US20170020132A1 - Fertilizing and bio stimulating product containing fructo oligosaccharides, application method and uses - Google Patents

Fertilizing and bio stimulating product containing fructo oligosaccharides, application method and uses Download PDF

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Publication number
US20170020132A1
US20170020132A1 US15/302,410 US201515302410A US2017020132A1 US 20170020132 A1 US20170020132 A1 US 20170020132A1 US 201515302410 A US201515302410 A US 201515302410A US 2017020132 A1 US2017020132 A1 US 2017020132A1
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fos
fructooligosaccharide
plants
composition
fertilizing
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Inventor
Javier ERRO GARCES
Oscar URRATIA SAGARDIA
Maria GARNICA OCHOA
Diane Lemenager
Esther CASANOVA PORTILLO
Jose Maria Garcia-Mina Freire
Jean-Claude Yvin
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Agro Innovation International SAS
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Agro Innovation International SAS
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Assigned to AGRO INNOVATION INTERNATIONAL reassignment AGRO INNOVATION INTERNATIONAL ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: CASANOVA PORTILLO, ESTHER, YVIN, JEAN-CLAUDE, LEMENAGER, DIANE, ERRO GARCES, Javier, GARCIA-MINA FREIRE, JOSE MARIA, GARNICA OCHOA, Maria, URRUTIA SAGARDIA, Oscar
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
    • C05B17/02—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal containing manganese
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity

