US20100309547A1 - Objective-optical-system positioning apparatus and examination apparatus - Google Patents
Objective-optical-system positioning apparatus and examination apparatus Download PDFInfo
- Publication number
- US20100309547A1 US20100309547A1 US12/790,112 US79011210A US2010309547A1 US 20100309547 A1 US20100309547 A1 US 20100309547A1 US 79011210 A US79011210 A US 79011210A US 2010309547 A1 US2010309547 A1 US 2010309547A1
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- US
- United States
- Prior art keywords
- objective
- optical
- optical system
- examination
- positioning apparatus
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/0012—Surgical microscopes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/00147—Holding or positioning arrangements
- A61B1/00154—Holding or positioning arrangements using guiding arrangements for insertion
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/32—Devices for opening or enlarging the visual field, e.g. of a tube of the body
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/248—Base structure objective (or ocular) turrets
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/313—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor for introducing through surgical openings, e.g. laparoscopes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
Abstract
Description
- 1. Field of the Invention
- The present invention relates to an objective-optical-system positioning apparatus and an examination apparatus.
- This application is based on Japanese Patent Application No. 2009-133449, the content of which is incorporated herein by reference.
- 2. Description of Related Art
- Recently, it has become important to examine the condition of the inside, e.g., deep portions of the brain or organs, of biological specimens, such as small animals, while they are still alive (in vivo). Therefore, there is a known objective optical system and microscope system having a small-diameter end section, which enables in vivo time-sequential examination for a relatively long period of time by reducing the level of invasiveness on an organism (for example, Japanese Unexamined Patent Applications, Publication Nos. 2006-119300 and 2005-241671).
- When performing in vivo examination in the passage of time, to allow activity of the small animal, etc., which is similar to that during normal times, it is necessary to temporarily remove the objective optical system positioned at an examination site and then position the objective optical system at the same examination site again for examination. There is a known securing device that positions the objective optical system at this time with high reproducibility by inserting the objective optical system into en insertion hole of a support member secured to n organism (for example, Japanese Unexamined Patent Application, Publication No. 2005-253836).
- When the inside of an organism is to be examined with a low level of invasiveness on the organism and for a relatively long period of time by using both an objective optical system having a small-diameter end section and a securing device, it is desirable to keep the inner diameter of the insertion hole to a size slightly larger than the outer diameter of the small-diameter end section to decrease the level of invasiveness of the support member on the organism.
- Therefore, when the diameter of the insertion hole is decreased, the gap between the small-diameter end section and the insertion hole becomes very small when the small-diameter end section removed from the insertion hole is inserted again into the insertion hole.
- The present invention has been conceived in light of the above-described circumstances, and it is an object thereof to provide an objective-optical-system positioning apparatus and an examination apparatus teat enable a small-diameter end section of an objective optical system to be easily inserted into an insertion hole while preventing damage to the objective optical system and/or the support member, as well as any adverse effect on an organism, even when the insertion hole of the support member has a small diameter.
- To achieve the above-described object, the present invention provides the following solution.
- The present invention provides an objective-optical-system positioning apparatus including a positioning unit interposed between an objective optical system having a small-diameter end section and an examination optical system configured to examine light collected by the objective optical system; and a substantially cylindrical support unit, one end of which is secured to an organism, and which internally supports the small-diameter end section in a detachable manner, wherein the positioning unit includes a holding part for holding the objective optical system and a moving mechanism supporting the holding part such that the holding part is freely movable in a direction intersecting the direction of an optical axis of the objective optical system, and wherein the other end of the support unit has a tapered inner surface whose diameter gradually increases toward a tip thereof.
