US20130125255A1 - Transgenic Plants With Increased Stress Tolerance and Yield - Google Patents

Transgenic Plants With Increased Stress Tolerance and Yield Download PDF

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US20130125255A1
US20130125255A1 US13/666,997 US201213666997A US2013125255A1 US 20130125255 A1 US20130125255 A1 US 20130125255A1 US 201213666997 A US201213666997 A US 201213666997A US 2013125255 A1 US2013125255 A1 US 2013125255A1
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plants
protein
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Amber Shirley
Rodrigo Sarria-Millan
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BASF Plant Science GmbH
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Definitions

  • This invention relates generally to transgenic plants which overexpress nucleic acid sequences encoding polypeptides capable of conferring increased stress tolerance and consequently, increased plant growth and crop yield, under normal or abiotic stress conditions. Additionally, the invention relates to novel isolated nucleic acid sequences encoding polypeptides that confer upon a plant increased tolerance under abiotic stress conditions, and/or increased plant growth and/or increased yield under normal or abiotic stress conditions.
  • Crop yield is defined herein as the number of bushels of relevant agricultural product (such as grain, forage, or seed) harvested per acre. Crop losses and crop yield losses of major crops such as soybean, rice, maize (corn), cotton, and wheat caused by these stresses represent a significant economic and political factor and contribute to food shortages in many underdeveloped countries.
  • WUE has been defined and measured in multiple ways. One approach is to calculate the ratio of whole plant dry weight, to the weight of water consumed by the plant throughout its life. Another variation is to use a shorter time interval when biomass accumulation and water use are measured. Yet another approach is to use measurements from restricted parts of the plant, for example, measuring only aerial growth and water use. WUE also has been defined as the ratio of CO 2 uptake to water vapor loss from a leaf or portion of a leaf, often measured over a very short time period (e.g. seconds/minutes). The ratio of 13 C/ 12 C fixed in plant tissue, and measured with an isotope ratio mass-spectrometer, also has been used to estimate WUE in plants using C 3 photosynthesis.
  • An increase in WUE is informative about the relatively improved efficiency of growth and water consumption, but this information taken alone does not indicate whether one of these two processes has changed or both have changed.
  • an increase in WUE due to a decrease in water use, without a change in growth would have particular merit in an irrigated agricultural system where the water input costs were high.
  • An increase in WUE driven mainly by an increase in growth without a corresponding jump in water use would have applicability to all agricultural systems.
  • an increase in growth even if it came at the expense of an increase in water use (i.e. no change in WUE), could also increase yield. Therefore, new methods to increase both WUE and biomass accumulation are required to improve agricultural productivity.
  • Concomitant with measurements of parameters that correlate with abiotic stress tolerance are measurements of parameters that indicate the potential impact of a transgene on crop yield.
  • the plant biomass correlates with the total yield.
  • other parameters have been used to estimate yield, such as plant size, as measured by total plant dry weight, above-ground dry weight, above-ground fresh weight, leaf area, stem volume, plant height, rosette diameter, leaf length, root length, root mass, tiller number, and leaf number.
  • Plant size at an early developmental stage will typically correlate with plant size later in development. A larger plant with a greater leaf area can typically absorb more light and carbon dioxide than a smaller plant and therefore will likely gain a greater weight during the same period.
  • Harvest index the ratio of seed yield to above-ground dry weight, is relatively stable under many environmental conditions and so a robust correlation between plant size and grain yield is possible.
  • Plant size and grain yield are intrinsically linked, because the majority of grain biomass is dependent on current or stored photosynthetic productivity by the leaves and stem of the plant. Therefore, selecting for plant size, even at early stages of development, has been used as to screen for plants that may demonstrate increased yield when exposed to field testing.
  • measurements of plant size in early development, under standardized conditions in a growth chamber or greenhouse are standard practices to measure potential yield advantages conferred by the presence of a transgene.
  • Newly generated stress tolerant plants and/or plants with increased water use efficiency will have many advantages, such as an increased range in which the crop plants can be cultivated, by for example, decreasing the water requirements of a plant species.
  • Other desirable advantages include increased resistance to lodging, the bending of shoots or stems in response to wind, rain, pests, or disease.
  • the present inventors have discovered that transforming a plant with certain polynucleotides results in enhancement of the plant's growth and response to environmental stress, and accordingly the yield of the agricultural products of the plant is increased, when the polynucleotides are present in the plant as transgenes.
  • the polynucleotides capable of mediating such enhancements have been isolated from Arabidopsis thaliana, Capsicum annuum, Escherichia coil, Physcomitrella patens, Saccharomyces cerevisiae, Triticum aestivum, Zea mays, Glycine max, Linum usitatissimum, Triticum aestivum, Oryza sativa, Helianthus annuus , and Brassica napus and the sequences thereof are set forth in the Sequence Listing as indicated in Table 1.
  • ZM62051019 Z. mays 29 30 ZM65086957 Z. mays 31 32 ZM68587657 Z. mays 33 34 EST390 P. patens 35 36 BN51363030 B. napus 37 38 BN42986056 B. napus 39 40 BN49389066 B. napus 41 42 BN51339479 B. napus 43 44 ZM57651070 Z. mays 45 46 ZM62073276 Z. mays 47 48 EST257 P. patens 49 50 LU61665952 L. usitatissimum 51 52 TA56863186 T. aestivum 53 54 ZM62026837 Z. mays 55 56 ZM65457595 Z.
  • ⁇ 58 ZM67230154 Z. mays 59 60 EST465 P. patens 61 62 YBL109w S. cerevisiae 63 64 YBL100c S. cerevisiae 65 66 YKL184w S. cerevisiae 67 68 YPL091w S. cerevisiae 69 70 TA54587433 T. aestivum 71 72 ZM68532504 Z. mays 73 74 BN42856089 B. napus 75 76 BN43206527 B. napus 77 78 HA66872964 H. annuus 79 80 LU61662612 L. usitatissimum 81 82 OS32806943 O.
  • sativa 83 84 OS34738749 O. sativa 85
  • 86 ZM59400933 Z. mays 87 88 ZM62132060 Z. mays 89 90 ZM59202533 Z. mays 91 92 BN41901422 B. napus 93 94 BN47868329 B. napus 95 96 BN42671700 B. napus 97 98 ZM68416988 Z. mays 99 100
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a CAAX amino terminal protease family protein.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a prenyl-dependent CAAX protease.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a SAR8.2 protein precursor.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a putative membrane protein.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein phosphatase 2C protein.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a mitochondrial carrier protein.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein kinase.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a peptidyl prolyl isomerase.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a unknown protein 1.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an Isolated polynucleotide encoding a unknown protein 2.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a ornithine decarboxylase.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a glutathione reductase.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a unknown protein 3.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein phosphatase 2A protein.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a MEK1 protein kinase.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a AP2 domain containing transcription factor.
  • the invention concerns a seed produced by the transgenic plant of the invention, wherein the seed is true breeding for a transgene comprising the polynucleotide described above.
  • Plants derived from the seed of the invention demonstrate increased tolerance to an environmental stress, and/or increased plant growth, and/or increased yield, under normal or stress conditions as compared to a wild type variety of the plant.
  • the invention concerns products produced by or from the transgenic plants of the invention, their plant parts, or their seeds, such as a foodstuff, feedstuff, food supplement, feed supplement, cosmetic or pharmaceutical.
  • the invention further provides certain isolated polynucleotides identified in Table 1, and certain isolated polypeptides identified in Table 1.
  • the invention is also embodied in recombinant vector comprising an isolated polynucleotide of the invention.
  • the invention concerns a method of producing the aforesaid transgenic plant, wherein the method comprises transforming a plant cell with an expression vector comprising an isolated polynucleotide of the invention, and generating from the plant cell a transgenic plant that expresses the polypeptide encoded by the polynucleotide. Expression of the polypeptide in the plant results in increased tolerance to an environmental stress, and/or growth, and/or yield under normal and/or stress conditions as compared to a wild type variety of the plant.
  • the invention provides a method of increasing a plant's tolerance to an environmental stress, and/or growth, and/or yield.
  • the method comprises the steps of transforming a plant cell with an expression cassette comprising an isolated polynucleotide of the invention, and generating a transgenic plant from the plant cell, wherein the transgenic plant comprises the polynucleotide.
  • FIG. 1 shows an alignment of the disclosed amino acid sequences AtFACE-2 (SEQ ID NO:6), ZM57353913 (SEQ ID NO:8), and ZM59252659 (SEQ ID NO:10).
  • the alignment was generated using Align X of Vector NTI.
  • FIG. 2 shows an alignment of the disclosed amino acid sequences EST564 (SEQ ID NO:16), BN49502266 (SEQ ID NO:18), GM49788080 (SEQ ID NO:20), GM53049821 (SEQ ID NO:22), ZM58462719 (SEQ ID NO:24), ZM61092633 (SEQ ID NO:26), ZM62016485 (SEQ ID NO:28), ZM62051019 (SEQ ID NO:30), ZM65086957 (SEQ ID NO:32), and ZM68587657 (SEQ ID NO:34).
  • the alignment was generated using Align X of Vector NTI.
  • FIG. 3 shows an alignment of the disclosed amino acid sequences EST390 (SEQ ID NO:36), BN51363030 (SEQ ID NO:38), BN42986056 (SEQ ID NO:40), BN49389066 (SEQ ID NO:42), BN51339479 (SEQ ID NO:44), ZM57651070 (SEQ ID NO:46), and ZM62073276 (SEQ ID NO:48).
  • the alignment was generated using Align X of Vector NTI.
  • FIG. 4 shows an alignment of the disclosed amino acid sequences EST257 (SEQ ID NO:50), LU61665952 (SEQ ID NO:52), TA56863186 (SEQ ID NO:54), ZM62026837 (SEQ ID NO:56), ZM65457595 (SEQ ID NO:58), ZM67230154 (SEQ ID NO:60).
  • the alignment was generated using Align X of Vector NTI.
  • FIG. 5 shows an alignment of the disclosed amino acid sequences ZM68532504 (SEQ ID NO:74), BN42856089 (SEQ ID NO:76), BN43206527 (SEQ ID NO:78), HA66872964 (SEQ ID NO:80), LU61662612 (SEQ ID NO:82), 0832806943 (SEQ ID NO:84), OS34738749 (SEQ ID NO:86), ZM59400933 (SEQ ID NO:88), and ZM62132060 (SEQ ID NO:90).
  • the alignment was generated using Align X of Vector NTI.
  • FIG. 6 shows an alignment of the disclosed amino acid sequences ZM59202533 (SEQ ID NO:92), 6N41901422 (SEQ ID NO:94), BN47868329 (SEQ ID NO:96), and ZM68416988 (SEQ ID NO:100).
  • the alignment was generated using Align X of Vector NTI.
  • the invention provides a transgenic plant that overexpresses an isolated polynucleotide identified in Table 1, or a homolog thereof.
  • the transgenic plant of the invention demonstrates an increased tolerance to an environmental stress as compared to a wild type variety of the plant.
  • the overexpression of such isolated nucleic acids in the plant may optionally result in an increase in plant growth or in yield of associated agricultural products, under normal or stress conditions, as compared to a wild type variety of the plant.
  • a “transgenic plant” is a plant that has been altered using recombinant DNA technology to contain an isolated nucleic acid which would otherwise not be present in the plant.
  • the term “plant” includes a whole plant, plant cells, and plant parts. Plant parts include, but are not limited to, stems, roots, ovules, stamens, leaves, embryos, meristematic regions, callus tissue, gametophytes, sporophytes, pollen, microspores, and the like.
  • the transgenic plant of the invention may be male sterile or male fertile, and may further include transgenes other than those that comprise the isolated polynucleotides described herein.
  • the term “variety” refers to a group of plants within a species that share constant characteristics that separate them from the typical form and from other possible varieties within that species. While possessing at least one distinctive trait, a variety is also characterized by some variation between individuals within the variety, based primarily on the Mendelian segregation of traits among the progeny of succeeding generations. A variety is considered “true breeding” for a particular trait if it is genetically homozygous for that trait to the extent that, when the true-breeding variety is self-pollinated, a significant amount of independent segregation of the trait among the progeny is not observed.
  • the trait arises from the transgenic expression of one or more isolated polynucleotides introduced into a plant variety.
  • wild type variety refers to a group of plants that are analyzed for comparative purposes as a control plant, wherein the wild type variety plant is identical to the transgenic plant (plant transformed with an isolated polynucleotide in accordance with the invention) with the exception that the wild type variety plant has not been transformed with an isolated polynucleotide of the invention.
  • nucleic acid and “polynucleotide” are interchangeable and refer to RNA or DNA that is linear or branched, single or double stranded, or a hybrid thereof. The term also encompasses RNA/DNA hybrids.
  • An “isolated” nucleic acid molecule is one that is substantially separated from other nucleic acid molecules which are present in the natural source of the nucleic acid (i.e., sequences encoding other polypeptides). For example, a cloned nucleic acid is considered isolated.
  • a nucleic acid is also considered isolated if it has been altered by human intervention, or placed in a locus or location that is not its natural site, or if it is introduced into a cell by transformation.
  • an isolated nucleic acid molecule such as a cDNA molecule
  • the term “environmental stress” refers to a sub-optimal condition associated with salinity, drought, nitrogen, temperature, metal, chemical, pathogenic, or oxidative stresses, or any combination thereof.
  • water use efficiency and “WUE” refer to the amount of organic matter produced by a plant divided by the amount of water used by the plant in producing it, i.e., the dry weight of a plant in relation to the plant's water use.
  • dry weight refers to everything in the plant other than water, and includes, for example, carbohydrates, proteins, oils, and mineral nutrients.
  • transgenic plant of the invention may be a dicotyledonous plant or a monocotyledonous plant.
  • transgenic plants of the invention may be derived from any of the following dicotyledonous plant families: Leguminosae, including plants such as pea, alfalfa and soybean; Umbelliferae, including plants such as carrot and celery; Solanaceae, including the plants such as tomato, potato, aubergine, tobacco, and pepper; Cruciferae, particularly the genus Brassica , which includes plant such as oilseed rape, beet, cabbage, cauliflower and broccoli); and A.
  • Transgenic plants of the invention may be derived from monocotyledonous plants, such as, for example, wheat, barley, sorghum, millet, rye, triticale, maize, rice, oats and sugarcane.
  • Transgenic plants of the invention are also embodied as trees such as apple, pear, quince, plum, cherry, peach, nectarine, apricot, papaya, mango, and other woody species including coniferous and deciduous trees such as poplar, pine, sequoia, cedar, oak, and the like.
  • Arabidopsis thaliana are also embodied as trees such as apple, pear, quince, plum, cherry, peach, nectarine, apricot, papaya, mango, and other woody species including coniferous and deciduous trees such as poplar, pine, sequoia, cedar, oak, and the like.
  • Arabidopsis thaliana are also embodied as trees such as
  • one embodiment of the invention is a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a CAAX amino terminal protease family protein.
  • the transgenic plant of this embodiment may comprise any polynucleotide encoding a CAAX amino terminal protease family protein.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a CAAX amino terminal protease family protein having a sequence comprising amino acids 1 to 301 of SEQ ID NO:2; and a protein having a sequence comprising amino acids 1 to 293 of SEQ ID NO:4.
  • the Invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a prenyl-dependent CAAX protease.
  • the transgenic'plant of this embodiment may comprise any polynucleotide encoding a prenyl-dependent CAAX protease.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a prenyl-dependent CAAX protease having a sequence comprising amino acids 1 to 311 of SEQ ID NO:6; a protein having a sequence comprising amino acids 1 to 313 of SEQ ID NO:8; a protein having a sequence comprising amino acids 1 to 269 of SEQ ID NO:10.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a SAR8.2 protein precursor.
  • the transgenic plant of this embodiment may comprise any polynucleotide encoding a SAR8.2 protein precursor.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a SAR8.2 protein precursor having a sequence comprising amino acids 1 to 86 of SEQ ID NO:12.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a putative membrane protein.
  • the transgenic plant of this embodiment may comprise any polynucleotide encoding a putative membrane protein.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a putative membrane protein having a sequence comprising amino acids 1 to 696 of SEQ ID NO:14.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein phosphatase 2C protein.
  • the transgenic plant of this embodiment may comprise any polynucleotide encoding a protein phosphatase 2C protein.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a protein phosphatase 2C protein having a sequence comprising amino acids 1 to 284 of SEQ ID NO:16; a protein having a sequence comprising amino acids 1 to 384 of SEQ ID NO:18; a protein having a sequence comprising amino acids 1 to 346 of SEQ ID NO:20; a protein having a sequence comprising amino acids 1 to 375 of SEQ ID NO:22; a protein having a sequence comprising amino acids 1 to 390 of SEQ ID NO:24; a protein having a sequence comprising amino acids 1 to 398 of SEQ ID NO:26; a protein having a sequence comprising amino acids 1 to 399 of SEQ ID NO:28; a protein having a sequence comprising amino acids 1 to 399 of SEQ ID NO:30; a protein having a sequence comprising amino acids 1 to 399 of SEQ ID NO:32; a protein having a sequence comprising amino acids 1 to 276 of SEQ ID
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a mitochondrial carrier protein.
  • the transgenic plant of this embodiment may comprise any polynucleotide encoding a mitochondrial carrier protein.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a mitochondrial carrier protein having a sequence comprising amino acids 1 to 303 of SEQ ID NO:36; a protein having a sequence comprising amino acids 1 to 315 of SEQ ID NO:38; a protein having a sequence comprising amino acids 1 to 289 of SEQ ID NO:40; a protein having a sequence comprising amino acids 1 to 303 of SEQ ID NO:42; a protein having a sequence comprising amino acids 1 to 299 of SEQ ID NO:44; a protein having a sequence comprising amino acids 1 to 299 of SEQ ID NO:46; a protein having a sequence comprising amino acids 1 to 311 of SEQ ID NO:48.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein kinase.
  • the transgenic plant of this embodiment may comprise any polynucleotide encoding a protein kinase.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a protein kinase having a sequence comprising amino acids 1 to 356 of SEQ ID NO:50; a protein having a sequence comprising amino acids 1 to 364 of SEQ ID NO:52; a protein having a sequence comprising amino acids 1 to 361 of SEQ ID NO:54; a protein having a sequence comprising amino acids 1 to 370 of SEQ ID NO:56; a protein having a sequence comprising amino acids 1 to 377 of SEQ ID NO:58; a protein having a sequence comprising amino acids 1 to 382 of SEQ ID NO:60.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a peptidyl prolyl isomerase.
  • the transgenic plant of this embodiment may comprise any polynucleotide encoding a peptidyl prolyl isomerase.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a peptidyl prolyl isomerase having a sequence comprising amino acids 1 to 523 of SEQ ID NO:62.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding an unknown protein 1.
  • the transgenic plant of this embodiment may comprise any polynucleotide encoding an unknown protein 1.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a unknown protein 1 having a sequence comprising amino acids 1 to 111 of SEQ ID NO:64.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding an unknown protein 2.
  • the transgenic plant of this embodiment may comprise any polynucleotide encoding an unknown protein 2.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a unknown protein 2 having a sequence comprising amino acids 1 to 104 of SEQ ID NO:66.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a ornithine decarboxylase.
  • the transgenic plant of this embodiment may comprise any polynucleotide encoding a ornithine decarboxylase.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a ornithine decarboxylase having a sequence comprising amino acids 1 to 466 of SEQ ID NO:68.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a glutathione reductase.
  • the transgenic plant of this embodiment may comprise any polynucleotide encoding a glutathione reductase.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a glutathione reductase having a sequence comprising amino acids 1 to 483 of SEQ ID NO:70.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding an unknown protein 3.
  • the transgenic plant of this embodiment may comprise any polynucleotide encoding a unknown protein 3.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a unknown protein 3 having a sequence comprising amino acids 1 to 129 of SEQ ID NO:72.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein phosphatase 2A protein.
  • the transgenic plant of this embodiment may comprise any polynucleotide encoding a protein phosphatase 2A protein.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a protein phosphatase 2A protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:74; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:76; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:78; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:80; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:82; a protein having a sequence comprising amino acids 1 to 307 of SEQ ID NO:84; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:86; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:88; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:90.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a MEK1 protein kinase.
  • the transgenic plant of this embodiment may comprise any polynucleotide encoding a MEK1 protein kinase.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a MEK1 protein kinase having a sequence comprising amino acids 1 to 355 of SEQ ID NO:92; a protein having a sequence comprising amino acids 1 to 355 of SEQ ID NO:94; a protein having a sequence comprising amino acids 1 to 338 of SEQ ID NO:96; a protein having a sequence comprising amino acids 1 to 350 of SEQ ID NO:100.
  • the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding an AP2 domain containing transcription factor.
  • the transgenic plant of this embodiment may comprise any polynucleotide encoding a AP2 domain containing transcription factor.
  • the transgenic plant of this embodiment comprises a polynucleotide encoding a AP2 domain containing transcription factor having a sequence comprising amino acids 1 to 197 of SEQ ID NO:98.
  • the invention further provides a seed produced by a transgenic plant expressing polynucleotide listed in Table 1, wherein the seed contains the polynucleotide, and wherein the plant is true breeding for increased growth and/or yield under normal or stress conditions and/or increased tolerance to an environmental stress as compared to a wild type variety of the plant.
  • the invention also provides a product produced by or from the transgenic plants expressing the polynucleotide, their plant parts, or their seeds.
  • the product can be obtained using various methods well known in the art.
  • the word “product” includes, but not limited to, a foodstuff, feedstuff, a food supplement, feed supplement, cosmetic or pharmaceutical. Foodstuffs are regarded as compositions used for nutrition or for supplementing nutrition.
  • Animal feedstuffs and animal feed supplements are regarded as foodstuffs.
  • the invention further provides an agricultural product produced by any of the transgenic plants, plant parts, and plant seeds.
  • Agricultural products include, but are not limited to, plant extracts, proteins, amino acids, carbohydrates, fats, oils, polymers, vitamins, and the like.
  • an isolated polynucleotide of the invention comprises a polynucleotide having a sequence selected from the group consisting of the polynucleotide sequences listed in Table 1. These polynucleotides may comprise sequences of the coding region, as well as 5′ untranslated sequences and 3′ untranslated sequences.
  • a polynucleotide of the invention can be isolated using standard molecular biology techniques and the sequence information provided herein, for example, using an automated DNA synthesizer.
  • “Homologs” are defined herein as two nucleic acids or polypeptides that have similar, or substantially identical, nucleotide or amino acid sequences, respectively. Homologs include allelic variants, analogs, and orthologs, as defined below. As used herein, the term “analogs” refers to two nucleic acids that have the same or similar function, but that have evolved separately in unrelated organisms. As used herein, the term “orthologs” refers to two nucleic acids from different species, but that have evolved from a common ancestral gene by speciation.
  • homolog further encompasses nucleic acid molecules that differ from one of the nucleotide sequences shown in Table 1 due to degeneracy of the genetic code and thus encode the same polypeptide.
  • a “naturally occurring” nucleic acid molecule refers to an RNA or DNA molecule having a nucleotide sequence that occurs in nature (e.g., encodes a natural polypeptide).
  • sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in the sequence of one polypeptide for optimal alignment with the other polypeptide or nucleic acid).
  • amino acid residues at corresponding amino acid positions are then compared. When a position in one sequence is occupied by the same amino acid residue as the corresponding position in the other sequence then the molecules are identical at that position. The same type of comparison can be made between two nucleic acid sequences.
  • the isolated amino acid homologs, analogs, and orthologs of the polypeptides of the present invention are at least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-75%, 75-80%, 80-85%, 85-90%, or 90-95%, and most preferably at least about 96%, 97%, 98%, 99%, or more identical to an entire amino acid sequence identified in Table 1.
  • an isolated nucleic acid homolog of the invention comprises a nucleotide sequence which is at least about 40-60%, preferably at least about 60-70%, more preferably at least about 70-75%, 75-80%, 80-85%, 85-90%, or 90-95%, and even more preferably at least about 95%, 96%, 97%, 98%, 99%, or more identical to a nucleotide sequence shown in Table 1.
  • the percent sequence identity between two nucleic acid or polypeptide sequences is determined using the Vector NTI 9.0 (PC) software package (Invitrogen, 1600 Faraday Ave., Carlsbad, Calif. 92008).
  • a gap opening penalty of 15 and a gap extension penalty of 6.66 are used for determining the percent identity of two nucleic acids.
  • a gap opening penalty of 10 and a gap extension penalty of 0.1 are used for determining the percent identity of two polypeptides. All other parameters are set at the default settings.
  • the gap opening penalty is 10
  • the gap extension penalty is 0.05 with blosum62 matrix. It is to be understood that for the purposes of determining sequence identity when comparing a DNA sequence to an RNA sequence, a thymidine nucleotide is equivalent to a uracil nucleotide.
  • Nucleic acid molecules corresponding to homologs, analogs, and orthologs of the polypeptides listed in Table 1 can be isolated based on their Identity to said polypeptides, using the polynucleotides encoding the respective polypeptides or primers based thereon, as hybridization probes according to standard hybridization techniques under stringent hybridization conditions.
  • stringent conditions refers to hybridization overnight at 60° C. in 10 ⁇ Denhart's solution, 6 ⁇ SSC, 0.5% SDS, and 100 ⁇ g/ml denatured salmon sperm DNA. Blots are washed sequentially at 62° C.
  • stringent conditions refers to hybridization in a 6 ⁇ SSC solution at 65° C.
  • highly stringent conditions refers to hybridization overnight at 65° C. in 10 ⁇ Denhart's solution, 6 ⁇ SSC, 0.5% SDS and 100 ⁇ g/ml denatured salmon sperm DNA. Blots are washed sequentially at 65° C. for 30 minutes each time in 3 ⁇ SSC/0.1% SDS, followed by 1 ⁇ SSC/0.1% SDS, and finally 0.1 ⁇ SSC/0.1% SDS.
  • nucleic acid hybridizations are well known in the art.
  • an isolated nucleic acid molecule of the invention that hybridizes under stringent or highly stringent conditions to a nucleotide sequence listed in Table 1 corresponds to a naturally occurring nucleic acid molecule.
  • an optimized nucleic acid encodes a polypeptide that has a function similar to those of the polypeptides listed in Table 1 and/or modulates a plant's growth and/or yield under normal and/or water-limited conditions and/or tolerance to an environmental stress, and more preferably increases a plant's growth and/or yield under normal and/or water-limited conditions and/or tolerance to an environmental stress upon its overexpression in the plant.
  • “optimized” refers to a nucleic acid that is genetically engineered to increase its expression in a given plant or animal.
  • the DNA sequence of the gene can be modified to: 1) comprise codons preferred by highly expressed plant genes; 2) comprise an A+T content in nucleotide base composition to that substantially found in plants; 3) form a plant initiation sequence; 4) to eliminate sequences that cause destabilization, inappropriate polyadenylation, degradation and termination of RNA, or that form secondary structure hairpins or RNA splice sites; or 5) elimination of antisense open reading frames.
  • Increased expression of nucleic acids in plants can be achieved by utilizing the distribution frequency of codon usage in plants in general or in a particular plant. Methods for optimizing nucleic acid expression in plants can be found in EPA 0359472; EPA 0385962; PCT Application No.
  • An isolated polynucleotide of the invention can be optimized such that its distribution frequency of codon usage deviates, preferably, no more than 25% from that of highly expressed plant genes and, more preferably, no more than about 10%.
  • the XCG (where X is A, T, C, or G) nucleotide is the least preferred codon in dicots, whereas the XTA codon is avoided in both monocots and dicots.
  • Optimized nucleic acids of this invention also preferably have CG and TA doublet avoidance indices closely approximating those of the chosen host plant. More preferably, these indices deviate from that of the host by no more than about 10-15%.
  • the invention further provides an isolated recombinant expression vector comprising a polynucleotide as described above, wherein expression of the vector in a host cell results in the plant's increased growth and/or yield under normal or water-limited conditions and/or increased tolerance to environmental stress as compared to a wild type variety of the host cell.
  • the recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression, which is operatively linked to the nucleic acid sequence to be expressed.
  • operatively linked is intended to mean that the nucleotide sequence of interest is linked to the regulatory sequence(s) in a manner which allows for expression of the nucleotide sequence (e.g., in a bacterial or plant host cell when the vector is introduced into the host cell).
  • regulatory sequence is intended to include promoters, enhancers, and other expression control elements (e.g., polyadenylation signals). Such regulatory sequences are well known in the art. Regulatory sequences include those that direct constitutive expression of a nucleotide sequence in many types of host cells and those that direct expression of the nucleotide sequence only in certain host cells or under certain conditions.
  • the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of polypeptide desired, etc.
  • the expression vectors of the invention can be introduced into host cells to thereby produce polypeptides encoded by nucleic acids as described herein.
  • Plant gene expression should be operatively linked to an appropriate promoter conferring gene expression in a timely, cell specific, or tissue specific manner.
  • Promoters useful in the expression cassettes of the invention include any promoter that is capable of initiating transcription in a plant cell. Such promoters include, but are not limited to, those that can be obtained from plants, plant viruses, and bacteria that contain genes that are expressed in plants, such as Agrobacterium and Rhizobium.
  • the promoter may be constitutive, inducible, developmental stage-preferred, cell type-preferred, tissue-preferred, or organ-preferred. Constitutive promoters are active under most conditions. Examples of constitutive promoters include the CaMV 19S and 35S promoters, the sX CaMV 355 promoter, the Sep1 promoter, the rice actin promoter, the Arabidopsis actin promoter, the ubiquitan promoter, pEmu, the figwort mosaic virus 35S promoter, the Smas promoter, the super promoter (U.S. Pat. No. 5,955,646), the GRP1-8 promoter, the cinnamyl alcohol dehydrogenase promoter (U.S. Pat. No.
  • promoters from the T-DNA of Agrobacterium such as mannopine synthase, nopaline synthase, and octopine synthase, the small subunit of ribulose biphosphate carboxylase (ssu-RUBISCO) promoter, and the like.
  • Inducible promoters are preferentially active under certain environmental conditions, such as the presence or absence of a nutrient or metabolite, heat or cold, light, pathogen attack, anaerobic conditions, and the like.
  • the hsp80 promoter from Brassica is induced by heat shock
  • the PPDK promoter is induced by light
  • the PR-1 promoters from tobacco, Arabidopsis , and maize are inducible by infection with a pathogen
  • the Adh1 promoter is Induced by hypoxia and cold stress.
  • Plant gene expression can also be facilitated via an inducible promoter (For a review, see Gatz, 1997, Annu. Rev. Plant Physiol. Plant Mol. Biol. 48:89-108).
  • Chemically inducible promoters are especially suitable if gene expression is wanted to occur in a time specific manner.
  • Examples of such promoters are a salicylic acid inducible promoter (PCT Application No. WO 95/19443), a tetracycline inducible promoter (Gatz et al., 1992, Plant J. 2: 397-404), and an ethanol inducible promoter (PCT Application No. WO 93/21334).
  • the inducible promoter is a stress-inducible promoter.
  • stress-inducible promoters are preferentially active under one or more of the following stresses: sub-optimal conditions associated with salinity, drought, nitrogen, temperature, metal, chemical, pathogenic, and oxidative stresses.
  • Stress inducible promoters include, but are not limited to, Cor78 (Chak et al., 2000, Planta 210:875-883; Hovath et al., 1993, Plant Physiol.
  • tissue and organ preferred promoters include those that are preferentially expressed in certain tissues or organs, such as leaves, roots, seeds, or xylem.
  • tissue-preferred and organ-preferred promoters include, but are not limited to fruit-preferred, ovule-preferred, male tissue-preferred, seed-preferred, integument-preferred, tuber-preferred, stalk-preferred, pericarp-preferred, leaf-preferred, stigma-preferred, pollen-preferred, anther-preferred, petal-preferred, sepal-preferred, pedicel-preferred, silique-preferred, stem-preferred, root-preferred promoters, and the like.
  • Seed-preferred promoters are preferentially expressed during seed development and/or germination.
  • seed-preferred promoters can be embryo-preferred, endosperm-preferred, and seed coat-preferred (See Thompson at al., 1989, BioEssays 10:108).
  • seed-preferred promoters include, but are not limited to, cellulose synthase (celA), Cim1, gamma-zein, globulin-1, maize 19 kD zein (cZ19B1), and the like.
  • tissue-preferred or organ-preferred promoters include the napin-gene promoter from rapeseed (U.S. Pat. No. 5,608,152), the USP-promoter from Vicia faba (Baeumlein et al., 1991, Mol. Gen. Genet. 225(3): 459-67), the oleosin-promoter from Arabidopsis (PCT Application No. WO 98/45461), the phaseolin-promoter from Phaseolus vulgaris (U.S. Pat. No. 5,504,200), the Bce4-promoter from Brassica (PCT Application No.
  • WO 91/13980 or the legumin B4 promoter (LeB4; Baeumlein et al., 1992, Plant Journal, 2(2): 233-9), as well as promoters conferring seed specific expression in monocot plants like maize, barley, wheat, rye, rice, etc.
  • Suitable promoters to note are the lpt2 or lpt1-gene promoter from barley (PCT Application No. WO 95/15389 and PCT Application No. WO 95/23230) or those described in PCT Application No.
  • WO 99/16890 promoters from the barley hordein-gene, rice glutelin gene, rice oryzin gene, rice prolamin gene, wheat gliadin gene, wheat glutelin gene, oat glutelin gene, Sorghum kasirin-gene, and rye secalin gene).
  • promoters useful in the expression cassettes of the invention include, but are not limited to, the major chlorophyll a/b binding protein promoter, histone promoters, the Ap3 promoter, the ⁇ -conglycin promoter, the napin promoter, the soybean lectin promoter, the maize 15 kD zein promoter, the 22 kD zein promoter, the 27 kD zein promoter, the g-zein promoter, the waxy, shrunken 1, shrunken 2, and bronze promoters, the Zm13 promoter (U.S. Pat. No. 5,086,169), the maize polygalacturonase promoters (PG) (U.S. Pat. Nos. 5,412,085 and 5,545,546), and the SGB6 promoter (U.S. Pat. No. 5,470,359), as well as synthetic or other natural promoters.
  • the major chlorophyll a/b binding protein promoter include, but are not limited to, the major chlor
  • Additional flexibility in controlling heterologous gene expression in plants may be obtained by using DNA binding domains and response elements from heterologous sources (i.e., DNA binding domains from non-plant sources).
  • heterologous DNA binding domain is the LexA DNA binding domain (Brent and Ptashne, 1985, Cell 43:729-736).
  • the polynucleotides listed in Table 1 are expressed in plant cells from higher plants (e.g., the spermatophytes, such as crop plants).
  • a polynucleotide may be “introduced” into a plant cell by any means, including transfection, transformation or transduction, electroporation, particle bombardment, agroinfection, and the like. Suitable methods for transforming or transfecting plant cells are disclosed, for example, using particle bombardment as set forth in U.S. Pat. Nos. 4,945,050; 5,036,006; 5,100,792; 5,302,523; 5,464,765; 5,120,657; 6,084,154; and the like.
  • the transgenic corn seed of the invention may be made using Agrobacterium transformation, as described in U.S. Pat. Nos. 5,591,616; 5,731,179; 5,981,840; 5,990,387; 6,162,965; 6,420,630, U.S. patent application publication number 2002/0104132, and the like. Transformation of soybean can be performed using for example a technique described in European Patent No. EP 0424047, U.S. Pat. No. 5,322,783, European Patent No. EP 0397 687, U.S. Pat. No. 5,376,543, or U.S. Pat. No. 5,169,770. A specific example of wheat transformation can be found in PCT Application No. WO 93/07256.
  • Cotton may be transformed using methods disclosed in U.S. Pat. Nos. 5,004,863; 5,159,135; 5,846,797, and the like. Rice may be transformed using methods disclosed in U.S. Pat. Nos. 4,666,844; 5,350,688; 6,153,813; 6,333,449; 6,288,312; 6,365,807; 6,329,571, and the like. Other plant transformation methods are disclosed, for example, in U.S. Pat. Nos. 5,932,782; 6,153,811; 6,140,553; 5,969,213; 6,020,539, and the like. Any plant transformation method suitable for inserting a transgene into a particular plant may be used in accordance with the invention.
  • the introduced polynucleotide may be maintained in the plant cell stably if it is incorporated into a non-chromosomal autonomous replicon or integrated into the plant chromosomes.
  • the introduced polynucleotide may be present on an extra-chromosomal non-replicating vector and may be transiently expressed or transiently active.
  • Another aspect of the invention pertains to an isolated polypeptide having a sequence selected from the group consisting of the polypeptide sequences listed in Table 1.
  • An “isolated” or “purified” polypeptide is free of some of the cellular material when produced by recombinant DNA techniques, or chemical precursors or other chemicals when chemically synthesized.
  • the language “substantially free of cellular material” includes preparations of a polypeptide in which the polypeptide is separated from some of the cellular components of the cells in which it is naturally or recombinantly produced.
  • the language “substantially free of cellular material” includes preparations of a polypeptide of the invention having less than about 30% (by dry weight) of contaminating polypeptides, more preferably less than about 20% of contaminating polypeptides, still more preferably less than about 10% of contaminating polypeptides, and most preferably less than about 5% contaminating polypeptides.
  • the invention is also embodied in a method of producing a transgenic plant comprising at least one polynucleotide listed in Table 1, wherein expression of the polynucleotide in the plant results in the plant's increased growth and/or yield under normal or water-limited conditions and/or increased tolerance to an environmental stress as compared to a wild type variety of the plant comprising the steps of: (a) introducing into a plant cell an expression vector comprising at least one polynucleotide listed in Table 1, and (b) generating from the plant cell a transgenic plant that expresses the polynucleotide, wherein expression of the polynucleotide in the transgenic plant results in the plant's increased growth and/or yield under normal or water-limited conditions and/or increased tolerance to environmental stress as compared to a wild type variety of the plant.
  • the plant cell may be, but is not limited to, a protoplast, gamete producing cell, and a cell that regenerates into a whole plant.
  • transgenic refers to any plant, plant cell, callus, plant tissue, or plant part, that contains at least one recombinant polynucleotide listed in Table 1.
  • the recombinant polynucleotide is stably integrated into a chromosome or stable extra-chromosomal element, so that it is passed on to successive generations.
  • the present invention also provides a method of increasing a plant's growth and/or yield under normal or water-limited conditions and/or increasing a plant's tolerance to an environmental stress comprising the steps of increasing the expression of at least one polynucleotide listed in Table 1 in the plant.
  • Expression of a protein can be increased by any method known to those of skill in the art.
  • the effect of the genetic modification on plant growth and/or yield and/or stress tolerance can be assessed by growing the modified plant under normal and.or less than suitable conditions and then analyzing the growth characteristics and/or metabolism of the plant.
  • analysis techniques are well known to one skilled in the art, and include dry weight, wet weight, polypeptide synthesis, carbohydrate synthesis, lipid synthesis, evapotranspiration rates, general plant and/or crop yield, flowering, reproduction, seed setting, root growth, respiration rates, photosynthesis rates, metabolite composition, etc., using methods known to those of skill in biotechnology.
  • cDNAs were isolated from proprietary libraries of the respective plant species using known methods. Sequences were processed and annotated using bioinformatics analyses. The degrees of amino acid identity and similarity of the isolated sequences to the respective closest known public sequences are indicated in Tables 2 through 18 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).
  • AtFACE-2 SEQ ID NO: 6
  • NP_850262 A. thaliana 100.00% BAC43705
  • thaliana 99.70% CAN61196 Vitis vinifera 36.70%
  • XP_695285 Danio rerio 32.70%
  • XP_001342272 D. rerio 32.70%
  • the full-length DNA sequence of the AtFACE-2 (SEQ ID NO: 5) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e ⁇ 10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Two homologs from maize were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 19 and 20 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix; blosum62).
  • the full-length DNA sequence of EST564 (SEQ ID NO: 15) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e ⁇ 10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Six homologs from maize, two homologs from soybean, and one homolog from canda were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 21-29 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).
  • the full-length DNA sequence of the EST390 (SEQ ID NO: 35) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e ⁇ 10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Four homologs from canola and two homologs from maize were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 30-35 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum82).
  • the full-length DNA sequence of the EST257 (SEQ ID NO: 49) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e ⁇ 10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Three homologs from maize, one homolog from linseed, and one sequence from wheat were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 36-40 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).
  • the full-length DNA sequence of the ZM68532504 (SEQ ID NO: 73) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e ⁇ 10 (Altschul at al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Two homologs from canola, two homologs from maize, one homolog from linseed, two sequences from rice and one sequence from sunflower were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 41-48 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).
  • the full-length DNA sequence of the ZM59202533 (SEQ ID NO: 91) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e ⁇ 10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Two homologs from canola and one homolog from maize were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 49-51 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).
  • the polynucleotides of Table 1 are ligated into a binary vector containing a selectable marker.
  • the resulting recombinant vector contains the corresponding gene in the sense orientation under a constitutive promoter.
  • the recombinant vectors are transformed into an Agrobacterium tumefaciens strain according to standard conditions.
