US20090075952A1 - Nitroderviatives as drugs for diseases having an inflammatory basis - Google Patents

Nitroderviatives as drugs for diseases having an inflammatory basis Download PDF

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US20090075952A1
US20090075952A1 US12/271,440 US27144008A US2009075952A1 US 20090075952 A1 US20090075952 A1 US 20090075952A1 US 27144008 A US27144008 A US 27144008A US 2009075952 A1 US2009075952 A1 US 2009075952A1
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acid
ester
formula
nitrooxymethyl
acetyloxy
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Piero del Soldato
Francesca Benedini
Patrizia Antognazza
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Nicox SA
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Nicox SA
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Assigned to NICOX S.A. reassignment NICOX S.A. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: ANTOGNAZZA, PATRIZIA, BENEDINI, FRANCESCA, DEL SOLDATO, PIERO
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/24—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with substituted hydrocarbon radicals attached to ring carbon atoms
    • C07D213/28—Radicals substituted by singly-bound oxygen or sulphur atoms
    • C07D213/30—Oxygen atoms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C203/00—Esters of nitric or nitrous acid
    • C07C203/02—Esters of nitric acid
    • C07C203/04—Esters of nitric acid having nitrate groups bound to acyclic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C233/00—Carboxylic acid amides
    • C07C233/01—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms
    • C07C233/16—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms
    • C07C233/24—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by a carbon atom of a six-membered aromatic ring
    • C07C233/25—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by a carbon atom of a six-membered aromatic ring having the carbon atom of the carboxamide group bound to a hydrogen atom or to a carbon atom of an acyclic saturated carbon skeleton
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C317/00—Sulfones; Sulfoxides
    • C07C317/44—Sulfones; Sulfoxides having sulfone or sulfoxide groups and carboxyl groups bound to the same carbon skeleton
    • C07C317/46—Sulfones; Sulfoxides having sulfone or sulfoxide groups and carboxyl groups bound to the same carbon skeleton the carbon skeleton being further substituted by singly-bound oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C323/00—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups
    • C07C323/50—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton
    • C07C323/51—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
    • C07C323/60—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton with the carbon atom of at least one of the carboxyl groups bound to nitrogen atoms

Definitions

  • the present invention relates to compounds and the use thereof for diseases affecting the digestive apparatus, in particular the intestinal tract, specifically colites, gastrites, enterites, duodenites and hepatopathies of various nature (on a viral, immune, dismetabolic basis due to intoxications from drugs such as paracetamol and other analgesic, antibiotic, antitumoural, antidepressive drugs, etc., alcohol, etc.).
  • the digestive apparatus diseases are very diffused. While the therapy of the peptic ulcer has generally reached efficacy, the same cannot be said for other diseases affecting the digestive apparatus. For example it is known that yearly in the Unites States more than 25 million people suffer diseases affecting liver and gall-bladder and more than 26,000 people die owing to chronic hepatopathies and cirrhosis. Generally the therapeutical treatment is widely unsatisfactory. Among the compounds used for these treatments interferon ⁇ -2b can be mentioned, which allows the recovery in about 30-40% of the cases affected by chronic hepatitis B and 20-25% of those affected by chronic hepatitis C.
  • the treatment is above all of support and it can consist in a suitable diet, alcohol abstinence or in the administering of diuretics or vitamins.
  • the therapeutic treatment is generally unsatisfactory for the diseases affecting the intestinal tract such colites, duodenites, enterites.
  • diseases affecting the intestinal tract such colites, duodenites, enterites.
  • 5-amino salicylic acid and derivatives thereof is not fully effective.
  • steroidal compounds for example prednisolone and the like
  • pathologies on an inflammatory basis such as those above described affecting the digestive apparatus, are considered precancerous forms, since they can evolve into tumoral processes.
  • pathologies on an inflammatory basis which can concern different systems such the urogenital, respiratory apparatuses, the skin districts, etc.
  • An object of the present invention is the use, for diseases on an inflammatory basis, of nitroderivatives or salts thereof having the following general formula (I):
  • the diseases on an inflammatory basis are those particularly affecting the digestive apparatus, preferably the intestinal tract, such as for example colites, gastrites, enterites, duodenites; besides epatopathies and tumoral processes related to diseases on an inflammatory basis.
  • Y 3 in formula (II) of the linking group Y of X 1 in formula (I) is selected from the following bivalent radicals:
  • Y 3 is an aromatic ring having 6 atoms, containing one nitrogen atom, said aromatic ring having the two free valences respectively in the positions 2 and 6, or 2 and 3 or 2 and 5 with respect to the heteroatom.
  • Y 3 is Y12 (pyridyl) substituted as above indicated.
  • the bonds can also be in an unsymmetrical position, for example Y12 (pyridyl) can be substituted also in position 2 and 3; Y1 (pyrazol) can be 3,5-disubstituted.
  • the drug radical has formula (AI) and the compounds of formula (I) are the following:
  • the drug radical has formula AII and the compounds of formula (I) are the following:
  • the bivalent radical precursors of formula (II) are for example those wherein the two free valences are saturated with two hydroxyl groups, or with one hydroxyl group and one carboxylic group. These compounds are available on the market.
  • the drug radical R or the bivalent radical Y and/or W as above defined contain one or more asymmetric carbon atoms
  • the corresponding precursors can be used in the synthesis of the compounds of the invention both in racemic form and as single optical isomers.
  • a salifiable functional group for example an amino or heterocyclic nitrogen is present
  • the corresponding salts are obtained by reaction in organic solvent such as for example acetonitrile, tetrahydrofuran, with an equimolecular amount of the corresponding organic or inorganic acid.
  • Examples of usable organic acids are the following: oxalic, tartaric, maleic, succinic, citric acid.
  • Examples of usable inorganic acids are the following: nitric, hydrochloric, sulphuric, phosphoric acid. Nitric and hydrochloric acid are preferred.
  • the compounds of the invention develop a marked protective action towards hepatopathies and in general towards diseases affecting the digestive apparatus, in particular the intestinal tract, in particular colites, gastrites, enterites, duodenites and hepatopathies. It has been found that the compounds of the invention in comparison with native precursors not only are not toxic as to the digestive apparatus, but unexpectedly they are able to prevent or reduce the diseases affecting this apparatus.
  • the paracetamol efficacy as analgesic is known, however this compound causes damages at hepatic level (hepatic toxicity).
  • the paracetamol nitrooxy derivatives according to the invention besides being effective analgesic drugs, have no hepatic toxicity, but they are also able to prevent or reduce already existing hepatic damages.
  • the compounds of the invention have a beneficial action also on tumoral processes, when used in the prophylaxis or in the therapy.
  • the pathologies on an inflammatory basis are considered precancerous forms, being able to subsequently evolve into tumoral processes.
  • the pathologies on an inflammatory basis can involve various systems such as the urogenital, respiratory, skin, digestive system, etc.
  • the compounds of the invention can be used alone or in combination with known antitumoral treatments, such for example the administering of chemotherapeutic drugs, for example cis-platinum, adriamycin etc., or the radiotherapeutic treatment.
  • chemotherapeutic drugs for example cis-platinum, adriamycin etc.
  • radiotherapeutic treatment for example the radiotherapeutic treatment.
  • the administering of the compounds of the invention can be made contemporaneously with the chemotherapeutic or radiotherapeutic treatments, or previously or subsequently to the chemotherapeutic or radiotherapeutic treatments.
  • the compounds of the invention are used for the treatment and/or prevention of the tumoral process affecting the digestive apparatus.
  • the preferred compounds are the above ones.
  • the compounds of the invention are prepared according to known methods of the prior art.
  • Acyl halides are prepared according to known procedures of the prior art, for example by thionyl or oxalyl chloride, halides of P III or P V in solvents inert under the reaction conditions, such for example toluene, chloroform, DMF, etc.
  • the compounds of the present invention are formulated in the corresponding pharmaceutical compositions for parenteral, oral and topical use according to the well known techniques in the field, together with the usual excipients; see for example the volume “Remington's Pharmaceutical Sciences 15a Ed.”
  • the amount on a molar basis of the active principle in these formulations is the same, or lower, with respect to that used as antiinflammatory and/or analgesic drug of the corresponding precursor drug.
  • the daily administrable doses are those of the antiinflammatory and/or analgesic precursor drugs, or, in case, lower.
  • the daily doses can be found in the literature of the field, such as for example in “Physician's Desk reference”.
  • the 2-acetyloxybenzoic acid 6-(nitrooxymethyl)-2-methyl pyridinyl ester nitrate is obtained starting from the 2-acetyloxybenzoic acid 6-(nitrooxymethyl)-2-methylpyridinyl ester isolated at step C) of Example 1.
  • the 2-acetyloxybenzoic acid 5-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride is synthesized according to the process described in Example 1, starting from acetyl salicylic acid and 2,5-bis(chloromethyl)pyridine.
  • the compound is synthesized according to the process described in Example 1 A) starting from 2,5-pyridin-dimethanol, synthesized in its turn by reduction with NaBH 4 of di-ethyl-2,5-pyridin dicarboxylate in ethanol as described in patent JP 48029783.
  • the 2-acetyloxybenzoic acid 3-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride is synthesized according to the process described in Example 1, starting from acetyl salicylic acid and 2,3-bis(chloromethyl)pyridine.
  • the compound is synthesized according to the process described in Example 1A) starting from 2,3-pyridin dimethanol, synthesized in its turn by reduction with LiAlH 4 of di-methyl-2,3-pyridinedicarboxylate in ethanol as described in J. Chem. Soc., Perkin Trans. 1 (1972), (20), 2485-2490.
  • 3-hydroxymethylphenol (10 g, 0.08 moles) is dissolved in toluene (50 ml) containing triethylamine (9.8 g, 0.1 moles).
  • the organic phases are mixed together, they are washed with a 5% carbonate solution (150 ml ⁇ 2) and then with water (125 ml ⁇ 2).
  • the organic phase is anhydrified with sodium sulphate in the presence of decolorating carbon. It is filtered under vacuum and the solvent is evaporated under reduced pressure and at a bath temperature lower than 40° C., obtaining 48.2 g of 2-(acetyloxy)benzoic acid 4-(formyl)phenyl ester.
  • the reaction raw product is used without further purification.
  • the organic phase is washed with a 5% sodium bicarbonate solution and then with water. It is anhydrified with sodium sulphate and the solvent is evaporated at reduced pressure and the residue is used without further purification.
  • the raw product is purified by chromato-graphy on silica gel eluting with hexane/ethyl acetate 3/7 (v/v ratio). 1 g of 3-[(2-iodo)ethyloxy]propanoic acid 4-acetylamino phenyl ester is obtained.
  • the organic phase is anhydrified with sodium sulphate and the solvent is evaporated at reduced pressure.
  • the reaction raw product is purified by chromatography on silica gel using as eluent hexane/ethyl acete (9/1 v/v) to give 2-hydroxybenzoic acid 3-(nitrooxmethyl)phenylester (0.8 g). Yield 46%.
  • the compound is synthesized according to the process described in Example 16.
  • the process total yield is 32%.
  • the substance appears as an amorphous solid.
  • reaction mixture is kept under stirring at room temperature for 8 hours, ethyl acetate is added and the mixture is washed with water.
  • the organic phase is anhydrified with sodium sulphate and the solvent is evaporated at reduced pressure.
  • the reaction raw product is purified by chromatography on silica gel eluting with a mixture of hexane/ethyl acetate (3/7 v/v).
  • Cis-5-fluoro-2-methyl-1-[[4-(methylsulphinyl)phenyl]methylene]-1H-indene-3-acetic acid 4-bromobutyl ester (3.8 g) is obtained as a yellow solid. Yield 55%.
  • the (Z)-5-fluoro-2-methyl-1-[[4-(methylsulphinyl)phenyl]methylene]-1H-indene-3-acetic acid 6(nitrooxymethyl)-2-methyl pyridinyl ester is synthesized according to the process described in Example 1, starting from (Z)-5-fluoro-2-methyl-1-[[4-(methylsulphinyl)phenyl]methylene]-1H-indene-3-acetic acid and 2,6-bis(chloromethyl)pyridine. Total yield of the process 20%.
  • the 2-acetyloxybenzoic acid 2-(nitrooxymethyl)phenyl ester is synthesized according to the process described in Example 6, starting from acetylsalicylic acid and 2-hydroxybenzaldehyde. Total yield of the process 68%.
  • the animals (rats of Swiss stock weighing about 20 g) are divided in groups of at least No. 10 animals for group.
  • the animals receive concanavalin and solvent (treated control group), solvent (polyethylene glycol 400—untreated control group), concanavalin and tested compound dissolved in the solvent (treated groups).
  • Rats are treated intravenously with concanavalin A (0.3 mg/rat), and after 5 minutes they receive by intraperitoneal injection the tested compounds, at the doses reported in Table 1, dissolved in polyethyleneglycol 400.
  • HT29 Human adenocarcinoma (HT29) cells taken from colon affected by cancerous process were transferred into plates with 24 wells containing a cellular culture medium formed by 10% of foetal bovine serum, penicillin (50. U/ml), streptomycin (50 mg/ml) and PEG 400 (polyethyleneglycol). After 24 hours a part of the plates is inoculated with the tested compounds dissolved in the carrier (PEG 400). 96 hours after the inoculation of the compounds the cellular growth was measured by haemocytometer. The results, reported in Table 2, are expressed as percentage of the cellular proliferation with respect to the controls.
  • the experiment was carried out by using three human epithelial cellular lines of the prostate cancer (PNT1A; LLNCaP; PC3) and three human epithelial cellular lines of the bladder cancer (T24; 647V; 1207), the various types of cellular lines are identified on the basis of the characteristics, in particular of the aggressiveness, of the cancerous process.
  • the cancerous cells are sown, with an initial concentration of 20,000 cells/cm 2 , in plates having 96 wells with a cellular culture medium RPMI added with foetal bovine serum 5% and L-Glutamine 1%. Solutions in dimethylsulphoxide of the tested compounds at three different concentrations (10 ⁇ 6 M; 10 ⁇ 5 M; 10 ⁇ 4 M) or the carrier (DMSO 1 0 / 00 ) are added to the culture medium. 4 days after the treatment the cellular growth was measured by the method with MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) de-scribed by Turner in: Turner T., Chen P., Goodly L. J., Wells A.
  • MTT 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
  • Human adenocarcinoma cells are sown on plates having 24 wells (2.5 ⁇ 10 5 cells/plate) with a standard culture medium.
  • the cell monolayer of each plate is first washed twice with a cold saline buffer, then treated with TCA (trichloroacetic acid) at 5% for 10 minutes and then washed three times with absolute alcohol.
  • TCA trichloroacetic acid
  • the cells of each well are dissolved in 500 ⁇ l of NaOH 0.1N and the incorporated radioactivity is determined by scintallation counting.
  • Cisplatinum Cisplatinum Controls 438 100 Carrier (DMSO) — 438 100 Salicylic acid 200 438 100 Comparison 3-(nitrooxymethyl) 200 246 50 phenyl ester of Salicylic acid (Ex. 15) Acetylsalicylic acid 200 438 100 Comparison 3-(nitrooxymethyl) 200 192 46 phenyl ester of Acetylsalicylic acid (Ex. 5)

