US20130088129A1 - Laboratory Climatic Cabinet Having Improved Interior Humidification - Google Patents

Laboratory Climatic Cabinet Having Improved Interior Humidification Download PDF

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Publication number
US20130088129A1
US20130088129A1 US13/633,520 US201213633520A US2013088129A1 US 20130088129 A1 US20130088129 A1 US 20130088129A1 US 201213633520 A US201213633520 A US 201213633520A US 2013088129 A1 US2013088129 A1 US 2013088129A1
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US
United States
Prior art keywords
water
steam
steam generator
combination according
feed line
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US13/633,520
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English (en)
Inventor
Waldemar Pieczarek
Hermann Stahl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thermo Electron LED GmbH
Original Assignee
Thermo Electron LED GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Thermo Electron LED GmbH filed Critical Thermo Electron LED GmbH
Assigned to THERMO ELECTRON LED GMBH reassignment THERMO ELECTRON LED GMBH ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: PIECZAREK, WALDEMAR, STAHL, HERMANN
Publication of US20130088129A1 publication Critical patent/US20130088129A1/en
Abandoned legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L1/00—Enclosures; Chambers
    • B01L1/02—Air-pressure chambers; Air-locks therefor
    • B01L1/025—Environmental chambers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/10—Means to control humidity and/or other gases