-
FIG. 1 is an overall configuration diagram of an objective-optical-system positioning apparatus and an examination apparatus according to an embodiment of the present invention. -
FIG. 2 is an overall configuration diagram illustrating a positioning unit of the objective-optical-system positioning apparatus inFIG. 1 . -
FIG. 3 is a front view of a microscope attachment part of the positioning unit inFIG. 2 . -
FIG. 4 is a front view of an intermediate part of the positioning unit inFIG. 2 . -
FIG. 5 is a front view of an objective attachment part of the positioning unit inFIG. 2 . -
FIG. 6 is a sectional side view illustrating a support unit of the objective-optical-system positioning apparatus inFIG. 1 . -
FIG. 7 is a diagram illustrating the usage of the objective-optical-system positioning apparatus inFig. 1 and illustrates a state in which the objective optical system is freely moveable. -
FIG. 8 is a diagram illustrating the usage of the objective-optical-system positioning apparatus inFIG. 1 and illustrates a state in which the optical axis of the objective optical system is being adjusted. -
FIG. 9 is a diagram illustrating the usage of the objective-optical-system positioning apparatus inFIG. 1 and illustrates a state when a specimen is moved close to the objective optical system. -
FIG. 10 is a diagram illustrating the process of inserting the objective optical system into an inserting hole using the objective-optical-system positioning apparatus inFIG. 1 . - An objective-optical-
system positioning apparatus 1 and anexamination apparatus 100 according to an embodiment of the present invention will be described below with reference toFIGS. 1 to 10 . In this embodiment, an example in which the inside of the brain of a live mouse (organism) A is examined will be described. - As illustrated in
FIG. 1 , the base of theexamination apparatus 100 according to this embodiment is an upright optical microscope. Theexamination apparatus 100 includes a microscope (examination optical system) 2 and the objective-optical-system positioning apparatus 1 according to this embodiment having apositioning unit 4, which is mounted on themicroscope 2 and to which an objectiveoptical system 3 is attached, and asupport unit 5, which is secured to the mouse A. - As the
microscope 2, a typical optical microscope or a laser scanning microscope is used. Themicroscope 2 has aunit attachment part 6 to which thepositioning unit 4 is attached. Theunit attachment part 6 has a structure typically used for mounting an objective optical system, and, for example, is provided with an RMS screw. By moving theunit attachment part 6 up and down along the direction of the optical axis, the positions of thepositioning unit 4 and the objectiveoptical system 3, which is attached to theunit attachment part 6, are adjusted in the height direction. The mouse A is placed on astage 7, which is movable on a horizontal plane, and the head region is secured to thestage 7 with asecuring member 8. - The objective
optical system 3 has a small-diameter end section 3 a. The objectiveoptical system 3 has an infinity design in which light collected from the tip of the objectiveoptical system 3 is transmitted to subsequent optical systems as a substantially parallel beam of light. - As illustrated in
FIG. 2 , thepositioning unit 4 is provided with amicroscope attachment part 9, anintermediate part 10, and an, objective attachment part (holding part) 11, in this order from themicroscope 2. AnX-axis guide 12 a (moving mechanism, linear guide) is interposed between themicroscope attachment part 9 and theintermediate part 10. A Y-axis guide 12 b (moving mechanism, linear guide) is interposed between theintermediate part 10 and theobjective attachment part 11. TheX-axis guide 12 a and the Y-axis guide 12 b are movable in directions orthogonal to each other on a plans orthogonal to the optical axis of themicroscope 2. As theguides objective attachment part 11 is movably supported with respect to themicroscope attachment part 9 on the horizontal plane. - As illustrated in
FIG. 3 , themicroscope attachment part 9 has amicroscope attachment screw 9 a that is removable from theunit attachment part 6 of themicroscope 2. By attaching themicroscope attachment screw 9 a to theunit attachment part 6, thepositioning unit 4 is mounted on themicroscope 2. A light-blockingcylinder 9 b, which extends near theobjective attachment part 11, is provided at substantially the center of themicroscope attachment part 9. In this way, light is blocked from entering from outside into the light path between themicroscope 2 and the objectiveoptical system 3, and stray light is prevented from being mixed into the illumination light from themicroscope 2 and the examination light collected by the objectiveoptical system 3. - As illustrated in
FIG. 4 , an intermediate-part hole 10 a through which the light-blockingcylinder 9 b penetrates is formed at substantially the center of theintermediate part 10. The intermediate-part hole 10 a has an inner diameter larger than the outer diameter of the light-blockingcylinder 9 b such that the movement of theintermediate part 10 is not restricted by the light-blockingcylinder 9 b when theintermediate part 10 moves in the horizontal direction with respect to themicroscope attachment part 9. - As illustrated in
