07-8.03 (m, 3H), 7.85-7.83 (d, J = 8.8 Hz, 1H), 7.67-7.59 (m, 1H), 7.41-7.23 (m, 3H).

88		516.17 (M + 1)	95.82%, Rt = 5.56 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.70 (bs, 1H), 8.75-8.74 (d, J = 2.4 Hz, 1H), 8.60-8.59 (d, J = 2 Hz, 1H), 8.39 (s, 1H), 8.28-8.26 (d, J = 8 Hz, 1H), 8.11-8.09 (d, J = 8.4 Hz, 1H), 8.02-8.00 (m, 1H), 7.89-7.83 (m, 2H), 7.64-7.61 (d, J = 9.2 Hz, 1H).

89		464.09 (M − 1)	99.48%, Rt = 5.50 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.27 (bs, 1H), 8.75-8.74 (d, J = 2 Hz, 1H), 8.62-8.61 (d, J = 1.6 Hz, 1H), 8.08-8.03 (m, 3H), 7.85-7.83 (d, J = 8.8 Hz, 1H), 7.66-7.64 (d, J = 8.8 Hz, 1H), 7.49-7.45 (t, J = 8.8 Hz, 2H).

Example 12

Synthesis of Compound 90 [4-(tert-butyl)-N-(7-chloro-2-(5-methoxypyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide]; Compound 91 [3-(4-(4-(tert-butyl)phenylsulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)-5-methoxypyridine 1-oxide] and Compounds 92-103

Synthesis of XXXVII:

To a stirred solution of compound XXV (2.0 g, 8.8 mmol) in acetic acid (10 mL) was added 5-methoxypyridin-3-amine (XXXVI, 2.7 g, 22 mmol) and the reaction mixture heated at 120° C. for 18 h. The reaction mixture was cooled to room temperature and the acetic acid removed under reduced pressure to obtain the crude product which was washed with ethanol to afford 4-chloro-2-(5-methoxypyridin-3-yl)-7-nitroisoindoline-1,3-dione as a brown solid (XXXVII; 2.5 g crude). MS (M+1): 333.84. The crude was carried forward to next step without purification.

Synthesis of XXXVIII:

To a solution of compound XXXVII (2.5 g, crude) in acetic acid (10 mL) was added iron powder (2 g) in small portions. The reaction mixture was stirred for 5 h at room temperature. The reaction mixture was filtered through a celite bed and concentrated under reduced pressure. The crude material was neutralized using aqueous sodium bicarbonate solution. The aqueous layer was extracted with ethyl acetate which was dried (anhydrous Na₂SO₄), filtered and evaporated under reduced pressure to obtain the crude compound. This material was further purified by trituration using acetonitrile and ethyl acetate as solvents to afford compound 4-amino-7-chloro-2-(5-methoxypyridin-3-yl)isoindoline-1,3-dione as a off white solid (XXXVIII; 2 g, 88% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.35 (s, 1H), 8.25 (s, 1H), 7.53-7.48 (m, 2H), 7.08 (m, 1H), 6.75 (bs, 2H), 3.85 (s, 3H). MS (M+1): 304.10.

Synthesis of 90; 4-(tert-butyl)-N-(7-chloro-2-(5-methoxypyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide

A mixture of compound XXXVIII (200 mg, 0.66 mmol) and pyridine (3 mL) was cooled to 0° C. and 4-tert-butylbenzenesulfonyl chloride (V, 0.46 g, 1.98 mmol) was added together with catalytic DMAP (0.040 g, 0.33 mmol). The reaction mixture was stirred for 12 h at 80° C. and then concentrated under vacuum and diluted with water. The aqueous layer was extracted with ethyl acetate which was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound. To the crude compound was added 1M TBAF in THF solution (2 mL) and the stirring continued for 1 h at room temperature. The reaction mixture was concentrated and diluted with ethyl acetate. This was washed with water, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound which was purified by column chromatography using 20% ethyl acetate in hexane to the title compound 4-(tert-butyl)-N-(7-chloro-2-(5-methoxypyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide as a yellow solid (90; 0.065 g, 19.7% yield). ¹H NMR (400 MHz, DMSO-d6): δ 10.03 (bs, 1H), 8.39-8.38 (d, J=2.4 Hz, 1H), 8.22-8.21 (d, J=1.6 Hz, 1H), 7.94-7.92 (d, J=8.4 Hz, 2H), 7.84-7.82 (d, J=9.2 Hz, 1H), 7.69-7.63 (m, 3H), 7.50-7.48 (m, 1H), 3.85 (s, 3H), 1.25 (s, 9H). MS (M−1): 498.20. (LCMS purity 98.37%, Rt=6.20 min) (1).

Synthesis of 91; 3-(4-(4-(tert-butyl)phenylsulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)-5-methoxypyridine 1-oxide

To a stirred solution of 4-(tert-butyl)-N-(7-chloro-2-(5-methoxypyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide (90, 0.1 g, 0.2 mmol) in dichloromethane (4 mL), was added m-chloroperoxybenzoic acid (0.035 g, 0.14 mmol). The reaction was stirred at RT for 5 h whereupon the solvent was concentrated under reduced pressure and the residue diluted with water. The aqueous layer was extracted with dichloromethane. The organic layer was washed sequentially with sodium bicarbonate and brine, then dried over Na₂SO₄, filtered and concentrated under vacuum to leave the crude compound which was purified by column chromatography using 3% methanol in dichloromethane to afford the title compound 3-(4-(4-(tert-butyl)phenylsulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)-5-methoxypyridine 1-oxide (91; 0.05 g, 48% yield). ¹H NMR (400 MHz, DMSO-d6): δ 10.08 (bs, 1H), 8.20 (s, 1H), 8.01 (s, 1H), 7.94-7.92 (m, 2H), 7.86-7.82 (m, 1H), 7.69-7.63 (m, 3H), 7.15 (s, 1H), 3.85 (s, 3H), 1.27 (s, 9H). MS (M+1): 516.07. (LCMS purity 99.19%, Rt=5.34 min) (1).
The following compounds except 103 were prepared in a similar manner and using the appropriate sulfonyl chloride in the penultimate step. This resulted either in the final pyridine compounds, which if required could also be converted into the corresponding pyridine N-oxides using mCPBA. Compound 103 was prepared using 5-ethoxypyridin-3-amine in the first step described:


		LCMS	Purity
CPD	Structure	(M + 1)	(LCMS)	¹H NMR

92	510.08 (M − 1)	99.35%, Rt = 5.40 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.53 (bs, 1H), 8.39- 8.38 (d, J = 2.4 Hz, 1H), 8.18-8.17 (m, 3H), 8.02-8.00 (d, J = 8.4 Hz, 2H), 7.85-7.83 (d, J = 8.8 Hz, 1H), 7.63-7.61 (d, J = 8.8 Hz, 1H), 7.46-7.45 (s, 1H), 3.85 (s, 3H).

93	544.08 (M − 1)	99.43%, Rt = 5.58 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.79 (bs, 1H), 8.43 (s, 1H), 8.39-8.38 (d, J = 2.4 Hz, 1H), 8.22-8.19 (m, 2H), 8.00- 7.98 (d, J = 8.4 Hz, 1H), 7.84-7.81 (d, J = 8.8 Hz, 1H), 7.63-7.61 (d, J = 8.8 Hz, 1H), 7.46-7.45 (m, 1H), 3.85 (s, 3H).

94	528.15	99.47%, Rt = 5.56 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.39 (bs, 1H), 8.39- 8.38 (d, J = 2.8 Hz, 1H), 8.20-8.19 (d, J = 1.6 Hz, 1H), 8.11-8.09 (d, J = 8.8 Hz, 2H), 7.85-7.83 (d, J = 8.8 Hz, 1H), 7.65-7.60 (m, 3H), 7.47-7.46 (d, J = 2 Hz, 1H), 3.85 (s, 3H).

95	530.18	98.00%, Rt = 5.39 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.69 (bs, 1H), 8.44- 8.42 (d, J = 5.2 Hz, 1H), 8.39-8.38 (d, J = 2.8 Hz, 1H), 8.33-8.32 (d, J = 2.8 Hz, 1H), 8.20-8.19 (d, J = 2 Hz, 1H), 7.83-7.76 (m, 2H), 7.64-7.62 (d, J = 8.8 Hz, 1H), 7.47-7.46 (d, J = 2.4 Hz, 1H), 3.85 (s, 3H).

96	511.06	96.98%, Rt = 4.88 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.24 (bs, 1H), 8.56 (s, 1H), 8.38 (d, J = 2.8 Hz, 1H), 8.20 (d, J = 1.6 Hz, 1H), 8.07-8.05 (d, J = 8.4 Hz, 2H), 7.96-7.92 (m, 3H), 7.83-7.81 (d, J = 8.0 Hz, 1H), 7.66-7.64 (d, J = 8.8 Hz, 1H), 7.46 (m, 1H), 3.84 (s, 3H).

97	510.06	98.99%, Rt = 5.33 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.20 (bs, 1H), 8.39 (d, J = 2.8 Hz, 1H), 8.22 (s, 1H), 8.07-8.05 (d, J = 8.8 Hz, 2H), 7.84-7.81 (d, J = 8.8 Hz, 1H), 7.66-7.63 (d, J = 8.8 Hz, 1H), 7.59-7.22 (m, 4H), 3.85 (s, 3H).

98	545.99	99.31%, Rt 5.05 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.76 (bs, 1H), 8.38- 8.30 (m, 2H), 8.18 (s, 1H), 8.01 (s, 1H), 7.75-7.71 (m, 2H), 7.61-7.59 (m, 1H), 7.14 (s, 1H), 3.85 (s, 3H).

99	561.97	99.42%, Rt = 5.23 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.75 (bs, 1H), 8.35 (s, 1H), 8.16 (bs, 2H), 8.02 (s, 1H), 7.92-7.90 (d, J = 8.0 Hz, 1H), 7.66-7.57 (m, 2H), 7.15 (s, 1H), 3.85 (s, 3H).

100	526.01	99.59%, Rt = 4.88 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.27 (bs, 1H), 8.18 (s, 1H), 8.03-8.02 (d, J = 5.6 Hz, 3H), 7.77-7.72 (m, 1H), 7.63- 7.61 (d, J = 8.8 Hz, 1H), 7.37-7.34 (m, 2H), 7.22-7.09 (m, 2H), 3.85 (s, 3H).

101	528.03	98.14%, Rt = 5.96 min (2)	¹H NMR (400 MHz, DMSO- d6): δ 10.75 (bs, 1H), 8.11 (s, 1H), 8.05 (s, 1H), 8.01-7.99 (d, J = 8.4 Hz, 2H), 7.80-7.78 (d, J = 8.4 Hz, 2H), 7.55-7.53 (d, J = 7.2 Hz, 1H), 7.33-7.30 (d, J = 9.2 Hz, 1H), 7.19 (s, 1H), 3.84 (s, 3H).

102	544.04	96.39%, Rt = 6.04 min (2)	¹H NMR (400 MHz, DMSO- d6): δ 10.46 (bs, 1H), 8.19 (s, 1H), 8.12-8.10 (d, J = 8.8 Hz, 2H), 8.00 (s, 1H), 7.83-7.81 (d, J = 5.2 Hz, 1H), 7.65-7.60 (m, 3H), 7.13 (s, 1H), 3.85 (s, 3 H).

103	514.47	98.16%, Rt = 6.28 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.02 (bs, 1H), 8.36 (s, 1H), 8.19 (s, 1H), 7.93-7.91 (m, 2H), 7.84-7.82 (d, J = 8.8 Hz, 1H), 7.69-7.63 (m, 3H), 7.46 (s, 1H), 4.15-4.10 (q, J = 6.8 Hz, 2H), 1.38-1.34 (t, J = 6.8 Hz, 3H), 1.27 (s, 9H).

Example 13

Synthesis of Compound 104 [4-(tert-butyl)-N-(7-chloro-2-(5-methylpyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide] and Compound 105

Synthesis of XL:

To a stirred solution of compound XXV (2 g, 8.81 mmol) in acetic acid (18 mL) was added 5-methylpyridin-3-amine (XXXIX, 1.43 g, 13.2 mmol). The reaction mixture was heated at 120° C. for 12 h. On cooling to room temperature, the acetic acid was removed under reduced pressure to obtain the crude product which was washed with ethanol to afford 4-chloro-2-(5-methylpyridin-3-yl)-7-nitroisoindoline-1,3-dione (XL, 1.8 g, 64.5%). ¹H NMR (400 MHz, DMSO-d6): δ 8.63 (s, 1H), 8.52 (m, 1H), 8.20-8.16 (m, 1H), 7.86-7.84 (d, J=8.4 Hz, 1H), 7.70 (m, 1H), 2.3 (s, 3H).

Synthesis of XLI:

To a solution of compound XL (1.8 g, 5.67 mmol) in acetic acid (50 mL) was added iron powder (1 g) in small portions. The reaction mixture was stirred for 12 h at room temperature and then filtered through a celite bed and concentrated under reduced pressure. The crude material was neutralized by addition of sodium bicarbonate solution. The aqueous layer was extracted with ethyl acetate, which was dried (anhydrous Na₂SO₄), filtered and evaporated under reduced pressure to afford compound 4-amino-7-chloro-2-(5-methylpyridin-3-yl)isoindoline-1,3-dione as an off white solid (XLI; 1.4 g, 87.5% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.53-8.44 (m, 2H), 7.70 (s, 1H), 7.50-7.47 (d, J=8.8 Hz, 1H), 7.07-7.05 (d, J=8.8 Hz, 1H), 6.73 (bs, 2H), 2.36 (s, 3H).

Synthesis of 104; 4-(tert-butyl)-N-(7-chloro-2-(5-methylpyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide and Compound 105

A mixture of compound XLI (0.3 g, 1.05 mmol) and pyridine (6 mL) was cooled to 0° C. and 4-tert-butylbenzenesulfonyl chloride (V, 0.29 g, 1.25 mmol) was added followed by a catalytic quantity of DMAP (0.034 g, 0.42 mmol). The reaction mixture was heated for 15 h at 80° C. The reaction mixture was concentrated and diluted with water. The reaction mixture was extracted with ethyl acetate. An organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound. The crude compound was purified by column chromatography using 70% ethyl acetate in hexane to afford 4-(tert-butyl)-N-(7-chloro-2-(5-methylpyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide as an off white solid (104; 0.2 g, 39.8% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.97 (bs, 1H), 8.49 (s, 1H), 8.40 (s, 1H), 7.93-7.91 (d, J=8.4 Hz, 2H), 7.84-7.82 (d, J=9.2 Hz, 1H), 7.69-7.63 (m, 4H), 2.37 (s, 3H), 1.27 (s, 9H). MS (M+1): 484.20. (LCMS purity 96.68%, Rt=6.22 min (1)).
The following compound was also prepared using a similar method and the appropriate sulfonyl chloride in the final step:


		LCMS	Purity
CPD	Structure	(M + 1)	(LCMS)	1HNMR

105		470.38	98.96%, Rt = 6.05 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 9.97 (bs, 1H), 8.49 (s, 1H), 8.41 (d, J = 2.0 Hz, 1H), 7.93-7.91 (d, J = 8.4 Hz, 2H), 7.84-7.81 (d, J = 8.8 Hz, 1H), 7.69-7.67 (d, J = 8.8 Hz, 2H), 7.51-7.48 (d, J = 8.4 Hz, 2H), 3.0- 2.94 (m, 1H), 2.32 (s, 3H), 1.19-1.18 (d, J = 6.8 Hz, 6H).

Example 14

Synthesis of Compound 106 [4-(tert-butyl)-N-(7-chloro-2-(5-fluoropyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide]

Synthesis of XLIII:

To a stirred solution of compound XXV (0.4 g, 1.7 mmol) in acetic acid (4 mL) was added 5-fluoropyridin-3-amine (XLII, 0.39 g, 3.5 mmol). The reaction mixture was heated at 120° C. for 12 h. The reaction mixture was cooled to room temperature and the acetic acid removed under reduced pressure to obtain the crude product which was washed with ethanol to afford 4-chloro-2-(5-fluoropyridin-3-yl)-7-nitroisoindoline-1,3-dione (XLIII, 0.5 g, 41.6%). MS (M+1): 322.0. (LCMS purity 98.04%).

Synthesis of XLIV:

To a solution of compound XLIII (0.5 g, 1.56 mmol) in acetic acid (10 mL) was added iron powder (0.5 g) in small portions. The reaction mixture was stirred for 12 h at room temperature. The reaction mixture was filtered through a celite bed and concentrated under reduced pressure. The crude mass was neutralized using aqueous sodium bicarbonate solution, whereupon the aqueous layer was extracted with ethyl acetate which was dried (anhydrous Na₂SO₄), filtered and evaporated under reduced pressure to obtain the crude compound 4-amino-7-chloro-2-(5-fluoropyridin-3-yl)isoindoline-1,3-dione as a off white solid (XLIV; 0.35 g, 76% yield). MS (M+1): 292.05 which was used without further purification.

Synthesis of 106; 4-(tert-butyl)-N-(7-chloro-2-(5-fluoropyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide

A mixture of compound XLIV (0.35 g, 1.19 mmol) and pyridine (3 mL) was cooled to 0° C. and 4-tert-butylbenzenesulfonyl chloride (V, 0.56 g, 2.38 mmol) was added. The reaction mixture was stirred for 12 h at 100° C. The reaction mixture was concentrated, diluted with water and extracted with ethyl acetate. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and evaporated under reduced pressure to obtain the crude compound. To the crude compound was added 1M TBAF in THF solution (2.4 mL) and this solution was stirred for 1 h at room temperature. The reaction mixture was concentrated and diluted with ethyl acetate. The organic layer was washed with water, dried over anhydrous Na₂SO₄, filtered and evaporated under reduced pressure to obtain the crude compound which was purified by column chromatography using 50% ethyl acetate in hexane to afford methyl 4-(tert-butyl)-N-(7-chloro-2-(5-fluoropyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide as an off white solid (106; 0.13 g, 22.2% yield). ¹H NMR (400 MHz, DMSO-d6): δ 10.05 (bs, 1H), 8.71 (d, J=2.4 Hz, 1H), 8.54 (s, 1H), 7.94-7.91 (d, J=8.4 Hz, 2H), 7.87-7.84 (m, 2H), 7.71-7.68 (d, J=9.2 Hz, 1H), 7.65-7.63 (d, J=8.4 Hz, 2H), 1.27 (s, 9H). MS (M+1): 488.07. (LCMS purity 97.48%, Rt=4.60 min) (1).

Example 15

Synthesis of Compound 107 [4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(2-(pyridin-3-yl)propan-2-yl)isoindolin-4-yl)benzenesulfonamide]; Compound 108; [3-(2-(4-(4-(tert-butyl)phenylsulfon-amido)-7-chloro-1,3-dioxoisoindolin-2-yl)propan-2-yl)pyridine 1-oxide] and Compounds 109-115

Synthesis of XLVI:

Cerium chloride (35.5 g, 144 mmol) was added to dry THF (250 mL). The reaction mixture was stirred at room temperature for 2 h under a nitrogen atmosphere to allow the cerium chloride to form a suspension in the THF solution. This was cooled to −78° C. and then a 1.6 M methyl lithium solution in THF (48 mL, 144 mmol) was added. The reaction mixture was stirred for 30 minutes maintaining the same temperature and then a solution of 3-cyanopyridine (XLV, 5 g, 48 mmol) in THF (50 mL) was added through a cannula. The reaction mixture was allowed to warm to room temperature and stirring continued for 12 h. The reaction mixture was diluted with a saturated aqueous solution of ammonium acetate solution and the stirring continued for a further 1 h at room temperature. The reaction mixture was filtered through a celite bed, concentrated and diluted with water. The resulting aqueous layer was extracted with ethyl acetate and the organic layer was dried (anhydrous Na₂SO₄), filtered and evaporated under reduced pressure to obtain the crude compound 2-(pyridin-3-yl)propan-2-amine (XLVI; 2.0 g crude). MS (M+1): 137. The crude material was carried forward to the next step without purification.

Synthesis of XLVII:

To a stirred solution of compound XXV (2.0 g, 8.8 mmol) in acetic acid (50 mL) was added 2-(pyridin-3-yl)propan-2-amine (XLVI, 3.6 g, 26 mmol) and the reaction mixture heated at 120° C. for 18 h. This was then cooled to room temperature and the acetic acid removed under reduced pressure to obtain the crude product which was washed with ethanol to afford 4-chloro-7-nitro-2-(2-(pyridin-3-yl)propan-2-yl)isoindoline-1,3-dione as a yellow solid (XLVII; 1.5 g crude). The crude compound carried forward to next step. MS (M+1): 346.04.

Synthesis of XLVIII:

To a solution of compound XLVII (1.5 g, crude) in acetic acid (30 mL) was added iron powder (1.5 g) in small portions. The reaction mixture was stirred for 5 h at room temperature and then filtered through a celite bed and concentrated under reduced pressure. The crude mass was neutralized using aqueous sodium bicarbonate solution resulting in an aqueous layer which was extracted with ethyl acetate. The organic layer was dried (anhydrous Na₂SO₄), filtered and evaporated under reduced pressure to obtain the crude compound. This material was further purified by trituration using acetonitrile and ethyl acetate to afford the compound 4-amino-7-chloro-2-(5-methoxypyridin-3-yl)isoindoline-1,3-dione as a yellow solid (XLVIII; 0.8 g, crude). MS (M+1): 316.

Synthesis of 107; 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(2-(pyridin-3-yl)propan-2-yl)isoindolin-4-yl)benzenesulfonamide

A mixture of compound XLVIII (0.4 g, crude) and pyridine (4 mL) was cooled to 0° C. and 4-tert-butylbenzenesulfonyl chloride (V, 0.88 g, 3.8 mmol) was added followed by a catalytic quantity of DMAP (0.07 g, 0.63 mmol). The reaction mixture was stirred for 12 h at 100° C. whereupon the reaction mixture was concentrated at reduced pressure and diluted with water. The aqueous layer was extracted with ethyl acetate. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound. To the crude compound was added 1M TBAF in THF solution (2 mL) and stirring was continued for 1 h at room temperature. The reaction mixture was concentrated and diluted with ethyl acetate which was with water, dried over anhydrous Na₂SO₄, filtered and evaporated under reduced pressure to obtain the crude compound which was purified by column chromatography using 35% ethyl acetate in hexane to afford the title compound 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(2-(pyridin-3-yl)propan-2-yl)isoindolin-4-yl)benzenesulfonamide as an off white solid (107; 0.065 g, 10% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.80 (bs, 1H), 8.63 (s, 1H), 8.44 (m, 1H), 7.89-7.87 (d, J=8.40 Hz, 2H), 7.81-7.79 (d, J=8.40 Hz, 1H), 7.73-7.70 (d, J=8.40 Hz, 1H), 7.63-7.58 (m, 3H), 7.34-7.31 (m, 1H), 1.89 (s, 6H). 1.27 (s, 9H). MS (M+1): 512.14. (LCMS purity 97.95%, Rt=4.36 min (1)).