Definitions

  • the present invention relates to a laboratory climatic cabinet, also referred to as an incubator, in particular, a gassed incubator, having an interior enclosed by a housing and a steam generator arranged outside the interior, which is connected to the interior via a steam feed line.
  • an incubator also referred to as an incubator, in particular, a gassed incubator, having an interior enclosed by a housing and a steam generator arranged outside the interior, which is connected to the interior via a steam feed line.
  • Incubators are typically used in laboratories for the purpose of storing samples, especially biological and/or microbiological samples, in their interior under predefined conditions such as a specific temperature and ambient humidity and—in the case of gassed incubators—a defined gas atmosphere.
  • a specific temperature and ambient humidity and—in the case of gassed incubators—a defined gas atmosphere.
  • the attempt is typically made to imitate the conditions of the human or animal body. Conditions which are often selected are therefore a temperature of approximately 37° C. and the highest possible ambient humidity, which is typically to be at least 60%, preferably at least 80%, particularly preferably at least 90%, without moisture condensing out on the walls or other areas of the incubator, however.
  • a first possibility is to provide a water reservoir in the interior of the incubator, from which water is vaporized by heating (for example, EP 1552888 A2).
  • a large problem of this solution is the easy microbial contamination of the water bath and the hazard of contamination of the samples stored in the incubator. This hazard of microbial contamination can be significantly reduced if superheated water steam is supplied to the interior of the incubator from the outside.
  • solutions are known in which an autoclave or sterilizer is set up outside the incubator, from which superheated water steam is fed under pressure into the incubator.
  • this solution is complex and costly.
  • these devices are subject to the safety requirements for pressure tanks, for example, the Ordinance on Industrial Safety and Health in Germany.
  • the object of the present invention is to devise such a laboratory climatic cabinet.
  • the present invention thus relates to a laboratory climatic cabinet, preferably a gassed incubator, having a housing, which encloses an interior, and a steam generator arranged outside the interior, which has a heating device for heating a water reservoir and for generating water steam, and is connected to the interior via a steam feed line.
  • the steam generator is connected via a water feed line to an unpressurized water container and the water container is arranged in such a manner that the water level in the water container during the steam generation is above the water level in the steam generator.
  • an external steam generator is provided in the present invention.
  • the presence of a contaminated water bath in the interior of the incubator is thus avoided.
  • the present invention differs from the external steam generators of the prior art, which feed superheated water steam under pressure to the interior of the incubator, in that the water container connected via the water feed line to the steam generator functions like an overpressure valve for the steam generator.
  • the hazard of steam explosions is therefore dispensed with, without restrictions occurring during the continuous provision of water steam.
  • the steam generator is also not subject to the relevant regulations for pressure tanks or boilers, for example, the German Ordinance on Industrial Safety and Health or its precursors, the Pressure Tank and Boiler Regulations. This makes production, approval, and maintenance significantly easier in comparison to typical steam pressure tanks.
  • the design according to the present invention allows an overpressure to be generated in the steam generator, which may additionally be set and uniformly regulated very easily through appropriate arrangement of the water container.
  • the maximum pressure which prevails in the steam generator results from the height of the water column which stands in the water container and water feed line, which suitably is an open line having no cocks or valves, above the water level of the steam generator.
  • the water container is thus expediently arranged relative to the steam generator so that a desired overpressure in the steam generator results during the steam generation because of the difference of the water levels.
  • a sufficient water column under the operating conditions and therefore a sufficient overpressure can be ensured by appropriate dimensioning, arrangement, and filling of the water container.
  • the water container is preferably arranged above the steam generator, it being sufficient if the heights of the bottom areas on which the respective water reservoir stands are different.
  • a sufficient pressure in the steam generator is typically achieved if the height difference of the water levels in the water container and the steam generator during the steam generation is at least 6 cm and preferably between 6 and 60 cm.
  • the height difference is particularly preferably 15 to 60 cm, and, in particular, it is between 20 and 30 cm.
  • the heating device for example, a plate heating body in the base region of the steam generator, preferably to boiling
  • an overpressure builds up in the interior of the steam generator.
  • a valve is expediently provided in the steam feed line to the incubator, which is only opened as needed to let steam into the interior of the incubator.
  • the regulation of the steam supply is performed in a way known per se based on the humidity and temperature values ascertained in the interior. If the values sink below a predefined target value, the valve is opened and steam flows into the interior until the predefined values are achieved.
  • the heating device of the steam generator can also be regulated in a typical manner, for example, by the controller of the incubator.
  • an electronic temperature controller is also possible, for example.
  • the heating device of the steam generator is expediently continuously operated in order to make water steam available continuously during the operation. Only phased operation of the heating device is theoretically also conceivable, however.
  • the water column which stands in the water feed line and in the water container is shifted in the direction toward the water container. It is expedient here if the water feed line leaves the water container in a base region and opens into a base region of the steam generator. Furthermore, the water heated in the steam generator meets colder water in the water feed line, thus cools down, and possibly entering steam condenses out. The pressure in the steam generator decreases because of the temperature reduction thus occurring. With appropriate layout and arrangement of the water container, the height difference of the water level in the steam generator and water container is sufficiently great so that the water standing in the water feed line is not displaced completely out of the line by the steam.
  • a pressure compensation opening can be provided in the container wall, which is preferably provided with a (sterile) filter.
  • the container could also be at least partially open on top. However, this is not preferable to avoid microbial contamination and from an energetic aspect. Rather, the container can expediently be provided with an insulation to avoid heat dissipation.
  • the water container To prevent contamination of the water reservoir, it is additionally preferable to also equip the water container with a heating device, in order to thus be able to heat the water reservoir. At least temporary heating to a temperature between 60° C. and below 100° C. is expedient. Below 60° C., no effect is achieved in regard to contamination avoidance or removal. In order to avoid boiling of the water reservoir in the water container, the temperature is also expediently kept below 100° C. The temperature is particularly preferably set to 70 to 90° C. and, in particular, to approximately 80° C. ( ⁇ 5° C.).
  • the heating of the water reservoir in the water container additionally has the advantage that the temperature increase of the water in the steam generator up to boiling is less than with non-preheated water.
  • the steam generator can therefore be designed as smaller and having lower performance.
  • the temperature setting is performed as in the steam generator, i.e., preferably by means of a PTC element.
  • the introduction of the water steam generated in the steam generator into the interior of the incubator is performed via a steam feed line which preferably rises from the steam generator to the opening into the interior of the incubator.
  • a steam feed line which preferably rises from the steam generator to the opening into the interior of the incubator.
  • the return line is led out of the steam feed line between the valve and the opening into the interior, this advantageously prevents water from standing in front of the valve and being entrained therefrom with the next steam introduction into the interior and sprayed therein.
  • it can additionally be insulated and/or heated, in particular, in the region between valve and incubator.
  • the present invention was described above in such a manner that one steam generator is provided for one incubator.
  • multiple incubators to one steam generator.
  • a valve is then expediently provided in each of the steam lines, which can be activated separately, so that the steam feed to each of the incubators can be set individually.
  • the use of only one steam generator for multiple incubators decreases the space requirement and the costs for the overall arrangement.
  • FIG. 1 shows a cross section through an incubator according to one embodiment of the present invention having connected steam generator.
  • FIG. 1 illustrates an incubator 1 according to one embodiment of the present invention.
  • the incubator 1 comprises a housing 2 , which encloses an interior 3 , in which, for example, microbiological samples can be stored under predefined temperature, humidity, and gas atmospheric conditions. Typical storage conditions for the samples are, for example, a temperature of 37° C. and an ambient humidity of approximately 95%.
  • the interior 3 is closable by a door (not visible in the cross section).
  • the internal equipment such as support floors, measuring devices, etc. has been left out for simplification.
  • a steam generator 4 is connected via a steam feed line 7 to the incubator 1 .
  • the steam generator 4 consists of a container having a base region 40 , which can accommodate a water reservoir 6 , and a steam chamber 42 located above it, designed here in the form of a cupola.
  • Water steam is generated in the steam generator 4 by heating the water reservoir 6 by means of a heating device 5 and fed from the steam chamber 42 via the steam feed line 7 , which leaves the steam generator at the highest point of the steam chamber 42 , to the interior 3 of the incubator 1 .
  • the steam feed line 7 is closed using a valve 71 , which is always opened in a way known per se when the humidity and temperature values measured in the interior 3 fall below predefined target values. After reaching the target values, the valve 71 is closed again and the steam supply is interrupted.
  • the steam generator 4 is connected via a water feed line 8 in the form of an unstopped tube, which leads out of its base region 40 , to a water container 9 and is arranged so that the base 41 of the steam generator 4 is located above the base 91 of the water container 9 .
  • the water feed line 8 leads out of a base region 90 of the water container 9 .
  • the water reservoir 6 is thus distributed to the steam generator 4 and the water container 9 . If the water reservoir 6 in the steam generator is heated by operating the heating device 5 , preferably to approximately 100° C., water steam forms and fills the steam chamber 42 . The pressure thus rises in the steam generator 4 , the water level 60 ′ sinks, and water is displaced out of the steam generator 4 via the water feed line 8 into the water container 9 .
  • FIG. 1 shows this, i.e., a state during the generation of water steam in the steam generator 4 .
  • the steam pressure in the steam generator 4 corresponds to the water column which stands above the water level 60 ′, i.e., the difference of the water levels 60 and 60 ′ (height h).
  • the arrangement of the water container 9 relative to the steam generator is such that under normal circumstances, the water standing in the water feed line 8 is not displaced completely out of the line. Steam which possibly penetrates into the water feed line 8 comes into contact with the colder water in the line 8 , cools down, and condenses out, whereby the pressure sinks. To equalize very high overpressures, a pressure compensation opening 13 is additionally provided in the water container 9 , through which the steam nonetheless possibly penetrating into the water container 9 can escape. Steam explosions are safely avoided in this manner.
  • the water reservoir 6 in the water container 9 fundamentally does not have to be heated at all.
  • a heating device 5 ′ is provided, using which the water reservoir 6 can also be heated in the water container 9 , although to a lower temperature than in the steam generator 4 . It is preferably heated to a temperature of at least 60° C., particularly preferably 80 to 90° C.
  • the higher the temperature of the water reservoir 6 the lower the probability that microbes will grow in the water, and the lower the heating power needed to bring water to a boil in the steam generator 4 .
  • the temperature of the water reservoir 6 in the water container 9 is not to be so high that water begins to boil therein, however.
  • the temperature is monitored using a PTC element 10 ′, which is connected (not shown here) to the power supply of the heating device 5 ′.
  • the temperature control in the steam generator 4 is performed similarly using a PTC element 10 .
  • the water container 9 is provided with an insulation 92 .

