US20140219311A1 - Isothermal titration microcalorimeter apparatus and method of use - Google Patents
Isothermal titration microcalorimeter apparatus and method of use Download PDFInfo
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- US20140219311A1 US20140219311A1 US14/253,168 US201414253168A US2014219311A1 US 20140219311 A1 US20140219311 A1 US 20140219311A1 US 201414253168 A US201414253168 A US 201414253168A US 2014219311 A1 US2014219311 A1 US 2014219311A1
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- G—PHYSICS
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- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K17/00—Measuring quantity of heat
- G01K17/006—Microcalorimeters, e.g. using silicon microstructures
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- G—PHYSICS
- G01—MEASURING; TESTING
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- G01N25/20—Investigating or analyzing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity
- G01N25/48—Investigating or analyzing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity on solution, sorption, or a chemical reaction not involving combustion or catalytic oxidation
- G01N25/4846—Investigating or analyzing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity on solution, sorption, or a chemical reaction not involving combustion or catalytic oxidation for a motionless, e.g. solid sample
- G01N25/4866—Investigating or analyzing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity on solution, sorption, or a chemical reaction not involving combustion or catalytic oxidation for a motionless, e.g. solid sample by using a differential method
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1081—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane
- G01N35/109—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane with two horizontal degrees of freedom
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1048—General features of the devices using the transfer device for another function
- G01N2035/1058—General features of the devices using the transfer device for another function for mixing
Abstract
An automatic pipette assembly for an isothermal titration micro calorimetry system, comprising a pipette housing, a syringe with a titration needle arranged to be inserted into a sample cell for supplying titrant, and a linear activator for driving a plunger in the syringe, the titration needle is rotatable with respect to the housing and is provided with a stirring paddle arranged to stir fluid in the sample cell, wherein the automatic pipette assembly comprises a stirring motor for driving the rotation of the titration needle. There is also provided an isothermal titration micro calorimetry system.
Description
- This application claims priority to U.S. provisional patent application No. 60/984,432 filed Nov. 1, 2007; the disclosure of which is incorporated herein by reference in its entirety.
- 1. Field of the Invention
- The present invention relates generally to microcalorimeters and more specifically to features that improve the performance of microcalorimeters, especially isothermal titration calorimeters.
- 2. Background
- Microcalorimeters are broadly utilized in fields of biochemistry, pharmacology, cell biology, and others. Calorimetry provides a direct method for measuring changes in thermodynamic properties of biological macromolecules. Microcalorimeters are typically two cell instruments in which properties of a dilute solution of test substance in an aqueous buffer in a sample cell are continuously compared to an equal quantity of aqueous buffer in a reference cell. Measured differences between the properties of the two cells, such as temperature or heat flow, are attributed to the presence of the test substance in the sample cell.
- One type of microcalorimeter is an isothermal titration calorimeter. The isothermal titration calorimeter (ITC) is a differential device, but operates at a fixed temperature and pressure while the liquid in the sample cell is continuously stirred. The most popular application for titration calorimetry is in the characterization of the thermodynamics of molecular interactions. In this application, a dilute solution of a test substance (e.g., a protein) is placed in the sample cell and, at various times, small volumes of a second dilute solution containing a ligand, which binds to the test substance, are injected into the sample cell. The instrument measures the heat which is evolved or absorbed as a result of the binding of the newly-introduced ligand to the test substance. From results of multiple-injection experiments, properties, such as, the Gibbs energy, the association constant, the enthalpy and entropy changes, and the stoichiometry of binding, may be determined for a particular pairing between the test substance and the ligand.
- While currently utilized ITCs provide reliable binding data results, their widespread utilization in the early stages of drug development have been limited by several factors: the relatively high amounts of protein required to perform a binding determination (e.g., about 0.1 milligram (mg) to about 1.0 mg of a protein), the time required to perform the measurement, and the complexity of using conventional ITCs. Due to the extremely high costs of biological substances used in research, there is a need to reduce the amount of biological substance used for each experiment. A reduction in the amount of the biological substance used in a calorimeter experiment, will require a more accurate, sensitive, and reliable titration calorimeter than what is currently available.
- Furthermore, gathering binding data utilizing prior art ITCs require extensive preparation and skill by the practitioner. For example, using prior art ITCs, the reference and sample cells are first filled respectively with the reference substance and sample substance via a corresponding cell stem. Next, a syringe of the ITC is filled with a titrant. Then a needle of the ITC is placed in the sample cell via a cell stem leading to the sample cell while the syringe fits into a holder on the ITC enabling the syringe to rotate around its axis. Subsequently, the syringe is aligned with the sample cell so that the needle does not touch either the cell stem or the sample cell. Then, the syringe is connected to a stirring motor and a linear activator of the ITC, wherein the stirring motor and the linear activator must also be aligned with the sample cell.