Abstract

Use for the treatment of diseases having an inflammatory basis of compounds or salts thereof, having the following general formula (I): A-X1-L-(W)p-NO2 wherein A contains the radical of a drug, X1 and W are bivalent radicals, L is a covalent bond or oxygen, sulphur, NR1c wherein R1c is H or a C1-C5 linear or branched alkyl.

Description

  • The present invention relates to compounds and the use thereof for diseases affecting the digestive apparatus, in particular the intestinal tract, specifically colites, gastrites, enterites, duodenites and hepatopathies of various nature (on a viral, immune, dismetabolic basis due to intoxications from drugs such as paracetamol and other analgesic, antibiotic, antitumoural, antidepressive drugs, etc., alcohol, etc.).
  • The digestive apparatus diseases are very diffused. While the therapy of the peptic ulcer has generally reached efficacy, the same cannot be said for other diseases affecting the digestive apparatus. For example it is known that yearly in the Unites States more than 25 million people suffer diseases affecting liver and gall-bladder and more than 26,000 people die owing to chronic hepatopathies and cirrhosis. Generally the therapeutical treatment is widely unsatisfactory. Among the compounds used for these treatments interferon α-2b can be mentioned, which allows the recovery in about 30-40% of the cases affected by chronic hepatitis B and 20-25% of those affected by chronic hepatitis C.
  • However the interruption of the treatment causes a recidivism in 50-80% of the patients. Only 10% of the cases of hepatitis B are satisfactory with interferon α-2b. Another compound used for these pathologies is ribavirin, however the efficacy is not yet well known. Other used compounds are vaccines, which however are used only in the prophylaxis.
  • For the cirrhosis treatment there are generally no effective compounds. At present the treatment is above all of support and it can consist in a suitable diet, alcohol abstinence or in the administering of diuretics or vitamins.
  • The therapeutic treatment is generally unsatisfactory for the diseases affecting the intestinal tract such colites, duodenites, enterites. For example the therapy with 5-amino salicylic acid and derivatives thereof is not fully effective. The use of steroidal compounds (for example prednisolone and the like) can cause toxic symptoms or serious side effects.
  • It must be added that generally the pathologies on an inflammatory basis, such as those above described affecting the digestive apparatus, are considered precancerous forms, since they can evolve into tumoral processes. In the same way for the pathologies on an inflammatory basis, which can concern different systems such the urogenital, respiratory apparatuses, the skin districts, etc.
  • Therefore the treatment of these pathologies of inflammatory nature has a critical importance also in the prevention and in the treatment of tumoral diseases.
  • The need was felt to have available compounds active in diseases on an inflammatory basis, in particular those affecting the digestive apparatus and for the prevention and/or treatment of the tumoral processes related to the above diseases.
  • It has been surprisingly found by the Applicant that it is possible to solve the above technical problem with specific nitroderivatives as described hereunder.
  • An object of the present invention is the use, for diseases on an inflammatory basis, of nitroderivatives or salts thereof having the following general formula (I):