Synthesis of 108; 3-(2-(4-(4-(tert-butyl)phenylsulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)propan-2-yl)pyridine 1-oxide

To a stirred solution of 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(2-(pyridin-3-yl)propan-2-yl)isoindolin-4-yl)benzenesulfonamide (107; 0.060 g, 0.11 mmol) in dichloromethane (3 mL), was added metachloroperoxybenzoic acid (0.020 g, 0.11 mmol). The reaction was stirred at room temperature for 8 h whereupon the solvent was concentrated under reduced pressure and the residue diluted with water. The aqueous layer was extracted with dichloromethane. The combined organic layers were washed sequentially with sodium bicarbonate and brine, then dried over Na₂SO₄, filtered and concentrated under vacuum to leave the crude compound which was purified by column chromatography using 2% methanol in dichloromethane to afford 3-(2-(4-(4-(tert-butyl)phenylsulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)propan-2-yl)pyridine 1-oxide (141; 0.020 g, 32% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.81 (bs, 1H), 8.36 (s, 1H), 8.10-8.09 (d, J=5.6 Hz, 1H), 7.91-7.89 (d, J=8.4 Hz, 2H), 7.73-7.71 (d, J=8.8 Hz, 1H), 7.64-7.58 (m, 3H), 7.41-7.32 (m, 2H), 1.85 (s, 6H), 1.27 (s, 9H). MS (M+1): 528.41. (LCMS purity 99.0%, Rt=6.14 min(1)).
The following compounds were prepared in a similar manner and using the appropriate sulfonyl chloride in the penultimate step. This resulted either in the final pyridine compounds, which if required could also be converted into the corresponding pyridine N-oxides using mCPBA:


		LCMS	Purity
CPD	Structure	(M + 1)	(LCMS)	¹H NMR

109		524.06	97.23%, Rt = 6.07 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.31 (bs, 1H), 8.60 (s, 1H), 8.44-8.43 (d, J = 3.6 Hz, 1H), 8.08-8.06 (d, J = 8.4 Hz, 2H), 7.98-7.96 (d, J = 8.40 Hz, 2H). 7.77-7.73 (m, 2H), 7.57-7.54 (d, J = 8.8 Hz, 1H), 7.33-7.30 (m, 1H), 1.85 (s, 6H).

110		558.01	98.91%, Rt = 5.92 min (2)	¹H NMR (400 MHz, DMSO-d6): δ 10.59 (bs, 1H), 8.61 (s, 1H), 8.45-8.44 (d, J = 3.6 Hz, 1H), 8.33 (s, 1H), 8.06-8.04 (d, J = 7.2 Hz, 1H), 7.94-7.92 (d, J = 8.4 Hz, 1H), 7.77-7.73 (m, 2H), 7.57- 7.55 (d, J = 8.8 Hz, 1H), 7.35- 7.32 (m, 1H), 1.86 (s, 6 H).

111		540.38	98.48%, Rt = 6.08 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.15 (bs, 1H), 8.61 (s, 1H), 8.44-8.43 (d, J = 4.4 Hz, 1H), 8.04-8.02 (d, J = 8.8 Hz, 2H), 7.78-7.72 (m, 2 H), 7.59-7.55 (m, 3H), 7.33-7.30 (m, 1H), 1.87 (s, 6H).

112		523.09	97.12%, Rt = 5.52 min (2)	¹H NMR (400 MHz, DMSO-d6): δ 10.00 (bs, 1H), 8.62-(s, 1H), 8.44-8.43 (d, J = 4.0 Hz, 1H), 8.01-7.98 (d, J = 8.4 Hz, 2H), 7.85-7.70 (m, 4H), 7.59-7.57 (d, J = 8.8 Hz, 1H), 7.34-7.31 (m, 1H), 1.89 (s, 6H), 1.69 (s, 6H).

113		522.36	98.02%, Rt = 6.0 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 9.96 (bs, 1H), 8.62 (s, 1H), 8.44-8.43 (d, J = 3.6 Hz, 1H), 7.99-7.97 (d, J = 8.8 Hz, 2H), 7.79-7.77 (d, J = 7.6 Hz, 1H), 7.73-7.71 (d, J = 8.4 Hz, 1H), 7.60-7.55 (m, 1H), 7.41-7.23 (m, 4H), 1.89 (s, 6H).

114		538.26 (M − 1)	99.36%, Rt = 5.18 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.29 (bs, 1H), 8.33 (s, 1H), 8.10-8.08 (m, 3H), 7.99-7.97 (d, J = 8.4 Hz, 2H), 7.75-7.73 (d, J = 8.4 Hz, 1H), 7.57-7.55 (d, J = 8.8 Hz, 1H), 7.36-7.31 (m, 2H), 1.90 (s, 6H).

115		556.30	97.30%, Rt = 5.43 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.13 (bs, 1H), 8.35 (s, 1H), 8.10-8.04 (m, 3H), 7.75-7.72 (d, J = 8.8 Hz, 1H), 7.60-7.55 (m, 3H), 7.38-7.32 (m, 2H), 1.82 (s, 6H).

Example 16

Synthesis of Compound 116 [4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(2-(pyridin-3-yl)ethyl)isoindolin-4-yl)benzenesulfonamide]; and Compound 117 [3-(2-(4-(4-(tert-butyl)phenyl-sulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)ethyl)pyridine 1-oxide]

Synthesis of L:

To a stirred solution of compound XXV (1.2 g, 5.28 mmol) in acetic acid (20 mL) was added 2-(pyridin-3-yl)ethylamine (XLIX, 0.71 g, 5.81 mmol) and the reaction mixture heated at 120° C. for 15 h. The reaction mixture was cooled to room temperature and the acetic acid removed under reduced pressure to obtain the crude product. This was washed with ethanol to afford 4-chloro-7-nitro-2-(2-(pyridin-3-yl)ethyl)isoindoline-1,3-dione as a white solid (L; 1.5 g, 86% yield). The crude compound was carried forward to next step. ¹H NMR (400 MHz, DMSO-d6): δ 8.45 (s, 1H), 8.26-8.24 (d, J=8.8 Hz, 1H), 8.09-8.07 (d, J=8.8 Hz, 1H), 7.71-7.69 (d, J=7.6 Hz, 1H), 7.33-7.30 (m, 1H), 3.83-3.79 (t, J=7.6 Hz, 2H), 2.95-2.91 (t, J=7.6 Hz, 2H). MS (M+1): 332.09 (LCMS Purity 98.15%).

Synthesis of LI:

To a solution of compound L (1 g, 3.01 mmol) in acetic acid (20 mL) was added iron powder (1.5 g) in small portions. The reaction mixture was stirred for 5 h at room temperature. The reaction mixture was filtered through a celite bed and concentrated under reduced pressure. The crude material was neutralized using aqueous sodium bicarbonate solution. The resultant aqueous layer was extracted with ethyl acetate which was dried (anhydrous Na₂SO₄), filtered and evaporated under reduced pressure to obtain the crude compound. The material was further purified by trituration using acetonitrile and ethyl acetate to afford 4-amino-7-chloro-2-(2-(pyridin-3-yl)ethyl)isoindoline-1,3-dione as a yellow solid (LI; 0.75 g, 82.4% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.40-8.38 (m, 2H), 7.64-7.62 (m, 2H), 7.39-7.37 (d, J=8.8 Hz, 1H), 7.31-7.27 (m, 1H), 6.56 (bs, 2H), 3.77-3.74 (t, J=7.2 Hz, 2H), 2.93-2.90 (t, J=6.8 Hz, 2H). MS (M+1): 302.17 (LCMS purity 97.87%).

Synthesis of 116; 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(2-(pyridin-3-yl)ethyl)isoindolin-4-yl)benzenesulfonamide

A mixture of compound LI (1 g, 3.31 mmol) and pyridine (7 mL) was cooled to 0° C. and 4-tert-butylbenzenesulfonyl chloride (V, 2.3 g, 9.89 mmol) was added followed by a catalytic quantity of DMAP (0.040 g, 0.33 mmol). The reaction mixture was stirred for 12 h at 100° C., concentrated and diluted with water. The reaction mixture was extracted with ethyl acetate and the organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and evaporated under reduced pressure to obtain the crude compound. To this material was added 1M TBAF in THF solution (2 mL) and the stirring was continued for 1 h at room temperature. The reaction mixture was concentrated and diluted with ethyl acetate. The organic layer was washed with water, dried over anhydrous Na₂SO₄, filtered and evaporated under reduced pressure to obtain the crude compound which was purified by column chromatography using 20% ethyl acetate in hexane to afford the title compound, 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(2-(pyridin-3-yl)ethyl)isoindolin-4-yl)benzenesulfonamide as a off white solid (116; 0.3 g, 18.75% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.91 (bs, 1H), 8.41-8.37 (m, 2H), 7.89-7.86 (d, J=8.4 Hz, 2H), 7.73-7.71 (d, J=8.8 Hz, 1H), 7.64-7.58 (m, 4H), 7.30-7.29 (d, J=5.2 Hz, 1H), 3.77-3.74 (t, J=6.4 Hz, 2H), 2.90-2.87 (t, J=6.4 Hz, 2H), 1.26 (s, 9H). MS (M+1): 498.42. (LCMS purity 99.62%, Rt=6.22 min (1)).

Synthesis of 117; 3-(2-(4-(4-(tert-butyl)phenylsulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)ethyl)pyridine 1-oxide

To a mixture of compound 116 (0.1 g, 0.21 mmol) and dichloromethane (4 mL) was added metachloroperoxybenzoic acid (0.069 g, 0.22 mmol). The reaction was stirred at RT for 5 h whereupon the solvent was concentrated under reduced pressure and the residue diluted with water. The aqueous layer was extracted with dichloromethane. The combined organic layers were washed sequentially with sodium bicarbonate and brine, then dried over Na₂SO₄, filtered and concentrated under vacuum to leave the crude compound which was purified by column chromatography using 3% methanol in dichloromethane to afford 3-(2-(4-(4-(tert-butyl)phenylsulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)ethyl)pyridine 1-oxide as an off white solid (117; 0.016 g, 15.5% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.56 (bs, 1H), 8.17 (s, 1H), 8.07-8.05 (d, J=6.4 Hz, 1H), 7.89-7.87 (m, 2H), 7.73-7.71 (d, J=8.4 Hz, 1H), 7.64-7.58 (m, 3H), 7.30-7.26 (m, 1H), 7.17-7.15 (m, 1H), 3.78-3.74 (t, J=6.8 Hz, 2H), 2.85-2.82 (t, J=6.8 Hz, 2H), 1.27 (s, 9H). MS (M+1): 514.45. (LCMS purity 99.01%).

Example 17

Synthesis of Compound 118 [4-(tert-butyl)-N-(7-chloro-2-(6-methoxypyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide] and Compound 119

Synthesis of LIII:

To a stirred solution of compound XXV (1.5 g, 6.63 mmol) in acetic acid (10 mL) was added 6-methoxypyridin-3-amine (LII, 0.98 g, 7.96 mmol). The reaction mixture was heated at 100° C. for 12 h. After cooling to room temperature, the acetic acid removed under reduced pressure to obtain the crude product which was washed with ethanol to afford 4-chloro-2-(6-methoxypyridin-3-yl)-7-nitroisoindoline-1,3-dione (LIII, 1.5 g, 68.18%). MS (M+1): 334.06.

Synthesis of LIV:

To a solution of compound LIII (1.5 g, 4.5 mmol) in acetic acid (9 mL) was added iron powder (1.3 g) in small portions. The reaction mixture was stirred for 12 h at room temperature and then filtered through a celite bed and concentrated under reduced pressure. The crude material was neutralized using aqueous sodium bicarbonate solution. The aqueous layer was extracted with ethyl acetate which was dried (anhydrous Na₂SO₄), filtered and evaporated under reduced pressure to afford 4-amino-7-chloro-2-(6-methoxypyridin-3-yl)isoindoline-1,3-dione as a greenish coloured solid (LIV; 0.8 g, 58.8% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.21 (s, 1H), 7.88-7.87 (d, J=6.4 Hz, 1H), 7.48-7.46 (d, J=8.8 Hz, 1H), 7.07-7.04 (d, J=9.2 Hz, 1H), 6.98-6.96 (d, J=8.8 Hz, 1H), 6.64 (bs, 2H), 3.89 (s, 3H). MS (M+1): 304.14. (LCMS purity 95.16%).

Synthesis of 118; 4-(tert-butyl)-N-(7-chloro-2-(6-methoxypyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide

A mixture of compound LIV (0.55 g, 1.8 mmol) and pyridine (4 mL) was cooled to 0° C. and 4-tert-butylbenzenesulfonyl chloride (V, 1.47 g, 6.35 mmol) was added together with a catalytic quantity of DMAP. The reaction mixture was heated for 12 h at 100° C. then concentrated and diluted with water. The aqueous layer was extracted with ethyl acetate. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound. To this material was added 1M TBAF in THF solution (2 mL) and the stirring continued for 1 h at room temperature. The reaction mixture was concentrated and diluted with ethyl acetate. The organic layer was washed with water, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound which was purified by column chromatography using 40% ethyl acetate in hexane to afford 4-(tert-butyl)-N-(7-chloro-2-(6-methoxypyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide as an off white solid (118; 0.5 g, 55.2% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.97 (bs, 1H), 8.19-8.18 (s, 1H), 7.94-7.92 (d, J=8.8 Hz, 2H), 7.82-7.80 (d, J=8.8 Hz, 1H), 7.75-7.72 (dd, J=2.8, 2.4 Hz, 1H), 7.68-7.63 (m, 3H), 7.0-6.98 (d, J=8.8 Hz, 1H), 3.90 (s, 3H), 1.27 (s, 9H). MS (M+1): 500.44. (LCMS purity 99.12%, Rt=6.50 min(1)).
The following compound was also prepared using a similar method and appropriate amine in the first step:


		LCMS	Purity
CPD	Structure	(M + 1)	(LCMS)	1HNMR

119		500.42	99.04%, Rt = 5.91 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.09 (bs, 1H), 8.59-8.57 (d, J = 6 Hz, 1H), 8.39 (s, 1H), 7.93-7.91 (m, 2H), 7.85-7.82 (d, J = 8.8 Hz, 1H), 7.67-7.62 (m, 3H). 7.31-7.30 (d, J = 5.6 Hz, 1H), 3.84 (s, 3H), 1.28 (s, 9H).

Example 18

Synthesis of Compound 120 [4-(tert-butyl)-N-(7-chloro-2-(3-cyanophenyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamidesulfonamide] and Compounds 121-125

Synthesis of LV:

To a stirred solution of compound XXV (3.0 g, 13 mmol) in acetic acid (65 mL) was added 3-aminobenzonitrile (XIX, 4.6 g, 38 mmol) and the reaction mixture was heated at 100° C. for 18 h. The reaction mixture was cooled to room temperature and the acetic acid removed under reduced pressure to obtain the crude product 3-(4-chloro-7-nitro-1,3-dioxoisoindolin-2-yl)benzonitrile as a brown solid (LV; 3 g,). MS (M+1): 328.02. The crude material was carried forward to next step without purification.

Synthesis of LVI:

To a solution of compound LV (2.5 g, 7.64 mmol) in acetic acid (38 mL) was added iron powder (0.85 g, 15 mmol) and the reaction mixture was stirred for 12 h at room temperature. The acetic acid was removed under reduced pressure to obtain a crude product. The reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and evaporated under reduced pressure before being triturated with tert-butylmethylether to obtain crude product 3-(4-amino-7-chloro-1, 3-dioxoisoindolin-2-yl)benzonitrile as a greenish solid (LVI; 2 g). MS (M+1) 298.07

Synthesis of 120; 4-(tert-butyl)-N-(7-chloro-2-(3-cyanophenyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide

A mixture of compound LVI (0.5 g, 1.68 mmol) and pyridine (5 mL) was cooled to 0° C. and 4-tert-butylbenzenesulfonyl chloride (V 1.16 g, 5.04 mmol) was added together with a catalytic quantity of DMAP (0.05 g, 0.016 mmol). The reaction mixture was stirred for 24 h at 80° C. The reaction mixture was concentrated and to the resultant residue, water was added and extracted with ethyl acetate. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound. This was purified by combiflash chromatography using 18% ethyl acetate in hexane as mobile phase to afford 4-(tert-butyl)-N-(7-chloro-2-(3-cyanophenyl)-1,3-dioxoisoindolin-4-1)benzenesulfonamide as a white solid (120, 0.04 g). ¹H NMR (400 MHz, DMSO-d6): δ 9.99 (bs, 1H), 7.93-7.91 (m, 3H), 7.88 (m, 1H) 7.85-7.82 (m, 1H) 7.78-7.76 (m, 2H), 7.70-7.68 (m, 1H), 7.65-7.63 (m, 2H), 1.27 (s, 9H). MS (M−1): 492.45 (LCMS Purity 98.22%, Rt=6.59 min (1)).
The following compounds were prepared in a similar manner using the appropriate sulfonyl chloride in the final step:


		LCMS	Purity
Cpd	Structure	(M − 1)	(LCMS)	¹H NMR

121		504.40	98.92%, Rt = 5.79 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.63 (bs, 1H), 8.15-8.13 (d, J = 8.4 Hz, 2H), 8.02-8.00 (d, J = 8.4 Hz, 2H), 7.95-7.94 (d, J = 7.2 Hz, 1H), 7.85-7.83 (d, J = 9.2 Hz, 2H) 7.78- 7.72 (m, 2H), 7.64-7.62 (d, J = 8.8 Hz, 1H).

122		520.36	99.01%, Rt = 5.78 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.38 (bs, 1H), 8.10-8.08 (m, 2H), 7.95-7.94 (m, 1H), 7.85-7.83 (m, 2H), 7.79-7.75 (m, 2H), 7.66-7.60 (m, 3H).

123		538.31	98.74%, Rt = 5.77 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.86 (bs, 1H), 8.43 (s, 1H) 8.19- 8.17 (d, J = 8.0 Hz, 1H), 8.03-7.91 (m, 2H), 7.84-7.82 (m, 2H), 7.77- 7.72 (m, 2H), 7.64-7.61 (d, J = 8.8 Hz, 1H).

124		501.99	99.07%, Rt = 5.68 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.16 (bs, 1H), 8.07-8.04 (d, J = 8.8 Hz, 2H), 7.96-7.94 (dd, J = 2.8, 2.8 Hz, 1H), 7.87 (s, 1H), 7.84-7.82 (d, J = 8.8 Hz, 1H), 7.77-7.76 (d, J = 9.2 Hz, 2H), 7.67-7.64 (d, J = 9.2 Hz, 1H), 7.59-7.22 (m, 3H).

125		478.30	97.07%, Rt = 6.30 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.0 (bs, 1H), 7.96-7.91 (m, 3H), 7.88 (d, J = 1.2 Hz, 1H), 7.84-7.82 (d, J = 8.8 Hz, 1H), 7.78-7.77 (m, 2H), 7.69-7.67 (d, J = 8.8 Hz, 1H), 7.51-7.49 (d, J = 8.4 Hz, 2H), 2.99- 2.95 (m, 1H), 1.19-1.18 (d, J = 6.8 Hz, 6H).

Example 19

Synthesis of Compound 126 [4-(tert-butyl)-N-(7-chloro-2-(3-cyanobenzyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide]; Compound 127 [4-(tert-butyl)-N-(7-cyano-2-(3-cyanobenzyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide] and Compounds 128-135

Synthesis of LVII:

To a stirred solution of compound XXV (3.5 g, 15 mmol) in acetic acid (70 mL) was added 3-(aminomethyl)benzonitrile (XXVII, 6.07 g, 46 mmol) and the reaction mixture heated at 100° C. for 18 h. The reaction mixture was cooled to room temperature and the acetic acid removed under reduced pressure to obtain the crude product 3-((4-chloro-7-nitro-1,3-dioxoisoindolin-2-yl)methyl)benzonitrile as a brown solid (LVII; 4.8 g,). ¹H NMR (400 MHz, DMSO-d6): δ 8.28-8.26 (d, J=8.4 Hz, 1H), 8.12-8.10 (d, J=8.4 Hz, 1H), 7.85 (s, 1H), 7.76-7.72 (m, 2H), 7.58-7.54 (t, J=7.6 Hz, 1H), 4.83 (s, 2H). MS (M+1): 342.02.

Synthesis of LVIII:

To a solution of compound LVII (2.5 g, 7 mmol) in acetic acid (38 mL) was added iron powder (0.85 g, 15 mmol) and the reaction mixture was stirred at room temperature for 12 h. The acetic acid was removed under reduced pressure to obtain the crude product. The reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and concentrated under reduced pressure. This material was triturated with tert-butylmethylether to obtain the crude product 3-((4-amino-7-chloro-1,3-dioxoisoindolin-2-yl)methyl)benzonitrile as a greenish solid (LVIII; 2 g). MS (M+1) 312.

Synthesis of 126; 4-(tert-butyl)-N-(7-chloro-2-(3-cyanobenzyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide

To a stirred mixture of compound LVIII (0.4 g, 1.28 mmol) in chloroform (10 mL) was added pyridine (3 mL) at 0° C. followed by addition of 4-tert-butylbenzenesulfonyl chloride (V, 0.89 g, 3.84 mmol) and a catalytic quantity of DMAP. The reaction mixture was stirred at 80° C. for 24 h. The reaction mixture was cooled and concentrated at reduced pressure to afford a mixture of the mono and di-substituted sulfonamide product. This was dissolved in THF (15 mL) in presence of 1M TBAF in THF solution (1 mL) and stirred at 90° C. for 5 h. The reaction mixture was concentrated at reduced pressure, diluted with water and the aqueous layer was extracted with ethyl acetate. The combined organic layers were washed with brine, dried over Na₂SO₄, filtered and concentrated to afford the crude compound, which was purified by column chromatography using 20% ethyl acetate in hexane to afford the title compound 4-(tert-butyl)-N-(7-chloro-2-(3-cyanobenzyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide (126; 0.04 g;). ¹H NMR (400 MHz, DMSO-d6): δ 9.94 (bs, 1H), 7.94-7.92 (d, J=8.4 Hz, 2H), 7.83 (s, 1H), 7.76-7.73 (m, 2H), 7.69-7.67 (m, 1H), 7.63-7.59 (m, 3H), 7.56-7.53 (m, 1H), 4.76 (s, 2H), 1.26 (s, 9H). MS (M−1): 506.40. (LCMS purity 97.23%, Rt=6.64 min (1)).