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Air Humidification (AREA)
  • Accommodation For Nursing Or Treatment Tables (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)
US13/633,520 2011-10-05 2012-10-02 Laboratory Climatic Cabinet Having Improved Interior Humidification Abandoned US20130088129A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102011114900.0 2011-10-05
DE102011114900.0A DE102011114900B4 (de) 2011-10-05 2011-10-05 Brutschrank mit verbesserter innenraum-befeuchtung

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US14/225,505 Continuation-In-Part US9572458B2 (en) 2012-10-30 2014-03-26 Multifunctional food processing tool for use with a food processing device

Publications (1)

Publication Number Publication Date
US20130088129A1 true US20130088129A1 (en) 2013-04-11

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ID=46875625

Family Applications (1)

Application Number Title Priority Date Filing Date
US13/633,520 Abandoned US20130088129A1 (en) 2011-10-05 2012-10-02 Laboratory Climatic Cabinet Having Improved Interior Humidification

Country Status (5)

Country Link
US (1) US20130088129A1 (zh)
EP (1) EP2578315B1 (zh)
CN (1) CN103028451B (zh)
DE (1) DE102011114900B4 (zh)
TR (1) TR201802504T4 (zh)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130146104A1 (en) * 2011-12-13 2013-06-13 Thermo Electron Led Gmbh Method For Cleaning The Usable Space Of A Climatic Cabinet
JP2019091675A (ja) * 2017-11-15 2019-06-13 栗田工業株式会社 リチウムイオン電池用ガス吸収材
CN111603971A (zh) * 2018-01-14 2020-09-01 杭州跟策科技有限公司 防扬尘防溅液的搅拌器