Definitions

  • the present invention relates to the use of fructooligosaccharides to elicit mechanisms for absorption of inorganic nutrients and as agents for stimulating plant defenses against pathogens.
  • the invention also relates to fertilizing and biostimulating products containing such fructooligosaccharides, and also to a method for spreading these fructooligosaccharides on crops.
  • One of the strategies for increasing yields consists in producing more effective fertilizers, for example by reducing the loss of nutritive elements into the soil.
  • urease and nitrification inhibitors have been used with the purpose of reducing the volatilization of ammonia and the leaching of nitrates.
  • Another supplementary strategy consists in developing molecules which increase the plant's capacity to absorb nutrients from the soil, or in stimulating the plant's natural defenses against pathogens.
  • a fructooligosaccharide comprising thirteen fructose units has been described as a growth activator for plants at different stages of development (CN 2007/101006777 et J. Phytopathol., 157, pp 201-207, 2009). This effect is attributed to the activation of the production of salicylic acid and the glycosylated homolog thereof in the leaves of treated plants.
  • the fructooligosaccharide is obtained by aqueous-phase extraction from burdock ( Arctium lappa ) roots followed by enzymatic deprotonation, precipitation in ethanol and purification by chromatography.
  • the invention relates to fructooligosaccharides of lower molecular weight than that of the fructooligosaccharide described in the prior art. Indeed, the inventors have discovered that these fructooligosaccharides cause a better rate of nutrient absorption by the roots of the plants, and further stimulate the plant's natural defenses. This effect relates to all inorganic nutrients, in particular nitrogen, phosphorus and calcium. Indeed, the fructooligosaccharides used within the context of the invention have better water-solubility than the fructooligosaccharides of the prior art. The inventors have found that inulin cannot be applied in the form of a fertilizer in aqueous solution, since this compound cannot remain stable in the stock solution before application thereof to the crops.
  • the product of the invention enables increased production of organic nitrogen in the plant, responding to the growth requirements of the crop, which is manifested in an increased yield.
  • the product of the invention also enables an increase in the protein content and the nutritional value of protein-producers and fodder.
  • the product of the invention also makes it possible to reduce the risks of toxicity caused by an excessive accumulation of ammonium ions in the leaves or to reduce the accumulation of nitrates in the leaves.
  • a subject of the present invention is a fertilizing and/or biostimulating composition containing fructooligosaccharides and also a process for treating plants or soils using them.
  • fertilizerizing composition is intended to mean any product, the use of which is intended to ensure, or improve, plant nutrition.
  • “Fructooligosaccharide” is intended to mean a compound comprising several fructose units and optionally another monosaccharide unit.
  • biostimulating composition is intended to mean any product, the use of which is intended to ensure the ability of the plants to stimulate their leaf or root metabolism.
  • a subject of the invention is a fertilizing and/or biostimulating composition containing at least one fructooligosaccharide (FOS) comprising from 2 to 10 monosaccharide units, which fructooligosaccharide is optionally sulfated.
  • the composition may contain the fructooligosaccharide in combination with one or more fertilizing materials.
  • the fructooligosaccharide is preferably in an effective amount in the composition in order to cause the desired effect.
  • the amount of FOS supplied to the plants must be sufficient to stimulate the eliciting of mechanisms for absorbing inorganic nutrients. This amount is variable depending on the nature of the plant to be treated and the mode of treatment (via the leaves or via the roots). This amount may especially be determined on a case by case basis by employing macroarray tests as defined below.
  • the fructooligosaccharides used within the context of the present invention are polymers comprising at least one fructose unit and at least one C 3 -C 6 monosaccharide unit, preferably a C 6 monosaccharide unit other than fructose, for example chosen from glucose, mannose and xylose.