- As would be appreciated by a reading of the above-described prior art procedure, utilizing prior art ITCs, the quality of binding measurements performed with these prior art ITCs depends heavily of the operator's skills and experience, and involves a considerable amount of preparation time.
- More recently developed prior art ITCs have attempted to simplify the preparatory procedures described above. For example, in one such prior art ITC, which is partly shown in
FIG. 1 as ITC 100, asyringe 102, asyringe holder 104, and alinear actuator 106, which actuatessyringe 102's plunger, are integrated into a single unit referred to as an automatic pipette. ITC 100 further comprises a stirring mechanism comprising a stirringmotor 108, which is attached tocalorimeter body 110. ITC 100 also comprises aninner magnet couple 112 located aroundsyringe 102, and anouter magnet couple 114 located oncalorimeter body 110 in close proximity to stiffingmotor 108. The rotation from stirringmotor 108 tosyringe 102 is transferred viamagnet couplings syringe 102 is aneedle 116 and apaddle 118. Theneedle 116 is arranged to be inserted into asample cell 120 via acell stem 122 for performing ITC experiments. For reference purposes,ITC 100, also comprises a reference cell, not shown, in communication with the ambient atmosphere via a reference cell stem. - The prior art design discussed above and depicted in
FIG. 1 has certain limitations. For example, since the magnet coupling is a soft/flexible transmission, it is prone to resonant vibration of the stirrer at certain rotation speeds and accelerations, which negatively affects the instrument's sensitivity. The resonant vibration can be reduced by either employing a less sensitive feedback mechanism controlling the rotation speed (which leads to less stable rotation speed), or by lowering the rotation speed. However, less stable rotation speed also reduces the ITC's sensitivity, while lower rotation speed impedes proper mixing of reagents which reduces the ITC's accuracy. - Another limitation of the prior art design is that the stirring motor and the magnet coupling are placed closely to the sensitive measuring unit of the instrument and generates a substantial alternating magnetic field that produces electric noise which negatively affects the operation of the ITC's sensitive electronic circuitry. Since the ITC's sensors process signals of approximately 10−9 volts, and the noise generated by the motor and the magnetic coupling is a reciprocal of the distance between the sensor and the source of the noise, further improvements in the performance characteristics of this ITC design become increasingly challenging. As stated earlier, one of the underlying factors affecting the design of new microcalorimeters is the need to reduce the amount of biological substance used for each experiment. This requires smaller sample cells and shorter cell stems which in turn leads to, smaller distances between the cell sensor and the motor and magnetic coupling (source of electric noise), which limits the instrument's sensitivity.
- The invention described herein is aimed to improve the aforementioned characteristics and use of prior art ITCs such that the sensitivity of the ITC is improved, the amount of biological substance necessary for testing is reduced, the reliability of the results generated by the ITC is improved, and use of the ITC is eased.
- The object of the invention is to provide a new automatic pipette assembly for an isothermal titration micro calorimetry system and an ITC system, which pipette assembly and ITC system overcomes one or more drawbacks of the prior art. This is achieved by the pipette assembly and the ITC system as defined in the independent claims.
- One advantage with the present pipette assembly and the associated ITC system is that it makes it possible to reduce the cell compartment volume by about a factor of seven as compared to prior art ITCs, without a reduction in sensitivity, and with a significantly faster response time. Such an ITC system permits the performance of experiments with about 10 times less protein sample, and with only a total of about 2 to about 4 titrations per hour.
- In addition to reducing the costs associated with running the ITC experiment, a smaller cell volume also extends the number of ITC applications. For example, the range of binding affinities that can be measured by ITC is dictated by a parameter called “c value,” which is equal to the product of the binding affinity (Ka) and the total concentration (Mtotal) of macromolecule (c=[Mtotal]Ka). For accurate affinity determination, the c value must be between 1 and 1,000. A decrease in the cell volume by a factor of ten results in a similar increase in c value if the same amount of protein is used, and, consequently, the ability to measure weak binders. This ability is especially important in the early stages of drug discovery, in which binding affinities are weak, especially in conjunction with a fully automated instrument.