Synthesis of 127; 4-(tert-butyl)-N-(7-cyano-2-(3-cyanobenzyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide

To a stirred solution of compound 126 (0.1 g, 0.19 mmol) in dimethylacetamide (10 mL) was added Zn(CN)₂(0.046 g, 0.39 mmol) and the reaction vessel was purged with argon for 20 min. Then 1,1′-Bis(diphenylphosphino)ferrocene (0.021 g, 0.038 mmol), Pd₂dba₃(0.026 g, 0.028 mmol) and a catalytic quantity of Zn dust were added. The reaction mixture was stirred at 120° C. for 2 h in microwave reactor. The reaction mixture was cooled and concentrated at reduced pressure, diluted with water and the aqueous layer was extracted with ethyl acetate. The combined organic layers were washed with brine, dried over Na₂SO₄, filtered and concentrated to afford the crude compound, which was purified by prep HPLC to obtain 4-(tert-butyl)-N-(7-chloro-2-(3-cyanobenzyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide, (127; 0.02 g, 20.4% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.10-8.07 (d, J=8.0 Hz, 2H), 7.84 (s, 1H), 7.77-7.76 (d, J=8.4 Hz, 1H), 7.72-7.62 (m, 5H), 7.57-7.53 (t, J=8.0 Hz, 1H), 4.79 (s, 2H), 1.27 (s, 9H). MS (M−1): 497.41. (LCMS purity 99.89%, Rt=5.60 min (1)).
The following compounds were prepared in a similar manner using the appropriate sulfonyl chloride in the penultimate step:


		LCMS	Purity
CPD	Structure	(M − 1)	(LCMS)	¹H NMR

128		518.29	99.64%, Rt = 5.82 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.47 (bs, 1H), 8.14-8.12 (d, J = 8.0 Hz, 2H), 7.97-7.95 (d, J = 8.4 Hz, 2H), 7.80 (s 1H), 7.77-7.54 (m, 2H), 7.64-7.62 (d, J = 8.0 Hz, 1H), 7.56-7.52 (m, 2H), 4.73 (s 2H).

129		534.31	99.17%, Rt = 6.01 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.29 (bs, 1H), 8.07-8.05 (m, 2H), 7.80-7.75 (m, 3H), 7.65-7.63 (d, J = 7.2 Hz, 1H), 7.58-7.52 (m, 4H), 4.74 (s 2H).

130		518.04	97.74%, Rt = 5.74 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.52 (bs, 1H), 8.28 (s, 1H), 8.20- 8.18 (d, J = 8.0 Hz, 1H), 8.03-8.01 (d, J = 8.0 Hz, 1H), 7.83-7.75 (m, 4H), 7.63-7.53 (m, 3H), 4.72 (s 2H).

131		515.99	98.72%, Rt = 5.90 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.09 (bs, 1H), 8.04-8.02 (d, J = 8.8 Hz, 2H), 7.82 (s, 1H), 7.76- 7.73 (m, 2H), 7.66-7.64 (d, J = 7.6 Hz, 1H), 7.58-7.52 (m, 2H), 7.38- 7.20 (m, 3H), 4.75 (s, 2H).

132		552.16	99.55%, Rt = 5.89 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.70 (bs, 1H), 8.38 (s, 1H), 8.17- 8.15 (d, J = 8.4 Hz, 1H), 7.94-7.92 (d, J = 8.4 Hz, 1H), 7.80-7.74 (m, 3H), 7.64-7.62 (d, J = 7.6 Hz, 1H), 7.56-7.52 (m, 2H), 4.73 (s, 2H).

133		536.15	99.53%, Rt = 5.78 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.58 (bs, 1H), 8.36-8.35 (d, J = 5.2 Hz, 1H), 8.25 (s, 1H), 7.79- 7.68 (m, 4H), 7.63-7.61 (d, J = 7.6 Hz, 1H), 7.58-7.52 (m, 2H), 4.73 (s, 2H).

134		517.06	99.03%, Rt = 5.83 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.13 (bs, 1H), 8.06-8.04 (m, 2H) 7.83 (s, 1H), 7.76-7.74 (m, 4H). 7.68-7.66 (d, J = 6.8 Hz, 1H) 7.61- 7.53 (m, 2H), 4.76 (s, 2H), 1.68 (s, 6H).

135		517.13	98.84%, Rt = 5.51 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.15 (bs, 1H), 8.56 (s, 1H), 8.04- 8.02 (d, J = 8.4 Hz, 2H), 7.92-7.90 (m, 3H), 7.81 (s, 1H), 7.76-7.71 (t, J = 9.2 Hz, 2H), 7.63-7.57 (m, 2H), 7.52-7.48 (t, J = 7.6 Hz, 1H), 4.74 (s, 2H).

Example 20

Synthesis of Compound 136 [4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(3-(trifluoromethyl)phenyl)iso indolin-4-yl)benzenesulfonamide] and Compounds 137-146

Synthesis of LX:

To a stirred solution of compound XXV (5 g, 22.12 mmol) in acetic acid (100 mL) was added 3-(trifluoromethyl)aniline (LIX, 8.9 g, 55 mmol) and the reaction mixture heated at 100° C. for 12 h. The reaction mixture was cooled to room temperature, diluted and triturated with ethanol to afford 4-chloro-7-nitro-2-(3-(trifluoromethyl)phenyl)isoindoline-1,3-dione (LX, 7.5 g, 91.6%). ¹H NMR (400 MHz, DMSO-d6): δ 8.36 (m, 1H), 8.21 (m, 2H), 7.85 (m, 1H), 7.54 (m, 1H), 7.38 (m, 1H).

Synthesis of LXI:

To a solution of compound LX (5 g, 13.5 mmol) in acetic acid (100 mL) was added iron powder (5 g) in small portions. The reaction mixture was stirred for 12 h at room temperature. The reaction mixture was filtered through a celite bed and concentrated under reduced pressure. The crude material was neutralized using aqueous sodium bicarbonate solution. The aqueous layer was extracted with ethyl acetate which was dried (anhydrous Na₂SO₄), filtered and evaporated under reduced pressure to afford compound 4-amino-7-chloro-2-(3-(trifluoromethyl)phenyl)isoindoline-1,3-dione as a yellow solid (LXI; 4 g, 87% yield). ¹H NMR (400 MHz, DMSO-d6): δ 7.86 (s, 1H), 7.77 (m, 3H), 7.50-7.48 (d, J=9.2 Hz, 1H), 7.08-7.05 (d, J=8.8 Hz, 1H), 6.73 (bs, 2H).

Synthesis of 136; 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(3-(trifluoromethyl)phenyl) isoindolin-4-yl)benzenesulfonamide

A mixture of compound LXI (0.3 g, 0.88 mmol) and pyridine (5 mL) was cooled to 0° C. and 4-tert-butylbenzenesulfonyl chloride (V, 0.62 g, 2.64 mmol) was added followed by catalytic DMAP (0.053 g, 0.44 mmol). The reaction mixture was heated for 15 h at 100° C. The reaction mixture was concentrated and diluted with water and then extracted with ethyl acetate. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound. This was purified by column chromatography using 70% ethyl acetate in hexane to afford 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(3-(trifluoromethyl)phenyl)isoindolin-4-yl)benzenesulfonamide as a off white solid (136; 0.25 g, 53% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.98 (bs, 1H), 7.94-7.92 (d, J=8.4 Hz, 2H), 7.84-7.74 (m, 5H), 7.70-7.68 (d, J=8.8 Hz, 1H), 7.65-7.63 (d, J=8.4 Hz, 2H), 1.27 (s, 9H). MS (M−1): 535.28. (LCMS purity 99.12%, Rt=6.84 min (1)).
The following compounds were also prepared using a similar method and the appropriate amine in the first step and/or sulfonyl chloride in the final step:


		LCMS	Purity
CPD	Structure	(M − 1)	(LCMS)	¹H NMR

137		565.23	97.22%, Rt = 6.06 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.66 (bs, 1H), 8.42-8.41 (d, J = 8.4 Hz, 1H), 8.32-8.29 (m, 1H), 7.84-7.79 (m, 5H), 7.72 (m, 1H), 7.66-7.63 (d, J = 8.8 Hz, 1H).

138		497.24	99.92% Rt = 6.72 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 9.92 (bs, 1H), 7.93-7.91 (d, J = 8.0 Hz, 2H), 7.82-7.79 (d, J = 8.8 Hz, 1H), 7.68-7.63 (m, 3H), 7.44-7.41 (t, J = 8.0 Hz, 1H), 7.04-6.94 (m, 3H), 3.77 (s, 3H), 1.27 (s, 9H).

139		471.38 (M + 1)	97.95% Rt = 5.84 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.13 (bs, 1H), 9.26 (s, 1H), 8.91 (s, 2H), 7.94-7.92 (d, J = 8.8 Hz, 2H), 7.87-7.85 (d, J = 8.4 Hz, 1H), 7.70- 7.68 (d, J = 8.8 Hz, 1H), 7.65-7.63 (d, J = 8.4 Hz, 2H), 1.27 (s, 9H).

140		481.28	98.45%, Rt = 5.13 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.67 (bs, 1H), 9.26 (s, 1H), 8.87 (s, 2H), 8.17-8.15 (d, J = 8.4 Hz, 2H), 8.02-8.0 (d, J = 8.4 Hz, 2H), 7.87- 7.85 (d, J = 8.8 Hz, 1H), 7.64-7.62 (d, J = 8.8 Hz, 1H).

141		457.37	99.37%, Rt = 5.63 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.10 (bs, 1H), 9.26 (s, 1H), 8.61 (s, 2H), 7.94-7.92 (d, J = 8.0 Hz, 2H), 7.86-7.84 (d, J = 8.8 Hz, 1H), 7.70- 7.68 (d, J = 9.2 Hz, 1H), 7.51-7.49 (d, J = 8.4 Hz, 2H), 3.0-2.9 (m, 1H), 1.19-1.18 (d, J = 6.4 Hz, 6H).

142		473.44 (M + 1)	98.88% Rt = 6.27 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 9.93 (bs, 1H), 8.07 (s, 1H), 7.92-7.90 (d, J = 8.4 Hz, 2H), 7.78-7.75 (d, J = 8.8 Hz, 1H), 7.72 (s, 1H), 7.64-7.61 (m, 3H), 3.89 (s, 3H), 1.26 (s, 9H).

143		485.32 (M + 1)	99.61%, Rt = 5.35 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.45 (bs, 1H), 8.16-8.14 (d, J = 8.0 Hz, 2H), 8.04 (s, 1H), 8.01-7.99 (d, J = 8.0 Hz, 2H), 7.79-7.76 (d, J = 8.8 Hz, 1H), 7.69 (s, 1H), 7.58-7.56 (d, J = 8.8 Hz, 1H), 3.82 (s, 3H).

144		473.36 (M + 1)	99.34% Rt = 5.90 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.04 (bs, 1H), 7.95-7.93 (d, J = 8.4 Hz, 2H), 7.84-7.82 (d, J = 8.8 Hz, 1H), 7.68-7.63 (m, 3H), 7.56 (d, J = 1.6 Hz, 1H), 6.33 (d, J = 1.6 Hz, 1H), 3.68 (s, 3H), 1.27 (s, 9H).

145		473.43 (M + 1)	97.95% Rt = 6.22 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 9.93 (bs, 1H), 7.91-7.88 (d, J = 8.4 Hz, 2H), 7.81-7.79 (d, J = 8.4 Hz, 2H), 7.62-7.61 (m, 3H), 6.28 (d, J = 1.6 Hz, 1H), 3.87 (s, 3H), 1.27 (s, 9H).

146		476.39 (M + 1)	99.19% Rt = 5.92 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.10 (bs, 1H), 7.93 (d, J = 8.4 Hz, 2H), 7.83-7.80 (m, 3H), 7.68 (d, J = 8.8 Hz, 1H), 7.64-7.62 (d, J = 8.4 Hz, 2H), 1.27 (s, 9H).

Example 21

Synthesis of Compound 147 [4-(tert-butyl)-N-(7-chloro-2-(3-methoxybenzyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide]

Synthesis of LXIII:

To a stirred solution of compound XXV (1 g, 4.39 mmol) in acetic acid (10 mL) was added (3-methoxyphenyl)methanamine (LXII, 1.2 g, 8.78 mmol). The reaction mixture was heated at 100° C. for 12 h. The reaction mixture was cooled to room temperature and the acetic acid removed under reduced pressure to obtain the crude product. This was washed with ethanol to afford 4-chloro-2-(3-methoxybenzyl)-7-nitroisoindoline-1,3-dione (LXIII, 1 g, 65.7%). ¹H NMR (400 MHz, DMSO-d6): δ 8.27-8.25 (d, J=8.8 Hz, 1H), 8.10-8.08 (d, J=8.4 Hz, 1H), 7.21-7.23 (t, J=8.0 Hz, 1H), 6.91 (m, 2H), 6.86-6.84 (d, J=8.0 Hz, 1H), 4.7 (s, 2H), 3.7 (s, 3H).

Synthesis of LXIV:

To a solution of compound LXIII (0.6 g, 1.72 mmol) in ethanol (80 mL) was added stannous chloride (0.97 g, 4.3 mmol). The reaction mixture was heated at 90° C. for 12 h. The reaction mixture was filtered through a celite bed and concentrated under reduced pressure. The crude material was diluted with water which was extracted with ethyl acetate. The organic solvent was evaporated under reduced pressure to afford 4-amino-7-chloro-2-(3-methoxybenzyl)isoindoline-1,3-dione as a yellow solid (LXIV; 0.4 g, 73% yield). ¹H NMR (400 MHz, DMSO-d6): δ 7.42-7.40 (d, J=8.8 Hz, 1H), 7.26-7.24 (t, J=8.4 Hz, 1H), 7.01-6.99 (d, J=9.2 Hz, 1H), 6.84-6.81 (m, 3H), 6.64 (bs, 2H), 4.65 (s, 2H), 3.72 (s, 3H). MS (M−1): 315.25. (LCMS purity 95.34%).

Synthesis of 147; 4-(tert-butyl)-N-(7-chloro-2-(3-methoxybenzyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide

A mixture of compound LXIV (0.2 g, 0.63 mmol) in THF (4 mL) was cooled to 0° C. and then sodium hydride (0.03 g, 1.26 mmol) was added followed by addition of 4-tert-butylbenzenesulfonyl chloride (16, 0.22 g, 0.91 mmol). The reaction mixture was stirred at room temperature for 4 h. The reaction mixture was diluted with water and the aqueous layer was extracted with ethyl acetate. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound. To this material was added 1M TBAF in THF solution (1 mL) and the stirring continued for 1 h at room temperature. The reaction mixture was concentrated and diluted with ethyl acetate. The organic layer was washed with water, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound which was purified by column chromatography using 15% ethyl acetate in hexane. The title compound 4-(tert-butyl)-N-(7-chloro-2-(3-methoxybenzyl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide was obtained as a yellow solid (147; 0.1 g, 30.8% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.95 (bs, 1H), 7.91-7.89 (d, J=8.0 Hz, 2H), 7.75-7.73 (d, J=9.2 Hz, 1H), 7.62-7.58 (m, 3H), 7.23-7.21 (t, J=7.6 Hz, 1H), 6.86-6.84 (m, 3H), 4.66 (s, 2H), 3.72 (s, 3H), 1.26 (s, 9H). MS (M+1): 511.38. (LCMS purity 98.66%, Rt=6.97 min (1)).

Example 22

Synthesis of Compound 148 [4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(1H-pyrazol-4-yl)isoindolin-4-yl)benzenesulfonamide] and Compounds 149-152

Synthesis of LXVI:

To a stirred solution of compound XXV (2.5 g, 10 mmol) in acetic acid (50 mL) was added 1H-pyrazol-4-amine (LXV; 2.2 g, 20 mmol) and the reaction mixture heated at 100° C. for 18 h. The reaction mixture was cooled to room temperature and the acetic acid removed under reduced pressure to obtain crude product. The crude product was washed with ethanol to afford 4-chloro-7-nitro-2-(1H-pyrazol-4-yl)isoindoline-1,3-dione as a yellow solid (LXVI; 2.5 g, 78% yield). ¹H NMR (400 MHz, DMSO-d6): v 13.18 (s, 1H), 8.29-8.27 (d, J=8 Hz, 1H), 8.13-8.11 (d, J=8 Hz, 2H), 7.84 (bs, 1H). MS (M+1): 292.93.

Synthesis of LXVII:

To a solution of compound LXVI (2.5 g, crude) in acetic acid (40 mL) was added iron powder (3 g) in small portions. The reaction mixture was stirred for 12 h at room temperature and filtered through a celite bed before being concentrated under reduced pressure. The crude mass was neutralized by aqueous sodium bicarbonate solution. This was extracted with ethyl acetate, which was dried (anhydrous Na₂SO₄), filtered and evaporated under reduced pressure to obtain the crude compound. This was purified further by trituration using ethanol to afford compound 4-amino-7-chloro-2-(1H-pyrazol-4-yl)isoindoline-1,3-dione as a yellow solid (LXVII; 2 g, 86% yield). ¹H NMR (400 MHz, DMSO-d6): δ 13.13 (s, 1H), 7.94 (s, 2H), 7.45-7.42 (d, J=12 Hz, 1H), 7.03-7.01 (d, J=8.8 Hz, 1H), 6.71 (s, 2H). MS (M+1): 263.1

Synthesis LXVIII:

To stirred solution of compound LXVII (0.8 g, 3 mmol) in pyridine (50 mL) was added 4-(tert-butyl)benzene-1-sulfonyl chloride (V, 2.83 g, 12.2 mmol) and heated the reaction mixture for 12 h at 100° C. The reaction mixture was concentrated and then diluted with water. The aqueous layer was extracted with chloroform, which was washed with brine solution and dried over anhydrous Na₂SO₄, filtered and evaporated under reduced pressure to obtain crude compound which was partially purified by column chromatography using 50% ethyl acetate in hexane to obtain a mixture of products (4-(tert-butyl)-N-(2-(1-((4-(tert-butyl)phenyl)sulfonyl)-1H-pyrazol-4-yl)-7-chloro-1,3-dioxoisoindolin-4-yl)benzenesulfonamide (LXVII) and material where the sulfonamide bond para to the chloro group had not formed. This mixture was used without further purification.

Synthesis of 148; 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(1H-pyrazol-4-yl)isoindolin-4-yl)benzenesulfonamide

To the crude compound LXVII (350 mg), was added 1M TBAF in THF solution (30 mL) and the reaction mixture was stirred for 1 h at room temperature. This was diluted with water and extracted with chloroform. The resulting organic layer was washed with brine solution and dried over anhydrous Na₂SO₄, filtered and then evaporated under reduced pressure to afford crude compound which was purified by column chromatography using 60% ethyl acetate in hexane to afford 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(1H-pyrazol-4-yl)isoindolin-4-yl)benzenesulfonamide as a off white solid. (148, 0.015 g). ¹H NMR (400 MHz, DMSO-d6): δ 13.14 (bs, 1H), 9.96 (s, 1H), 8.07 (s, 1H), 7.94-7.92 (d, J=6.8 Hz, 2H), 7.78-7.76 (m, 2H), 7.64-762 (m, 2H), 1.26 (s, 9H). MS (M−1): 457.04. (LCMS purity 97.12%, Rt=6.12 min (1)).
The following compounds were also prepared using a similar method and the appropriate sulfonyl chloride in the final step:


		LCMS	Purity
CPD	Structure	(M + 1)	(LCMS)	¹H NMR

149		469.33 (M − 1)	98.58%, Rt = 5.06 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 13.14 (bs, 1H), 10.50 (bs, 1H), 8.15- 8.13 (d, J = 8.0 Hz, 2H), 8.06-7.71 (m, 5H), 7.58-7.56 (d, J = 8.8 Hz, 1H).

150		486.97	99.17%, Rt = 6.32 min (2)	¹H NMR (400 MHz, DMSO-d6): δ 13.14 (bs, 1H), 10.31 (bs, 1H), 8.10- 8.07 (d, J = 8.8 Hz, 2H), 7.92-7.77 (m, 3H), 7.61-7.58 (m, 3H).

151		443.33 (M − 1)	98.98%, Rt = 5.78 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 13.15 (bs, 1H), 9.94 (bs, 1H), 8.07 (bs, 1H), 7.92-7.89 (d, J = 8.4 Hz, 2H), 7.80 (bs, 1H), 7.77-7.75 (d, J = 8.8 Hz, 1H), 7.63-7.61 (d, J = 8.8 Hz, 1H), 7.49-7.47 (d, J = 8.4 Hz, 2H), 2.99-2.92 (m, 1H), 1.18-1.16 (d, J = 7.2 Hz, 6H).

152		467.25 (M − 1)	98.83%, Rt = 5.09 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 13.14 (bs, 1H), 10.12 (bs, 1H), 8.05- 8.03 (d, J = 8.4 Hz, 2H), 7.84 (m, 1H), 7.77-7.75 (m, 2H), 7.60-7.58 (d, J = 9.2 Hz, 1H), 7.40-7.36 (m, 2H), 7.21 (m, 1H).

Example 23

Synthesis of Compound 153 [4-(tert-butyl)-N-(7-chloro-2-(1-methyl-1H-imidazol-4-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide] and Compound 154

Synthesis of LXX:

To stirred solution of 4-nitro-1H-imidazole (LXIX, 5 g: 44 mmol) in acetonitrile (20 mL) was added K₂CO₃(18.2 g, 137 mmol) at 0° C., followed by addition of methyl iodide (8.9 mL, 57 mmol). The reaction mixture was heated at 95° C. for 3 h. The reaction mixture was concentrated, filtered through a celite bed and diluted with water. The aqueous layer was extracted with chloroform and the separated organic layer was washed with brine solution and dried over anhydrous Na₂SO₄before being filtered and evaporated under reduced pressure to afford 1-methyl-4-nitro-1H-imidazole (LXX, 4 g, 71%). ¹H NMR (400 MHz, DMSO-d6) δ 8.35 (s, 1H), 7.8 (s, 1H), 3.74 (s, 3H). MS (M+1): 128.

Synthesis of LXXI:

To a stirred solution of compound LXX (4 g, 31 mmol) in ethanol (20 mL) was added 10% Pd—C (catalytic) under nitrogen atmosphere. The reaction mixture was evacuated using high vacuum and stirred in presence of hydrogen gas at balloon pressure at room temperature for 6 h. The reaction mixture was filtered through a celite bed. The organic solvent was evaporated under reduced pressure to afford 1-methyl-1H-imidazol-4-amine (LXXI, 2.5 g) MS (M+1) 98. The crude material was carried forward to next step without purification.