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102013008016A1 (de) 2013-05-08 2014-11-13 Thermo Electron Led Gmbh Laborgerät und/oder Medizinprodukt mit Anzeigevorrichtung für optoelektronischen Code und Betriebsverfahren dafür
EP3444329B1 (de) * 2017-08-14 2020-02-12 PeCon GmbH Vorrichtung zur anfeuchtung eines gasgemisches für die zellinkubation

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3619559A (en) * 1970-02-17 1971-11-09 Nat Camp Steam generator
US5524848A (en) * 1993-08-23 1996-06-11 Ellsworth; Scott P. Humidification process and apparatus
US5816496A (en) * 1996-04-25 1998-10-06 Kovacs; James E. Gas fired humidifier
US5949958A (en) * 1995-06-07 1999-09-07 Steris Corporation Integral flash steam generator
US6323464B1 (en) * 1998-11-16 2001-11-27 Robert J. Cohn Module for producing hot humid air for a proofing or holding operation
WO2003024633A1 (en) * 2001-09-20 2003-03-27 Thermsave Engineering Uk Limited Improved waste treatment
US20080138253A1 (en) * 2006-07-28 2008-06-12 Eschmann Holdsings Limited Autoclave
US20100150775A1 (en) * 2006-11-15 2010-06-17 Micropyretics Heaters International, Inc. Apparatus and method for sterilizing items

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US4701415A (en) * 1984-03-02 1987-10-20 Mallinckrodt, Inc. Controlled atmosphere enclosure
DE4130233C1 (zh) * 1991-09-09 1992-09-03 Melagapparate Gmbh & Co Kg, 1000 Berlin, De
US6024694A (en) * 1995-09-25 2000-02-15 Hill-Rom, Inc. Humidifier for a thermal support apparatus
DE19603509C1 (de) * 1996-02-02 1997-10-09 Voetsch Industrietechnik Gmbh Anordnung zur Erzeugung von Luftfeuchtigkeit in einem Prüfraum eines Umweltsimulationsgeräts sowie Verfahren zur Erzeugung einer gewünschten Luftfeuchtigkeit in einem solchen
DE69836683T2 (de) * 1998-10-05 2007-11-08 W & H Sterilization S.R.L., Pedrengo Autoklave
US6669626B1 (en) * 1999-12-23 2003-12-30 Hill-Rom Services, Inc. Humidifier for a patient support apparatus
AU2002343710A1 (en) * 2001-11-15 2003-06-10 Hill-Rom Services, Inc. Humidifier module
DE502004010672D1 (de) 2004-01-06 2010-03-11 Thermo Electron Led Gmbh Klimagerät mit keimdicht abgetrennten Bereichen
CN1298834C (zh) * 2004-11-11 2007-02-07 上海交通大学 大型海藻细胞或组织反应器预培养系统

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3619559A (en) * 1970-02-17 1971-11-09 Nat Camp Steam generator
US5524848A (en) * 1993-08-23 1996-06-11 Ellsworth; Scott P. Humidification process and apparatus
US5949958A (en) * 1995-06-07 1999-09-07 Steris Corporation Integral flash steam generator
US5816496A (en) * 1996-04-25 1998-10-06 Kovacs; James E. Gas fired humidifier
US6323464B1 (en) * 1998-11-16 2001-11-27 Robert J. Cohn Module for producing hot humid air for a proofing or holding operation
WO2003024633A1 (en) * 2001-09-20 2003-03-27 Thermsave Engineering Uk Limited Improved waste treatment
US20080138253A1 (en) * 2006-07-28 2008-06-12 Eschmann Holdsings Limited Autoclave
US20100150775A1 (en) * 2006-11-15 2010-06-17 Micropyretics Heaters International, Inc. Apparatus and method for sterilizing items

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
English language translation, Fuchise et al., JP10-142176A, 1998. Translated 11-27-2014. *
English language translation, Kim Jung Wee, KR10-0758665B1, 2007. Translated 11/28/2014. *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130146104A1 (en) * 2011-12-13 2013-06-13 Thermo Electron Led Gmbh Method For Cleaning The Usable Space Of A Climatic Cabinet
JP2019091675A (ja) * 2017-11-15 2019-06-13 栗田工業株式会社 リチウムイオン電池用ガス吸収材
CN111603971A (zh) * 2018-01-14 2020-09-01 杭州跟策科技有限公司 防扬尘防溅液的搅拌器

Also Published As

Publication number Publication date
DE102011114900B4 (de) 2015-08-27
CN103028451A (zh) 2013-04-10
EP2578315A1 (de) 2013-04-10
EP2578315B1 (de) 2018-01-17
TR201802504T4 (tr) 2018-03-21
CN103028451B (zh) 2015-03-11
DE102011114900A1 (de) 2013-04-11

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STCB Information on status: application discontinuation

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