-
FIG. 1 shows a schematic example of a prior art isothermal titration microcalorimeter highlighting the automatic pipette feature of the microcalorimeter; -
FIG. 2 shows, in cross-section, a schematic embodiment of an isothermal titration calorimeter system of the present invention with a guiding mechanism; -
FIG. 3 shows, in cross-section, a schematic embodiment of an automatic pipette assembly for an isothermal titration micro calorimetry system; -
FIG. 4 shows a section of the ITC system shown inFIG. 2 , wherein the pipette assembly is retracted from the sample cell; -
FIGS. 5 a to 5 c show a schematic embodiment of a guiding mechanism for an isothermal titration calorimeter system in perspective; -
FIG. 6 shows another schematic embodiment of a guiding mechanism for an isothermal titration calorimeter system in perspective. -
FIG. 2 schematically shows one embodiment of anITC system 200 according to the present invention. The ITCsystem 200 comprises amicro calorimeter 210 and anautomatic pipette assembly 220. Themicro calorimeter 210 comprises areference cell 240 and asample cell 250 which are designed to be essentially identical in heat capacity and volume. Thecells cells cells - In order to reduce any external thermal influences to a minimum, the,
reference cell 240 and thesample cell 250 are both enclosed by a firstthermal shield 260 which in turn is enclosed by a secondthermal shield 270. Thethermal shields shields calorimetric cells - In order to control the temperature of the
shields thermal shields - In the disclosed embodiment a first
heat pump unit 280 is arranged to transfer heat energy between the first 260 and secondthermal shields 270, a secondheat pump unit 290 is arranged to transfer heat energy between the secondthermal shield 270 and aheat sink 300 in thermal contact with the ambient temperature. Atemperature controller 310 is arranged to control the first and secondheat pump units temperature controller 310 and associated sensors will be disclosed in more detail below. - A
reference cell stem 320 and asample cell stem 330 provides access to thereference cell 240 andsample cell 250, respectively, for supplying reference and sample fluids, titration fluid, washing of the cells etc. In the disclosed embodiment, the cell stems 320 and 330 both extends essentially vertically through both thermal shields and the heat sink to provide direct communication withcells respective cell thermal shield 260. - The
automatic pipette assembly 210 comprises apipette housing 340, asyringe 350 with atitration needle 360 arranged to be inserted into thesample cell 250 for supplying titrant, and alinear activator 370 for driving aplunger 380 in thesyringe 350. Thetitration needle 360 is rotatable with respect to thehousing 340 and is provided with a stirringpaddle 390 arranged to stir sample fluid in thesample cell 250 in order to achieve efficient mixing of titrant and sample fluid. Theautomatic pipette assembly 210 further comprises a stirringmotor 400 for driving the rotation of thetitration needle 360. - In the embodiment disclosed in
FIG. 2 the stiffingmotor 400 is a direct drive motor with ahollow rotor 410 arranged concentric with thesyringe 350 and thetitration needle 360. Thesyringe 350 is at its upper end supported for rotation by the stiffingmotor 400 and at the lower end by abearing 420. Both the stirringmotor 400 and thebearing 420 are schematically disclosed as comprising ball bearings, but any other type of bearing, bushings or the like capable of providing smooth and low friction rotation of thetitration needle 360 may be used. - In the embodiment disclosed in
FIG. 2 , thesyringe 350 is arranged to be rotatable with respect to thehousing 340 and thetitration needle 360 is non-rotatably attached to thesyringe 350. In an alternative embodiment (not shown in figs.), theneedle 360 is rotatable with respect to thesyringe 350 and thesyringe 350 static with respect to thepipette housing 340.FIG. 3 shows an alternative embodiment, wherein thesyringe 350 is detachably arranged in arotation frame 430 within thepipette housing 340, therotation frame 430 being rotably supported by themotor 400 andbearing 420. In the disclosed embodiment, thesyringe 350 is retained in therotation frame 430 by acap 440 that is detachably attached to therotation frame 430 by threads or the like. By removing thecap 440, thesyringe 350 with thetitration needle 360 can be replaced. - In an alternative embodiment, not shown in the figures, the stirring
motor 400 drives the titration needle for rotation by a rotation transmission arrangement, such as a drive belt arrangement, a drive wheel arrangement or the like. In such an arrangement, the drive motor may be placed at an ever greater distance from the calorimetric cells. - The stirring motor is controlled by a
stir controller 450 of the ITC system. The stir controller be a conventional BLDC. The linear actuator is controlled by atitration controller 460 of the ITC system. The stir andtitration controllers pipette assembly 220, and in turn connected to the ITC control system (not shown in detail), or they may be an integrated part of the ITC control system. - In the disclosed embodiment, the
linear activator 370 comprises astepper motor 470 arranged to drive a threadedplunger 380 that extends coaxially through the hole of ahollow rotor 480 and into thesyringe 350 wherein it is rotatably attached to apipette tip 490 that seals against the inner wall of thesyringe 350 to allow displacing a precise volume of titration liquid fromsyringe 350. The pipette assembly further comprisesposition sensors plunger 380. Thelinear activator 370 may be of any other type capable of perform controlled linear motion with sufficient precision. This design allows syringe to be rotated independently of themain body 340 of thepipette assembly 220; at the same time, thelinear activator 370 can drive the threadedplunger 380. - In the ITC system the