  • the fructooligosaccharide (FOS) used within the context of the present invention comprises from 2 to 10 monosaccharide units (2 and 10 being included), said monosaccharide units comprising at least one fructose. It is preferred that the fructooligosaccharide (FOS) used within the context of the present invention comprise from 2 to 9 monosaccharide units (2 and 9 being included), or from 2 to 8 monosaccharide units (2 and 8 being included), or else from 2 to 7 monosaccharide units (2 and 7 being included), or else from 2 to 6 monosaccharide units (2 and 6 being included), for example from 2 to 6 monosaccharide units (2 and 6 being included), from 2 to 5 monosaccharide units (2 and 5 being included), from 2 to 4 monosaccharide units (2 and 4 being included).
  • the fructooligosaccharide (FOS) used within the context of the present invention may be a mixture of the above fructooligosaccharides.
  • the fructooligosaccharide (FOS) used within the context of the present invention advantageously corresponds:
  • the fructooligosaccharide (FOS) used within the context of the present invention may be a mixture of Glucose-(Fructose) n , or Mannose-(Fructose) n , compounds such that n ranges from 1 to 3 (1 and 3 being included).
  • the FOSs may be obtained from the abovementioned species by a method comprising the steps of washing, grinding, extraction (solid-liquid separation) and optionally fractionation and concentration.
  • the FOSs may also be obtained by acid or enzymatic hydrolysis, from the abovementioned extracts.
  • the fructooligosaccharides are obtained by hydrolysis of inulin or by hydrolysis of oligosaccharides obtained from inulin.
  • the hydrolysis may be carried out enzymatically, chemically or microbially (for example by Bifidobacterium ).
  • the fructooligosaccharides are obtained by enzymatic hydrolysis of inulin.
  • Inulin is a polysaccharide consisting of D-fructose units bonded by ⁇ (2,1) linkages and of a D-glucose end group bonded by an ⁇ (1,2) linkage.
  • the degree of polymerization of inulin is generally of the order of 40 to 60 monosaccharide units.
  • Inulin may be extracted from the roots of various plants, in particular Jerusalem artichoke and chicory.
  • the plant extract containing FOSs obtained according to one of the methods described above may be more or less concentrated depending on the intended use. Total dehydration of the extract enables it to be made available in water-soluble pulverulent form. The dehydration may be obtained by drum drying or spray drying.
  • the fructooligosaccharides may be obtained by chemical synthesis.
  • the fructooligosaccharides may be obtained by transfructosylation of sucrose, for example by the transfructosylation action of an Aspergillus niger ⁇ -fructosidase on sucrose.
  • the fructooligosaccharides may be sulfated, for example by reaction of an FOS as described above with sulfuric acid in the presence of pyridine.
  • the FOSs described above may be used alone or in a mixture with inorganic fertilizers, to prepare the fertilizing and/or biostimulating product of the invention.
  • fertilizers are defined as fertilizing materials, the main function of which is to supply plants with elements directly useful to their nutrition (major fertilizing elements, secondary fertilizing elements and trace elements).
  • root or leaf fertilizers generally use sources of nitrogen, phosphorus and potassium as well as trace elements and amino acids.
  • the nitrogen is generally supplied in the form of nitrate or ammonium, the use of which in large amounts poses problems from an ecological viewpoint.
  • the fertilizing and/or biostimulating product of the invention containing FOSs may therefore be used as a supplement in conventional fertilizing compositions such as fertilizers in order to activate inorganic nitrogen absorption and the assimilation thereof in the form of proteins.
  • the fertilizing and/or biostimulating product of the invention may therefore contain one or more fertilizing materials chosen from urea, ammonium sulfate, ammonium nitrate, natural phosphate, potassium chloride, ammonium sulfate, magnesium nitrate, manganese nitrate, zinc nitrate, copper nitrate, phosphoric acid and boric add.
  • fertilizing materials chosen from urea, ammonium sulfate, ammonium nitrate, natural phosphate, potassium chloride, ammonium sulfate, magnesium nitrate, manganese nitrate, zinc nitrate, copper nitrate, phosphoric acid and boric add.
  • the product of the invention may contain biostimulating agents chosen from humic acids, extracts of marine algae, amino acids, plant hormones or a mixture thereof.
  • the fructooligosaccharide represents for example from 0.1 to 10% by weight, for example 1% by weight, of the weight of the composition.
  • the product of the invention When it is formulated in liquid form, the product of the invention contains for example from 0.1 to 5% by weight of FOS. When it is formulated in solid form, the product of the invention contains for example from 1 to 10% by weight of FOS.
  • the product of the invention may be in the form of a product chosen from calcareous enriching agents, organic enriching agents and crop supports, root fertilizers of NP, PK, NPK type, leaf fertilizers or else nutritive solutions for roots.