Synthesis of LXXII:

To a stirred solution of compound XXV (3 g, 13.2 mmol) in acetic acid (60 mL) was added 1-methyl-1H-imidazol-4-amine (LXXI, 3.2 g, 33 mmol). The reaction mixture was heated at 100° C. for 12 h. The reaction mixture was cooled to room temperature and the acetic acid removed under reduced pressure to obtain crude product. The crude product was washed with ethanol to afford 4-chloro-2-(1-methyl-1H-imidazol-4-yl)-7-nitroisoindoline-1,3-dione (LXXII, 3 g). MS (M+1): 306.84

Synthesis of LXXIII:

To a solution of compound LXXII (3 g, 98 mmol) in acetic acid (50 mL) was added iron powder (3 g) in small portions. The reaction mixture was stirred for 12 h at room temperature and then filtered through a celite bed and concentrated under reduced pressure. The crude material was neutralized by addition of aqueous sodium bicarbonate solution. The resulting aqueous layer was extracted with ethyl acetate. The layers were separated and the organic layer was dried (anhydrous Na₂SO₄), filtered and evaporated under reduced pressure to obtain the crude compound. This was purified using a neutral alumina column and 5% methanol in dichloromethane to afford 4-amino-7-chloro-2-(1-methyl-1H-imidazol-4-yl)isoindoline-1,3-dione as a yellow solid (LXXIII; 0.6 g, 22% yield). MS (M+1): 277.04.

Synthesis of 153; 4-(tert-butyl)-N-(7-chloro-2-(1-methyl-1H-imidazol-4-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide

A mixture of compound LXXIII (0.6 g, 2.16 mmol) and pyridine (20 mL) was cooled to 0° C. and 4-tert-butylbenzenesulfonyl chloride (V, 1.2 g, 5.5 mmol) was added. The reaction mixture was stirred for 12 h at 100° C. and then concentrated and diluted with water whereupon it was extracted with ethyl acetate. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and the organic solvent evaporated under reduced pressure to obtain the crude compound. To this material was added 1M TBAF in THF solution (25 mL) and the stirring continued for 1 h at room temperature. The reaction mixture was concentrated and diluted with ethyl acetate and the resulting solution was washed with water, dried over anhydrous Na₂SO₄, filtered and the organic solvent evaporated under reduced pressure to obtain the crude compound which was purified by column chromatography using 60% ethyl acetate in hexane to afford 4-(tert-butyl)-N-(7-chloro-2-(1-methyl-1H-imidazol-4-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide as an off white solid (153; 0.38 g, 37% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.93 (bs, 1H), 7.91-7.89 (d, J=8.0 Hz, 2H), 7.80-7.78 (d, J=8.4 Hz, 1H), 7.65-7.61 (m, 4H), 7.24 (s, 1H), 3.71 (s, 3H), 1.27 (s, 9H). MS (M+1): 473.36. (LCMS purity 99.27%, Rt=5.78 min(2)).
The following compound was also prepared using a similar method and the appropriate sulfonyl chloride in the final step:


		LCMS	Purity
CPD	Structure	(M + 1)	(LCMS)	1HNMR

154		485.31	97.54%, Rt = 4.97 min (1)	¹H NMR (400 MHz, DMSO-d6): δ 10.5 (bs, 1H), 8.15-8.13 (d, J = 8.0 Hz, 2H), 8.0-7.98 (d, J = 8.4 Hz, 2H), 7.81-7.79 (d, J = 8.8 Hz, 1H), 7.66 (s, 1H), 7.60- 7.58 (d, J = 8.8 Hz, 1H), 7.21 (s, 1H), 3.71 (s, 3H).

Example 24

Synthesis of Compound 155 [methyl 3-(4-((4-(tert-butyl)phenyl)sulfonamido)-7-chloro-1,3-dioxo isoindolin-2-yl)thiophene-2-carboxylate]

Synthesis of LXXV:

To a stirred solution of compound XXV (0.5 g, 2.2 mmol) in acetic acid (4.5 mL) was added methyl 3-aminothiophene-2-carboxylate (LXXIV, 0.69 g, 4.4 mmol). The reaction mixture was heated at 120° C. for 12 h and after cooling to room temperature, the acetic acid was removed under reduced pressure to obtain the crude product. This was triturated with ethanol to afford methyl 3-(4-chloro-7-nitro-1, 3-dioxoisoindolin-2-yl)thiophene-2-carboxylate (LXXV, 0.5 g, crude), which was carried forward to next step without purification.

Synthesis of LXXVI:

To a solution of compound LXXV (0.5 g, crude) in acetic acid (10 mL) was added iron powder (0.5 g) in portions. The reaction mixture was stirred for 12 h at room temperature and then filtered through a celite bed and concentrated under reduced pressure. The crude mass was neutralized using aqueous sodium bicarbonate solution and the aqueous layer was extracted with ethyl acetate. The organic solvent was separated, dried (anhydrous Na₂SO₄) filtered and evaporated under reduced pressure to obtain the crude compound methyl 3-(4-amino-7-chloro-1,3-dioxoisoindolin-2-yl)thiophene-2-carboxylate as a greenish solid (LXXVI; 0.35 g, 76% yield). ¹H NMR (400 MHz, DMSO-d6): □ 8.04 (d, J=3.6 Hz, 1H), 7.50-7.48 (d, J=4 Hz, 1H), 7.29-7.28 (d, J=3.6 Hz, 1H), 7.09-7.07 (d, J=8.4 Hz, 1H), 6.74 (bs, 2H), 3.72 (s, 3H).

Synthesis of 155; methyl 3-(4-((4-(tert-butyl)phenyl)sulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)thiophene-2-carboxylate

A mixture of compound LXXVI (0.35 g, 1.04 mmol) and pyridine (2 mL) was cooled to 0° C. and 4-tert-butylbenzenesulfonyl chloride (V, 0.48 g, 2.08 mmol) was added. The reaction mixture was stirred for 12 h at 100° C. and on cooling was concentrated and diluted with water. The aqueous layer was extracted with ethyl acetate which was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and evaporated under reduced pressure to obtain the crude compound. To the crude material was added TBAF in THF solution (2 mL) and the stirring continued for 1 h at room temperature. The reaction mixture was concentrated and diluted with ethyl acetate, which was washed with water, dried over anhydrous Na₂SO₄, filtered and evaporated under reduced pressure to leave the crude compound which was purified by column chromatography using 40% ethyl acetate in hexane to afford the title compound methyl 3-(4-((4-(tert-butyl)phenyl)sulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)thiophene-2-carboxylate as a off white solid (155; 0.13 g, 24.3% yield). ¹H NMR (400 MHz, DMSO-d6): δ 10.08 (bs, 1H), 8.08-8.07 (d, J=5.2 Hz, 1H), 7.92-7.90 (d, J=8.8 Hz, 2H), 7.86-7.84 (d, J=8.0 Hz, 1H), 7.70-7.68 (d, J=9.2 Hz, 1H), 7.64-7.62 (d, J=8.4 Hz, 2H), 7.25-7.24 (d, J=4.8 Hz, 1H), 3.70 (s, 3H), 1.27 (s, 9H). MS (M−1): 531.25. (LCMS purity 99.67%, Rt=6.61 min (1)).

Example 25

Synthesis of Compound 156 [4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(tetrahydro-2H-pyran-4-yl)iso indolin-4-yl)benzenesulfonamide]

Synthesis of LXXVIII:

To a stirred solution of compound XXV (1 g, 4.40 mmol) in acetic acid (30 mL) was added tetrahydro-2H-pyran-4-amine (LXXVII, 2 g, 19.7 mmol). The reaction mixture was heated at 120° C. for 12 h whereupon it was cooled to room temperature and the acetic acid removed under reduced pressure to obtain crude product. This was triturated with ethanol to afford 4-chloro-7-nitro-2-(tetrahydro-2H-pyran-4-yl)isoindoline-1,3-dione (LXXVIII, 0.7 g). This unpurified material was carried forward to next step without purification.

Synthesis of LXXIX:

To a solution of compound LXXVIII (0.7 g, 2.25 mmol) in ethanol (15 mL) was added stannous chloride (1.27 g, 5.6 mmol). The reaction mixture was heated at 90° C. for 12 h. The reaction mixture was filtered through a celite bed and concentrated under reduced pressure. The crude mass was diluted with water and the aqueous layer was extracted with ethyl acetate. The organic solvent was dried (anhydrous Na₂SO₄), filtered and evaporated under reduced pressure to afford 4-amino-7-chloro-2-(tetrahydro-2H-pyran-4-yl)isoindoline-1,3-dione an off-white solid (LXXIX; 0.2 g, 31.7% yield). ¹H NMR (400 MHz, DMSO-d6): δ 7.40-7.38 (d, J=8.8 Hz, 1H), 6.99-6.96 (d, J=8.8 Hz, 1H), 6.60 (bs, 2H), 4.15 (m, 1H), 3.95-3.92 (m, 2H), 3.40-3.37 (m, 2H), 2.34-2.25 (m, 2H), 1.61-1.57 (m, 2H). MS (M−1): 279.20.

Synthesis of 156; 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(tetrahydro-2H-pyran-4-yl)iso indolin-4-yl)benzenesulfonamide

Compound LXXIX (0.16 g, 0.57 mmol) was dissolved in a mixture of chloroform (10 mL) and pyridine (15 mL) was cooled to 0° C. and a catalytic quantity of DMAP (0.07 g, 0.057 mmol) was added. To the reaction mixture was further added 4-tert-butylbenzenesulfonyl chloride (V, 0.33 g, 1.43 mmol). The reaction mixture was heated at 100° C. for 12 h before being cooled and then diluted with water. The reaction mixture was extracted with ethyl acetate and the organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and the organic solvent evaporated under reduced pressure to obtain the crude compound. This material was purified by column chromatography using 20% ethyl acetate in hexane to afford 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(tetrahydro-2H-pyran-4-yl)isoindolin-4-yl)benzene-sulfonamide as an off-white solid (156; 0.15 g, 55% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.83 (bs, 1H), 7.88-7.86 (d, J=8.4 Hz, 2H), 7.74-7.72 (d, J=8.8 Hz, 1H), 7.63-7.59 (m, 3H), 4.13 (m, 1H), 3.94-3.91 (m, 2H), 3.40 (m, 2H), 2.26-2.18 (m, 2H), 1.59-1.57 (m, 2H), 1.30 (s, 9H). MS (M+1): 477.47. (LCMS purity 96.78%, Rt=6.56 min (1)).

Example 26

Synthesis of Compound 157 [4-(tert-butyl)-N-(7-methyl-1,3-dioxo-2-(pyridin-3-yl)isoindolin-4-yl) benzenesulfonamide]; Compound 158 [3-(4-((4-(tert-butyl)phenyl)sulfonamido)-7-methyl-1,3-dioxoisoindolin-2-yl)pyridine 1-oxide] and Compounds 159-169

Synthesis of LXXXI:

To a stirred solution of compound LXXX (1 g, 0.006 mol) in acetic acid (10 mL) was added pyridin-3-amine (II, 0.56 g, 0.006 mol) and the reaction mixture heated at 100° C. for 18 h. This was cooled to room temperature and the acetic acid removed under reduced pressure to obtain the crude product 4-methyl-2-(pyridin-3-yl)isoindoline-1,3-dione as a white solid (LXXXI; 1.1 g,). ¹H NMR (400 MHz, CDCl₃): δ 8.78-8.77 (d, J=2 Hz, 1H), 8.63 (d, J=3.6 Hz, 1H), 7.84-7.80 (m, 2H), 7.69-7.65 (t, J=7.6 Hz, 1H), 7.57-7.55 (d, J=8 Hz, 1H), 7.47-7.43 (m, 1H), 2.76 (s, 3H). MS (M+1): 239.02.

Synthesis of LXXXII:

To a stirred solution of KNO₃(1.9 g, 0.018 mol) in concentrated sulfuric acid (22 mL) at 0° C. was added compound LXXXI (0.9 g, 0.003 mol) in sulfuric acid. The reaction mixture was stirred at room temperature for 1 h and then cooled to 0° C. before the addition of crushed ice. The aqueous layer was extracted with ethyl acetate which was washed with brine solution, dried over anhydrous Na₂SO₄and evaporated under reduced pressure. The solid crude so obtained was purified by column chromatography using 30% ethyl acetate in hexane to afford 4-methyl-7-nitro-2-(pyridin-3-yl)isoindoline-1,3-dione as a white solid (LXXXII; 0.45 g, 45% yield). ¹H NMR (400 MHz, CDCl₃): δ 8.75 (s, 1H), 8.66-8.65 (d, J=4 Hz, 1H), 8.03-8.01 (d, J=8 Hz, 1H), 7.81-7.79 (d, J=7.6 Hz, 1H), 7.74-7.72 (d, J=8 Hz, 1H), 7.48-7.45 (m, 1H), 2.86 (s, 3H). MS (M+1): 284.06

Synthesis of LXXXIII:

To a stirred solution of LXXXII, (0.45 g, 1.59 mmol) in methanol (15 mL) and ethyl acetate (2 mL) under a nitrogen atmosphere was added 10% Pd—C(0.1 g). The reaction mixture was purged with nitrogen and stirred under hydrogen balloon pressure for 5 h at room temperature. The reaction mixture was filtered through a celite bed under a nitrogen atmosphere and evaporated under reduced pressure to afford crude compound 4-amino-7-methyl-2-(pyridin-3-yl)isoindoline-1,3-dione as a yellow solid (LXXXIII; 0.35 g). ¹H NMR (400 MHz, DMSO-d6): δ 8.64-8.63 (d, J=2 Hz, 1H), 8.59-8.58 (d, J=4 Hz, 1H), 7.88-7.86 (d, J=8.4 Hz, 1H), 7.57-7.54 (m, 1H), 7.34-7.32 (d, J=8.4 Hz, 1H), 6.99-6.97 (d, J=8.4 Hz, 1H), 6.45 (bs, 2H), 2.46 (s, 3H). MS (M+1): 254.02.

Synthesis of 157; 4-(tert-butyl)-N-(7-methyl-1,3-dioxo-2-(pyridin-3-yl)isoindolin-4-yl)benzenesulfonamide

To a stirred mixture of compound LXXXIII (0.12 g, 0.47 mmol) in chloroform (30 mL) was added pyridine (3 mL) at 0° C. followed by the addition of 4-tert-butylbenzenesulfonyl chloride (V, 0.22 g, 0.94 mmol). The reaction mixture was stirred at 80° C. for 24 h. The reaction mixture was concentrated at reduced pressure and diluted with water. The aqueous layer was extracted with ethyl acetate and the combined organic layers were washed with brine, dried over Na₂SO₄, filtered and concentrated to leave the crude compound, which was purified by column chromatography using 30% ethyl acetate in hexane to afford 4-(tert-butyl)-N-(7-methyl-1,3-dioxo-2-(pyridin-3-yl)isoindolin-4-yl)benzenesulfonamide (157; 0.07 g; 33% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.76 (bs, 1H), 8.63-8.59 (m, 2H), 7.89-8.83 (m, 3H), 7.63-7.57 (m, 5H), 2.55 (s, 3H), 1.26 (s, 9H). MS (M+1): 450.22. (LCMS purity 99.71%, Rt=4.26 min (1)).

Synthesis of 158; 3-(4-((4-(tert-butyl)phenyl)sulfonamido)-7-methyl-1,3-dioxoisoindolin-2-yl)pyridine 1-oxide

To a stirred solution of compound 157 (0.07 g, 0.22 mmol) in dichloromethane (15 mL) was added meta-chloroperoxybenzoic acid (0.057, 0.33 mmol). The reaction mixture was stirred at room temperature for 24 h and then water was added. The layers were separated and the aqueous layer was extracted with ethyl acetate. The organic layer was washed with sodium bicarbonate, dried (anhydrous Na₂SO₄) and concentrated. The crude mass was triturated with diethyl ether to afford 3-(4-((4-(tert-butyl) phenyl)sulfonamido)-7-methyl-1,3-dioxoisoindolin-2-yl) pyridine 1-oxide (158; 0.05 g; 50% yield). 1H NMR (400 MHz, DMSO-d6): δ 9.80 (bs, 1H), 8.34 (s, 1H), 8.30-8.29 (d, J=6 Hz, 1H), 7.90-7.88 (d, J=8.4 Hz, 2H), 7.62-7.44 (m, 5H), 7.44-7.42 (d, J=8 Hz, 1H), 2.54 (s, 3H), 1.26 (s, 9H). MS (M−1): 464.03. (LCMS purity 98.14%, Rt=5.76 min(1)).
The following compounds were prepared in a similar manner using the appropriate anhydride and/or amine in the first step and/or sulfonyl chloride instead of 4-tert-butylbenzenesulfonyl chloride V in the penultimate step. Products which are not pyridine N-oxides are not taken through the mCPBA oxidation procedure described in the final step of this example 26


		LCMS	Purity
Cpd	Structure	(M + 1)	(LCMS)	¹H NMR

159		462.14	98.48% Rt = 5.83 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.31 (bs, 1H), 8.62-8.61 (d, J = 3.6 Hz, 1H), 8.56-8.55 (d, J = 2 Hz, 1H), 8.12-8.10 (d, J = 8.4 Hz, 2H), 8.00-7.98 (d, J = 8.4 Hz, 2H), 7.81- 7.79 (d, J = 8 Hz, 1H), 7.65-7.63 (d, J = 8.8 Hz, 1H), 7.59-7.53 (m, 2H), 2.57 (s, 3H).

160		464.22	98.68% Rt = 6.62 min (1)	¹H NMR (400MHz, DMSO-d6): δ 9.72 (bs, 1H), 8.53.-8.48 (m, 2H), 7.87-7.85 (d, J = 8 Hz, 2H), 7.70- 7.68 (d, J = 7.6 Hz, 1H), 7.59-7.57 (d, J = 8 Hz, 2H), 7.52-7.51( m, 2H), 7.35 (m, 1H), 4.72 (s, 2H), 3.32 (s, 3H), 1.25 (s, 9H).

161		475.86	98.91% Rt = 5.88 min (1)	1H NMR (400MHz, DMSO-d6): δ 10.25 (bs, 1H), 8.52-8.48 (m, 2H), 8.09-8.07 (d, J = 8 Hz, 2H), 7.95- 7.93 (d, J = 8 Hz, 2H), 7.63-7.61 (d, J = 8 Hz, 1H), 7.56-7.54 (d, J = 8.4 Hz, 1H), 7.46-7.44 (d, J = 8.4 Hz, 1H), 7.35-7.32 (m, 1H), 4.69 (s, 2H), 2.57 (s, 3H).

162		491.94	99.73% Rt = 6.07 min (1)	1H NMR (400MHz, CDCl3): δ 8.91 (s, 1H), 8.66 (s, 1H), 8.54 (bs, 1H), 7.94-7.92 (d, J = 9.2 Hz, 2H), 7.76- 7.74 (d, J = 8.8 Hz, 1H), 7.71-7.69 (d, J = 8 Hz, 1H), 7.39-7.37 (d, J = 8.4 Hz, 1H), 7.28-7.22 (m, 2H), 4.75 (s, 2H), 2.56 (s, 3H).

163		466.20	98.22% Rt = 6.09 min (1)	¹H NMR (400MHz, DMSO- d6-): δ 9.70 (bs, 1H), 8.60-8.59 (d, J = 3.6 Hz, 1H), 8.51 (s, 1H), 7.79-7.74 (m, 3H), 7.68-7.65 (d, J = 9.2 Hz, 1H), 7.60-7.53 (m, 4H), 3.94 (s, 3H), 1.25 (s, 9H).

164		478.13	99.75% Rt = 5.42 min (1)	¹H NMR (400MHz, DMSO- d6-): δ 10.24 (bs, 1H), 8.60-8.59 (dd, J = 1.6 Hz, 3.2 Hz, 1H), 8.47-8.46 (d, J = 2 Hz, 1H), 7.96 (m, 4H), 7.71-7.69 (m, 1H), 7.63-7.60 (d, J = 9.2 Hz, 1H), 7.55-7.53 (m, 2H), 3.95 (s, 3H).

165		494.17	97.42% Rt = 5.63 min (1)	¹H NMR (400MHz, DMSO- d6-): δ 10.07 (bs, 1H), 8.63-8.59 (m, 1H), 8.50-8.49 (d, J = 2 Hz, 1H), 7.91- 7.90 (d, J = 6.8 Hz, 2H), 7.75-7.73 (m, 1H), 7.64-7.58 (m, 5H), 3.89 (s, 3H).

166		480.42	99.67% Rt = 6.24 min (1)	¹H NMR (400MHz, DMSO-d6): δ 9.64 (bs, 1H), 8.48 (m, 2H), 7.76- 7.74 (d, J = 8.4 Hz, 2H), 7.65-7.63 (d, J = 8.0 Hz, 1H), 7.58-7.54 (m, 3H), 7.46-7.44 (d, J = 9.6 Hz, 1H) 7.35-7.33 (m, 1H) 4.66 (s, 2H), 3.88 (s, 3H), 1.24 (s, 9H).

167		508.37	98.89% Rt = 5.81 min (1)	¹H NMR (400MHz, DMSO-d6): δ 9.98 (bs, 1H), 8.47 (s, 2H), 7.88- 7.85 (d, J = 8.8 Hz, 2H), 7.56-7.46 (m, 5H), 7.34-7.31 (m, 1H), 4.62 (s, 2H), 3.90 (s, 3H).

168		491.97	98.77% Rt = 5.13 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.18 (bs, 1H). 8.46 (m, 2H), 7.97- 7.90 (m, 4H), 7.54-7.52 (m, 2H), 7.48-7.46 (m, 1H), 7.33-7.30 (m, 1H), 4.62 (s, 2H), 3.90 (s, 3H).

169		526.21	95.53% Rt = 5.52 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.38 (bs, 1H), 8.47 (m, 2H), 8.16 (s, 1H),7.92-7.85 (m, 2H), 7.53-7.47 (m, 3H), 7.33 (s, 1H), 4.61 (s, 2H), 3.91 (s, 3H).

Example 27

Synthesis of Compound 170 [4-(tert-butyl)-N-(7-cyclopropyl-1,3-dioxo-2-(pyridin-3-yl)isoindolin-4-yl)benzenesulfonamide] and Compounds 171-174

Synthesis of LXXXV:

A stirred solution of 3-chloro-6-nitrophthalic acid (XXIV, 10 g, 36.6 mmol) in DMF (20 mL) was cooled to 0° C. and methyl iodide (13 g, 95 mmol) in DMF solution (16 mL) was added. The reaction mixture was heated at 70° C. for 12 h and on cooling this was diluted with ice cold water. The aqueous layer was extracted with ethyl acetate and the organic layer was dried over anhydrous Na₂SO₄, filtered and evaporated under reduced pressure to afford crude compound dimethyl 3-chloro-6-nitrophthalate (LXXXV, 10 g, crude). ¹H NMR (400 MHz, DMSO-d6): δ 8.08-8.06 (m, 1H), 7.67-7.66 (m, 1H), 3.99 (s, 3H), 3.96 (s, 3H).