FIG. 5 , an objectiveattachment screw hole 11 a, from which the rear section of the objectiveoptical system 3 is removable, penetrates through substantially the center of theobjective attachment part 11. - A microscope-side centering screw hole (optical-axis adjusting member) 14 a and an objective-side centering hole (optical-axis adjusting member) 14 b into which a centering screw (optical-axis adjusting member) 13 is inserted penetrate the
microscope attachment part 9 and theobjective attachment part 11, respectively, in the thickness direction thereof, at positions that align with the light-blockingcylinder 9 b and the objectiveattachment screw hole 11 a when they are disposed on substantially concentric axes. The centeringscrew 13 has ascrew part 13 a at the rear section and acone part 13 b, which is gradually tapered, at the tip section. Thescrew part 13 a is engaged with the microscope-side centeringscrew hole 14 a. Thecone part 13 b is inserted into the objective-side centering hole 14 b. - In this way, when a turning
screw 13 c provided at an intermediate position of the centeringscrew 13 is turned in a tightening direction, the position of theobjective attachment part 11 is adjusted to a position where the optical axis of the objectiveoptical system 3 and the optical axis of themicroscope 2 align. In addition, by providingnotches 10 b at the four corners of theintermediate part 10, theintermediate part 10 and the centeringscrew 13 are movable without their positions being restricted. - Furthermore, a securing screw hole (restricting member) 15 b with which a securing screw (restricting member) 15 a is engaged is formed in the
microscope attachment part 9. By tightening the securingscrew 15 a, ashim 16 interposed between thesecuring screw 15 a and themicroscope attachment part 9 is secured to themicroscope attachment part 9. In this way, theobjective attachment part 11 is secured to themicroscope attachment part 9 via acoupling member 17, which couples theshim 16 and theobjective attachment part 11. - The
support unit 5 is substantially cylindrical, and, as illustrated inFIG. 6 , has a small-diameter part 5 a, which is inserted into the organism, and a large-diameter part 5 b having a diameter larger than that of the small-diameter part 5 a. Inside the small-diameter part 5 a, aninsertion hole 5 c having an inner diameter that is slightly larger than the outer diameter of the small-diameter end section 3 a of the objectiveoptical system 3 extends in the longitudinal direction. At the bottom of theinsertion hole 5 c, awindow 5 d made of a transparent material, such as cover glass, is provided at the center section, which serves as a light path between the objectiveoptical system 3 and the specimen surface. - The tip of the small-
diameter part 5 a is blocked with atransparent member 5 e, such as glass, at a distance that creates an appropriate space between the bottom of theinsertion hole 5 c and the specimen surface. In this way, when the small-diameter end section 3 a is inserted into theinsertion hole 5 c until the surface at its tip bumps into the bottom of theinsertion hole 5 c, the objectiveoptical system 3 is appropriately focused at the specimen surface. - The large-
diameter part 5 b has aninner surface 5 f, which is a cone-shape that continues, with an increasing diameter, from the inner surface of theinsertion hole 5 c. - The use and operation of the objective-optical-
system positioning apparatus 1 and theexamination apparatus 100 having such configurations will be described below with reference toFIGS. 7 to 10 . InFIGS. 7 to 9 , parts of the configurations are omitted for the sake of simplifying the description of these configurations. - To carry out in vivo examination of the inside of the brain of the mouse A using the
examination apparatus 100 according to this embodiment, thesupport unit 5 is secured by bonding, with the small-diameter part 5 a inserted into a through-hole penetrating the skull of the mouse A, thereby securing the head of the mouse A to thestage 7. - First, by loosening the securing
screw 15 a, the objectiveoptical system 3 is set to a state in which it is freely movable on a substantially horizontal surface (refer toFIG. 7 ). Next, the centeringscrew 13 is tightened to adjust the optical axis of the objectiveoptical system 5 and the optical axis of themicroscope 2 to aligned positions. Subsequently, the securingscrew 15 a is tightened to secure the objectiveoptical system 3 to the microscope 2 (refer toFIG. 8 ), and, in this state, the centeringscrew 13 is loosened. At this time, the optical axis of the objectiveoptical system 3 is held at a position aligned with the optical axis of themicroscope 2. - Next, the
stage 7 is moved such that an insertion hole 15 c is disposed substantially vertically downward from the objectiveoptical system 3 to align the objectiveoptical system 3 and the examination site. Then, the securingscrew 15 a is loosened to set the objectiveoptical system 3 to a freely movable state (refer toFIG. 9 ), and the objectiveoptical system 3 is slowly moved downward to insert the small-diameter end section 3 a into theinsertion hole 5 c. - At this time, if the position of the objective
optical system 3 is misaligned relative to theinsertion hole 5 c, the tip of the small-diameter end section 3 a contacts the taperedinner surface 5 f (refer toFIG. 10 ). Then, the tip of the small-diameter end section 3 a smoothly moves along theinner surface 5 f, is guided to the position of theinsertion hole 5 c, and is directly inserted into theinsertion hole 5 c. After the small-diameter end section 3 a is inserted until it strikes the bottom of theinsertion hole 5 c, the securingscrew 5 a is tightened to fix the position of the objectiveoptical system 3. Through the above-described process, the inside of the brain of the mouse A can be examined in vivo using themicroscope 2. - In this way, according to this embodiment, the