titration 460 controller is arranged to control the linear activator of thepipette assembly 220. According to one embodiment,titration controller 460 uses theposition sensors titration controller 460 is arranged to register the two predetermined positions of the threadedplunger 380 and to determine the pitch of the threads of theplunger 380 from the number of steps performed by thestepper motor 470 to move the threadedplunger 380 between said positions. The so determined pitch of the threaded plunger is thereafter used by the pipette controller to increase the accuracy of the titration pipette, when displacing small volumes of titrant. - In the disclosed embodiments, the
pipette assembly housing 340 serves as mounting base for the stators of the stirringmotor 400 and thelinear activator 470. Thehousing 340 may further comprise an attachment section for precise positioning of the pipette assembly with respect to the sample cell stem. - The
automatic pipette assembly 220 with anintegrated stiffing motor 400 arranged at the upper end of, or above thesyringe 350 not only increases the distance between the stirring motor 400 (i.e., the source of an alternating electro-magnetic field) and the sensitive electronic circuitry, which is located at and/or nearby the thermal core of the calorimeter, but it also allows for the reduction in the amount of power needed to operate the stirringmotor 400. That is, due to the placement of the stiffingmotor 400 on thepipette assembly 220, the size of the stirringmotor 400 is not determined by the space between the sample cell and the reference cell as it is determined by prior art ITCs that place the stiffing motor on the calorimeter housing (FIG. 2 ). In the prior art, then, the cell stems protrude through the center of rotation of the magnetically coupled stirrer. Therefore, the lower limit of size for the stirrer mechanism is determined by the spacing between the cell stems. Accordingly, the size of the stiffing motor of the present invention can be significantly reduced, e.g., by a factor of about 5 times that of stiffing motors found in prior art ITCs, thereby resulting in a significant reduction in the amount of power needed to operate the ITC, e.g., about 2 watts versus the 100 watts used to power conventionally known ITCs. - The reduction in size of the stirring motor to a size of about five times less than stiffing motors of prior art ITCs, and the placement of the stirring motor on the pipette such that the stirring motor is at a distance of about 50 millimeters or more away from the sensitive electronic circuitry of the calorimeter, and the removal of the magnetic coupling, closes the magnetic field that exists in conventionally used ITCs. Accordingly, the sensitivity of the inventive ITC is raised by the lower power, the lower heat, the lower electricity, and the lower noise and vibration caused by the placement of the stirring motor as disclosed herein. Furthermore, the disclosed embodiments also exclude magnetic coupling which is an additional source of alternating electro-magnetic field.
- These improvements reduce the electrical noise induced in the sensitive electronic circuitry which, in turn, helps improve the calorimeter's sensitivity, whereby the volume of the sample and reference cells may be significantly reduced. Reducing the size of the sample cell and its corresponding cell stem, in turn, reduces the amount of biological substance used for each experiment.
- The following Table 1 represents certain key dimensions of the cell stems and test and reference cells of both the prior art ITC and the ITC of the present invention. As shown in Table 1, the inventive ITC, which places the stirring motor directly on the pipette subassembly, rather than positioning it on the calorimeter body, allows a smaller volume of test substance to be used, thereby reducing the costs associated with conducting calorimeter experiments.
-
TABLE 1 Prior Art ITC Inventive ITC Stem Interior Diameter 2.6 millimeters 2.0 millimeters Cell stem Length 72.0 millimeters 40.5 millimeters Cell stem Volume 382.0 microliters 127.0 microliters Cell Interior Diameter 22.0 millimeters 11.0 millimeters Cell Thickness 3.7 millimeters 2.2 millimeters Cell Volume 1,400 microliters 200 microliters - According to one embodiment, the micro
titration calorimetry system 200 is provided with apipette guiding mechanism 510 arranged to guide thepipette assembly 220 between and into at least two positions of operation. The first position of operation is apipette washing position 580 wherein thetitration needle 360 is inserted in a washing apparatus (seeFIGS. 5 a-5 c), and the second position of operation is atitration position 560 wherein the syringe is inserted into thesample cell 250 for calorimetric measurements. In the embodiment ofFIG. 