  • Another subject of the invention is a method for fertilizing plants to improve their absorption of inorganic nutrients, which comprises the application, to said plants or to the soils, of an effective amount of a fructooligosaccharide (FOS) comprising from 2 to 10 monosaccharide units or of a composition as has just been described.
  • FOS fructooligosaccharide
  • Another subject of the invention is a method for the biostimulation of plants which comprises the application, to said plants or to the soils, of an effective amount of a fructooligosaccharide (FOS) comprising from 2 to 10 monosaccharide units or of a product as described above.
  • FOS fructooligosaccharide
  • the present application aims to protect a method for treating plants or soils intended to activate reactions for eliciting mechanisms for absorption of inorganic nutrients, characterized in that it comprises the application, to said plants or to said soils, of an effective amount of FOS.
  • the product of the invention or the FOS may be applied to the plants via the leaves or via the roots.
  • the amount of FOS supplied to the plants is from 0.1 g to 100 g per litre, and preferentially of the order of 1 g per litre, when supplied in liquid form via the leaves or in nutritive solutions for the roots (hydroponically or dropwise).
  • the amount of FOS supplied to the plants is for example from 10 to 1000 g/ha, preferably of the order of 200 g/ha, when supplied in solid form, for example in pulverulent or granulated fertilizers.
  • the product of the invention may be used in one of the methods forming the subjects of the invention, at the following recommended doses:
  • the present invention can be used in the treatment of a very wide variety of plants. Among the latter, mention will in particular be made of:
  • plant is intended to denote, in the present application, the plant considered as a whole, including its root system, its vegetative system, the grains, seeds and fruits.
  • the present application aims to cover the use of FOSs comprising from 2 to 10 monosaccharide units to elicit mechanisms for absorption of inorganic nutrients in plants and as agent for stimulating natural plant defenses.
  • the inorganic nutrients will in particular be nitrogen, phosphorus and calcium.
  • FIG. 1 represents the concentration of salicylic acid (expressed as pmol/gPF) in the stems of young tobacco plants supplied with a nutritive solution containing a fructooligosaccharide in accordance with the invention (FOS) or the same nutritive solution without FOS (C).
  • FOS fructooligosaccharide in accordance with the invention
  • C the same nutritive solution without FOS
  • FIG. 2 represents the concentration of jasmonic acid (expressed as pmol/gPF) in the stems of young tobacco plants supplied with a nutritive solution containing a fructooligosaccharide in accordance with the invention (FOS) or the same nutritive solution without FOS (C).
  • FOS fructooligosaccharide in accordance with the invention
  • C the same nutritive solution without FOS
  • FIG. 3 represents the concentration of salicylic acid (expressed as pmol/gPF) in the stems of plants supplied with a nutritive solution containing a fructooligosaccharide not in accordance with the invention (Comparative FOS) or the same nutritive solution without FOS (Control).
  • FIG. 4 represents the concentration of jasmonic acid (expressed as pmol/gPF) in the stems of plants supplied with a nutritive solution containing a fructooligosaccharide not in accordance with the invention (Comparative FOS) or the same nutritive solution without FOS (Control).
  • the granules were dried in a drum dryer at 90° C. for 15 min.
  • the liquid containing the fructooligosaccharide was mixed with 10 kg of urea and 25 kg of monopotassium phosphate. The mixture was kept under continuous agitation at 25° C. for 24 h and the final product was filtered.
  • the tests were carried out on young, 15-day-old lettuce ( Lactuca sativa ) plants cultivated hydroponically. The tests were carried out in a greenhouse at temperatures of 22° C./16° C. day/night, 60% humidity and a photoperiod of 10 hours.
  • the lettuce seedlings (originating from a nursery) were washed to remove the compost around the root, and grown hydroponically for two days in water in order to remove the nutritive elements originating from the precultivation.
  • 9 young lettuce plants (three batches each comprising three young plants) were transferred to a fresh nutritive solution containing 100 mg.l ⁇ 1 of a fructooligosaccharide sold by Beghin Meiji under the reference Profeed®L95.
  • the nutritive solution contained: 2 mM of NO 3 ⁇ , 2 mM of PO 4 2 ⁇ , 4 mM of K + , 1 mM of Ca 2+ , 2 mM of Mg 2+ , 100 ⁇ M of Fe, 14 ⁇ M of B, 16 ⁇ M of Mn, 4 ⁇ M of Mo and 0.8 ⁇ M of Cu.
  • the pH of the solution was adjusted to 6 with KOH.
  • the amount of the inorganic elements absorbed was calculated by monitoring the depletion of these elements in the nutritive solution over a period of 30 hours.