  • A. thaliana ecotype Col-0 or C24 are grown and transformed according to standard conditions.
  • T1 and T2 plants are screened for resistance to the selection agent conferred by the selectable marker gene.
  • T3 seeds are used in greenhouse or growth chamber experiments.
  • seeds are refrigerated for stratification. Seeds are then planted, fertilizer is applied and humidity is maintained using transparent domes. Plants are grown in a greenhouse at 22° C. with photoperiod of 16 hours light/8 hours dark. Plants are watered twice a week.
  • plant area, leaf area, biomass, color distribution, color intensity, and growth rate for each plant are measured using a commercially available imaging system.
  • Biomass is calculated as the total plant leaf area at the last measuring time point.
  • Growth rate is calculated as the plant leaf area at the last measuring time point minus the plant leaf area at the first measuring time point divided by the plant leaf area at the first measuring time point.
  • Health index is calculated as the dark green leaf area divided by the total plant leaf area.
  • the polynucleotides of Table 1 are ligated into a binary vector containing a selectable marker.
  • the resulting recombinant vector contains the corresponding gene in the sense orientation under a constitutive promoter.
  • the recombinant vectors are transformed into an A. tumefaciens strain according to standard conditions.
  • A. thaliana ecotype Col-0 or C24 are grown and transformed according to standard conditions.
  • T1 and T2 plants are screened for resistance to the selection agent conferred by the selectable marker gene.
  • Plants are grown in flats using a substrate that contains no organic components. Each flat is wet with water before seedlings resistant to the selection agent are transplanted onto substrate. Plants are grown in a growth chamber set to 22° C. with a 55% relative humidity with photoperiod set at 16 h light/8 h dark. A controlled low or high nitrogen nutrient solution is added to waterings on Days 12, 15, 22 and 29. Watering without nutrient solution occurs on Days 18, 25, and 32. Images of all plants in a tray are taken on days 26, 30, and 33 using a commercially available imaging system. At each imaging time point, biomass and plant phenotypes for each plant are measured including plant area, leaf area, biomass, color distribution, color intensity, and growth rate.
  • Canola cotyledonary petioles of 4 day-old young seedlings are used as explants for tissue culture and transformed according to EP1566443.
  • the commercial cultivar Westar (Agriculture Canada) is the standard variety used for transformation, but other varieties can be used.
  • A. tumefaciens GV3101:pMP90RK containing a binary vector is used for canola transformation.
  • the standard binary vector used for transformation is pSUN (WO02/00900), but many different binary vector systems have been described for plant transformation (e.g. An, G. in Agrobacterium Protocols, Methods in Molecular Biology vol 44, pp 47-62, Gartland K M A and M R Davey eds.
  • a plant gene expression cassette comprising a selection marker gene, a plant promoter, and a polynucleotide of Table 1 is employed.
  • selection marker genes can be used including the mutated acetohydroxy acid synthase (AHAS) gene disclosed in U.S. Pat. Nos. 5,767,366 and 6,225,105.
  • a suitable promoter is used to regulate the trait gene to provide constitutive, developmental, tissue or environmental regulation of gene transcription.
  • Canola seeds are surface-sterilized in 70% ethanol for 2 min, incubated for 15 min in 55° C. warm tap water and then in 1.5% sodium hypochlorite for 10 minutes, followed by three rinses with sterilized distilled water. Seeds are then placed on MS medium without hormones, containing Gamborg B5 vitamins, 3% sucrose, and 0.8% Oxoidagar. Seeds are germinated at 24° C. for 4 days in low light ( ⁇ 50 ⁇ Mol/m 2 s, 16 hours light).
  • the cotyledon petiole explants with the cotyledon attached are excised from the in vitro seedlings, and inoculated with Agrobacterium by dipping the cut end of the petiole explant into the bacterial suspension.
  • the explants are then cultured for 3 days on MS medium including vitamins containing 3.75 mg/l BAP, 3% sucrose, 0.5 g/l MES, pH 5.2, 0.5 mg/l GA3, 0.8% Oxoidagar at 24° C., 16 hours of light.
  • the petiole explants are transferred to regeneration medium containing 3.75 mg/l BAP, 0.5 mg/l GA3, 0.5 g/l MES, pH 5.2, 300 mg/l timentin and selection agent until shoot regeneration.
  • the petiole explants are transferred to shoot elongation medium (A6, containing full strength MS medium including vitamins, 2% sucrose, 0.5% Oxoidagar, 100 mg/l myo-inositol, 40 mg/l adenine sulfate, 0.5 WI MES, pH 5.8, 0.0025 mg/l BAP, 0.1 mg/l IBA, 300 mg/l timentin and selection agent).
  • Samples from both in vitro and greenhouse material of the primary transgenic plants are analyzed by qPCR using TaqMan probes to confirm the presence of T-DNA and to determine the number of T-DNA integrations.
  • Seed is produced from the primary transgenic plants by self-pollination.
  • the second-generation plants are grown in greenhouse conditions and self-pollinated.
  • the plants are analyzed by qPCR using TaqMan probes to confirm the presence of T-DNA and to determine the number of T-DNA integrations.
  • Homozygous transgenic, heterozygous transgenic and azygous (null transgenic) plants are compared for their stress tolerance, for example, in the assays described in Examples 2 and 3, and for yield, both in the greenhouse and in field studies.
  • Transgenic rice plants comprising a polynucleotide of Table 1 are generated using known methods. Approximately 15 to 20 independent transformants (T0) are generated. The primary transformants are transferred from tissue culture chambers to a greenhouse for growing and harvest of T1 seeds. Five events of the T1 progeny segregated 3:1 for presence/absence of the transgene are retained. For each of these events, 10 T1 seedlings containing the transgene (hetero- and homozygotes), and 10 T1 seedlings lacking the transgene (nullizygotes) are selected by visual marker screening. The selected T1 plants are transferred to a greenhouse. Each plant receives a unique barcode label to link unambiguously the phenotyping data to the corresponding plant.
  • Transgenic plants and the corresponding nullizygotes are grown side-by-side at random positions. From the stage of sowing until the stage of maturity, the plants are passed several times through a digital imaging cabinet. At each time point digital, images (2048 ⁇ 1536 pixels, 16 million colours) of each plant are taken from at least 6 different angles.
  • T1 plants The data obtained in the first experiment with T1 plants are confirmed in a second experiment with T2 plants. Lines that have the correct expression pattern are selected for further analysis. Seed batches from the positive plants (both hetero- and homozygotes) in T1 are screened by monitoring marker expression. For each chosen event, the heterozygote seed batches are then retained for T2 evaluation. Within each seed batch, an equal number of positive and negative plants are grown in the greenhouse for evaluation.
  • Transgenic plants are screened for their improved growth and/or yield and/or stress tolerance, for example, using the assays described in Examples 2 and 3, and for yield, both in the greenhouse and in field studies.
  • transgenic plants generated are then screened for their improved growth under water-limited conditions and/or drought, salt, and/or cold tolerance, for example, using the assays described in Examples 2 and 3, and for yield, both in the greenhouse and in field studies.
  • the polynucleotides of Table 1 are transformed into wheat using the method described by Ishida et al., 1996, Nature Biotech. 14745-50. Immature embryos are co-cultivated with Agrobacterium tumefaciens that carry “super binary” vectors, and transgenic plants are recovered through organogenesis. This procedure provides a transformation efficiency between 2.5% and 20%. The transgenic plants are then screened for their improved growth and/or yield under water-limited conditions and/or stress tolerance, for example, is the assays described in Examples 2 and 3, and for yield, both in the greenhouse and in field studies.
  • the polynucleotides of Table 1 are transformed into immature embryos of corn using Agrobacterium . After imbibition, embryos are transferred to medium without selection agent. Seven to ten days later, embryos are transferred to medium containing selection agent and grown for 4 weeks (two 2-week transfers) to obtain transformed callus cells. Plant regeneration is initiated by transferring resistant calli to medium supplemented with selection agent and grown under light at 25-27° C. for two to three weeks. Regenerated shoots are then transferred to rooting box with medium containing selection agent. Plantlets with roots are transferred to potting mixture in small pots in the greenhouse and after acclimatization are then transplanted to larger pots and maintained in greenhouse till maturity.
  • each of these plants is uniquely labeled, sampled and analyzed for transgene copy number.
  • Transgene positive and negative plants are marked and paired with similar sizes for transplanting together to large pots. This provides a uniform and competitive environment for the transgene positive and negative plants.
  • the large pots are watered to a certain percentage of the field water capacity of the soil depending the severity of water-stress desired.
  • the soil water level is maintained by watering every other day.
  • Plant growth and physiology traits such as height, stem diameter, leaf rolling, plant wilting, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured during the growth period. After a period of growth, the above ground portion of the plants is harvested, and the fresh weight and dry weight of each plant are taken. A comparison of the drought tolerance phenotype between the transgene positive and negative plants is then made.
  • the pots are covered with caps that permit the seedlings to grow through but minimize water loss.
  • Each pot is weighed periodically and water added to maintain the initial water content.
  • the fresh and dry weight of each plant is measured, the water consumed by each plant is calculated and WUE of each plant is computed.
  • Plant growth and physiology traits such as WUE, height, stem diameter, leaf rolling, plant wilting, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured during the experiment. A comparison of WUE phenotype between the transgene positive and negative plants is then made.
  • these pots are kept in an area in the greenhouse that has uniform environmental conditions, and cultivated optimally. Each of these plants is uniquely labeled, sampled and analyzed for transgene copy number. The plants are allowed to grow under theses conditions until they reach a predefined growth stage. Water is then withheld. Plant growth and physiology traits such as height, stem diameter, leaf rolling, plant wilting, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured as stress intensity increases. A comparison of the dessication tolerance phenotype between transgene positive and negative plants is then made.
  • Segregating transgenic corn seeds for a transformation event are planted in small pots for testing in a cycling drought assay. These pots are kept in an area in the greenhouse that has uniform environmental conditions, and cultivated optimally. Each of these plants is uniquely labeled, sampled and analyzed for transgene copy number. The plants are allowed to grow under theses conditions until they reach a predefined growth stage. Plants are then repeatedly watered to saturation at a fixed interval of time. This water/drought cycle is repeated for the duration of the experiment. Plant growth and physiology traits such as height, stem diameter, leaf rolling, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured during the growth period. At the end of the experiment, the plants are harvested for above-ground fresh and dry weight. A comparison of the cycling drought tolerance phenotype between transgene positive and negative plants is then made.
  • Plants that have been genotyped in this manner are also scored for a range of phenotypes related to drought-tolerance, growth and yield. These phenotypes include plant height, grain weight per plant, grain number per plant, ear number per plant, above ground dry-weight, leaf conductance to water vapor, leaf CO 2 uptake, leaf chlorophyll content, photosynthesis-related chlorophyll fluorescence parameters, water use efficiency, leaf water potential, leaf relative water content, stem sap flow rate, stem hydraulic conductivity, leaf temperature, leaf reflectance, leaf light absorptance, leaf area, days to flowering, anthesis-silking interval, duration of grain fill, osmotic potential, osmotic adjustment, root size, leaf extension rate, leaf angle, leaf rolling and survival. All measurements are made with commercially available instrumentation for field physiology, using the standard protocols provided by the manufacturers. Individual plants are used as the replicate unit per event.
  • a null segregant is progeny (or lines derived from the progeny) of a transgenic plant that does not contain the transgene due to Mendelian segregation. Additional replicated paired plots for a particular event are distributed around the trial. A range of phenotypes related to drought-tolerance, growth and yield are scored in the paired plots and estimated at the plot level. When the measurement technique could only be applied to individual plants, these are selected at random each time from within the plot.
  • phenotypes include plant height, grain weight per plant, grain number per plant, ear number per plant, above ground dry-weight, leaf conductance to water vapor, leaf CO 2 uptake, leaf chlorophyll content, photosynthesis-related chlorophyll fluorescence parameters, water use efficiency, leaf water potential, leaf relative water content, stem sap flow rate, stem hydraulic conductivity, leaf temperature, leaf reflectance, leaf light absorptance, leaf area, days to flowering, anthesis-silking interval, duration of grain fill, osmotic potential, osmotic adjustment, root size, leaf extension rate, leaf angle, leaf rolling and survival. All measurements are made with commercially available instrumentation for field physiology, using the standard protocols provided by the manufacturers. Individual plots are used as the replicate unit per event.
  • phenotypes included plant height, grain weight per plant, grain number per plant, ear number per plant, above ground dry-weight, leaf conductance to water vapor, leaf CO 2 uptake, leaf chlorophyll content, photosynthesis-related chlorophyll fluorescence parameters, water use efficiency, leaf water potential, leaf relative water content, stem sap flow rate, stem hydraulic conductivity, leaf temperature, leaf reflectance, leaf light absorptance, leaf area, days to flowering, anthesis-silking interval, duration of grain fill, osmotic potential, osmotic adjustment, root size, leaf extension rate, leaf angle, leaf rolling and survival. All measurements are made with commercially available instrumentation for field physiology, using the standard protocols provided by the manufacturers. Individual plots are used as the replicate unit per event.

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Abstract

Polynucleotides are disclosed which are capable of enhancing a growth, yield under water-limited conditions, and/or increased tolerance to an environmental stress of a plant transformed to contain such polynucleotides. Also provided are methods of using such polynucleotides and transgenic plants and agricultural products, including seeds, containing such polynucleotides as transgenes.

Description

  • This application claims priority benefit of U.S. provisional patent application Ser. No. 60/959,346, filed Jul. 13, 2007, the contents of which are hereby incorporated by reference.
    • FIELD OF THE INVENTION
  • This invention relates generally to transgenic plants which overexpress nucleic acid sequences encoding polypeptides capable of conferring increased stress tolerance and consequently, increased plant growth and crop yield, under normal or abiotic stress conditions. Additionally, the invention relates to novel isolated nucleic acid sequences encoding polypeptides that confer upon a plant increased tolerance under abiotic stress conditions, and/or increased plant growth and/or increased yield under normal or abiotic stress conditions.
    • BACKGROUND OF THE INVENTION
  • Abiotic environmental stresses, such as drought, salinity, heat, and cold, are major limiting factors of plant growth and crop yield. Crop yield is defined herein as the number of bushels of relevant agricultural product (such as grain, forage, or seed) harvested per acre. Crop losses and crop yield losses of major crops such as soybean, rice, maize (corn), cotton, and wheat caused by these stresses represent a significant economic and political factor and contribute to food shortages in many underdeveloped countries.
  • Water availability is an important aspect of the abiotic stresses and their effects on plant growth. Continuous exposure to drought conditions causes major alterations in the plant metabolism which ultimately lead to cell death and consequently to yield losses. Because high salt content in some soils results in less water being available for cell intake, high salt concentration has an effect on plants similar to the effect of drought on plants. Additionally, under freezing temperatures, plant cells lose water as a result of ice formation within the plant. Accordingly, crop damage from drought, heat, salinity, and cold stress, is predominantly due to dehydration.
  • Because plants are typically exposed to conditions of reduced water availability during their life cycle, most plants have evolved protective mechanisms against desiccation caused by abiotic stresses. However, if the severity and duration of desiccation conditions are too great, the effects on development, growth, plant size, and yield of most crop plants are profound. Developing plants efficient in water use is therefore a strategy that has the potential to significantly improve human life on a worldwide scale.
  • Traditional plant breeding strategies are relatively slow and require abiotic stress-tolerant founder lines for crossing with other germplasm to develop new abiotic stress-resistant lines. Limited germplasm resources for such founder lines and incompatibility in crosses between distantly related plant species represent significant problems encountered in conventional breeding. Breeding for tolerance has been largely unsuccessful.
  • Many agricultural biotechnology companies have attempted to identify genes that could confer tolerance to abiotic stress responses, in an effort to develop transgenic abiotic stress-tolerant crop plants. Although some genes that are involved in stress responses or water use efficiency in plants have been characterized, the characterization and cloning of plant genes that confer stress tolerance and/or water use efficiency remains largely incomplete and fragmented. To date, success at developing transgenic abiotic stress-tolerant crop plants has been limited, and no such plants have been commercialized.
  • In order to develop transgenic abiotic stress-tolerant crop plants, it is necessary to assay a number of parameters in model plant systems, greenhouse studies of crop plants, and in field trials. For example, water use efficiency (WUE), is a parameter often correlated with drought tolerance. Studies of a plant's response to desiccation, osmotic shock, and temperature extremes are also employed to determine the plant's tolerance or resistance to abiotic stresses. When testing for the impact of the presence of a transgene on a plant's stress tolerance, the ability to standardize soil properties, temperature, water and nutrient availability and light intensity is an intrinsic advantage of greenhouse or plant growth chamber environments compared to the field.
  • WUE has been defined and measured in multiple ways. One approach is to calculate the ratio of whole plant dry weight, to the weight of water consumed by the plant throughout its life. Another variation is to use a shorter time interval when biomass accumulation and water use are measured. Yet another approach is to use measurements from restricted parts of the plant, for example, measuring only aerial growth and water use. WUE also has been defined as the ratio of CO2 uptake to water vapor loss from a leaf or portion of a leaf, often measured over a very short time period (e.g. seconds/minutes). The ratio of 13C/12C fixed in plant tissue, and measured with an isotope ratio mass-spectrometer, also has been used to estimate WUE in plants using C3 photosynthesis.
  • An increase in WUE is informative about the relatively improved efficiency of growth and water consumption, but this information taken alone does not indicate whether one of these two processes has changed or both have changed. In selecting traits for improving crops, an increase in WUE due to a decrease in water use, without a change in growth would have particular merit in an irrigated agricultural system where the water input costs were high. An increase in WUE driven mainly by an increase in growth without a corresponding jump in water use would have applicability to all agricultural systems. In many agricultural systems where water supply is not limiting, an increase in growth, even if it came at the expense of an increase in water use (i.e. no change in WUE), could also increase yield. Therefore, new methods to increase both WUE and biomass accumulation are required to improve agricultural productivity.
  • Concomitant with measurements of parameters that correlate with abiotic stress tolerance are measurements of parameters that indicate the potential impact of a transgene on crop yield. For forage crops like alfalfa, silage corn, and hay, the plant biomass correlates with the total yield. For grain crops, however, other parameters have been used to estimate yield, such as plant size, as measured by total plant dry weight, above-ground dry weight, above-ground fresh weight, leaf area, stem volume, plant height, rosette diameter, leaf length, root length, root mass, tiller number, and leaf number. Plant size at an early developmental stage will typically correlate with plant size later in development. A larger plant with a greater leaf area can typically absorb more light and carbon dioxide than a smaller plant and therefore will likely gain a greater weight during the same period. This is in addition to the potential continuation of the micro-environmental or genetic advantage that the plant had to achieve the larger size initially. There is a strong genetic component to plant size and growth rate, and so for a range of diverse genotypes plant size under one environmental condition is likely to correlate with size under another. In this way a standard environment is used to approximate the diverse and dynamic environments encountered at different locations and times by crops in the field.
  • Harvest index, the ratio of seed yield to above-ground dry weight, is relatively stable under many environmental conditions and so a robust correlation between plant size and grain yield is possible. Plant size and grain yield are intrinsically linked, because the majority of grain biomass is dependent on current or stored photosynthetic productivity by the leaves and stem of the plant. Therefore, selecting for plant size, even at early stages of development, has been used as to screen for plants that may demonstrate increased yield when exposed to field testing. As with abiotic stress tolerance, measurements of plant size in early development, under standardized conditions in a growth chamber or greenhouse, are standard practices to measure potential yield advantages conferred by the presence of a transgene.
  • There is a need, therefore, to identify additional genes expressed in stress tolerant plants and/or plants that are efficient in water use that have the capacity to confer stress tolerance and/or increased water use efficiency to the host plant and to other plant species. Newly generated stress tolerant plants and/or plants with increased water use efficiency will have many advantages, such as an increased range in which the crop plants can be cultivated, by for example, decreasing the water requirements of a plant species. Other desirable advantages include increased resistance to lodging, the bending of shoots or stems in response to wind, rain, pests, or disease.
    • SUMMARY OF THE INVENTION
  • The present inventors have discovered that transforming a plant with certain polynucleotides results in enhancement of the plant's growth and response to environmental stress, and accordingly the yield of the agricultural products of the plant is increased, when the polynucleotides are present in the plant as transgenes. The polynucleotides capable of mediating such enhancements have been isolated from Arabidopsis thaliana, Capsicum annuum, Escherichia coil, Physcomitrella patens, Saccharomyces cerevisiae, Triticum aestivum, Zea mays, Glycine max, Linum usitatissimum, Triticum aestivum, Oryza sativa, Helianthus annuus, and Brassica napus and the sequences thereof are set forth in the Sequence Listing as indicated in Table 1.
  • TABLE 1
    Polynucleotide Amino acid
    Gene Name Organism SEQ ID NO SEQ ID NO
    At2g20725 A. thaliana 1 2
    At3g26085 A. thaliana 3 4
    AtFACE-2 A. thaliana 5 6
    ZM57353913 Z. mays 7 8
    ZM59252659 Z. mays 9 10
    CASAR82A C. annuum 11 12
    b3358 E. coli 13 14
    EST564 P. patens 15 16
    BN49502266 B. napus 17 18
    GM49788080 G. max 19 20
    GM53049821 G. max 21 22
    ZM58462719 Z. mays 23 24
    ZM61092633 Z. mays 25 26
    ZM62016485 Z. mays 27 28
    ZM62051019 Z. mays 29 30
    ZM65086957 Z. mays 31 32
    ZM68587657 Z. mays 33 34
    EST390 P. patens 35 36
    BN51363030 B. napus 37 38
    BN42986056 B. napus 39 40
    BN49389066 B. napus 41 42
    BN51339479 B. napus 43 44
    ZM57651070 Z. mays 45 46
    ZM62073276 Z. mays 47 48
    EST257 P. patens 49 50
    LU61665952 L. usitatissimum 51 52
    TA56863186 T. aestivum 53 54
    ZM62026837 Z. mays 55 56
    ZM65457595 Z. mays 57 58
    ZM67230154 Z. mays 59 60
    EST465 P. patens 61 62
    YBL109w S. cerevisiae 63 64
    YBL100c S. cerevisiae 65 66
    YKL184w S. cerevisiae 67 68
    YPL091w S. cerevisiae 69 70
    TA54587433 T. aestivum 71 72
    ZM68532504 Z. mays 73 74
    BN42856089 B. napus 75 76
    BN43206527 B. napus 77 78
    HA66872964 H. annuus 79 80
    LU61662612 L. usitatissimum 81 82
    OS32806943 O. sativa 83 84
    OS34738749 O. sativa 85 86
    ZM59400933 Z. mays 87 88
    ZM62132060 Z. mays 89 90
    ZM59202533 Z. mays 91 92
    BN41901422 B. napus 93 94
    BN47868329 B. napus 95 96
    BN42671700 B. napus 97 98
    ZM68416988 Z. mays 99 100
  • In one embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a CAAX amino terminal protease family protein.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a prenyl-dependent CAAX protease.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a SAR8.2 protein precursor.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a putative membrane protein.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein phosphatase 2C protein.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a mitochondrial carrier protein.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein kinase.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a peptidyl prolyl isomerase.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a unknown protein 1.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an Isolated polynucleotide encoding a unknown protein 2.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a ornithine decarboxylase.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a glutathione reductase.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a unknown protein 3.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein phosphatase 2A protein.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a MEK1 protein kinase.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a AP2 domain containing transcription factor.
  • In a further embodiment, the invention concerns a seed produced by the transgenic plant of the invention, wherein the seed is true breeding for a transgene comprising the polynucleotide described above. Plants derived from the seed of the invention demonstrate increased tolerance to an environmental stress, and/or increased plant growth, and/or increased yield, under normal or stress conditions as compared to a wild type variety of the plant.
  • In a still another aspect, the invention concerns products produced by or from the transgenic plants of the invention, their plant parts, or their seeds, such as a foodstuff, feedstuff, food supplement, feed supplement, cosmetic or pharmaceutical.
  • The invention further provides certain isolated polynucleotides identified in Table 1, and certain isolated polypeptides identified in Table 1. The invention is also embodied in recombinant vector comprising an isolated polynucleotide of the invention.
  • In yet another embodiment, the invention concerns a method of producing the aforesaid transgenic plant, wherein the method comprises transforming a plant cell with an expression vector comprising an isolated polynucleotide of the invention, and generating from the plant cell a transgenic plant that expresses the polypeptide encoded by the polynucleotide. Expression of the polypeptide in the plant results in increased tolerance to an environmental stress, and/or growth, and/or yield under normal and/or stress conditions as compared to a wild type variety of the plant.
  • In still another embodiment, the invention provides a method of increasing a plant's tolerance to an environmental stress, and/or growth, and/or yield. The method comprises the steps of transforming a plant cell with an expression cassette comprising an isolated polynucleotide of the invention, and generating a transgenic plant from the plant cell, wherein the transgenic plant comprises the polynucleotide.
    • BRIEF DESCRIPTION OF THE DRAWINGS
  • FIG. 1 shows an alignment of the disclosed amino acid sequences AtFACE-2 (SEQ ID NO:6), ZM57353913 (SEQ ID NO:8), and ZM59252659 (SEQ ID NO:10). The alignment was generated using Align X of Vector NTI.
  • FIG. 2 shows an alignment of the disclosed amino acid sequences EST564 (SEQ ID NO:16), BN49502266 (SEQ ID NO:18), GM49788080 (SEQ ID NO:20), GM53049821 (SEQ ID NO:22), ZM58462719 (SEQ ID NO:24), ZM61092633 (SEQ ID NO:26), ZM62016485 (SEQ ID NO:28), ZM62051019 (SEQ ID NO:30), ZM65086957 (SEQ ID NO:32), and ZM68587657 (SEQ ID NO:34). The alignment was generated using Align X of Vector NTI.
  • FIG. 3 shows an alignment of the disclosed amino acid sequences EST390 (SEQ ID NO:36), BN51363030 (SEQ ID NO:38), BN42986056 (SEQ ID NO:40), BN49389066 (SEQ ID NO:42), BN51339479 (SEQ ID NO:44), ZM57651070 (SEQ ID NO:46), and ZM62073276 (SEQ ID NO:48). The alignment was generated using Align X of Vector NTI.
  • FIG. 4 shows an alignment of the disclosed amino acid sequences EST257 (SEQ ID NO:50), LU61665952 (SEQ ID NO:52), TA56863186 (SEQ ID NO:54), ZM62026837 (SEQ ID NO:56), ZM65457595 (SEQ ID NO:58), ZM67230154 (SEQ ID NO:60). The alignment was generated using Align X of Vector NTI.
  • FIG. 5 shows an alignment of the disclosed amino acid sequences ZM68532504 (SEQ ID NO:74), BN42856089 (SEQ ID NO:76), BN43206527 (SEQ ID NO:78), HA66872964 (SEQ ID NO:80), LU61662612 (SEQ ID NO:82), 0832806943 (SEQ ID NO:84), OS34738749 (SEQ ID NO:86), ZM59400933 (SEQ ID NO:88), and ZM62132060 (SEQ ID NO:90). The alignment was generated using Align X of Vector NTI.
  • FIG. 6 shows an alignment of the disclosed amino acid sequences ZM59202533 (SEQ ID NO:92), 6N41901422 (SEQ ID NO:94), BN47868329 (SEQ ID NO:96), and ZM68416988 (SEQ ID NO:100). The alignment was generated using Align X of Vector NTI.
    •  DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
  • Throughout this application, various publications are referenced. The disclosures of all of these publications and those references cited within those publications in their entireties are hereby incorporated by reference into this application in order to more fully describe the state of the art to which this invention pertains. The terminology used herein is for the purpose of describing specific embodiments only and is not intended to be limiting. As used herein, “a” or “an” can mean one or more, depending upon the context in which it is used. Thus, for example, reference to “a cell” can mean that at least one cell can be used.
  • In one embodiment, the invention provides a transgenic plant that overexpresses an isolated polynucleotide identified in Table 1, or a homolog thereof. The transgenic plant of the invention demonstrates an increased tolerance to an environmental stress as compared to a wild type variety of the plant. The overexpression of such isolated nucleic acids in the plant may optionally result in an increase in plant growth or in yield of associated agricultural products, under normal or stress conditions, as compared to a wild type variety of the plant.
  • As defined herein, a “transgenic plant” is a plant that has been altered using recombinant DNA technology to contain an isolated nucleic acid which would otherwise not be present in the plant. As used herein, the term “plant” includes a whole plant, plant cells, and plant parts. Plant parts include, but are not limited to, stems, roots, ovules, stamens, leaves, embryos, meristematic regions, callus tissue, gametophytes, sporophytes, pollen, microspores, and the like. The transgenic plant of the invention may be male sterile or male fertile, and may further include transgenes other than those that comprise the isolated polynucleotides described herein.
  • As used herein, the term “variety” refers to a group of plants within a species that share constant characteristics that separate them from the typical form and from other possible varieties within that species. While possessing at least one distinctive trait, a variety is also characterized by some variation between individuals within the variety, based primarily on the Mendelian segregation of traits among the progeny of succeeding generations. A variety is considered “true breeding” for a particular trait if it is genetically homozygous for that trait to the extent that, when the true-breeding variety is self-pollinated, a significant amount of independent segregation of the trait among the progeny is not observed. In the present invention, the trait arises from the transgenic expression of one or more isolated polynucleotides introduced into a plant variety. As also used herein, the term “wild type variety” refers to a group of plants that are analyzed for comparative purposes as a control plant, wherein the wild type variety plant is identical to the transgenic plant (plant transformed with an isolated polynucleotide in accordance with the invention) with the exception that the wild type variety plant has not been transformed with an isolated polynucleotide of the invention.
  • As defined herein, the term “nucleic acid” and “polynucleotide” are interchangeable and refer to RNA or DNA that is linear or branched, single or double stranded, or a hybrid thereof. The term also encompasses RNA/DNA hybrids. An “isolated” nucleic acid molecule is one that is substantially separated from other nucleic acid molecules which are present in the natural source of the nucleic acid (i.e., sequences encoding other polypeptides). For example, a cloned nucleic acid is considered isolated. A nucleic acid is also considered isolated if it has been altered by human intervention, or placed in a locus or location that is not its natural site, or if it is introduced into a cell by transformation. Moreover, an isolated nucleic acid molecule, such as a cDNA molecule, can be free from some of the other cellular material with which it is naturally associated, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized. While it may optionally encompass untranslated sequence located at both the 3′ and 5′ ends of the coding region of a gene, it may be preferable to remove the sequences which naturally flank the coding region in its naturally occurring replicon.
  • As used herein, the term “environmental stress” refers to a sub-optimal condition associated with salinity, drought, nitrogen, temperature, metal, chemical, pathogenic, or oxidative stresses, or any combination thereof. The terms “water use efficiency” and “WUE” refer to the amount of organic matter produced by a plant divided by the amount of water used by the plant in producing it, i.e., the dry weight of a plant in relation to the plant's water use. As used herein, the term “dry weight” refers to everything in the plant other than water, and includes, for example, carbohydrates, proteins, oils, and mineral nutrients.
  • Any plant species may be transformed to create a transgenic plant in accordance with the invention. The transgenic plant of the invention may be a dicotyledonous plant or a monocotyledonous plant. For example and without limitation, transgenic plants of the invention may be derived from any of the following dicotyledonous plant families: Leguminosae, including plants such as pea, alfalfa and soybean; Umbelliferae, including plants such as carrot and celery; Solanaceae, including the plants such as tomato, potato, aubergine, tobacco, and pepper; Cruciferae, particularly the genus Brassica, which includes plant such as oilseed rape, beet, cabbage, cauliflower and broccoli); and A. thaliana; Compositae, which includes plants such as lettuce; Malvaceae, which includes cotton; Fabaceae, which includes plants such as peanut, and the like. Transgenic plants of the invention may be derived from monocotyledonous plants, such as, for example, wheat, barley, sorghum, millet, rye, triticale, maize, rice, oats and sugarcane. Transgenic plants of the invention are also embodied as trees such as apple, pear, quince, plum, cherry, peach, nectarine, apricot, papaya, mango, and other woody species including coniferous and deciduous trees such as poplar, pine, sequoia, cedar, oak, and the like. Especially preferred are Arabidopsis thaliana, Nicotiana tabacum, oilseed rape, soybean, corn (maize), wheat, linseed, potato and tagetes.
  • As shown in Table 1, one embodiment of the invention is a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a CAAX amino terminal protease family protein. The transgenic plant of this embodiment may comprise any polynucleotide encoding a CAAX amino terminal protease family protein. The transgenic plant of this embodiment comprises a polynucleotide encoding a CAAX amino terminal protease family protein having a sequence comprising amino acids 1 to 301 of SEQ ID NO:2; and a protein having a sequence comprising amino acids 1 to 293 of SEQ ID NO:4.
  • In another embodiment, the Invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a prenyl-dependent CAAX protease. The transgenic'plant of this embodiment may comprise any polynucleotide encoding a prenyl-dependent CAAX protease. The transgenic plant of this embodiment comprises a polynucleotide encoding a prenyl-dependent CAAX protease having a sequence comprising amino acids 1 to 311 of SEQ ID NO:6; a protein having a sequence comprising amino acids 1 to 313 of SEQ ID NO:8; a protein having a sequence comprising amino acids 1 to 269 of SEQ ID NO:10.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a SAR8.2 protein precursor. The transgenic plant of this embodiment may comprise any polynucleotide encoding a SAR8.2 protein precursor. The transgenic plant of this embodiment comprises a polynucleotide encoding a SAR8.2 protein precursor having a sequence comprising amino acids 1 to 86 of SEQ ID NO:12.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a putative membrane protein. The transgenic plant of this embodiment may comprise any polynucleotide encoding a putative membrane protein. The transgenic plant of this embodiment comprises a polynucleotide encoding a putative membrane protein having a sequence comprising amino acids 1 to 696 of SEQ ID NO:14.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein phosphatase 2C protein. The transgenic plant of this embodiment may comprise any polynucleotide encoding a protein phosphatase 2C protein. The transgenic plant of this embodiment comprises a polynucleotide encoding a protein phosphatase 2C protein having a sequence comprising amino acids 1 to 284 of SEQ ID NO:16; a protein having a sequence comprising amino acids 1 to 384 of SEQ ID NO:18; a protein having a sequence comprising amino acids 1 to 346 of SEQ ID NO:20; a protein having a sequence comprising amino acids 1 to 375 of SEQ ID NO:22; a protein having a sequence comprising amino acids 1 to 390 of SEQ ID NO:24; a protein having a sequence comprising amino acids 1 to 398 of SEQ ID NO:26; a protein having a sequence comprising amino acids 1 to 399 of SEQ ID NO:28; a protein having a sequence comprising amino acids 1 to 399 of SEQ ID NO:30; a protein having a sequence comprising amino acids 1 to 399 of SEQ ID NO:32; a protein having a sequence comprising amino acids 1 to 276 of SEQ ID NO:34.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a mitochondrial carrier protein. The transgenic plant of this embodiment may comprise any polynucleotide encoding a mitochondrial carrier protein. The transgenic plant of this embodiment comprises a polynucleotide encoding a mitochondrial carrier protein having a sequence comprising amino acids 1 to 303 of SEQ ID NO:36; a protein having a sequence comprising amino acids 1 to 315 of SEQ ID NO:38; a protein having a sequence comprising amino acids 1 to 289 of SEQ ID NO:40; a protein having a sequence comprising amino acids 1 to 303 of SEQ ID NO:42; a protein having a sequence comprising amino acids 1 to 299 of SEQ ID NO:44; a protein having a sequence comprising amino acids 1 to 299 of SEQ ID NO:46; a protein having a sequence comprising amino acids 1 to 311 of SEQ ID NO:48.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein kinase. The transgenic plant of this embodiment may comprise any polynucleotide encoding a protein kinase. The transgenic plant of this embodiment comprises a polynucleotide encoding a protein kinase having a sequence comprising amino acids 1 to 356 of SEQ ID NO:50; a protein having a sequence comprising amino acids 1 to 364 of SEQ ID NO:52; a protein having a sequence comprising amino acids 1 to 361 of SEQ ID NO:54; a protein having a sequence comprising amino acids 1 to 370 of SEQ ID NO:56; a protein having a sequence comprising amino acids 1 to 377 of SEQ ID NO:58; a protein having a sequence comprising amino acids 1 to 382 of SEQ ID NO:60.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a peptidyl prolyl isomerase. The transgenic plant of this embodiment may comprise any polynucleotide encoding a peptidyl prolyl isomerase. The transgenic plant of this embodiment comprises a polynucleotide encoding a peptidyl prolyl isomerase having a sequence comprising amino acids 1 to 523 of SEQ ID NO:62.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding an unknown protein 1. The transgenic plant of this embodiment may comprise any polynucleotide encoding an unknown protein 1. The transgenic plant of this embodiment comprises a polynucleotide encoding a unknown protein 1 having a sequence comprising amino acids 1 to 111 of SEQ ID NO:64.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding an unknown protein 2. The transgenic plant of this embodiment may comprise any polynucleotide encoding an unknown protein 2. The transgenic plant of this embodiment comprises a polynucleotide encoding a unknown protein 2 having a sequence comprising amino acids 1 to 104 of SEQ ID NO:66.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a ornithine decarboxylase. The transgenic plant of this embodiment may comprise any polynucleotide encoding a ornithine decarboxylase. The transgenic plant of this embodiment comprises a polynucleotide encoding a ornithine decarboxylase having a sequence comprising amino acids 1 to 466 of SEQ ID NO:68.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a glutathione reductase. The transgenic plant of this embodiment may comprise any polynucleotide encoding a glutathione reductase. The transgenic plant of this embodiment comprises a polynucleotide encoding a glutathione reductase having a sequence comprising amino acids 1 to 483 of SEQ ID NO:70.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding an unknown protein 3. The transgenic plant of this embodiment may comprise any polynucleotide encoding a unknown protein 3. The transgenic plant of this embodiment comprises a polynucleotide encoding a unknown protein 3 having a sequence comprising amino acids 1 to 129 of SEQ ID NO:72.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein phosphatase 2A protein. The transgenic plant of this embodiment may comprise any polynucleotide encoding a protein phosphatase 2A protein. The transgenic plant of this embodiment comprises a polynucleotide encoding a protein phosphatase 2A protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:74; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:76; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:78; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:80; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:82; a protein having a sequence comprising amino acids 1 to 307 of SEQ ID NO:84; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:86; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:88; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:90.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a MEK1 protein kinase. The transgenic plant of this embodiment may comprise any polynucleotide encoding a MEK1 protein kinase. The transgenic plant of this embodiment comprises a polynucleotide encoding a MEK1 protein kinase having a sequence comprising amino acids 1 to 355 of SEQ ID NO:92; a protein having a sequence comprising amino acids 1 to 355 of SEQ ID NO:94; a protein having a sequence comprising amino acids 1 to 338 of SEQ ID NO:96; a protein having a sequence comprising amino acids 1 to 350 of SEQ ID NO:100.
  • In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding an AP2 domain containing transcription factor. The transgenic plant of this embodiment may comprise any polynucleotide encoding a AP2 domain containing transcription factor. The transgenic plant of this embodiment comprises a polynucleotide encoding a AP2 domain containing transcription factor having a sequence comprising amino acids 1 to 197 of SEQ ID NO:98.
  • The invention further provides a seed produced by a transgenic plant expressing polynucleotide listed in Table 1, wherein the seed contains the polynucleotide, and wherein the plant is true breeding for increased growth and/or yield under normal or stress conditions and/or increased tolerance to an environmental stress as compared to a wild type variety of the plant. The invention also provides a product produced by or from the transgenic plants expressing the polynucleotide, their plant parts, or their seeds. The product can be obtained using various methods well known in the art. As used herein, the word “product” includes, but not limited to, a foodstuff, feedstuff, a food supplement, feed supplement, cosmetic or pharmaceutical. Foodstuffs are regarded as compositions used for nutrition or for supplementing nutrition. Animal feedstuffs and animal feed supplements, in particular, are regarded as foodstuffs. The invention further provides an agricultural product produced by any of the transgenic plants, plant parts, and plant seeds. Agricultural products include, but are not limited to, plant extracts, proteins, amino acids, carbohydrates, fats, oils, polymers, vitamins, and the like.
  • In a preferred embodiment, an isolated polynucleotide of the invention comprises a polynucleotide having a sequence selected from the group consisting of the polynucleotide sequences listed in Table 1. These polynucleotides may comprise sequences of the coding region, as well as 5′ untranslated sequences and 3′ untranslated sequences.
  • A polynucleotide of the invention can be isolated using standard molecular biology techniques and the sequence information provided herein, for example, using an automated DNA synthesizer.