2 , theguiding mechanism 510 is comprised of apipette arm 520 that supports thepipette assembly 220, and an essentiallyvertical guide rod 530. Thepipette arm 520 is moveably attached by asleeve 540 to theguide rod 530, but its motion about the guide rod is restricted by aguide groove 550 in theguide rod 530 and aguide pin 560 that protrudes from the inner surface of thesleeve 540 and which fits into theguide groove 550. The disclosedguiding mechanism 510 is of rotational type, and the positions of operation are arranged at equal distance about the centre of rotation of the guide assembly but at different angular positions, wherein movement of thepipette assembly 220 in the vertical direction is restricted to the angular positions of the positions of operation, and wherein rotational movement of thepipette assembly 220 between the angular positions only is permitted when thetitration needle 260 is fully retracted from respective positions of operation.FIG. 4 shows theguide mechanism 510 in a position wherein thepipette assembly 220 is fully retracted from the titration position, i.e. from thesample cell 250. At this position theguiding mechanism 510 restricts the possible movement of thepipette arm 520 to a vertical movement down into the titration position, or a rotational movement to reach another position of operation. In a microtitration calorimetry system 200 with two positions of operation, thewashing apparatus 600 may be arranged to allow filling of titrant to thepipette assembly 220 after washing of the pipette assembly is completed. Alternatively, filling of the pipette may be performed through other means, such as a specific filling port in the syringe or the like. -
FIGS. 5 a to 5 c show a schematic perspective view of one embodiment of a microtitration calorimetry system 200 with apipette guiding mechanism 510, arranged to guide thepipette assembly 220 to and from three different positions ofoperation titrant filling position 570, wherein thetitration needle 360 is inserted in atitrant source 590.FIG. 5 a shows theguiding mechanism 510 in a state wherein thepipette assembly 220 is in thetitration position 560.FIG. 5 b shows theguiding mechanism 510 in a state intermediate thetitration position 560 and the fillingposition 570.FIG. 5 c shows theguiding mechanism 510 in a state wherein thetitration needle 360 of thepipette assembly 220 is in thefilling position 570. As is disclosed inFIGS. 4 a to 5 c, thevertical guide grooves 550 associated with a specific position ofoperation pipette assembly 220 at a predetermined height with respect to the operation to be performed. -
FIG. 6 shows another embodiment of theguide mechanism 510, wherein the guide groove in theguide rod 530 is replaced by a coaxialexternal guide sleeve 610 withcorresponding guide paths 620 for theguide arm 520. - According to one embodiment, the micro
titration calorimetry system 200 comprises one or more position sensors, not shown, arranged to register when thepipette assembly 220 is positioned at one or more of the positions ofoperation pipette assembly 220 is in correct position fortitration 560, and thecalorimetry system 200 may be arranged to prevent start of a titration operation unless said sensor confirms that thepipette assembly 220 is in correct position. - Again referring to the figures, the
guiding mechanism 510 ensures proper alignment and positioning ofpipette assembly 220 in thesample cell 250 etc. All elements of the pipette assembly 220 (syringe, needle, paddle, plunger, linear activator, hollow rotor) and theguiding mechanism 510 are preferably precisely aligned during the manufacturing process and do not require any additional alignment during the use of the instrument. The pre-set factory alignment significantly improves usability and reliability of the instrument, considerably reduces the amount of preparatory time for an experiment, and makes the quality of measurements independent of the user skills. Theguiding mechanism 510 also enables proper positioning of thepipette assembly 220 in thewashing apparatus 600 for cleaning and drying of the syringe and e.g. for filling the syringe with titrant. - An exemplary method for utilizing the ITC apparatus disclosed herein comprises the non-manual alignment, both in depth and in breadth, of the syringe for filling titrant, washing the pipette, and delivering the titrant to the syringe.
- An exemplary method for operating the inventive ITC apparatus comprises using the
guiding mechanism 510 to position the pipette for filling the syringe with titrant (seeFIG. 5 c). In this position, the end of the needle with the paddle is placed in the titrant. The plunger of the syringe is moved from its lower position to its upper position thereby filling the syringe with the titrant. The sample cell is filled with the sample solution via the cell stem using an auxiliary syringe. Using the guiding mechanism, the pipette is moved to a position for performing an experiment (seeFIG. 5 a). The program for performing the experiment is activated. Consistent with the program used for the experiment, the rotor of the stirring motor rotates the syringe, needle, and paddle with the assigned speed enabling proper mixing of the reagents. Consistent with the program used for the experiment (e.g., when a certain temperature and/or equilibrium are reached), the linear activator moves the plunger and injects the titrant into the sample solution. The injection can be done discretely (step-by-step) or continuously, depending on the program settings. The calorimeter continuously measures and records the heat release/absorption versus time associated with the interaction of reagents. The analysis of the results is done according to the established algorithm. - Referring again to
FIG. 2 , thetemperature controller 310 is arranged to control the first and secondheat pump units temperature controller 310 is schematically disclosed by functional blocks, and it may either be designed as an electronic circuit, as a software in a CPU based controller, or as a combination thereof. In the disclosed embodiment, thethermal controller 310 may be switched between two modes of operation: -
- Isothermal mode
- Thermal set mode
by means of a modeselect block 630.