Synthesis of LXXXVII:

To a stirred solution of compound LXXXV (10 g, 36 mmol) in dioxane (360 mL) was added cyclopropaneboronic acid (LXXXVI, 6.29 g, 73 mmol). This was followed by a solution of sodium carbonate (11.64 g, 109 mmol) in water (75 mL). The reaction was purged under an argon atmosphere for 30 minutes. The catalyst Pd(dppf)Cl₂(5.9 g, 7.3 mmol) was added to the reaction mixture and the mixture was heated at 120° C. for 12 h. On cooling the solution was filtered through a celite bed. The dioxane was concentrated under reduced pressure and the crude compound was directly purified by column chromatography using 30% ethyl acetate in hexane to afford dimethyl 3-cyclopropyl-6-nitrophthalate (LXXXVII, 2.1 g, 24.5% yield). ¹H NMR (400 MHz, CDCl₃): δ 8.03-8.01 (d, J=8.8 Hz, 1H), 7.15-7.13 (d, J=8.8 Hz, 1H) 3.91 (s, 6H), 2.23 (m, 1H), 1.15-1.13 (m, 2H), 0.82-0.79 (m, 2H).

Synthesis of LXXXVIII:

To a stirred solution of compound LXXXVII (2.1 g, 7.52 mmol) in DME (5 mL) was added a solution of sodium hydroxide (0.6 g, 15.1 mmol) in water (1 mL). The reaction mixture was heated at 70° C. for 48 h. On cooling it was diluted with ice cold water and acidified to pH 2 using 1N HCl solution. The aqueous layer was extracted with ethyl acetate. The organic layer was dried over anhydrous Na₂SO₄, filtered and evaporated under reduced pressure to afford 3-cyclopropyl-6-nitrophthalic acid (LXXXVIII, 1.5 g, 80% yield). ¹H NMR (400 MHz, DMSO-d6): δ 13.9 (bs, 2H), 8.00-7.98 (d, J=8.4 Hz, 1H), 7.25-7.23 (d, J=8.4 Hz, 1H), 2.2 (m, 1H), 1.15 (m, 2H), 0.84 (m, 2H).

Synthesis of LXXXIX:

A stirred solution of compound LXXXVIII (1.5 g; 5.97 mmol) in acetic anhydride (20 mL) was heated at 120° C. for 12 h. The reaction mixture was cooled and diluted with water. The aqueous layer was extracted with ethyl acetate. The organic layer was washed with brine, dried over anhydrous Na₂SO₄, filtered and concentrated to afford 4-cyclopropyl-7-nitroisobenzofuran-1,3-dione as an off-white solid (LXXXIX; 1.2 g, 92.3% yield). MS (M−1): 232.20.

Synthesis of XC:

To a stirred solution of compound LXXXIX (1.2 g, 5.15 mmol) in acetic acid (20 mL) was added pyridin-3-amine (II, 0.91 g, 10.3 mmol). The reaction mixture was heated at 120° C. for 12 h. On cooling to room temperature, the acetic acid was removed under reduced pressure to leave the crude product which was triturated with ethanol to afford 4-cyclopropyl-7-nitro-2-(pyridin-3-yl)isoindoline-1,3-dione (XC, 1.2 g, 86%). MS (M−1): 308.99.

Synthesis of XCI:

To a solution of compound XC (1.2 g, 3.88 mmol) in acetic acid (10 mL) was added iron powder (1 g) in small portions. The reaction mixture was stirred for 12 h at room temperature and then filtered through a celite bed and concentrated under reduced pressure. The crude mass was neutralized by aqueous sodium bicarbonate solution. The aqueous layer was extracted with ethyl acetate. The organic solvent which was dried over anhydrous Na₂SO₄, filtered and evaporated under reduced pressure to afford 4-amino-7-cyclopropyl-2-(pyridin-3-yl)isoindoline-1,3-dione as an off white solid (XCI; 1 g, 92% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.65 (s, 1H), 8.59-8.58 (d, J=4.8 Hz, 1H), 7.90-7.88 (d, J=8.8 Hz, 1H), 7.58-7.55 (m, 1H), 6.98 (m, 2H), 6.44 (bs, 2H), 2.94-2.89 (m, 1H), 1.01-0.97 (m, 2H), 0.77-0.73 (m, 2H).

Synthesis of 170; 4-(tert-butyl)-N-(7-cyclopropyl-1,3-dioxo-2-(pyridin-3-yl)isoindolin-4-yl)benzenesulfonamide

A mixture of compound XCI (0.2 g, 0.72 mmol) and pyridine (3 mL) was cooled to 0° C. and 4-tert-butylbenzenesulfonyl chloride (V, 0.33 g, 1.43 mmol) was added followed by DMAP (0.047 g, 0.35 mmol). The reaction mixture was heated for 12 h at 100° C. The reaction mixture was concentrated and diluted with water. The reaction mixture was extracted with ethyl acetate. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound. This was purified by column chromatography using 40% ethyl acetate in hexane to afford 4-(tert-butyl)-N-(7-cyclopropyl-1,3-dioxo-2-(pyridin-3-yl)isoindolin-4-yl)benzene-sulfonamide as an off-white solid (170; 0.08 g, 23.52% yield). ¹H NMR (400 MHz, DMSO-d6): δ 9.76 (bs, 1H), 8.63-8.61 (m, 2H), 7.87-7.85 (m, 3H), 7.63-7.55 (m, 4H), 7.26-7.24 (d, J=8.8 Hz, 1H), 2.98-2.97 (m, 1H), 1.27 (s, 9H), 1.12 (m, 2H), 0.85 (m, 2H). MS (M+1): 476.11. (LCMS purity 99.32%, Rt=6.73 min (2)).
The following compounds were also prepared using a similar method and the appropriate sulfonyl chloride in the final step described. Conversion to the pyridine N-oxide is achieved by treatment of the corresponding pyridine with mCPBA:


		LCMS	Purity
Cpd	Structure	(M + 1)	(LCMS)	¹H NMR

171		488.36	99.57%, Rt = 6.0 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.31 (bs, 1H), 8.62 (s, 1H), 8.57 (m, 1H), 8.09-8.07 (d, J = 8.0 Hz, 2H), 7.99-7.97 (d, J = 8.4 Hz, 2H), 7.82-7.80 (d, J = 8.4 Hz, 1H), 7.59- 7.56 (d, J = 8.8 Hz, 1H), 7.50-7.48 (d, J = 8.8 Hz, 1H) 7.25-7.23 (d, J = 8.8 Hz, 1H), 3.01 (m, 1H), 1.13 (m, 2H), 0.86 (m, 2H).

172		504.38	99.83%, Rt = 6.20 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.12 (bs, 1H), 8.63-8.59 (m, 2H), 8.04-8.02 (d, J = 8.8 Hz, 2H), 7.84- 7.82 (d, J = 8.0 Hz, 1H), 7.60-7.56 (m, 3H), 7.52-7.50 (d, J = 8.8 Hz, 1H), 7.26-7.24 (d, J = 8.8 Hz, 1H), 3.01 (m, 1H), 1.13 (m, 2H), 0.87 (m, 2H).

173		492.39	96.98%, Rt = 5.97 min (1)	¹H NMR (400MHz, DMSO-d6): δ 9.82 (bs, 1H), 8.36 (s, 1H), 8.30- 8.28 (d, J = 6.4 Hz, 1H), 7.85-7.83 (d, J = 8.4 Hz, 2H), 7.59-7.53 (m, 4H), 7.46-7.44 (d, J = 8.4 Hz, 1H), 7.16 (m, 1H), 2.98-2.92 (m, 1H), 1.27 (s, 9H), 1.10 (m, 2H), 0.87 (s, 2H).

174		518.35 (M − 1)	98.51%, Rt = 5.31 min (1)	¹H NMR (400MHz, DMSO-d6): δ 10.19 (bs, 1H), 8.34 (s, 1H), 8.31- 8.30 (d, J = 6.4 Hz, 1H), 8.06-8.02 (d, J = 8.8 Hz, 2H), 7.61-7.56 (m, 3H), 7.51-7.49 (d, J = 8.8 Hz, 1H), 7.43-7.41 (d, J = 8.4 Hz, 1H), 7.26- 7.24 (d, J = 8.0 Hz, 1H), 2.99 (m, 1H), 1.13-1.12 (m, 2H), 0.86-0.85 (m, 2H).

Example 28

Synthesis of Compound 175 [4-(tert-butyl)-N-(7-chloro-2-(2-methylpyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide]; Compound 176 [3-(4-(4-(tert-butyl)phenylsulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)-2-methylpyridine 1-oxide] and Compounds 177 to 276, and Compounds 277 to 280

Synthesis of XXIV:

To a stirred solution of nitric acid and sulphuric acid (172 ml: 439 ml) at 0° C. was added portion wise compound XCII (250.0 g, 1.37 mol) and the reaction mixture was stirred at room temperature for 12 h. The reaction mixture was cooled to 0° C. followed by addition of crushed ice. The solid which precipitated out was filtered to afford 3-chloro-6-nitrophthalic acid as a white solid (XXIV; 250.0 g; 74% yield). ¹H NMR (400 MHz, DMSO-d6): δ 14.34 (bs, 2H), 8.16-8.13 (d, J=8.8 Hz, 1H), 7.93-7.91 (d, J=8.8 Hz, 1H). MS (M−1): 243.97.

Synthesis of XXV:

A stirred solution of compound XXIV (250.0 g, 1.02 mol) in acetic anhydride (3.5 L) was heated at 120° C. for 18 h. The reaction mixture was cooled and concentrated under reduced pressure. The crude solid obtained was washed with hexane and triturated with 20% diethyl ether in hexane to afford 4-chloro-7-nitroisobenzofuran-1,3-dione as an off white solid (XXV; 230 g; 99% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.48-8.46 (d, J=8.8 Hz, 1H), 8.30-8.27 (d, J=8.8 Hz, 1H). MS (M+1): 228.01.

Synthesis of XCIV:

To a stirred solution of compound XXV (230 g, 1.01 mol) in acetic acid (4 L) was added 2-methylpyridin-3-amine (XCIII, 118.15 g, 1.09 mol) and the reaction mixture heated at 120° C. for 4 h. The reaction mixture was cooled to room temperature and the acetic acid removed under reduced pressure. The residual material was washed with hexane to obtain a crude product 4-chloro-2-(2-methylpyridin-3-yl)-7-nitroisoindoline-1,3-dione as a yellow solid (XCIV; 300 g,). MS (M+1): 318.09. The crude material was carried forward to next step without purification.

Synthesis of XCV:

To a solution of compound XCIV (280 g) in acetic acid (10 L) under a nitrogen atmosphere was added iron powder (280 g). The reaction mixture was stirred for 12 h at room temperature. The reaction mixture was filtered through a celite bed followed which was washed with ethyl acetate and the collected filtrate was evaporated under reduced pressure to afford compound 4-amino-7-chloro-2-(2-methylpyridin-3-yl)isoindoline-1,3-dione as a yellow solid (XCV; 220 g; 86% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.55-8.54 (m, 1H), 7.80-7.78 (d, J=7.6 Hz, 1H), 7.50-7.48 (d, J=8.8 Hz, 1H), 7.41-7.38 (m, 1H), 7.08-7.06 (d, J=8.8 Hz, 1H), 6.73 (s, 2H), 2.34 (s, 3H). MS (M+1): 288.19

Synthesis of 175; 4-(tert-butyl)-N-(7-chloro-2-(2-methylpyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide

A mixture of compound XCV (210 g, 0.731 mol) in pyridine (4 L) was cooled to 0° C. and 4-(tert-butyl) benzenesulfonyl chloride (V, 509 g, 2.19 mol) was added. The reaction mixture was stirred at 90° C. for 8 h. The reaction mixture was cooled and concentrated under reduced pressure. The reaction mixture was diluted with water and the aqueous layer was extracted with ethyl acetate. The organic layer was separated, washed with brine solution, dried over anhydrous Na₂SO₄, filtered and the concentrated under reduced pressure to obtain the crude compound XCVI. To the crude compound XCVI, was added 1M TBAF in THF solution (4 L) and the resulting mixture stirred for 3 h at room temperature. The reaction mixture was concentrated at reduced pressure and diluted with ethyl acetate. The organic layer was washed with water, dried over anhydrous Na₂SO₄, filtered and evaporated under reduced pressure to obtain the crude compound which was purified by column chromatography using 25% ethyl acetate in hexane to afford the title compound, 4-(tert-butyl)-N-(7-chloro-2-(2-methylpyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide as an off white solid (175; 240 g, 68% yield). ¹H NMR (400 MHz, DMS 0-d6): δ 10.00 (bs, 1H), 8.57-8.56 (d, J=3.6 Hz, 1H), 7.92-7.90 (d, J=8.4 Hz, 2H), 7.83-7.81 (d, J=8.8 Hz, 1H), 7.78-7.76 (d, J=7.2 Hz, 1H), 7.70-7.67 (d, J=8.8 Hz, 1H), 7.64-7.61 (d, J=8.4 Hz, 2H), 7.43-7.39 (m, 1H), 2.31 (s, 3H), 1.27 (s, 9H). MS (M+1): 484.03. (LCMS purity 99.02%, Rt=6.06 min (1)).

Synthesis of 176; 3-(4-(4-(tert-butyl)phenylsulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)-2-methylpyridine 1-oxide

To a stirred solution of 4-(tert-butyl)-N-(7-chloro-2-(2-methylpyridin-3-yl)-1,3-dioxoisoindolin-4-yl)benzenesulfonamide (175; 230 g, 0.475 mol) in dichloromethane (3.4 L), was added m-chloroperoxybenzoic acid (81.97 g, 0.475 mol). The reaction mixture was stirred at RT for 8 h whereupon the solvent was concentrated under reduced pressure and the residue was diluted with water. The aqueous layer was extracted with dichloromethane. The combined organic layers were washed sequentially with sodium bicarbonate and brine, then dried over Na₂SO₄, filtered and concentrated under vacuum to leave the crude compound, which was purified by column chromatography using 1% methanol in dichloromethane to afford 3-(4-(4-(tert-butyl)phenylsulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)-2-methylpyridine 1-oxide as an off white solid (176; 125 g, 53% yield). ¹H NMR (400 MHz, DMSO-d6): δ 10.04 (bs, 1H), 8.45-8.43 (d, J=5.6 Hz, 1H), 7.93-7.91 (d, J=8.4 Hz, 2H), 7.85-7.83 (d, J=8.8 Hz, 1H), 7.69-7.67 (d, J=8.8 Hz, 1H), 7.64-7.62 (d, J=8.8 Hz, 2H), 7.47-7.40 (m, 2H), 2.20 (s, 3H), 1.27 (s, 9H). MS (M+1): 500.28. (LCMS purity 99.45%, Rt=5.40 min (1)).
The following compounds were prepared essentially in a similar manner as described above using the appropriate amine instead of 2-methylpyridin-3-amine XCIII. The final step described was only carried out only for those compounds where a pyridine-N-oxide was produced:


S.		LCMS	Purity
No.	Structure	(M + 1)	(LCMS)	1HNMR

177		495.06	99.87% Rt = 5.98 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.09 (bs, 1H), 9.12-9.11 (d, J = 1.6 Hz, 1H), 8.93-8.92 (d, J = 2 Hz, 1H), 8.38-8.09 (m, 1H), 7.94-7.92 (d, J = 8.8 Hz, 2H), 7.88-7.86 (d, J = 8.8 Hz, 1H), 7.71-7.69 (d, J = 8.8 Hz, 1H), 7.65-7.63 (d, J = 8.8 Hz, 2H), 1.27 (s, 9H).

178		476.00	97.32% Rt = 6.03 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.12 (bs, 1H), 9.06 (s, 1H), 8.21 (s, 1H), 7.94-7.92 (d, J = 8.8 Hz, 2H), 7.83-7.81 (d, J = 8.8 Hz, 1H), 7.66-7.62 (m, 3 H), 1.26 (s, 9H).

179		497.92 (M − 1)	98.42% Rt = 6.20 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.20 (bs, 1H), 7.89-7.77 (m, 5H), 7.62-7.60 (d, J = 8.4 Hz, 2H), 7.47-7.46 (d, J = 5.2 Hz, 1H), 1.30 (s, 9H).

180		516.03	97.29% Rt = 5.15 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.04 (bs, 1H), 8.35-8.34 (d, J = 2 Hz, 1H), 7.94-7.92 (d, J = 8 Hz, 2H), 7.80 (m, 1H), 7.68-7.62 (m, 3H), 7.44-7.43 (d, J = 2 Hz, 1H), 7.37-7.35 (d, J = 8.8 Hz, 1H), 4.03 (s, 3H), 1.27 (s, 9H).

181		476.09	99.78% Rt = 5.79 min (2)	¹H NMR (400MHz, DMSO-d6 with TFA): δ 9.65 (bs, 1H), 8.36 (bs, 1H), 7.85-7.83 (d, J = 8.4 Hz, 2H), 7.61 (m, 2H), 7.55-7.53 (d, J = 8.4 Hz, 2H), 4.26 (m, 1H), 3.36 (m, 2H), 3.04 (m, 2H), 2.42 (m, 2 H), 1.83 (m, 2H), 1.21 (s, 9H).

182		484.06	98.42% Rt = 6.21 min (1)	¹H NMR (400MHz, DMSO- d6): δ9.97 (bs, 1H), 8.46-8.45 (d, J = 2Hz, 1H), 7.93-7.91 (d, J = 8.4 Hz, 2H),7.82-7.80 (d, J = 8.8 Hz, 1H), 7.72-7.62 (m, 4H), 7.44-7.42 (d, J = 8 Hz, 1H), 2.53 (s, 3H), 1.27 (s, 9H).

183		500.26	99.56% Rt = 6.40 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.09 (bs, 1H), 8.33-8.31 (m, 1H), 7.94-7.92 (d, J = 8.4 Hz, 2H), 7.82-7.80 (m, 2H), 7.67-7.63 (m, 3H), 7.21-7.18 (m, 1H), 3.84 (s, 3H), 1.28 (s, 9H).

184		471.03	98.49% Rt = 5.94 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.09 (bs, 1H), 8.79-8.78 (m, 3H), 7.93-7.91 (d, J = 8.4 Hz, 2H), 7.88-7.85 (d, J = 9.2 Hz, 1H), 7.70-7.68 (d, J = 8.8 Hz, 1H), 7.64-7.62 (d, J = 8.4 Hz, 2H), 1.27 (s, 9H).

185		484.06	99.08% Rt = 6.13 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.02 (bs, 1H), 8.53-8.52 (d, J = 5.2 Hz, 1H), 8.47 (s, 1H), 7.92-7.90 (d, J = 8.8 Hz, 2H), 7.85-7.83 (d, J = 8.8 Hz, 1H), 7.70-7.68 (d, J = 8.8 Hz, 1H), 7.64-7.62 (d, J = 8.8 Hz, 2H),7.47-7.46 (d, J = 5.6 Hz, 1H), 2.16 (s, 3H), 1.27 (s, 9H).

186		490.15	98.26% Rt = 5.83 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.54 (bs, 1H), 7.80-7.81 (d, J = 8 Hz, 2H), 7.55-7.45 (m, 4H), 4.15-4.12 (m, 2 H), 3.40-3.30 (m, 2H), 2.98-2.91 (m, 1H), 2.67 (s, 3H), 2.43- 2.40 (m, 2H), 1.84-1.80 (m, 2H), 1.27 (s, 9H).

187		488.04	98.06% Rt = 6.30 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.03 (bs, 1H), 8.29 (s, 1H), 8.07-8.04 (m, 1H), 7.94- 7.92 (d, J = 8.8 Hz, 2H),7.84- 7.82 (d, J = 8.4 Hz, 1H), 7.69- 7.67 (d, J = 8.8 Hz, 1H), 7.65- 7.63 (d, J = 8 Hz, 2H), 7.43- 7.40 (m, 1 H), 2H), 1.27 (s, 9H).

188		500.04	96.11% Rt = 5.29 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.08 (bs, 1H), 8.32 (s, 1H), 8.27-8.25 (d, J = 6.4 Hz, 1H), 7.93-7.91 (d, J = 8.4 Hz, 2H),7.86-7.84 (d, J = 8.8 Hz, 1H), 7.70-7.68 (d, J = 8.8 Hz, 1H), 7.64-7.62 (d, J = 8.4 Hz, 2H), 7.48-7.46 (d, J = 6.4 Hz, 1H), 2.11 (s, 3H), 1.27 (s, 9H).

189		516.28	99.14% Rt = 5.13 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.17 (bs, 1H), 8.35-8.33 (m, 1H), 8.31-8.30 (d, J = 2 Hz, 1H), 7.92-7.90 (d, J = 8 Hz, 2H), 7.80 (m, 1H), 7.66- 7.61 (m, 3H), 7.34-7.32 (d, J = 7.2 Hz, 1H), 3.84 (s, 3H), 1.28 (s, 9H).

190		522.03 (M − 1)	99.60% Rt = 6.53 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.0 (bs, 1H), 7.89-7.86 (m, 2H), 7.78 (m, 1H), 7.68- 7.60 (m, 1H), 7.61-7.56 (m, 3H), 7.45 (m, 1H), 7.37 (m, 1H), 3.85 (s, 3H), 1.27 (s, 9H).

191		484.07	99.45% Rt = 6.36 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.00 (bs, 1H), 8.43-8.42 (d, J = 4 Hz, 1H), 7.93-7.90 (d, J = 8.4 Hz, 2H), 7.79-7.75 (m, 2H), 7.63-7.61 (m, 3H), 7.42- 7.40 (d, J = 8 Hz, 1H), 7.29- 7.26 (m, 1H), 4.84 (s, 2H), 1.27 (s, 9H).

192		473.05	96.83% Rt = 5.60 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.15 (bs, 1H), 7.93-7.91 (d, J = 8.8 Hz, 2H), 7.82-7.80 (m, 2H), 7.67-7.61 (m, 3H), 6.97 (s, 1H), 3.38 (s, 3H), 1.27 (s, 9H).

193		487.10	98.75% Rt = 6.89 min (2)	¹H NMR (400MHz, DMSO- d6): δ 9.99 (bs, 1H), 7.91-7.89 (d, J = 7.6 Hz, 2H), 7.74-7.72 (d, J = 8.8 Hz, 1H), 7.62-7.58 (m, 4H), 6.11 (s, 1H), 4.62 (s, 2H), 3.73 (s, 3H), 1.26 (s, 9H).

194		516.27	98.38% Rt = 5.36 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.14 (bs, 1H), 8.42- 8.41 (d, J = 6.4 Hz, 1H), 7.93- 7.91 (d, J = 8.4 Hz, 2H), 7.85- 7.84 (d, J = 1.2 Hz, 1H), 7.69- 7.62 (m, 3H), 7.47-7.45 (d, J = 8 Hz, 1H), 7.37 (m, 1H), 3.98 (s, 3H), 1.27 (s, 9H).