  • “Homologs” are defined herein as two nucleic acids or polypeptides that have similar, or substantially identical, nucleotide or amino acid sequences, respectively. Homologs include allelic variants, analogs, and orthologs, as defined below. As used herein, the term “analogs” refers to two nucleic acids that have the same or similar function, but that have evolved separately in unrelated organisms. As used herein, the term “orthologs” refers to two nucleic acids from different species, but that have evolved from a common ancestral gene by speciation. The term homolog further encompasses nucleic acid molecules that differ from one of the nucleotide sequences shown in Table 1 due to degeneracy of the genetic code and thus encode the same polypeptide. As used herein, a “naturally occurring” nucleic acid molecule refers to an RNA or DNA molecule having a nucleotide sequence that occurs in nature (e.g., encodes a natural polypeptide).
  • To determine the percent sequence identity of two amino acid sequences (e.g., one of the polypeptide sequences of Table 1 and a homolog thereof), the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in the sequence of one polypeptide for optimal alignment with the other polypeptide or nucleic acid). The amino acid residues at corresponding amino acid positions are then compared. When a position in one sequence is occupied by the same amino acid residue as the corresponding position in the other sequence then the molecules are identical at that position. The same type of comparison can be made between two nucleic acid sequences.
  • Preferably, the isolated amino acid homologs, analogs, and orthologs of the polypeptides of the present invention are at least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-75%, 75-80%, 80-85%, 85-90%, or 90-95%, and most preferably at least about 96%, 97%, 98%, 99%, or more identical to an entire amino acid sequence identified in Table 1. In another preferred embodiment, an isolated nucleic acid homolog of the invention comprises a nucleotide sequence which is at least about 40-60%, preferably at least about 60-70%, more preferably at least about 70-75%, 75-80%, 80-85%, 85-90%, or 90-95%, and even more preferably at least about 95%, 96%, 97%, 98%, 99%, or more identical to a nucleotide sequence shown in Table 1.
  • For the purposes of the invention, the percent sequence identity between two nucleic acid or polypeptide sequences is determined using the Vector NTI 9.0 (PC) software package (Invitrogen, 1600 Faraday Ave., Carlsbad, Calif. 92008). A gap opening penalty of 15 and a gap extension penalty of 6.66 are used for determining the percent identity of two nucleic acids. A gap opening penalty of 10 and a gap extension penalty of 0.1 are used for determining the percent identity of two polypeptides. All other parameters are set at the default settings. For purposes of a multiple alignment (Clustal W algorithm), the gap opening penalty is 10, and the gap extension penalty is 0.05 with blosum62 matrix. It is to be understood that for the purposes of determining sequence identity when comparing a DNA sequence to an RNA sequence, a thymidine nucleotide is equivalent to a uracil nucleotide.
  • Nucleic acid molecules corresponding to homologs, analogs, and orthologs of the polypeptides listed in Table 1 can be isolated based on their Identity to said polypeptides, using the polynucleotides encoding the respective polypeptides or primers based thereon, as hybridization probes according to standard hybridization techniques under stringent hybridization conditions. As used herein with regard to hybridization for DNA to a DNA blot, the term “stringent conditions” refers to hybridization overnight at 60° C. in 10× Denhart's solution, 6×SSC, 0.5% SDS, and 100 μg/ml denatured salmon sperm DNA. Blots are washed sequentially at 62° C. for 30 minutes each time in 3×SSC/0.1% SDS, followed by 1×SSC/0.1% SDS, and finally 0.1×SSC/0.1% SDS. As also used herein, in a preferred embodiment, the phrase “stringent conditions” refers to hybridization in a 6×SSC solution at 65° C. In another embodiment, “highly stringent conditions” refers to hybridization overnight at 65° C. in 10× Denhart's solution, 6×SSC, 0.5% SDS and 100 μg/ml denatured salmon sperm DNA. Blots are washed sequentially at 65° C. for 30 minutes each time in 3×SSC/0.1% SDS, followed by 1×SSC/0.1% SDS, and finally 0.1×SSC/0.1% SDS. Methods for performing nucleic acid hybridizations are well known in the art. Preferably, an isolated nucleic acid molecule of the invention that hybridizes under stringent or highly stringent conditions to a nucleotide sequence listed in Table 1 corresponds to a naturally occurring nucleic acid molecule.
  • There are a variety of methods that can be used to produce libraries of potential homologs from a degenerate oligonucleotide sequence. Chemical synthesis of a degenerate gene sequence can be performed in an automatic DNA synthesizer, and the synthetic gene is then ligated into an appropriate expression vector. Use of a degenerate set of genes allows for the provision, in one mixture, of all of the sequences encoding the desired set of potential sequences. Methods for synthesizing degenerate oligonucleotides are known in the art.
  • Additionally, optimized nucleic acids can be created. Preferably, an optimized nucleic acid encodes a polypeptide that has a function similar to those of the polypeptides listed in Table 1 and/or modulates a plant's growth and/or yield under normal and/or water-limited conditions and/or tolerance to an environmental stress, and more preferably increases a plant's growth and/or yield under normal and/or water-limited conditions and/or tolerance to an environmental stress upon its overexpression in the plant. As used herein, “optimized” refers to a nucleic acid that is genetically engineered to increase its expression in a given plant or animal. To provide plant optimized nucleic acids, the DNA sequence of the gene can be modified to: 1) comprise codons preferred by highly expressed plant genes; 2) comprise an A+T content in nucleotide base composition to that substantially found in plants; 3) form a plant initiation sequence; 4) to eliminate sequences that cause destabilization, inappropriate polyadenylation, degradation and termination of RNA, or that form secondary structure hairpins or RNA splice sites; or 5) elimination of antisense open reading frames. Increased expression of nucleic acids in plants can be achieved by utilizing the distribution frequency of codon usage in plants in general or in a particular plant. Methods for optimizing nucleic acid expression in plants can be found in EPA 0359472; EPA 0385962; PCT Application No. WO 91/16432; U.S. Pat. No. 5,380,831; U.S. Pat. No. 5,436,391; Perlack et al., 1991, Proc. Natl. Acad. Sci. USA 88:3324-3328; and Murray at al., 1989, Nucleic Acids Res. 17:477-498.
  • An isolated polynucleotide of the invention can be optimized such that its distribution frequency of codon usage deviates, preferably, no more than 25% from that of highly expressed plant genes and, more preferably, no more than about 10%. In addition, consideration is given to the percentage G+C content of the degenerate third base (monocotyledons appear to favor G+C in this position, whereas dicotyledons do not). It is also recognized that the XCG (where X is A, T, C, or G) nucleotide is the least preferred codon in dicots, whereas the XTA codon is avoided in both monocots and dicots. Optimized nucleic acids of this invention also preferably have CG and TA doublet avoidance indices closely approximating those of the chosen host plant. More preferably, these indices deviate from that of the host by no more than about 10-15%.
  • The invention further provides an isolated recombinant expression vector comprising a polynucleotide as described above, wherein expression of the vector in a host cell results in the plant's increased growth and/or yield under normal or water-limited conditions and/or increased tolerance to environmental stress as compared to a wild type variety of the host cell. The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression, which is operatively linked to the nucleic acid sequence to be expressed. As used herein with respect to a recombinant expression vector, “operatively linked” is intended to mean that the nucleotide sequence of interest is linked to the regulatory sequence(s) in a manner which allows for expression of the nucleotide sequence (e.g., in a bacterial or plant host cell when the vector is introduced into the host cell). The term “regulatory sequence” is intended to include promoters, enhancers, and other expression control elements (e.g., polyadenylation signals). Such regulatory sequences are well known in the art. Regulatory sequences include those that direct constitutive expression of a nucleotide sequence in many types of host cells and those that direct expression of the nucleotide sequence only in certain host cells or under certain conditions. It will be appreciated by those skilled in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of polypeptide desired, etc. The expression vectors of the invention can be introduced into host cells to thereby produce polypeptides encoded by nucleic acids as described herein.
  • Plant gene expression should be operatively linked to an appropriate promoter conferring gene expression in a timely, cell specific, or tissue specific manner. Promoters useful in the expression cassettes of the invention include any promoter that is capable of initiating transcription in a plant cell. Such promoters include, but are not limited to, those that can be obtained from plants, plant viruses, and bacteria that contain genes that are expressed in plants, such as Agrobacterium and Rhizobium.
  • The promoter may be constitutive, inducible, developmental stage-preferred, cell type-preferred, tissue-preferred, or organ-preferred. Constitutive promoters are active under most conditions. Examples of constitutive promoters include the CaMV 19S and 35S promoters, the sX CaMV 355 promoter, the Sep1 promoter, the rice actin promoter, the Arabidopsis actin promoter, the ubiquitan promoter, pEmu, the figwort mosaic virus 35S promoter, the Smas promoter, the super promoter (U.S. Pat. No. 5,955,646), the GRP1-8 promoter, the cinnamyl alcohol dehydrogenase promoter (U.S. Pat. No. 5,683,439), promoters from the T-DNA of Agrobacterium, such as mannopine synthase, nopaline synthase, and octopine synthase, the small subunit of ribulose biphosphate carboxylase (ssu-RUBISCO) promoter, and the like.
  • Inducible promoters are preferentially active under certain environmental conditions, such as the presence or absence of a nutrient or metabolite, heat or cold, light, pathogen attack, anaerobic conditions, and the like. For example, the hsp80 promoter from Brassica is induced by heat shock; the PPDK promoter is induced by light; the PR-1 promoters from tobacco, Arabidopsis, and maize are inducible by infection with a pathogen; and the Adh1 promoter is Induced by hypoxia and cold stress. Plant gene expression can also be facilitated via an inducible promoter (For a review, see Gatz, 1997, Annu. Rev. Plant Physiol. Plant Mol. Biol. 48:89-108). Chemically inducible promoters are especially suitable if gene expression is wanted to occur in a time specific manner. Examples of such promoters are a salicylic acid inducible promoter (PCT Application No. WO 95/19443), a tetracycline inducible promoter (Gatz et al., 1992, Plant J. 2: 397-404), and an ethanol inducible promoter (PCT Application No. WO 93/21334).
  • In one preferred embodiment of the present invention, the inducible promoter is a stress-inducible promoter. For the purposes of the invention, stress-inducible promoters are preferentially active under one or more of the following stresses: sub-optimal conditions associated with salinity, drought, nitrogen, temperature, metal, chemical, pathogenic, and oxidative stresses. Stress inducible promoters include, but are not limited to, Cor78 (Chak et al., 2000, Planta 210:875-883; Hovath et al., 1993, Plant Physiol. 103:1047-1053), Cor15a (Artus et al., 1996, PNAS 93(23):13404-09), Rci2A (Medina et al., 2001, Plant Physiol. 125:1655-66; Nylander at al., 2001, Plant Mol. Biol. 45:341-52; Navarre and Goffeau, 2000, EMBO J. 19:2515-24; Capel et al., 1997, Plant Physiol. 115:569-76), Rd22 (Xiong et al., 2001, Plant Cell 13:2063-83; Abe et al., 1997, Plant Cell 9:1859-68; Iwasaki et al., 1995, Mol. Gen. Genet. 247:391-8), cDet6 (Lang and Palve, 1992, Plant Mol. Biol. 20:951-62), ADH1 (Hoeren et al., 1998, Genetics 149:479-90), KAT1 (Nakamura et al., 1995, Plant Physiol. 109:371-4), KST1 (Müller-Röber et al., 1995, EMBO 14:2409-16), Rha1 (Terryn et al., 1993, Plant Cell 5:1761-9; Terryn et al., 1992, FEBS Lett. 299(3):287-90), ARSK1 (Atkinson et al., 1997, GenBank Accession #L22302, and PCT Application No. WO 97/20057), PtxA (Plesch et al., GenBank Accession #X67427), SbHRGP3 (Ahn et al., 1996, Plant Cell 8:1477-90), GH3 (Liu et al., 1994, Plant Cell 6:645-57), the pathogen inducible PRP1-gene promoter (Ward et al., 1993, Plant. Mol. Biol. 22:361-366), the heat inducible hsp80-promoter from tomato (U.S. Pat. No. 5,187,267), cold inducible alpha-amylase promoter from potato (PCT Application No. WO 96/12814), or the wound-inducible pinII-promoter (European Patent No. 375091). For other examples of drought, cold, and salt-inducible promoters, such as the RD29A promoter, see Yamaguchi-Shinozalei at al., 1993, Mol. Gen. Genet, 236:331-340.
  • Developmental stage-preferred promoters are preferentially expressed at certain stages of development. Tissue and organ preferred promoters include those that are preferentially expressed in certain tissues or organs, such as leaves, roots, seeds, or xylem. Examples of tissue-preferred and organ-preferred promoters include, but are not limited to fruit-preferred, ovule-preferred, male tissue-preferred, seed-preferred, integument-preferred, tuber-preferred, stalk-preferred, pericarp-preferred, leaf-preferred, stigma-preferred, pollen-preferred, anther-preferred, petal-preferred, sepal-preferred, pedicel-preferred, silique-preferred, stem-preferred, root-preferred promoters, and the like. Seed-preferred promoters are preferentially expressed during seed development and/or germination. For example, seed-preferred promoters can be embryo-preferred, endosperm-preferred, and seed coat-preferred (See Thompson at al., 1989, BioEssays 10:108). Examples of seed-preferred promoters include, but are not limited to, cellulose synthase (celA), Cim1, gamma-zein, globulin-1, maize 19 kD zein (cZ19B1), and the like.
  • Other suitable tissue-preferred or organ-preferred promoters include the napin-gene promoter from rapeseed (U.S. Pat. No. 5,608,152), the USP-promoter from Vicia faba (Baeumlein et al., 1991, Mol. Gen. Genet. 225(3): 459-67), the oleosin-promoter from Arabidopsis (PCT Application No. WO 98/45461), the phaseolin-promoter from Phaseolus vulgaris (U.S. Pat. No. 5,504,200), the Bce4-promoter from Brassica (PCT Application No. WO 91/13980), or the legumin B4 promoter (LeB4; Baeumlein et al., 1992, Plant Journal, 2(2): 233-9), as well as promoters conferring seed specific expression in monocot plants like maize, barley, wheat, rye, rice, etc. Suitable promoters to note are the lpt2 or lpt1-gene promoter from barley (PCT Application No. WO 95/15389 and PCT Application No. WO 95/23230) or those described in PCT Application No. WO 99/16890 (promoters from the barley hordein-gene, rice glutelin gene, rice oryzin gene, rice prolamin gene, wheat gliadin gene, wheat glutelin gene, oat glutelin gene, Sorghum kasirin-gene, and rye secalin gene).
  • Other promoters useful in the expression cassettes of the invention include, but are not limited to, the major chlorophyll a/b binding protein promoter, histone promoters, the Ap3 promoter, the β-conglycin promoter, the napin promoter, the soybean lectin promoter, the maize 15 kD zein promoter, the 22 kD zein promoter, the 27 kD zein promoter, the g-zein promoter, the waxy, shrunken 1, shrunken 2, and bronze promoters, the Zm13 promoter (U.S. Pat. No. 5,086,169), the maize polygalacturonase promoters (PG) (U.S. Pat. Nos. 5,412,085 and 5,545,546), and the SGB6 promoter (U.S. Pat. No. 5,470,359), as well as synthetic or other natural promoters.
  • Additional flexibility in controlling heterologous gene expression in plants may be obtained by using DNA binding domains and response elements from heterologous sources (i.e., DNA binding domains from non-plant sources). An example of such a heterologous DNA binding domain is the LexA DNA binding domain (Brent and Ptashne, 1985, Cell 43:729-736).
  • In a preferred embodiment of the present invention, the polynucleotides listed in Table 1 are expressed in plant cells from higher plants (e.g., the spermatophytes, such as crop plants). A polynucleotide may be “introduced” into a plant cell by any means, including transfection, transformation or transduction, electroporation, particle bombardment, agroinfection, and the like. Suitable methods for transforming or transfecting plant cells are disclosed, for example, using particle bombardment as set forth in U.S. Pat. Nos. 4,945,050; 5,036,006; 5,100,792; 5,302,523; 5,464,765; 5,120,657; 6,084,154; and the like. More preferably, the transgenic corn seed of the invention may be made using Agrobacterium transformation, as described in U.S. Pat. Nos. 5,591,616; 5,731,179; 5,981,840; 5,990,387; 6,162,965; 6,420,630, U.S. patent application publication number 2002/0104132, and the like. Transformation of soybean can be performed using for example a technique described in European Patent No. EP 0424047, U.S. Pat. No. 5,322,783, European Patent No. EP 0397 687, U.S. Pat. No. 5,376,543, or U.S. Pat. No. 5,169,770. A specific example of wheat transformation can be found in PCT Application No. WO 93/07256. Cotton may be transformed using methods disclosed in U.S. Pat. Nos. 5,004,863; 5,159,135; 5,846,797, and the like. Rice may be transformed using methods disclosed in U.S. Pat. Nos. 4,666,844; 5,350,688; 6,153,813; 6,333,449; 6,288,312; 6,365,807; 6,329,571, and the like. Other plant transformation methods are disclosed, for example, in U.S. Pat. Nos. 5,932,782; 6,153,811; 6,140,553; 5,969,213; 6,020,539, and the like. Any plant transformation method suitable for inserting a transgene into a particular plant may be used in accordance with the invention.
  • According to the present invention, the introduced polynucleotide may be maintained in the plant cell stably if it is incorporated into a non-chromosomal autonomous replicon or integrated into the plant chromosomes. Alternatively, the introduced polynucleotide may be present on an extra-chromosomal non-replicating vector and may be transiently expressed or transiently active.
  • Another aspect of the invention pertains to an isolated polypeptide having a sequence selected from the group consisting of the polypeptide sequences listed in Table 1. An “isolated” or “purified” polypeptide is free of some of the cellular material when produced by recombinant DNA techniques, or chemical precursors or other chemicals when chemically synthesized. The language “substantially free of cellular material” includes preparations of a polypeptide in which the polypeptide is separated from some of the cellular components of the cells in which it is naturally or recombinantly produced. In one embodiment, the language “substantially free of cellular material” includes preparations of a polypeptide of the invention having less than about 30% (by dry weight) of contaminating polypeptides, more preferably less than about 20% of contaminating polypeptides, still more preferably less than about 10% of contaminating polypeptides, and most preferably less than about 5% contaminating polypeptides.
  • The determination of activities and kinetic parameters of enzymes is well established in the art. Experiments to determine the activity of any given altered enzyme must be tailored to the specific activity of the wild-type enzyme, which is well within the ability of one skilled in the art. Overviews about enzymes in general, as well as specific details concerning structure, kinetics, principles, methods, applications and examples for the determination of many enzyme activities are abundant and well known to one skilled in the art.
  • The invention is also embodied in a method of producing a transgenic plant comprising at least one polynucleotide listed in Table 1, wherein expression of the polynucleotide in the plant results in the plant's increased growth and/or yield under normal or water-limited conditions and/or increased tolerance to an environmental stress as compared to a wild type variety of the plant comprising the steps of: (a) introducing into a plant cell an expression vector comprising at least one polynucleotide listed in Table 1, and (b) generating from the plant cell a transgenic plant that expresses the polynucleotide, wherein expression of the polynucleotide in the transgenic plant results in the plant's increased growth and/or yield under normal or water-limited conditions and/or increased tolerance to environmental stress as compared to a wild type variety of the plant. The plant cell may be, but is not limited to, a protoplast, gamete producing cell, and a cell that regenerates into a whole plant. As used herein, the term “transgenic” refers to any plant, plant cell, callus, plant tissue, or plant part, that contains at least one recombinant polynucleotide listed in Table 1. In many cases, the recombinant polynucleotide is stably integrated into a chromosome or stable extra-chromosomal element, so that it is passed on to successive generations.
  • The present invention also provides a method of increasing a plant's growth and/or yield under normal or water-limited conditions and/or increasing a plant's tolerance to an environmental stress comprising the steps of increasing the expression of at least one polynucleotide listed in Table 1 in the plant. Expression of a protein can be increased by any method known to those of skill in the art.
  • The effect of the genetic modification on plant growth and/or yield and/or stress tolerance can be assessed by growing the modified plant under normal and.or less than suitable conditions and then analyzing the growth characteristics and/or metabolism of the plant. Such analysis techniques are well known to one skilled in the art, and include dry weight, wet weight, polypeptide synthesis, carbohydrate synthesis, lipid synthesis, evapotranspiration rates, general plant and/or crop yield, flowering, reproduction, seed setting, root growth, respiration rates, photosynthesis rates, metabolite composition, etc., using methods known to those of skill in biotechnology.
  • The invention is further illustrated by the following examples, which are not to be construed in any way as imposing limitations upon the scope thereof.
    • Example 1 Cloning of cDNAs
  • cDNAs were isolated from proprietary libraries of the respective plant species using known methods. Sequences were processed and annotated using bioinformatics analyses. The degrees of amino acid identity and similarity of the isolated sequences to the respective closest known public sequences are indicated in Tables 2 through 18 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).
  • TABLE 2
    Comparison of At2g20725 (SEQ ID NO: 2) to
    known CAAX amino terminal protease proteins
    Public Database Sequence
    Accession # Species Identity (%)
    NP_565483 A. thaliana 99.70%
    ABE87113 Medicago truncatula 27.00%
    NP_563943 A. thaliana 25.60%
    AAU90306 Solanum tuberosum 25.20%
    AAM65055 A. thaliana 25.00%
  • TABLE 3
    Comparison of At3g26085 (SEQ ID NO: 4) to
    known CAAX amino terminal protease proteins
    Public Database Sequence
    Accession # Species Identity (%)
    NP_566788 A. thaliana 100.00%
    BAC43478 A. thaliana 99.70%
    AAM63917 A. thaliana 99.30%
    NP_001078210 A. thaliana 91.00%
    BAB01072 A. thaliana 65.30%
  • TABLE 4
    Comparison of AtFACE-2 (SEQ ID NO: 6) to
    known prenyl-dependent CAAX proteases
    Public Database Sequence
    Accession # Species Identity (%)
    NP_850262 A. thaliana 100.00%
    BAC43705 A. thaliana 99.70%
    CAN61196 Vitis vinifera 36.70%
    XP_695285 Danio rerio 32.70%
    XP_001342272 D. rerio 32.70%
  • TABLE 5
    Comparison of CASAR82A (SEQ ID NO: 12)
    to known SAR8.2 protein precursors
    Public Database Sequence
    Accession # Species Identity (%)
    AAF18935 C. annuum 100.00%
    AAL56986 C. annuum 97.70%
    AAL16783 C. annuum 93.00%
    AAL16782 C. annuum 91.90%
    AAR97871 C. annuum 52.30%
  • TABLE 6
    Comparison of b3358 (SEQ ID NO: 14)
    to known putative membrane proteins
    Public Database Sequence
    Accession # Species Identity (%)
    YP_312284 Shigella sonnei 99.90%
    ZP_00715046 E. coli 99.90%
    ZP_00725390 E. coli 99.60%
    AP_004431 E. coli 99.40%
    YP_858957 E. coli 99.40%
  • TABLE 7
    Comparison of EST564 (SEQ ID NO: 16) to
    known protein phosphatase 2C proteins
    Public Database Sequence
    Accession # Species Identity (%)
    ABF93864 O. sativa 56.40%
    NP_974411 A. thaliana 51.60%
    AAC35951 Mesembryanthemum 51.10%
    crystallinum
    EAZ25504 O. sativa 45.70%
    EAZ02383 O. sativa 43.40%
  • TABLE 8
    Comparison of EST390 (SEQ ID NO: 36) to
    known mitochondrial carrier proteins
    Public Database Sequence
    Accession # Species Identity (%)
    NP_172866 A. thaliana 63.50%
    AAT66766 Solanum demissum 60.80%
    CAH67091 O. sativa 60.00%
    CAE01569 O. sativa 59.70%
    CAN75338 V. vinifera 59.50%
  • TABLE 9
    Comparison of EST257 (SEQ ID NO: 50) to known protein kinases
    Public Database Sequence
    Accession # Species Identity (%)
    NP_001043682 O. sativa 62.20%
    CAN82019 V. vinifera 62.10%
    AAR01726 O. sativa 61.10%
    NP_001056408 O. sativa 61.10%
    CAN64754 V. vinifera 60.90%
  • TABLE 10
    Comparison of EST465 (SEQ ID NO: 62)
    to known peptidyl prolyl isomerases
    Public Database Sequence
    Accession # Species Identity (%)
    AAC39445 A. thaliana 54.30%
    ABE85899 M. truncatula 54.20%
    CAB88363 A. thaliana 54.10%
    NP_566993 A. thaliana 53.80%
    NP_001050182 O. sativa 53.00%
  • TABLE 11
    Comparison of YBL109w (SEQ ID NO: 64) to unknown protein 1
    Public Database Sequence
    Accession # Species Identity (%)
    CAA84936 S. cerevisiae 49.50%
    P38898 S. cerevisiae 43.10%
  • TABLE 12
    Comparison of YBL100c (SEQ ID NO: 66) to unknown protein 2
    Public Database Sequence
    Accession # Species Identity (%)
    P38168 S. cerevisiae 100.00%
  • TABLE 13
    Comparison of YKL184w (SEQ ID NO: 68)
    to known ornithine decarboxylases
    Public Database Sequence
    Accession # Species Identity (%)
    NP_012737 S. cerevisiae 100.00%
    XP_445434 Candida glabrata 70.90%
    XP_451651 Kluyveromyces lactis 60.30%
    NP_984947 Ashbya gossypii 57.40%
    XP_001385782 P. stipitis 49.80%
  • TABLE 14
    Comparison of YPL091w (SEQ ID NO:
    70) to known glutathione reductases
    Public Database Sequence
    Accession # Species Identity (%)
    NP_015234 S. cerevisiae 100.00%
    AAA92575 S. cerevisiae 96.70%
    BAA07109 S. cerevisiae 95.70%
    XP_447042 C. glabrata 79.90%
    XP_455036 K. lactis 73.30%
  • TABLE 15
    Comparison of TA54587433 (SEQ
    ID NO: 72) to unknown protein 3
    Public Database Sequence
    Accession # Species Identity (%)
    EAY88696 O. sativa 22.80%
    EAZ25723 O. sativa 21.90%
    NP_001049087 O. sativa 21.20%
  • TABLE 16
    Comparison of ZM68532504 (SEQ ID NO: 74)
    to known protein phosphatase 2A proteins
    Public Database Sequence
    Accession # Species Identity (%)
    AAC72838 O. sativa 95.40%
    AAA91806 O. sativa 94.10%
    BAA92697 Vicia faba 93.10%
    AAQ67226 Lycopersicon esculentum 92.80%
    BAD17175 O. sativa 92.80%
  • TABLE 17
    Comparison of ZM59202533 (SEQ ID NO:
    92) to known MEK1 protein kinases
    Public Database Sequence
    Accession # Species Identity (%)
    AAC83393 Z. mays 100.00%
    ABG45894 O. sativa 92.70%
    NP_001043164 O. sativa 85.90%
    BAB32405 Nicotiana tabacum 77.80%
    CAC24705 N. tabacum 77.20%
  • TABLE 18
    Comparison of BN42671700 (SEQ ID NO: 98) to known
    AP2 domain containing transcription factors
    Public Database Sequence
    Accession # Species Identity (%)
    NP_177631 A. thaliana 58.60%
    NP_173355 A. thaliana 56.70%
    AAF82238 A. thaliana 54.80%
  • The full-length DNA sequence of the AtFACE-2 (SEQ ID NO: 5) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e−10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Two homologs from maize were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 19 and 20 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix; blosum62).
  • TABLE 19
    Comparison of ZM57353913 (SEQ ID NO: 8)
    to known prenyl-dependent CAAX proteases
    Public Database Sequence
    Accession # Species Identity (%)
    NP_850262 A. thaliana 52.20%
    BAC43705 A. thaliana 52.20%
    NP_001055298 O. sativa 42.10%
    EAZ33973 O. sativa 36.60%
    XP_001353747 Drosophila pseudoobscura 33.50%
  • TABLE 20
    Comparison of ZM59252659 (SEQ ID NO: 10)
    to known prenyl-dependent CAAX proteases
    Public Database Sequence
    Accession # Species Identity (%)
    NP_850262 A. thaliana 47.00%
    BAC43705 A. thaliana 47.00%
    EAZ33973 O. sativa 41.10%
    NP_001055298 O. sativa 38.30%
    CAN61196 V. vinifera 31.90%
  • The full-length DNA sequence of EST564 (SEQ ID NO: 15) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e−10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Six homologs from maize, two homologs from soybean, and one homolog from canda were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 21-29 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).
  • TABLE 21
    Comparison of BN49502266 (SEQ ID NO: 18)
    to known protein phosphatase 2C proteins
    Public Database Sequence
    Accession # Species Identity (%)
    NP_195118 A. thaliana 91.10%
    NP_001067133 O. sativa 63.20%
    EAY83661 O. sativa 60.80%
    EAZ21008 O. sativa 60.50%
    CAN76780 V. vinifera 57.60%
  • TABLE 22
    Comparison of GM49788080 (SEQ ID NO: 20)
    to known protein phosphatase 2C proteins
    Public Database Sequence
    Accession # Species Identity (%)
    EAZ02383 O. sativa 75.60%
    EAZ38299 O. sativa 75.30%
    CAB90634 Fagus sylvatica 73.80%
    EAZ25504 O. sativa 73.00%
    AAC35951 M. crystallinum 72.80%
  • TABLE 23
    Comparison of GM53049821 (SEQ ID NO: 22)
    to known protein phosphatase 2C proteins
    Public Database Sequence
    Accession # Species Identity (%)
    CAN72598 V. vinifera 82.40%
    NP_566566 A. thaliana 73.50%
    AAM61747 A. thaliana 73.50%
    BAA94987 A. thaliana 73.00%
    NP_001051801 O. sativa 60.20%
  • TABLE 24
    Comparison of ZM58462719 (SEQ ID NO: 24)
    to known protein phosphatase 2C proteins
    Public Database Sequence
    Accession # Species Identity (%)
    NP_001058597 O. sativa 91.10%
    EAZ02383 O. sativa 81.20%
    EAZ38299 O. sativa 81.00%
    AAD11430 M. crystallinum 75.70%
    CAB90634 F. sylvatica 74.20%
  • TABLE 25
    Comparison of ZM61092633 (SEQ ID NO: 26)
    to known protein phosphatase 2C proteins
    Public Database Sequence
    Accession # Species Identity (%)
    NP_001065203 O. sativa 87.00%
    AAK20060 O. sativa 86.00%
    NP_001048899 O. sativa 80.70%
    EAY88457 O. sativa 79.90%
    ABE77197 Sorghum bicolor 77.20%
  • TABLE 26
    Comparison of ZM62016485 (SEQ ID NO: 28)
    to known protein phosphatase 2C proteins
    Public Database Sequence
    Accession # Species Identity (%)
    ABE77197 S. bicolor 90.70%
    NP_001048899 O. sativa 86.20%
    EAY88457 O. sativa 85.20%
    NP_001065203 O. sativa 78.50%
    AAK20060 O. sativa 77.80%
  • TABLE 27
    Comparison of ZM62051019 (SEQ ID NO: 30)
    to known protein phosphatase 2C proteins
    Public Database Sequence
    Accession # Species Identity (%)
    ABE77197 S. bicolor 92.50%
    NP_001048899 O. sativa 88.00%
    EAY88457 O. sativa 87.00%
    NP_001065203 O. sativa 79.50%
    AAK20060 O. sativa 78.80%
  • TABLE 28
    Comparison of ZM65086957 (SEQ ID NO: 32)
    to known protein phosphatase 2C proteins
    Public Database Sequence
    Accession # Species Identity (%)
    ABE77197 S. bicolor 91.00%
    NP_001048899 O. sativa 86.50%
    EAY88457 O. sativa 85.50%
    NP_001065203 O. sativa 78.80%
    AAK20060 O. sativa 78.00%
  • TABLE 29
    Comparison of ZM68587657 (SEQ ID NO: 34)
    to known protein phosphatase 2C proteins
    Public Database Sequence
    Accession # Species Identity (%)
    EAZ02383 O. sativa 70.60%
    EAZ38299 O. sativa 70.60%
    AAC35951 M. crystallinum 69.80%
    ABF93864 O. sativa 68.50%
    NP_974411 A. thaliana 65.00%
  • The full-length DNA sequence of the EST390 (SEQ ID NO: 35) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e−10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Four homologs from canola and two homologs from maize were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 30-35 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum82).
  • TABLE 30
    Comparison of BN51363030 (SEQ ID NO: 38)
    to known mitochondrial carrier proteins
    Public Database Sequence
    Accession # Species Identity (%)
    CAN77545 V. vinifera 71.90%
    BAE71294 Trifolium pratense 71.90%
    NP_194188 A. thaliana 70.70%
    AAU11466 Saccharum officinarum 70.60%
    AAU11465 S. officinarum 69.90%
  • TABLE 31
    Comparison of BN42986056 (SEQ ID NO: 40)
    to known mitochondrial carrier proteins
    Public Database Sequence
    Accession # Species Identity (%)
    NP_179836 A. thaliana 74.80%
    AAK44155 A. thaliana 74.50%
    AAM63236 A. thaliana 74.20%
    CAN77545 V. vinifera 67.70%
    BAE71294 Trifolium pratense 65.50%
  • TABLE 32
    Comparison of BN49389066 (SEQ ID NO: 42)
    to known mitochondrial carrier proteins
    Public Database Sequence
    Accession # Species Identity (%)
    AAY97866 L. esculentum 73.50%
    CAA68164 Solanum tuberosum 73.50%
    CAC84547 N. tabacum 73.30%
    AAR06239 Citrus junos 73.00%
    CAC84545 N. tabacum 73.00%
  • TABLE 33
    Comparison of BN51339479 (SEQ ID NO: 44)
    to known mitochondrial carrier proteins
    Public Database Sequence
    Accession # Species Identity (%)
    CAC84545 N. tabacum 85.60%
    CAC84547 N. tabacum 85.30%
    AAR06239 C. junos 85.30%
    CAA68164 S. tuberosum 85.30%
    CAC12820 N. tabacum 85.30%
  • TABLE 34
    Comparison of ZM57651070 (SEQ ID NO: 46)
    to known mitochondrial carrier proteins
    Public Database Sequence
    Accession # Species Identity (%)
    NP_001066927 O. sativa 57.00%
    NP_680566 A. thaliana 53.80%
    BAF00711 A. thaliana 51.70%
    CAN71674 V. vinifera 43.20%
    CAN71674 V. vinifera 43.20%
  • TABLE 35
    Comparison of ZM62073276 (SEQ ID NO: 48)
    to known mitochondrial carrier proteins
    Public Database Sequence
    Accession # Species Identity (%)
    AAU11471 S. officinarum 94.90%
    NP_001054904 O. sativa 92.30%
    BAA08105 Panicum miliaceum 86.20%
    BAA08103 P. miliaceum 85.50%
    EAY80779 O. sativa 82.90%
  • The full-length DNA sequence of the EST257 (SEQ ID NO: 49) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e−10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Three homologs from maize, one homolog from linseed, and one sequence from wheat were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 36-40 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).
  • TABLE 36
    Comparison of LU61665952 (SEQ ID
    NO: 52) to known protein kinases
    Public Database Sequence
    Accession # Species Identity (%)
    NP_566716 A. thaliana 75.10%
    CAN82019 V. vinifera 74.50%
    NP_193214 A. thaliana 74.50%
    ABK06452 synthetic construct 73.00%
    ABK06453 synthetic construct 72.30%
  • TABLE 37
    Comparison of TA56863186 (SEQ ID
    NO: 54) to known protein kinases
    Public Database Sequence
    Accession # Species Identity (%)
    AAO72550 O. sativa 87.30%
    NP_001046047 O. sativa 79.80%
    EAZ01979 O. sativa 73.80%
    NP_001058291 O. sativa 73.60%
    AAO48744 O. sativa 73.40%
  • TABLE 38
    Comparison of ZM62026837 (SEQ ID
    NO: 56) to known protein kinases
    Public Database Sequence
    Accession # Species Identity (%)
    AAR01726 O. sativa 83.40%
    NP_001050732 O. sativa 77.00%
    EAY91142 O. sativa 76.30%
    EAZ27891 O. sativa 76.00%
    CAN82019 V. vinifera 73.30%
  • TABLE 39
    Comparison of ZM65457595 (SEQ ID
    NO: 58) to known protein kinases
    Public Database Sequence
    Accession # Species Identity (%)
    NP_001056408 O. sativa 89.60%
    AAO72572 O. sativa 87.20%
    NP_001043682 O. sativa 81.50%
    CAN64754 V. vinifera 79.80%
    NP_199811 A. thaliana 77.20%
  • TABLE 40
    Comparison of ZM67230154 (SEQ ID
    NO: 60) to known protein kinases
    Public Database Sequence
    Accession # Species Identity (%)
    NP_001043682 O. sativa 87.10%
    NP_001056408 O. sativa 82.80%
    AAO72572 O. sativa 80.80%
    EAZ12861 O. sativa 79.20%
    CAN64754 V. vinifera 77.50%
  • The full-length DNA sequence of the ZM68532504 (SEQ ID NO: 73) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e−10 (Altschul at al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Two homologs from canola, two homologs from maize, one homolog from linseed, two sequences from rice and one sequence from sunflower were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 41-48 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).
  • TABLE 41
    Comparison of BN42856089 (SEQ ID NO: 76)
    to known protein phosphatase 2A proteins
    Public Database Sequence
    Accession # Species Identity (%)
    NP_172514 A. thaliana 97.10%
    AAM65099 A. thaliana 95.80%
    AAQ67226 L. esculentum 95.40%
    BAA92697 Vicia faba 95.10%
    CAC11129 Fagus sylvatica 94.40%
  • TABLE 42
    Comparison of BN43206527 (SEQ ID NO: 78)
    to known protein phosphatase 2A proteins
    Public Database Sequence
    Accession # Species Identity (%)
    NP_172514 A. thaliana 97.40%
    AAM65099 A. thaliana 96.10%
    AAQ67226 L. esculentum 95.10%
    BAA92697 V. faba 94.10%
    AAQ67225 L. esculentum 94.10%
  • TABLE 43
    Comparison of HA66872964 (SEQ ID NO: 80)
    to known protein phosphatase 2A proteins
    Public Database Sequence
    Accession # Species Identity (%)
    P48579 H. annuus 99.30%
    BAA92697 V. faba 93.50%
    CAC11129 F. sylvatica 93.10%
    BAA92698 V. faba 92.80%
    Q9ZSE4 Hevea brasiliensis 92.80%
  • TABLE 44
    Comparison of LU61662612 (SEQ ID NO: 82)
    to known protein phosphatase 2A proteins
    Public Database Sequence
    Accession # Species Identity (%)
    AAQ67226 L. esculentum 94.10%
    BAA92697 V. faba 94.10%
    BAA92698 V. faba 94.10%
    CAN74947 V. vinifera 93.50%
    CAC11129 F. sylvatica 93.10%
  • TABLE 45
    Comparison of OS32806943 (SEQ ID NO: 84)
    to known protein phosphatase 2A proteins
    Public Database Sequence
    Accession # Species Identity (%)
    AAC72838 O. sativa 96.10%
    BAD17175 O. sativa 95.80%
    AAA91806 O. sativa 94.80%
    AAQ67226 L. esculentum 93.20%
    BAA92697 V. faba 93.20%
  • TABLE 46
    Comparison of OS34738749 (SEQ ID NO: 86)
    to known protein phosphatase 2A proteins
    Public Database Sequence
    Accession # Species Identity (%)
    AAQ67226 L. esculentum 97.70%
    BAA92697 V. faba 97.10%
    CAC11129 F. sylvatica 96.70%
    BAA92698 V. faba 96.10%
    AAQ67225 L. esculentum 96.10%
  • TABLE 47
    Comparison of ZM59400933 (SEQ ID NO: 88)
    to known protein phosphatase 2A proteins
    Public Database Sequence
    Accession # Species Identity (%)
    AAC72838 O. sativa 95.80%
    AAA91806 O. sativa 94.40%
    BAA92697 V. faba 92.80%
    AAQ67226 L. esculentum 92.80%
    AAQ67225 L. esculentum 92.80%
  • TABLE 48
    Comparison of ZM62132060 (SEQ ID NO: 90)
    to known protein phosphatase 2A proteins
    Public Database Sequence
    Accession # Species Identity (%)
    AAC72838 O. sativa 95.10%
    AAA91806 O. sativa 93.80%
    BAA92697 V. faba 92.80%
    AAQ67226 L. esculentum 92.50%
    BAD17175 O. sativa 92.50%
  • The full-length DNA sequence of the ZM59202533 (SEQ ID NO: 91) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e−10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Two homologs from canola and one homolog from maize were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 49-51 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).