  • A-X1-L-(W)p-NO2  (I)
  • wherein:
      • p is an integer equal to 1 or 0;
      • A=R-T1-, wherein
        • R is the radical of a precursor drug and it has the following formulas:
  • Figure US20090075952A1-20090319-C00001
        • s is an integer and is 1 or 0;
        • RAI is H, CH3;
        • R1 is OCOR3, R3 being a C1-C5 linear or branched radical, NHCOR3, wherein R3 has the above meaning, or R1 is OH, CH2CH(CH3)2, phenyl, benzoyl, 4,6-dichlorophenylamino;
        • R6 is H, or an halogen atom, preferably fluorine; or R1 and R6, when are located in the adjacent positions 4 and 5 of the aromatic ring of formula (AI), form the radical of following formula (AIa):
  • Figure US20090075952A1-20090319-C00002
        • or R can be the following formula:
  • Figure US20090075952A1-20090319-C00003
        • T1=(CO)t or (X)t′, wherein X=O, S, NR1C, R1C is H or a linear or branched allyl having from 1 to 5 carbon atoms, t and t′ are integers and equal to zero or 1, with the proviso that t=1 when t′=0; t=0 when t′=1;
      • X1=-TB-Y-TBI- wherein
        • TB and TBI are equal or different;
        • TB=(CO) when t=0, TB=X when t′=0, X being as above;
        • TBI=(CO)tx or (X)txx, wherein tx and txx have the 0 or 1 value; with the proviso that tx=1 when txx=0; and tx=0 when txx=1; X is as above;
        • Y is a bivalent linking group selected from the following:
  • Figure US20090075952A1-20090319-C00004
          • wherein:
          • nIX is an integer in the range 0-3, preferably 1;
          • nIIX is an integer in the range 1-3, preferably 1;
          • RTIX, RTIX′, RTIIX, RTIIX′, equal to or different from each other are H or a C1-C4 linear or branched alkyl; preferably RTIX, RTIX′, RTIIX, RTIIX′ are H;
          • Y3 is a saturated, unsaturated or aromatic heterocyclic ring having 5 or 6 atoms, containing one or two nitrogen atoms,
          • an alkylene group R′ wherein R′ is a C1-C20 linear or branched when possible, preferably having from 2 to 6 carbon atoms, optionally substituted with one or more of the following groups: —NHCOR3, wherein R3 is as above, —NH2, —OH or
          • a cycloalkylene having from 5 to 7 carbon atoms, optionally substituted with side chains R′, R′ being as above, one or more carbon atoms of the cycloalkylene ring can optionally be substituted by heteroatoms; or
  • Figure US20090075952A1-20090319-C00005
          • wherein n3 is an integer from 0 to 3 and n3′ is an integer from 1 to 3;
  • Figure US20090075952A1-20090319-C00006
          • wherein n3 and n3′ have the above meaning,
  • Figure US20090075952A1-20090319-C00007
          • wherein
          • R4 is hydroxy, hydrogen, R5O— alkoxy wherein R5 is a C1-C10 linear or branched or cyclic alkyl group, preferably R5 is a methyl group; R2 is a C2-C10 linear or branched alkenylene group which can contain one or more double bonds, preferably R2 is the ethenylene group (—CH═CH—); or
  • Figure US20090075952A1-20090319-C00008
          • wherein R1f=H, CH3 and nf is an integer from 0 to 6; preferably from 0 to 4;
      • L=covalent bond, or L=X, X being as above, or L=CO;
      • W=YTO wherein YT has the same meanings of Y but in the compound of formula (I) YT is equal to or different from Y. Preferably YT is different from Y.
  • The diseases on an inflammatory basis are those particularly affecting the digestive apparatus, preferably the intestinal tract, such as for example colites, gastrites, enterites, duodenites; besides epatopathies and tumoral processes related to diseases on an inflammatory basis.
  • When in formula (AI), R1 is an acetyloxy group in position 2 of the ring, s=0 and R6=H and the free valence of the radical R is saturated with the —COOH group, the compound is known as Acetylsalicylic Acid,
  • when in formula (AI) R1 is an hydroxyl group in position 2 of the ring, s=0 and R6=H and the free valence of the radical R is saturated with a —COOH group, the compound is known as Salicylic Acid,
  • when in formula (AI) R1 is an acetylamino group in position 4 of the ring, s=0 and R6=H and the free valence is saturated with an —OH group, the compound is known as Paracetamol,
  • when in formula (AI) R1 is CH2CH(CH3)2 in position 4 of the ring, s=1, RAI=CH3 and R6=H and the free valence is saturated with a —COOH group, the compound is known as Ibuprofen,
  • when in formula (AI) R1 is phenyl and it is in position 4 of the ring, s=1, RAI=CH3 and R6=F in position 3 and the free valence is saturated with a —COOH group, the compound is known as Flurbiprofen,
  • when in formula (AII) the free valence is saturated with the —COOH group, the compound is known as Sulindac;
  • when in formula (AI) R1 and R6 are the radical of formula (AIa) and they are connected with the positions 4 and 5 of the ring, s=1, RAI=CH3, R6=H and the free valence is saturated with a —COOH group, the compound is known as Naproxen;
  • when in formula (AI) R1 is a benzoyl radical in position 5 of the aromatic ring, s=1, RAI=CH3, R6=H and the free valence is saturated with a —COOH group, the compound is known as Ketoprofen;
  • when in formula (AI) R1=2,6-dichlorofenilammino in position 2 of the ring, s=1, RAI=H, R6=H and the free valence is saturated with a —COOH group, the compound is known as Diclofenac.
  • Preferably Y3 in formula (II) of the linking group Y of X1 in formula (I) is selected from the following bivalent radicals:
  • Figure US20090075952A1-20090319-C00009
    Figure US20090075952A1-20090319-C00010
  • Preferably Y3 is an aromatic ring having 6 atoms, containing one nitrogen atom, said aromatic ring having the two free valences respectively in the positions 2 and 6, or 2 and 3 or 2 and 5 with respect to the heteroatom.
  • The preferred of Y3 is Y12 (pyridyl) substituted as above indicated. The bonds can also be in an unsymmetrical position, for example Y12 (pyridyl) can be substituted also in position 2 and 3; Y1 (pyrazol) can be 3,5-disubstituted.
  • The preferred compounds are those wherein in formula (I):
      • when in formula (AI) s=Q and R6=H:
        • R is a radical of formula (AI) wherein the substituent R1 is in position 2 of the aromatic ring, and it is selected between acetyloxy or hydroxyl, or it is an acetylamino group and then it is in position 4; -T1-TB- is a —CO—O— or —O—OC— ester group; Y of the radical X1 is a bivalent linking group selected from the following:
          • a radical of formula (III) as above, wherein n3=0 and n3′=1,
          • a radical of formula (II) as above wherein Y3 is Y12 as above defined,
          • a radical of formula (VIII) as above wherein R1f is hydrogen and nf=1;
        • TB1=—O—, L=covalent bond; p=0;
        • R is a radical of formula (AI) wherein the substituent R1 is in position 2 of the aromatic ring, and it is selected between acetyloxy or hydroxyl, or it is an acetylamino group and then it is in position 4; -T1-TB- is a —CO—O— or —O—OC— ester group; Y of the radical X1 is a bivalent linking group having formula (V) as above wherein R4 is a methoxyl group and R2=—CH═CH—; -TB1-L- is a —CO—O— or —O—OC— ester group; p=1; W=YO wherein Y is —(CH2)4— or —(CH2)3—;
        • R is a radical of formula (AI) wherein the substituent R1 is in position 4 of the aromatic ring, and it is acetylamino; -T1-TB-=—O—CO—; Y of the radical X1 is —(CH2)3—; -TB1-L-=—O— (L=covalent bond); p=0;
        • R is a radical of formula (AI) wherein the substituent R1 is in position 4 of the aromatic ring, and it is acetylamino; -T1-TB-=—O—CO—; Y of the radical X1 is an ethylene group substituted with an acetylamino group: —CH(NHCOCH3)—CH2—; -TB1-L-=—S—CO—; p=1; W=YO wherein Y is —(CH2)3—;
      • when in the formula (AI) s=1:
        • R is a radical of Formula (AI), R6=H or F in position 3 of the ring, R1=CH2CH(CH3)2 or phenyl in position 4, -T1-TB- is a —CO—O— ester group; Y of the radical X1 is a bivalent linking group having formula (V) as above wherein R4 is a methoxyl group and R2=—CH═CH—; -T1-LB- is a —CO—O— ester group; p=1; W=YO wherein Y is —(CH2)3—;
      • when in formula (I) R is a radical of formula (AII), -T1-TB-=—CO—O—; Y of the radical X1 is a bivalent linking group selected from the following:
        • a radical of formula (II) as above wherein Y3 is Y12 as above,
        • —(CH2)4—;
        • -TB1-=—O—, L=covalent bond; p=0.
  • The preferred compounds according to the present invention are those wherein:
  • the drug radical has formula (AI) and the compounds of formula (I) are the following:
    • 2-(acetyloxy)benzoic acid 3-(nitrooxymethyl)phenyl ester,
    • 2-(hydroxy)benzoic acid 3-(nitrooxymethyl)phenyl ester,
    • 2-(acetyloxy)benzoic acid 4-(nitrooxymethyl)phenyl ester,
    • 2-(hydroxy)benzoic acid 4-(nitrooxymethyl)phenyl ester,
    • 2-(acetyloxy)benzoic acid 2-(nitrooxymethyl)phenyl ester,
    • 2-(hydroxy)benzoic acid 2-(nitrooxymethyl)phenyl ester,
    • 2-(acetyloxy)benzoic acid 6-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride, or nitrate,
    • 2-(hydroxy)benzoic acid 6-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride, or nitrate,
    • 2-(acetyloxy)benzoic acid 5-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride, or nitrate,
    • 2-(hydroxy)benzoic acid 5-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride, or nitrate,
    • 2-(acetyloxy)benzoic acid 3-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride, or nitrate,
    • 2-(hydroxy)benzoic acid 3-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride, or nitrate,
    • trans-3-[4-[2-acetyloxybenzoyloxy]-3-methoxyphenyl]-2-propenoic acid 4-(nitrooxy)butyl ester,
    • trans-3-[4-[2-hydroxybenzoyloxy]-3-methoxyphenyl]-2-propenoic acid 4-(nitrooxy)butyl ester,
    • 4-(nitrooxy)butanoic acid 4-(acetylamino)phenyl ester,
    • trans-3-[4-(4′-nitrooxybutyryloxy)-3-methoxyphenyl]-2-propenoic acid 4-(acetylamino)phenyl ester,
    • 3-(nitrooxymethyl)-benzoic acid 4-(acetylamino)phenyl ester,
    • 4-(nitrooxymethyl)-benzoic acid 4-(acetylamino)phenyl ester,
    • 2-(nitrooxymethyl)-benzoic acid 4-(acetylamino)phenyl ester,
    • 5-(nitrooxymethyl)pyridin-2-carboxylic acid 4-(acetyl amino)phenyl ester,
    • 6-(nitrooxymethyl)-pyridin-2-carboxylic acid 4-(acetyl amino)phenyl ester,
    • 3-(nitrooxymethyl)-pyridin-2-carboxylic acid 4-(acetylamino)phenyl ester,
    • 5-(nitrooxymethyl)-pyridin-2-carboxylic acid 4-(acetylamino)phenyl ester,
    • 5-(nitrooxymethyl)pyridin-2-acetic acid 4-(acetylamino)phenyl ester,
    • 6-(nitrooxymethyl)pyridin-2-acetic acid 4-(acetylamino)phenyl ester,
    • 3-(nitrooxymethyl)pyridin-2-acetic acid 4-(acetylamino)phenyl ester,
    • 3-[(2-nitrooxy)ethyloxy]propanoic acid 4-(acetylamino)phenyl ester,
    • trans 3-[4-(41-nitrooxybutyryloxy)-3-methoxy]phenyl-2-propenoic acid 4-(acetylamino)phenyl ester,
    • 2-(acetylamino)-3-(4-nitrooxybutyryl)-3-mercaptopropanoic acid 4-(acetylamino)phenyl ester,
    • trans-3-[4-[α-methyl-4-(2-methylpropyl)phenylacetyloxy]-3-methoxyphenyl]-2-propenoic acid 4-nitrooxybutyl ester,
    • trans 3-[4-[2-fluoro-α-methyl(1,1′-biphenylyl)-acetyloxy]-3-methoxyphenyl]-2-propenoic acid 4-nitrooxybutyl ester,
    • (S) 6-metoxy-α-methyl-2-naphtalenacetic acid 2-methoxy-4-[(1E)-3-[4-(nitrooxy)butoxy]-3-oxo-1-propenyl]phenyl ester,
    • (S) 6-metoxy-α-methyl-2-naphtalenacetic acid 3-(nitrooxy methyl)phenyl ester,
    • (S) 6-metoxy-α-methyl-2-naphtalenacetic acid 6-(nitrooxy methyl)-2-methylpyridinil ester,
    • (S,S)-N-acetyl-S-(6-metoxy-α-methyl-2-naphtaleneacetyl) cysteine 4-(nitrooxy)butyl ester,
    • 2-[(2,6-dichlorophenyl)amino]benzeneacetic acid 6-(nitro oxymethyl)-2-methylpyridinil ester chloridrate,
  • The drug radical has formula AII and the compounds of formula (I) are the following:
    • (z)-5-fluoro-2-methyl-1-[[4-(methylsulphinyl)phenyl]methylene]-1H-inden-3-acetic acid 4-(nitrooxy)butyl ester,
    • (z)-5-fluoro-2-methyl-1-[[4-(methylsulphinyl)phenyl]methylene]-1H-inden-3-acetic acid 6-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride, or nitrate,
    • (Z)-5-fluoro-2-methyl-1-[[4-(methylsulphinyl)phenyl]methylene]-1H-inden-3-acetic acid 5-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride, or nitrate,
    • (Z)-5-fluoro-2-methyl-1-[[4-(methylsulphinyl)phenyl]methylene]-1H-inden-3-acetic acid 3-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride, or nitrate.
  • Other precursors of the general formula A=R-T1- wherein the free valence is saturated with —OH, that can be used for obtaining the compounds of formula (I) are the following:
  • (S)-Benzenepropanoic acid, 4-[2-(2-benzoxazolylmethyl amino)ethoxy]-.-(2-ethoxy) of formula (XX):
  • Figure US20090075952A1-20090319-C00011
  • (S)-Benzenepropanoic acid, 4-[2-(2-benzoxazolylmethyl amino)ethoxy]-.-(2,2,2-trifluoroethoxy) of formula (XXI):
  • Figure US20090075952A1-20090319-C00012
  • Compounds (XX) and (XXI) are described in PCT Patent Application WO 97/25042;
  • L-Tyrosine, N-(2-benzoylphenyl)-O-[2-(methyl-2-pyridinyl amino)ethyl] of formula (XXII):
  • Figure US20090075952A1-20090319-C00013
  • The above compound is described in PCT Patent Application WO 97/31907;
  • Prosta-5,9,12,14-tetraen-1-oic acid, 11-oxo-, (5Z,12E, 14E) (15-Deoxy Δ12,14-prostaglandin) of formula (XXIII):
  • Figure US20090075952A1-20090319-C00014
  • (2S,5S)-4-(4-(4-carboxyphenyl)butyl)-2-heptyl-4-oxo-5-thiazolidine N,N-dibenzylacetamide of formula (XXIV):
  • Figure US20090075952A1-20090319-C00015
  • The above compound is described in Proc. Natl. Acad. Sci 1999, 96(11), 6102-6106.
  • The bivalent radical precursors of formula (II) are for example those wherein the two free valences are saturated with two hydroxyl groups, or with one hydroxyl group and one carboxylic group. These compounds are available on the market.
  • When the drug radical R or the bivalent radical Y and/or W as above defined contain one or more asymmetric carbon atoms, the corresponding precursors can be used in the synthesis of the compounds of the invention both in racemic form and as single optical isomers.
  • When in the molecule of the compounds of the invention (formula I) a salifiable functional group, for example an amino or heterocyclic nitrogen is present, it is possible to use the corresponding salts. The latter are obtained by reaction in organic solvent such as for example acetonitrile, tetrahydrofuran, with an equimolecular amount of the corresponding organic or inorganic acid.
  • Examples of usable organic acids are the following: oxalic, tartaric, maleic, succinic, citric acid.
  • Examples of usable inorganic acids are the following: nitric, hydrochloric, sulphuric, phosphoric acid. Nitric and hydrochloric acid are preferred.
  • The compounds of the invention, as said, develop a marked protective action towards hepatopathies and in general towards diseases affecting the digestive apparatus, in particular the intestinal tract, in particular colites, gastrites, enterites, duodenites and hepatopathies. It has been found that the compounds of the invention in comparison with native precursors not only are not toxic as to the digestive apparatus, but unexpectedly they are able to prevent or reduce the diseases affecting this apparatus. For example the paracetamol efficacy as analgesic is known, however this compound causes damages at hepatic level (hepatic toxicity). The paracetamol nitrooxy derivatives according to the invention, besides being effective analgesic drugs, have no hepatic toxicity, but they are also able to prevent or reduce already existing hepatic damages.
  • The results obtained with the compounds of the invention are still more surprising if one considers that by using another NO donor such for example sodium nitroprussiate in pathologies affecting the digestive apparatus, there is no protection, on the contrary an hepatic damage occurs. Besides, this drug causes high hypotension.
  • As said, the compounds of the invention have a beneficial action also on tumoral processes, when used in the prophylaxis or in the therapy. As said, the pathologies on an inflammatory basis are considered precancerous forms, being able to subsequently evolve into tumoral processes. The pathologies on an inflammatory basis can involve various systems such as the urogenital, respiratory, skin, digestive system, etc.
  • Therefore the treatment of these pathologies of inflammatory nature has a critical importance also in the prevention and in the treatment of tumoral diseases.
  • In the treatment of tumoral diseases the compounds of the invention can be used alone or in combination with known antitumoral treatments, such for example the administering of chemotherapeutic drugs, for example cis-platinum, adriamycin etc., or the radiotherapeutic treatment.
  • It has unexpectedly been found by the Applicant that the compounds of the invention, when used in combination with the above tumoral treatments, synergically enhance the therapeutic effect.
  • The administering of the compounds of the invention can be made contemporaneously with the chemotherapeutic or radiotherapeutic treatments, or previously or subsequently to the chemotherapeutic or radiotherapeutic treatments.
  • Preferably the compounds of the invention are used for the treatment and/or prevention of the tumoral process affecting the digestive apparatus. The preferred compounds are the above ones.
  • The compounds of the invention are prepared according to known methods of the prior art.
  • In general if in the drug molecule or in the molecules of the radicals Y and W more reactive groups such as for example COOH and/or HX are present, they must be protected before the reaction according to the known procedures of the prior art; for example as described in the volume by Th. W. Greene: “Protective groups in organic synthesis”, Harward University Press, 1980.
  • Acyl halides are prepared according to known procedures of the prior art, for example by thionyl or oxalyl chloride, halides of PIII or PV in solvents inert under the reaction conditions, such for example toluene, chloroform, DMF, etc.
    • 1) When in formula (I) L is a covalent bond and p=0, and the free valence of the drug radical R is saturated with a carboxylic group, the synthesis methods for obtaining the corresponding nitrooxyderivatives are the following:
    • 1.a) The acyl halide of the drug of formula R—CO—Cl is reacted with an halogenalcohol of formula HO—Y-Hal, wherein Y is as above and Hal is halogen (Cl, Br, I).

  • R—COCl+HO—Y-Hal→R—CO—O—Y-Hal  (1A)
    • 1.b) Alternatively, the reaction can be carried out by reacting the sodium or potassium salt of the drug with a dihalogen derivative of general formula Y(Hal)2, wherein Y and Hal are as above defined.

  • R—COONa+Hal-Y-Hal→R—CO—O—Y-Hal  (1B)
    • 1.c) Or the drug of formula RCOOH is treated with an agent activating the carboxyl selected from N,N′carbonyl diimidazole (CDI), N-hydroxybenzotriazole and dicyclohexylcarbodiimide in solvent such for example, DMF, THF, chloroform etc. at a temperature in the range −5° C.-50° C. and reacted in situ with a compound HO—Y-Hal, wherein Y and Hal are as above defined.
  • Figure US20090075952A1-20090319-C00016
    • 1.d) Alternatively, the acyl halide of the drug is reacted with a compound HO—Y—OH, wherein Y is as above, in the presence of a base, in an organic solvent inert under the reaction conditions according to the scheme reported hereunder:
  • Figure US20090075952A1-20090319-C00017
    • 1.e) Alternatively to the previous syntheses the acyl halide of the drug is reacted with a compound HO-M-CHO, wherein M is an aromatic ring having 6 carbon atoms, or a radical Y3 as above, in the presence of a base, in an organic solvent inert under the reaction conditions according to the scheme reported hereunder:
  • Figure US20090075952A1-20090319-C00018
    •  The obtained compound is subjected to hydrogenation in the presence of palladium on carbon to give the corresponding alcohol:
  • Figure US20090075952A1-20090319-C00019
    •  wherein Y is as above defined.
    • 1.f) When the products obtained in the above reactions have formula R—COO—Y-Hal the corresponding nitrooxyderivatives are obtained by reacting the compound R—CO—O—Y-Hal with AgNO3 in organic solvent such as acetonitrile, tetrahydrofuran according to the scheme:
  • Figure US20090075952A1-20090319-C00020
    • 1.g) When the compounds obtained in the above reactions have formula R—COO—Y—OH the corresponding nitrooxyderivatives can be obtained by treatment with fuming nitric acid in organic solvent under anhydrous conditions and in inert atmosphere, in the presence of an inorganic acid different from the nitric acid, or with an organic acid, or of an anhydride of one or two organic acids.
    • 1.h) Alternatively, in the compound of formula R—COO—Y—OH the hydroxyl group is subjected to halogenation, for example, with PBr3, PCl5, SOCl2, PPh3+I2, and then reacted with AgNO3 in organic solvent such as acetonitrile, tetrahydrofuran.
    • 2) When in formula (I) L is a covalent bond and p=0, and the free valence of the radical R of the drug is saturated with a hydroxyl group, the synthesis methods for obtaining the corresponding nitrooxyderivatives are the following:
    • 2.a) By reaction of the drug of formula R—OH with an acyl halide of formula Hal-Y—COHal, wherein Y and Hal are as above, according to the scheme:
  • Figure US20090075952A1-20090319-C00021
    • 2.b) By reaction of the drug of formula R—OH with an acyl halide of formula OH—Y—COHal, wherein Y and Hal are as above, according to the scheme:
  • Figure US20090075952A1-20090319-C00022
    • 2.c) When the compounds obtained in the above reactions have formula R—OCO—Y-Hal or R—OCO—Y—OH the corresponding nitrooxyderivatives are obtained as described in 1.f and 1.h respectively.
    • 3. When in formula (I) p=1 and L=X, wherein X is as above, or L=CO, and the free valence of the radical R of the drug is saturated with a carboxylic group, the synthesis methods for obtaining the corresponding nitrooxyderivatives are the following:
    • 3.a) By reaction between the acyl halide of the drug and the compound of formula HX—Y—COOH, wherein X and Y are as above defined, according to the known methods of the prior art, to give the compound R—CO—X—Y—COOH which is transformed into the corresponding sodium salt and reacted with a compound of formula Hal-YT-R8 wherein Hal and YT are as above and R8 is Cl, Br, Iodine, OH:

  • R—COHal+HX—Y—COOH→R—CO—X—Y—COOH  (3.A)

  • R—CO—X—Y—COONa+Hal-YT—R8→R—CO—X—Y—CO—YT—R8  (3.A′)
    •  If R8=OH the compound of formula (3.A′) is subjected to halogenation as described in 1.h); if R8=Hal the compound of formula (3.A′) is reacted with AgNO3 in organic solvent such as acetonitrile, tetrahydrofuran:
    • 3.b) When YT is a C4 linear alkylene, the acid of formula (3.A) is reacted with triphenylphosphine in the presence of an halogenating agent such as CBr4 or N-bromosuccinimide in tetrahydrofuran to give the compound of formula (3.A′) wherein R8=Br which is transformed into the corresponding nitrooxyderivative as described in 1.h.
    • 4) When in formula (I) p=1 and L=X or CO, and the free valence or the radical R of the drug is saturated with an hydroxyl group, the synthesis methods for obtaining the corresponding nitrooxyderivatives are the following:
    • 4.a) Reaction of the drug of formula R—OH with an acyl halide of formula HX—Y—COHal, wherein X and Y are as above defined, according to the known methods of the prior art, to give the compound R—O—CO—Y—XH which is reacted with a compound of formula R8—YT—CoHal wherein R8 and YT are as above.

  • R—OH+HX—Y—COCl→R—O—CO—Y—XH  (4.A)