195		509.12	99.14% Rt = 6.92 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.92 (bs, 1H), 8.94 (s, 1H), 8.88 (s, 1H), 8.30 (s, 1H), 7.94-7.92 (d, J = 8.2 Hz. 2H), 7.75-7.73 (d, J = 8.8 Hz, 1H), 7.63-7.59 (m, 3H), 4.82 (s, 2H), 1.26 (s, 9H).

196		512.11	99.02% Rt = 6.62 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.91 (bs, 1H), 8.35-8.34 (d, J = 4 Hz, 1H), 8.29 (s, 1H), 7.87-7.85 (d, J = 8 Hz, 2H), 7.71-7.68 (d, J = 8.8 Hz, 1H), 7.63-7.61 (d, J = 8 Hz, 2H), 7.58-7.56 (d, J = 8.8 Hz, 1H), 7.53-7.51 (d, J = 8.8 Hz, 1H), 7.24-7.21 (m, 1H), 4.42 (m, 1 H), 3.18-3.12 (m, 1H), 3.05- 3.01 (m, 1H), 1.43-1.41 (d, J = 6.8 Hz, 3H), 1.26 (s, 9H).

197		530.26	99.63% Rt = 5.53 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.08 (bs, 1H), 8.17 (s, 1H), 7.99 (s, 1H), 7.95-7.93 (d, J = 8.4 Hz, 2H), 7.86-7.83 (d, J = 8.8 Hz, 1H), 7.69-7.67 (d, J = 8.8 Hz, 1H), 7.67-7.63 (m, 2H), 7.13-7.12 (m, 1H), 4.15- 4.10 (q, J = 7.2 Hz, 6.8 Hz, 2H), 1.35-1.32 (t, 6.8 Hz, 3H), 1.27 (s, 9H).

198		525.08	98.96% Rt = 6.44 min (2)	¹H NMR (400MHz, DMSO- d6): δ 9.91 (bs, 1H), 8.79 (s, 1H), 8.60 (s, 1H), 7.95-7.92 (d, J = 8.4 Hz, 2H), 7.84 (s, 1H), 7.75-7.73 (d, J = 8 Hz, 1H), 7.63-7.59 (m, 3H), 4.73 (s, 2H), 1.27 (s, 9H).

199		500.04	99.22% Rt = 5.51 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.97 (bs, 1H), 8.34-8.32 (d, J = 6.4 Hz, 1H), 7.95-7.93 (d, J = 8.4 Hz, 2H), 7.78-7.76 (d, J = 8.8 Hz, 1H), 7.64-7.61 (m, 3H), 7.51-7.49 (d, J = 7.2 Hz, 1H),7.42-7.39 (m, 1H), 7.31-7.28 (m, 1H), 4.77 (s, 2H), 1.27 (s, 9H).

200		495.05	96.34% Rt = 6.17 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.07 (bs, 1H), 8.93- 8.91 (d, J = 4.8 Hz, 1H), 8.04- 8.03 (d, J = 4.8 Hz, 1H), 7.95- 7.90 (m, 3H), 7.87-7.85 (d, J = 8.8 Hz, 1H), 7.70-7.68 (d, J = 8.8 Hz, 1H), 7.64-7.62 (d, J = 8.4 Hz, 2H), 1.27 (s, 9H).

201		484.08	95.90% Rt = 6.16 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.98 (bs, 1H), 8.51-8.50 (d, J = 5.2 Hz, 2H), 7.93-7.91 (d, J = 8.4 Hz, 2H), 7.77-7.74 (d, J = 8.8 Hz, 1H), 7.63-7.59 (m, 3H), 7.33-7.32 (d, J = 5.2 Hz, 2H), 4.74 (s, 2 H), 1.27 (s, 9H).

202		500.08	98.26% Rt = 6.27 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.10 (bs, 1H), 8.20- 8.19 (d, J = 4 Hz, 1H), 7.91- 7.84 (m, 3H), 7.76-7.74 (d, J = 8 Hz, 1H), 7.68-7.66 (d, J = 8.4 Hz, 1H), 7.64-7.59 (m, 3H), 3.80 (s, 3H), 1.27 (s, 9H).

203		471.07	99.51% Rt = 5.70 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.10 (bs, 1H), 9.37- 9.36 (d, J = 3.6 Hz, 1H), 8.01- 7.98 (m, 1H), 7.94-7.92 (d, J = 8.4 Hz, 2H), 7.87-7.85 (d, J = 8.4 Hz, 2H), 7.71-7.68 (d, J = 9.2 Hz, 1H), 7.65-7.63 (d, J = 8 Hz, 2H), 1.27 (s, 9H).

204		485.07	99.82% Rt = 6.20 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.06 (bs, 1H), 8.75- 8.74 (m, 2H), 7.92-7.90 (d, J = 8.4 Hz, 2H), 7.81-7.79 (d, J = 8.8 Hz, 1H), 7.64-7.61 (m, 3H), 7.45-7.42 (m, 1H), 4.94 (s, 2H), 1.27 (s, 9H).

205		500.08	99.31% Rt = 5.45 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.04 (bs, 1H), 8.37 (s, 1H), 7.94-7.92 (d, J = 8 Hz, 2H), 7.80 (m, 1H), 7.68-7.62 (m, 4H), 7.36-7.34 (d, J = 8 Hz, 1H), 2.40 (s, 3H), 1.27 (s, 9H).

206		514.05	99.53% Rt = 6.71 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.97 (bs, 1H), 8.16-8.15 (d, J = 2 Hz, 1H), 7.94-7.91 (d, J = 8.4 Hz, 2H), 7.82-7.80 (d, J = 9.2 Hz, 1H), 7.73-7.70 (dd, 6.6 Hz, 2.4 Hz, 1H), 7.68-7.63 (m, 3H), 6.97-6.94 (d, J = 8.4 Hz, 1H), 4.37-4.32 (q, J = 6.8 Hz, 7.2 Hz, 2H), 1.36-1.32 (t, J = 6.8 Hz, 3H), 1.27 (s, 9H).

207		495.08	99.47% Rt = 6.24 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.07 (bs, 1H), 8.35- 8.31 (m, 1H), 8.20-8.18 (d, J = 7.2 Hz, 1H), 7.92-7.90 (d, J = 8.4 Hz, 2H), 7.86-7.84 (d, 8.4 Hz, 2H), 7.70-7.68 (d, J = 8.4 Hz, 1H), 7.64-7.62 (d, J = 8.8 Hz, 2H), 1.27 (s, 9H).

208		498.38	99.26% Rt = 6.29 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.98 (bs, 1H), 8.41 (s, 1H), 8.35-8.33 (d, J = 5.2 Hz, 1H), 7.91-7.89 (d, J = 8.4 Hz, 2H), 7.74-7.72 (d, J = 9.2 Hz, 1H), 7.62-7.58 (m, 3H), 7.22- 7.21 (d, J = 4.8 Hz, 1H), 4.72 (s, 2H), 2.36 (s, 3H), 1.26 (s, 9H).

209		500.05	95.09% Rt = 5.35 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.94 (bs, 1H), 8.16-8.14 (d, J = 6.8 Hz, 2H), 7.93-7.91 (d, J = 8.4 Hz, 2H), 7.76-7.74 (d, J = 8.8 Hz, 1H), 7.63-7.58 (m, 3H), 7.37-7.35 (d, J = 6.8 Hz, 2H), 4.68 (s, 2H), 1.26 (s, 9H).

210		530.09	97.07% Rt = 5.28 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.03 (bs, 1H), 8.34- 8.33 (d, J = 2 Hz, 1H), 7.95- 7.93 (d, J = 8.4 Hz, 2H), 7.83- 7.81 (d, J = 8.8 Hz, 1H), 7.68- 7.63 (m, 3H), 7.41-7.34 (m, 2H), 4.35-4.30 (q, J = 6.8 Hz, 7.2 Hz, 2H), 1.42-1.39 (t, J = 7.2 Hz, 3H), 1.27 (s, 9H).

211		516.18	99.11% Rt = 6.46 min (2)	¹H NMR (400MHz, DMSO- d6): δ 10.27 (bs, 1H), 8.16- 8.15 (d, J = 6 Hz, 1H), 7.92- 7.90 (m, 3H), 7.69-7.57 (m, 4H), 7.35-7.33 (d, J = 8.8 Hz, 1H), 3.87 (s, 3H), 1.28 (s, 9H).

212		514.05	99.02% Rt = 6.30 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.93 (bs, 1H), 8.40 (s, 1H), 8.34 (s, 1H), 7.93-7.91 (d, J = 8.4 Hz, 2H), 7.76-7.74 (d, J = 9.2 Hz, 1H), 7.70 (m, 1H), 7.63-7.61 (m, 3H), 4.81 (s, 2H), 3.88 (s, 3H), 1.26 (s, 9H).

213		514.05	98.22% Rt = 5.50 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.93 (bs, 1H), 8.14 (s, 1H), 8.05-8.03 (d, J = 6.8 Hz, 1H), 7.93-7.91 (d, J = 8.8 Hz, 2H), 7.74-7.72 (d, J = 8.8 Hz, 1H), 7.63-7.58 (m, 3H), 7.25- 7.23 (d, J = 6.8 Hz, 1H), 4.65 (s, 2H), 2.31 (s, 3H), 1.26 (s, 9H).

214		500.04	97.63% Rt = 6.33 min (2)	¹H NMR (400MHz, DMSO- d6): δ 10.05 (bs, 1H), 8.25 (s, 1H), 8.17 (s, 1H), 7.94-7.92 (d, J = 8.4 Hz, 2H), 7.85-7.83 (d, J = 8.8 Hz, 1H), 7.69-7.67 (d, J = 8.8 Hz, 1H), 7.65-7.63 (d, J = 8.4 Hz, 2H), 7.28 (s, 1H), 2.31 (s, 3H), 1.27 (S, 9H).

215		498.07	99.77% Rt = 6.34 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.94 (bs, 1H), 8.41 (s, 1H), 7.90-7.88 (d, J = 8.8 Hz, 2H), 7.73-7.71 (d, J = 8.8 Hz, 1H), 7.61-7.57 (m, 4H), 7.21- 7.19 (d, J = 8 Hz, 1H),4.68 (s, 2H), 2.42 (s, 3H), 1.26 (s, 9H).

216		485.05	99.04% Rt = 6.0 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.02 (bs, 1H), 8.86- 8.84 (d, J = 5.2 Hz, 1H), 7.88- 7.86 (m, 3H), 7.69-7.67 (d, J = 9.2 Hz, 1H), 7.62-7.57 (m, 3H), 2.55 (s, 3H), 1.26 (s, 9H).

217		490.00	96.66% Rt = 4.72 min (2)	¹H NMR (400MHz, DMSO- d6): δ 10.08 (bs, 1H), 7.93- 7.91 (d, J = 8 Hz, 2H), 7.83- 7.81 (d, J = 8.8 Hz, 1H), 7.67- 7.62 (m, 3H), 7.52 (s, 1H), 2.45 (s, 3H), 1.27 (s, 9H).

218		485.97 (M − 1)	99.36% Rt = 6.20 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.10 (bs, 1H), 8.39- 8.38 (d, J = 2.8 Hz, 1H), 8.12- 8.10 (m, 1H), 7.91-7.89 (d, J = 8.4 Hz, 2H), 7.87-7.84 (d, J = 9.2 Hz, 1H), 7.72-7.69 (m, 1H), 7.63-7.58 (m, 3H), 1.27 (s, 9H).

219		488.02	98.21% Rt = 6.27 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.98 (bs, 1H), 8.66 (m, 1H), 8.01-7.74 (m, 4H), 7.69- 7.53 (m, 4H), 1.27 (s, 9H).

220		485.04	99.85% Rt = 6.06 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.07 (bs, 1H), 8.69 (s, 1H), 8.57 (s, 1H), 7.92-7.90 (d, J = 8.4 Hz, 2H), 7.86-7.84 (d, J = 8.4 Hz, 1H), 7.70-7.68 (d, J = 8.4 Hz, 1H), 7.64-7.62 (d, J = 8.4 Hz, 2H), 2.56 (s, 3H), 1.27 (s, 9H).

221		514.05	98.39% Rt = 5.55 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.91 (bs, 1H), 8.30 (s, 1H), 7.93-7.90 (d, J = 8.4 Hz, 2H), 7.75-7.73 (d, J = 8.8 Hz, 1H), 7.63-7.58 (m, 3H), 7.44- 7.42 (d, J = 8 Hz, 1H),7.22- 7.20 (d, J = 7.6 Hz, 1H), 4.67 (s, 2H), 2.31 (s, 3H), 1.26 (s, 9H).

222		470.02	97.71% Rt = 6.12 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.98 (bs, 1H), 8.64-8.63 (d, J = 4.4 Hz, 1H), 8.06-8.02 (m, 1H), 7.93-7.90 (d, J = 8.4 Hz, 2H), 7.84-7.82 (d, J = 9.2 Hz, 1H), 7.69-7.67 (d, J = 8.8 Hz, 1H), 7.64-7.62 (d, J = 8.4 Hz, 2H), 7.55-7.49 (m, 2H), 1.27 (s, 9H).

223		532.02	92.58% Rt = 7.07 min (2)	¹H NMR (400MHz, DMSO- d6): δ 10.17 (bs, 1H), 7.93- 7.91 (d, J = 8 Hz, 2H), 7.81- 7.77 (d, J = 9.2 Hz, 1H), 7.70- 7.67 (d, J = 8.8 Hz, 1H), 7.63- 7.61 (d, J = 8 Hz, 2H), 2.67 (s, 3H), 2.57 (s, 3H), 1.27 (s, 9H).

224		498.07	99.02% Rt = 6.22 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.99 (bs, 1H), 7.92-7.90 (d, J = 8.4 Hz, 2H), 7.83-7.81 (d, J = 8.8 Hz, 1H), 7.69-7.66 (d, J = 9.2 Hz, 1H), 7.64-7.62 (m, 3H), 7.26-7.24 (d, J = 8 Hz, 1H), 2.48 (s, 3H), 2.24 (s, 3H), 1.27 (s, 9H).

225		484.06	97.84% Rt = 6.29 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.97 (bs, 1H), 8.48-8.47 (d, J = 4.8 Hz, 1H), 7.91-7.89 (d, J = 8.4 Hz, 2H), 7.84-7.82 (d, J = 8.4 Hz, 1H), 7.69-7.67 (d, J = 8.8 Hz, 1H), 7.64-7.62 (d, J = 8.4 Hz, 2H), 7.38-7.36 (d, J = 4.8 Hz, 1H), 7.32 (s, 1H), 2.40 (s, 3H), 1.27 (s, 9H).

226		484.06	99.58% Rt = 6.32 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.97 (bs, 1H), 8.46 (s, 1H), 7.92-7.90 (d, J = 8.4 Hz, 2H), 7.86-7.81 (m, 2H), 7.68- 7.66 (d, J = 8.8 Hz, 1H), 7.64- 7.62 (d, J = 8.4 Hz, 2H), 7.39- 7.36 (d, J = 8 Hz, 1H), 2.38 (s, 3H), 1.27 (s, 9H).

227		514.06	99.30% Rt = 5.57 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.02 (bs, 1H), 7.93- 7.91 (d, J = 8.4 Hz, 2H), 7.84- 7.82 (d, J = 8.8 Hz, 1H), 7.68- 7.62 (m, 3H), 7.54-7.52 (d, J = 8.4 Hz, 1H), 7.33-7.31 (d, J = 8.8 Hz, 1H), 2.45 (s, 3H), 2.22 (s, 3H) 1.28 (s, 9H).

228		484.06	99.87% Rt = 6.32 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.98 (bs, 1H), 7.93-7.91 (m, 3H), 7.83-7.81 (d, J = 8.4 Hz, 1H), 7.68-7.66 (d, J = 8.4 Hz, 1H), 7.64-7.62 (d, J = 8.4 Hz, 2H), 7.40-7.39 (d, J = 7.2 Hz, 1H), 7.30-7.28 (d, J = 7.2 Hz, 1H), 2.45 (s, 3H), 1.27 (s, 9H).

229		500.05	98.18% Rt = 5.50 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.18 (bs, 1H), 8.40 (s, 1H), 7.94-7.92 (d, J = 8.4 Hz, 2H), 7.89-7.86 (d, J = 9.2 Hz, 1H), 7.68-7.62 (m, 4H), 7.37- 7.35 (d, J = 8.4 Hz, 1H), 2.33 (s, 3H), 1.28 (s, 9H).

230		530.06	97.32% Rt = 6.74 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.04 (bs, 1H), 7.94- 7.92 (d, J = 8.4 Hz, 2H), 7.83- 7.81 (d, J = 8.8 Hz, 1H), 7.68- 7.62 (m, 4H), 6.55-6.53 (d, J = 8.4 Hz, 1H), 3.92 (s, 3H), 3.84 (s, 3H), 1.28 (s, 9H).

231		530.09	97.23% Rt = 5.59 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.91 (bs, 1H), 7.98-7.88 (m, 4H), 7.72-7.54 (m, 4H), 6.97 (s, 1H), 4.64 (s, 2H), 3.79 (s, 3H), 1.27 (s, 9H).

232		498.00 (M − 1)	99.31% Rt = 6.22 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.11 (bs, 1H), 8.19- 8.17 (d, J = 5.6 Hz, 1H), 7.93- 7.91 (d, J = 8.4 Hz, 2H), 7.86- 7.84 (d, J = 8.4 Hz, 1H), 7.70- 7.68 (d, J = 8.4 Hz, 1H), 7.64- 7.62 (d, J = 8.4 Hz, 2H), 7.30- 7.29 (d, J = 5.2 Hz, 1H), 1.27 (s, 9H).

233		500.05	97.58% Rt = 6.59 min (2)	¹H NMR (400MHz, DMSO- d6): δ 10.15 (bs, 1H), 7.93- 7.87 (m, 3H), 7.67-7.63 (m, 5H), 7.43 (m, 1H), 2.40 (s, 3H), 1.28 (s, 9H).

234		473.05 (M − 1)	96.42% Rt = 5.83 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.14 (bs, 1H), 7.91- 8.85 (m, 3H), 7.69-7.63 (m, 3H), 4.49 (s, 3H), 1.27 (s, 9H).

235		514.09	99.34% Rt = 4.76 min (2)	¹H NMR (400MHz, DMSO- d6): δ9.91 (bs, 1H), 8.13 (s, 1H), 7.90-7.88 (d, J = 8.4 Hz, 2H), 7.76-7.71 (m, 1H), 7.65- 7.57 (m, 4H), 6.78-6.76 (d, J = 8.4 Hz, 1H), 4.64 (s, 2H), 3.81 (s, 3H), 1.25 (s, 9H).

236		485.05	98.73% Rt = 6.64 min (2)	¹H NMR (400MHz, DMSO- d6): δ 10.10 (bs, 1H), 7.94-7.92 (d, J = 8.4 Hz, 2H), 7.85-7.83 (d, J = 8.4 Hz, 2H), 7.74-7.62 (m, 4H), 2.71 (s, 3H), 1.27 (s, 9H).

237		514.30	98.28% Rt = 6.52 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.12 (bs, 1H), 8.18- 8.16 (d, J = 5.2 Hz, 1H), 7.92- 7.89 (d, J = 8.4 Hz, 2H), 7.87- 7.85 (d, J = 8.4 Hz, 1H), 7.69- 7.67 (d, J = 8.4 Hz, 1H), 7.63- 7.61 (d, J = 8.4 Hz, 2H), 7.08- 7.07 (d, J = 5.2 Hz, 1H), 3.78 (s, 3H), 2.12 (s, 3 H), 1.27 (s, 9H).

238		514.29	97.64% Rt = 6.64 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.98 (bs, 1H), 7.92-7.90 (d, J = 8.4 Hz, 2H), 7.83-7.81 (d, J = 8.4 Hz, 1H), 7.68-7.57 (m, 4H), 6.80-6.78 (d, J = 8.4 Hz, 1H), 3.88 (s, 3H), 2.20 (s, 3H), 1.27 (s, 9H).

239		502.24	98.77% Rt = 6.51 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.07 (bs, 1H), 8.28- 8.26 (d, J = 4.4 Hz, 1H), 7.92- 7.90 (d, J = 8.4 Hz, 2H), 7.80- 7.73 (m, 2H), 7.63-7.70 (m, 3H), 7.43-7.41 (m, 1H), 4.93 (s, 2H), 1.27 (s, 9H).

240		499.27	99.13% Rt = 6.13 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.04 (bs, 1H), 7.89- 7.84 (m, 3H), 7.69-7.67 (d, J = 9.2 Hz, 1H), 7.63-7.61 (d, J = 8 Hz, 2H), 7.45 (s, 1H), 2.50 (s, 6H), 1.27 (s, 9H).

241		518.30	97.98% Rt = 5.82 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.00 (bs, 1H), 8.15- 8.13 (d, J = 6.4 Hz, 1H),7.86 (m, 2H), 7.70-7.54 (m, 4H), 7.47-7.37 (m, 2H), 4.87 (s, 2H), 1.27 (s, 9H).

242		514.34	97.43% Rt = 6.21 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.01 (bs, 1H),8.38 (s, 1H), 8.16 (s, 1H),7.91-7.89 (d, J = 8.4 Hz, 2H), 7.83-7.81 (d, J = 8.8 Hz, 1H), 7.69-7.58 (m, 3H), 3.97 (s, 3H), 1.97 (s, 3H), 1.27 (s, 9H).

243		530.29	97.04% Rt = 5.22 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.02 (bs, 1H), 7.91- 7.89 (d, J = 8 Hz, 2H), 7.75 (m, 1H), 7.66-7.60 (m, 3H), 7.41- 7.39 (d, J = 9.2 Hz, 1H), 7.26- 7.23 (d, J = 9.2 Hz, 1H), 4.03 (s, 3H), 2.19 (s, 3H), 1.27 (s, 9H).

244		484.31	99.45% Rt = 6.03 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.03 (bs, 1H), 8.64 (s, 1H), 8.56-8.55 (d, J = 5.2 Hz, 1H), 7.92-7.90 (d, J = 8.4 Hz, 2H),7.85-7.82 (d, J = 9.2 Hz, 1H), 7.70-7.67 (d, J = 8.8 Hz, 1H),7.64-7.62 (d, J = 8.4 Hz, 2H),7.39-7.37 (d, J = 5.2 Hz, 1H), 2.14 (s, 3H), 1.27 (s, 9H).