  • TABLE 49
    Comparison of BN41901422 (SEQ ID NO:
    94) to known MEK1 protein kinases
    Public Database Sequence
    Accession # Species Identity (%)
    ABF55661 synthetic construct 79.80%
    NP_849446 A. thaliana 76.30%
    AAQ96337 Vitis aestivalis 66.00%
    AAL62336 G. max 64.10%
    AAS21304 Petroselinum crispum 63.60%
  • TABLE 50
    Comparison of BN47868329 (SEQ ID NO:
    96) to known MEK1 protein kinases
    Public Database Sequence
    Accession # Species Identity (%)
    NP_188759 A. thaliana 72.30%
    CAA68958 A. thaliana 72.00%
    ABF55664 synthetic construct 70.90%
    AAL91161 A. thaliana 70.10%
    AAU04434 L. esculentum 66.40%
  • TABLE 51
    Comparison of ZM68416988 (SEQ ID NO:
    100) to known MEK1 protein kinases
    Public Database Sequence
    Accession # Species Identity (%)
    ABI93775 Oryza minuta 80.00%
    NP_001056806 O. sativa 79.70%
    ABP88102 O. sativa 78.90%
    CAD45180 O. sativa 75.20%
    ABI93776 O. minuta 72.40%
    • Example 2 Well-Watered Arabidopsis Plants
  • The polynucleotides of Table 1 are ligated into a binary vector containing a selectable marker. The resulting recombinant vector contains the corresponding gene in the sense orientation under a constitutive promoter. The recombinant vectors are transformed into an Agrobacterium tumefaciens strain according to standard conditions. A. thaliana ecotype Col-0 or C24 are grown and transformed according to standard conditions. T1 and T2 plants are screened for resistance to the selection agent conferred by the selectable marker gene. T3 seeds are used in greenhouse or growth chamber experiments.
  • Approximately 3-5 days prior to planting, seeds are refrigerated for stratification. Seeds are then planted, fertilizer is applied and humidity is maintained using transparent domes. Plants are grown in a greenhouse at 22° C. with photoperiod of 16 hours light/8 hours dark. Plants are watered twice a week.
  • At 19 and 22 days, plant area, leaf area, biomass, color distribution, color intensity, and growth rate for each plant are measured using a commercially available imaging system. Biomass is calculated as the total plant leaf area at the last measuring time point. Growth rate is calculated as the plant leaf area at the last measuring time point minus the plant leaf area at the first measuring time point divided by the plant leaf area at the first measuring time point. Health index is calculated as the dark green leaf area divided by the total plant leaf area.
    • Example 3 Nitrogen Stress Tolerant Arabidopsis Plants
  • The polynucleotides of Table 1 are ligated into a binary vector containing a selectable marker. The resulting recombinant vector contains the corresponding gene in the sense orientation under a constitutive promoter. The recombinant vectors are transformed into an A. tumefaciens strain according to standard conditions. A. thaliana ecotype Col-0 or C24 are grown and transformed according to standard conditions. T1 and T2 plants are screened for resistance to the selection agent conferred by the selectable marker gene.
  • Plants are grown in flats using a substrate that contains no organic components. Each flat is wet with water before seedlings resistant to the selection agent are transplanted onto substrate. Plants are grown in a growth chamber set to 22° C. with a 55% relative humidity with photoperiod set at 16 h light/8 h dark. A controlled low or high nitrogen nutrient solution is added to waterings on Days 12, 15, 22 and 29. Watering without nutrient solution occurs on Days 18, 25, and 32. Images of all plants in a tray are taken on days 26, 30, and 33 using a commercially available imaging system. At each imaging time point, biomass and plant phenotypes for each plant are measured including plant area, leaf area, biomass, color distribution, color intensity, and growth rate.
    • Example 4 Stress-Tolerant Rapeseed/Canola Plants
  • Canola cotyledonary petioles of 4 day-old young seedlings are used as explants for tissue culture and transformed according to EP1566443. The commercial cultivar Westar (Agriculture Canada) is the standard variety used for transformation, but other varieties can be used. A. tumefaciens GV3101:pMP90RK containing a binary vector is used for canola transformation. The standard binary vector used for transformation is pSUN (WO02/00900), but many different binary vector systems have been described for plant transformation (e.g. An, G. in Agrobacterium Protocols, Methods in Molecular Biology vol 44, pp 47-62, Gartland K M A and M R Davey eds. Humana Press, Totowa, N.J.). A plant gene expression cassette comprising a selection marker gene, a plant promoter, and a polynucleotide of Table 1 is employed. Various selection marker genes can be used including the mutated acetohydroxy acid synthase (AHAS) gene disclosed in U.S. Pat. Nos. 5,767,366 and 6,225,105. A suitable promoter is used to regulate the trait gene to provide constitutive, developmental, tissue or environmental regulation of gene transcription.
  • Canola seeds are surface-sterilized in 70% ethanol for 2 min, incubated for 15 min in 55° C. warm tap water and then in 1.5% sodium hypochlorite for 10 minutes, followed by three rinses with sterilized distilled water. Seeds are then placed on MS medium without hormones, containing Gamborg B5 vitamins, 3% sucrose, and 0.8% Oxoidagar. Seeds are germinated at 24° C. for 4 days in low light (<50 μMol/m2s, 16 hours light). The cotyledon petiole explants with the cotyledon attached are excised from the in vitro seedlings, and inoculated with Agrobacterium by dipping the cut end of the petiole explant into the bacterial suspension. The explants are then cultured for 3 days on MS medium including vitamins containing 3.75 mg/l BAP, 3% sucrose, 0.5 g/l MES, pH 5.2, 0.5 mg/l GA3, 0.8% Oxoidagar at 24° C., 16 hours of light. After three days of co-cultivation with Agrobacterium, the petiole explants are transferred to regeneration medium containing 3.75 mg/l BAP, 0.5 mg/l GA3, 0.5 g/l MES, pH 5.2, 300 mg/l timentin and selection agent until shoot regeneration. As soon as explants start to develop shoots, they are transferred to shoot elongation medium (A6, containing full strength MS medium including vitamins, 2% sucrose, 0.5% Oxoidagar, 100 mg/l myo-inositol, 40 mg/l adenine sulfate, 0.5 WI MES, pH 5.8, 0.0025 mg/l BAP, 0.1 mg/l IBA, 300 mg/l timentin and selection agent).
  • Samples from both in vitro and greenhouse material of the primary transgenic plants (T0) are analyzed by qPCR using TaqMan probes to confirm the presence of T-DNA and to determine the number of T-DNA integrations.
  • Seed is produced from the primary transgenic plants by self-pollination. The second-generation plants are grown in greenhouse conditions and self-pollinated. The plants are analyzed by qPCR using TaqMan probes to confirm the presence of T-DNA and to determine the number of T-DNA integrations. Homozygous transgenic, heterozygous transgenic and azygous (null transgenic) plants are compared for their stress tolerance, for example, in the assays described in Examples 2 and 3, and for yield, both in the greenhouse and in field studies.
    • Example 5 Screening for Stress-Tolerant Rice Plants
  • Transgenic rice plants comprising a polynucleotide of Table 1 are generated using known methods. Approximately 15 to 20 independent transformants (T0) are generated. The primary transformants are transferred from tissue culture chambers to a greenhouse for growing and harvest of T1 seeds. Five events of the T1 progeny segregated 3:1 for presence/absence of the transgene are retained. For each of these events, 10 T1 seedlings containing the transgene (hetero- and homozygotes), and 10 T1 seedlings lacking the transgene (nullizygotes) are selected by visual marker screening. The selected T1 plants are transferred to a greenhouse. Each plant receives a unique barcode label to link unambiguously the phenotyping data to the corresponding plant. The selected T1 plants are grown on soil in 10 cm diameter pots under the following environmental settings: photoperiod=11.5 h, daylight intensity=30,000 lux or more, daytime temperature=28° C. or higher, night time temperature=22° C., relative humidity=60-70%. Transgenic plants and the corresponding nullizygotes are grown side-by-side at random positions. From the stage of sowing until the stage of maturity, the plants are passed several times through a digital imaging cabinet. At each time point digital, images (2048×1536 pixels, 16 million colours) of each plant are taken from at least 6 different angles.
  • The data obtained in the first experiment with T1 plants are confirmed in a second experiment with T2 plants. Lines that have the correct expression pattern are selected for further analysis. Seed batches from the positive plants (both hetero- and homozygotes) in T1 are screened by monitoring marker expression. For each chosen event, the heterozygote seed batches are then retained for T2 evaluation. Within each seed batch, an equal number of positive and negative plants are grown in the greenhouse for evaluation.
  • Transgenic plants are screened for their improved growth and/or yield and/or stress tolerance, for example, using the assays described in Examples 2 and 3, and for yield, both in the greenhouse and in field studies.
    • Example 6 Stress-Tolerant Soybean Plants
  • The polynucleotides of Table 1 are transformed into soybean using the methods described in commonly owned copending international application number WO 2005/121345, the contents of which are incorporated herein by reference.
  • The transgenic plants generated are then screened for their improved growth under water-limited conditions and/or drought, salt, and/or cold tolerance, for example, using the assays described in Examples 2 and 3, and for yield, both in the greenhouse and in field studies.
    • Example 7 Stress-Tolerant Wheat Plants
  • The polynucleotides of Table 1 are transformed into wheat using the method described by Ishida et al., 1996, Nature Biotech. 14745-50. Immature embryos are co-cultivated with Agrobacterium tumefaciens that carry “super binary” vectors, and transgenic plants are recovered through organogenesis. This procedure provides a transformation efficiency between 2.5% and 20%. The transgenic plants are then screened for their improved growth and/or yield under water-limited conditions and/or stress tolerance, for example, is the assays described in Examples 2 and 3, and for yield, both in the greenhouse and in field studies.
    • Example 8 Stress-Tolerant Corn Plants
  • The polynucleotides of Table 1 are transformed into immature embryos of corn using Agrobacterium. After imbibition, embryos are transferred to medium without selection agent. Seven to ten days later, embryos are transferred to medium containing selection agent and grown for 4 weeks (two 2-week transfers) to obtain transformed callus cells. Plant regeneration is initiated by transferring resistant calli to medium supplemented with selection agent and grown under light at 25-27° C. for two to three weeks. Regenerated shoots are then transferred to rooting box with medium containing selection agent. Plantlets with roots are transferred to potting mixture in small pots in the greenhouse and after acclimatization are then transplanted to larger pots and maintained in greenhouse till maturity.
  • Using assays such as the assay described in Examples 2 and 3, each of these plants is uniquely labeled, sampled and analyzed for transgene copy number. Transgene positive and negative plants are marked and paired with similar sizes for transplanting together to large pots. This provides a uniform and competitive environment for the transgene positive and negative plants. The large pots are watered to a certain percentage of the field water capacity of the soil depending the severity of water-stress desired. The soil water level is maintained by watering every other day. Plant growth and physiology traits such as height, stem diameter, leaf rolling, plant wilting, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured during the growth period. After a period of growth, the above ground portion of the plants is harvested, and the fresh weight and dry weight of each plant are taken. A comparison of the drought tolerance phenotype between the transgene positive and negative plants is then made.
  • Using assays such as the assay described in Example 2 and 3, the pots are covered with caps that permit the seedlings to grow through but minimize water loss. Each pot is weighed periodically and water added to maintain the initial water content. At the end of the experiment, the fresh and dry weight of each plant is measured, the water consumed by each plant is calculated and WUE of each plant is computed. Plant growth and physiology traits such as WUE, height, stem diameter, leaf rolling, plant wilting, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured during the experiment. A comparison of WUE phenotype between the transgene positive and negative plants is then made.
  • Using assays such as the assay described in Example 2 and 3, these pots are kept in an area in the greenhouse that has uniform environmental conditions, and cultivated optimally. Each of these plants is uniquely labeled, sampled and analyzed for transgene copy number. The plants are allowed to grow under theses conditions until they reach a predefined growth stage. Water is then withheld. Plant growth and physiology traits such as height, stem diameter, leaf rolling, plant wilting, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured as stress intensity increases. A comparison of the dessication tolerance phenotype between transgene positive and negative plants is then made.
  • Segregating transgenic corn seeds for a transformation event are planted in small pots for testing in a cycling drought assay. These pots are kept in an area in the greenhouse that has uniform environmental conditions, and cultivated optimally. Each of these plants is uniquely labeled, sampled and analyzed for transgene copy number. The plants are allowed to grow under theses conditions until they reach a predefined growth stage. Plants are then repeatedly watered to saturation at a fixed interval of time. This water/drought cycle is repeated for the duration of the experiment. Plant growth and physiology traits such as height, stem diameter, leaf rolling, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured during the growth period. At the end of the experiment, the plants are harvested for above-ground fresh and dry weight. A comparison of the cycling drought tolerance phenotype between transgene positive and negative plants is then made.
  • In order to test segregating transgenic corn for drought tolerance under rain-free conditions, managed-drought stress at a single location or multiple locations is used. Crop water availability is controlled by drip tape or overhead irrigation at a location which has less than 10 cm rainfall and minimum temperatures greater than 5° C. expected during an average 5 month season, or a location with expected in-season precipitation intercepted by an automated “rain-out shelter” which retracts to provide open field conditions when not required. Standard agronomic practices in the area are followed for soil preparation, planting, fertilization and pest control. Each plot is sown with seed segregating for the presence of a single transgenic insertion event. A Taqman transgene copy number assay is used on leaf samples to differentiate the transgenics from null-segregant control plants. Plants that have been genotyped in this manner are also scored for a range of phenotypes related to drought-tolerance, growth and yield. These phenotypes include plant height, grain weight per plant, grain number per plant, ear number per plant, above ground dry-weight, leaf conductance to water vapor, leaf CO2 uptake, leaf chlorophyll content, photosynthesis-related chlorophyll fluorescence parameters, water use efficiency, leaf water potential, leaf relative water content, stem sap flow rate, stem hydraulic conductivity, leaf temperature, leaf reflectance, leaf light absorptance, leaf area, days to flowering, anthesis-silking interval, duration of grain fill, osmotic potential, osmotic adjustment, root size, leaf extension rate, leaf angle, leaf rolling and survival. All measurements are made with commercially available instrumentation for field physiology, using the standard protocols provided by the manufacturers. Individual plants are used as the replicate unit per event.
  • In order to test non-segregating transgenic corn for drought tolerance under rain-free conditions, managed-drought stress at a single location or multiple locations is used. Crop water availability is controlled by drip tape or overhead irrigation at a location which has less than 10 cm rainfall and minimum temperatures greater than 5° C. expected during an average 5 month season, or a location with expected in-season precipitation intercepted by an automated “rain-out shelter” which retracts to provide open field conditions when not required. Standard agronomic practices in the area are followed for soil preparation, planting, fertilization and pest control. Trial layout is designed to pair a plot containing a non-segregating transgenic event with an adjacent plot of null-segregant controls. A null segregant is progeny (or lines derived from the progeny) of a transgenic plant that does not contain the transgene due to Mendelian segregation. Additional replicated paired plots for a particular event are distributed around the trial. A range of phenotypes related to drought-tolerance, growth and yield are scored in the paired plots and estimated at the plot level. When the measurement technique could only be applied to individual plants, these are selected at random each time from within the plot. These phenotypes include plant height, grain weight per plant, grain number per plant, ear number per plant, above ground dry-weight, leaf conductance to water vapor, leaf CO2 uptake, leaf chlorophyll content, photosynthesis-related chlorophyll fluorescence parameters, water use efficiency, leaf water potential, leaf relative water content, stem sap flow rate, stem hydraulic conductivity, leaf temperature, leaf reflectance, leaf light absorptance, leaf area, days to flowering, anthesis-silking interval, duration of grain fill, osmotic potential, osmotic adjustment, root size, leaf extension rate, leaf angle, leaf rolling and survival. All measurements are made with commercially available instrumentation for field physiology, using the standard protocols provided by the manufacturers. Individual plots are used as the replicate unit per event.
  • To perform multi-location testing of transgenic corn for drought tolerance and yield, five to twenty locations encompassing major corn growing regions are selected. These are widely distributed to provide a range of expected crop water availabilities based on average temperature, humidity, precipitation and soil type. Crop water availability is not modified beyond standard agronomic practices. Trial layout is designed to pair a plot containing a non-segregating transgenic event with an adjacent plot of null-segregant controls. A range of phenotypes related to drought-tolerance, growth and yield are scored in the paired plots and estimated at the plot level. When the measurement technique could only be applied to individual plants, these are selected at random each time from within the plot. These phenotypes included plant height, grain weight per plant, grain number per plant, ear number per plant, above ground dry-weight, leaf conductance to water vapor, leaf CO2 uptake, leaf chlorophyll content, photosynthesis-related chlorophyll fluorescence parameters, water use efficiency, leaf water potential, leaf relative water content, stem sap flow rate, stem hydraulic conductivity, leaf temperature, leaf reflectance, leaf light absorptance, leaf area, days to flowering, anthesis-silking interval, duration of grain fill, osmotic potential, osmotic adjustment, root size, leaf extension rate, leaf angle, leaf rolling and survival. All measurements are made with commercially available instrumentation for field physiology, using the standard protocols provided by the manufacturers. Individual plots are used as the replicate unit per event.
  • APPENDIX
    cDNA sequence of At2g20725 from Arabidopsis (SEQ ID NO: 1):
    ATGATTCTAGGCCGATGGGTTTCCTTCAGTTGCGGAAACACGCCGGTGACTAATTGTT
    CCGAACGACGACGACATACGGAGTTTCGTCGTCTCTCCTCTGCTAGTACTTGTCGACC
    TTCTCTCATATGCTCTTGTCTCAAAAGCAAATCCTCCCAAGAAACTACTCAGATTGAACA
    GTTGGGGAATGGAGAAGGGTTCTCAGTTTTGGCATCAGAGATTCCATGGGAGGATGAT
    AACATATGGAGCACATTTGCTCTTTACATGTTCTCTCTGCATATTCCTCTCAGTTTTGGG
    GGTTTATCCATTGTTGCCAACATACTCCACCGGCAGGTTCTTGATCCTCAGACCCAAGT
    GCTATCACTTGTGGTTCTCCAAATGGTAGAACTTGCAGGGACAGTCTTGCTGCTGAGG
    ACCACAGCGAAGCCTCAGTGCAAATCAATCAACTTTCTAAAGGGTAATAACGAAACAA
    GGGAAGGAAGAAACTGTGTGGTTGGCTCAGCATTGGGTTTGGGATGTCTTGTGGGCTT
    TATCTTCGTCACGTCGCTTGTAGCTGATCAACTCTTTGGCCCTAAGGCTGTACATGAAT
    CAGAATTGGAGAAGATAATGGTGAGCGGGGAAGTGGCGAGAAGCGGATGTTTTGCTC
    TCTACTGCGTAGTAGCTCCCATCCTTGAGGAGATAGTGTACAGACGCTTTCTCCTGAC
    CTCCTTAGCGTCGAGAATGGAATGGTGGAAGGCACTAGTGATCAGCTCAGGAGTATTT
    GCTGCAGGTCACTTCTCAGGTGAGGATTTTGTGCAGCTGTTTGGGATAGGTTGCGGTC
    TCGGGTTATGTTACAGCTGGTCAGGGAACTTAGCCTCATCAGTGCTCGTCCACTCCTT
    GTACAATGCATTGACACTTCTCTTCTCTTAG
    The At2g20725 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 2):
    MILGRWVSFSCGNTPVTNCSERRRHTEFRRLSSASTCRPSLICSCLKSKSSQETTQIEQLG
    NGEGFSVLASEIPWEDDNIWSTFALYMFSLHIPLSFGGLSIVANILHRQVLDPQTQVLSLVVL
    QMVELAGTVLLLRTTAKPQCKSINFLKGNNETREGRNCVVGSALGLGCLVGFIFVTSLVAD
    QLFGPKAVHESELEKIMVSGEVARSGCFALYCVVAPILEEIVYRRFLLTSLASRMEWWKAL
    VISSGVFAAGHFSGEDFVQLFGIGCGLGLCYSWSGNLASSVLVHSLYNALTLLFS
    cDNA sequence of At3g26085 from Arabidopsis (SEQ ID NO: 3):
    ATGGGTTCCATCGCTCTGCAATCTTGGAGCAGAGGAGCTTCAACTTCTCTTCATCTCCT
    TTTTCGTCCAGTTGGCTCGAACCCTAAGCTATATGATGCTCGAAGAGTACAATTTGATG
    TAAGAGCCTCTTCAAGTCGTAAATCACTTAAGAAACTCAAAAGAGAGTCACAACAAGGT
    AAAGACATAGGCTTAAGAAATGTTACAGATGAAGAAGTTTCTTCTCCAAGATTTGAGGA
    AGCTCAAGTTGATTCTTCAACTTCAAAGGACTCCATTGATGTTTTTGTTGCTGCTCCTC
    GAGACAAAGTGCTTCAGGCTTGCACTGTAACTTCCGGTTTGATGGCTGCACTAGGTCT
    GATCATCAGAAAGGCGTCTCATGTTGCTTCGACTGAAGGATTACTGGTTCCAGACTGC
    TCTATAGATGTACCATTTGGGTTTGAAACTTGGCATCTCGGTTTAATTGCTGGAATCGT
    TGTGTTTATATCGTCAAGTAGGTTCTTGCTACTTAAATCTTGGCCAGATTTTGCTGATTC
    TAGTGAAGCAGCAAACCGACAGATTCTCACTTCCCTCGAACCTCTAGATTACCTTGTTG
    TTGCAATGTTACCGGGAATAAGTGAGGAGCTGCTGTTTAGAGGTGCATTAATGCCTTT
    GTTCGGAACTAATTGGAATGGTATTGTAGCGGTTGGCCTCATTTTCGGTTTACTTCATC
    TCGGGAGCGGAAGAAAGTATTCTTTTGCAGTTTGGGCTTCGATTGTCGGTATAGTCTA
    CGGTTATGCAGCTGTTTTGTCGTCGAGTCTTATCGTTCCAATGGCCTCGCACGCACTC
    AACAATTTGGTGGGAGGTCTGTTGTGGCGATATAGTTCCAAGATCAAGTCATTGGAG-
    TAA
    The At3g26085 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 4):
    MGSIALQSWSRGASTSLHLLFRPVGSNPKLYDARRVQFDVRASSSRKSLKKLKRESQQGK
    DIGLRNVTDEEVSSPRFEEAQVDSSTSKDSIDVFVAAPRDKVLQACTVTSGLMAALGLIIRK
    ASHVASTEGLLVPDCSIDVPFGFETWHLGLIAGIVVFISSSRFLLLKSWPDFADSSEAANRQI
    LTSLEPLDYLVVAMLPGISEELLFRGALMPLFGTNWNGIVAVGLIFGLLHLGSGRKYSFAVW
    ASIVGIVYGYAAVLSSSLIVPMASHALNNLVGGLLWRYSSKIKSLE
    cDNA sequence of AtFACE-2 from Arabidopsis (SEQ ID NO: 5):