  • R—O—CO—Y—XH+R8—YTCO-Hal→R—O—CO—Y—X—CO—YT—R8  (4A′)
    • 4.b) Alternatively, the drug of formula R—OH is reacted with a compound of formula HX—Y—COOH, wherein X and Y are as above, in the presence of dicyclohexylcarbodiimide as described in 1.c, to give the compound R—O—CO—Y—XH, which is reacted with a compound of formula R8—YT—COCl wherein R8 and YT are as above defined to give the following compound: R—O—CO—Y—X—CO—YT-R8 (4.B).
    •  When R8=OH the compound of formula (4.B) or of formula (4A′) is subjected to halogenation as described in 1.h); if R8=Hal the compound of formula (4.B) is reacted with AgNO3 in organic solvent such as acetonitrile, tetrahydrofuran.
  • The compounds of the present invention are formulated in the corresponding pharmaceutical compositions for parenteral, oral and topical use according to the well known techniques in the field, together with the usual excipients; see for example the volume “Remington's Pharmaceutical Sciences 15a Ed.”
  • The amount on a molar basis of the active principle in these formulations is the same, or lower, with respect to that used as antiinflammatory and/or analgesic drug of the corresponding precursor drug.
  • The daily administrable doses are those of the antiinflammatory and/or analgesic precursor drugs, or, in case, lower. The daily doses can be found in the literature of the field, such as for example in “Physician's Desk reference”.
  • The following Examples illustrate the invention and they are not limitative of the scope of the same.
    • EXAMPLES Example 1 Synthesis of 2-acetyloxybenzoic acid 6-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride of Formula
  • Figure US20090075952A1-20090319-C00023
    • A) Synthesis of 2,6-bis-(chloromethyl)pyridine
  • To thionyl chloride (11.6 ml, 158 mmoles), cooled at 0° C., 2,6-bis-(hydroxymethyl)pyridine (4 g, 28 mmoles) is added very slowly. The obtained solution is left under stirring for 2 hours at room temperature, then the thionyl chloride in excess is evaporated at a reduced pressure. The obtained residue is treated with chloroform and it is evaporated again at a reduced pressure to remove the thionyl chloride residues. The raw product is treated with chloroform and washed with water. The organic phase is anhydrified with sodium sulphate and dried obtaining 4.81 g of the product as white solid having melting point=76-78° C.
    • B) Synthesis of 2-acetyloxybenzoic acid 6-(chloromethyl)-2-methylpyridinyl ester
  • To a solution of acetylsalicylic acid (1.6 g, 8.88 mmoles) in N,N′-dimethylformamide (20 ml) and under stirring sodium ethylate (0.64 g, 8.88 mmoles) is added. After 30 minutes the obtained solution is added to a solution of 2,6-bis-(chloromethyl)pyridine (4.72 g, 26.81 mmoles) in N,N′-dimethylformamide (20 ml). The solution is left at room temperature for 7 days, under stirring, then it is diluted with ethyl ether and washed with water. The separated organic phases are anhydrified with sodium sulphate and the solvent is evaporated at a reduced pressure. The reaction raw product is purified by chromatography oh silica gel eluting with n-hexane/ethyl acetate 7/3. 1.7 g of the product as yellow oil are obtained.
  • 1H-NNR (200 MHz) (CDCl3): 8.10 (1H, d); 7.74 (1H, t); 7.57 (1H, t); 7.42 (1H, d); 7.33 (2H, m); 7.11 (1H, d); 5.42 (2H, s); 4.67 (2H, s); 2.41 (3H, s).
    • C) Synthesis of 2-acetyloxybenzoic acid 6-(nitrooxymethyl)-2-methylpyridinyl ester
  • To a solution of 2-acetyloxybenzoic acid 6-(chloromethyl)-2-methylpyridinyl ester (1.5 g, 4.7 mmoles) in acetonitrile (20 ml) kept under stirring, silver nitrate (1.3 g, 7.65 mmoles) is added. The solution is heated up to 80° C., maintaining it sheltered from light, under stirring for 30 hours. The formed silver chloride is filtered, the solvent is evaporated. The reaction raw product is purified by chromatography on silica gel eluting with n-hexane/ethyl acetate 7/3. 1.2 g of product as yellow oil are obtained.
  • 1H-NMR (200 MHz) (CDCl3): 8.10 (1H, d); 7.74 (1H, t); 7.57 (1H, t); 7.42 (1H, d); 7.33 (2H, m); 7.11 (1H, d); 5.60 (2H, s); 5.42 (2H, s); 2.41 (3H, s).
    • D) Synthesis of 2-acetyloxybenzoic acid 6-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride
  • To a solution of 2-acetyloxybenzoic acid 6-(nitrooxymethyl)-2-methylpyridinyl ester (1 g, 2.88 mmoles) in ethyl acetate (20 ml) cooled at 0° C., a solution of ethyl acetate/HCl 5M is added dropwise under stirring. It is left for 1 hour at 0° C., then the temperature is let reach room values. The formed precipitate is filtered and washed with ethyl ether. 900 mg of solid product are obtained.
    • Elementary Analysis
  • Calculated C 50.21% H 3.95% N 7.31% Cl 9.26%
    Found C 50.23% H 3.97% N 7.29% Cl 9.20%
  • 1H NMR (200 MHz) (CDCl3): 8.10 (2H, m); 7.7 (1H, t); 7.56 (2H, d); 7.48 (1H, t); 7.30 (1H, d); 5.74 (2H, s); 5.43 (2H, s); 2.20 (3H, s).
    • Example 2 Synthesis of 2-acetyloxybenzoic acid 6-(nitrooxymethyl)-2-methylpyridinyl ester nitrate of Formula
  • Figure US20090075952A1-20090319-C00024
  • The 2-acetyloxybenzoic acid 6-(nitrooxymethyl)-2-methyl pyridinyl ester nitrate is obtained starting from the 2-acetyloxybenzoic acid 6-(nitrooxymethyl)-2-methylpyridinyl ester isolated at step C) of Example 1.
  • To a solution of 2-acetyloxybenzoic acid-6-(nitrooxymethyl)-2-methylpyridinyl ester (1 g, 2.88 mmoles) in acetonitrile (10 ml) cooled at 0° C., a solution of nitric acid 65% (0.2 ml) in acetonitrile (2 ml) is added dropwise under stirring. It is left for 2 hours at 0° C., then the temperature is let reach the room temperature. The formed precipitate is filtered and washed with ethyl ether. One gram of product as a solid is obtained.
    • Elementary Analysis
  • Calculated C 46.95% H 3.69% N 10.26%
    Found C 46.99% H 3.72% N 10.22%
  • 1H NMR (200 MHz) (CDCl3): 8.10 (1H, d); 7.9 (1H, t); 7.79 (1H, t); 7.5 (3H, m); 7.30 (1H, d); 5.73 (2H, s); 5.42 (2H, s); 2.20 (3H, s).
    • Example 3 Synthesis of 2-acetyloxybenzoic acid 5-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride of Formula
  • Figure US20090075952A1-20090319-C00025
  • The 2-acetyloxybenzoic acid 5-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride is synthesized according to the process described in Example 1, starting from acetyl salicylic acid and 2,5-bis(chloromethyl)pyridine.
    • A) Synthesis of 2,5-bis(chloromethyl)-pyridine
  • The compound is synthesized according to the process described in Example 1 A) starting from 2,5-pyridin-dimethanol, synthesized in its turn by reduction with NaBH4 of di-ethyl-2,5-pyridin dicarboxylate in ethanol as described in patent JP 48029783.
    • Elementary Analysis
  • Calculated C 50.21% H 3.95% N 7.32% Cl 9.26%
    Found C 50.19% H 3.92% N 7.37% Cl 9.28%
    • Example 4 Synthesis of 2-acetyloxybenzoic acid 3-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride of Formula
  • Figure US20090075952A1-20090319-C00026
  • The 2-acetyloxybenzoic acid 3-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride is synthesized according to the process described in Example 1, starting from acetyl salicylic acid and 2,3-bis(chloromethyl)pyridine.
    • A) Synthesis of 2,3-bis(chloromethyl)-pyridine
  • The compound is synthesized according to the process described in Example 1A) starting from 2,3-pyridin dimethanol, synthesized in its turn by reduction with LiAlH4 of di-methyl-2,3-pyridinedicarboxylate in ethanol as described in J. Chem. Soc., Perkin Trans. 1 (1972), (20), 2485-2490.
    • Elementary Analysis
  • Calculated C 50.21% H 3.95% N 7.32% Cl 9.26%
    Found C 50.25% H 3.93% N 7.30% Cl 9.29%
    • Example 5 Synthesis of 3-nitrooxymethylphenyl ester of the 2-acetoxybenzoic acid
  • Figure US20090075952A1-20090319-C00027
    • A) Preparation of 3-hydroxymethylphenyl Ester of the 2-acetoxybenzoic Acid
  • 3-hydroxymethylphenol (10 g, 0.08 moles) is dissolved in toluene (50 ml) containing triethylamine (9.8 g, 0.1 moles).
  • To the so obtained solution, a solution of the acetylsalicylic acid chloride (16 g, 0.08 moles) in toluene (50 ml) is added under stirring at the temperature of 5-10° C. The mixture is maintained at a temperature within the above range, under stirring for 2 hours, then poured into water and then extracted with dichloromethane (2×100 ml). The organic phase is separated, washed in sequence with a solution of potassium carbonate at 25% w/v, with water, with a 3% hydrochloric acid solution and finally again with water, then anhydrified with sodium sulphate and the solvent evaporated under reduced pressure. The residue is crystallized from isopropanol. 3-hydroxymethyl phenyl ester of the 2-acetoxybenzoic acid (45.8 g, 0.16 moles, yield 80%) is obtained.
  • M.p.: 79-81° C.
  • 1H NMR (CDCl3) δ (ppm): 2.29 (s, 3H); 4.71 (s, 2H); 7.07-8.2 (m, aromatics, 8H).
  • B) Nitration with Fuming Nitric Acid, in the Presence of Sulphuric Acid, of 3-hydroxymethylphenyl Ester of the 2-acetoxybenzoic Acid
  • A solution of fuming nitric acid (3.92 g, 62.2 mmoles, 3 moles with respect to the moles of the hydroxyester under reaction) and sulphuric acid 96% (6.10 g, 62.2 mmoles, 3 moles with respect to the moles of the hydroxyester under reaction) in dichloromethane (25 ml) is cooled to 0° C. and added in one hour time under stirring and under nitrogen atmosphere, with a solution of 3-hydroxymethylphenyl ester of the 2-acetoxybenzoic acid (6 g, 20.7 mmoles) in 25 ml of dichloromethane. The mixture is then diluted with dichloromethane (50 ml) and poured into water and ice (100 g). The organic phase is separated, washed with water, anhydrified with sodium sulphate and the solvent evaporated under reduced pressure. The residue is crystallized from isopropanol obtaining the 3-nitrooxymethylphenyl ester of the 2-acetoxybenzoic acid (5.6 g, 17 mmoles, yield 82%).
  • M.p.: 61-62° C.
  • 1H NMR(CDCl3) δ (ppm): 2.31 (s, 3H); 5.44 (s, 2H); 7.16-8.22 (m, aromatics, 8H).
    • Example 6 Synthesis of 2-(acetyloxy)benzoic acid 4-(nitrooxymethyl)phenyl ester
  • Figure US20090075952A1-20090319-C00028
    • A) Synthesis of 2-(acetoxy)benzoic acid 3-(formyl)phenyl ester
  • To a mixture of 4-hydroxybenzaldeide (20.75 g, 0.17 moles) and triethylamine (0.205 g, 2.4 mmoles) in methylene chloride (300 ml) kept under stirring, under nitrogen inert atmosphere, cooling at a temperature in the range −5° C.-0° C., acetylsalicyloil chloride (41.25 g, 0.21 moles) is added in small aliquots in one hour. After 15 minutes water (250 ml) is added and the phases are separated. The aqueous phase is recovered and separately extracted with methylene chloride. The organic phases are mixed together, they are washed with a 5% carbonate solution (150 ml×2) and then with water (125 ml×2). The organic phase is anhydrified with sodium sulphate in the presence of decolorating carbon. It is filtered under vacuum and the solvent is evaporated under reduced pressure and at a bath temperature lower than 40° C., obtaining 48.2 g of 2-(acetyloxy)benzoic acid 4-(formyl)phenyl ester. The reaction raw product is used without further purification.
    • B) Synthesis of 2-(acetyloxy)benzoic acid 4-(hydroxymethyl)phenyl ester
  • A solution of 2-(acetyloxy)benzoic acid 4-(formyl)phenyl (48.2 g, 0.18 moles) ester in ethyl acetate (500 ml) is hydrogenated in the presence of 5% palladium on carbon (4 g) at room temperature, at hydrogen pressure of about 2.5 atm, under stirring. After 30 minutes the reactor is discharged, the catalyst is removed by filtration under nitrogen atmosphere.
  • The organic phase is washed with a 5% sodium bicarbonate solution and then with water. It is anhydrified with sodium sulphate and the solvent is evaporated at reduced pressure and the residue is used without further purification.
    • C) Synthesis of 2-(acetyloxy)benzoic acid 4-(chloromethyl)phenyl ester
  • To a mixture of 2-(acetyloxy)benzoic acid 4-(hydroxymethyl)phenyl (51.5 g, 0.18 moles) and SOCl2 (153 ml) kept under stirring, dimethylformamide (140 ml) is added at room temperature and it is left under stirring for one hour. At the end the thionyl chloride is evaporated at reduced pressure at a bath temperature lower than 40° C. The thionyl chloride traces in the compound are removed by treating the solid with toluene (60×2), which is then removed by evaporation at reduced pressure at a bath temperature lower than 40° C. The raw product is purified by crystallization with isopropyl ether to give 2-(acetyloxy)benzoic acid 4-(chloromethyl)phenyl ester (32.9 g, 0.10 moles). Yield 60%.
  • 1H NMR: 8.25 (1H, d); 7.68 (1H, t); 7.43 (3H, m); 7.20 (3H, m); 4.60 (2H, s); 2.34 (3H, s).
    • D) Synthesis of 2-(acetyloxy)benzoic acid 4-(nitrooxymethyl)phenyl ester
  • To a solution of 2-(acetyloxy)benzoic acid 4-(chloromethyl)phenyl ester (32.9 g, 0.10 moles) in acetonitrile silver nitrate (22.2 g, 0.12 moles) is added under stirring, sheltered from light. The solution is heated at 70° C. for 4 hours and then cooled to room temperature. The precipitate is filtered and the solvent evaporated at reduced pressure.
  • The residue is purified by chromatography on silica gel eluting with hexane/ethyl acetate (7:3 v/v) to give 2-(acetyloxy)benzoic acid 4-(nitrooxymethyl)phenyl ester (16.6 g, 0.05 moles). M.p. 86-88° C. Yield 50%.
  • 1H NMR (CDCl3): 8.21 (1H, dd); 7.66 (1H, dt); 7.42 (3H, m); 7.20 (3H, m); 5.40 (2H, s), 2.25 (3H, s).
    • Example 7 Synthesis of trans-3-[4-[2-(acetyloxy)benzoyloxy]-3-methoxyphenyl]-2-propenoic acid 4-(nitrooxy)butyl ester
  • Figure US20090075952A1-20090319-C00029
    • A) Synthesis of trans-3-[4-hydroxy-3-methoxyphenyl]-2-propenoic acid 4-bromo butyl ester
  • To a solution of ferulic acid (10 g, 51.5 mmoles) in THF (400 ml) and cooled in a water bath, triphenylphosphine (27.01 g, 103 mmoles) and carbon tetrabromide (34.1 g, 103 mmoles) are in the order added. The mixture is kept under stirring for 5 hours at room temperature. When the reaction is ended, triphenylphosphinoxide is filtered and the solvent is evaporated at reduced pressure. The residue is purified by chromatography on silica gel eluting with hexane/ethyl acetate (7:3 v/v). 7.75 g of trans-3-[4-hydroxy-3-methoxyphenyl]-2-propenoic acid 4-bromobutyl ester as a white solid are obtained. M.p. 86-89° C. Yield 46%.
    • Synthesis of trans-3-[4-[2-(acetyloxy)benzoyloxy]-3-methoxyphenyl]-2-propenoic acid 4-bromo butyl ester
  • To a solution of trans 3-[4-hydroxy-3-methoxyphenyl]-2-propenoic acid 4-bromo butyl ester (2 g, 6.1 mmoles) in CHCl3 (20 ml) an acetylsalicylic acid mixture (1.1 g, 6.1 mmoles) in DMF (2 ml) is added and it is cooled to 0° C., then DCC (1.50 g, 7.2 mmoles) and DMAP (74 mg, 6×10−3 mmoles) are added. It is left at the same temperature for 30 minutes and at room temperature for 16 hours. The precipitate is filtered and the solvent is evaporated at reduced pressure. The residue is dissolved in ethyl acetate (100 ml×2 times) and washed with water and NaCl. The organic phase is anhydrified and the solvent is evaporated at reduce pressure.
  • The residue is purified by chromatography on silica gel eluting with hexane/ethyl acetate (8:2 v/v) to give the trans-3-[4-[2-(acetyloxy)benzoyloxy]-3-methoxyphenyl]-2-propenoic acid 4-bromo butyl ester (1.1 g, Yield 37%).
  • 1H NMR CDCl3: 8.25 (1H, d); 7.65 (2H, m); 7.40 (1H, t); 7.20 (4H, m); 6.39 (1H, d); 4.25 (2H, t); 3.85 (3H, s); 3.47 (2H, t); 2.29 (3H, s); 2.01 (2H, m); 1.89 (2H, m).
    • Example 8 Synthesis of trans-3-[4-(4′-nitrooxybutyryloxy)-3-methoxyphenyl]-2-propenoic acid 4-(acetylamino)phenyl ester
  • Figure US20090075952A1-20090319-C00030
    • A) Synthesis of trans-3-[4-acetyloxy-3-methoxyphenyl]-2-propenoic acid
  • To a solution of ferulic acid (5 g, 25.75 mmoles) in pyridine (75 ml) cooled to 0° C. and sheltered from light, acetic anhydride (13.14 g, 128.7 mmoles) is added in small aliquots. When the addition is ended the temperature is let reach the room value maintaining the solution under magnetic stirring for 24 hours. HCl 18.5% (160 ml) is added up to pH 2, one extracts with ethyl acetate and the organic phase is anhydrified and the solvent is evaporated at a reduced pressure. 5.15 g of trans-3-[4-acetyloxy-3-methoxyphenyl]-2-propenoic acid are obtained as a white solid. M.p. 199-205° C. Yield 85%.
    • B) Synthesis of trans-3-3-[4-acetyloxy-3-methoxyphenyl]-2-propenoyl chloride
  • To a suspension of trans-3-[4-acetyloxy-3-methoxyphenyl]-2-propenoic acid (4 g, 16.93 mmoles) in toluene (70 ml) and dimethylformamide (10 ml) cooled in an ice bath, oxalyl chloride (4.30 g, 33.87 mmoles) is dropped. The mixture is maintained under stirring at 0° C. for 1 hour then the temperature is let reach the room value and it is left for 2 hours. The solvent is removed at reduced pressure and the raw product is used without further purification.