- In the isothermal mode the
thermal controller 310 is arranged to control thefirst heat pump 280 to minimize the temperature difference between thesample cell 250 and the firstthermal shield 260. The temperature difference between thesample cell 250 and the firstthermal shield 260 is registered by atemperature sensor arrangement 640 in communication with thetemperature controller 310. In the disclosed embodiment, thetemperature sensor arrangement 640 is a differential thermocouple that gives a non-zero signal when there is a temperature difference between the two points of registration, and the output from the thermocouple is connected to apreamplifier block 650 in thetemperature controller 310. The out-put from thepreamplifier 650 is directed by the modeselect block 630 to a first heatpump controller block 660 that controls thefirst heat pump 280 in response to the signal from thepreamplifier 650. By this arrangement, the firstheat pump controller 660 will strive to compensate for any thermal difference between thesample cell 250 and the firstthermal shield 260, whereby a compensated adiabatic state is achieved in thecalorimeter 210. In order to compensate for minor systematic drifts in adiabatic behavior, an offsetparameter 670 may be applied on the signal from thepreamplifier 650. The offsetparameter 670 may be set during calibration of thecalorimeter 210. In the isothermal mode, thetemperature controller 310 is arranged to control thesecond heat pump 290 to keep the secondthermal shield 270 at a predetermined temperature, as is defined by a parameter Tshield. The control of thesecond heat pump 290 is performed by a second heatpump controller block 680 that receives an output from asecond comparator block 690 wherein Tshield is compared with the present temperature of the second shield registered by a second shieldthermal sensor 700. In the temperature set mode thetemperature controller 310 is arranged to control the first andsecond heat pump thermal shields calorimeter 210 to the temperature at which the ITC experiments are to be run. In this mode, afirst comparator block 710 compares Tshield with the present temperature of the first shield registered by a first shieldthermal sensor 720, and the output from thefirst comparator block 710 is directed by the modeselect block 630 to the firstheat pump controller 660. Thesecond heat pump 290 is controlled in the same manner as in the isothermal mode. The thermal set mode may further be used as a stand by mode in order to keep a constant temperature in thecalorimeter 210. - The
temperature controller 310 may further comprise a cell temperature controller block 730 for controllingcell heating elements 740 arranged to heat the sample and thereference cells cell heating elements 740, are mainly used for fine tuning of the temperature of thecells - The parameters Tshield, Tcell and offset may be set via a dedicated user interface of the thermal controller, not shown, or e.g. via a calorimeter user interface run on a
computer 760 or the like.calorimetric sensors 750 for sensing the temperature difference between thesample cell 250 andreference cell 240 during the ITC experiments may be connected to thecomputer 760, e.g. via apreamplifier 770. - With respect to the above description, it is to be realized that the optimum dimensional relationships for the parts of the invention, to include variations in size, materials, shape, form, function and manner of operation, assembly and use, are deemed readily apparent and obvious to one skilled in the art, and all equivalent relationships to those illustrated in the drawings and described in the specification are intended to be encompassed by the present invention. Therefore, the foregoing is considered as illustrative only of the principles of the invention. Further, since numerous modifications and changes will readily occur to those skilled in the art, it is not desired to limit the invention to the exact construction and operation shown and described, and accordingly, all suitable modifications and equivalents may be resorted to, falling within the scope of the invention.
Claims (11)
1-21. (canceled)
22. A micro titration calorimetry system comprising a pipette assembly with a titration needle arranged to be inserted into a sample cell for supplying titrant, and a pipette guiding mechanism arranged to restrict the movement of the pipette assembly along safe paths to ensure that the titration needle cannot be damaged during movement thereof between different positions of operation.
23. The micro titration calorimetry system of claim 22 , wherein the pipette guiding mechanism is arranged to guide the pipette assembly between and into at least two positions of operation, wherein a first position of operation is a pipette washing position wherein the titration needle is inserted in a washing apparatus, and a second position of operation is a titration position wherein the syringe is inserted into the sample cell for calorimetric measurements.
24. The micro titration calorimetry system of claim 22 , wherein the washing apparatus is arranged to allow filling of titrant to the pipette assembly after washing of the pipette assembly is completed.
25. The micro titration calorimetry system of claim 22 , further comprising a third position of operation in the form of a titrant filling position, wherein the titration needle is inserted in a titrant source.
26. The micro titration calorimetry system of claim 23 , wherein the guiding mechanism is of rotational type, and the positions of operation are arranged at equal distance about the centre of rotation of the guide assembly but at different angular positions, wherein movement of the pipette assembly in the vertical direction is restricted to the angular positions of the positions of operation, and wherein rotational movement of the pipette assembly between the angular positions only is permitted when the needle is fully retracted from respective positions of operation.
27. The micro titration calorimetry system of claim 22 , further comprising a position sensor arranged to register when the pipette assembly is positioned at one of the positions of operation, and wherein the associated operation is restricted by the state of the position sensor.