  • the nitrate measurements were carried out by ion chromatography (Dionex 120) and the measurements of the other elements (P, Ca, Mg, K, Fe) were carried out by optical ICP (IRIS Intrepid II xdl, Thermo-Electron).
  • the plants were then harvested.
  • the root and the aerial part were separated, weighed then dried in a forced air oven at 45° C. for 48 h in order to determine their dry weight.
  • results underwent statistical analysis (ANOVA) using the STATISTICA 6.0 program, taking P ⁇ 0.05 as significant.
  • the degrees of root absorption of the nitrate, phosphate and calcium by the lettuces have been given in the following table. The results are expressed as % relative to the control (100%).
  • fructooligosaccharide in accordance with the invention The impact of a fructooligosaccharide in accordance with the invention and the impact of a fructooligosaccharide not in accordance with the invention on biostimulation were compared by measuring the content of jasmonic acid (plant hormone) in the stems of young tobacco plants.
  • jasmonic acid plays a role in resistance to insects and diseases.
  • An increase in the content of jasmonic acid in the tissues is therefore a good indicator of the increase in the potential of the young plants to resist pathogenic agents.
  • FOS fructooligosaccharide
  • oligosaccharides such as betamune (B), bio-mannanoligosaccharides (MS), mannanoligosaccharide (M), were tested either in aqueous solution (10 min at 100° C.) (B20, MS20, M20) or in acid solution (0.1N HCl, 10 min at 100° C., the pH of which was neutralized before application to the plants) (BCl, MSCl, MCl).
  • B betamune
  • MS bio-mannanoligosaccharides
  • M mannanoligosaccharide
  • the plants harvested following application of the treatment were frozen and ground with liquid nitrogen in order to carry out the quantitative analysis of the jasmonic acid and salicylic acid that they contain, according to the following protocol.
  • the jasmonic acid (JAS) and salicylic acid (SAL) standards were provided by Sigma-Aldrich (St Louis, USA).
  • the dihydrojasmonic acid (DHJAS) used as internal standard for the jasmonic add was provided by OlChemin Ltd (Olomouc, Czech Republic), and the 2 H 4 -salicylic acid (D-SAL) used as internal standard for the salicylic acid was provided by Sigma-Aldrich (St Louis, USA).
  • 0.5 ml of supernatant is introduced into a test tube already containing 0.5 ml of 0.4% acetic add, then this is agitated and centrifuged at 12000 min ⁇ 1 for 10 minutes. A portion of the supernatant is poured into a chromatography vial, from which it is injected into the LC/MS/MS system.
  • a Waters AllianceHT (Milford, Mass., USA) chromatograph is used, fitted with a 2795 XE separation module, an ALLCOLHTR column module with temperature regulation, and a 4 ⁇ m Hydro-RP Synergi (15 ⁇ 0.2 cm) chromatographic column (Phenomenex, Torrance, Calif., USA).
  • the gradient and the conditions used are as follows:
  • Injection volume 40 ⁇ l.
  • a 3200 Q TRAP LC/MS/MS System mass spectrometry detector (Applied Biosystems/MDS Sciex, Ontario, Canada) is used, fitted with a Turbo Ion Spray interface operating in negative mode.
  • GS1 nebulizer gas flow rate: 45.00 psi.
  • CAD collision-activated dissociation
  • the data obtained are processed by means of the Analyst 1.4.2 software from Applied Biosystems.
  • the values for limit of detection (LOD) and limit of quantification (LOQ) were calculated by taking 10 times the mean level of background noise in the blanks, and likewise in the plant samples.
  • the background noise was measured in the zones of the chromatogram close to the retention time of the hormones.
  • the results are expressed in ng/ml and in picograms injected and, in the case of the plant extracts, in ng/g of plant. The results are as follows:
  • LOD standards LOD plant LOQ standards LOQ plant Hormone ng/ml (pg) ng/g ng/ml (pg) ng/g JAS 0.15 (6) 1.20 0.45 (18) 3.50 SAL 0.10 (4) 1.00 0.30 (12) 3.00
  • a fructooligosaccharide comprising from 2 to 10 monosaccharide units in accordance with the invention induces a plant hormone concentration 4 to 8 times greater, depending on the tissues.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Agronomy & Crop Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)
  • Cultivation Of Plants (AREA)
US15/302,410 2014-04-09 2015-04-08 Fertilizing and bio stimulating product containing fructo oligosaccharides, application method and uses Abandoned US20170020132A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1453157 2014-04-09
FR1453157A FR3019715B1 (fr) 2014-04-09 2014-04-09 Composition fertilisante et biostimulante contenant des fructo-oligosaccharides, procede d'application et utilisations
PCT/FR2015/050917 WO2015155476A1 (fr) 2014-04-09 2015-04-08 Produit fertilisant et biostimulant contenant des fructo-oligosaccharides, procede d'application et utilisations.