245		530.35	96.18% Rt = 5.53 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.07 (bs, 1H), 8.19 (s, 1H), 8.05 (s, 1H), 7.93-7.91 (d, J = 8.4 Hz, 2H),7.84-7.82 (d, J = 8.4 Hz, 1H), 7.69-7.66 (d, J = 8.8 Hz, 1H),7.64-7.62 (d, J = 8.4 Hz, 2H), 3.91 (s, 3H), 1.92 (s, 3H),1.27 (s, 9H).

246		542.39	97.64% Rt = 4.87 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.88 (bs, 1H), 7.91-7.88 (d, J = 8.4 Hz, 2H), 7.76-7.74 (d, J = 8.4 Hz, 1H), 7.62-7.60 (m, 3H), 7.46-7.45 (d, J = 7.2 Hz, 1H), 6.76-6.74 (d, J = 7.6 Hz, 1H), 4.59 (s, 2H), 4.28- 4.22 (q, J = 7.2 Hz, 6.8 Hz, 2H), 2.33 (s, 3H), 1.26 (s, 9H), 1.20-1.17 (t, J = 6.8 Hz, 3H).

247		514.34	98.46% Rt = 6.71 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.94 (bs, 1H), 8.09 (m, 1H),7.92-7.90 (d, J = 8.4 Hz, 2H),7.76-7.74 (d, J = 8.4 Hz, 1H), 7.63-7.60 (m, 3H), 7.56- 7.55 (d, J = 6.4 Hz, 1H), 6.93- 6.90 (m, 1H), 4.62 (s, 2H), 3.88 (s, 3H), 1.26 (s, 9H).

248		512.32	99.27% Rt = 6.38 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.99 (bs, 1H), 7.91-7.89 (d, J = 8.4 Hz, 2H),7.74-7.72 (d, J = 9.2 Hz, 1H), 7.61-7.58 (m, 3H), 7.48-7.46 (d, J = 7.6 Hz, 1H), 7.03-7.01 (d, J = 8 Hz, 1H), 4.66 (s, 2H), 2.49 (s, 3H), 2.38 (s, 3H), 1.26 (s, 9H).

249		471.26	96.36% Rt = 5.64 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.09 (bs, 1H), 9.29 (s, 1H), 9.05-9.03 (d, J = 5.6 Hz, 1H), 7.94-7.91 (d, J = 8.4 Hz, 2H),7.87-7.84 (d, J = 8.8 Hz, 1H), 7.70-7.68 (d, J = 8.8 Hz, 1H), 7.65-7.61 (m, 3H), 1.26 (s, 9H).

250		471.28	99.25% Rt = 5.81 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.06 (bs, 1H), 9.04- 9.03 (d, J = 4.8 Hz, 2H), 7.90- 7.84 (m, 3H),7.71-7.67 (m, 2H), 7.63-7.61 (d, J = 8.4 Hz, 2H), 1.26 (s, 9H).

251		498.34	99.72% Rt = 6.52 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.98 (bs, 1H), 8.26 (s, 1H), 7.92-7.90 (d, J = 8.4 Hz, 2H),7.78-7.76 (d, J = 8.8 Hz, 1H), 7.62-7.60 (m, 3H), 7.59- 7.57 (d, J = 8 Hz, 1H), 7.30- 7.29 (d, J = 7.6 Hz, 1H), 4.79 (s, 2H), 2.24 (s, 3H), 1.26 (s, 9H).

252		528.35	99.79% Rt = 5.70 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.95 (bs, 1H), 7.92-7.90 (d, J = 8.4 Hz, 2H),7.76-7.73 (d, J = 8.8 Hz, 1H), 7.62-7.58 (m, 3H), 7.30-7.28 (d, J = 8 Hz, 1H), 7.15-7.13 (d, J = 8.4 Hz, 1H), 4.72 (s, 2H), 2.45 (s, 3H), 2.33 (s, 3H), 1.26 (s, 9H).

253		528.35	99.49% Rt = 5.44 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.92 (bs, 1H), 7.92-7.90 (d, J = 8 Hz, 2H), 7.76-7.74 (d, J = 8.8 Hz, 1H), 7.62-7.59 (m, 3H), 7.43-7.42 (d, J = 7.6 Hz, 1H), 6.77-6.75 (d, J = 7.2 Hz, 1H), 4.58 (s, 2H), 3.91 (s, 3H), 2.36 (s, 3H), 1.26 (s, 9H).

254		498.32	97.29% Rt = 6.20 min (2)	¹H NMR (400MHz, DMSO- d6): δ 9.90 (bs, 1H), 7.92-7.90 (d, J = 8.4 Hz, 2H), 7.78-7.75 (d, J = 9.2 Hz, 1H), 7.63-7.57 (m, 4H), 7.14-7.13 (d, J = 6 Hz, 2H), 4.76 (s, 2H), 2.37 (s, 3H), 1.26 (s, 9H).

255		474.34	99.23% Rt = 5.63 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.02 (bs, 1H), 8.65 (s, 1H), 7.89-7.87 (m, 2H), 7.84- 7.82 (m, 1H), 7.68-7.66 (m, 1H), 7.62-7.60 (m, 2H), 3.94 (s, 3H), 1.27 (s, 9H).

256		509.34	95.40% Rt = 6.17 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.20 (bs, 1H), 8.75 (s, 1H), 7.99 (s, 1H), 7.93-7.88 (m, 3H), 7.72-7.70 (d, J = 8.9 Hz, 1H), 7.64-7.62 (d, J = 8.5 Hz, 2H), 2.43 (s, 3H), 1.27 (s, 9H).

257		528.38	97.26% Rt = 6.46 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.04 (bs, 1H), 8.42 (s, 1H), 8.16 (s, 1H), 7.93-7.91 (d, J = 8.5, 2H), 7.85-7.83 (d, J = 8.9 Hz 1H), 7.69-7.67 (d, J = 8.9 Hz, 1H), 7.64-7.62 (d, J = 8.9 Hz, 2H), 3.98 (s, 3H), 2.43- 2.41 (q, J = 8.8 Hz, 2H), 1.27 (s, 9H), 0.97-0.93 (t, J = 7.2 Hz, 3H).

258		498.36	99.50% Rt = 6.39 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.95 (bs, 1H), 8.36-8.36 (d, J = 1.6 Hz, 2H), 7.92-7.90 (d, J = 8.6 Hz, 2H), 7.74-7.72 (d, J = 8.9 Hz, 1H), 7.62-7.60 (m, 3H), 7.54 (s, 1H), 4.69 (s, 2H), 2.26 (s, 3H), 1.26 (s, 9H).

259		514.38	98.04% Rt = 5.72 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.97 (bs, 1H), 8.23 (s, 1H), 7.91-7.89 (d, J = 7.8 Hz, 2H), 7.70 (s, 1H), 7.61-7.59 (d, J = 1.6 Hz, 3H), 7.35-7.34 (d, J = 6.6 Hz, 1H), 7.15-7.13 (d, J = 7.7 Hz, 1H), 4.73 (s, 2H), 2.24 (s, 3H), 1.27 (s, 9H).

260		514.38	97.80% Rt = 6.49 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.94 (bs, 1H), 9.92-7.90 (d, J = 8.4 Hz, 2H), 7.82-7.80 (d, J = 8.8 Hz, 1H), 7.68-7.62 (m, 3H), 7.56-7.54 (s, 1H), 7.32-7.29 (d, J = 8.6 Hz, 1H), 3.89 (s, 3H), 2.36 (s, 3H), 1.27 (s, 9H).

261		514.38	98.58% Rt = 6.65 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.04 (bs, 1H), 8.14 (s, 1H), 7.93-7.91 (d, J = 8.5 Hz, 2H), 7.84-7.26 (d, J = 8.8 Hz, 1H), 7.67-7.62 (m, 4H), 3.80 (s, 3H), 2.27 (s, 3H), 1.28 (s, 9H),

262		514.34	99.32% Rt = 5.55 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.94 (bs, 1H), 8.10 (s, 1H), 8.04 (s, 1H), 7.94-7.92 (d, J = 8.3 Hz, 2H), 7.15-1.13 (d, J = 9.0 Hz, 1H), 7.63-7.58 (m, 3H), 7.14 (s, 1H), 4.64 (s, 2H), 2.20 (s, 3H), 1.26 (s, 9H).

263		498.35	98.84% Rt = 6.31 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.99 (bs, 1H), 8.41 (s, 1H), 7.91-7.89 (d, J = 8.4 Hz, 2H), 7.84-7.82 (d, J = 8.9 Hz, 1H), 7.69-7.67 (d, J = 8.9 Hz, 1H), 7.64-7.62 (d, J = 8.5 Hz, 2H), 7.58 (s, 1H), 2.30 (s, 3H), 2.25 (s, 3H), 1.27 (s, 9H)

264		470.31	99.76% Rt = 6.01 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.03 (bs, 1H), 8.74- 8.73 (d, J = 4.8 Hz, 2H), 7.94- 7.92 (d, J = 8.4 Hz, 2H), 7.84- 7.82 (d, J = 9.2, 1H), 7.69-7.63 (m, 3H), 7.50-7.49 (d, J = 5.2, 2H), 1.27 (s, 9H).

265		530.35	98.09% Rt = 5.76 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.14 (bs, 1H), 7.94- 7.91 (d, J = 8.5 Hz, 2H), 7.88- 7.86 (d, J = 8.9 Hz, 1H), 7.69 (s, 1H), 7.67-7.61 (m, 2H), 7.58 (s, 1H), 7.26-7.23 (d, J = 9 Hz, 1H), 3.95 (s, 3H), 2.30 (s, 3H), 1.28 (s, 9H).

266		544.37	97.57% Rt = 5.71 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.07 (bs, 1H), 8.22 (s, 1H), 8.09 (s, 1H), 7.94-7.92 (q, J = 8.8 Hz, 2H), 7.84-7.82 (d, J = 9.2 Hz, 1H), 7.69-7.67 (d, J = 9.2 Hz, 1H), 7.64-7.62 (d, J = 8.4 Hz, 2H), 3.92 (s, 3H), 2.41-2.32 (m, 2H), 1.28 (s, 9H), 0.95-0.92 (m, 3H).

267		514.38	99.27% Rt = 5.49 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.04 (bs, 1H), 8.37 (s, 1H), 7.93-7.91 (d, J = 8.4 Hz, 2H), 7.84-7.82 (d, J = 8.8 Hz, 1H), 7.68-7.66 (d, J = 9.2 Hz, 1H), 7.64-7.62 (d, J = 8.8 Hz, 2H), 7.26 (s, 1H), 2.26 (s, 3H), 2.15 (s, 3H), 1.27 (s, 9H).

268		514.38	95.83% Rt = 6.47 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.96 (bs, 1H), 8.25 (s, 1H), 7.92-7.90 (d, J = 8.4 Hz, 2H), 7.83-7.81 (d, J = 8.8 Hz, 1H), 7.68-7.62 (m, 3H), 7.29 (s, 1H), 3.96 (s, 3H), 2.22 (s, 3H), 1.27 (s, 9H).

269		530.33	98.49% Rt = 5.67 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.15 (bs, 1H), 8.30 (s, 1H), 7.94-7.92 (d, J = 8.4 Hz, 2H), 7.89-7.86 (d, J = 9.2 Hz, 1H), 7.69-7.66 (d, J = 8.8 Hz, 1H), 7.65-7.63 (d, J = 8.4 Hz, 2H), 7.49 (s, 1H), 3.91 (s, 3H), 2.18 (s, 3H), 1.28 (s, 9H).

270		485.31	97.53% Rt = 6.04 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.08 (bs, 1H), 8.74 (d, J = 1.6 Hz, 1H), 8.61 (d, J = 1.6 Hz, 1H), 7.89-7.84 (m, 3H), 7.70-7.67 (d, J = 9.2 Hz, 1H), 7.62-7.60 (d, J = 8.4 Hz, 2H), 2.44 (s, 3H), 1.27 (s, 9H).

271		514.33	99.57% Rt = 6.15 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.09 (bs, 1H), 8.55- 8.54 (d, J = 5.6 Hz, 1H), 8.38 (s, 1H), 7.93-7.91 (d, J = 8.8 Hz, 2H), 7.85-7.83 (d, J = 8.8 Hz, 1H), 7.69-7.67 (d, J = 9.2 Hz, 1H), 7.64-7.62 (d, J = 8.8 Hz, 2H), 7.30-7.28 (d, J = 6.0 Hz, 1H), 4.19-4.14 (q, J = 6.8 Hz, 2H), 1.27 (s, 9H), 1.22- 1.18 (t, J = 6.8 Hz, 3H).

272		500.30	97.44% Rt = 5.33 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.04 (bs, 1H), 8.36 (s, 1H), 8.23-8.22 (d, J = 6.4 Hz, 1H), 7.93-7.91 (d, J = 8.4 Hz, 2H), 7.84-7.81 (d, J = 8.8 Hz, 1H), 7.69-7.67 (d, J = 8.8 Hz, 1H), 7.64-7.52 (d, J = 8.4 Hz, 2H), 7.41-7.40 (d, J = 6.8 Hz, 1H), 2.06 (s, 3H), 1.27 (s, 9H).

273		514.34	99.73% Rt = 6.60 min (1)	¹H NMR (400MHz, DMSO- d6): δ 9.93 (bs, 1H), 8.10-8.08 (d, J = 5.2 Hz, 1H), 7.92-7.90 (m, 2H), 7.76-7.74 (d, J = 8.8 Hz, 1H), 7.62-7.59 (m, 3H), 6.92-6.90 (d, J = 5.2 Hz, 1H), 6.74 (s, 1H), 4.68 (s, 2H), 3.81 (s, 3H), 1.26 (s, 9H).

274		486.08	99.08% Rt = 5.34 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.18 (bs, 1H), 8.49- 8.48 (d, J = 6 Hz, 1H), 7.93- 7.87 (m, 3H), 7.74-7.73 (d, J = 7.2 Hz, 1H), 7.69-7.61 (m, 4H), 7.54-7.50 (m, 1H), 1.28 (s, 9H).

275		485.32	99.59%, Rt = 6.08 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.06 (bs, 1H), 8.64 (s, 1H), 8.63 (s, 1H), 7.93-7.91 (d, J = 8.4 Hz, 2H), 7.86-7.84 (d, J = 9.2 Hz, 1H), 7.69-7.67 (d, J = 9.2 Hz, 1H), 7.64-7.62 (d, J = 8.4 Hz, 2H), 2.59 (s, 3H), 1.27 (s, 9H).

276		488.27	99.27%, Rt = 6.18 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.05 (bs, 1H), 8.71- 8.67 (m, 1H), 7.92-7.90 (d, J = 8.4 Hz, 2H), 7.86-7.84 (d, J = 8.8 Hz, 1H), 7.70-7.67 (d, J = 8.8 Hz, 1H), 7.64-7.62 (d, J = 8.4 Hz, 2H), 7.54-7.51 (m, 1H), 7.48-7.45 (m, 1H), 1.27 (s, 9H).

The following compounds were prepared essentially as in the methodology described in Example 10 using Compound 156 as the precursor to Compound 277, Compound 90 as the precursor to Compound 278, Compound 191 as the precursor to Compound 279 and Compound 65 as the precursor to Compound 280.


S.		LCMS	Purity
No.	Structure	(M + 1)	(LCMS)	1HNMR

277		466.10 (M − 1)	99.70% Rt = 5.32 (1)	1H NMR (400MHz, DMSO- d6): δ 10.28 (bs, 1H), 8.07-8.05 (d, J = 7.6 Hz, 1H), 8.01-7.99 (d, J = 8 Hz, 2H), 7.68-7.63 (m, 3H), 4.2 (m, 1H), 3.93 (M, 2H), 3.39 (m, 2 H), 2.27 (m, 2H), 1.64 (m, 2H), 1.26 (s, 9H).

278		491.08	96.92% Rt = 5.29 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.48 (bs, 1H), 8.40-8.39 (d, J = 2.4 Hz, 1H), 8.25 (s, 1H), 8.09 (s, 1H), 8.00-7.99 (d, J = 6.8 Hz, 2H), 7.75-7.73 (d, J = 8.4 Hz, 1H), 7.64-7.62 (d, J = 7.6 Hz, 2H),7.54-7.50 (d, J = 14.8 Hz, 1H), 3.86 (s, 3H), 1.28 (s, 9H).

279		475.12	92.53%, Rt = 5.30 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.49 (bs, 1H), 8.44 (m, 1H), 8.11-8.09 (d, J = 7.2 Hz, 1H), 8.03-8.00 (d, J = 8.4 Hz, 2H), 7.78 (m, 1H), 7.71-7.69 (d, J = 7.6 Hz, 1H), 7.65-7.63 (d, J = 8.4 Hz, 2H),7.46-7.44 (d, J = 7.6 Hz, 1H), 7.30-7.28 (m, 1H), 4.87 (s, 2 H), 1.28 (s, 9H).

280		491.08	98.67%, Rt = 4.77 min (1)	¹H NMR (400MHz, DMSO- d6): δ 10.49 (bs, 1H), 8.28 (s, 1H), 8.14-8.13 (d, J = 6 Hz, 1H), 7.99-7.97 (m, 3H), 7.67- 7.61 (m, 3H), 7.39-7.35 (m, 1H), 7.31-7.29 (d, J = 8 Hz, 1H), 4.72 (s, 2 H), 1.27 (s, 9H).

Example 29

Synthesis of Compound 281 [4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(2-oxo-1,2-dihydropyridin-3-yl)isoindolin-4-yl)benzenesulfonamide] and Compounds 282 to 285

Synthesis of XCVIII:

To a stirred solution of compound XXV (5 g, 0.022 mol) in acetic acid (15 ml) was added 2-methoxypyridin-3-amine (XCVII, 2.18 g, 0.017 mol) and the reaction mixture was heated at 120° C. for 48 h. The reaction mixture was cooled to room temperature and the acetic acid was removed under reduced pressure to obtain crude product, a mixture of XCVIII and XCIX. The crude material obtained was triturated with ethanol to provide the desired product, 4-chloro-7-nitro-2-(2-oxo-1,2-dihydropyridin-3-yl)isoindoline-1,3-dione, as a black solid (XCVIII; 2.3 g; 32% yield). ¹H NMR (400 MHz, DMSO-d6): δ 12.00 (bs, 1H), 8.36-8.34 (d, J=8.8 Hz, 1H), 8.21-8.19 (d, J=8.4 Hz, 1H), 7.68-7.65 (dd, J=2 Hz, 5.2 Hz, 1H), 7.60-7.59 (t, J=4.4 Hz, 1H), 6.39-7.35 (t, J=6.8 Hz, 1H). MS (M+1): 319.98

Synthesis of CC:

To a solution of compound XCVIII (4.1 g, 0.055 mol) in acetic acid (80 ml) under nitrogen atmosphere was added iron powder (3.5 g). The reaction mixture was stirred at room temperature for 12 h. This was filtered through a celite bed which was washed with ethyl acetate before the solvent was evaporated under reduced pressure to afford 4-amino-7-chloro-2-(2-oxo-1,2-dihydropyridin-3-yl)isoindoline-1,3-dione, as a yellow solid (C; 2.83 g; 76% yield). ¹H NMR (400 MHz, DMSO-d6): δ 12.17 (bs, 1H), 7.64-7.62 (d, J=6.8 Hz, 1H), 7.54-7.53 (d, J=4.4 Hz, 1H), 7.48-7.46 (d, J=8.8 Hz, 1H), 7.06-7.04 (d, J=9.2 Hz, 1H), 6.68 (bs, 2H), 6.34-6.31 (t, J=6.8 Hz, 1H). MS (M+1): 289.97

Synthesis of 281: 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(2-oxo-1,2-dihydropyridin-3-yl)isoindolin-4-yl)benzenesulfonamide

A solution of compound CC (2 g, 0.006 mol) in pyridine (40 ml) was stirred and cooled to 0° C. 4-(tert-butyl)benzenesulfonyl chloride (V; 6.41 g, 0.027 mmol) was added. The reaction mixture was stirred at 110° C. for 12 h and concentrated under reduced pressure before dilution with water. The resulting aqueous layer was extracted with ethyl acetate. The organic layer was washed successively with brine solution, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound CI. To this material was added 1M TBAF in THF solution (22 ml) and the resulting mixture was stirred for 3 h at room temperature. The reaction mixture was concentrated and diluted with ethyl acetate. The organic layer was washed with water, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound, which was purified by column chromatography using 45% ethyl acetate in hexane to afford the title compound, 4-(tert-butyl)-N-(7-chloro-1,3-dioxo-2-(2-oxo-1,2-dihydropyridin-3-yl)isoindolin-4-yl)benzenesulfonamide, as an off-white solid (281; 0.34 g, 16% yield). ¹H NMR (400 MHz, DMSO-d6): ¹H NMR (400 MHz, DMSO-d6): δ 12.25 (bs, 1H), 10.02 (bs, 1H), 7.93-7.91 (d, J=8.4 Hz, 2H), 7.83-7.81 (d, J=8 Hz, 1H), 7.66-7.57 (m, 5H), 6.36-6.33 (t, J=5.6 Hz, 1H), 1.27 (s, 9H). MS (M+1): 486.19. (LCMS purity 98.45%, RT=5.50 min) (1).
The following compounds were prepared in a similar manner as mentioned in above scheme using the appropriate amines in the first step:


		LCMS
S. No.	Structure	(M + 1)	Purity (LCMS)	1HNMR

282		500.33	99.50% Rt = 5.59 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 9.95 (bs, 1H), 7.94-7.92 (d, J = 8.8 Hz, 2H), 7.88-7.87 (d, J = 2.4 Hz, 1H), 7.82-7.80 (d, J = 8 Hz, 1H), 7.67-7.63 (m, 3H), 7.45-7.42 (m, 1H), 6.50-6.48 (d, J = 5.6 Hz, 1H), 3.46 (s, 3H), 1.27 (s, 9H).

283		528.31	97.31% Rt = 6.08 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 11.35 (bs, 1H), 9.93 (bs, 1H), 7.92-7.90 (d, J = 8.4 Hz, 2H), 7.71-7.69 (d, J = 8.8 Hz, 1H), 7.62-7.60 (d, J = 8.4 Hz, 2H), 7.56-7.53 (d, J = 8.8 Hz, 1H), 5.84 (s, 1H), 4.51 (s, 2H), 2.19 (s, 3H), 2.08 (s, 3H), 1.27 (s, 9H).

284		500.31	97.59% Rt = 5.71 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 11.74 (bs, 1H), 9.94 (bs, 1H), 7.93-7.91 (d, J = 8 Hz, 2H), 7.75-7.73 (d, J = 8.8 Hz, 1H), 7.63-7.59 (m, 3H), 7.32- 7.27 (m, 2H), 6.12-6.09 (m, 1H), 4.44 (s, 2H), 1.26 (s, 9H).