    • C) Synthesis of trans-3-[4-acetyloxy-3-methoxyphenyl]-2-propenoic acid 4-(acetylamino)phenyl ester
  • To a solution of paracetamol (2.56 g, 16.92 mmoles) in pyridine (20 ml) cooled in an ice bath trans-3-[4-acetyloxy-3-methoxyphenyl]-2-propenoyl chloride (4.31 g, 16.92 mmoles) dissolved in acetone (45 ml) is dropped. The mixture is maintained under stirring in ice for 3 hours then it is poured into water (300 ml) and the precipitate is filtered and triturated with hexane to give trans-3-[4-acetyloxy-3-methoxyphenyl]-2-propenoic acid 4-(acetylamino)phenyl ester (4.38 g) as an orange solid. M.p. 246-250° C. Yield 70%.
    • D) Synthesis of trans-3-[4-hydroxy-3-methoxyphenyl]-2-propenoic acid 4-(acetylamino)phenyl ester
  • To a solution of trans-3-[4-acetyloxy-3-methoxyphenyl]-2-propenoic acid 4-(acetylamino)phenyl ester (4.2 g, 11.37 mmoles) in methanol (650 ml) and tetrahydrofuran (850 ml), potassium carbonate (9.11 g, 65.95 mmoles) dissolved in water (50 ml) is added and it is left under stirring at room temperature for 2 hours. The precipitate is filtered and the solution is brought to pH 6 with HCl 5% (15 ml). One extracts with ethyl acetate and the organic phase is anhydrified and removed from the solvent at reduced pressure. The raw product is purified by chromatography on silica gel eluting with chloroform/methanol (9/0.5 v/v). Trans-3-[4-hydroxy-3-methoxyphenyl]-2-propenoic acid 4-(acetylamino)phenyl ester (2.1 g) is obtained as a white solid. M.p. 185-195° C. Yield 56%.
  • 1H NMR (CDCl3): 10 (1H, s); 9.8 (1H, s); 7.8 (1H, d); 7.7 (2H, d); 7.3 (2H, d); 7.1 (2H, d); 6.9 (1H, d); 6.7 (1H, d); 3.8 (3H, s); 2 (3H, s).
    • E) Synthesis of trans-3-[4-(4′-bromobutyryloxy)-3-methoxyphenyl]-2-propenoic acid 4-(acetylamino)phenyl ester
  • To a solution of trans-3-[4-hydroxy-3-methoxyphenyl]-2-propenoic acid 4-(acetylamino)phenyl ester (1.6 g, 4.8 mmoles) in pyridine (12 ml) cooled in ice bath, 4-bromobutyryl chloride (1.3 g, 7.2 mmoles) dissolved in acetone (15 ml) is dropped and it is maintained under stirring for 7 hours. It is poured into water and ice, the precipitate is filtered and treated with hexane. Trans-3-[4-(4′bromobutyryloxy)-3-methoxyphenyl]-2-propenoic acid 4-(ace-tyl amino)phenyl ester (1.8 g) is obtained. Yield 67%.
    • F) Synthesis of trans-3-[4-(4′-nitrooxybutyryloxy)-3-methoxyphenyl]-2-propenoic acid 4-(acetylamino)phenyl ester
  • To a solution of trans-3-[4-(4′bromobutyryloxy)-3-methoxyphenyl]-2-propenoic acid 4-(acetylamino)phenyl ester (1.8 g, 3.78 mmoles) in acetonitrile (100 ml), silver nitrate (1.28 g, 7.56 mmoles) is added sheltered from light. It is left at 80° C. for 13 hours then the precipitate is filtered. The raw product is purified by chromatography on silica gel eluting with hexane/ethyl acetate (3/7 v/v). Trans-3-[4-(4/nitrooxy-butyryloxy)-3-methoxyphenyl]-2-propenoic acid 4-(acetylami-no)phenyl ester is obtained.
  • 1H NMR (CDCl3): 7.8 (1H, d); 7.5 (3H, m); 7.1 (5H, m); 6.5 (1H, d); 4.6 (2H, t); 3.8 (3H, s); 2.7 (2H, t); 2.17 (5H, m).
    • Example 9 Synthesis of 4-nitrooxybutanoic acid 4′-acetylamino phenyl ester
  • Figure US20090075952A1-20090319-C00031
    • A) Preparation of 4-bromobutanoic acid 4′-acetylamino phenyl ester
  • To a solution of 4-bromobutyric acid (4.6 g, 27.6 mmoles) in chloroform (45 ml) and N,N-dimethylformamide (20 ml), paracetamol (4.17 g, 27.6 mmoles), N,N′-dicyclohexyl carbodiimide (8.42 g, 40.8 mmoles) and 4-dimethyl aminopyridine (0.15 g, 1.25 mmoles) are added. The reaction mixture is kept under stirring at room temperature for 72 hours, filtered and evaporated under vacuum. The reaction raw product is treated with ethyl acetate and washed with brine and then with water. The organic phase is anhydrified with sodium sulphate and then evaporated under vacuum.
  • The residue is purified by chromatography on silica gel eluting with n-hexane/ethyl acetate 4/6 (v/v ratio). 5.33 g of the product as a white solid are obtained.
  • M.p.=108-110° C.
    • B) Preparation of 4-nitrooxybutanoic acid 4′-acetylamino phenyl ester
  • To a solution of 4-bromobutanoic acid 4′-acetylamino phenyl ester (5.33 g, 17.8 mmoles) in acetonitrile (80 ml) silver nitrate (4.56 g, 26.9 mmoles) is added. The reaction mixture is heated for 16 hours in absence of light at 80° C., then cooled to room temperature, filtered for removing the silver salts, and evaporated at reduced pressure. The residue is purified by chromatography on silica gel, eluting with n-hexane/ethyl acetate 4/6. 4.1 g of the product as a white solid are obtained.
  • M.p.=80-83° C.
  • Elementary analysis: C H N
    calc. 51.07% 4.99% 9.92%
    found 51.06% 5.00% 9.90%
  • 1H NMR (CDCl3): 7.55 (1H, s); 7.49 (2H, d); 7.02 (2H, d); 4.58 (2H, t); 2.71 (2H, t); 2.19 (2H, m); 2.14 (3H, s).
    • Example 10 Synthesis of 4-(nitrooxymethyl)-benzoic acid 4-acetylamino phenyl ester
  • Figure US20090075952A1-20090319-C00032
    • A) Preparation of 4-(chloromethyl)-benzoic acid 4-acetylamino phenyl ester
  • To a solution of paracetamol (2 g, 13.23 mmoles) in tetrahydrofuran (80 ml), triethylamine (1.34 g, 13.23 mmoles) and 4-(chloromethyl)-benzoylchloride (2.5 g, 13.23 mmoles) are added. The reaction mixture is kept under stirring at room temperature for 24 hours, then the solvent is evaporated at reduced pressure and the reaction raw product is purified by chromatography on silica gel, eluting with methylene chloride/methanol 20/0.5 (v/v ratio) to give 2.6 g of 4-(chloromethyl)-benzoic acid 4-acetylamino phenyl ester. (Yield 65%)
  • 1H NMR (CDCl3): 8.1 (2H, d); 7.69 (2H, d); 7.45 (2H, d); 7.02 (2H, d); 4.9 (2H, s); 2.14 (3H, s).
    • B) Preparation of 4-(nitrooxymethyl)-benzoic acid 4-acetylamino phenyl ester
  • To a solution of 4-(chloromethyl)-benzoic acid 4-acetylamino phenyl ester (2 g, 6.6 mmoles) in acetonitrile (80 ml) silver nitrate (2.24 g, 13.18 mmoles) is added. The reaction mixture is heated for 20 hours in absence of light at 60° C., then cooled to room temperature, filtered for removing the silver salts, and evaporated at reduced pressure. The residue is purified by chromatography on silica gel, eluting with n-hexane/ethyl acetate 3/7 (v/v ratio). 1.13 g of 4-(nitrooxymethyl)-benzoic acid 4-acetylamino phenyl ester are obtained. (Yield 52%)
  • 1H NMR (CDCl3): 8.1 (2H, d); 7.69 (2H, d); 7.45 (2H, d); 7.02 (2H, d); 5.74 (2H, s); 2.14 (3H, s).
    • Example 11 Preparation of 3-(nitrooxymethyl)-benzoic acid 4-acetylamino phenyl ester
  • Figure US20090075952A1-20090319-C00033
    • A) Preparation of 3-(chloromethyl)-benzoic acid 4-acetylamino phenyl ester
  • To a solution of paracetamol (2 g, 13.23 mmoles) in tetrahydrofuran (80 ml), triethylamine (1.34 g, 13.23 mmoles) and 4-(chloromethyl)-benzoylchloride (2.5 g, 13.23 mmoles) are added. The reaction mixture is kept under stirring at room temperature for 24 hours, then the solvent is evaporated at reduced pressure and the reaction raw product is purified by chromatography on silica gel, eluting with methylene chloride/methanol 20/0.5-(v/v ratio) to give 2.9 g of 3-(chloromethyl)-benzoic acid 4-acetylamino phenyl ester. (Yield 73%)
  • 1H NMR (CDCl3): 8.1 (1H, s); 8.02 (1H, d); 7.77 (1H, d); 7.65 (1H, m); 7.45 (2H, d); 7.02 (2H, d); 4.9 (2H, s); 2.14 (3H, s).
    • B) Preparation of 3-(nitrooxymethyl)-benzoic acid 4-acetylamino phenyl ester
  • To a solution of 3-(chloromethyl)-benzoic acid 4-acetylamino phenyl ester (2.5 g, 8.2 mmoles) in acetonitrile (80 ml) silver nitrate (2.8 g, 16.4 mmoles) is added. The reaction mixture is heated for 20 hours in absence of light at 60° C., then cooled to room temperature, filtered for removing the silver salts, and evaporated at reduced pressure. The residue is purified by chromatography on silica gel, eluting with n-hexane/ethyl acetate 3/7 (v/v ratio). 1.5 g of 3-(nitrooxymethyl)-benzoic acid 4-acetylamino phenyl ester are obtained. (Yield 55%)
  • 1H NMR (CDCl3): 8.1 (1H, s); 8.02 (1H, d); 7.77 (1H, d); 7.65 (1H, m); 7.45 (2H, d); 7.02 (2H, d); 5.74 (2H, s); 2.14 (3H, s).
    • Example 12 Synthesis of 2-(nitrooxymethyl)-benzoic acid 4-acetylamino phenyl ester
  • Figure US20090075952A1-20090319-C00034
    • A) Preparation of 2-(chloromethyl)-benzoylchloride
  • To thionyl chloride (35 ml) cooled at 0° C. with ice bath, the 2-hydroxymethylbenzoic acid (4 g, 26.3 mmoles) is added. The temperature is let reach the room value and the mixture is left under stirring for 2 hours, then it is evaporated at reduced pressure and treated 3 times with chloroform for completely removing the thionyl chloride. The reaction raw product is used without further purification.
    • B) Preparation of 2-(chloromethyl)-benzoic acid 4-acetyl amino phenyl ester
  • To a solution of paracetamol (2 g, 13.23 mmoles) in tetrahydrofuran (80 ml), triethylamine (1.34 g, 13.23 mmoles) and 2-(chloromethyl)-benzoylchloride (2.5 g, 13.23 mmoles) are added. The reaction mixture is kept under stirring at room temperature for 24 hours, then the solvent is evaporated at reduced pressure and the reaction raw product is purified by chromatography on silica gel, eluting with methylene chloride/methanol 20/0.5 (v/v ratio) to give 1.9 g of 2-(chloromethyl)-benzoic acid 4-acetylamino phenyl ester. (Yield 47%)
  • 1H NMR (CDCl3): 8.22 (1H, d); 7.41 (5H, m); 7.02 (2H, d); 4.9 (2H, s); 2.14 (3H, s).
    • C) Preparation of 2-(nitrooxymethyl)-benzoic acid 4-acetylamino phenyl ester
  • To a solution of 2-(chloromethyl)-benzoic acid 4-acetylamino phenyl ester (1.5 g, 4.9 mmoles) in acetonitrile (80 ml) silver nitrate (1.68 g, 9.8 mmoles) is added. The reaction mixture is heated for 20 hours in absence of light at 60° C., then cooled to room temperature, filtered for removing the silver salts, and evaporated at reduced pressure. The residue is purified by chromatography on silica gel, eluting with n-hexane/ethyl acetate 3/7 (v/v ratio). 0.77 g of 2-(nitrooxymethyl)-benzoic acid 4-acetylamino phenyl ester are obtained. (Yield 48%)
  • 1H NMR (CDCl3): 8.22 (1H, d); 7.41 (5H, m); 7.02 (2H, d); 5.40 (2H, s); 2.14 (3H, s).
    • Example 13 Synthesis of 2-acetylamino-3-(4-nitrooxybutyryl)-3-mercaptopropionic acid 4-acetylamino phenyl ester
  • Figure US20090075952A1-20090319-C00035
    • A) Preparation of 2-acetylamino-3-(4-bromobutyryl)-3-mercaptopropionic acid
  • To a solution of 4-bromobutyric acid (3 g, 17.9 mmoles) in 35 ml of chloroform, carbonyl-diimidazole (2.9 g, 17.9 mmoles) is added and it is left under stirring at room temperature for one hour. Then N-acetylcisteine (2.9 g, 17.9 mmoles), sodium ethylate (40 mg, 0.58 mmoles) and dimethylformamide (5 ml) are added and the mixture is left under stirring at room temperature for 15 hours. Diluted HCl is added and the organic phase is separated. The aqueous phase brought to pH 3-3.5 is extracted with ethyl acetate. The organic phases mixed together are anhydrified with sodium sulphate and evaporated at reduced pressure. The reaction raw product is purified by chromatography on silica gel, eluting with chloroform/ethyl acetate 3/7 (v/v ratio). 2.06 g of 2-acetylamino-3-(4-bromobutyryl)-3-mercapto-propionic acid are obtained. (Yield 37%)
  • 1H NMR (CDCl3): 10.0 (1H, s); 6.89 (1H, d); 4.78 (1H, m); 3.40 (4H, m); 2.77 (2H, t); 2.18 (2H, m); 2.04 (3H, s).
    • B) Preparation of 2-acetylamino-3-(4-bromobutyryl)-3-mercaptopropionic acid 4-acetylamino phenyl ester
  • To a solution of 2-acetylamino-3-(4-bromobutyryl)-3-mercaptopropionic acid in chloroform (20 ml) and dimethylformamide (20 ml), cooled at 0° C. with ice bath, paracetamol (1 g, 7.2 mmoles), dicyclohexylcarbodiimide (1.17 g, 5.6 mmoles) and N,N-dimethyl aminopyridine (90 mg) are added. The temperature is let reach the room value and the mixture is left under stirring for 24 hours. The precipitate is filtered and the organic phase is washed with water. The organic phase is anhydrified with sodium sulphate and the solvent is evaporated at reduced pressure. The raw product has been purified by chromatography on silica gel eluting with methylene chloride/methanol 20/0.5 (v/v ratio). 0.6 g of 2-acetylamino-3-(4-bromobutyryl)-3-mercaptopropionic acid 4-acetylamino phenyl ester are obtained. (Yield 32%)
  • 1H NM (CDCl3): 7.45 (2H, d); 7.00 (2H, m); 4.80 (1H, m); 3.52 (2H, t); 3.32 (2H, d); 2.7 (2H, t); 2.1 (2H, m); 2.00 (3H, s).
    • C) Preparation of 2-acetylamino-3-(4-nitrooxy butyryl)-3-mercaptopropionic acid 4-acetylamino phenyl ester
  • To a solution of 2-acetylamino-(4-bromobutyryl)-3-mercaptopropionic acid 4-acetylamino phenyl ester (0.5 g, 1.26 mmoles) in acetonitrile (40 ml) silver nitrate (0.43 g, 2.52 mmoles) is added. The reaction mixture is heated for 20 hours in absence of light at 80° C., then cooled to room temperature, filtered for removing the silver salts, and evaporated at reduced pressure. The residue is purified by chromatography on silica gel, eluting with n-hexane/ethyl acetate 3/7 (v/v ratio). 0.31 g of 2-acetylamino-3-(4-nitrooxybutyryl)-3-mercaptopropionic acid 4-acetylamino phenyl ester are obtained. (Yield 63%)
  • 1H NMR (CDCl3): 7.45 (2H, d); 7.00 (2H, m); 4.80 (1H, m); 4.57 (2H, t); 3.32 (2H, d); 2.7 (2H, t); 2.1 (2H, m); 2.00 (3H, s).
    • Example 14 Synthesis of 3-[(2-nitrooxy)ethyloxy]propanoic acid 4-acetylamino phenyl ester
  • Figure US20090075952A1-20090319-C00036
    • A) Preparation of 3-[(2-hydroxy)ethyloxy]propanoic-acid-4-acetylamino phenyl ester
  • To a solution of paracetamol (5 g, 33.6 mmoles) in chloroform (80 ml) and dimethylformamide (80 ml), cooled at 0° C. with ice bath, 3-[(2-hydroxy)ethyloxy]propanoic acid (3 g, 22.38 mmoles), dicyclohexylcarbodiimide (6.9 g, 33.6 mmoles) and dimethylaminopyridine (0.2 g, 1.68 mmoles) are added. The temperature is let reach the room value and the mixture is left under stirring for 24 hours. The precipitate is filtered and the organic phase is washed with water and extracted with chloroform. The organic phase is anhydrified with sodium sulphate and the solvent evaporated at reduced pressure. The raw product is purified by chromatography on silica gel eluting with methylene chloride/methanol 20/0.5 (v/v ratio). 1.3 g of 3-[(2-hydroxy)ethyloxy]propanoic acid 4-acetylamino phenyl ester are obtained. (Yield 33%)
  • 1H NMR (CDCl3): 7.45 (2H, d); 7.02 (2H, d); 4.40 (2H, t); 3.75 (6H, m); 2.14 (3H, s).
    • B) Preparation of 3-[(2-iodo)ethyloxy]propanoic acid 4-acetylamino phenyl ester
  • To a solution of 3-[(2-hydroxy)ethyloxy]propanoic acid 4-acetylamino phenyl ester (1.5 g, 5.6 mmoles), imidazol (0.57 g, 8.4 mmoles) and triphenylphosphine (1.9 g, 7.28 mmoles) in ether (15 ml) and acetonitrile (10 ml) cooled at 0° C. with ice bath, iodine (1.99 g, 7.84 mmoles) is added and it is left under stirring at 0° C. for 2 hours. Then the temperature is let reach the room value, hexane is added, the precipitate is filtered and the solvent is evaporated at reduced pressure. The raw product is purified by chromato-graphy on silica gel eluting with hexane/ethyl acetate 3/7 (v/v ratio). 1 g of 3-[(2-iodo)ethyloxy]propanoic acid 4-acetylamino phenyl ester is obtained.
  • (Yield 48%)
  • 1H NMR (CDCl3): 7.45 (2H, d); 7.02 (2H, d); 4.40 (2H, t); 3.75 (4H, t); 3.54 (2H, t); 2.14 (3H, s).
    • C) Preparation of 3-[(2-nitrooxy)ethyloxy]propanoic acid 4-acetylamino phenyl ester
  • To a solution of 3-[(2-iodo)ethyloxy]propanoic acid 4-acetylamino phenyl ester (1 g, 2.64 mmoles) in acetonitrile (40 ml) silver nitrate (0.9 g, 5.28 mmoles) is added. The reaction mixture is heated for 5 hours in absence of light at 60° C., then cooled to room temperature, filtered for removing the silver salts, and evaporated at reduced pressure. The residue is purified by chromatography on silica gel, eluting with n-hexane/ethyl acetate 3/7 (v/v ratio). 0.46 g of 3-[(2-nitrooxy)ethyloxy]propanoic acid 4-acetylamino phenyl ester are obtained. (Yield 56%)
  • 1H NMR (CDCl3): 7.45 (2H, d); 7.02 (2H, d); 4.58 (2H, t); 4.40 (2H, t); 3.75 (4H, t); 2.14 (3H, s).
    • Example 15 Synthesis of 2-hydroxybenzoic acid 3-(nitrooxymethyl)phenyl ester
  • Figure US20090075952A1-20090319-C00037
  • To a solution of 2-(acetyloxy)benzoic acid 3-(nitroxymethyl)phenylester (2 g, 6.04 mmoles), obtained as described in Example 5, in tetrahydrofuran (10 ml), methanol (5 ml) and water (4 ml), imidazol (0.04 g, 0.6 mmoles) is added. The mixture is left under stirring at room temperature for 20 days, then the solvent is evaporated at reduced pressure, the residue is treated with ethyl acetate and washed with water.