inner surface 5 f, whose diameter gradually decreases toward theinsertion hole 5 c, is provided on thesupport unit 5. Furthermore, the objectiveoptical system 3 is inserted into theinsertion hole 5 c while being smoothly and freely movable in a direction intersecting the insertion direction. In this way, the position of the small-diameter end section 3 a is adjusted during the insertion process even when its position is misaligned. Therefore, there is an advantage in that, even when the inner diameter of theinsertion hole 5 c is decreased to substantially the same as the outer diameter of the small-diameter end section 3 a, the small-diameter end section 3 a can be easily inserted into theinsertion hole 5 c without requiring highly precise position control of the objectiveoptical system 3. - Furthermore, there is an advantage in that, by decreasing the diameter of the
insertion hole 5 c as much as possible in this way, the inside of the body of the mouse A can be examined over a relatively long period of time while reducing the invasiveness thesupport unit 5 on the mouse A. Moreover, even when the tip of the small-diameter end section 3 a contacts the taperedinner surface 5 f, the small-diameter end section 3 a smoothly moves in a direction that absorbs the impact due to the contact. In this way, there is an advantage in that damage to the small-diameter end section 3 a and thesupport unit 5 can be prevented, and, furthermore, any influence on the organism or examination site due to the impact at contact can be prevented. - Furthermore, even when the configuration of the objective
optical system 3 is such that it is freely movable in a direction intersecting the optical axis, the position of the objectiveoptical system 3 is fixed relative to themicroscope 2 when required through a simple method. In this way, there is an advantage in that it is possible to prevent inconveniences such as the optical axis being misaligned due to the objectiveoptical system 3 moving after adjusting the optical axis, and furthermore, a more stable image can be acquired by preventing blurriness of the field of view during examination. - In the above-described embodiment, the
unit attachment part 6 is movable in the direction of the optical axis of the objectiveoptical system 3. Instead, however, thestage 7 may be movable in the direction of the optical axis of the objectiveoptical system 3. - Even in this way, the same advantages as those according to the above-described embodiment can be achieved.
- Moreover, in the above-described embodiment, the
upright microscope 2 is used. Instead, however, an inverted microscope may be used. - Furthermore, in the above-described embodiment, the
microscope attachment part 9 is attached to themicroscope 2 by themicroscope attachment screw 9 a with a structure that is the same as that of the mount of the objectiveoptical system 3. Instead, however, themicroscope 2 may be attached by a structure that is the same as that of a revolver mount. - Furthermore, in the above-described embodiment, instead of the objective
optical system 3, a revolver may be attached to theobjective attachment part 11. By doing so, a plurality of objectiveoptical systems 3 each having a different specification may be attached to the revolver, which is attached to theobjective attachment part 11, and examination can be performed while easily changing the magnification, etc. - Furthermore, in the above-described embodiment, the angle between the objective
optical system 3 and thestage 7 may be adjustable. In this case, thestage 7 may be tilted, and the optical system including the objectiveoptical system 3 and thepositioning unit 4 may be tilted. - It is also possible to dispose the
support unit 5 secured to the head of the mouse A such that the longitudinal direction of theinsertion hole 5 c is tilted with respect to the optical axis of the objectiveoptical system 3. Accordingly, by adjusting the angle between thestage 7 and the objectiveoptical system 3 such that the longitudinal direction of theinsertion hole 5 c is aligned with the direction of the optical axis of the objectiveoptical system 3, the small-diameter end section 3 a can be easily inserted into theinsertion hole 5 c from an appropriate direction. - At this time, for example, in a state in which the specimen is secured to the
stage 7, a rod-like member having a length approximately the same as that of the objectiveoptical system 3 or an object having the same shape as that of the outer cylinder of the objectiveoptical system 3 may be inserted into theinsertion hole 5 c, and the angle between thestage 7 and the objectiveoptical system 3 may be adjusted such that the center axes thereof are aligned with the optical axis of themicroscope 2. In this way, the angle between thestage 7 and the objectiveoptical system 3 can be easily adjusted. Instead, a reflective member, e.g., mirror, which reflects light, may be attached at a position where theinsertion hole 5 c is covered. In such a case, selecting an angle that maximizes the intensity of an image examined by themicroscope 2, the angle between thestage 7 and the objectiveoptical system 3 can be easily adjusted.