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PCT/FR2015/050917 A-371-Of-International WO2015155476A1 (fr) 2014-04-09 2015-04-08 Produit fertilisant et biostimulant contenant des fructo-oligosaccharides, procede d'application et utilisations.

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EP (1) EP3128840B1 (ru)
BR (1) BR112016023458B1 (ru)
ES (1) ES2933905T3 (ru)
FR (1) FR3019715B1 (ru)
HR (1) HRP20221365T1 (ru)
HU (1) HUE060565T2 (ru)
PL (1) PL3128840T3 (ru)
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Cited By (1)

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Publication number Priority date Publication date Assignee Title
US20170233302A1 (en) * 2016-01-28 2017-08-17 R. Umar Hasan Saputra Process for producing coal-based fertilizer and the products produced

Families Citing this family (1)

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WO2024130376A1 (pt) * 2022-12-23 2024-06-27 Yessinergy Holding S.A. Composição para promover a multiplicação e a sobrevida de microrganismos, método de tratamento de plantas e uso da composição em biodefensivos agricolas

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5733579A (en) * 1995-04-05 1998-03-31 Abbott Laboratories Oral rehydration solution containing indigestible oligosaccharides
AU6889698A (en) * 1997-04-11 1998-11-11 Auxein Corporation Compositions and methods utilizing succinate and raffinates to improve plant growth
JPH10298020A (ja) * 1997-04-22 1998-11-10 Gun Ei Chem Ind Co Ltd 作物栄養補助剤及び該作物栄養補助剤を用いた作物栽培方法
FR2766059B1 (fr) * 1997-07-18 1999-09-17 Goemar Lab Sa Procede pour la stimulation des defenses naturelles de plantes agronomiquement utiles et composition pour la mise en oeuvre de ce procede
FR2836011B1 (fr) * 2002-02-20 2004-05-14 Goemar Lab Sa Agent pour la stimulation des defenses naturelles des plantes et procede pour sa mise en oeuvre
FR2868253B1 (fr) * 2004-03-30 2006-07-21 Cie Financiere Et De Participa Utilisation des ulvanes comme activateurs des reactions de defense des plantes et de resistance contre des contraintes biotiques ou abiotiques
JP2007215462A (ja) * 2006-02-16 2007-08-30 National Agriculture & Food Research Organization ミネラル吸収促進物質を含有する食用植物体およびその製造方法
CN100486434C (zh) 2007-01-11 2009-05-13 山东应天生物科技有限公司 牛蒡低聚果糖在农业上作为抗病诱导子的应用
KR101670092B1 (ko) * 2008-06-12 2016-10-27 티엔세 수이케라피나데리예 엔.브이. 프럭토올리고당 조성물, 이것의 제조 방법 및 용도
US20110294864A1 (en) * 2009-01-13 2011-12-01 Universiteit Gent Aqueous coacervate compositions suitable for making powders and water-soluble formulations of biologically-active agents

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170233302A1 (en) * 2016-01-28 2017-08-17 R. Umar Hasan Saputra Process for producing coal-based fertilizer and the products produced
US10683243B2 (en) * 2016-01-28 2020-06-16 R. Umar Hasan Saputra Process for producing coal-based fertilizer and the products produced

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PL3128840T3 (pl) 2023-01-09
FR3019715B1 (fr) 2018-04-06
ES2933905T3 (es) 2023-02-14
EP3128840A1 (fr) 2017-02-15
WO2015155476A1 (fr) 2015-10-15
BR112016023458B1 (pt) 2022-06-07
PT3128840T (pt) 2022-10-21
EP3128840B1 (fr) 2022-09-28
BR112016023458A2 (ru) 2017-08-15
HUE060565T2 (hu) 2023-03-28
FR3019715A1 (fr) 2015-10-16
HRP20221365T1 (hr) 2023-01-06
US20190183120A1 (en) 2019-06-20

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