    ATGGCCACCGATGGCGAGAGTATCTCGATGTCGTTGGCGGTGGCTACCTGCGTCGCG
    ATGGCACTATTCTACGTTTTGATCCTTTACGTTCCCACCGTGATACTCCGTCTCCCGTC
    GGCTTCTTCTTACACCGAATTCATGATTCGGCGATTCATCTGCGCGGCCATTTGTACTG
    TAGCATCTCTCGTCTTCACAGCTTTTATACTTCCGATAAAAAGCTGGGAGGCCTCTTAT
    ATACTTGGAGTTTATGGCATAAGGAAAGATCATCTGTGGCAAGGAGTGGTGTATCCTCT
    TCTATTGACCTCGCTCGTTTATGCTGGGTCTTTGGTGTTGAAGTTGTTTACTCTCCTGG
    AATCATGGAAGGAAAATGGCGGAGGATGTAGTTCCTTTAATTACATCAGAAGCTTTTTC
    CAAACAATCCCTGCTTCGGTATTGACAAGTGCTTCTAATGTTTCCGTTTGGCGCAATTT
    TATCGTGGCACCAGTAACTGAGGAGCTGGTTTTCCGATCATGTATGATACCTTTGCTTC
    TGTGTGCTGGGTTTAGGATTAACACTGCCATCTTTCTGTGCCCAGTTCTCTTTAGCTTG
    GCTCACTTAAACCATTTTAGAGAGATGTACATCAGGCATAACCGCAGCTATCTCCGGG
    CTTCACTTATTGTTGGTCTTCAGCTTGGCTACACAGTCATTTTTGGTGCATATGCATCGT
    TTCTCTTCATCAGAACCGGACATCTTGCTGCTCCTTTGTTTGCTCATATATTTTGCAACT
    ACATGGGATTGCCTGTGCTATACGCAAATGGAAAAGGTTTGGTGAGTGCAGCGTTCTT
    AGGCGGTGTGGTTGGGTTCGTCTTACTTCTCTTTCCTTTAACAAAGCCTCTCATGTACA
    ACGATAGTACCAACGATTGTCCGTGTTGGCTTGGCTATTGTTTGTGGAATTGA
    The AtFACE-2 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 6):
    MATDGESISMSLAVATCVAMALFYVLILYVPTVILRLPSASSYTEFMIRRFICAAICTVASLVF
    TAFILPIKSWEASYILGVYGIRKDHLWQGVVYPLLLTSLVYAGSLVLKLFTLLESWKENGGG
    CSSFNYIRSFFQTIPASVLTSASNVSVWRNFIVAPVTEELVFRSCMIPLLLCAGFRINTAIFLC
    PVLFSLAHLNHFREMYIRHNRSYLRASLIVGLQLGYTVIFGAYASFLFIRTGHLAAPLFAHIFC
    NYMGLPVLYANGKGLVSAAFLGGVVGFVLLLFPLTKPLMYNDSTNDCPCWLGYCLWN
    cDNA sequence of ZM57353913 from corn (SEQ ID NO: 7):
    CGAAGCCACGCGACCGACTGTGTTACGATCCCAAATCTTCACTCCCGACGAAATCTAG
    AATCCAATGAGCAATCTCGACTGACGCCTGCTTCACCAGATTATGGCGACGCCGGCG
    GGCCTCCTTCTCGCCTCGCCGCCGGTGATATCAGGTGTCGCGGCGATGGCGGCGTG
    CGCCGCAATGGCAGTATTCTACGTCGCTGTCCTCTATGC
    CCCGACGGTCATCCTCCGGTTCCCACCCCCAACCTCACTCCGCACCTTCCTCCACCGT
    CGCTTCGCCTGTGCCGCCGTCGCATCCGCCGCCTCCGTCCTTGCCACTGCGTCCCTC
    CTCCGAGTCTGGAGCCTCAGCGACTTCGCTGATATGTTTGCTGTGTTCGGCATTAGGA
    AGGATCACTTGATTCAGGCCGTGGCTATTCCACTTCTCCTGACATCCCTAGTGTATGCT
    GGGTCATTCGTCGCTAGAGTGTGGCTCCTAGTGAGCTCGTGGGGCGGTGGCGATGAG
    GTGGAGATAGGCTGCGCACAGAGGCTTGCACAATGGATCCAAGCTGCTGTTGCGGAT
    GTTATGGTTTGGCGGAACTATGTAGTGGCACCATTTACTGAGGAGCTGGTTTTCAGGG
    CATGCATGATACCTCTTCTGCTCTGTGGGGGATTCAAAATGTCTACAATTATATTTCTGA
    GTCCAATCTTCTTCAGTCTAGCGCACTTGAACCATTTTTTCGAACTACACCAGCAGGGA
    TGTAACTTTATGAGAGCGCTATTGATTGTAGGTGTCCAGTTAGGCTACACTGTCATTTT
    TGGGTGGTATGCAACATTCTTGTTAATCCGAACAGGGAATCTGTTATGTCCAATTATTG
    CTCACGTCTTCTGTAATATGATGGGTTTACCTGTTTTCTCGTCACCACGAACAAAAGGA
    GCGGCATTGGTAGCGTTTCTGGCTGGTTCAATAGCCTTCTTTTGGCTGCTTTTCCCTGC
    AACAAGTCCTGAACTGTACAACAGCAGTTTTGATCGCTGCAGTTGCTGGCATGGCTTTT
    GCAATTGGAAATAACATAGAACTAGATTGGAAAGCAATGGGTCCTAACTTGAAGCTACT
    AACAATTGAAACCTCCAGGCCCTAGCTGACACTTCTGACGGATTTTCTATTTGCAGAAA
    CTCCATATGAATGTCTTAAAACGTTTTGTAGAAATGTGTCCCTTGTTTTAGCTTAAGACT
    CAAGAGCTTAAACTAGCAAAAGATTGTATTTTGCCACTTGTTAAATACGTGCTGATCAT
    GAAATCGCTGTCAATCCCTTCTCAAAGTGGAATTTGACTTTGTTGAGCTGCTTTTATTTA
    TATTGTGCTTGCTACTGCAGCGCCTAGAGTTTGTAGATTACACATCATGGACCCGTCTG
    ATATTGTAAACGAGAGACATGTTTCTAAGTTAATATGCTCCCTCCATTTATTTAAAAAAA
    AAAAAAA
    The ZM57353913 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 8):
    MATPAGLLLASPPVISGVAAMAACAAMAVFYVAVLYAPTVILRFPPPTSLRTFLHRRFACAA
    VASAASVLATASLLRVWSLSDFADMFAVFGIRKDHLIQAVAIPLLLTSLVYAGSFVARVWLL
    VSSWGGGDEVEIGCAQRLAQWIQAAVADVMVWRNYVVAPFTEELVFRACMIPLLLCGGF
    KMSTIIFLSPIFFSLAHLNHFFELHQGCNFMRALLIVGVQLGYTVIFGWYATFLLIRTGNLLC
    PIIAHVFCNMMGLPVFSSPRTKGAALVAFLAGSIAFFWLLFPATSPELYNSSFDRCSCWHG
    FCNWK
    cDNA sequence of ZM59252659 from corn (SEQ ID NO: 9):
    CCCAAATCTTCATTTCCGACGAAATCGAGAATCCAATGTGCAATCTCGACTGACGCCTG
    CTTCAACAGATTATGGCGACGCGGTGGGTCTCCTTCTCGCCTCGCCGCCGGAATATCA
    GGGTCGCGCGATGGGTCGTGCGCCAACGGAAGGATTCTACGTCGCTGTCCTCTATGC
    CCCGACGGTCATCCTCCGGGTCCCACCCCCAAGCTCACTCCGCACCTTCCTCCACCG
    TCGCTTCGCCTGTGCCGCCGTCGCATCCGCCGCCTCCGTCCTTGCCACTGCGTCCCT
    CCTCCGAATCTGGAGCCTCAGCGACTTCGCTGATATGTTTGCTGTGTTCGGCATTAGG
    AAGGATCACTTGATTCAGGCCGTGGCTATTCCACTTCTCCTGACATCCCTAGTGTATGC
    TGGGTCATTCGTCGCTAGAGTGTGGCTCCTAGTGAGCTCGTGGGGCGGTGGCGATGA
    GGTGGAGATAGGCTGCGCACAGAGGCTTGCACAATGGATCCAAGCTGCTGTTGCGGA
    TGTTATGGTTTGGCGGAACTATGTAGTGGCACCATTTACTGAGGAGCTGGTTTTCAGG
    GCATGCATGATACCTCTTCTGCTCTGTGGGGGATTCAAAATGTCTACAATTATATTTCT
    GAGTCCAATCTTCTTCAGTCTAGGTGTCCAGTTAGGCTACACTGTCATTTTTGGGTGGT
    ATGCAACATTCTTGTTAATCCGAACAGGGAATCTGTTATGTCCAATTACTGCTCACGTC
    TTCTGTAATATGATGGGTTTACCTGTTTTCTCGTCACCACGAACAAAAGGAGCGGCATT
    GGTAGCGTTTCTGGCTGGTTCAATAGCCTTCTTTTGGCTGCTTTTCCCTGCAACAAGTC
    CTGAACTGTACAACAGCAGTTTTGATCGCTGCAGTTGCTGGCATGGCTTTTGCAATTG
    GAAATAACATAGAACTAGATTGGAAAGCAATGGGTCCTAACTTGAAGCTACTAACAATT
    GAAACCTCCAGGCCCTAGCTGACACTGCTGACGGATTTTCTATTTGCAGAAACTCCATA
    TGAATGTCTTAAAACGTTTTGTAGAAATGTGTCCCTTGTTTTAGCTTAAGACTCGAGCTT
    AAACTAGCAAAAGATTGTATTTTGCCACTTGTTAAATACGTGCTGATCATGAAATCGCT
    GTCAATCCCTTCTCAAAGTGGAATTTGACTTTGTTGTAAAAAAAAAAA
    The ZM59252659 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 10):
    MGRAPTEGFYVAVLYAPTVILRVPPPSSLRTFLHRRFACAAVASAASVLATASLLRIWSLSD
    FADMFAVFGIRKDHLIQAVAIPLLLTSLVYAGSFVARVWLLVSSWGGGDEVEIGCAQRLAQ
    WIQAAVADVMVWRNYVVAPFTEELVFRACMIPLLLCGGFKMSTIIFLSPIFFSLGVQLGYTVI
    FGWYATFLLIRTGNLLCPITAHVFCNMMGLPVFSSPRTKGAALVAFLAGSIAFFWLLFPATS
    PELYNSSFDRCSCWHGFCNWK
    cDNA sequence of CASAR82A from pepper (SEQ ID NO: 11):
    ATGGTGTCTAAGTCCTCAATCTTCATTTGCCTGAGCCTTATCATCCTCGTGATCATGTC
    TACCCAGATCGTGGCTAGAGAGATGACCAGTGAAGCTTCTGCTTCACTCACACAGGCA
    ATGAACGGGAACAATATCTCTGAGACCAAGAAAGTGGGTCGTCACTTGGTGAAGGGCT
    TGGATAAGATCTTCAAGGCTGGAAAGGTGATCTACTGCAAGACCTGCAAAACCTGCCA
    CGGTCGCTGCGATTACTGTTGCGCC
    The CASAR82A cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 12):
    MVSKSSIFICLSLIILVIMSTQIVAREMTSEASASLTQAMNGNNISETKKVGRHLVKGLDKIFK
    AGKVIYCKTCKTCHGRCDYCCA
    cDNA sequence of b3358 from E.coli (SEQ ID NO: 13):
    ATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGG
    TGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCT
    CTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATA
    CGCCTCATAAACGTTTTTTCAAACGCTTAATCATTGGTGCGTCGCTGTTTGCCACCTGT
    AGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCG
    GATTAACGCTGGTACTTGGCGTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCT
    TCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTC
    TGGGAACCGTTGCTCATCTATGCGTTGGGCACTCTCTGGTACGGATTGTTTAACTGGT
    TTTGGTTCTGGATCTGGCGCGAACAACCGCTGCGCGAGTCACTAAGTCTGCTGTACCG
    TGAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCACACCGACCCT
    GAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGGTCGATCTAATTACC
    CAGTGCTATCAGCAAATGCATATGCTTTCCGCGCAAAATAATACTGACTACAAGCGGAT
    GCTGCGTATTTTCCAGGAGGCGCTGGATTTACAGGAACATATTTCGGTCAGTTTGCAT
    CAGCCGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGC
    TGGAATGCGCAAACCGTCGCCGCTCGCCTGCGCGTGCTGGCTGATGACATTCTTTAC
    CATCGCCTGCCAACGCGTTTTACGATGGAAAAGCAAATTGGCGCACTGGAAAAAATCG
    CCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCAT
    CGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGACTTACTGGCCGATAA
    ACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCG
    GCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCAGCCTGATGGGC
    ACCGCGCTGCATCTGCCGAAGTCGTACTGGATCCTGATGACGGTATTGCTGGTGACAC
    AAAATGGCTATGGCGCAACCCGTCTGAGGATTGTGAATCGCTCCGTGGGAACCGTGG
    TCGGGTTAATCATTGCGGGCGTGGCGCTGCACTTTAAAATTCCCGAAGGTTACACCCT
    GACGTTGATGCTGATTACCACCCTCGCCAGCTACCTGATATTGCGCAAAAACTACGGC
    TGGGCGACGGTCGGTTTTACTATTACCGCAGTGTATACCCTGCAACTATTGTGGTTGA
    ACGGCGAGCAATACATCCTTCCGCGTCTTATCGATACCATTATTGGTTGTTTAATTGCT
    TTCGGCGGTACTGTCTGGCTGTGGCCGCAGTGGCAGAGCGGGTTATTGCGTAAAAAC
    GCCCATGATGCTTTAGAAGCCTATCAGGAAGCGATTCGCTTGATTCTTAGCGAGGATC
    CGCAACCTACGCCACTGGCCTGGCAGCGAATGCGGGTAAATCAGGCACATAACACTC
    TGTATAACTCATTGAATCAGGCGATGCAGGAACCGGCGTTTAACAGCCATTATCTGGC
    AGATATGAAACTGTGGGTAACGCACAGCCAGTTTATTGTTGAGCATATTAATGCCATGA
    CCACGCTGGCGCGGGAACACCGGGCATTGCCACCTGAACTGGCACAAGAGTATTTAC
    AGTCTTGTGAAATCGCCATTCAGCGTTGTCAGCAGCGACTGGAGTATGACGAACCGGG
    TAGTTCTGGCGATGCCAATATCATGGATGCGCCGGAGATGCAGCCGCACGAAGGCGC
    GGCAGGTACGCTGGAGCAGCATTTACAGCGGGTTATTGGTCATCTGAACACCATGCAC
    ACCATTTCGTCGATGGCATGGCGTCAGCGACCGCATCACGGGATTTGGCTGAGTCGC
    AAGTTGCGGGATTCGAAGGCGTAA
    The b3358 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 14):
    MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVPACCNIAGLDTPHKRFFKR
    LIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTAELGPLHAKLLPASLLAAIFTLSLAG
    YMPVWEPLLIYALGTLWYGLFNWFWFWIWREQPLRESLSLLYRELADYCEAKYSLLTQHT
    DPEKALPPLLVRQQKAVDLITQCYQQMHMLSAQNNTDYKRMLRIFQEALDLQEHISVSLHQ
    PEEVQKLVERSHAEEVIRWNAQTVAARLRVLADDILYHRLPTRFTMEKQIGALEKIARQHP
    DNPVGQFCYWHFSRIARVLRTQKPLYARDLLADKQRRMPLLPALKSYLSLKSPALRNAGR
    LSVMLSVASLMGTALHLPKSYWILMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHF
    KIPEGYILTLMLITTLASYLILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIA
    FGGTVWLWPQWQSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLY
    NSLNQAMQEPAFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEI
    AIQRCQQRLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMA
    WRQRPHHGIWLSRKLRDSKA
    cDNA sequence of EST564 from moss (SEQ ID NO: 15):
    ATGTCATGCGACGTTCTCTGCCAATCTTTCAAGGAGGTAGAGGGCAAGTTCTTGGAAA
    TCGTCGAAAGGGCTTGGGCCGTCAAGCCGCAAATTGCCGCTGTTGGATCTTGTTGTTT
    GGTGGGAGCCGTATGGGATTCCAAACTGTACATCGCTAGTCTTGGAGATTCTCGAGCT
    GTTTTAGGTAGTTGCTCTCGTGACACTGGCCTTCCAGTTGCTAAGCAAATTTCAACAGA
    GCACAACGCAAGCATCGAGTCTATCCGGAATGAGTTGTTCGCAAAGCATAGTGATGAT
    CCGCAGATCGTGGTTTTGAAGCATGGAGTGTGGCGTGTGAAGGGTATTATTCAGATTT
    CACGCTCAATTGGTGATTTTTACTTGAAGAAAGCCGAATTTAATCAGCCGCCTCTTATA
    GCCAGGTTCCGG
    CTTCCAGATCCCCTCAAGAGACCTGTCATAAGCTCAGAGCCGGAGTGCAACGTCATTA
    CACTCGGCCCGGATGACGAATTCGTCATTTTTGCATCTGATGGCCTTTGGGAGCACTT
    GAGCAGCAAAGAGGCCGTAGACATTGTGTATAGTCATCCCCGGGCTGGGATTGCCAG
    GCGTCTGATCAAAGCTGCTCTTCAAAAAGCTGCTACTAAACGTGAAATGCGGTACTCT
    GATTTGAAAGGGATTGAGCGCGGGATACGACGGCATTTTCATGATGACATAACTGTTG
    TGGTTCTTTATTTGGACACTAAACTGCTCAACAGAGGTGGTAGTATTTCTAATCATATTT
    CTTCGAAATGTCCAATTGACATGCCAAAAGGCGATAACCCTCCGTCGTTAGTTAGCTCT
    AACATGAACTTAGCTTTTAACAAATAA
    The EST564 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 16):
    MSCDVLCQSFKEVEGKFLEIVERAWAVKPQIAAVGSCCLVGAVWDSKLYIASLGDSRAVL
    GSCSRDTGLPVAKQISTEHNASIESIRNELFAKHSDDPQIVVLKHGVWRVKGIIQISRSIGDF
    YLKKAEFNQPPLIARFRLPDPLKRPVISSEPECNVITLGPDDEFVIFASDGLWEHLSSKEAV
    DIVYSHPRAGIARRLIKAALQKAATKREMRYSDLKGIERGIRRHFHDDITVVVLYLDTKLLNR
    GGSISNHISSKCPIDMPKGDNPPSLVSSNMNLAFNK
    cDNA sequence of BN49502266 from canola (SEQ ID NO: 17):
    CCAATAATCAAATCAAAACCCTTTCGATCAGTTGTTNCAGGAAAAAAAAAAACCCTTTC
    GATCTCATTCCATTTCGAATCAGAAAACCCTAGCAATTGACGATGTTGCGAGCTTTAGC
    GCGGCCTCTCGAACGGTGTTTAGGAAGCAGAGCGAGCGGCGACGGTTTGCTCTGGCA
    ATCGGAGTTGAAACCACACGCCGGCGGAGATTACTCGATCGCGGTGGTTCAAGCCAA
    TTCTAGCCTAGAGGATCAGAGTCAGGTGTTCACGTCTTCCTCCGCTACTTACGTCGGC
    GTCTACGACGGCCATGGCGGACCCGAAGCTTCTAGATTCGTTAACAGACATCTCTTTC
    CTTATATTCAGAAGTTCGCAAAAGAACATGGAGGACTGTCTGCAGACGTTATTAAAAAA
    GCATTCAAAGAAACTGAAGAGGATTTTTGCGGTATGGTTAAACGCTCACTTCCCATGAA
    GCCACAGATGGCTACTGTAGGATCTTGCTGTCTCTTTGGTGCCATCTCTAACGGCACG
    CTCTATGTCGCGAATCTTGGAGACTCGAGAGCCGTTCTTGGGAGCGTTGTTGCAGGG
    GATGATAGTAATAGTAGTAACAAGGGTGCTGCAGCTGAACGGTTGTCCACTGATCATA
    ACGTTGCTGTTGAAGAAGTGAGGAAGGAGGTTAAGGAACTTAACCCGGATGATTCGCA
    GATCGTCATGTACATTCGTGGAGTTTGGAGGATTAAAGGCATTATTCAGGTATCTAGAT
    CAATTGGGGATGTTTACTTGAAGAAACCGGAGTTTTACAGGGATCCGATATTCCAGCAA
    CATGGAAATCACATTCCTTTGAGGAGACCCGCGATGACAGCTGAACCGTCCATTATAG
    TAAGGAAGCTTAAGCCGCAAGACTTGTTTCTGATATTTGCATCAGATGGTCTCTGGGAG
    CATCTTAGTGATGAAGCAGCAGTAGAAATTGTACTCAAACACCCAAGAACTGGGATTG
    CAAGAAAACTTGTAAGAGCAGCTCTTGAAGAAGCAGCAAGGAAGAGAGAAATGAGATA
    TGGAGATATAAAGAAAATAGCCAAAGGGGTTAGAAGACATTTCCATGACGACATAAGC
    GTCGTTGTAGTTTATCTTGATCAACAAAAAACCACTTCTTCATCGAATGATAGATTGATC
    CAGAAAGGAGGAATCACTGCTCCACCGGATATCTACTCGTTACGTTCAGATGAAGCTG
    AGCAACGACGGCTACTCAATGTGTTATATTGATACTCTCTGGTTAGAGGGATACAACTT
    GTTTACATATTTGTTTAATCTTTTACAAAGAATGTTTGTTCTTTTTTCTTTCTTTTTTTAAT
    ATTTGGAGTTGGATTTGTATATTCTTTTTACCAGCAAGGAACGAAAACCCTTCTCTTTTG
    GGGGCAAAACAGTTTTGGTTTTGACAAACAATATAAAGTGAAACCGTTTGCAAAAAAAA
    AAAAAAAAA
    The BN49502266 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 18):
    MLRALARPLERCLGSRASGDGLLWQSELKPHAGGDYSIAVVQANSSLEDQSQVFTSSSAT
    YVGVYDGHGGPEASRFVNRHLFPYIQKFAKEHGGLSADVIKKAFKETEEDFCGMVKRSLP
    MKPQMATVGSCCLFGAISNGTLYVANLGDSRAVLGSVVAGDDSNSSNKGAAAERLSTDH
    NVAVEEVRKEVKELNPDDSQIVMYIRGVWRIKGIIQVSRSIGDVYLKKPEFYRDPIFQQHGN
    HIPLRRPAMTAEPSIIVRKLKPQDLFLIFASDGLWEHLSDEAAVEIVLKHPRTGIARKLVRAAL
    EEAARKREMRYGDIKKIAKGVRRHFHDDISVVVVYLDQQKTTSSSNDRLIQKGGITAPPDIY
    SLRSDEAEQRRLLNVLY
    cDNA sequence of GM49788080 from soybean (SEQ ID NO: 19):
    TCCCGGGTCGACGATTTCGTGGTTACGGGGCGGAAGGAAGGGCTGCTGTGGTACAAG
    GATGCGGGGCAGCACTTGTTTGGTGAATACTCAATGGCTGTTGTCCAGGCCAACAACC
    TGCTCGAGGACCAGAGCCAGATTGAGTCTGGTCCTCTCAGCCTGCTTGACACTGGCC
    CTTATGGGACCTTTGTTGGTGTATATGATGGACACGGTGGGCCCGAGACGTCGCGCTA
    CGTCTGTGATCATCTCTTCCAACATCTAAAACGATTTGCATCTGAGCAGAAGTCCATGT
    CTATGGAGGTTATTCGGAAGGCATACCAAGCCACAGAAGAAGGTTTTTTGTCAGTGGT
    TACCAAACAGTGGCCCATGAATCCCCAAATTGCTGCTGTGGGATCTTGTTGTTTGGTTG
    GTGTGATTTGTGGTGGTATCCTCTATATTGCTAACCTTGGTGATTCCCGTGCTGTGCTT
    GGCCGGGTGGTCAGAGCAACTGGGGAGGTTTTGGCGATCCAGCTTTCGTCAGAGCAT
    AATGTGGCCATAGAATCTGTGAGACAAGAGATGCATTCTTTGCATCCGGATGACTCAAA
    AATTGTGGTTCTAAAGCACAATGTATGGCGGGTGAAGGGTCTGATACAGATTTCTAGAT
    CCATTGGCGATGTATACCTAAAAAAGGCTGAATTTAACAAGGAACCGTTGTATGCTAAG
    TTTCGTGTGCGGGAAGGTTTTAAGAGGCCCATTTTGAGCTCTGACCCATCAATTTCTGT
    CCATGAACTTCAACAGCATGATCAATTTCTCATATTTGCTTCTGATGGTCTTTGGGAACA
    CCTTAGCAATCAGGATGCCGTTGATATAGTTCAAAACAACCCACACAATGGAATTGCTC
    GGAGGCTCATCAAAGCTGCGTTGCAAGAAGCAGCAAAAAAGAGAGAGATGAGGTACT
    CTGATTTGAAGAAAATTGACCGTGGTGTCCGCCGGCATTTCCATGATGACATCACAGTT
    GTAGTTGTATTTCTTGACTCCAATCTTGTCAGCAGAGCCAGCTCAGTAAGAGGTCCTCC
    TTTATCGGTGAGAGGAGGTGGTGTTCCCCTACCTTCTAGAACTTTGGCTCCCTGTGCT
    GCACCTATGGAAACTTAGTTCAGGTTGATGAAGCTGGCTGTATGATCTGTTATGCTTCT
    ATTTAGTGTTGTACCCTTAGCAGACATTGAGCTCTGGTGATCCACCAGATTGTATATCC
    AATTTAACAGAGATTGAAAAAATGTTCGTTCA
    ATTAGTACAATGTTACAAGTGACTTGGGGTATGTAGCTTGCGTGAGTAAAGCATCATG-
    GAA
    The GM49788080 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 20):
    MAVVQANNLLEDQSQIESGPLSLLDTGPYGTFVGVYDGHGGPETSRYVCDHLFQHLKRFA
    SEQKSMSMEVIRKAYQATEEGFLSVVTKQWPMNPQIAAVGSCCLVGVICGGILYIANLGDS
    RAVLGRVVRATGEVLAIQLSSEHNVAIESVRQEMHSLHPDDSKIVVLKHNVWRVKGLIQISR
    SIGDVYLKKAEFNKEPLYAKFRVREGFKRPILSSDPSISVHELQQHDQFLIFASDGLWEHLS
    NQDAVDIVQNNPHNGIARRLIKAALQEAAKKREMRYSDLKKIDRGVRRHFHDDITVVVVFL
    DSNLVSRASSVRGPPLSVRGGGVPLPSRTLAPCAAPMET
    cDNA sequence of GM53049821 from soybean (SEQ ID NO: 21):
    TGCTCCTCTACCACCGAACACANCCCCGGCCACCACCGAACGCTAACGTGCGCCCCT
    TCCTTACCCTGCGCCTCGGCACTCTCCCTTATTCCCCCTCCTTCATAAGCTCCGCGTTA
    ACCGTCCTCTCTCTCTCTCTCTCTCGGATCGGAGCGAGACTGGCGGCTCCGGCGTTG
    GGGGCGTTAGGGTTAGGGTTAGGGTTTCCAAGAGATG
    TGGTATGCTCCAGGCATTGATGAATCTGTTCTCGCTGTGTTGGAAGCCATTTGGCCGC
    GATGCTGCTGATCGAATCGATTCCATCGGAGTTACCGGAAGAGAAGGCAAAGACGGC
    TTGCTTTGGTTCCGCGACGGCGGAAAATATGGCTCTGGCGATTTCTCCATGGCCGTCG
    TTCAGGCCAACCAGGTTCTCGAAGACCAGAGCCAGATCGAGTCTGGTCCTCTCGGCA
    CCTTCGTCGGCATCTACGACGGTCACGGAGGACCCGACGCCTCAAGATACGTTTGCG
    ATCACTTGTTTCGCCATTTTCAAGCAATATCAGCTGAGTCACGCGGGGTTGTGACAACT
    GAGACAATCGAAAGAGCATTTCGCCAAACAGAAGAGGGGTACATGGCCCTCGTGTCA
    GGCTCGTGGAATGCTCGACCTCATATTGCAAGTGCTGGGACCTGTTGTCTAGTTGGAG
    TGATATTTCAGCAGACACTCTTTGTGGCAAACGCTGGAGATTCCCGTGTTGTATTGGGT
    AAGAAAGTTGGCAACACTGGAGGTATGGCTGCAATTCAGCTGTCTACAGAACACAATG
    CAAATCTTGAGGCTGTTAGGCAGGAACTTAAAGAATTACATCCTCATGATCCCCAAATT
    GTTGTCCTCAAACATGGAGTGTGGAGAGTAAAAGGCATTATTCAGGTTTCTAGATCTAT
    AGGTGATGTATATTTGAAGCATGCACAGTTTAACCGAGAACCACTTAATGCAAAATTCA
    GACTTCCTGAACCGATGAACATGCCTATCTTGAGTGCTAATCCCACTATTCTTTCTCAT
    GCTCTCCAACCAAATGATTCCTTCCTTATATTTGCATCTGATGGTTTATGGGAGCATTTG
    AGTAACGAGAAAGCTGTGGATATTGTAAACAGCAATCCACATGCGGGTAGTGCCAAGA
    GACTTATCAAGGCTGCTCTCCATGAAGCAGCAAGAAAACGAGAAATGCGATATTCAGA
    CCTCCGTAAGATTGACAAGAAAGTTCGACGCCATTTTCATGATGATATATCCGTTATTG
    TTTTATTCTTAAATCACGACCTTATTTCCAGAGGCACAGTGCTAGACCCGACACTTTCA
    ATTCGAAGCGCTCTCGATCACTGACTTGTATCACTGTAAGCAGTCTTGTACGAGTTTTT
    GGCAACTGTACCGATACCTGAAGCATTGGTAGGTACCTGGCTATAATATGTCATTTCTA
    TGGCACATATGGCTTCTGGTACCGACATCATTCT
    TGAGGCACGAGAATTTATTAAGTTATAACATATTATTAGAAATTTATTCATAAAGAGGAA
    AAAAATAAATACAAAAATATCTTATTCCCTTTTCTAACCTTATAGTTTTACCCGAAATACT
    GGATTTTATTTATTTGTTTGTTTTTTTGGCTGAACATAGCTAATCGAACAGCATGTTGAT
    TGAATTCAAAGTTATTTTACAACAAATTATATGGAAAAAAAAAAAAAAA
    The GM53049821 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 22):
    MLQALMNLFSLCWKPFGRDAADRIDSIGVTGREGKDGLLWFRDGGKYGSGDFSMAVVQA
    NQVLEDQSQIESGPLGTFVGIYDGHGGPDASRYVCDHLFRHFQAISAESRGVVTTETIERA
    FRQTEEGYMALVSGSWNARPHIASAGTCCLVGVIFQQTLFVANAGDSRVVLGKKVGNTG
    GMAAIQLSTEHNANLEAVRQELKELHPHDPQIVVLKHGVWRVKGIIQVSRSIGDVYLKHAQ
    FNREPLNAKFRLPEPMNMPILSANPTILSHALQPNDSFLIFASDGLWEHLSNEKAVDIVNSN
    PHAGSAKRLIKAALHEAARKREMRYSDLRKIDKKVRRHFHDDISVIVLFLNHDLISRGTVLDP
    TLSIRSALDH
    cDNA sequence of ZM58462719 from corn (SEQ ID NO: 23):
    CGTGGCGACGCCCAAATCGAGCGACCTGATCGAGGCCCCTCGCCCCTACTCGCTGAA
    TCCCAATCCGAGCCCGCCAATTGGGCGCCCCCCCCCGCCCACGCAAAGGACAGATAG
    AAGAAAATTATTGGCGCTCTGACAAATCCAACTGAGGTTTTCTTGGACTACAGATGAAG
    CGGGCTCGAAGGGCGTATGTGCAAGAGATGACTGATGAGGGATGCTAGTGAAATTGA
    TGAACTTGTTACGGGCGTGCTGGCGACCGTCATCGAACCGGCATGCCCGAACAGGCT
    CAGATGTTACCGGTAGGCAGGATGGACTTCTATGGTACAAGGACGCCGGGCAACATG
    TCAATGGGGAGTTCTCCATGGCTGTTGTTCAGGCAAATAACTTACTTGAGGACCAGTG
    TCAGATCGAGTCGGGCCCACTGAGTTTTCTAGATTCTGGACCATATGGCACTTTCGTT
    GGTGTTTACGATGGGCATGGTGGTCCAGAGACGGCCTGCTATATCAATGATCATCTTT
    TCCAGAATCTGAAAAGATTTGCATCTGAACAGAATGCAATGTCTGCTGATGTACTGAAG
    AAGGCATATGAAGCTACAGAAGATGGATTCTTCTCCATTGTTACCAAACAATGGCCTGT
    AAAGCCTCAGATAGCAGCTGTCGGCTCATGCTGCCTGGTCGGTGTAATTTGTGGTGGC
    ATGCTTTATGTTGCCAATGTTGGGGATTCCCGTGTCGTTTTAGGAAAACATGTTAAGGC
    CACTGGAGAAGTTTTGGCTGTCCAACTGTCAGCAGAACATAATGTTAGTATTGCGTCC
    GTGAGAAAAGAACTGCAGTCAATGCACCCAGAAGATAGGCACATTGTTGTTCTCAAGC
    ACAATGTTTGGCGTGTTAAAGGACTAATTCAGGTTTGTAGATCAATTGGTGATGCATAT
    CTCAAAAAGCAAGAGTTCAACAGGGAACCCCTATATGCAAAATTTCGCCTCCGTGAAC
    CTTTTCACAAGCCAATACTAAGTTCAGAACCATCAATCAGTGTGCAACCACTACAACCA
    CACGACCAGTTTCTCATATTTGCATCTGATGGACTTTGGGAGCAGTTAACCAACCAAGA
    GGCAGTTGATATTGTTCGAAGTAGCCCCCGCAGTGGCTGTGCTAGGAGGCTGATAAG
    AGCGGCACTGCAAGAGGCAGCCAAGAAAAGAGAGATGAGGTACTCGGACCTCAAGAA
    GATTGACCGCGGTGTTCGCCGCCACTTCCACGACGACATAACAGTCATAGTAGTGTTC
    CTTGACTCCGGCCTCGTAAGCCAGGCGAGCACACACCGAGGTCCAACTCTTTCCTTGC
    GAGGCGGTGGCGGCAGCGCTGGCCTGCGCAGCAACACACTTGCACCTACGTGACTAT
    AAAGTGCCTGGTGGAGTGGAGGCTACTGACTGAAGGTGGTTTTCTTTCCTTGTGTCGA
    ATGTGTTATATATGTACTTGTACCAGCCAAGATCATTCATCCCCCCCCCTAAAATGGTG
    TAAAGAAGTAGGAGAGGCGCCGAAGTTCCTCACCAGCGTATCTGAATGCCCTCAATGG
    TGTCAAGTTGTGGACTCAAGTGGATAGCTTCGCTGAATCTTCTGATGATGCTCTGTGGA
    AAGCTCGAATCCTTTCCACCTGAAAAAGCAAGTAATATGTCTTCCAGTGCTGGAATTAA
    CCCCTAGTGCATATATATATGTATGAAATAATAATAAGGCAAAAGGAGGAGTAACTTAT
    TTAACTAATGCTGTGAGGTGTATTTATGTTTTGTATGTGTACTGCTTTTGACTGCTACTG
    CATCTACTGTTGTTAATTGACCACTGGTGAAGTGAAATCACTGGTTTCGTAAAAAAAAA
    AAAAAA
    The ZM58482719 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 24):
    MLVKLMNLLRACWRPSSNRHARTGSDVTGRQDGLLWYKDAGQHVNGEFSMAVVQANNL
    LEDQCQIESGPLSFLDSGPYGTFVGVYDGHGGPErACYINDHLFQNLKRFASEQNAMSAD
    VLKKAYEATEDGFFSIVTKQWPVKPQIAAVGSCCLVGVICGGMLYVANVGDSRVVLGKHV
    KATGEVLAVQLSAEHNVSIASVRKELQSMHPEDRHIVVLKHNVWRVKGLIQVCRSIGDAYL
    KKQEFNREPLYAKFRLREPFHKPILSSEPSISVQPLQPHDQFLIFASDGLWEQLTNQEAVDI
    VRSSPRSGCARRLIRAALQEAAKKREMRYSDLKKIDRGVRRHFHDDITVIVVFLDSGLVSQ
    ASTHRGPTLSLRGGGGSAGLRSNTLAPT
    cDNA sequence of ZM61092633 from corn (SEQ ID NO: 25):
    AGCTTCCTCCCTCTTCCCTGGTCTGGTCGCTTCTCCTGTAGCTGTAATTTTTGAGAGTC
    CCTCTCAAACTTTGCTTGCTTGCGCTCTCCATATATCCTGTGGATCGGAGAGGATGCTC
    TGATCTACCTGTCTGTTCTTCGATCGAGTCTGAGAGATTTGGGAGGAGGAGGGAAACA
    AAGCGAAAGAGCCCATCTTTTTTGTCTTTTTGGTTCGGTTTCGTGGTTGCTTCTTTTGG
    ACCCCGCGGAGGAGCCCACCGTTTCTACAAAAACCCAATCTTTGCTGCCTTCTCAGCG
    GTCGAGATCGATAGGTTTCCAGATCTGAGGCTCCGTGTTCTGGCTGTGAGATCGGAG
    GCGCAGCAATCCGAGCACGCAGCTAGTAGGGAAAGTATCCGAGAAAAGTTGCAGATT
    TTGCTGGGGGCAACGGAGCGAGAACAAGTTACTGCAGAAGGAAAGGGCAAAGGTGG
    GGGAGGCGCCGGAGATGAGGGATGCTATCAGCTCTGATGGATTATTTGAAATCTTGCT
    GGGGTCCGGCATCACCGGCTGGGCGTCCCCGCAAAGGATCGGATGCCACCGGCCGC
    CAGGACGGGCTCCTGTGGTACAAGGACGGCGGGCAGGTCGTCGATGGTGAGTTCTC
    CATGGCCGTGGTCCAGGCCAATAACCTATTGGAGGACCATAGCCAGGTTGAATCCGG
    GCCGCTTAGCACATCGGAGCCTGGACTGCAAGGCACCTTCGTCGGGGTCTACGATGG
    GCACGGTGGCCCGGAGACAGCGCGTTACATCAATGACCATCTCTTCAACCACTTGAG
    GAGATTCGCATCTGAGCACAAGTGCATGTCAGCGGATGTGATTCGGAAGGCATTCCGA
    GCGACTGAGGAGGGTTTCATTTCTGTGGTTAGTAACCAATGGTCATTGAGACCTCAATT
    AGCAGCTGTAGGCTCTTGCTGTCTAGTTGGTGTGGTTTGCAGCGGAACTCTATATGTT
    GCAAACCTTGGGGACTCCCGTGCTGTTCTGGGGAGACTTGTCAAGGGAACTGGGGAG
    GTTTTGGCAATGCAGCTCTCAGCAGAACACAATGCATCCTATGAGGAGGTTAGACGAG
    AGCTGCAGGCATCACATCCTGATGATCCCCATATTGTGGTCCTAAAACACAATGTTTGG
    CGTGTAAAGGGTATTATCCAGATAACAAGGTCAATTGGAGATGTGTATCTGAAGAAACC
    AGAATTTAATAGAGAACCTTTGCACAGCAAGTTTCGTCTTCAGGAAACTTTTAGGAGAC
    CACTTCTTAGTTCTGATCCAGCAATTACTGTCCACCAAATACAGCCAACTGATAAGTTC
    ATCATTTTTGCATCTGATGGACTCTGGGAACATCTTAGTAATCAGGAAGTGGTTGACAT
    GGTCCAAAGTAGCCCGCGTAATGGAATCGCACGAAAGTTAGTAAAGTCTGCAGTGCAG
    GAAGCAGCGAAGAAGAGGGAGATGCGGTATTCAGACCTCAAGAAAGTTGATCGGGGG
    GTGAGGCGGCACTTCCACGACGATATAACTGTCATTGTGGTATTTTTCGATTCAAACGC
    CATGACAACTGCTGCCTGGAGCAGACCCTCGGTCTCTCTCCGAGGGGGTGGGTTTCC
    AATCCATTCAAACACCCTTGCTCCATTCTCGGTTCCTACAGAGCTAAACAACTCCTACT
    GAAACCACGCGGTATGTGAAGGAGCCAGGCAAGAGGATAAAAAAAAAGTAAAGGAAA
    ACGGAGAAGGAAAAACAGCTGTTGTGATCAGTTGTAGTGTATTTCACCGTTCATGTTCA
    TTTAAAACATTTTTTAGATTCTCAAGTCTCAACCTGGTGACCAGTGCACTGATAGCAAG
    GTATAAGATTAGATTATTCTTAGCTTTTTTATCCTCTTTTTTTTTTCTCGTCCTTACCCTTT
    AGATTCACTCATGGGATATCCGATATCAGGTGCTTGTACATTCTTTGGTTCAACTTGTG
    ATAATAGTTCATCGCCCCCCTCTTTTCGCAAAAAAAAAAA
    The ZM61092633 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 26):
    MLSALMDYLKSCWGPASPAGRPRKGSDATGRQDGLLWYKDGGQVVDGEFSMAVVQAN
    NLLEDHSQVESGPLSTSEPGLQGTFVGVYDGHGGPETARYINDHLFNHLRRFASEHKCMS
    ADVIRKAFRATEEGFISVVSNQWSLRPQLAAVGSCCLVGVVCSGTLYVANLGDSRAVLGR
    LVKGTGEVLAMQLSAEHNASYEEVRRELQASHPDDPHIVVLKHNVWRVKGIIQITRSIGDV
    YLKKPEFNREPLHSKFRLQETFRRPLLSSDPAITVHQIQPTDKFIIFASDGLWEHLSNQEVV
    DMVQSSPRNGIARKLVKSAVQEAAKKREMRYSDLKKVDRGVRRHFHDDITVIVVFFDSNA
    MTTAAWSRPSVSLRGGGFPIHSNTLAPFSVPTELNNSY
    cDNA sequence of ZM62016485 from corn (SEQ ID NO: 27):
    TGTCTTGCTGCTGGCGCGCCGGGGCTCCGATTGCGCTCCAGATCTGAGGCACCTGCT
    CGGTGGATTCCAGGAATCCGAGCACCAACTCGACAGGGGAGTTCTCAGGGTAAAGAG
    GCTGAGAGCGCGTTGGAGATTTGGACTATAAGAGCGAGCGAGCGAGCTGGGTGCCTT
    GCTGCCTTGAGGACGCCGTCAAGAAACCGCGTGGAGGGGAGGGCGATGAGATGAGG
    GATGCTGGCCGCGGTGATGGACTACTTCAGCACCTGCTGGGGCCCGCGGTCTCGTGC
    GGGGCACCGGGGCAAGGGCTCCGACGCCGCCGGCCGGCAGGACGGCCTCCTCTGG
    TACAAGGACGCCGGGCAGCTCGTCACCGGGGGGTTCTCCATGGCCGTGGTGCAGGC
    CAACCAGCTGCTTGAGGACCAGAGCCAGGTGGAGTCCGGATCGCTCTCCCTGGCTGA
    CTACGGCCCGCAGGGCACCTTCGTCGGCGTCTATGATGGCCATGGCGGCCCGGAGA
    CGTCCCGGTTCATCAATGACCACCTCTTCAACCATCTCAGGAGATTCGCAACTGAGCA
    CAAGTCCATGTCAGCAGACGTGATCCGGAAAGCTTTCCAAGAAACTGAGGAGGGCTTT
    CTTTCTCTAGTCATCAAGGAATGGTCTTTCAAGCCTCAGATTGCATCAGTTGGCTCCTG
    TTGCCTTGTTGGTGTAATCTGTGCTGGGACTCTCTATGTTGCAAACCTGGGCGACTCG
    CGTGCAGTTCTTGGAAGGCTTGTGAAAGCAACTGGAGAGGTTCTGGCCACTCAGTTGT
    CAGCGGAGCACAATGCATGCTATGAAGAAGTTAGACAAGAGCTGCAGTCATCACATCC
    TGATGATCCACGTATTGTGGTTCTCAAACATAACGTTTGGCGAGTGAAGGGTCTCATCC
    AGATCTCAAGATCTATCGGAGATGTATATCTAAAGAAACCGGAGTATAACAGAGAACCT
    CTTCACAGCAAGTTTCGGCTTCGAGAAACCTTCCAGAAGCCGATTCTTAGTTCTGAACC