Claims (10)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2009-133449 | 2009-06-02 | ||
JP2009133449A JP5393264B2 (en) | 2009-06-02 | 2009-06-02 | Objective optical system position adjustment device and observation device |
Publications (2)
Publication Number | Publication Date |
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US20100309547A1 true US20100309547A1 (en) | 2010-12-09 |
US8405903B2 US8405903B2 (en) | 2013-03-26 |
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Application Number | Title | Priority Date | Filing Date |
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US12/790,112 Expired - Fee Related US8405903B2 (en) | 2009-06-02 | 2010-05-28 | Objective-optical-system positioning apparatus and examination apparatus |
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US (1) | US8405903B2 (en) |
JP (1) | JP5393264B2 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20210169334A1 (en) * | 2019-12-05 | 2021-06-10 | Regents Of The University Of Minnesota | Systems and methods for multimodal neural sensing |
US11197735B2 (en) | 2015-11-05 | 2021-12-14 | Inscopix, Inc. | Systems and methods for optogenetic imaging |
US11733501B2 (en) | 2015-09-02 | 2023-08-22 | Inscopix Inc. | Systems and methods for color imaging |
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US5295477A (en) * | 1992-05-08 | 1994-03-22 | Parviz Janfaza | Endoscopic operating microscope |
US5496261A (en) * | 1993-07-30 | 1996-03-05 | Carl-Zeiss-Stiftung | Combination of a viewing and/or documenting apparatus and an endoscope as well as a method of operating the combination |
US5588949A (en) * | 1993-10-08 | 1996-12-31 | Heartport, Inc. | Stereoscopic percutaneous visualization system |
US20070097494A1 (en) * | 2003-10-17 | 2007-05-03 | Olympus Corporation | Objective lens unit, objective lens insertion tool, microscope, objective optical system fixing device, and microscope system |
US7304789B2 (en) * | 2004-02-24 | 2007-12-04 | Olympus Corporation | Microscope system and objective unit |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2501098B2 (en) * | 1984-06-25 | 1996-05-29 | オリンパス光学工業株式会社 | microscope |
JP4579563B2 (en) * | 2004-03-15 | 2010-11-10 | オリンパス株式会社 | Objective optical system fixing device |
JP2005241671A (en) | 2004-02-24 | 2005-09-08 | Olympus Corp | Microscope system and object unit |
JP4504153B2 (en) | 2004-10-20 | 2010-07-14 | オリンパス株式会社 | Immersion objective optical system |
-
2009
- 2009-06-02 JP JP2009133449A patent/JP5393264B2/en not_active Expired - Fee Related
-
2010
- 2010-05-28 US US12/790,112 patent/US8405903B2/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5295477A (en) * | 1992-05-08 | 1994-03-22 | Parviz Janfaza | Endoscopic operating microscope |
US5496261A (en) * | 1993-07-30 | 1996-03-05 | Carl-Zeiss-Stiftung | Combination of a viewing and/or documenting apparatus and an endoscope as well as a method of operating the combination |
US5588949A (en) * | 1993-10-08 | 1996-12-31 | Heartport, Inc. | Stereoscopic percutaneous visualization system |
US20070097494A1 (en) * | 2003-10-17 | 2007-05-03 | Olympus Corporation | Objective lens unit, objective lens insertion tool, microscope, objective optical system fixing device, and microscope system |
US7304789B2 (en) * | 2004-02-24 | 2007-12-04 | Olympus Corporation | Microscope system and objective unit |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11733501B2 (en) | 2015-09-02 | 2023-08-22 | Inscopix Inc. | Systems and methods for color imaging |
US11197735B2 (en) | 2015-11-05 | 2021-12-14 | Inscopix, Inc. | Systems and methods for optogenetic imaging |
US11690696B2 (en) | 2015-11-05 | 2023-07-04 | Inscopix, Inc. | Systems and methods for optogenetic imaging |
US20210169334A1 (en) * | 2019-12-05 | 2021-06-10 | Regents Of The University Of Minnesota | Systems and methods for multimodal neural sensing |
Also Published As
Publication number | Publication date |
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JP5393264B2 (en) | 2014-01-22 |
US8405903B2 (en) | 2013-03-26 |
JP2010279437A (en) | 2010-12-16 |
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