28. A pipette assembly for an isothermal titration micro calorimetry system, comprising a titration needle arranged to be inserted into a sample cell for supplying titrant, the titration needle is rotatable with respect to the micro calorimetry system and is provided with a stirring paddle arranged to stir fluid in the sample cell, wherein the pipette assembly comprises a stirring motor for driving the rotation of the titration needle.
29. The automatic pipette assembly of claim 28 , wherein the stirring motor is a direct drive motor with a hollow rotor arranged concentric with the titration needle.
30. The automatic pipette assembly of claim 28 , wherein the stirring motor drives the titration needle for rotation by a rotation transmission arrangement.
31. An isothermal titration micro calorimetry system with the automatic pipette assembly of claim 28 .
Priority Applications (1)
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US14/253,168 US20140219311A1 (en) | 2007-11-01 | 2014-04-15 | Isothermal titration microcalorimeter apparatus and method of use |
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US98443207P | 2007-11-01 | 2007-11-01 | |
PCT/US2008/081961 WO2009059110A1 (en) | 2007-11-01 | 2008-10-31 | Isothermal titration microcalorimeter apparatus and method of use |
US74038010A | 2010-04-29 | 2010-04-29 | |
US13/780,536 US8827549B2 (en) | 2007-11-01 | 2013-02-28 | Isothermal titration microcalorimeter apparatus and method of use |
US14/253,168 US20140219311A1 (en) | 2007-11-01 | 2014-04-15 | Isothermal titration microcalorimeter apparatus and method of use |
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US13/780,536 Continuation US8827549B2 (en) | 2007-11-01 | 2013-02-28 | Isothermal titration microcalorimeter apparatus and method of use |
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US13/780,536 Active US8827549B2 (en) | 2007-11-01 | 2013-02-28 | Isothermal titration microcalorimeter apparatus and method of use |
US14/253,168 Abandoned US20140219311A1 (en) | 2007-11-01 | 2014-04-15 | Isothermal titration microcalorimeter apparatus and method of use |
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US13/780,536 Active US8827549B2 (en) | 2007-11-01 | 2013-02-28 | Isothermal titration microcalorimeter apparatus and method of use |
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US (3) | US8449175B2 (en) |
EP (1) | EP2208057A4 (en) |
JP (1) | JP5542678B2 (en) |
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WO (1) | WO2009059110A1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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US9702793B2 (en) | 2015-03-16 | 2017-07-11 | Todd A Balisky | Variable volume sample capture device |
US20190003995A1 (en) * | 2016-01-14 | 2019-01-03 | Jamlet MONASELIDZE | Differential scanning microcalorimeter device for detecting disease and monitoring therapeutic efficacy |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9103782B2 (en) * | 2008-12-02 | 2015-08-11 | Malvern Instruments Incorporated | Automatic isothermal titration microcalorimeter apparatus and method of use |
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JP2015526720A (en) | 2012-07-24 | 2015-09-10 | ザ トラスティーズ オブ コロンビア ユニバーシティ イン ザ シティオブ ニューヨーク | MEMS-based isothermal titration calorimetry |
US10337933B2 (en) | 2015-04-17 | 2019-07-02 | Waters Technologies Corporation | Stirring paddle for isothermal titration calorimetry |
RU2610853C1 (en) * | 2016-01-26 | 2017-02-16 | Федеральное государственное бюджетное учреждение науки Институт биологического приборостроения с опытным производством Российской Академии наук (ИБП РАН) | Capillary titration calorimeter for investigating mitochondria |
RU2618670C1 (en) * | 2016-03-21 | 2017-05-05 | Федеральное государственное бюджетное учреждение науки Институт биологического приборостроения с опытным производством Российской Академии наук (ИБП РАН) | Capillary titration nano-calorimeter for researching mitochondria |
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RU2713808C1 (en) * | 2018-09-04 | 2020-02-07 | Федеральное государственное бюджетное учреждение науки "Федеральный исследовательский центр "Пущинский научный центр биологических исследований Российской академии наук" (ФИЦ ПНЦБИ РАН) | Cooling and heating method with constant speed of extended calorimetric chambers of isothermal capillary differential titration nanocalorimeter intended for operation with short-lived objects |