    TCAAATTACTGAACACCGAATACAGCCAAACGATCAGTTTGTTATATTTGCTTCCGATG
    GTCTATGGGAGCACCTCAGCAATCAGGAAGCTGTTGACCTTGTCCAAAGTAGTCCCCG
    TAATGGAATCGCTCGGAGACTAGTGAAAGCCGCGATGCAAGAAGCTGCCAAGAAGAG
    GGAGATGAGATACTCAGACCTCAAGAAGATCGACCGTGGCGTGAGGAGGCATTTCCA
    CGACGATATAACCGTCGTCGTGGTGTTCCTCGACTCGGATGCCATGAGCAAAGCTAGC
    TGGAGCAAGAGCCCCTCGTTTTCTCTCCGAGGGGGCGGCGTCACCCTTCCCGCCAAG
    TCCCTCGCACCCTTCTCGGCTCCGGCACAGTTGAACGGCACCCACTGAAGCTGCTACT
    GCTCTTGAAAAGAAGGGCACAGTGCAGATCTGCTAGAGATGATGAGAGAAGCAGCAAT
    CAAGTGTAGCTGTTGCTCGTACACCTGCTGTGCTGTTGCTGTTTGCAAAGCTGCCGTC
    TTGACTCCGCCTGGTAATTAGTGTACTGATAGCGAGGTATAGAAATTAGGTTATTTGTT
    AGCGACGCAAATCCTTTCTTTTTTTTTCTTCTCCCTCTGTTCTTATCTCTTTTCTCTTCAT
    CATGGAGGAAACAGGTGGCTGTAAATTTGTCCAGAACATGTTTTCCCTAATAGCCCAAC
    AAAAAAAAAAA
    The ZM62016485 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 28):
    MLAAVMDYFSTCWGPRSRAGHRGKGSDAAGRQDGLLWYKDAGQLVTGGFSMAVVQAN
    QLLEDQSQVESGSLSLADYGPQGTFVGVYDGHGGPETSRFINDHLFNHLRRFATEHKSM
    SADVIRKAFQETEEGFLSLVIKEWSFKPQIASVGSCCLVGVICAGTLYVANLGDSRAVLGRL
    VKATGEVLATQLSAEHNACYEEVRQELQSSHPDDPRIVVLKHNVWRVKGLIQISRSIGDVY
    LKKPEYNREPLHSKFRLRETFQKPILSSEPQITEHRIQPNDQFVIFASDGLWEHLSNQEAVD
    LVQSSPRNGIARRLVKAAMQEAAKKREMRYSDLKKIDRGVRRHFHDDITVVVVFLDSDAM
    SKASWSKSPSFSLRGGGVTLPAKSLAPFSAPAQLNGTH
    cDNA sequence of ZM62051019 from corn (SEQ ID NO: 29):
    TTTTCTCTTATCCAGCTTCTTAGCATGATTCTCTTTGATCCCGGAGAGCAGCCACCGGT
    CCAACTAGTCCTTGCTGTTGGTCTGCCGGAACTTTTGATTGCTCTCCAGATCTGAGGC
    ACCTGCTGGGTGGATTCCAGGAACCCGAGCACGAAGTTGACAGGTGAGTTCTCAGGG
    AAAAAGGGGAGGAAGGAAGAGGCTGAAAGGGCGGTG
    GAGAGAGAAAGACTATAAGGGCGAGCTGAGTCCCTTGAGGATGCCGTCAAGAAACCG
    CGTGGAGAGGAGGGCGATGAGATGAGGGATGCTGGCCGCGGTGATGGACTACTTCA
    GCTCCTGCTGGGGCCCGCGATCGGGCGCCGGGCACCGGGGCAAGGGCTCCGACGC
    CGCCGGCCGGCAGGACGGTCTCCTCTGGTACAAGGACGCCGGCCAGCTCGTCACTG
    GGGAGTTCTCCATGGCCGTGGTGCAGGCCAACCAGCTCCTCGAGGACCAGAGCCAAG
    TAGAGTCCGGATCGCTCTCCCTGGCTGACCCGGGCCCACAGGGCACCTTCGTCGGCG
    TCTATGATGGCCATGGCGGCCCGGAGACGTCCCGGTTCATCAATGACCACCTCTTCAA
    CCATCTCAGAAGGTTTGCAACTGAGCACAAGTTTATGTCAGCGGACGTGATCCGGAAA
    GCTTTCCAAGCAACTGAGGAGGGCTTTCTTTCTCTAGTCAGCAAGGAATGGTCTTTGAA
    GCCTCAGATTGCTTCAGTGGGCTCCTGCTGCCTTGTTGGTGTAATCTGTGCTGGGACT
    CTCTATGTTGCAAACGTGGGCGACTCACGTGCAGTTCTTGGAAGGCTTGTTAAGGCAA
    CTGGAGAGGTTGTGGCCATGCAGTTGTCATCGGAGCACAATGCGTGCTATGAGGAAG
    TTAGACAAGAACTGCAGTCATCACATCCTGACGATCCACATATTGTGGTTCTCAAACAC
    AATGTTTGGCGAGTGAAGGGTCTCATCCAGATCTCAAGATCTATTGGAGATGTATATCT
    AAAGAAACCAGAGTACAACAGAGAACCACTTCACAGCAAGTTTCGGCTTCGAGAAACC
    TTCCAGAGGCCGACCCTTAGTTCTGAACCTCAAATTACTGAACATCGAATACAGCCGAA
    CGATCAATTTGTTATATTTGCTTCTGATGGTCTATGGGAGCACCTCAGCAATAAGGAAG
    CAGTTGACCTTGTCCAAAGTAGTCCCCGAAATGGAATCGCTCGGAGGCTAGTGAAAGC
    CGCGATGCAAGAAGCTGCCAAGAAGAGGGAGATGAGATACTCAGACCTCAAGAAGAT
    CGACCGTGGTGTGAGAAGGCATTTCCACGACGATATAACTGTCGTCGTGGTATTCCTC
    GATTCGGATGCCATGAGCAAAGCTAGCTGGAGCAAAAGCCCCTCGGTTTCTCTCCGAG
    GGGGCGGTGTCGCCCTCCCTGCGAAGTCCCTCGCACCTTTCTCAGCTCCGGCACGGC
    TGAACAGCACCTACTGAAGTTGCTACCACTCTTGAAAGGAAGAACACAGTGCAGATCT
    GCAGTGGTGAGAGAGAGAGAGAAAACAGCAACCAAGTGTAGCGTTACAGTTACACCT
    GCTGTGTTGTTGCTCTTTGCAAAACTACTGTCTAGACTCCGCCTGGTAATTAGTGTACT
    GATAGCGAGGTAAAAAAAGTTAGATTATTTGTTAGCGACACACATCCTTTCACCTTCTC
    TTCTCTCCCTCGATTCCTATCCCTTTTCTCTTCATCCTTGAGAGAACAGGTGGATGTAA
    ATTGTTCAGAACATGTTTTCCCTTATAGTCCATCATATCCCGCTTTTTTCGTGTTGAAAA
    AAAAAAAAAA
    The ZM62051019 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 30):
    MLAAVMDYFSSCWGPRSGAGHRGKGSDAAGRQDGLLWYKDAGQLVTGEFSMAVVQAN
    QLLEDQSQVESGSLSLADPGPQGTFVGVYDGHGGPETSRFINDHLFNHLRRFATEHKFMS
    ADVIRKAFQATEEGFLSLVSKEWSLKPQIASVGSCCLVGVICAGTLYVANVGDSRAVLGRL
    VKATGEVVAMQLSSEHNACYEEVRQELQSSHPDDPHIVVLKHNVWRVKGLIQISRSIGDVY
    LKKPEYNREPLHSKFRLRETFQRPTLSSEPQITEHRIQPNDQFVIFASDGLWEHLSNKEAV
    DLVQSSPRNGIARRLVKAAMQEAAKKREMRYSDLKKIDRGVRRHFHDDITVVVVFLDSDA
    MSKASWSKSPSVSLRGGGVALPAKSLAPFSAPARLNSTY
    cDNA sequence of ZM65086957 from corn (SEQ ID NO: 31):
    CTCTGTCTCCTTGGATTTGCGCTTGTGCTCGTCTGGCCGCATACTAGTATCCGCACCA
    GAGAGGAGACACCTCCGACTCCGACCTGCTCTTGCATATAGATTGGACAGAGAGTGA
    GGGAGAGAGAGAGCGCGCGCGCTGAAGGGGTGCCAAAGGGAGATTTTTTTTTTTTAAT
    CCAGCTTCTTAGCCTGACTGACTCTCTTTGATCCCGG
    AGAGCAGCCGCCAGCCCAACTAATCCTTGCTGCTGGCGCGCCGGGGCTCTGATTGCG
    CTCCAGATCTGAGGCACCTGCTCGGTGGATTCCAGGAATCCGAGCACCAACTCGACA
    GGGAGAGTTCTCAGGGTAAAGGACGACGCTTGATGCACACGGGACGGGACAACGAGT
    TGGCCGCAAGTTTTGTTTGCACACGCACACGACCCACCAGCTCACGCGTTTTTTTTTTT
    TTTTTTGCTTCTTAACTCGCTTTGATTGCATCTGTTGTTTCGGAAGGAAGAGGCTGAGA
    GCGCGTTGGAGATTTGGACTATAAGAGCGAGCGAGCGAGCGAGCTGGGTGCCTTGAG
    GACGCCGTCAAGAAACCGCGTGGAGGGGAGGGCGATGAGATGAGGGATGCTGGCCG
    CGGTGATGGACTACTTCAGCACCTGCTGGGGCCCGCGGTCTCGTGCGGGGCACAGG
    GGCAAGGGCTCCGACGCCGCCGGCCGGCAGGACGGCCTCCTCTGGTACAAGGACGC
    CGGGCAGCTCGTCACCGGGGGGTTCTCCATGGCCGTGGTGCAGGCCAACCAGCTGC
    TTGAGGACCAGAGCCAGGTGGAGTCCGGATCGCTCTCCCTGGCTGACTACGGCCCGC
    AGGGCACCTTCGTCGGCGTCTATGATGGCCATGGCGGCCCGGAGACGTCCCGGTTCA
    TCAATGACCACCTCTTCAACCATCTCAGGAGATTTGCAACTGAGCACAAGTCCATGTCA
    GCAGACGTGATCCGGAAAGCTTTCCAAGAAACTGAGGAGGGCTTTCTTTCTCTAGTCA
    TCAAGGAATGGTCTTTCAAGCCTCAGATTGCATCAGTTGGCTCCTGTTGCCTTGTTGGT
    GTAATCTGTGCTGGGACTCTCTATGTTGCAAACCTGGGCGACTCCCGTGCAGTTCTTG
    GAAGGCTTGTTAAGGCAACTGGAGAGGTTCTGGCCACGCAGTTGTCAGCGGAGCACA
    ATGCATGCTATGAAGAAGTTAGACAAGAGCTGCAGTCATCACATCCTGATGATCCACG
    TATTGTGGTTCTAAAACATAACGTTTGGCGAGTGAAGGGTGTCATCCAGATCTCAAGAT
    CTATCGGAGATGTATATCTAAAGAAACCGGAGTATAACAGAGAACCTCTTCACAGCAA
    GTTTCGGCTTCGAGAAACCTTCCAGAAGCCGATTCTTAGTTCTGAACCTCAAATTACTG
    AACACCGAATACAGCCAAACGATCAGTTTGTTATATTTGCTTCTGATGGTCTATGGGAG
    CACCTCAGCAATCAGGAAGCTGTTGACCTTGTCCAAAGTAGTCCCCGTAATGGAATCG
    CTCGGAGACTAGTGAAAGCCGCGATGCAAGAAGCTGCCAAGAAGAGGGAGATGAGAT
    ACTCAGACCTCAAGAAGATCGACCGTGGCGTGAGGAGGCATTTCCACGACGATATAAC
    CGTCGTCGTGGTGTTCCTCGACTCGGATGCCATGAGCAAAGCTAGCTGGAGCAAGAG
    CCCCTCGGTTTCTCTCCGAGGGGGCGGCGTCACCCTTCCCGCCAAGTCCCTCGCACC
    CTTCTCGGCTCCGGCACAGTTGAACGGCACCCACTGAAGCTGCTACTGCTCTTGAAAA
    GGGGCACAGTGCAGATCTGCTAGAGATGATGAGAGAAGCAGCAATCAAGTCAAGTGT
    AGCTGTTGCTCGTACACCTGCTGTGCTGTTGCTGTTTGCAAAGCTGCCGTCTTGACTC
    CGCCTGGTAATTAGTGTACTGATAGCGAGGTATAGAAATTAGGTTATTTGTTAGCGACG
    CAAATCCTTTCTTTTTTTTCTTCTTCTCTCTCTGTTCTTATCCCTTTTCTCTTCATCATGG
    AGGAAACAGGTGGCTGTAAATTTGTCCAGAACGTGTTTTCCCTAATAGCCCATCATATC
    CCGCTATTTTTCTTGTTAAAAAAAAAA
    The ZM65086957 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 32):
    MLAAVMDYFSTCWGPRSRAGHRGKGSDAAGRQDGLLWYKDAGQLVTGGFSMAVVQAN
    QLLEDQSQVESGSLSLADYGPQGTFVGVYDGHGGPETSRFINDHLFNHLRRFATEHKSM
    SADVIRKAFQETEEGFLSLVIKEWSFKPQIASVGSCCLVGVICAGTLYVANLGDSRAVLGRL
    VKATGEVLATQLSAEHNACYEEVRQELQSSHPDDPRIVVLKHNVWRVKGLIQISRSIGDVY
    LKKPEYNREPLHSKFRLRETFQKPILSSEPQITEHRIQPNDQFVIFASDGLWEHLSNQEAVD
    LVQSSPRNGIARRLVKAAMQEAAKKREMRYSDLKKIDRGVRRHFHDDITVVVVFLDSDAM
    SKASWSKSPSVSLRGGGVTLPAKSLAPFSAPAQLNGTH
    cDNA sequence of ZM68587657 from corn (SEQ ID NO: 33):
    GGACGCCGGGCAACATGTCAATGGGGAGTTCTCCATGGCTGTTGTTCAGGCAAATAAC
    TTACTTGAGGACCAGTGTCAGATCGAGTCGGGCCCACTGAGTTTTCTAGATTCTGGAC
    CATATGGCACTTTCGTTGGTGTTTACGATGGGCATGGTGGTCCAGAGACGGCCTGCTA
    TATCAATGATCATCTTTTCCAGAATCTGAAAAGTAA
    CTTGCTAACCTTTAAATCTGTGCAGTAGCACTATTCCCGTTTCTTAGCACTATATCTGCA
    TTTGGCTTTCAGTTTGCACATAAAGGAGATCATCCATTTTTTCATGGCTTGTATTTAGGA
    TTTGCATCTGAGCAGAATGCAATGTCTGCTGATGTACTGAAGAAGGCATATGAAGCTAC
    AGAAGATGGATTCTTCTCCATTGTTACCAAA
    CAATGGCCTGTAAAGCCTCAGATAGCAGCTGTCGGCTCATGCTGCCTGGTCGGTGTAA
    TTTGTGGTGGCATGCTTTATGTTGCCAATGTTGGGGATTCCCGTGTCGTTTTAGGAAAA
    CATGTTAAGGCCACTGGAGAAGTTTTGGCTGTCCAACTGTCAGCAGAACATAATGTTA
    GTATTGCGTCCGTGAGAAAAGAACTGCAGTCAATG
    CACCCAGAAGATAGGCACATTGTTGTTCTCAAGCACAATGTTTGGCGTGTTAAAGGACT
    AATTCAGGTTTGTAGATCAATTGGTGATGCATATCTCAAAAAGCAAGAGTTCAACAGGG
    AACCCCTATATGCAAAATTTCGCCTCCGTGAACCTTTTCACAAGCCAATACTAAGTTCA
    GAACCATCAATCAGTGTGCAACCACTACAACCA
    CACGACCAGTTTCTCATATTTGCATCTGATGGACTTTGGGAGCAGTTAACCAACCAAGA
    GGCAGTTGATATTGTTCGAAGTAGCCCCCGCAGTGGCTGTGCTAGGAGGCTGATAAG
    AGCGGCACTGCAAGAGGCAGCCAAGAAAAGAGAGATGAGGTACTCGGACCTCAAGAA
    GATTGACCGCGGTGTTCGCCGCCACTTCCACGACGACATAACAGTCATAGTAGTGTTC
    CTTGACTCCGGCCTCGTAAGCCAGGCGAGCACACACCGAGGTCCAACTCTTTCCTTGC
    GAGGCGGTGGCGGCAGCGCTGGCCTGCGCAGCAACACACTTGCACCTACGTGACTAT
    AAAGTGCCTGGTGGAGTGGAGGCTACTGACTGAAGGTGGTTTTCTTTCCTTGTGTCGA
    ATGTGTTATATATGTACTTGTACCAGCCAAGATCATTCATCCCCCCCCCTAAAATGGTG
    TAAAGAAGTAGGAGAGGCGCCGAAGTTCCTCACCAGCGTATCTGAATGCCCTCAATGG
    TGTCAAGTTGTGGACTCAAGTGGATAGCTTCGCTGAATCTTCTGATGATGCTCTGTGGA
    AAGCTCGAATCCTTTCCACCTGAAAAAGCAAGTAATATGTCTTCCAGTGCTGGAATTAA
    CCCCTAGTGCATATATATATGTATGAAATAATAATAAGGCAAAAGGAGGAGTAACTTAT
    TTAACTAATGCTGTGAGGTGTATTTATGTTTTGTATGTGTACTGCTTTTGACTGCTACTG
    CATCTACTGTTGTTAATTGAAAAAAAAAAAAAAA
    The ZM68587657 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 34):
    MSADVLKKAYEATEDGFFSIVTKQWPVKPQIAAVGSCCLVGVICGGMLYVANVGDSRVVL
    GKHVKATGEVLAVQLSAEHNVSIASVRKELQSMHPEDRHIVVLKHNVWRVKGLIQVCRSIG
    DAYLKKQEFNREPLYAKFRLREPFHKPILSSEPSISVQPLQPHDQFLIFASDGLWEQLTNQE
    AVDIVRSSPRSGCARRLIRAALQEAAKKREMRYSDLKKIDRGVRRHFHDDITVIVVFLDSGL
    VSQASTHRGPTLSLRGGGGSAGLRSNTLAPT
    cDNA sequence of EST390 from moss (SEQ ID NO: 35):
    ATCCCGGGTGGAGCCCTTTCAAGCCTCACGCATTCTGGATTCGCTCCCGGCTTCGAAT
    GCTTGAGTGGTTCTAAGTGATGAGATAGCGCCGTCTAGGGAGAATTTCGAATTTGCGC
    TAGAACATGGGTGGTTATTCCATCAGTGTGGCAGCGCCCACAGATATTGCAGTGAAAG
    GTTGAACACAACGACCCAAGGACAACCTGCACCTTCCAACAGTCAGCGTGAGGTGAAA
    AGATAGGCCAGTTTTCAGCTGCACATAACCTTCACTTCTGCAGGCGCAGAACACGTGC
    GGTACTGAGCAATGGGGTCCTCTAAGGCAGAAGAGAATTTGGCCTTACGGCTGGGCC
    TCACTGCAGCGTCAGCCATGGCGTCGGAGTCTGTGACCTTCCCAATCGATATCACGAA
    AACCCGCCTGCAGCTCCAAGGCGAAATGGGTGCCACAGCTGGCGCACCCAAGCGAG
    GAGCGATCAGCATGGCGATCTCTATAGGCAAGGAGGAGGGCATTGCCGGTCTTTATA
    GGGGCCTTTCTCCGGCACTTTTGCGTCATGTATTTTACACAAGCATTCGTATTGTTGCG
    TATGAAAATCTACGTACCGCCCTCAGTCATGGCGAACACCCGGAAAATCTGTCCGTTG
    CAAAAAAGGCTTTCATCGGTGGCACTTCCGGTATTATTGGGCAGGTGATAGCGAGTCC
    AGCGGATTTGGTGAAGGTGCGCATGCAAGCGGATGGGAGGCTGGTGAAGCTTGGGC
    AGCAGCCACGCTACACCGGAGTAGCTGACGCATTCACCAAGATTGCCCGAGCCGAGG
    GTGTGACAGGGCTGTGGCGTGGAGTGGGACCCAATGCTCAACGTGCCTTCCTCGTCA
    ACATGGGGGAGCTTGCATGCTACGACCAGTCGAAGCAATGGATCATAGGACGCGGCA
    TTGCTGCCGACAACATCGGAGCTCACACGCTTGCATCAGTGATGTCTGGGTTATCAGC
    TACTATTCTGAGCTGCCCTGCCGATGTGGTGAAGACCCGGATGATGAACCAAGGCGCT
    GCAGGTGCCGTGTACCGCAACTCTCTGGATTGTCTCACCAAAACCGTGAAGGCTGAAG
    GCGTGATGGCGCTGTGGAAGGGCTTCTTCCCGACGTGGACAAGGCTGGGCCCTTGG
    CAATTCGTGTTTTGGGTCTCATATGAGCAGCTCCGCCGCATCAGCGGTCT
    ATCATCCTTCTAATAAGTAAAGCCTCGCAGTTGTTTTGGGTGTGAAACTTACATGGCAT
    TCAGCTCTTACAAAGATTTCACATGCTTGAAGATTTTGAGGTGCTGTTTTTTTTATCATT
    TTTGTTCCTTCTCTTTTCTGCCTCAATTGGATGTCATAGCT
    GAGGCTATGAAGCTTAGTTTCATTGACAAATGTTTACATTTGTTAGCAATGTGTAGTAGT
    GCACTTGCGTTAACCG
    The EST390 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 36):
    MGSSKAEENLALRLGLTAASAMASESVTFPIDITKTRLQLQGEMGATAGAPKRGAISMAISI
    GKEEGIAGLYRGLSPALLRHVFYTSIRIVAYENLRTALSHGEHPENLSVAKKAFIGGTSGIIG
    QVIASPADLVKVRMQADGRLVKLGQQPRYTGVADAFTKIARAEGVTGLWRGVGPNAQRA
    FLVNMGELACYDQSKQWIIGRGIAADNIGAHTLASVMSGLSATILSCPADVVKTRMMNQGA
    AGAVYRNSLDCLTKTVKAEGVMALWKGFFPTWTRLGPWQFVFWVSYEQLRRISGLSSF
    cDNA sequence of BN51363030 from canola (SEQ ID NO: 37):
    AGAAAACAAATAAAAATCAAATCGTTACAGCAATGGGCGTCAAAAGTTTCGTGGAAGGT
    GGGATTGCCCCTGTAGTCGCCGGCTGCTCCACTCACCCTCTCGATCTCATCAAGGTTC
    GCCTTCAGCTCCACGGCGAAGCTTCCGCCGTCACTCTCCTCCGCCCAGCTCTCGCTTT
    CCACAATTCTCCCCCAGCTTTTCTGGAGACGACTC
    ATTCGGTCCCTAAAGTAGGACCCATCTCCCTCGGAATCAACCTCGTCAAAACCGAAGG
    CGCCGCCGCGCTTTTCTCCGGCGTCTCCGCCACACTCCTCCGTCAGACTCTCTACTCC
    ACCACCAGGATGGGTCTCTACGAGGTGTTGAAAAACAAATGGACTGATCCCGAGTCCG
    GTAAGCTGAGTCTCACTCGTAAAATCGCCGCGGGGCTAGTCGGTGGCGGGATCGGAG
    CCGCCGTCGGGAACCCAGCCGACGTGGCGATGGTAAGGATGCAAGCCGACGGGAGG
    CTTCCCGTGGCAGAGCGTCGTAACTACGCGGGCGTAGGAGACGCGATCAAGAGGATG
    GCGAAGCAAGAAGGCGTGGTGAGCCTGTGGCGCGGCTCGGCTCTGACGATCAACAG
    GGCGATGATAGTGACGGCGGCGCAGCTCGCGTCGTACGATCAGTTCAAGGAAGGGAT
    GGTGGAGAGCGGGGGGATGAAAGATGGGCTCGGGACTCACGTGGTGGCGAGCTTCG
    CGGCGGGGATCGTGGCGGCTGTTGCGTCGAATCCGGTGGATGTGATAAAGACGAGG
    GTGATGAATATGAAGGTGGATGCGCGTGGTGGGGAGGCTCAGTACAAAGGCGCGTGG
    GATTGTGCGGTGAAGACGGTTAGAGCTGAAGGACCGATGGCTCTTTATAAAGGGTTTG
    TTCCTACGGTTTGCAGGCAAGGACCTTTCACTGTTGTGCTCTTTGTTACGTTGGAGCAA
    GTCAAGAAGCTGCTTCGTGATTTTTGATTATCATTTGAAGGTTATGATGATGAGGACGA
    CTAAGAATAAGAATGCTAGTAGTATTGATTTGATAGGGATTTTTCGTATTGGGTTATTCA
    TTTTCG
    The BN51363030 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 38):
    MGVKSFVEGGIAPVVAGCSTHPLDLIKVRLQLHGEASAVTLLRPALAFHNSPPAFLETTHSV
    PKVGPISLGINLVKTEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKNKWTDPESGKLSLTR
    KIAAGLVGGGIGAAVGNPADVAMVRMQADGRLPVAERRNYAGVGDAIKRMAKQEGVVSL
    WRGSALTINRAMIVTAAQLASYDQFKEGMVESGGMKDGLGTHVVASFAAGIVAAVASNPV
    DVIKTRVMNMKVDARGGEAQYKGAWDCAVKTVRAEGPMALYKGFVPTVCRQGPFTVVLF
    VTLEQVKKLLRDF
    cDNA sequence of BN42986056 from canola (SEQ ID NO: 39):
    TCTAAAAAAACTTTTTGTCTGAACGGCATATGTCTCAGAGACCTCAAGTTCCTCATTCTT
    CTTCTATAGCTTTCGGTCTCCATTCTCATCTCCTAATCTCCAGTGAGATCAGCTCCAATT
    CCAACTGGTCTCTCTAAGAAAAAAATAATCAAACCTTTTCAAAATTTTCTCTCGGATTTT
    CTCGGAATAAAAATCTAACCTTTCTGACTTTTTTGATTTTCGATTTGATAAAAACAAGAA
    ATGGGTCTTAAGGGTTTCGCTGAAGGAGGCATCGCATCGATCGTAGCGGGATGTTCG
    ACCCACCCGCTTGATCTAATCAAGGTCTGAATGCAGCTCCAAGGGGAATCAGCCTCGA
    TTCAGACAAATCTCCGACCAGCTCTTGCTTTCCAGACTTCCTCCGCCGTTCACGCGCC
    TTCGCCTCCTCCGCGCGTGGGTATAATCACCATCGGATCTCGCATCATCAGACAAGAA
    GGCACGTGCACTCTCTTCTCCGGCATCTCCGCCACCTCCGCCACCGTTCTCCGCCAG
    ACTCTCTACTCGACGACTCGCATGGGTCTATACGACATCCTGAAAACCAAATGGACCG
    ACCCGGAAACCAAAACCATACCTTTGACCCGCAAACTCGCCGCCGGGTTCATCGCCG
    GAGGTATCGGCGCCGCCGTCGGGAACCCGGCGGATGTCGCCATGGTGCGCATGCAA
    GCCGACGGGAGGCTCCCGGTGGTCGACCGGAGGAACTACAAGAGCGTTTTGGACGC
    GATCGCGCAGATGGTTCGCGGCGAAGGCGTCACGTCGCTGTGGAGAGGTTCGTCGAT
    GACGATCAACAGAGCGATGCTCGTGACG
    GCGTCGCAGCTGGCTACGTACGACTCGGTGAAAGAGACGATTTTGGAGAAAGGGTTG
    ATGAGGGACGGGCTCGGGACTCACGTGACGTCGAGCTTCGCGGCGGGGTTTGTGGC
    TTCGGTCGCGTCGAACCCCGTGGATGTGATCAAGACGAGAGTGATGAATATGAAAGTG
    GAGGCGGGGAAAACGGCGCCGTATAAGGGAGCGGTTGATTGCGCGTTGAAGACGGT
    GAGAGCGGAAGGGATCATGGCTTTATACAAAGGGTTTCTGCCGACGGTGTCGAGACA
    AGCACCGTTCACGGTGATTATGTTTGTGACACTTGAACAAGTTAAGAAGGTGTTCAAGG
    ACTTTGACTTTTGAGACAAGAGTTAAAGATGATGGTGGCGATAATTTGCTTTAAACTAAA
    TAAATTTTGTTTTTTTTTATTGTATTTTCTTT
    The BN42986056 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 40):
    MQLQGESASIQTNLRPALAFQTSSAVHAPSPPPRVGIITIGSRIIRQEGTCTLFSGISATSAT
    VLRQTLYSTTRMGLYDILKTKWTDPETKTIPLTRKLAAGFIAGGIGAAVGNPADVAMVRMQ
    ADGRLPVVDRRNYKSVLDAIAQMVRGEGVTSLWRGSSMTINRAMLVTASQLATYDSVKET
    ILEKGLMRDGLGTHVTSSFAAGFVASVASNPVDVIKTRVMNMKVEAGKTAPYKGAVDCAL
    KTVRAEGIMALYKGFLPTVSRQAPFTVIMFVTLEQVKKVFKDFDF
    cDNA sequence of BN49389066 from canola (SEQ ID NO: 41):
    CGACGATTTCGTTTAATATAAACATCACCAAGTGAATCTCTCCGCCTCTCTCTCTCTTTC
    TCTGCGGAATCTCTTCGTCTCGTTGCGTTCGAGAGTTCCGTACGATTCCCAACAAGAA
    AGGGAAGAGATGGCGGAGGAGAAGAAAGTAGCTCCGATTGGTATCTGGACTGCCGTG
    AAGCCTTTCGTCAATGGCGGTGCCTCTGGTATGCT
    CGCCACTTGCGTTATCCAGCCTATTGACATGATCAAGGTGAGGATTCAACTAGGTCAG
    GGATCTGCAGCTAGTGTGACCACCACCATGTTGAAGAATGAAGGTATCGGTGCCTTCT
    ACAAGGGATTATCAGCTGGTTTGCTGAGGCAAGCAACTTACACCACAGCTCGTCTTGG
    ATCATTCAAGATGCTGACTGCGAAAGCAAGCGAGGCTAACGATGGGAAGCCACTACC
    GCTGTATCAAAAAGCTCTATGTGGTCTGACAGCTGGTGCTATCGGTGCCTGCGTTGGT
    AGTCCAGCCGATTTAGCGCTTATCAGAATGCAAGCTGATAACACTTTGCCGTTAGCTCA
    GCGCAGGAACTATACCAACGCCTTCCATGCGCTTTACCGTATTAGCGCTGATGAAGGA
    GTTTTGGCGCTTTGGAAAGGTTGTGGGCCAACTGTGGTCAGAGCAATGGCTTTGAACA
    TGGGGATGCTTGCGTCTTATGATCAAAGTGCTGAGTATATGAGAGATAATCTTGGTCTT
    GGGGAGACATCCACAGTCGTAGGAGCAAGTGCTGTTTTGGGATTCTGCGCTGCGGCT
    TGCAGTCTTCCATTTGACTTTGTCAAAACACAGATCCAAAAAATGCAACCCGACGCTCA
    GGGTAAATATCCATACACTGGTTCGCAGGACTGTGCGATGCAAAACAGGAGGACCTTT
    GAAATTCTACACAGGCTTTCCGGTATACTGCGTCAGGATCGCCCCTCACGTCATGGTG
    ACATGGATCTTCCTGAACCAGATTACAAAGTTCCAAAAGAACATTGGGATGTGATCTTC
    AAGCAAACCTTATGAAGTGCGCGGTG
    AAAATATGATGAGAAGAATTCATTTGCTTTTTAATCATATACATGATTAG
    The BN49389066 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 42):
    MAEEKKVAPIGIWTAVKPFVNGGASGMLATCVIQPIDMIKVRIQLGQGSAASVTTTMLKNE
    GIGAFYKGLSAGLLRQATYTTARLGSFKMLTAKASEANDGKPLPLYQKALCGLTAGAIGAC
    VGSPADLALIRMQADNTLPLAQRRNYTNAFHALYRISADEGVLALWKGCGPTVVRAMALN
    MGMLASYDQSAEYMRDNLGLGETSTVVGASAVLGFCAAACSLPFDFVKTQIQKMQPDAQ
    GKYPYTGSQDCAMQNRRTFEILHRLSGILRQDRPSRHGDMDLPEPDYKVPKEHWD-
    VIFKQTL
    cDNA sequence of BN51339479 from canola (SEQ ID NO: 43):
    CTTTCTCCGCCTATCTCTTTCTCTCCGCGGATTCTCTTCTTCTCGTTTCGACTCCGTAC
    GATCCCCAAAGAAAAAAAGAGATGGCGGAAGAGAAAAAAGTAGCTCCGATTGGTGTCT
    GGAATACCGTGAAGCCCTTCGTCAATGGCGGTGCCTCCGGTATGCTCGCCACTTGCG
    TTATCCAGCCGATCGACATGATCAAGGTGAGGATTC
    AACTAGGTCAGGGATCTGCAGTCAGTGTGACCAAGAACATGTTGAAGAATGATGGTAT
    TGGTGCTTTCTACAAGGGATTGTCTGCTGGTTTGCTAAGGCAAGCAACTTACACCACA
    GCCCGTCTTGGATCCTTCAAGATGCTGACTGCAAAAGCAATTGAGGCTAACGATGGGA
    AGCCGCTACCTCTGTACCAGAAGGCTCTATGTGGTC
    TGACAGCTGGTGCAATCGGTGCTTGCGTTGGTAGTCCAGCTGACTTGGCGCTTATCAG
    AATGCAAGCTGATAACACCTTGCCGTTAGCTCAGCGCAGGAACTATACCAATGCCTTC
    CATGCGCTTTACCGTATTAGCGCTGATGAAGGAGTTTTGGCACTTTGGAAAGGTTGTG
    GTCCTACTGTGGTCAGAGCTATGGCTTTGAACATGG
    GAATGCTTGCTTCTTATGATCAAAGTGCTGAGTACATGAGAGATAATCTCGGTCTTGGG
    GAGACTTCTACGGTCGTAGGAGCAAGTGCTGTTTCTGGATTCTGCGCTGCGGCTTGCA
    GTCTTCCATTTGACTTTGTCAAAACTCAGATCCAGAAGATGCAACCTGACGCTCAGGG
    GAAGTATCCATACACGGGTTCGCTTGACTGTGCCA
    TGCAAACCTTGAAGTCAGGAGGACCTCTTAAATTCTACACAGGTTTCCCTGTTTACTGC
    GTCAGGATCGCCCCTCACGTCATGATGACATGGATCTTCCTGAACCAGATTACAAAGT
    TTCAAAAGACCATTGGTCTGTGAGCTTCAAGCATTGTGAAGTGCGCGCTGAGAATAAG
    TTGAAAACGAAAACGCAATTGGAATTGTGTTAGAT
    TTGCTTTTTATTCAATATACATGATCGCATGCCTTAACGCATTATTTGAAGTGTTGGAGA
    CTTTA
    The BN51339479 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 44):
    MAEEKKVAPIGVWNTVKPFVNGGASGMLATCVIQPIDMIKVRIQLGQGSAVSVTKNMLKND
    GIGAFYKGLSAGLLRQATYTTARLGSFKMLTAKAIEANDGKPLPLYQKALCGLTAGAIGACV
    GSPADLALIRMQADNTLPLAQRRNYTNAFHALYRISADEGVLALWKGCGPTVVRAMALNM
    GMLASYDQSAEYMRDNLGLGETSTVVGASAVSGFCAAACSLPFDFVKTQIQKMQPDAQG
    KYPYTGSLDCAMQTLKSGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQITKFQKTIGL
    cDNA sequence of ZM57651070 from corn (SEQ ID NO: 45):
    CTAGCACGTGAAAATTCCTTCGGCTCCAGTTATTACGGAGGATTAGGTTGGTGAACTG
    GTGACTGGAGCTGGAATCGCATTTCTTGCTCTTTGGTCTCTCCAGAATCATCCTCCGG
    CCAGCCGTTCTTGGAATCCTCCCGAGATTCGCTTGCCCGCCCTTTTCTTTTCAAGTGG
    ATCTGAACTTGGGAGGGAACCCCGATGCAGCCGCGG
    TACGGAGAAGCACGACAACCGCTGCCGGGGCGGTACGCGCTGTACCACTTCGGCAC
    CAGCGGCGCCGCCGTCGCCGCCGCCACCGCCGTGACCCATCCGTTCGATGTTATCAA
    AGTCAGGCTTCAAATGCAGCTTGCTGGGCAAAGAGGAAACTTAGTTGGAATGGGAACA
    ATATTTACACAAATGGTTGAAAGGGAAGGGACTCGGTCACTCTACCTGGGACTTGCAC
    CAGCGTTGGCGAGAGCTGTTGTCTATGGTGGCCTTCGGTTTGGACTGTATGAGCCCTG
    CAAGCATGTCTGCAGTTATGCATTTGGTTCAACAAACTTTGCTTTTAAATTTGCATCTGG
    AGTCATTGCTGGGGGCCTTGCAACTGCTTTAACAAATCCCATGGAAGTTTTGAAGGTG
    AGGCTGCAGATGAGTAAAAGCAGTACCAGTACAATAAGAGAGATGAGAAAAGTTATAG
    CGCACGAAGGGTTTAAAGCACTTTGGAAAGGAGTCGGCCCAGCAATGACAAGAGCAG
    GTTGCCTTACTGCATCACAAATGGCGACTTACGATGAGGCCAAACAGGCCTTAATGAA
    GTGGACACCACTTGAAGAAGGTTTTCAGTTACATCTCATCTCGAGTTTCATAGCTGGAA
    CAGCTGGTACTCTTGTGACCTCACCTGTAGACATGATCAAAACACGGTTAATGCTGCAA
    CAGGAGTCCAAAGGCGCCAGAGTATACAGGAACGGATTCCATTGTGCTTCCCAGGTTG
    TGGTGACAGAGGGTGTGAAATCACTTTATAAAGGTGGATTTGCCACATTCGCGAGAGT
    AGGCCCTCAGACAACGATTACCTTTATCGTGTGCGAGAAACTGCGCGAACTTGCAGGA
    ATGACTGCCATCTAGTGCCACCCCAAATTGCATAATGTGTGGGGTCCAACGGTTGAAC
    AGCATACTCTACCCGAGTTTTCACACCATTCTTTATTCACTATTCATGATGAGAAGGGA
    GAAGATAAGCACCCACTGGGATGTCTAAGGATTGGGAAGCCCAGAGCTCCTTCAGATT
    TATCATACCTGATTTGAAATTTCGAAATAGCGTGATTGTTCTTATGTTTGCTCTAAGACT
    TACTCATCATATCTCCAATCTCATCTTGTATTTCAAACTACACTCTACAAACAATACAGT
    CTGTAGTGTAAAAACATTATTTTGGGTGACCATATGGGTAACCTGCTGTA-
    CAAAAAAAAAA
    The ZM57651070 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 46):
    MQPRYGEARQPLPGRYALYHFGTSGAAVAAATAVTHPFDVIKVRLQMQLAGQRGNLVGM
    GTIFTQMVEREGTRSLYLGLAPALARAVVYGGLRFGLYEPCKHVCSYAFGSTNFAFKFAS
    GVIAGGLATALTNPMEVLKVRLQMSKSSTSTIREMRKVIAHEGFKALWKGVGPAMTRAGC
    LTASQMATYDEAKQALMKWTPLEEGFQLHLISSFIAGTAGTLVTSPVDMIKTRLMLQQESK
    GARVYRNGFHCASQVVVTEGVKSLYKGGFATFARVGPQTTITFIVCEKLRELAGMTAI
    cDNA sequence of ZM62073276 from corn (SEQ ID NO: 47):
    GCCGCCTCTCCTACTGCATCTCCCTCGCTCTCGTCGCCTCGTTCGCTTCGCCTCCGCC
    CCGCCCCGCCCCGAGCAGAGCGCAGCCCTATCCGGAGCTGGGATGGCGGACGCGAA
    GCAGCAGCAGCAGCAGCAGCAGCAGCCACAGCAGGCGGCAGCGGCAGCCACCGGC
    GTGTGGAAGACGGTCAAGCCCTTCGTTAACGGCGAGGCCTCTGGGATGCTCGCGACC
    TGCGTCATCCAGCCTATCGACATGGTCAAGGTGAGGATCCAGTTGGGTGAGGGCTCT
    GCTGGTCAGGTCACAAGGAACATGCTTGCAAATGAGGGTGTCCGTTCTTTCTACAAGG
    GTTTGTCCGCCGGATTGCTGAGGCAAGCGACGTACACGACTGCTCGTCTTGGATCCTT
    TAGGGTTCTAACTAACAAAGCAGTTGAAAAGAATGAAGGGAAGCCATTGCCTCTATTTC
    AGAAAGCTTTTATTGGTCTGACTGCTGGTGCAATTGGTGCTTGTGTTGGTAGTCCTGCT
    GATCTGGCACTCATTAGAATGCAAGCCGATTCGACCCTGCCAGTTGCACAACGACGCA
    ACTATAAGAATGCTTTCCATGCACTCTACCGTATCAGTGGTGATGAGGGAGTCCTTGC
    GCTTTGGAAGGGTGCAGGTCCAACTGTGGTGAGAGCTATGGCACTCAATATGGGTATG
    CTTGCTTCCTATGACCAGAGTGTCGAGCTATTTAGGGACAAATTTGGCGCAGGAGAAA
    TTTCTACTGTTGTTGGAGCCAGCGCTGTTTCTGGATTCTTTGCCTCAGCATGCAGTTTG
    CCCTTTGACTATGTGAAGACACAGATTCAGAA
    GATGCAACCTGATGCGAATGGCAAGTACCCATACACAGGGTCTTTGGACTGTGCTGTG
    AAGACCTTCAAGAGCGGTGGCCCATTCAAGTTCTACACTGGTTTCCCGGTGTACTGCG
    TCAGGATTGCACCCCATGTCATGATGACCTGGATATTCTTGAATCAGATCCAGAAGTTT
    GAGAAGAAGATCGGCATATAGGATTCCCATCGGAC
    GGATACAGGGTTGACAGTTCTATGCTATTACTGCTTGACTCTGTAATAACATTCCAGCT
    GCTTTCGCACCATGGTAGTTGGTTTTGGTAGAGACAAGTCTGTTACAATTTTTTACCTTA
    GCTTTCCAATTATTGTGTTGCAATAATCGAATTAATTGTTGCTGGGGGATTTTTTTGGGG
    GGTTGGGAGGGTGGCATGCTTTTGTTGGCTG
    GGATGTAGCCATAAGGAGAGGGGGATACTGCCTAGTTATGTCATTGAATGGAATTGGA
    CCATATTTTATACAGATTTTTACCTTCAAAAAAAAAAAAAA
    The ZM62073276 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 48):
    MADAKQQQQQQQQPQQAAAAATGVWKTVKPFVNGEASGMLATCVIQPIDMVKVRIQLGE
    GSAGQVTRNMLANEGVRSFYKGLSAGLLRQATYTTARLGSFRVLTNKAVEKNEGKPLPLF
    QKAFIGLTAGAIGACVGSPADLALIRMQADSTLPVAQRRNYKNAFHALYRISGDEGVLALW
    KGAGPTVVRAMALNMGMLASYDQSVELFRDKFGAGEISTVVGASAVSGFFASACSLPFDY
    VKTQIQKMQPDANGKYPYTGSLDCAVKTFKSGGPFKFYTGFPVYCVRIAPHVMMTWIFLN
    QIQKFEKKIGI
    cDNA sequence of EST257 from moss (SEQ ID NO: 49):
    CCCGGGGATTCAGCAGTACTTCACAAGAAGAATAGCATGGTGCGTGCAGATCTTGTCA
    ACCTTGCGGACTTAGATACTGCTCTAAACAGAGTTCATAATAAGCTACCTAATTCCATA
    GAAACAGCTAGTGCAGAGCCTCCTGCTCCTCCAGAAGAATGGGAAATAAATCCTCGAG
    AGATCACTTTGAAGCATATGATTGCGCGTGGCACCTTTGGGACTGTCCACAAAGGAGT
    GTACAAAGGTCAGGATGTCGCAGTTAAGCTACTTGAGTGGGGCGAGGAGAATACCAT
    GAAGAAAACAGAGGTTCAATACTACAGAAACCAATTCAGACAAGAGGTTGCTGTGTGG
    CATAAACTCGACCACCCTAATGTCACGAAGTTCATCGGAGCCTCGATGGGGAACTCAG
    ATTTGCGGATTCCCTCAGCCGTGGATGGTGATGATGGATTCCATCATGTGCCGAACAA
    TGCTTGTTGTGTTGTCGTTGAGTACCTTGCAGGCGGGACTCTTAAAGATCATCTCATTC
    GCAGCCGGCGGAAAAAACTCTCGTACAAGGTGGTCGTGCAATTAGCCTTGGATGTTTC
    TAGAGGGCTTGCATACCTCCATTCTCAGAAGATCGCTCATCGTGACGTGAAGACAGAG
    AACATGTTGCTCGATAAACAGATGAGGGTCAAAATTGCAGATTTCGGAGTTGCACGAG
    TGGAGGCATCCAATCCCAAGGACATGACTGGTGATACTGGTACCCCAGGATACATGGC
    TCCGGAGATTCTCGACGGCAAGCCCTACAACAAGAAGTGCGATGTGTACAGCTTCGG
    GATCTGTTTGTGGGAAGTTTATTGCTGCGACATGCCGTACTTGGACCTCTCCTTTGCG
    GACATGACATCGGCAGTTGTGCATCAGAATTTGAGACCCGAGGTGCCCAAGTGCTGC
    CCTCAGGGACTCGCGGATATCATGAGGCAGTGTTGGGATGCAAATCCTGAGAAACGG
    CCTGCCATGGCTGATGTGGTGCAGATGCTGGAGGCTCTAGACACCTCCAAAGGTGGA
    GGTATGATACCAACAGACGCCCAGCCGCATGGGTGTCTCTGTTTTGGGAGATTCAAGG
    GCCCATAGCACGTTTTTGGTTTTTTTTTTCCTTAATTGTGGTTTTACATTTTATTTATATTT
    TTCCCTTTTTTAATGTAGGGATGACATGATAATAAGTGTGCAAACATTTTGTTGTCTCCC
    CTGGTTTCGTTTCAAGCGTAGCTGCTTGACTTGCAATTTCAGTAACCTGGTGCAGGAC
    CCGTTAAC
    The EST257 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 50):