  • The organic phase is anhydrified with sodium sulphate and the solvent is evaporated at reduced pressure. The reaction raw product is purified by chromatography on silica gel using as eluent hexane/ethyl acete (9/1 v/v) to give 2-hydroxybenzoic acid 3-(nitrooxmethyl)phenylester (0.8 g). Yield 46%.
  • 1H NMR (CDCl3): 10.46 (1H, s); 8.13 (1H, dd); 7.56 (2H, m); 7.34 (3H, m); 7.05 (2H, m); 5.51 (2H, s).
    • Example 16 Synthesis of trans-3-[4-[α-methyl-[4-(-2-methylpropyl)benzene]acetyloxy]-3-methoxyphenyl]-2-propenoyl 4-(nitrooxy)butyl ester Having Formula
  • Figure US20090075952A1-20090319-C00038
    • A) Synthesis of trans-3-[4-[α-methyl-[4-(-2-methylpropyl)benzene]acetyloxy]-3-methoxyphenyl]-2-propenoic acid
  • To a solution of α-methyl-[4-(2-methylpropyl)benzene]acetic acid (5.03 g, 24.4 mmoles) in tetrahydrofuran (100 ml) and N,N-dimethylformamide (5 ml), 1,1-carbonyldiimidazol (4.25 g, 24.8 mmoles) is added. After 1 hour the obtained solution is treated with ferulic acid (4.90 g, 25 mmoles), sodium ethylate (89 mg) is added and it is left at room temperature under stirring for, 12 hours. The reaction mixture is washed with HCl 5%, then with water and at last with brine. The organic phase is anhydrified with sodium sulphate and evaporated at reduced pressure.
  • The obtained residue is purified by chromatography on silica gel, eluting with ethyl acetate/n-hexane 7/3. 5.1 g of trans-3-[4-[α-methyl-[4-(-2-methylpropyl)benzene]acetyl]-3-methoxyphenyl]-2-propenoic acid are obtained as a with solid having m.p. 131-137° C.
  • 1H-NMR (CDCl3): 7.7.2 (1H, d), 7.32 (2H, dd), 7.26 (1H, m), 7.16-7.07 (4H, m), 6.98 (1H, d), 6.37 (1H, d), 3.99 (1H, q), 3.73 (3H, s), 2.47 (2H, d), 1.88 (1H, m), 1.63 (3H, d), 0.92 (6H, d).
    • B) Synthesis of trans-3-[4-[α-methyl-[4-(-2-methylpropyl)benzene]acetyloxy]-3-methoxyphenyl]-2-propenoyl 4-bromobutyl ester
  • To a solution of trans-3-[4-[α-methyl-[4-(2-methylpropyl)benzene]acetyloxy]-3-methoxyphenyl]-2-propenoic acid (5.33 g, 14 mmoles) in N,N-dimethylformamide (130 ml), sodium ethylate (1.2 g, 16 mmoles) is added under stirring. After 1 hour to the obtained mixture 1,4-dibromobutane (10 g, 46 mmoles) is added and the mixture is let react at room temperature for 12 hours. The reaction mixture is washed with 5% HCl, then with water and at last with brine, the organic phase is anhydrified with sodium sulphate and evaporated at reduced pressure. The obtained residue is purified by chromatography on silica gel eluting with n-hexane/ethyl acetate 8/2. 4.46 g of trans-3-[4-hydroxy-[α-methyl-[4-(-2-methylpropyl)benzene]acetyl]-3-methoxyphenyl]-2-propenoyl 4-bromobutyl ester are obtained.
    • C) Synthesis of trans-3-[4-[α-methyl-[4-(-2-methylpropyl)benzene]acetyloxy]-3-methoxyphenyl]-2-propenoyl 4-(nitrooxy)butyl ester
  • To a solution of trans-3-[4-[α-methyl-[4-(-2-methylpropyl)benzene]acetyloxy]-3-methoxyphenyl]-2-propenoyl 4-bromobutyl ester (4 g, 7.72 mmoles) in acetonitrile (70 ml) silver nitrate (2.58 g, 15 mmoles) is added. The reaction mixture is heated under reflux for 2 hours sheltered from light. At the end the formed salt is removed by filtration and the solution is evaporated at reduced pressure. The recovered residue is purified by chromatography on silica gel, eluting with n-hexane/ethyl acetate 8/2. 2.4 g of trans-3-[4-[α-methyl-[4-(-2-methylpropyl)benzene]acetyloxy]-3-methoxyphe-nyl]-2-propenoyl 4-(nitrooxy)butyl ester are obtained as oil.
  • 1H-NMR (CDCl3): 7.62 (1H, d), 7.32 (2H, d), 7.15 (2H, d), 7.16-7.05 (2H, m), 6.96 (1H, d), 6.35 (1H, d), 4.51 (2H, t), 4.24 (2H, t), 3.99 (1H, q), 3.74 (3H, s), 2.43 (2H, d), 1.89-1.83 (5H, m), 1.62 (3H, d), 0.92 (6H, d).
    • Elementary Analysis:
  • Calculated C: 64.91% H: 6.66% N: 2.82%
    Found C: 64.83% H: 6.52% N: 2.69%
    • Example 17 Synthesis of trans-3-[4-[2-fluoro-α-methyl-(1,1′-biphenyl)-4-acetyloxy]-3-methoxyphenyl]-2-propenoyl 4-(nitrooxy)butyl ester Having Formula
  • Figure US20090075952A1-20090319-C00039
  • The compound is synthesized according to the process described in Example 16. The process total yield is 32%. The substance appears as an amorphous solid.
  • 1H-NMR (CDCl3): 7.40-7.25 (9H, m), 7.07-7.01 (2H, d), 6.98 (1H, m), 6.38 (1H, d), 4.44 (2H, t), 4.46 (2H, t), 4.21 (2H, t), 4.04 (1H, q), 3.73 (3H, s), 1.72 (4H, m), 1.65 (3H, d).
    • Elementary Analysis:
  • Calculated C: 64.79% H: 5.25% N: 2.62% F: 3.53%
    Found C: 64.85% H: 5.31% N: 2.74% F: 3.48%
    • Example 18 Synthesis of (Z)-5-fluoro-2-methyl-1-[[4-(methylsulphinyl phenyl]methylene]-1H-indene-3-acetic acid (4-nitrooxy)butyl ester
  • Figure US20090075952A1-20090319-C00040
    • A) Synthesis of (Z)-5-fluoro-2-methyl-1-[[4-(methylsulphinyl)phenyl]methylene]-1H-indene-3-àcetic acid 4-bromobutyl ester
  • To a solution of Sulindac (5.17 g, 14.5 mmoles) in dimethylformamide (50 ml) EtONa (1.18 g, 16.4 mmoles) is added. The reaction mixture is kept under stirring for one hour, then 1,4-dibromobutane dissolved in dimethylformamide (20 ml) is added.
  • The reaction mixture is kept under stirring at room temperature for 8 hours, ethyl acetate is added and the mixture is washed with water. The organic phase is anhydrified with sodium sulphate and the solvent is evaporated at reduced pressure.
  • The reaction raw product is purified by chromatography on silica gel eluting with a mixture of hexane/ethyl acetate (3/7 v/v). Cis-5-fluoro-2-methyl-1-[[4-(methylsulphinyl)phenyl]methylene]-1H-indene-3-acetic acid 4-bromobutyl ester (3.8 g) is obtained as a yellow solid. Yield 55%.
    • B) Synthesis of (Z)-5-fluoro-2-methyl-1-[[4-(methylsulphinyl)phenyl]methylene]-1H-indene-3-acetic acid (4-nitrooxy)butyl ester
  • To a solution of cis-5-fluoro-2-methyl-1-[[4-(methyl sulphinyl)phenyl]methylene]-1H-indene-3-acetic acid 4-bromobutyl ester (3.8 g, 7.7 mmoles) in acetonitrile (50 ml) AgNO3 (3.9 g, 22.3=moles) is added sheltered from light. The mixture is heated at 80° C. for 48 hours, then the precipitate is filtered and the solvent is evaporated. The reaction raw product is purified by chromatography on silica gel eluting with a mixture of hexane/ethyl acetate (1/9 v/v). (Z)-5-fluoro-2-methyl-1-[[4-(methylsulphinyl)phenyl]methylene]-1H-indene-3-acetic acid (4-nitrooxy)butyl ester (2.6 g) is obtained as a yellow solid. Yield 68%.
  • 1H NMR (CDCl3): 7.78-7.62 (4H, m); 7.17 (2H, m); 6.88 (1H, dd); 6.60-6.50 (1H, m); 4.39 (2H, t); 4.16 (2H, t); 3.57 (2H, s); 2.79 (3H, s); 2.20 (3H, s); 1.79-1.61 (4H, m).
    • Example 19 Synthesis of (Z)-5-fluoro-2-methyl-1-[[4-(methylsulphinyl)phenyl]methylene]-1H-indene-3-acetic acid 6(nitrooxymethyl)-2-methyl pyridinyl ester
  • Figure US20090075952A1-20090319-C00041
  • The (Z)-5-fluoro-2-methyl-1-[[4-(methylsulphinyl)phenyl]methylene]-1H-indene-3-acetic acid 6(nitrooxymethyl)-2-methyl pyridinyl ester is synthesized according to the process described in Example 1, starting from (Z)-5-fluoro-2-methyl-1-[[4-(methylsulphinyl)phenyl]methylene]-1H-indene-3-acetic acid and 2,6-bis(chloromethyl)pyridine. Total yield of the process 20%.
    • Elementary Analysis:
  • Calculated C 57.09% N 5.12% F 3.47% Cl 6.48% S 5.86%
    Found C 57.19% N 4.51% F 3.43% Cl 6.51% S 5.84%
    • Example 20 Synthesis of 2-acetyloxybenzoic acid 2-(nitrooxymethyl)phenyl ester
  • Figure US20090075952A1-20090319-C00042
  • The 2-acetyloxybenzoic acid 2-(nitrooxymethyl)phenyl ester is synthesized according to the process described in Example 6, starting from acetylsalicylic acid and 2-hydroxybenzaldehyde. Total yield of the process 68%.
  • 1H NMR (CDCl3): 8.22 (1H, dd); 7.68 (1H, dt); 7.35 (6H, m); 5.40 (2H, s); 2.30 (3H, s).
    • Pharmacological Examples Example F1 Determination of the Capability of the Compounds of the Invention to Protect the Animals from the Liver Injury Induced by Concanavalin A
  • The model in vivo used in the present example has been described in Tiegs G. Hentshel J, A Wendel. A T cell-dependent experimental liver injury in mice induced by Concanavalin A. J. Clin. Invest. 1992; 90:196-203.
  • The animals (rats of Swiss stock weighing about 20 g) are divided in groups of at least No. 10 animals for group.
  • The animals receive concanavalin and solvent (treated control group), solvent (polyethylene glycol 400—untreated control group), concanavalin and tested compound dissolved in the solvent (treated groups).
  • Rats are treated intravenously with concanavalin A (0.3 mg/rat), and after 5 minutes they receive by intraperitoneal injection the tested compounds, at the doses reported in Table 1, dissolved in polyethyleneglycol 400.
  • Eight hours after the concanavalin A injection all the animals were sacrificed and the blood collected and examined. The data, reported in Table 1, are expressed as value of the plasmatic glutamic-pyruvic transaminase percentage of the animals treated with the tested compound with respect to the animals of the treated control group.
  • The results show that the compounds according to the invention protect from the liver injury induced by concanavalin A, while the native or precursor compounds even worsen the liver injury.
    • Example F2 Determination of the Antiproliferative Activity of the Compounds of the Invention in Cancerous Cells
  • Human adenocarcinoma (HT29) cells taken from colon affected by cancerous process were transferred into plates with 24 wells containing a cellular culture medium formed by 10% of foetal bovine serum, penicillin (50. U/ml), streptomycin (50 mg/ml) and PEG 400 (polyethyleneglycol). After 24 hours a part of the plates is inoculated with the tested compounds dissolved in the carrier (PEG 400). 96 hours after the inoculation of the compounds the cellular growth was measured by haemocytometer. The results, reported in Table 2, are expressed as percentage of the cellular proliferation with respect to the controls.
  • The obtained results show that the compounds of the invention are much more effective in inhibiting the proliferation of the cancerous cells with respect to the corresponding native compounds.
    • Example F3 Determination of the Antiproliferative Activity of the Compounds of the Invention in Cancerous Epithelial Cells of Bladder and Prostate
  • The experiment was carried out by using three human epithelial cellular lines of the prostate cancer (PNT1A; LLNCaP; PC3) and three human epithelial cellular lines of the bladder cancer (T24; 647V; 1207), the various types of cellular lines are identified on the basis of the characteristics, in particular of the aggressiveness, of the cancerous process.
  • The cancerous cells are sown, with an initial concentration of 20,000 cells/cm2, in plates having 96 wells with a cellular culture medium RPMI added with foetal bovine serum 5% and L-Glutamine 1%. Solutions in dimethylsulphoxide of the tested compounds at three different concentrations (10−6 M; 10−5 M; 10−4M) or the carrier (DMSO 10/00) are added to the culture medium. 4 days after the treatment the cellular growth was measured by the method with MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) de-scribed by Turner in: Turner T., Chen P., Goodly L. J., Wells A. Clin. Exp. Metastasis 1996, 14, 409-418. The results, reported in Table 3, are expressed as inhibition percentage of the cellular proliferation determined by measuring the cellular proliferation in the cellular cultures treated with the tested compounds with respect to that measured in cellular cultures treated with dimethylsulphoxide 10/00.
  • The results reported in Table 3 show that the nitrooxybutyl ester of sulindac (Ex. 18) at the 10−5 M concentration has a strong inhibitory effect on the proliferation of all kinds of cancerous cells examined; the compound of Ex. 16, reported in the Table with the simplified denomination of nitrooxybutyl ester of the ibuprofen der. with ferulic ac. and the compound of Ex. 17, reported in the Table with the simplified denomination of nitrooxybutyl ester of the flurbiprofen der. with ferulic ac., are active in very aggressive prostate and bladder tumours, as it is shown by the results obtained on the cellular lines LNCaP and PC3, and 647V, 1207. The compound of Ex. 7, reported in the Table with the simplified denomination of nitrooxybutyl ester of the aspirin der. with ferulic ac. is active, at 10−5 concentrations, in prostate tumours as shown by the results obtained on the cellular lines LNCaP and PC3.
    • Example F4 Determination In Vitro of the Effect of the Compounds of the Invention on the Timidine Incorporation in Human Adenocarcinoma HT29 Cells
  • Human adenocarcinoma cells are sown on plates having 24 wells (2.5×105 cells/plate) with a standard culture medium.
  • After 24 hours some plates are inoculated with the tested compounds dissolved in dimethylsulphoxide at a 200 μM concentration and others are treated with the tested compounds dissolved in dimethylsulphoxide at a 200 μM concentration in the presence of a solution of cisplatinum 25 μM. After 15 hours of incubation the plates are put into contact with a solution of 3H-timidine 1 μCi/mol (RAS. 3,000 Ci/mol).
  • The cell monolayer of each plate is first washed twice with a cold saline buffer, then treated with TCA (trichloroacetic acid) at 5% for 10 minutes and then washed three times with absolute alcohol. The cells of each well are dissolved in 500 μl of NaOH 0.1N and the incorporated radioactivity is determined by scintallation counting.
  • The obtained results are reported in Table 4 and expressed as percentage of 3H-Timidine incorporated in the cells treated with the tested compounds and in the presence of Cisplatinum, considering equal to 100 the amount of 3H-Timidine incorporated in the cells treated only with Cisplatinum.
  • TABLE 1
    Activity in the prevention of the liver injury induced by
    Concavalin A
    Dose
    Treatment (mg/kg) Liver injury %
    Treated controls — 100
    Untreated controls — 2
    Paracetamol 500 160
    Nitrooxybutyl ester of the 500 8
    Paracetamol der. with ferulic
    acid
    (Ex. 8)
    Paracetamol nitrooxybutyl ester 500 10
    (Ex. 9)
    Aspirin 300 120
    Aspirin ester with 5- 300 5
    nitrooxymethyl-2-hydroxymethyl
    pyridine
    (Ex. 3)
    Aspirin ester with 3- 300 7
    nitrooxymethyl-2-hydroxymethyl
    pyridine
    (Ex. 4)
    Sulindac 200 115
    Ester sulindac with 6- 200 23
    nitrooxymethyl-2-hydroxymethyl
    pyridine
    (Ex. 19)
    Sulindac 4-nitrooxybutyl ester 200 18
    (Ex. 18)
  • TABLE 2
    Activity in vitro on the proliferation of cancerous cells
    Concentration
    Treatment (μM) Proliferation %
    Controls — 100
    Aspirin 500 100
    Nitrooxybutyl ester of the 300 50
    aspirin der.
    with ferulic acid
    (Ex. 7)
    Aspirin ester with 3- 300 40
    nitrooxymethylphenol
    (Ex. 5)
    Aspirin ester with 4- 10 0
    nitrooxymethylphenol
    (Ex. 6)
    Aspirin ester with 6- 10 0
    nitrooxymethyl-2-hydroxymethyl
    pyridine
    (Ex. 1)
    Aspirin ester with 2- 20 50
    nitrooxymethylphenol
    (Ex. 20)
    Sulindac 50 100
    Sulindac 4-nitrooxybutyl ester 50 0
    (Ex. 18)
  • TABLE 3
    Determination in vitro of the inhibitory effect on the
    proliferation of cancerous human cells of prostate cancer and of
    bladder cancer of the compounds of the invention
    Inhibition of the proliferative activity
    (%)
    Human epithelial Human epithelial
    cells of prostate cells of bladder
    Conc. cancer cancer
    Compounds (M) PNT1 A LNCaP PC3 T24 647V 1207
    Sulindac 10−6 3 17 5 0 9 0
    Nitrooxybutyl 10−5 38 74 68 82 80 74
    ester 10−4 81 88 74 93 92 88
    (Ex. 18)
    Nitrooxybutyl 10−6 0 8 4 0 0 2
    ester of the 10−5 4 33 9 0 0 0
    Ibuprofen der. 10−4 20 60 47 22 45 43
    with
    Ferulic acid
    (Ex. 16)
    Nitrooxybutyl 10−6 0 1 8 0 0 0
    ester of the 10−5 2 26 20 0 13 0
    Flurbiprofen 10−4 13 58 53 23 41 34
    der. with
    Ferulic acid
    (Ex. 17)
    Nitrooxybutyl 10−6 0 20 1 2 0 4
    ester of the 10−5 0 47 30 0 0 24
    aspirin der. 10−4 72 81 69 55 50 82
    With Ferulic
    acid
    (Ex. 7)
  • TABLE 4
    Determination in vitro of the effect of some compounds on the
    timidine incorporation in human adenocarcinoma cells
    Conc. Without With 25 μM
    Treatment (μM) Cisplatinum Cisplatinum
    Controls — 438 100
    Carrier (DMSO) — 438 100
    Salicylic acid 200 438 100
    Comparison
    3-(nitrooxymethyl) 200 246  50
    phenyl ester
    of Salicylic acid
    (Ex. 15)
    Acetylsalicylic acid 200 438 100
    Comparison
    3-(nitrooxymethyl) 200 192  46
    phenyl ester
    of Acetylsalicylic
    acid
    (Ex. 5)