285		514.34	96.76% Rt = 5.83 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 9.93 (bs, 1H), 7.93-7.91 (d, J = 8.24 Hz, 2H), 7.81-7.79 (d, J = 8.64 Hz, 1H), 7.73 (s, 1H), 7.67-7.63 (m, 3H), 7.32 (s, 1H), 3.47 (s, 3H), 2.02 (s, 3H), 1.27 (s, 9H).

Example 30

Synthesis of Compound 286 [ethyl 3-(4-((4-(tert-butyl)phenyl)sulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)picolinate]; Compound 287 [3-(4-((4-(tert-butyl)phenyl)sulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)picolinic acid] and Compounds 288 to 292

Synthesis of CIII:

To a stirred solution of compound XXV (5 g, 0.02 mol) in acetic acid (15 ml) was added ethyl 3-aminopicolinate (CII, 1.69 g, 0.01 mol) and the reaction mixture was heated at 120° C. for 12 h. The reaction mixture was cooled to room temperature and the acetic acid removed under reduced pressure. This was followed by washing with hexane to leave crude product ethyl 3-(4-chloro-7-nitro-1,3-dioxoisoindolin-2-yl)picolinate as an off white solid (CIII) pure enough for use in the next step. ¹H NMR (400 MHz, DMSO-d6): δ 8.88-8.86 (d, J=4.4 Hz, 1H), 8.10-8.08 (d, J=8.4 Hz, 1H), 7.91-7.89 (d, J=8.8 Hz, 1H), 7.82-7.80 (d, J=8 Hz, 1H), 7.69-7.66 (m, 1H), 4.40-4.35 (q, J=7.2 Hz, 6.8 Hz, 2H), 1.37-1.31 (t, J=6.8 Hz, 3H). MS (M+1): 376.03

Synthesis of CIV:

To a solution of crude compound CIII (2.1 g, 0.055 mol) in acetic acid (30 ml) under a nitrogen atmosphere was added iron powder (2 g). The reaction mixture was stirred at room temperature for 5 h. The reaction mass was filtered through a celite bed which was washed with ethyl acetate and the solvent was evaporated under reduced pressure to afford ethyl 3-(4-amino-7-chloro-1,3-dioxoisoindolin-2-yl)picolinate as a yellow solid (CIV; 1.8 g; 93% yield). ¹H NMR (400 MHz, DMSO-d6): δ 8.78-8.76 (m, 1H), 8.07-8.05 (m, 1H), 7.88-7.82 (m, 1H), 7.53-7.50 (d, J=8.8 Hz, 1H), 7.10-7.08 (d, J=8.8 Hz, 1H), 6.78 (bs, 2H), 4.20-4.15 (q, J=7.2 Hz, 6.8 Hz, 2H), 1.10-1.06 (t, J=7.2 Hz, 3H). MS (M+1): 346.03

Synthesis of 286: ethyl 3-(4-((4-(tert-butyl)phenyl)sulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)picolinate

A stirred solution of CIV (1.8 g, 0.005 mol) in pyridine (20 ml) was cooled to 0° C. and 4-(tert-butyl) benzenesulfonyl chloride (V, 3.63 g, 0.015 mmol) was added. The reaction mixture was stirred at 90° C. for 8 h and was then cooled, concentrated and diluted with water. The reaction mixture was extracted with ethyl acetate. The organic layer was washed with brine solution, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound CV. To this was added 1M TBAF in THF solution (15 ml) and the resulting solution was stirred for 5 h at room temperature. The reaction mixture was concentrated and diluted with ethyl acetate. The organic layer was washed with water, dried over anhydrous Na₂SO₄, filtered and evaporated under reduced pressure to obtain the crude compound which was purified by column chromatography using 20% ethyl acetate in hexane to afford the title compound, ethyl 3-(4-((4-(tert-butyl) phenyl) sulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)picolinate as an off white solid (286; 1.2 g, 42.8% yield). ¹H NMR (400 MHz, DMSO-d6): δ 10.16 (bs, 1H), 8.82-8.81 (d, J=3.6 Hz, 1H), 8.04-8.03 (d, J=7.6 Hz, 1H), 7.91-7.85 (m, 4H), 7.72-7.70 (d, J=9.2 Hz, 1H), 7.63-7.61 (d, J=8.4 Hz, 2H), 4.16 (q, J=7.2 Hz, 6.8 Hz, 2H), 1.27 (s, 9H), 0.99-0.95 (t, J=7.2 Hz, 3H). MS (M+1): 542.10. (LCMS purity 96.98%, RT=6.11 min) (1).

Synthesis of 287; 3-(4-((4-(tert-butyl)phenyl)sulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)picolinic acid

To a stirred solution of (286; 0.2 g, 0.37 mmol) in methanol (5 ml), was added a sodium hydroxide (0.04 g, 1.1 mmol) solution in water. The reaction was stirred at room temperature for 0.5 h whereupon the solvent was concentrated under reduced pressure and the residue diluted with water followed by acidification with aqueous citric acid till pH 5. The aqueous layer was extracted with ethyl acetate. The organic layer was washed with brine, dried over Na₂SO₄, filtered and concentrated under reduced pressure to obtained the crude compound as a mixture of CVI and 287. To a stirred solution of this mixture in toluene (3 ml) was added a catalytic quantity of para-toluene sulfonic acid and the reaction mixture heated at 90° C. for 1 h. The reaction mixture was concentrated and then diluted with ethyl acetate. The organic layer was washed with water, dried over anhydrous Na₂SO₄, filtered and the organic solvent was evaporated under reduced pressure to obtain the crude compound which was purified by preparative HPLC to afford the title compound, 3-(4-((4-(tert-butyl)phenyl)sulfonamido)-7-chloro-1,3-dioxoisoindolin-2-yl)picolinic acid as a white solid (287; 0.05 g, 26.5% yield). ¹H NMR (400 MHz, DMSO-d6): δ 13.42 (bs, 1H), 10.18 (bs, 1H), 8.80 (m, 1H), 8.02-8.00 (d, J=8 Hz, 1H), 7.95-7.93 (d, J=8.4 Hz, 2H), 7.87-7.82 (m, 2H), 7.67-7.63 (m, 3H), 1.27 (s, 9H). MS (M+1): 514.30 (LCMS purity 98.28%).
The following compounds were prepared in a similar manner as mentioned in above reaction using appropriate amines instead of CII at step-1 with the final step described only used where necessary to generate an acid functionality:


		LCMS
S. No.	Structure	(M + 1)	Purity (LCMS)	1HNMR

288		516.98 (M − 1)	99.30% Rt = 5.22 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 13.39 (bs, 1H), 10.08 (bs, 1H), 8.01-7.99 (d, J = 5.6 Hz, 1H), 7.94-7.91 (d, J = 8.8 Hz, 2H), 7.85-7.83 (d, J = 8.8 Hz, 1H), 7.67-7.62 (m, 3H), 7.20-7.19 (d, J = 4.8 Hz, 1H), 1.27 (s, 9H).

289		517.00	97.90% Rt = 4.84 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 12.88 (bs, 1H), 10.08 (bs, 1H), 8.03 (s, 1H), 7.94- 7.92 (d, J = 8.8 Hz, 2H), 7.83- 7.81 (d, J = 8.8 Hz, 1H), 7.65- 7.62 (m, 3H), 3.98 (s, 3H), 1.28 (s, 9H).

290		558.05	98.01% Rt = 5.47 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.24 (bs, 1H), 8.53-8.51 (d, J = 6.4 Hz, 1H), 7.93-7.91 (d, J = 8 Hz, 2H), 7.88-7.85 (d, J = 8.4 Hz, 1H), 7.76-7.72 (m, 1H), 7.65-7.59 (m, 4H), 4.18- 4.17 (q, J = 6.8 Hz, 7.2 Hz, 2H), 1.28 (s, 9H), 0.97-0.95 (t, J = 6.8 Hz, 3H).

291		531.05	98.26% Rt = 5.85 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 10.09 (bs, 1H), 7.95-7.93 (d, J = 8.4 Hz, 2H), 7.86-7.84 (d, J = 8.8 Hz, 1H), 7.70-7.67 (d, J = 8.8 Hz, 1H), 7.65-7.63 (d, J = 8.4 Hz, 2H), 6.83 (s, 1H), 3.82 (s, 3H), 3.80 (s, 3H), 1.28 (s, 9H).

292		531.07	99.00% Rt = 6.33 min (1)	¹H NMR (400 MHz, DMSO- d6): δ 9.97 (bs, 1H), 7.91-7.88 (d, J = 8.4 Hz, 2H), 7.84-7.81 (d, J = 9.2 Hz, 1H), 7.68-7.62 (m, 3H), 6.90 (s, 1H), 4.13 (s, 3H), 3.86 (s, 3H), 1.27 (s, 9H).

Example 31

Biological Activity: FLIPR Assay Using hCCR9 Over Expressed Cells
A calcium flux assay was used to determine the ability of the compounds to interfere with the binding between CCR9 and its chemokine ligand (TECK) in Chem1-hCCR9 overexpressing cells. hCCR9 overexpressing cells were seeded (25,000 cells/well) into black Poly-D-Lysine coated clear bottom 96-well plates (BD Biosciences, Cat #356640) and incubated overnight at 37° C./5% CO₂in a humidified incubator. Media was aspirated and cells washed twice with 100 μL assay buffer (lx HBSS, 20 mM HEPES) containing 2.5 mM Probenecid. A 0.3× Fluo-4 NW calcium dye was prepared in assay buffer containing 5 mM Probenecid and stored in the dark. Each well was loaded with 100 μL of 0.3× Fluo-4 NW calcium dye and incubated at 37° C./5% CO₂for 60 minutes and then at room temperature for 30 minutes. A half-log serially diluted concentration response curve was prepared at a 3× final assay concentration for each compound (10 μM-0.1 nM final assay concentration) and 50 μL of the compound then transferred to the cells (150 μL final volume) for 60 minutes prior to stimulation (30 minutes at 37° C./5% CO₂and 30 minutes at room temperature). TECK was diluted to 4× its EC₈₀in assay buffer (containing 0.1% [w/v] bovine serum albumin [BSA]) and 50 μL dispensed through the fluorometric imaging plate reader (FLIPR) instrument to stimulate the cells (200 μL final volume). The increase in intracellular calcium levels was measured with the FLIPR instrument. The potency of the compound as a CCR9 antagonist was calculated as an IC₅₀using GraphPad Prism software (variable slope four parameter). The Ki of the compound was determined from the IC₅₀values using the following equation.
Ki calculation: IC₅₀/1+(Agonist (TECK) conc. used in assay/EC₅₀of agonist (TECK) generated on day of experiment)


Compound number	Structure	Ki (nM)

1		1244

6		5632

43		42

44		179

45		314

47		309

64		27

65		74

66		269

82		98

90		174

103		242

104		239

119		35

120		90

121		337

126		343

128		447

133		158

138		138

144		23

145		73

148		94

149		397

153		43

155		135

160		776

175		12

176		13

179		57

182		58

183		3

184		24

185		23

188		89

189		34

190		81

191		33

192		12

193		21

194		6

195		42

196		103

197		40

199		10

200		92

201		17

202		49

203		59

204		30

207		36

208		16

209		77

211		61

212		60

213		23

214		53

216		49

217		39

218		26

219		30

220		42

222		12

224		6

225		51

226		23

227		32

228		6

229		63

230		6

231		89

232		3

233		21

234		43

235		94

236		32

237		9

238		13

239		23

241		7

242		56

243		115

244		3

245		54

247		51

249		60

250		56

251		148

254		68

255		72

256		18

257		21

258		67

259		45

260		22

261		11

262		119

263		25

264		18

266		25

267		59

270		61

271		35

272		29

274		50

275		54

276		91

278		17

279		17

280		84

281		4

282		23

283		20

284		12

285		78

286		14

287		10

288		23

289		109

290		14

291		15

292		16

Example 32

Biological Activity: FLIPR Assay Using MOLT4 Cells

A calcium flux assay was used to determine the ability of the compounds to interfere with the binding between CCR9 and its chemokine ligand (TECK) in MOLT4 cells (a human T-cell line). MOLT4 cells were seeded (100,000 cells/well) in corning cell culture plates (Cat #3603) in assay buffer (1×HBSS, 20 mM HEPES) containing 2.5 mM Probenecid. The plate was centrifuged at 1200 rpm for 3 minutes and incubated at 37° C./5% CO₂for 2 hours. A 0.3× Fluo-4 NW calcium dye was prepared in assay buffer containing 5 mM Probenecid and stored in the dark. Each well was loaded with 25 μL of 0.3× Fluo-4 NW calcium dye and incubated at 37° C./5% CO₂for 60 minutes and then at room temperature for 30 minutes. A half-log serially diluted concentration response curve was prepared at a 4× concentration for each (10 μM-0.1 nM final assay concentration) and 25 μL of the compound then transferred to the cells (100 μL final volume) for 60 minutes prior to stimulation (30 minutes at 37° C./5% CO₂and 30 minutes at room temperature). TECK was diluted to 5× its EC₅₀in assay buffer (containing 0.1% [w/v] bovine serum albumin [BSA]) and 25 μL dispensed through the FLIPR instrument to stimulate the cells (125 μL final volume). The increased in intracellular calcium levels was measured with the FLIPR instrument. The potency of the compound as CCR9 antagonist was calculated as an IC₅₀using GraphPad Prism software (variable slope four parameter). The Ki of the compound was determined from the IC₅₀values using the following equation.
Ki calculation:IC₅₀/1+(Agonist(TECK)conc. used in assay/EC₅₀of agonist(TECK)generated on day of experiment)


Compound number	Structure	Ki (nM)

1		479

4		2713

5		123

7		206

8		1322

12		618

14		1555

19		758

36		1210

39		611

40		1355

41		167

43		25

44		16

45		52

47		34

48		171

52		187

57		38

64		22

65		13

66		115

79		33

80		173

81		148

82		19

90		22

91		50

92		255

93		217

94		244

103		128

104		30

105		311

107		318

116		100

117		184

118		78

119		2

120		74

121		192

122		175

124		224

125		243

126		158

127		153

128		244

132		252

133		220

138		83

140		216

142		41

143		64

144		3

145		129

146		154

148		36

149		53

151		42

152		245

153		7

154		32

155		44

157		189

160		456

161		568

162		1000

175		3

176		6

177		73

178		164

179		16

180		78

182		19

183		3

184		16

185		7

187		33

188		15

189		8

190		24

191		20

192		4

193		16

194		4

195		31

196		46

197		21

198		76

199		6

200		45

201		8

202		22

203		38

204		7

205		46

207		30

208		24

209		30

210		44

211		33

212		59

213		12

214		20

215		85

216		23

217		35

218		7

219		10

220		12

221		212

222		6

224		5

225		54

226		21

227		14

228		5

229		46

230		8

231		50

232		7

233		19

234		45

235		123

236		51

237		28

238		14

239		13

240		84

241		5

242		18

243		34

244		6

245		47

246		125

247		78

248		70

249		33

250		23

251		51

252		33

253		41

254		58

255		17

256		18

257		25

258		23

259		13

260		16

261		16

262		46

263		11

264		11

265		41

266		10

267		27

268		54

269		76

270		23

271		9

272		9

273		108

274		34

275		32

276		22

277		28

278		10

279		18

280		37

281		5

282		9

283		15

284		7

285		16

286		10

287		22

288		3

289		104

290		7

291		5

292		11

Claims

1-35. (canceled)

36. A method of treating, preventing, or ameliorating a disease or condition associated with CCR9 activation in a subject, the method comprising administering an effective amount of a compound of Formula (I), or a salt, solvate, or solvate of a salt thereof:

in which:

R₁is selected from hydrogen, methyl, and ethyl;

X is selected from a direct bond and (CR₅R₆)_p;

p is 1, 2, 3, 4, or 5;

each R₅is independently selected from hydrogen, methyl, and fluoro;

each R₆is independently selected from hydrogen, methyl, and fluoro;

R₂is selected from optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted C_3-7heterocycloalkyl;

each R₃is independently selected from halo, cyano, C_1-6alkyl, methanesulfonyl, C_1-6alkoxy, haloalkyl, haloalkoxy, and C_3-7cycloalkyl;

n is 0, 1, or 2;

each R₄is Z_q1B;

m is 0, 1, 2, or 3;

q₁is 0, 1, 2, 3, 4, 5, or 6;

each Z is independently selected from CR₇R₈, O, C═O, SO₂, and NR₉;

each R₇is independently selected from hydrogen, methyl, ethyl, and halo;

each R₈is independently selected from hydrogen, methyl, ethyl, and halo;

each R₉is independently selected from hydrogen, methyl, and ethyl;

each B is independently selected from hydrogen, halo, CN, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted cycloalkyl, and A;

A is

Q is selected from CH₂, O, NH, and NCH₃;

x is 0, 1, 2, 3, or 4, and y is 1, 2, 3, 4, or 5, the total of x and y being greater or equal to 1 and less than or equal to 5 (1≤x+y≤5).

37. The method of claim 36, wherein R₁is hydrogen.

38. The method of claim 36, wherein X is a direct bond.

39. The method of claim 36, wherein X is CH₂.

40. The method of claim 36, wherein R₂is selected from optionally substituted aryl and optionally substituted heteroaryl.

41. The method of claim 40, wherein R₂is selected from optionally substituted phenyl, optionally substituted pyridyl, optionally substituted thiophenyl, optionally substituted pyrazolyl, optionally substituted pyrimidinyl, optionally substituted imidazolyl, and optionally substituted thiazolyl.

42. The method of claim 40, wherein R₂is selected from cyanophenyl, acetylphenyl, methoxy-phenyl, pyridine N-oxide, methyl-pyridine N-oxide, methoxy-pyridine N-oxide, ethoxy-pyridine N-oxide, pyridyl, methoxy-pyridyl, ethoxy-pyridyl, methyl-pyridyl, cyano-pyridyl, thiophenyl, carboxy-thiophenyl, carboxymethyl-thiophenyl, pyrazolyl, methyl-pyrazolyl, imidazolyl, and methyl-imidazolyl.

43. The method of claim 36, wherein each R₃is independently selected from halo, cyano, C_1-3alkyl, C_1-3alkoxy, C_1-3haloalkyl, and cyclopropyl.

44. The method of claim 43, wherein each R₃is independently selected from chloro, cyano, methyl, methoxy, propoxy, isopropoxy, trifluoromethyl, and cyclopropyl.

45. The method of claim 36, wherein n is 0 or 1.

46. The method of claim 36, wherein R₄is Z_q1B; q₁is 0; and each B is independently selected from halo, CN, optionally substituted aryl, optionally substituted heteroaryl, and A.

47. The method of claim 36, wherein R₄is Z_q1B; q₁is 1, 2, or 3; each Z is independently C_1-3alkyl; and each B is independently selected from halo, CN, optionally substituted aryl, optionally substituted heteroaryl, and A.

48. The method of claim 36, wherein R₄is Z_q1B; q₁is 1, 2, 3, 4, 5, or 6; each Z is independently selected from CR₇R₈, O, C═O, and SO₂; each R₇is independently selected from hydrogen, methyl, and halo; each R₈is independently selected from hydrogen, methyl, and halo;

and B is selected from hydrogen, halo, and cyano.

49. The method of claim 48, wherein each R₄is independently selected from butyl, tert-butyl, propyl, iso-propyl, methyl, COCH₃, C(CH₃)(CH₃)CN, trifluoromethyl, trifluoromethoxy, difluoromethoxy, and methoxy.

50. The method of claim 36, wherein m is 1 or 2.

51. The method of claim 50, wherein m is 1 and R₄is para to the sulphonamide

52. The method of claim 50, wherein m is 2 and one R₄group is meta to the sulphonamide, and the other R₄group is para to the sulfonamide.

53. The method of claim 50, wherein R₁is hydrogen; X is CH₂; R₂is an optionally substituted heteroaryl; n is 0; m is 1; and R₄is trifluoromethoxy.

54. The method of claim 50, wherein R₁is hydrogen; X is a direct bond; R₂is selected from optionally substituted aryl and optionally substituted heteroaryl; n is 0; m is 2; one R₄group is halo; and the other R₄group is trifluoromethyl.

55. The method of claim 50, wherein R₁is hydrogen; X is a direct bond; R₂is selected from optionally substituted aryl and optionally substituted heteroaryl; n is 0; m is 1; and R₄is selected from butyl, tert-butyl, trifluoromethyl, trifluoromethoxy, and difluoromethoxy.

56. The method of claim 36, wherein R₁is hydrogen; X is a direct bond or CH₂; R₂is selected from optionally substituted aryl and optionally substituted heteroaryl; n is 1; R₃is halo or cyano; m is 1; and R₄is butyl or tert-butyl.

57. The method of claim 56, wherein R₂is selected from optionally substituted pyridyl, optionally substituted phenyl, optionally substituted pyrazolyl, optionally substituted imidazolyl, and optionally substituted thiophenyl, wherein optional substituents are selected from O⁻, OCH₃, OC₂H₅, CH₃, carboxy, carboxymethyl, and CN; R₃is chloro; and R₄is tert-butyl.

58. The method of claim 36, wherein the compound is

or a salt, solvate, or solvate of a salt thereof.

59. The method of claim 36, wherein the disease or condition is an inflammatory disease or condition, an immune disorder, or cancer.

60. The method of claim 36, wherein the disease or condition is an inflammatory bowel disease, an allergic disease, an autoimmune disease, an inflammatory dermatosis, a spondyloarthropathy, a graft rejection, a graft versus host disease, atherosclerosis, a neurodegenerative disease, a liver disease, or cancer.

61. The method of claim 36, wherein the disease or condition is an inflammatory bowel disease.

62. The method of claim 61, wherein the inflammatory bowel disease is collagenous colitis, lymphocytic colitis, ischaemic colitis, diversion colitis, Behcet's disease, indeterminate colitis, ileitis, enteritis, Crohn's disease, or ulcerative colitis.

63. The method of claim 61, wherein the inflammatory bowel disease is Crohn's disease or ulcerative colitis.

64. A process for the preparation of a compound of Formula (I) of claim 36, the process comprising reacting an anhydride (A) with a primary amine (B) to produce a phthalimide (C), reducing the nitro group in the phthalimide (C) to form an aminophthalimide (D), then:

(i) converting the aminophthalimide (D) to a secondary sulfonamide (F) using a sulfonyl chloride (E), and optionally derivatizing the secondary sulfonamide (F) to a tertiary sulfonamide (H); or

(ii) converting the aminophthalimide (D) to a secondary amine (G), and converting the secondary amine (G) to a tertiary sulfonamide (H) using a sulfonyl chloride (E); and

(iii) optionally adding appropriate substituents to an R₂, R₃, or R₄group of the secondary sulfonamide (F) or of the tertiary sulfonamide (H);

wherein Z is a halogen; R₁, X, R₂, R₃, n, R₄, and m have the meanings given for the compound of Formula (I) in claim 1, and R₁, R₂, R₃, R₄in intermediate compounds may represent protected forms of these groups.