    MVRADLVNLADLDTALNRVHNKLPNSIETASAEPPAPPEEWEINPREITLKHMIARGTFGTV
    HKGVYKGQDVAVKLLEWGEENTMKKTEVQYYRNQFRQEVAVWHKLDHPNVTKFIGASM
    GNSDLRIPSAVDGDDGFHHVPNNACCVVVEYLAGGTLKDHLIRSRRKKLSYKVVVQLALD
    VSRGLAYLHSQKIAHRDVKTENMLLDKQMRVKIADFGVARVEASNPKDMTGDTGTPGYM
    APEILDGKPYNKKCDVYSFGICLWEVYCCDMPYLDLSFADMTSAVVHQNLRPEVPKCCPQ
    GLADIMRQCWDANPEKRPAMADVVQMLEALDTSKGGGMIPTDAQPHGCLCFGRFKGP
    cDNA sequence of LU61665952 from linseed (SEQ ID NO: 51):
    AGGGTGATCACGAGGGAGGTATGAATTCTAAGGTGAAGGGAAATGGAAGTGTTAGTA
    GAAAAGATATGATTTTTCGAGCGGATCGAATCGATTTGAAGATCCTGGATGTACAGCTA
    GAGAAGCACCTGAGTAGGGTGTGGTCGAGGAACACCACAGACAACGCTAAGCCTAAA
    GAAGAGTGGGAGATTGATTTGTCTAAGTTGGACATCAAAACCCAGATAGCTCGTGGTA
    CTTATGGCACTGTTTATAAAGGCACTTATGATAATCAAGATGTTGCAGTGAAAGTGTTG
    GATTGGGGGGAAGATGGTATGACTACAGTATCTGAAGCTGCTTCTCTTCGAGCATCAT
    TTCGTCAAGAGGTTGCTGTTTGGCATAAGCTTGACCATCCTAATGTTACCAAATTCGTT
    GGAGCATCGATGGGAACTTCAAATCTCAAGGTTTCAAATAATAAATCTGATGGTCAGCA
    TACTGCTAGAGCATGTTGTGTTGTGGTTGAGTATCAACCTGGTGGAACACTAAAGCAG
    TACTTGATAAGAAATAGGCGAAAGAAACTTCCTTATAAAGTTGTAATACAACTTGCTTTG
    GATCTCTCTAGGGGTTTGAGTTACCTACATTCGAAGAAAATTGTGCACCGTGATGTGAA
    GTCGGAAAACATGTTGCTTGATAATCATAGAAATCTTAGGATTGCGGATTTTGGTGTTG
    CTCGAGTCGAAGCTCAAAATCCAAGTGATATGACTGGTGAAACTGGTACCCTTGGATA
    CATGGCACCTGAGGTCCTTGATGGCAAGCCATATAACAGAAGGTGTGATGTTTATAGC
    TTCGGCATATGTTTATGGGAAATCTATTGTTGTGATATGCCATATCCAGATCTTAGCTTT
    GCTGATGTGACGTCCGCGGTTGTTCGACAAAACTTGAGGCCGGAGATTCCGAGATGTT
    GTCCAAGTTCACTAGGAAGCATCATGAAGAAATGTTGGGATGCACAATCAGAGAACCG
    TCCAGAAATGGCTGAAGTGGTGAAGATGTTGGAAGCCATTGATACAAGTAAAGGAGGA
    GGAATGATCCCTGAAGACCAGAACCCTGGTTGTTTCTGCTTCGCCCCAACCCGTGGCC
    CTTAAACCCCCTTATTAATTTACTCCCCAAACAGTCCTCATCCATCTATGTGTGCACAAA
    TTTCAATTTCTTTATATTTGAGTTGTTTTCTTTGTTTATCATTTTCTTGTGTTCTTCACTTC
    TGCACATATTTTGATTTTGAACTACCTAAAGGGAGTGAAAGGATTAATGTTATAAGTAAA
    AAAAAAAAAAAA
    The LU61665952 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 52):
    MNSKVKGNGSVSRKDMIFRADRIDLKILDVQLEKHLSRVWSRNTTDNAKPKEEWEIDLSKL
    DIKTQIARGTYGTVYKGTYDNQDVAVKVLDWGEDGMTTVSEAASLRASFRQEVAVWHKL
    DHPNVIKFVGASMGTSNLKVSNNKSDGQHTARACCVVVEYQPGGTLKQYLIRNRRKKLP
    YKVVIQLALDLSRGLSYLHSKKIVHRDVKSENMLLDNHRNLRIADFGVARVEAQNPSDMTG
    ETGTLGYMAPEVLDGKPYNRRCDVYSFGICLWEIYCCDMPYPDLSFADVTSAVVRQNLRP
    EIPRCCPSSLGSIMKKCWDAQSENRPEMAEVVKMLEAIDTSKGGGMIPEDQNPGCFCFAP
    TRGP
    cDNA sequence of TA56863186 from wheat (SEQ ID NO: 53):
    AGCACTGACAACTACAACCTCGCTGGTGGCTCCGTTACCATGTCAGTGGACAACAGCA
    GCGTGGGCTCGAACGAGTCCCGCACCGTCATACTTAAGCACCCGGGCCTCCGTGATG
    CTCCAACCGCAAGCTACTCGGTTGGCAACAGTGTCTTTCGTCCCAACCGTGTGGCTGC
    GCACACCCTAAATGAAGATGCATTGGCCAGGGTTCTGATGGACCCAAATCATCCAACA
    GAGATACTTAGCAAGTACCAGCAGTGGGCCATTGATCTGGGGAGGTTGGATATGGGG
    GTTCCCTTTGCACAGGGAGCCTTTGGGAAGCTGTACCGGGGAACATATATTGGAGAAG
    ATGTTGCCATTAAGCTGCTGGAGAAGCCTGACAATGATATCGAGAGAGCACAATCGTT
    GGAACAGCAGTTTGTGCAAGAAGTTATGATGTTATCTACCCTAAGGCACCCAAATATAG
    TAAGATTTATAGGGGCTTGCAGGAAGTCAATTGTGTGGTGCATTATTACTGAGTATGCA
    AAAGGTGGCTCAGTCAGGCAGTTCCTGGCAAAAAGGCAGAACAAGTCGGTACCTTTGA
    GGCTGGCTGTCAAACAAGCATTGGATGTAGCGAGGGGAATGGCGTATGTGCATGCTC
    TGGGATTTATCCATAGGGACCTGAAGTCGGATAATCTTCTAATTGCAGCAGACAGATCC
    ATTAAGATTGCTGACTTTGGAGTTGCTCGAATTGAAGTGAAAACAGAGGGGATGACAC
    CAGAGACAGGAACCTACCGCTGGATGGCACCGGAAATGATCCAGCACAGGCCTTATG
    ATCATAAGGTTGATGTCTACAGCTTTGGGATTGTCTTGTGGGAGCTTATAACTGGCATG
    CTTCCTTTCACAAACATGACAGCTGTTCAGGCGGCTTTTGCTGTTGTAAATAAGGGTGC
    TCGTCCAGCGATCCCACATGACTGCCTGCCTTCCCTAACCCACATCATGACGCGCTGT
    TGGGATGCAAACCCTGAAGTTCGCCCACCATTCACCGAGATCGTCTGCATGCTTGAGA
    ACGCCGAGATGGAGGTCGTGAGCCATGTCCGTAAAGCGCGCTTCCGCTGCTGCGTTG
    CTGAACCCATGACCACCGACTGAAACTAAAGCAGGTTAGACTATCGCAGCGGGCATTA
    GGGAAGAAAACAGGTAAGGATGAAGAAAAGAGGCAATGCCAATGTGTTCATCGTTGTC
    AGTGCGTGGGGTCTGTGTGCCTTTACCAGTGCGCATTCTGTCTTGTGTAAGTTGCACA
    CCTCAAGTAAAAGTAATTTCGTATAGATGTTGCCTTGTATGCTAACAAAGACCTAATGG
    AGCTTTTCCGTGTTAATAATATCCGCTTGCTCTTGTACTCGTGCAAGTTTGTGCCAAAA
    AAAAAAAAAAA
    The TA56863186 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 54):
    MSVDNSSVGSNESRTVILKHPGLRDAPTASYSVGNSVFRPNRVAAHTLNEDALARVLMDP
    NHPTEILSKYQQWAIDLGRLDMGVPFAQGAFGKLYRGTYIGEDVAIKLLEKPDNDIERAQSL
    EQQFVQEVMMLSTLRHPNIVRFIGACRKSIVWCIITEYAKGGSVRQFLAKRQNKSVPLRLA
    VKQALDVARGMAYVHALGFIHRDLKSDNLLIAADRSIKIADFGVARIEVKTEGMTPETGTYR
    WMAPEMIQHRPYDHKVDVYSFGIVLWELITGMLPFTNMTAVQAAFAVVNKGARPAIPHDC
    LPSLTHIMTRCWDANPEVRPPFTEIVCMLENAEMEVVSHVRKARFRCCVAEPMTTD
    cDNA sequence of ZM62026837 from corn (SEQ ID NO: 55):
    CGCGCGGCCAAACTCCTGTTCTTCCACCTGCTGGCTGCTCCTGCCTCCCCTGCGCCC
    CAAACCCACCCGCCTCGCCGTCCCCGCAGGCCGCAGCCTGCTCTCGGCTCCCGCCG
    CCGTCTACCGCGTCCTGCGGCTGCGGTGTTGCGTCACCTCGGGTTCGCCTTAACTTC
    CACAATCCTCGCCGTCCTGGTGCTCCGCCGCCCCTCCCTT
    TGTACTCGCGCTGGAGCTGCAGATCCACCGCGACCTGGCGACCAATTCCTCCTCCCG
    CTGAAGAATTGGCGACCTTGGCCTCCGCCCCCGCGGCGCGGAGGAGTCAACTGTGGT
    AGCAACCACCGCGGAGGCTGCAAGCCTTCGGTAAGGGAGGAAAGTTGACTTGTTGGA
    AGCCGGTCCAGGGCCGCGATGACGTCGACCGCCGCCGGCGCGTCGTCGTCGGCGG
    CGAAGAGCGAGTCCTACCTGCGGGCCGACAAGATCGACCTCGAGAGCCTGGACATCC
    AGCTGGAGAAGCAGCTGGCCAAGACCTGGGAGAAGCACAAGGGGTCGTACAACCAG
    GGGCCCAGGGAGGACTGGGAGATCGACCTCGCCAAGCTCGAGATTCGCTACGTCATA
    GCGCAGGGCACCTACGGCACGGTGTATCGCGGCACGTATGATGGGCAGGACGTCGC
    AGTAAAACTATTGGATTGGGGTGAAGATGGCTTTGCGTCAGAAACTGAAACTGCCACA
    CTGCGAGCATCATTTAAGCAGGAGGTTGCTGTCTGGCATGAGCTCAACCATCCGAATG
    TTACAAAGTTTGTTGGTGCATCAATGGGTACTACAGACCTTAAGATTCCAGCCAATAGT
    TCTAACAGTGGTGGGCGCACTGAGCTGCCGCCAAAAGCATGTTGTGTTGTGGTCGAAT
    ATCTCGCTGGAGGATCACTGAAGCAGTATTTAATAAAGAACAGGCGAAGGAAGCTTGC
    ATACAAGGTTGTTGTTCAGATAGCACTGGATCTTGCCAGAGGATTGAACTATCTACATT
    CAAGAAAGATAGTACATCGGGATGTAAAAACTGAAAATATGCTGCTCGATACACAGCG
    AAACCTTAAGATTGCTGATTTTGGTGTTGCTCGTGTTGAGGCTCAGAATCCAAAGGACA
    TGACAGGCGCGACTGGGACACTTGGCTACATGGCCCCAGAGGTGCTTGAAGGCAAGC
    CATACAACAGAAAGTGTGATGTCTACAGTTTTGGCATATGCTTATGGGAAATATACTGC
    TGTGACATGCCATATCCAGACCTCAGTTTTGCAGACGTCTCGTCCGCCGTCGTTCACC
    AGAACCTGCGGCCTGACATCCCTCGCTGCTGCCCAAGCCCAATGGCGAACATCATGC
    GGAAGTGCTGGGACGCAAACCCGGATAAGCGCCCTGACATGGACGACGTGGTGCGG
    TTCCTGGAGGCCCTCGACACGAGCAAGGGCGGTGGCATGATACCAGAAGGCCAGGC
    AGGCGGGTGCTTGTGTTTCTTCAGAGCCCGTGGTCCTTAGAACCAACCAACCCTTTCC
    AGCCATCCTCTACTTGTCTCTGCCATACTACAGTATTGGAGCCAGATGTAGGCCTTTGT
    TGTTCATCGGATAGGGGATTGCAGATAACTTGATGACAATCTTTGTGATTGGTTGACAC
    TTGTTATACGTTCTATAGTGATGTGAATACCAGTGAGGAGTCCATAATACAGAGTGAAA
    AAAAAAA
    The ZM62026837 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 56):
    MTSTAAGASSSAAKSESYLRADKIDLESLDIQLEKQLAKTWEKHKGSYNQGPREDWEIDLA
    KLEIRYVIAQGTYGTVYRGTYDGQDVAVKLLDWGEDGFASETETATLRASFKQEVAVWHE
    LNHPNVTKFVGASMGTTDLKIPANSSNSGGRTELPPKACCVVVEYLAGGSLKQYLIKNRRR
    KLAYKVVVQIALDLARGLNYLHSRKIVHRDVKTENMLLDTQRNLKIADFGVARVEAQNPKD
    MTGATGTLGYMAPEVLEGKPYNRKCDVYSFGICLWEIYCCDMPYPDLSFADVSSAVVHQN
    LRPDIPRCCPSPMANIMRKCWDANPDKRPDMDDVVRFLEALDTSKGGGMIPEGQAGGCL
    CFFRARGP
    cDNA sequence of ZM65457595 from corn (SEQ ID NO: 57):
    ACCTCGCCACCCTCCTGCCTCCTCCGCATCCGCGCCCCCTCGCTTAGCCTAAACCGC
    GGGGCAGCTAGTCTCGCCACCGCAGGCCGCACCGGTCATCACACCGAAGCGCACGC
    GGGGAGCCCCCGTAGAGTTCCGGGGCGACCAGGCCAACTAACGCCATGAAGGAGGA
    AGGCGGCGGCGGGGACGCGGGGTTCGTGCGGGCGGACCAGATCGACCTCAAGAGC
    CTGGACGAGCAGCTGGAGCGCCATCTCACCCGCGCCTGGACCATGGAGAAGCGCAA
    GGAGGAGGCCTCCGCCGGCGCTGGCGCCGGCGCCAGGCAGCACCAGCAGTCCCGG
    CGCCCGCGGAGGGAGGACTGGGAGATCGACCCCGCCAAGCTTGTCGTCAAGGGCGT
    CATCGCCCGCGGCACCTTTGGCACCGTCCACCGCGGCATCTACGACGCTCACGACGT
    CGCAGTGAAACTACTTGATTGGGGAGAGGATGGGCATAGATCAGAACAAGACATTGCA
    GCACTAAGAGCAGCTTTTTCACAAGAGGTCTCTGTTTGGCATAAGCTTGACCATCCAAA
    TGTAACCAAGTTTATTGGAGCTATAATGGGTGCAAGGGATCTGAATATTCAAACGGAAA
    ACGGCCACATTGGCATGCCAACTAATATCTGCTGTGTCGTTGTGGAGTACCTTCCTGG
    TGGTGCACTAAAATCATTTCTGATAAAGAACAGGAGAAAGAAGCTAGCTTTTAAGGTCG
    TTGTTCAAATCGCTCTTGACCTTGCCAGGGGATTAAGCTATCTCCATTCCAAGAAGATT
    GTGCACCGTGATGTGAAGACTGAAAATATGCTTCTTGACAAAACGAGAACCGTGAAGA
    TCGCTGATTTTGGTGTTGCTCGCCTTGAAGCTTCAAATCCCAGTGACATGACGGGCGA
    AACTGGAACGCTTGGTTACATGACACCTGAGGTTCTCAATGGAAATCCCTACAACAGG
    AAATGCGATGTTTACAGCTTCGGGATCTGTTTGTGGGAGATATACTGCTGTGATATGCC
    ATATCCTGACTTGAGCTTTTCTGAGGTCACGTCTGCGGTTGTCCGTCAGAACCTGAGG
    CCGGAGATACCACGCTGCTGCCCGAGCTCTCTATCGAACGTGATGAAGCGCTGCTGG
    GACGCCAACCCCGACAAGCGACCTGAGATGGCCGAGGCGGTGTCCATGCTGGAGGC
    GATCGACACGTCGAAGGGTGGAGGCATGATCCCTGTGGACCAGCGGCCAGGATGCCT
    TGCGTGCTTCCGGCAGTACAGAGGTCCATGACAGATAGGTGGAAACCTGTTGGAGCT
    GCGGCCTCTAGATCTCGTGGATGCCGATCGATCGCGTGTTGTTTTCTGGGGAAGCAAA
    CTGGTTAATGGAGCTAGCCCGCCTTACCGGCTCGTGTAAATCCTCTGTCCATCAATTCT
    GTAACTCTGTTTTATCGATTAATGAAAAGAACCGGGCTTGCTCGAAAAAAAAAAAAAAA
    The ZM65457595 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 58):
    MKEEGGGGDAGFVRADQIDLKSLDEQLERHLTRAWTMEKRKEEASAGAGAGARQHQQS
    RRPRREDWEIDPAKLVVKGVIARGTFGTVHRGIYDAHDVAVKLLDWGEDGHRSEQDIAAL
    RAAFSQEVSVWHKLDHPNVTKFIGAIMGARDLNIQTENGHIGMPTNICCVVVEYLPGGALK
    SFLIKNRRKKLAFKVVVQIALDLARGLSYLHSKKIVHRDVKTENMLLDKTRTVKIADFGVARL
    EASNPSDMTGETGTLGYMTPEVLNGNPYNRKCDVYSFGICLWEIYCCDMPYPDLSFSEVT
    SAVVRQNLRPEIPRCCPSSLSNVMKRCWDANPDKRPEMAEAVSMLEAIDTSKGGGMIPV
    DQRPGCLACFRQYRGP
    cDNA sequence of ZM67230154 from corn (SEQ ID NO: 59):
    CGGCAACCCACTATCTCATGCGCTCACATGGAGACTCCCGCACGAACTGGAATCATCT
    CCGCCTCGCCACCTCTTCATCTTCTTCCCCAGTAGCCGCCGCCACCACCACTGCAGCA
    GCCAAACCACGTGACACCTCCCGCGCCGCTCAACCCCACAGCATCCGTTGCCACCGC
    CGCTCACCTCCCCGGCGCTCCCGGCTACAACCACTGC
    AAGCATGAGGCAGCCAACCAGCGCGGGCGGCGACGCTGGGTTCTTGCGCGCGGACC
    AGATCGACCTCAAGAGCCTGGACGAGCAGCTCGAGCGCCACCTCGGACATCCCGCG
    GAGCGGGTAGTTGGCCCAGTCTCTGGGACAGGGAGCCGCCGCGGCGAAACGGCCAA
    GCTGGGTCCGGAGGAGCTGACGCCACTGCAGCGGTGCCGTGAGGACTGGGAGATCG
    ACCCTACCAAGCTCATCATCAAGGGCGTCATCGCGCGCGGCACCTTTGGCACCGTCC
    ACCGCGGCGTCTACGACGGCCAGGACGTCGCTGTAAAATTGCTTGACTGGGGCGAAG
    ATGGCCATAGATCAGAACAAGAAATTGGTGCACTAAGAGCAGCGTTTGCACAAGAGGT
    CGCTGTCTGGCATAAGCTTGAGCATCCAAACGTTACTAAGTTTATTGGGGCTATAATGG
    GCGCAAGAGATTTAAATATACAAACGGAACATGGACAGCTTGGCATGCCAAGCAATAT
    TTGCTGTGTTGTTGTTGAGTACCTTGCTGGAGGTGCGCTGAAAAATTTTCTGATAAAGA
    ACAGGAGAAGGAAACTTGCCTTTAAAGTTGTGGTCCAAATAGCTCTTGACCTTGCCAG
    GGGATTATGCTACCTCCACTCAAAGAAAATAGTGCACCGTGATGTCAAGACTGAAAAC
    ATGCTTCTGGACAAGACGAGAACGGTAAAGATCGCTGATTTTGGTGTTGCTCGAGTCG
    AGGCTTCAAATCCTAGCGATATGACGGGAGAAACAGGGACGCTTGGTTACATGGCTCC
    TGAGGTTCTCAATGGCCATGCTTACAACAGGAAGTGTGACGTGTACAGCTTTGGGATC
    TGCCTGTGGGAGATATACTGCTGTGACATGCCGTACCCTGATCTCAGTTTTTCTGAGG
    TCACCTCTGCCGTCGTTCGCCAGAATCTGAGGCCTGAGATACCGCGCTGCTGCCCGA
    GCTCGCTAGCGAATGTGATGAAGCGATGCTGGGACGCGAACCCGGACAAGCGTCCCG
    AGATGGCGGAGGTGGTGTCCATGCTGGAGGCGATCGACACGTCCAAGGGTGGCGGC
    ATGATCCCTAAGGACCAGACGCAGGGCTGCCTCTCGTGCTTCCGCCAGTACCGAGGT
    CCCTAACGCAGGGTTGTTTATTTATACCCGGTGAAATGATGATATTGGTCTCTACACTA
    CAACTCAGTGTAATCTAATCGCAGAAGTGGCTATATAATGGAGAAGCTTATCATTGCTT
    GCCATGGGTGTAAATGGATGGGGCGGGGTGGTTGACGATTGGTGTGCTTGTATGCTC
    GCTTCGAGTTATAATGCTTGCTGTAAGTTAAGGTGTGGAAAAAAAAAAAAAA
    The ZM67230154 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 60):
    MRQPTSAGGDAGFLRADQIDLKSLDEQLERHLGHPAERVVGPVSGTGSRRGETAKLGPE
    ELTPLQRCREDWEIDPTKLIIKGVIARGTFGTVHRGVYDGQDVAVKLLDWGEDGHRSEQEI
    GALRAAFAQEVAVWHKLEHPNVTKFIGAIMGARDLNIQTEHGQLGMPSNICCVVVEYLAG
    GALKNFLIKNRRRKLAFKVVVQIALDLARGLCYLHSKKIVHRDVKTENMLLDKTRTVKIADFG
    VARVEASNPSDMTGETGTLGYMAPEVLNGHAYNRKCDVYSFGICLWEIYCCDMPYPDLSF
    SEVTSAVVRQNLRPEIPRCCPSSLANVMKRCWDANPDKRPEMAEVVSMLEAIDTSKGGG
    MIPKDQTQGCLSCFRQYRGP
    cDNA sequence of EST465 from moss (SEQ ID NO: 61):
    GGGCCTCCTTCCTAGCCTTCATCTGCTGCGACGATGGAGGAGCTCGCCTCATCTGATG
    TTCCGAACAAGTTGAAGAAGAAGGAATCTAAGATGAAGAAGAGGGTTATAACTCCAGG
    GGCCTTGCTGAAGGCAGTAGTAAGGTCTGGAGAGGGGACTAAACGTCCTGTAGAAGG
    TGATCAGATTATCTTCCATTATGTCACACGAACAAATCAGGGAGTGGTGGTTGAGACAT
    CGCGATCTGACTTTGGAGGAAAGGGAGTTCCTCTTAGACTTGTTCTGGGAAAAAGCAA
    AATGATTGCTGGATGGGAGGAAGGCATCACCACCATGGCCAAAGGTGAAATAGCTATG
    CTGAAAGTGCAACCTGAATTACATTATGGTGACCCGGAGTGTCCTGTACCAGTGCCCG
    AGAACTTTCCAGTTTCTGATGAGCTCCTTTACGAAGTGGAGTTGTTCAACTTCTGTAAG
    GCGAAGATTATCACAGAGGATCTTGGTGTGACAAAAGTGGTCTTAGAAGAGGGTGAGG
    GCTGGGAAACTGCAAGGCCTCCGTACGAGGTGAAGCTTTGGATTACAGGCCGGATCT
    TAGGTGGGTCCACATTTTTTACTCATAAAGAGTGCGATCCCATTCATGTTGAATTCGGC
    AAGGAACAGTTGCCAGAAGGACTTGAGAAGGCAGTCGGCACTATGACGAGGAAAGAA
    AAGTCAATTATCTACATTTCAAGTTCATACTGTACGAATTCTTCAAATGCATACAAATTG
    AATATATCTCCTCAAGCGCAAGAACTAGAATTTGAAGTGCAGTTGGTGCAGCTCATTCA
    GGTAAGAGACATGTTTGGAGATGGAGGATTGATTAAGAGACGCCTGCGAGACGGACT
    AGGTGAATTTCCTGTGGACTGTCCTCTGCAAGATAGTGTGCTTAGAGTCCACTATAAG
    GCTATGCTACCTGATGATGGCGGCAGAATATTTATTGACACCAGAAGTAATGGAGGGG
    AGCCTGTTGAGTTTGCTTCTGGTGAGGGTGTGGTACCAGAGGGACTTGAGGCAAGTTT
    GAGGTTGATGCTTCCGGGGGAGCTCGCACTGATCAACAGCGTCTCTAAGTACGCATAT
    GACAAATTTCAAAGGCCAGAGAGTGTTCCAGAGGGAGCTTCAGTCCAATGGGAAGTG
    GAATTACTGGAATTTGAGAGTGCAAAGGATTGGACGGGCCTTAATTTTCAAGAGATCAT
    GGCTGAAGCTGATTCCATAAAGACCACAGGTAACCGGTTATTTAAGGAGGGCAAGCAC
    GAGCTGGCTAAAGCTAAGTACGAAAAGGTGTTGAGGGATTTCAGACATGTAAACCCTG
    GCAGTGATGAAGAAGCAAAGGAACTACAAGACACCAATAACGCACTGCGGCTTAATGT
    AGCAGCTTGTTATCATAAACTCCATGAGTACATCAAATGCATAGAAACATGCAACAAGG
    TGCTAGAAGGTAACCCGCATCATGTCAAAGGGTTATTTCGCCGAGGAACTGCTTACAT
    GGAAACGGGGGACTTTGATGAAGCTAGAGCTGATTTCAAGCAGATGATAACAGTTGAC
    AAGGCTGTCACAGTTGATGCAACTGCTGCTTTACAGAAGCTCAAGCAAAAAGAACGGG
    AAGCTGAGCTGAAAGCTAAGAAACAGTTCAAAGGGCTATTTGACTTAAAACCTGGAGA
    ACTCTCTGAGGGGCTAGAAGAGGTAAAGCCCGTAAGCGAAATCCATGAGAAGACTGTT
    GTCAACGAGGAACTTCCGATAGCATCTATGGATCAACATCAACACTCAAAGCACGAAA
    CAGAGGAAGGGAGCCATGAATCGCCCAGGGCAAGCAGCCGATTGTTAAGACTTCTGA
    AAGGTGGAGAGCACCTGATAAGGACAGTCACTTTTGGGAAGTGTACGATTCTTTAATTT
    TTCATATTGCTACTGCTAGGATCTCCCCTTTTTACTGTACTGGTGACTACCTTATGCTCA
    TTTACATTTCTAAGCCGTTATAGCTGTTATTAACCATTCGATAATGTACTATGAACAATAT
    TCCACTAGCGTTTTATGGCTATTTTTCATTAAGTCCTCGTGCCGTTA
    The EST465 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 62):
    MEELASSDVPNKLKKKESKMKKRVITPGALLKAVVRSGEGTKRPVEGDQIIFHYVTRTNQG
    VVVETSRSDFGGKGVPLRLVLGKSKMIAGWEEGITTMAKGEIAMLKVQPELHYGDPECPV
    PVPENFPVSDELLYEVELFNFCKAKIITEDLGVTKVVLEEGEGWETARPPYEVKLWITGRIL
    GGSTFFTHKECDPIHVEFGKEQLPEGLEKAVGTMTRKEKSIIYISSSYCTNSSNAYKLNISP
    QAQELEFEVQLVQLIQVRDMFGDGGLIKRRLRDGLGEFPVDCPLQDSVLRVHYKAMLPDD
    GGRIFIDTRSNGGEPVEFASGEGVVPEGLEASLRLMLPGELALINSVSKYAYDKFQRPESV
    PEGASVQWEVELLEFESAKDWTGLNFQEIMAEADSIKTTGNRLFKEGKHELAKAKYEKVL
    RDFRHVNPGSDEEAKELQDTNNALRLNVAACYHKLHEYIKCIETCNKVLEGNPHHVKGLFR
    RGTAYMETGDFDEARADFKQMITVDKAVTVDATAALQKLKQKEREAELKAKKQFKGLFDL
    KPGELSEGLEEVKPVSEIHEKTVVNEELPIASMDQHQHSKHETEEGSHESPRASSRLLRLL
    KGGEHLIRTVTFGKCTIL
    cDNA sequence of YBL109w from yeast (SEQ ID NO: 63):
    ATGTCCCTACGGCCTTGTCTAACACCATCCAGCATGCAATACAGTGACATATATATATA
    CCCTAACACTACCCTAACCCTACCCTATTTCAACCCTTCCAACCTGTCTCTCAACTTAC
    CCTCACATTACCCTACCTCTCCACTTGTTACCCTGTCCCATTCAACCATACCACTCCCA
    ACCACCATCCATCCCTCTACTTACTACCACCAATCAACCGTCCACCATAACCGTTACCC
    TCCAATTAGCCATATTCAACTTCACTACCACTTACCCTGCCATTACTCTACCATCCACCA
    TCTGCTACTCACCATACTGTTGTTCTACCCTCCATATTAA
    The YBL109w cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 64):
    MSLRPCLTPSSMQYSDIYIYPNTTLTLPYFNPSNLSLNLPSHYPTSPLVTLSHSTIPLPTTIHP
    STYYHQSTVHHNRYPPISHIQLHYHLPCHYSTIHHLLLTILLFYPPY
    cDNA sequence of YBL100c from yeast (SEQ ID NO: 65):
    ATGTTGTTCAAACCAAAAACACGAGCAATACCATCACCGACTGCAAGAACTCTACCAGT
    TTCGTTCAAATTGGCCTCGTCGGACACACCCTTAATTCTTTCCTCTAAGATGGAGGAAA
    CTTCTGTGGGTTGTGCCTTGGTGGAAGCCAATCTTCTGGTGGAAGCCAAAGCAGCAGC
    GGCAGGTCTTGCGGCCTTGGTAGAGTTAATTAGAGTTCTCGATAGAGAACGAATAGCA
    GCAGTACGAGCCAACATTATTATATGTGCGTGTTTTTTTTATTTATTTTGTTACTGTTCTT
    GCGATAGTTATGAGAGCTAA
    The YBL100c cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 66):
    MLFKPKTRAIPSPTARTLPVSFKLASSDTPLILSSKMEETSVGCALVEANLLVEAKAAAAGL
    AALVELIRVLDRERIAAVRANIIICACFFYLFCYCSCDSYES
    cDNA sequence of YKL184w from yeast (SEQ ID NO: 67):
    ATGTCTAGTACTCAAGTAGGAAATGCTCTATCTAGTTCCACTACTACTTTAGTGGACTT
    GTCTAATTCTACGGTTACCCAAAAGAAGCAATATTATAAAGATGGCGAGACGCTGCACA
    ATCTTTTGCTTGAACTAAAGAATAACCAAGATTTGGAACTTTTACCGCATGAACAAGCG
    CATCCTAAAATATTTCAAGCGCTCAAGGCTCGTATTGGTAGAATTAATAATGAAACGTG
    CGACCCCGGTGAGGAGAACTCGTTTTTCATATGCGATTTGGGAGAAGTCAAGAGATTA
    TTCAACAACTGGGTGAAGGAGCTTCCTAGAATTAAGCCATTTTATGCCGTCAAATGTAA
    TCCTGATACCAAGGTTTTGTCATTATTAGCAGAGTTGGGCGTTAATTTCGATTGCGCTT
    CCAAAGTGGAAATTGACAGAGTATTATCGATGAACATCTCGCCGGATAGAATTGTTTAC
    GCTAATCCTTGTAAAGTAGCATCTTTCATTAGATATGCAGCTTCAAAAAATGTAATGAAG
    TCTACTTTTGACAATGTAGAAGAATTGCATAAAATCAAAAAGTTTCATCCTGAGTCTCAG
    TTGTTATTAAGAATCGCTACCGATGACTCTACCGCTCAATGTCGACTTTCCACCAAATA
    TGGCTGTGAAATGGAAAACGTAGACGTTTTATTAAAGGCTATAAAGGAACTAGGTTTAA
    ACCTGGCTGGTGTTTCTTTCCACGTCGGTTCAGGCGCTTCTGATTTTACAAGCTTATAC
    AAAGCCGTTAGAGATGCAAGAACGGTATTTGACAAAGCTGCTAACGAATACGGGTTGC
    CCCCTTTGAAGATTTTGGATGTAGGTGGTGGATTTCAATTTGAATCCTTCAAAGAATCA
    ACTGCTGTTTTGCGTCTAGCGCTAGAGGAATTTTTCCCTGTAGGTTGTGGTGTTGATAT
    AATTGCAGAGCCTGGCAGATACTTTGTAGCTACAGCGTTCACTTTGGCATCTCATGTGA
    TTGCGAAGAGAAAACTGTCTGAGAATGAAGCAATGATTTACACTAACGATGGTGTATAC
    GGGAACATGAATTGTATTTTATTCGATCATCAAGAGCCCCATCCAAGAACCCTTTATCA
    TAATTTGGAATTTCATTACGACGATTTTGAATCCACTACTGCGGTCCTCGACTCTATCAA
    CAAAACAAGATCTGAGTATCCATATAAAGTTTCCATCTGGGGACCCACATGTGATGGTT
    TGGATTGTATTGCCAAAGAGTATTACATGAAGCATGATGTTATAGTCGGTGATTGGTTT
    TATTTTCCTGCCCTGGGTGCCTACACATCATCGGCGGCTACTCAATTCAACGGCTTTGA
    GCAGACTGCGGATATAGTATACATAGACTCTGAACTCGATTAA
    The YKL184w cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 68):
    MSSTQVGNALSSSTTTLVDLSNSTVTQKKQYYKDGETLHNLLLELKNNQDLELLPHEQAHP
    KIFQALKARIGRINNETCDPGEENSFFICDLGEVKRLFNNWVKELPRIKPFYAVKCNPDTKV
    LSLLAELGVNFDCASKVEIDRVLSMNISPDRIVYANPCKVASFIRYAASKNVMKSTFDNVEE
    LHKIKKFHPESQLLLRIATDDSTAQCRLSTKYGCEMENVDVLLKAIKELGLNLAGVSFHVGS
    GASDFTSLYKAVRDARTVFDKAANEYGLPPLKILDVGGGFQFESFKESTAVLRLALEEFFP
    VGCGVDIIAEPGRYFVATAFTLASHVIAKRKLSENEAMIYTNDGVYGNMNCILFDHQEPHPR
    TLYHNLEFHYDDFESTTAVLDSINKTRSEYPYKVSIWGPTCDGLDCIAKEYYMKHDVIVGD
    WFYFPALGAYTSSAATQFNGFEQTADIVYIDSELD
    cDNA sequence of YPL091w from yeast (SEQ ID NO: 69):
    ATGCTTTCTGCAACCAAACAAACATTTAGAAGTCTACAGATAAGAACTATGTCCACGAA
    CACCAAGCATTACGATTACCTCGTCATCGGGGGTGGCTCAGGGGGTGTTGCTTCCGC
    AAGAAGAGCTGCATCTTATGGTGCGAAGACATTACTAGTTGAAGCTAAGGCTCTTGGT
    GGTACCTGTGTTAACGTGGGTTGTGTTCCGAAGAAAGTCATGTGGTATGCTTCTGACC
    TCGCTACTAGAGTATCCCATGCAAATGAATATGGATTATATCAGAATCTTCCATTAGATA
    AAGAGCATTTGACTTTTAATTGGCCAGAATTTAAGCAGAAAAGGGATGCTTATGTCCAT
    AGGTTGAACGGTATATACCAGAAGAATTTAGAAAAAGAAAAAGTGGATGTTGTATTTGG
    ATGGGCTAGATTCAATAAGGACGGTAATGTTGAAGTTCAGAAAAGGGATAATACTACTG
    AAGTTTACTCCGCTAACCATATTTTAGTTGCGACCGGTGGAAAGGCTATTTTCCCCGAA
    AACATTCCAGGTTTCGAATTAGGTACTGATTCTGATGGGTTCTTTAGATTGGAAGAACA
    ACCTAAGAAAGTTGTTGTTGTTGGCGCTGGTTATATTGGTATTGAGCTAGCAGGTGTGT
    TCCATGGGCTGGGATCCGAAACGCACTTGGTAATTAGAGGTGAAACTGTCTTGAGAAA
    ATTTGATGAATGCATCCAGAACACTATTACTGACCATTACGTAAAGGAAGGCATCAACG
    TTCATAAACTATCCAAAATTGTTAAGGTGGAGAAAAATGTAGAAACTGACAAACTGAAA
    ATACATATGAATGACTCAAAGTCCATCGATGACGTTGACGAATTAATTTGGACAATTGG
    ACGTAAATCCCATCTAGGTATGGGTTCAGAAAATGTAGGTATAAAGCTGAACTCTCATG
    ACCAAATAATTGCTGATGAATATCAGAACACCAATGTTCCAAACATTTATTCTCTAGGTG
    ACGTTGTTGGAAAAGTTGAATTGACACCTGTCGCTATTGCAGCGGGCAGAAAGCTGTC
    TAATAGACTTTTTGGTCCAGAGAAATTCCGTAATGACAAACTAGATTACGAGAACGTCC
    CCAGCGTAATTTTCTCACATCCTGAAGCCGGTTCCATTGGTATTTCTGAGAAGGAAGCC
    ATTGAAAAGTACGGTAAGGAGAATATAAAGGTCTACAATTCCAAATTTACCGCCATGTA
    CTATGCTATGTTGAGTGAGAAATCACCCACAAGATATAAAATTGTTTGTGCGGGACCAA
    ATGAAAAGGTTGTCGGTCTGCACATTGTTGGTGATTCCTCTGCAGAAATCTTGCAAGG
    GTTCGGTGTTGCTATAAAGATGGGTGCCACTAAGGCTGATTTCGATAATTGTGTTGCTA
    TTCATCCGACTAGCGCAGAAGAATTGGTTACTATGAGATAA
    The YPL091w cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 70):
    MLSATKQTFRSLQIRTMSTNTKHYDYLVIGGGSGGVASARRAASYGAKTLLVEAKALGGT
    CVNVGCVPKKVMWYASDLATRVSHANEYGLYQNLPLDKEHLTFNWPEFKQKRDAYVHRL
    NGIYQKNLEKEKVDVVFGWARFNKDGNVEVQKRDNTTEVYSANHILVATGGKAIFPENIPG
    FELGTDSDGFFRLEEQPKKVVVVGAGYIGIELAGVFHGLGSETHLVIRGETVLRKFDECIQN
    TITDHYVKEGINVHKLSKIVKVEKNVETDKLKIHMNDSKSIDDVDELIWTIGRKSHLGMGSEN
    VGIKLNSHDQIIADEYQNTNVPNIYSLGDVVGKVELTPVAIAAGRKLSNRLFGPEKFRNDKL
    DYENVPSVIFSHPEAGSIGISEKEAIEKYGKENIKVYNSKFTAMYYAMLSEKSPTRYKIVCAG
    PNEKVVGLHIVGDSSAEILQGFGVAIKMGATKADFDNCVAIHPTSAEELVTMR
    cDNA sequence of TA54587433 from wheat (SEQ ID NO: 71):
    ATGGCGGTCATGTCACGGTTGAAGAGGCTGGCGGCGCCCGCGCTGCTGGTGCTGCTT
    GCGCTGGCGGCGTCCGCGGCCGTGGCGGCGAAGACGACCCAGGACGGCGCGGAGG
    CGGCGCCGGGCAAGGATGAAGAGTCGTGGACGGGGTGGGCCAAGGACAAGATCTCC
    GAGGGGCTGGGGCTCAAGCACGACGCTGACGAGGAGGCCGCGCGCGAGACCGTCC
    AGCACACCGCCTCCGAGACGGGGAGTCAGGTGAGCGGCAAGGCAGCGGACGCCAAG
    GAGGCGGCCAAGGGAACGGTCGGGGAGAAGCTCGGGGAGGTGAAGGACAAGGTCA
    CCGGCGCAGCAGCCGACGGCAAGGACAAGACGCACCGCAAGGATGACTTGCTGTGA
    The TA54587433 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 72):
    MAVMSRLKRLAAPALLVLLALAASAAVAAKTTQDGAEAAPGKDEESWTGWAKDKISEGLG
    LKHDADEEAARETVQHTASETGSQVSGKAADAKEAAKGTVGEKLGEVKDKVTGAAADGK
    DKTHRKDDLL
    cDNA sequence of ZM68532504 from corn (SEQ ID NO: 73):
    ATGCCGTCGCACGGGGATCTGGACCGGCAGATCGCGCAGCTGCGCGACTGCAAGTA
    CCTGCCCGAGGCGGAGGTCAAGGCGCTCTGCGAGCAGGCCAAGGCCATCCTTATGG
    AGGAGTGGAACGTGCAGCCCGTGCGCTGTCCTGTCACCGTCTGTGGCGACATCCACG
    GCCAGTTCTATGACCTCATCGAGCTCTTCCGCATCGGCGGCGACGCTCCCGACACCA
    ACTACCTCTTCATGGGCGACTACGTCGATCGTGGGTACTATTCAGTTGAAACAGTTTCT
    CTGTTAGTGGCTTTGAAAGTCCGTTACAGAGATAGAATTACAATACTTAGAGGAAATCA
    TGAGAGCAGACAAATCACTCAAGTATATGGCTTCTATGATGAATGCTTAAGAAAGTATG
    GAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTC
    TTATAGAAAATCAGGTCTTCTGTCTTCACGGTGGCCTCTCTCCGTCATTGGACACGTTG
    GATAATATTCGTTCTCTTGATCGCGTACAGGAGGTTCCTCATGAAGGACCCATGTGTGA
    TCTTTTGTGGTCTGACCCAGATGACCGATGTGGATGGGGAATTTCACCAAGAGGAGCA
    GGTTACACATTTGGGCAAGACATTGCGCAGCAGTTCAACCATACAAATGGTCTTTCTCT
    CATTTCAAGGGCCCATCAACTTGTAATGGAAGGATTTAATTGGTGCCAGGATAAGAATG
    TAGTCACAGTCTTCAGCGCGCCTAATTATTGTTACCGCTGTGGTAACATGGCTGCTATT
    CTTGAAATCGGGGAAAACATGGACCAGAACTTCCTTCAATTCGACCCGGCACCTCGGC
    AAATTGAGCCAGACACAACTCGGAAAACCCCAGACTACTTTTTGTAA
    The ZM68532504 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 74):
    MPSHGDLDRQIAQLRDCKYLPEAEVKALCEQAKAILMEEWNVQPVRCPVTVCGDIHGQFY
    DLIELFRIGGDAPDTNYLFMGDYVDRGYYSVETVSLLVALKVRYRDRITILRGNHESRQITQ
    VYGFYDECLRKYGNANVWKYFTDLFDFLPLTALIENQVFCLHGGLSPSLDTLDNIRSLDRV
    QEVPHEGPMCDLLWSDPDDRCGWGISPRGAGYTFGQDIAQQFNHTNGLSLISRAHQLVM
    EGFNWCQDKNVVTVFSAPNYCYRCGNMAAILEIGENMDQNFLQFDPAPRQIEPDTTRKTP
    DYFL
    cDNA sequence of BN42856089 from canola (SEQ ID NO: 75):
    AAAACTCCAAAAACAAACCATTTTCCATCTCTCAGGCCAAAAAAACCAGAGATTTGATC
    TCTCTGGCGATTCATCATCCTCTTCATCCACCACACGCCGTATAAGTTAAAGGATCGGT
    GGTGGTCTCTCGATGCCGCCGAACGGAGATCTAGACCGTCAGATCTCCCAGCTGATG
    GAGTGTAAACCGCTATCTGAGGCCGATGTGAAGACGCTCTGCGATCAAGCGAGGGCC
    ATCCTCGTCGAGGAGTGGAACGTTCAGCCCGTGAAGTGTCCTGTCACCGTCTGCGGC
    GATATCCACGGACAGTTCTATGACCTTATCGAGCTCTTTCGAATCGGTGGGAATCCTC
    CGGATACTAACTACCTCTTCATGGGAGACTATGTAGACCGTGGCTACTATTCAGTAGAA
    ACAGTTTCTCTATTGGTGGCACTGAAAGTGCGATACAGGGATAGGATTACAATCTTGC
    GAGGGAATCACGAGAGTCGGCAGATTACTCAAGTCTATGGGTTTTATGATGAATGTTT
    GAGGAAGTATGGAAATGCAAATGTCTGGAAGTTTTTCACGGACCTTTTCGATTATCTTC
    CTCTTACTGCTCTCATAGAGAGTCAGGTTTTCTGCTTGCATGGAGGGCTTTCACCTTCT
    CTGGACACCCTTGATAATATCCGAAGCTTGGATCGTATACAAGAGGTTCCACATGAAG
    GACCAATGTGTGATTTATTATGGTCTGATCCCGATGATCGATGTGGGTGGGGAATATCT
    CCACGAGGTGCTGGTTATACATTTGGACAAGACATCGCAACTCAGTTTAATCACAACAA
    TGGACTCAGTCTCATATCAAGAGCACATCAACTTGTCATGGAAGGCTTTAACTGGTGTC
    AGGACAAAAATGTTGTGACGGTGTTTAGTGCACCAAACTATTGCTACCGGTGTGGAAA
    CATGGCAGCTATTCTAGAGATAGGAGAGAACATGGACCAGAACTTCCTCCAGTTCGAT
    CCAGCTCCTCGTCAAGTCGAACCAGATACTACCCGCAAGACCCCTGATTATTTTTTGTG
    ATTTATTTGCATTTTTTTTTCTTTTGTTCCCAACCATTTATAATTTTTAAAAAATCTGTTTT
    ATCTTGCTTATGAATAATCATTCTAGTGTCTCTTCAAAAAAAAAAAAAAA