US11047748B1 (en) * | 2020-08-14 | 2021-06-29 | Frank L. Wu | Adiabatic power compensation differential scanning calorimeter |
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Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4259843A (en) * | 1979-10-09 | 1981-04-07 | Cromemco Inc. | Isolation chamber for electronic devices |
US4643879A (en) * | 1985-07-01 | 1987-02-17 | American Hospital Supply Corporation | Tower for analyzing system |
US4681741A (en) * | 1985-07-01 | 1987-07-21 | American Hospital Supply Corporation | Reagent dispenser for an analyzing system |
EP0266155A2 (en) * | 1986-10-24 | 1988-05-04 | Richard W. Hanaway | Multiple pipette sampler |
US5000921A (en) * | 1986-10-24 | 1991-03-19 | Hanaway Richard W | Multiple pipette samples |
US5096670A (en) * | 1986-10-31 | 1992-03-17 | Harris Paul C | Automated patient sample analysis instrument |
US5813763A (en) * | 1996-10-11 | 1998-09-29 | Microcal Incorporated | Ultrasensitive differential microcalorimeter |
US6395231B1 (en) * | 1997-10-21 | 2002-05-28 | Cybio Instruments Gmbh | Pipette and handling automatic machine for microtitration plates with permeable bases |
US20030032171A1 (en) * | 2001-08-08 | 2003-02-13 | Dade Behring Inc. | Automated random access microbiological analyzer |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63500117A (en) | 1985-07-01 | 1988-01-14 | バクスター・ダイアグノスティックス・インコーポレイテッド | Reagent dosing device for analyzers |
JP3990944B2 (en) * | 2002-06-28 | 2007-10-17 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
JP4327760B2 (en) * | 2005-03-29 | 2009-09-09 | シチズンホールディングス株式会社 | Calorimeter and analysis method using the same |
-
2008
- 2008-10-31 WO PCT/US2008/081961 patent/WO2009059110A1/en active Application Filing
- 2008-10-31 US US12/740,380 patent/US8449175B2/en active Active
- 2008-10-31 EP EP08843953.4A patent/EP2208057A4/en active Pending
- 2008-10-31 JP JP2010532272A patent/JP5542678B2/en active Active
- 2008-10-31 CN CN2008801148263A patent/CN101855541B/en active Active
-
2013
- 2013-02-28 US US13/780,536 patent/US8827549B2/en active Active
-
2014
- 2014-04-15 US US14/253,168 patent/US20140219311A1/en not_active Abandoned
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4259843A (en) * | 1979-10-09 | 1981-04-07 | Cromemco Inc. | Isolation chamber for electronic devices |
US4643879A (en) * | 1985-07-01 | 1987-02-17 | American Hospital Supply Corporation | Tower for analyzing system |
US4681741A (en) * | 1985-07-01 | 1987-07-21 | American Hospital Supply Corporation | Reagent dispenser for an analyzing system |
EP0266155A2 (en) * | 1986-10-24 | 1988-05-04 | Richard W. Hanaway | Multiple pipette sampler |
US5000921A (en) * | 1986-10-24 | 1991-03-19 | Hanaway Richard W | Multiple pipette samples |
US5096670A (en) * | 1986-10-31 | 1992-03-17 | Harris Paul C | Automated patient sample analysis instrument |
US5813763A (en) * | 1996-10-11 | 1998-09-29 | Microcal Incorporated | Ultrasensitive differential microcalorimeter |
US6395231B1 (en) * | 1997-10-21 | 2002-05-28 | Cybio Instruments Gmbh | Pipette and handling automatic machine for microtitration plates with permeable bases |
US20030032171A1 (en) * | 2001-08-08 | 2003-02-13 | Dade Behring Inc. | Automated random access microbiological analyzer |
US6573088B2 (en) * | 2001-08-08 | 2003-06-03 | Dade Microscan Inc. | Automated random access microbiological analyzer |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9702793B2 (en) | 2015-03-16 | 2017-07-11 | Todd A Balisky | Variable volume sample capture device |
US20190003995A1 (en) * | 2016-01-14 | 2019-01-03 | Jamlet MONASELIDZE | Differential scanning microcalorimeter device for detecting disease and monitoring therapeutic efficacy |
US11215573B2 (en) * | 2016-01-14 | 2022-01-04 | Jamlet MONASELIDZE | Differential scanning microcalorimeter device for detecting disease and monitoring therapeutic efficacy |
US11946889B2 (en) | 2016-01-14 | 2024-04-02 | Jamlet MONASELIDZE | Differential scanning microcalorimeter device for detecting disease and monitoring therapeutic efficacy |
Also Published As
Publication number | Publication date |
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US20100238968A1 (en) | 2010-09-23 |
WO2009059110A1 (en) | 2009-05-07 |
CN101855541B (en) | 2013-12-11 |
EP2208057A1 (en) | 2010-07-21 |
US8449175B2 (en) | 2013-05-28 |
JP2011503546A (en) | 2011-01-27 |
CN101855541A (en) | 2010-10-06 |
JP5542678B2 (en) | 2014-07-09 |
EP2208057A4 (en) | 2016-06-22 |
US20130171042A1 (en) | 2013-07-04 |
US8827549B2 (en) | 2014-09-09 |
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