    The BN42856089 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 76):
    MPPNGDLDRQISQLMECKPLSEADVKTLCDQARAILVEEWNVQPVKCPVTVCGDIHGQFY
    DLIELFRIGGNPPDTNYLFMGDYVDRGYYSVETVSLLVALKVRYRDRITILRGNHESRQITQ
    VYGFYDECLRKYGNANVWKFFTDLFDYLPLTALIESQVFCLHGGLSPSLDTLDNIRSLDRIQ
    EVPHEGPMCDLLWSDPDDRCGWGISPRGAGYTFGQDIATQFNHNNGLSLISRAHQLVME
    GFNWCQDKNVVTVFSAPNYCYRCGNMAAILEIGENMDQNFLQFDPAPRQVEPDTTRKT-
    PDYFL
    cDNA sequence of BN43206527 from canola (SEQ ID NO: 77):
    CCAAAGACCATTTGATCTCTGGCGATTTCATCTTCCGATATGCCGCCGAACGGAGATC
    TAGACCGTCAGATCGAGCATCTGATGGAGTGCAAACCTTTATCGGAGGAGGATGTGAG
    GACGCTCTGCGATCAAGCTAAGGCCATCCTCGTCGAGGAATGGAACGTCCAGCCCGT
    GAAATGCCCCGTCACCGTCTGCGGCGATATCCACGGCCAGTTCTATGACCTTATCGAG
    CTTTTCCGAATCGGTGGTAACGCCCCCGATACGAATTACCTCTTCATGGGTGACTATGT
    AGACCGTGGCTACTATTCAGTGGAAACGGTTTCTTTATTGGTGGCATTGAAAGTCAGAT
    ACAGGGATAGGATTACAATCTTGCGAGGGAACCACGAGAGTCGTCAGATCACCCAAGT
    ATATGGTTTTTATGACGAGTGCTTGAGGAAGTACGGAAACGCAAATGTGTGGAAGTATT
    TCACAGACCTTTTCGATTATCTTCCTCTTACTGCTCTTATCGAGAGTCAGGTTTTCTGTT
    TGCATGGAGGGCTATCACCTTCTCTGGATACACTTGATAATATCCGAAGCTTGGATCGT
    ATACAAGAGGTTCCACACGAAGGACCAATGTGTGATTTACTATGGTCTGATCCAGATGA
    TCGATGCGGGTGGGGAATATCTCCAAGAGGTGCTGGTTATACATTTGGACAGGATATA
    GCAACTCAGTTTAATCACAACAATGGACTCAGTCTCATATCAAGAGCGCATCAGCTTGT
    CATGGAAGGTTTTAACTGGTGTCAGGATAAGAATGTGGTGACGGTGTTTAGTGCACCA
    AACTATTGCTACCGGTGTGGAAACATGGCAGCGATTCTAGAGATAAGTGAGAACATGG
    AGCAGAACTTCCTTCAGTTTGATCCAGCTCCAAGACAAGTCGAACCTGATACTACCCGT
    AAGACCCCTGATTATTTTTTGTGATTTTATTTGTATTTTTTTTTCTTCTAAGCGGAGTTCG
    AGTTTCCCTCAAAACGAAAGAAAGAAACAAACATCATTTTGTTGTTGTTGATGTGATTGC
    TGAGAACAAAGTTTGTAGTAGAAGCGTCTATATATAGAATAGTGTCTTCTCATTGTCATT
    TCACTTGTTACTGCATAGAGGAATGAGGTTTCGAACCCTGCCCGCCACTTTCATTTCAG
    TTTCATTTATAAAATATGAGTTTGATACCGAAAAAAAAAAAAAAA
    The BN43206527 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 78):
    MPPNGDLDRQIEHLMECKPLSEEDVRTLCDQAKAILVEEWNVQPVKCPVTVCGDIHGQFY
    DLIELFRIGGNAPDTNYLFMGDYVDRGYYSVETVSLLVALKVRYRDRITILRGNHESRQITQ
    VYGFYDECLRKYGNANVWKYFTDLFDYLPLTALIESQVFCLHGGLSPSLDTLDNIRSLDRIQ
    EVPHEGPMCDLLWSDPDDRCGWGISPRGAGYTFGQDIATQFNHNNGLSLISRAHQLVME
    GFNWCQDKNVVTVFSAPNYCYRCGNMAAILEISENMEQNFLQFDPAPRQVEPDTTRKT-
    PDYFL
    cDNA sequence of HA66872964 from sunflower (SEQ ID NO: 79):
    CTAAAAATATCTTTAACCGCCGGCTGCCATGACGGAACCCTAAGCAACTTCTCCGGCG
    ACTCCGGCGGAGCTCCGTTCAACCTAAATGCGAATCATTCTTCCAGATCTTCAAATCCG
    AACACACAAATCACGTAACAATGCCGTCGCAATCGGATCTGGACCGTCAGATCGAGCA
    CTTGATGGACTGTAAACCGCTGCCGGAGGCGGAGGTGCGGACGTTGTGTGATCAGGC
    GAGGACGATTTTGGTCGAGGAGTGGAATGTGCAGCCGGTGAAGTGTCCGGTGACTGT
    TTGCGGTGATATTCATGGGCAGTTTCATGATTTGCTTGAGCTGTTTCGGATCGGAGGA
    AGTGCTCCGGACACGAATTACTTGTTTATGGGAGATTATGTTGATCGAGGCTATTACTC
    GGTGGAGACTGTTACGCTTCTTGTGGCATTGAAAGTTCGTTACAGAGATAGGATTACTA
    TTCTCAGAGGAAACCATGAGAGCAGGCAGATAACTCAAGTGTATGGATTTTACGATGA
    ATGCTTGAGGAAGTACGGAAACGCAAATGTATGGAAACATTTCACTGACCTTTTTGATT
    ATCTACCTCTCACTGCCCTTATCGAGAGTCAGATATTCTGTCTCCATGGTGGCTTGTCT
    CCATCTTTGGATACACTAGATAACATACGTGCTTTAGATCGCATACAAGAGGTTCCTCA
    TGAGGGGCCAATGTGTGACCTTTTGTGGTCTGATCCTGATGACCGGTGTGGTTGGGG
    AATATCTCCTCGTGGAGCCGGTTACACTTTCGGGCAGGATATAGCCGCACAGTTTAAC
    CATACAAACGGGCTCTCGCTTATTTCTCGGGCTCACCAGCTTGTCATGGAAGGTTACA
    ATTGGTCTCAGGAGAACAACGTTGTAACCATATTTAGTGCACCAAACTACTGCTATAGA
    TGCGGGAATATGGCTGCGATACTTGAGGTTGGAGAGAATATGGACCAGAATTTCTTAC
    AATTTGACCCAGCCCCTCGTCAGGTTGAGCCCGATGTTGCACGAAGAACTCCGGATTA
    CTTCCTGTAAATTTGTGTTGGATAATATGACCTTTGCATGCATCCTATTTATGTTGTTAT
    AGTTTTCGCTTTCCCCTGCTAGAGAGTCCCCCTATTCTTGAGAATTAAAGACAATATGT
    ATGATTGTTTGTCCCTTGTTCTATTTGAGATTATTTGTTTAAAAAAAAAAAAAAA
    The HA66872964 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 80):
    MPSQSDLDRQIEHLMDCKPLPEAEVRTLCDQARTILVEEWNVQPVKCPVTVCGDIHGQFH
    DLLELFRIGGSAPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYRDRITILRGNHESRQITQ
    VYGFYDECLRKYGNANVWKHFTDLFDYLPLTALIESQIFCLHGGLSPSLDTLDNIRALDRIQ
    EVPHEGPMCDLLWSDPDDRCGWGISPRGAGYTFGQDIAAQFNHTNGLSLISRAHQLVME
    GYNWSQENNVVTIFSAPNYCYRCGNMAAILEVGENMDQNFLQFDPAPRQVEPDVARRT-
    PDYFL
    cDNA sequence of LU61662612 from linseed (SEQ ID NO: 81):
    CATCTCTCTTTCTCTCTCTTCCATTTTCGTTCTTTTGAATCTCCGTTAGCCCTACAAATC
    CATGGTCATGGCCTGAGAGAGATAGAGGGATAGAGCTCTCAGTTCCTAATCACCTTAC
    CTGACCTAACCCCACGGACATATTATCGAAGGTCTGCGAGCAGGAGAGCGCAGGAGG
    AAGAGTGGGGCCAGGGTACGATGCCGTCCCACGCCGATCTGGACCGTCAGATCGAG
    CACTTGATGCAGTGCAAGCCACTTTCTGAGGCCGAAGTGAAGGCTCTCTGCGAGCAG
    GCCAGGGCCGTCCTCGTCGAGGAATGGAACGTCCAGCCGGTCAAGTGTCCGGTGACT
    GTCTGCGGCGACATCCACGGCCAGTTTCACGATCTTGTCGAGCTCTTTCGAATCGGAG
    GAAACGCCCCTGACACGAACTACCTCTTCATGGGCGACTATGTAGATCGAGGGTATTA
    TTCGGTGGAGACTGTCACCCTTCTAGTCGCCTTGAAAGTAAGATATAGAGATAGGATC
    ACAATTCTGAGAGGAAATCATGAAAGTCGTCAAATAACTCAAGTGTATGGATTCTATGA
    TGAGTGCTTGAGAAAATATGGAAATGCCAATGTGTGGAAACATTTTACCGATCTCTTTG
    ATTATCTACCACTTACAGCTCTGATTGAGAGTCAGGTCTTCTGCTTACATGGTGGACTT
    TCCCCTTCACTAGACACGCTAGACAACATTCGCTCCCTTGATCGTATCCAAGAGGTTCC
    GCACGAGGGTCCTATGTGCGACCTCCTATGGTCGGACCCGGATGACCGTTGCGGGTG
    GGGGATCTCTCCTCGTGGAGCTGGCTACACCTTTGGACAGGACATATCTCAACAGTTC
    AACCACACGAACGGCCTTTCTCTCATATCCAGAGCTCACCAGCTGGTCATGGAAGGTT
    ACAATTGGGCCCAGGACAAGAATGTGGTGACGGTGTTCAGCGCCCCGAACTACTGCT
    ACCGGTGTGGGAACATGGCGGCCATTCTCGAGATCGGAGAGAACATGGAGCAGAACT
    TCCTGCAGTTCGACCCAGCTCCTCGACAGATCGAACCGGAGACGACTCGCAGAACAC
    CCGATTATTTTTTGTGAAATGCATAGCTTCTTCTTCCTCCCTCCTTCTTGCTTGGAAATG
    GGATCCGTGTCCATTTTTTCTAATCGCCTGCCCTGCTATGTGCTTATGTTTTTTGTAGAT
    GCATTCATCATCATCATATCCAGAATAGAGAAGAAATTTTGGTGTTTGCTTTGATTGAGA
    AAAGGCGGGGAGGGAAAAATCGGCCTCTAGAGATGCTGGGTGTTGTCATTTTTCTTCT
    TCTTCTTCCTCCTTTTGGGATGGTTTCGTTTTTACTTTTTCTTTTGGGTTTCTATTGTTTA
    TCCTGCATTCATTTGAGTTTAACAAAGTTTATTATTTACAGTCTGGGTGTGTTATTAATAT
    TATTCACTGTGGTCTTGTACCAAAAAAAAAAAAAAA
    The LU61662612 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 82):
    MPSHADLDRQIEHLMQCKPLSEAEVKALCEQARAVLVEEWNVQPVKCPVTVCGDIHGQF
    HDLVELFRIGGNAPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYRDRITILRGNHESRQIT
    QVYGFYDECLRKYGNANVWKHFTDLFDYLPLTALIESQVFCLHGGLSPSLDTLDNIRSLDRI
    QEVPHEGPMCDLLWSDPDDRCGWGISPRGAGYTFGQDISQQFNHTNGLSLISRAHQLVM
    EGYNWAQDKNVVTVFSAPNYCYRCGNMAAILEIGENMEQNFLQFDPAPRQIEPETTRRTP
    DYFL
    cDNA sequence of OS32806943 from rice (SEQ ID NO: 83):
    GAGGCTTGAGCTCCACCTCCACCTCCTCCACCTCCAACCCCCGATCCCCCGCAAACC
    CTAGCCCTCTCCCCCACCCTCCTCGCCGGCGGCGAGCGGCGGCGGCGCGCGGCGG
    GACCCGGAGCCCCCAGTAGGGCCTCCTCCGTCCTCCCCTCCCTGAGGTGCGGGGGA
    GAGGATGCCGTCGTCGCACGGGGATCTGGACCGGCAGATCGCGCAGCTGCGGGAGT
    GCAAGCACCTGGCGGAGGGGGAGGTGAGGGCGCTGTGCGAGCAGGCGAAGGCCAT
    CCTCATGGAGGAGTGGAACGTGCAGCCGGTGCGGTGCCCCGTCACGGTCTGCGGCG
    ACATCCACGGCCAGTTCTACGACCTCATCGAGCTCTTCCGCATCGGCGGCGAGGCGC
    CCGACACCAACTACCTCTTCATGGGCGACTACGTCGACCGTGGCTACTACTCAGTGGA
    GACTGTTTCGTTGTTGGTGGCTTTGAAAGTACGCTACAGAGATCGAATTACAATATTGA
    GAGGAAATCATGAGAGCAGACAAATCACTCAAGTGTACGGCTTCTACGATGAATGCTT
    GAGAAAGTATGGAAATGCAAATGTATGGAAATACTTTACAGACTTGTTTGATTATTTGCC
    TCTCACAGCTCTTATAGAAAACCAGGTGTTCTGCCTTCACGGTGGTCTCTCTCCATCAT
    TGGATACTTTAGATAACATCCGTGCTCTTGATCGTATACAAGAGGTTCCTCATGAAGGA
    CCCATGTGTGATCTTTTGTGGTCTGACCCAGATGACAGATGCGGGTGGGGAATTTCAC
    CGAGAGGAGCAGGTTATACATTTGGGCAAGATATCGCTCAACAGTTTAACCATACAAAT
    GGTCTATCTCTCATCTCAAGGGCACATCAACTTGTAATGGAAGGATTTAATTGGTGTCA
    GGACAAGAATGTTGTGACGGTCTTCAGTGCACCAAACTACTGTTATCGCTGTGGTAAC
    ATGGCTGCAATTCTTGAGATTGGCGAAAACATGGATCAGAACTTCCTCCAATTTGATCC
    AGCTCCTCGGCAAATTGAACCAGACACAACACGCAAGACTCCCGACTACTTTTTGTAAT
    TTGTGGTGTTGACAATTTTAACTCACCTGTGTTGATGCTCCTCTCCTCCGCGGTGTCGG
    GGTCTGTAGATCTTCTGTCCTTAGATACGGGTTCCACGAGCCCGGCTGTATGTCTCTC
    AATTCTTTTGTTTGGAGATTTTGTTGCTGCTTCTCAACCTTTATACAAGACGTTAAAAGT
    TACATGCACTGGATTTTTTTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAA
    AAAAAAAAAAA
    The OS32806943 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 84):
    MPSSHGDLDRQIAQLRECKHLAEGEVRALCEQAKAILMEEWNVQPVRCPVTVCGDIHGQF
    YDLIELFRIGGEAPDTNYLFMGDYVDRGYYSVETVSLLVALKVRYRDRITILRGNHESRQIT
    QVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALIENQVFCLHGGLSPSLDTLDNIRALDRI
    QEVPHEGPMCDLLWSDPDDRCGWGISPRGAGYTFGQDIAQQFNHTNGLSLISRAHQLVM
    EGFNWCQDKNVVTVFSARNYCYRCGNMAAILEIGENMDQNFLQFDPAPRQIEPDTTRKTP
    DYFL
    cDNA sequence of OS34738749 from rice (SEQ ID NO: 85):
    GGTCGACGCCGTCACCGTCGCGCCAACTGCCGCAAACCGAATAAACCGAATCGATCT
    GAGAGAAGAAGAAGAAGAAGACGCGATCTCGGAGGTGGGAGCGAAACGAAACGATG
    CCGTCTCACGCGGATCTGGAACGACAGATCGAGCAGCTGATGGAGTGCAAGCCTCTG
    TCGGAGTCGGAGGTGAAGGCGCTGTGTGATCAAGCGAGGGCGATTCTCGTGGAGGAA
    TGGAACGTGCAACCGGTGAAGTGCCCCGTCACCGTCTGCGGCGATATTCACGGCCAG
    TTTTACGATCTCATCGAGCTGTTTCGGATTGGAGGGAACGCACCCGATACCAATTATCT
    CTTCATGGGTGATTATGTAGATCGTGGATACTATTCAGTGGAGACTGTTACACTTTTGG
    TGGCTTTGAAAGTCCGTTACAGAGATAGAATCACAATTCTCAGGGGAAATCATGAAAGT
    CGTCAAATTACTCAAGTGTATGGCTTCTATGATGAATGCTTGAGAAAATATGGAAATGC
    CAATGTCTGGAAATACTTTACAGACTTGTTTGATTATTTACCTCTGACTGCCCTCATTGA
    GAGTCAGATTTTCTGCTTGCATGGAGGTCTCTCACCTTCTTTGGATACACTGGATAACA
    TCAGAGCATTGGATCGTATACAAGAGGTTCCACATGAAGGACCAATGTGTGATCTCTT
    GTGGTCTGACCCTGATGATCGCTGTGGATGGGGAATATCTCCACGTGGTGCAGGATA
    CACATTTGGACAGGATATAGCTGCTCAGTTTAATCATACCAATGGTCTCTCCCTGATAT
    CGAGAGCTCATCAGCTTGTTATGGAAGGATTCAATTGGTGCCAGGACAAAAATGTGGT
    GACTGTATTTAGTGCACCAAATTACTGTTACCGATGTGGGAATATGGCTGCTATACTAG
    AAATAGGAGAGAATATGGATCAGAATTTCCTTCAGTTTGATCCAGCGCCCAGGCAAATT
    GAGCCTGACACCACACGCAAGACTCCAGATTATTTTTTATAATTTCATTTATCTGCCTGT
    TTGTAGTTACTGCTCTCTGCCATTACTGTAGATGTGTCTTTAAGGAAAGGAGTTTTGCT
    GTTTAAGTGGAGGGTGGTCATCAACATAATTCTTTCTTTTGGAGTTTACCTCCTGCTGC
    TGCCGCTGCCGCTGCCTTATTTGTACAAGAAACCAATAGAACTGACACAAGCCACCAA
    TTGGGGTTGTATATTTTTGGGAGGAAGCGGTAATAACATGGTATATCTTGTTCTGTAAT
    CCTTTTTCTTTAAATTGAATCTCAAGTTAGAGAGCAAAAAAAAAAAAA
    The OS34738749 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 86):
    MPSHADLERQIEQLMECKPLSESEVKALCDQARAILVEEWNVQPVKCPVTVCGDIHGQFY
    DLIELFRIGGNAPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYRDRITILRGNHESRQITQ
    VYGFYDECLRKYGNANVWKYFTDLEDYLPLTALIESQIFCLHGGLSPSLDTLDNIRALDRIQ
    EVPHEGPMCDLLWSDPDDRCGWGISPRGAGYTFGQDIAAQFNHTNGLSLISRAHQLVME
    GFNWCQDKNVVTVFSAPNYCYRCGNMAAILEIGENMDQNFLQFDPAPRQIEHDTTRKTP-
    DYFL
    cDNA sequence of ZM59400933 from corn (SEQ ID NO: 87):
    CTGACCGCCAGCGGGCCCGCAGGCCGGAGAAGGAGTCGGAGTCGCCCCCACCCACC
    CACCCTCTGCCGCGGGCGGGGAGCGGGCGGCGGACGAGATGCCGTCGCACGGGGA
    TCTGGACCGGCAGATCGCGCAGCTGCGCGACTGCAAGTACCTGCCCGAGGCGGAGG
    TCAAGGTGCTCTGCGAGCAGGCCAAGGCCATCCTCATGGAGGAATGGAACGTGCAGC
    CCGTGCGCTGCCCCGTCACCGTCTGCGGCGACATCCACGGCCAGTTCTATGACCTCA
    TCGAGCTCTTCCGCATCGGCGGCGACTCTCCCGACACCAACTACCTCTTCATGGGCG
    ACTACGTCGATCGTGGCTATTATTCAGTTGAAACGGTTTCTCTGTTAGTGGCTTTGAAA
    GTCCGTTACAGAGATAGAATTACAATACTTCGAGGAAATCATGAGAGCAGACAAATCAC
    TCAAGTGTACGGCTTCTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTATGGA
    AGTATTTTACAGACTTGTTTGATTATTTGCCTCTCACAGCTCTTATAGAAAATCAGGTCT
    TCTGTCTTCATGGAGGCCTCTCTCCGTCATTGGACACATTGGATAACATTCGTTCTCTT
    GATCGCATACAGGAGGTACCTCATGAAGGACCCATGTGTGATCTTTTGTGGTCTGACC
    CAGATGACCGATGTGGGTGGGGAATTTCACCCAGAGGAGCAGGTTACACATTTGGGC
    AAGACATTGCACAGCAGTTCAACCATACAAATGGTCTCTCTCTCATTTCAAGGGCCCAT
    CAACTTGTAATGGAAGGATTTAATTGGTGCCAGGATAAGAATGTAGTCACAGTCTTCAG
    TGCGCCTAATTACTGTTACCGCTGTGGTAACATGGCTGCTATTCTTGAAATCGGGGAAA
    ACATGGACCAGAACTTCCTTCAATTCAACCCCGCACCTCGGCAAATTGAGCCAGACAC
    AACTCGCAAAACCCCAGACTACTTTCTGTAATTGTGGTGGTGACCTTAACTTTCTGGTG
    TTTGATGCTCCTCTCTTCCGCAGCATCAGGGTATGTAGATCTTGTCCTTAGATATGGGT
    CCCATGTGCCCGGCCTTAACGTCTCCCTATTCTTTTGTTTGGAGATTTTGTTTCTGCTT
    CTCGATCTTGATACAAGATGTTAGAAGTTGAATGCCAGTGTATTTTTTT-
    CAAAAAAAAAAA
    The ZM59400933 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 88):
    MPSHGDLDRQIAQLRDCKYLPEAEVKVLCEQAKAILMEEWNVQPVRCPVTVCGDIHGQFY
    DLIELFRIGGDSPDTNYLFMGDYVDRGYYSVETVSLLVALKVRYRDRITILRGNHESRQITQ
    VYGFYDECLRKYGNANVWKYFTDLFDYLPLTALIENQVFCLHGGLSPSLDTLDNIRSLDRIQ
    EVPHEGPMCDLLWSDPDDRCGWGISPRGAGYTFGQDIAQQFNHTNGLSLISRAHQLVME
    GFNWCQDKNVVTVFSAPNYCYRCGNMAAILEIGENMDQNFLQFNPAPRQIEPDTTRKTP-
    DYFL
    cDNA sequence of ZM62132060 from corn (SEQ ID NO: 89):
    AATCGTCGCTCCACCTCCTCCTCGTCTATCGCCGATCTCCCCCAAACCCTAGCCCCGA
    CCTGACCGCCGGCGGGCCCGCCGGCCGGAGAAGGAGTCGCTCCCACCCATCCAACT
    TCTGCGGCGGAAGGGGAGCGGGCGGCGGACGAGATGCCGTCGCACGGGGATCTGG
    ACCGGCAGATCGCGCAGCTGCGCGACTGCAAGTACCTGCCCGAGGCGGAGGTCAAG
    GCGCTCTGCGAGCAGGCCAAGGCCATCCTTATGGAGGAGTGGAACGTGCAGCCCGT
    GCGCTGTCCTGTCACCGTCTGTGGCGACATCCACGGCCAGTTCTATGACCTCATCGAG
    CTCTTCCGCATCGGCGGCGACGCTCCCGACACCAACTACCTCTTCATGGGCGACTAC
    GTCGATCGTGGGTACTATTCAGTTGAAACAGTTTCTCTGTTAGTGGCTTTGAAAGTCCG
    TTACAGAGATAGAATTACAATACTTAGAGGAAATCATGAGAGCAGACAAATCACTCAAG
    TATATGGCTTCTATGATGAATGCTTAAGAAAGTATGGAAATGCAAATGTCTGGAAGTAT
    TTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGGTCTTCTGT
    CTTCACGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAATATTCGTTCTCTTGATCG
    CGTACAGGAGGTTCCTCATGAAGGACCCATGTGTGATCTTTTGTGGTCTGACCCAGAT
    GACCGATGTGGATGGGGAATTTCACCAAGAGGAGCAGGTTACACATTTGGGCAAGAC
    ATTGCGCAGCAGTTCAACCATACAAATGGTCTTTCTCTCATTTCAAGGGCCCATCAACT
    TGTAATGGAAGGATTTAATTGGTGCCAGGATAAGAATGTAGTCACAGTCTTCAGCGCG
    CCTAATTATTGTTACCGCTGTGGTAACATGGCTGCTATTCTTGAAATCGGGAAAAACAT
    GGACCAGAACTTCCTTCAATTCGACCCGGCACCTCGGCAAATTGAGCCAGACACAACT
    CGGAAAACCCCAGACTACTTTTTGTAATTGTGGTGGTGACATTAACTTACTGGTGTTGA
    TGCTCCTCTTTTCCGCAGCATCAGGGTCTGTAGATCATCTGTCCTTAGATATGGGTTCC
    ATGAGCCCGACCTGTACGTCTCCCAATTCTTTTGTTTGGAGATTTTGTTGCCGCTTAAC
    GATCTTTATACAATATGTTAAAAAGTTAAATGCCATTGGATTTTTCTCCAAAAAAAAAAA
    The ZM62132060 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 90):
    MPSHGDLDRQIAQLRDCKYLPEAEVKALCEQAKAILMEEWNVQPVRCPVTVCGDIHGQFY
    DLIELFRIGGDAPDTNYLFMGDYVDRGYYSVETVSLLVALKVRYRDRITILRGNHESRQITQ
    VYGFYDECLRKYGNANVWKYFTDLFDFLPLTALIENQVFCLHGGLSPSLDTLDNIRSLDRV
    QEVPHEGPMCDLLWSDPDDRCGWGISPRGAGYTFGQDIAQQFNHTNGLSLISRAHQLVM
    EGFNWCQDKNVVTVFSAPNYCYRCGNMAAILEIGKNMDQNFLQFDPAPRQIEPDTTRKTP
    DYFL
    cDNA sequence of ZM59202533 from corn (SEQ ID NO: 91):
    ATGAAGGGGAAGAAGCCGGTCAAGGAGCTCAAGCTCACCGTGCCGGCGCAGGAGAC
    CCCGGTAGACAAGTTCCTGACGGCAAGTGGCACGTTCAAGGATGGTGAGCTGAGGCT
    CAATCAGAGCGGCTTGCGGCTTATCTCTGAGGAAAACGGGGATGAAGATGAATCTACA
    AAGCTGAAGGTGGAAGATGTGCAGTTATCAATGGATGATCTTGAGATGATTCAAGTCAT
    TGGCAAAGGAAGCGGTGGTGTTGTCCAGCTAGTGAGGCACAAATGGGTGGGCACATT
    GTTTGCCTTAAAGGGTATTCAAATGAACATTCAGGAGTCAGTTCGTAAACAAATAGTAC
    AGGAGCTCAAAATAAACCAAGCAACACAGAGCCCTCATATAGTTATGTGCCATCAATCT
    TTTTACCACAATGGTGTAATATATCTTGTTCTTGAGTACATGGACCGTGGATCGCTTGC
    AGACATTGTTAAGCAAGTGAAGACTATTCTGGAGCCATACCTTGCAGTACTTTGTAAGC
    AGGTCTTGGAGGGTTTATTGTATCTTCATCATCAAAGGCACGTGATTCACAGGGACATA
    AAACCATCTAACTTGTTGGTCAACCGTAAAGGTGAAGTCAAGATTACCGACTTCGGAGT
    GAGTGCTGTGCTAGCAAGCTCAATAGGTCAGCGAGATACATTTGTTGGAACCTACAAC
    TATATGGCGCCTGAGCGGATTAGTGGTAGCACTTATGACTACAAAAGTGACATATGGA
    GTTTGGGCTTAGTTATACTTGAGTGTGCCATTGGCCGGTTCCCTTATATACCTTCGGAA
    GGTGAAGGTTGGTTAAGCTTTTATGAACTTCTGGAGGCCATTGTCGATCAGCCACCAC
    CTTCTGCACCTGCAGATCAGTTCTCTCCAGAATTCTGCTCATTTATCTCCTCTTGCATAC
    AGAAAGATCCGGCTCAGAGGATGTCTGCTTCAGAACTCTTGAATCACCCTTTTTTGAAG
    AAGTTCGAGGATAAGGACTTAAACCTGGGGATTCTTGTGGAGAACCTGGAACCTCCAA
    TGAATATACCCGAATAG
    The ZM59202533 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 92):
    MKGKKPVKELKLTVPAQETPVDKFLTASGTFKDGELRLNQSGLRLISEENGDEDESTKLKV
    EDVQLSMDDLEMIQVIGKGSGGVVQLVRHKWVGTLFALKGIQMNIQESVRKQIVQELKINQ
    ATQSPHIVMCHQSFYHNGVIYLVLEYMDRGSLADIVKQVKTILEPYLAVLCKQVLEGLLYLH
    HQRHVIHRDIKPSNLLVNRKGEVKITDFGVSAVLASSIGQRDTFVGTYNYMAPERISGSTYD
    YKSDIWSLGLVILECAIGRFPYIPSEGEGWLSFYELLEAIVDQPPPSAPADQFSPEFCSFISS
    CIQKDPAQRMSASELLNHPFLKKFEDKDLNLGILVENLEPPMNIPE
    cDNA sequence of BN41901422 from canola (SEQ ID NO: 93):
    GTCATTCTTCTAATTTCTCTGACCTCTGCTACTGTCTATCCGTTCGTGTTGCTTTGATCT
    CTCTAATCAGACATGAAGAGAGGCAGCTTGAGTCTTAATCCCATCTCTCTCCCTCCTCC
    TGAGCAATCCATCTCCAAATTCTTAACACAGAGCGGAACGTTCAAGGATGGAGACCTT
    CAAGTGAACAAAGATGGAATCCAGACAGTATCTCATTCTGAGCCTGGAGCTCCACCAC
    CTATTGATCCATTGGACAACCAGTTGAGTTTGGCTGACCTTGAAGTGATCAAAGTCATT
    GGCAAAGGAAGCAGTGGTAGTGTTCAGCTGGTTAAACACAAACTAACTCAACAGTTTTT
    CGCTACTAAGGTTATTCAGTTAAACACAGAAGAGTCCACATGTCGAGCCATTTCTCAGG
    AGCTGAGGATAAACTTGGCATCTCAATGTCCATATCTCGTCTCATGTTATCAGTCTTTCT
    ACCATAACGGTCTCGTCTCAATCGTAATGGAGTTCATGGACGGTGGATCTCTTTTGGAT
    TTGTTGAAGAAAGTCCAGAGAGTTCCTGAAAACATGCTCGCTGCCATCTCCAAGCGAG
    TGCTCCGAGGCTTGTGCTATATTCACGATGAGAGGCGAATCATTCACCGGGACTTGAA
    GCCTTCCAACTTGCTAATCAATCACAGAGGTGAAGTCAAGATCGCAGACTTTGGTGTC
    AGCAAGATCTTGTCTAGCACAAGCAGTCTAGCGCATACCTTCGTGGGCACAGACTTCT
    ATATGTCGCCAGAGAGAATCAGTGGGAAAGCGTATGGGAACAAGTGTGATATTTGGAG
    TTTGGGAGTGGTTCTGCTCGAATGTGCAACGGGTAAGTTTCCGTATACTCCTCCTGAA
    AACATGAAGGGATGGACTAGCATGTATGAGCTAGTTGACGCCATTGTTGAAAACCCGC
    CTCCTCGTGCACCTTCCCACCTGTTCTCTCCAGAGTTTTGCTCCTTCATCTCGCAATGT
    GTACAAAAAGATCCAAGGGACCGGAAATCAGCAATGGAGCTTCTGGACCATAGGTTCG
    TAAACATGTTTGAAGATGTGGATGTGGATCTCTCGTCTTACTTCACCGCCGCAGGATCT
    TTGATTCCCCCACTAGCCAACAGCTAGAACCGAGTTTGAACAATCCTTTTAACACCAAG
    TTATATATATGTATTTTATATCCACTGGAAGAGACGATATTTACGAGATGTTGCGACTTA
    TGAGAGAATTCTCTTGATAGACATTTATATTTTCAAGTATTGAAATTTATTTGGGTAAAAA
    AAAAAAAAAA
    The BN41901422 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 94):
    MKRGSLSLNPISLPPPEQSISKFLTQSGTFKDGDLQVNKDGIQTVSHSEPGAPPPIDPLDN
    QLSLADLEVIKVIGKGSSGSVQLVKHKLTQQFFATKVIQLNTEESTCRAISQELRINLASQCP
    YLVSCYQSFYHNGLVSIVMEFMDGGSLLDLLKKVQRVPENMLAAISKRVLRGLCYIHDERRI
    IHRDLKPSNLLINHRGEVKIADFGVSKILSSTSSLAHTFVGTDFYMSPERISGKAYGNKCDIW
    SLGVVLLECATGKFPYTPPENMKGWTSMYELVDAIVENPPPRAPSHLFSPEFCSFISQCVQ
    KDPRDRKSAMELLDHRFVNMFEDVDVDLSSYFTAAGSLIPPLANS
    cDNA sequence of BN47868329 from canola (SEQ ID NO: 95):
    CCAGATCGTTAAACCATAATCCAAACCAAGCTTGCAAAAACTTTTGATCCTAAACCGAG
    ATGAAACCAATCCAACCGCCACCAGGAGTAATCGGTCCGGTTAAGAACCGCCCTCGC
    CGCCGTCCAGACCTCTCCTTACCACTTCCTCACCGCGACGTTTCCCTCGCCGTACCTC
    TCCCCCTCCCACCAACTTCCGGCGGCGGTTCCACCACCTCAGAGCCTAAAAGCTACTC
    AGACTTAGTACGTGGCAACCGGATCGGAAGCGGAGCCGGTGGAACGGTTTACAGAGT
    AGTCCACCGTCCAACCTCCCGCGTATACGCACTCAAGATAATCAACGGTAACCACGAT
    GACACTGTTCGTGGCCAGATCTGCAGAGAGATCAAGATTCTCCGAGACGTGAATCACC
    CCAACGTGGTGAAATGCCACGAGATGTTCGATCAAAACGGAGAGATCCAGGTCTTGCT
    CGAGCTCATGGACCAAGGATCTTTAGAAGGTGCTCATATCTCGAACGAGCAACAGTTA
    TCTGACCTATCTCGTCAGATACTAAACGGTTTGGCTTATCTTCACGGCCGTCATATAGT
    CCATAGAGACATAAAGCCATCGAATCTACTTATAAACTCGGACAATAACGTCAAGATTG
    CTGATTTTGGAGTGAGCAGGGTCTTGGCTCAGACCCTGTCTCCGTGTAAGTCCTCTGT
    TGGGACTATTGCTTACATGAGTCCTGAGAGGATCAACACGGATTTGAATCAGGGGATG
    TATGATGGTTGCGCTGGGGATATTTGGAGCTTCGGTGTTAGTGTTCTTGAGTTTTTCTT
    GGGGAGGTTTCCTTTTAATGTGAATAGGCTAGGTGATTGGGCTAGTCTTATGTGTGCTA
    TTTGTATGTCTAAGCCGCCTGAAGCTCCTGCCACGGCGTCTCCGGAGTTTAGACACTT
    TGTTTCGTGTTGTTTGCAGAGAGAACCGGGGAGGAGGCAAACTGCTGTTCAGCTTTTG
    CAACATCCTTTTGTGCGTAGAGGGGCGATTCAGAGTCAGAATAGGTCTCCTCAGAATC
    TACATCAACTCTTGCCTCCTCCACACTAAAGGTTTAGTTTTGTCTGATAATGTTTCTACA
    CTAAAGGTTGATCATGTCTTGCTGTTTAGACAAACTATATCATTGTCTTGTACTTAGCTG
    AAAGCAAAGCGTATATAGTTTGAATCACTTTGCACCTCATGATGGTTAATTTCACTAAGT
    AATTCAGTAGTAGAGTCATTAAATGTAAAAAAAAAAAAAAA
    The BN47868329 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 96):
    MKPIQPPPGVIGPVKNRPRRRPDLSLPLPHRDVSLAVPLPLPPTSGGGSTTSEPKSYSDLV
    RGNRIGSGAGGTVYRVVHRPTSRVYALKIINGNHDDTVRGQICREIKILRDVNHPNVVKCH
    EMFDQNGEIQVLLELMDQGSLEGAHISNEQQLSDLSRQILNGLAYLHGRHIVHRDIKPSNLL
    INSDNNVKIADFGVSRVLAQTLSPCKSSVGTIAYMSPERINTDLNQGMYDGCAGDIWSFGV
    SVLEFFLGRFPFNVNRLGDWASLMCAICMSKPPEAPATASPEFRHFVSCCLQREPGRRQT
    AVQLLQHPFVRRGAIQSQNRSPQNLHQLLPPPH
    cDNA sequence of BN42671700 from canola (SEQ ID NO: 97):
    CTGCAAACTAAAATCTAGAACCGGAACAGATCTAAACCAAACCAAACCGAACCGGGTG
    TCTTTGTTTGTAACTCTCCAAATGGTGAAGAAAGCGATGAAGGAGGAAGAAGAAGCAG
    AGATGAGAAACTCGTCGATGCAGTCAAAGTACAAAGGCGTGAGGAAGAGGAAGTGGG
    GCAAATGGGTTTCGGAGATCAGACTTCCCAACAGCAGAGAGCGAATCTGGCTAGGCT
    CTTTCGACACTCCCGAGAAGGCGGCGCGTGCCTTCGACGCCGCCCAGTTTTGTCTCC
    GCGGCTGCCAATCCGGTTTCAATTTCCCCGATAATCCGCCGTCGATCTCCGGCGGAA
    GGTCGCTGACGCCTCCGGAGATCCGGGAAGCGGCTGCTCGATACGCAAACGCTCAG
    GACGACGATATTATCATCACCACCGGAGAAGAAGAATCGGTTTTGTCCGAAACCCGAC
    CGGAGTCTCCTTCAACAACCTCCGTGTCTGAAGCAGATACGTCGCTGGATTGCGATCT
    ATCGTTCTTAGACACGCTTCCTAATGATTTCGGGATGTTTTCTGTGTTTGATGACTTCTC
    CGACGGCTTCTCCGGCGATCAGTTTACAGAGGTTTTACCCGTTGAAGATTACGGAGAT
    GTGATTTTTGATGAGTCTCTGTTTCTTTGGGATTTTTAAATGTGTAAAGAGTTTTGAATT
    GTTGTTTATTCGGGTCATGGAGAGTAATCTGGATATTTTTGTAAGTCGGAGCTCCAGCG
    ACCCGGGAACTTGATCATTCTTGCTTTGGTTGATGATATCTATCATTCCTTCATTTTTTG
    TTGTTATTAATGAAAATATTTGGATAAAATAGCAATTACAGAAAAAAAAAAAAAAAAAA
    The BN42671700 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 98):
    MVKKAMKEEEEAEMRNSSMQSKYKGVRKRKWGKWVSEIRLPNSRERIWLGSFDTPEKA
    ARAFDAAQFCLRGCQSGFNFPDNPPSISGGRSLTPPEIREAAARYANAQDDDIIITTGEEES
    VLSETRPESPSTTSVSEADTSLDCDLSFLDTLPNDFGMFSVFDDFSDGFSGDQFTEVLPVE
    DYGDVIFDESLFLWDF
    cDNA sequence of ZM68416988 from corn (SEQ ID NO: 99):
    CTCGCCTCGCCTTCCTCCGAGCCCCGGCGAGGAAGAGGAACCCGCCGCCGCCGCCG
    CCGGACGCACTTCCGATGGCGACGCCACGGAAGCCGATCAAGCTCACGCTGCCGTCC
    CACGAGACCACCATCGGCAAGTTCCTGACGCACAGCGGGACGTTCACGGACGGGGAT
    CTGCGCGTGAACAAGGACGGCCTCCGCATCGTCTCGCGGAGGGAGGGAGGCGAGGC
    TCCTCCTATAGAGCCGTTGGATAGTCAACTGAGCTTAGATGATCTAGACGTTATAAAAG
    TGATCGGGAAAGGTAGCAGCGGAAATGTGCAATTGGTCCGCCACAAATTTACTGGCCA
    GTTTTTTGCTCTGAAGGTTATTCAACTAAATATTGATGAGAGTATACGCAAACAGATTGC
    CAAGGAGTTGAAGATAAACTTATCAACACAGTGCCAATATGTTGTTGTGTTCTATCAGT
    GTTTCTATTTCAATGGTGCCATTTCTATTGTTTTGGAATACATGGATGGTGGCTCCCTTG
    CAGATTTCCTGAAGACTGTTAAAACCATTCCAGAGGCCTACCTCGCTGCTATCTGTACG
    CAGATGCTAAAAGGACTGATCTATTTGCATAACGAGAAGCGCGTTATACACCGAGATCT
    GAAACCATCAAATATATTGATAAATCATAGGGGTGAAGTAAAAATATCAGATTTTGGTGT
    GAGTGCCATTATATCTAGTTCCTCTTCGCAACGAGATACATTTATTGGCACACGCAACT
    ACATGGCGCCAGAAAGAATCGATGGAAAGAAACATGGTTCTATGAGTGATATCTGGAG
    TTTGGGACTAGTGATACTGGAATGTGCAACCGGCATCTTTCCATTTCCTCCTTGTGAAA
    GCTTCTACGAACTTCTCGTGGCTGTTGTTGATCAACCGCCACCTTCTGCGCCGCCGGA
    TCAGTTTTCACCAGAATTCTGTGGGTTCATTTCTGCATGTCTCCAGAAGGATGCTAATG
    ACAGGTCATCAGCCCAAGCCTTATTGGACCATCCGTTCCTGAGCATGTATGATGACCT
    GCATGTAGATCTTGCTTCGTACTTCACGACAGCAGGATCTCCTCTCGCCACCTTCAATT
    CCAGGCAACTCTAATTTTTTTGTCCTCCTTATTACGCGAACGGTGTGGCGACAAATTTC
    TCTTTTTGGACAAGGCTTGGATTGTGTACTGAGCTGTAATGATCTTGTGTGTGTCAGGT
    CGGTGATTGGCTCCATCACTTTACATATATGACATACATGTACAGCCTTTTAGGATAAA
    AATGAGCACTGAAGTTTTGCCTATCTGTATATCGGCAGCAAACGTTTGGTCATGTTTGT
    TTCACCTTGTAATGTATTGACTCAGATATGGGATTGGTCATTGTCTCTAAAAAAAAAAA
    The ZM68416988 cDNA is translated into the following
    amino acid sequence (SEQ ID NO: 100):
    MATPRKPIKLTLPSHETTIGKFLTHSGTFTDGDLRVNKDGLRIVSRREGGEAPPIEPLDSQL
    SLDDLDVIKVIGKGSSGNVQLVRHKFTGQFFALKVIQLNIDESIRKQIAKELKINLSTQCQYVV
    VFYQCFYFNGAISIVLEYMDGGSLADFLKTVKTIPEAYLAAICTQMLKGLIYLHNEKRVIHRD
    LKPSNILINHRGEVKISDFGVSAIISSSSSQRDTFIGTRNYMAPERIDGKKHGSMSDIWSLGL
    VILECATGIFPFPPCESFYELLVAVVDQPPPSAPPDQFSPEFCGFISACLQKDANDRSSAQA
    LLDHPFLSMYDDLHVDLASYFTTAGSPLATFNSRQL

Claims (4)

1-5. (canceled)
6. A transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a full-length polypeptide comprising SEQ ID NO: 35.
7. An Isolated polynucleotide comprising SEQ ID NO. 36.
8. A method of producing a transgenic plant, said method comprising the steps of:
a) introducing into a plant cell an expression vector comprising a polynucleotide comprising SEQ ID 35; and
b) generating from the plant cell a transgenic plant that expresses the polynucleotide.
US13/666,997 2007-07-13 2012-11-02 Transgenic Plants With Increased Stress Tolerance and Yield Abandoned US20130125255A1 (en)

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EP2520655A2 (en) 2012-11-07
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EP2520656A3 (en) 2013-05-01
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