Claims (14)

1. A method for treating pre-cancer or cancer diseases on an inflammatory basis in a patient in need thereof comprising administering to the patient in need thereof an effective amount of a nitroderivative or salts thereof having the following general formula (I):

A-X1-L-(W)p-NO2  (I)
wherein:
p is an integer equal to 1 or 0;
A=R-T1-, wherein
R is the radical of a precursor drug and it has the following formula:
Figure US20090075952A1-20090319-C00043
s is 0
R1 is OCOR3 being R3a C1-C5 linear or branched radical;
R6 is H;
T1=(CO),
X1=-TB-Y-TBI- wherein
TB=X, wherein X=O, S, NR1C, wherein R1C is H or a linear or branched alkyl, having from 1 to 5 carbon atoms;
TBI=(CO)tx or (X)txx, wherein tx and txx have the value of 0 or 1; with the proviso that tx=1 when txx=0; and tx=0 when txx=1; X is as above;
Y is a bivalent linking group selected from the following:
Figure US20090075952A1-20090319-C00044
wherein:
nIX is an integer in the range 0-3;
nIIX is an integer in the range 1-3;
RTIX, RTIX′, RTIIX, RTIIX′, equal to or different from each other are H or a C1-C4 linear or branched alkyl;
Y3 is a saturated, unsaturated or aromatic heterocyclic ring, having 5 or 6 atoms, containing one or two nitrogen atoms,
an alkylene group R′ wherein R′ is a C1-C20 linear or branched, optionally substituted with one or more of the following groups: —NHCOR3, wherein R3 is as above, —NH2 or —OH;
a cycloalkylene having from 5 to 7 carbon atoms, optionally substituted with R′, R′ being as above, one or more carbon atoms of the cycloalkylene ring can optionally be replaced by heteroatoms;
Figure US20090075952A1-20090319-C00045
wherein n3 is an integer from 0 to 3 and n3′ is an integer from 1 to 3;
Figure US20090075952A1-20090319-C00046
wherein n3 and n3′ have the above meaning,
Figure US20090075952A1-20090319-C00047
wherein
R4 is hydroxy, hydrogen or R5O— alkoxy, wherein R5 is a C1-C10 linear, branched or cyclic alkyl group;
R2 is a C2-C10 linear or branched alkenylene group which can contain one or more double bonds;
Figure US20090075952A1-20090319-C00048
wherein R1f=H, CH3 and nf is an integer from 0 a 6;
L=covalent bond, CO or X, X being as defined above;
W=YTO wherein YT has the same meaning of Y as defined above and YT is equal or different from Y.
2. The method according to claim 1, wherein in formula (AI), R1 is an acetyloxy group in position 2 of the ring and the free valence of the radical R is saturated with the —COOH group and the compound is known as Acetylsalicylic acid.
3. The method according to claim 1, wherein Y3 in formula (II) of the linking group Y of X1 in formula (I) is selected from the following bivalent radicals:
Figure US20090075952A1-20090319-C00049
Figure US20090075952A1-20090319-C00050
4. The method according to claim 3, wherein Y3 is an aromatic ring having 6 atoms, containing one nitrogen atom, said aromatic ring having two free valences respectively in the positions 2 and 6, or 2 and 3 or 2 and 5 with respect to the heteroatom.
5. The method according to claim 4, wherein Y3 is Y12 (pyridyl).
6. The method according to claim 1, wherein the compounds are selected from the following:
2-(acetyloxy)benzoic acid 3-(nitrooxymethyl)phenyl ester,
2-(acetyloxy)benzoic acid 4-(nitrooxymethyl)phenyl ester,
2-(acetyloxy)benzoic acid 2-(nitrooxymethyl)phenyl ester,
2-(acetyloxy)benzoic acid 6-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride, or nitrate,
2-(acetyloxy)benzoic acid 5-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride, or nitrate,
2-(acetyloxy)benzoic acid 3-(nitrooxymethyl)-2-methylpyridinyl ester hydrochloride, or nitrate,
trans-3-[4-[2-acetyloxybenzoyloxy]-3-methoxyphenyl]-2-propenoic acid 4-(nitrooxy)butyl ester.
7. The method according to claim 1 wherein the pre-cancer diseases on an inflammatory basis are those affecting the digestive or intestinal tract.
8. Method according to claim 7 wherein the pre-cancer diseases are colitis, gastritis, duodenitis, enteritis, hepatopathies.
9. The method according to claim 1 wherein the pre-cancer diseases on an inflammatory basis are those affecting urogenital, respiratory apparatus or skin districts.
10. The method according to claim 1 wherein the cancer diseases on an inflammatory basis are those affecting urogenital, respiratory apparatus, skin or digestive systems.
11. The method according to claim 10 wherein the cancer disease is colon cancer.
12. The method according to claim 10 wherein the cancer disease is bladder cancer.
13. The method according to claim 10 wherein the cancer disease is prostate cancer.
14. The method according to claim 1 wherein compounds of the invention are used in combination with chemotherapeutic drugs or in the radiotherapeutic treatment.
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Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITMI20011240A1 (en) * 2001-06-13 2002-12-13 Nicox Sa DRUGS FOR VASCULOPATHIES
ITMI20011307A1 (en) * 2001-06-21 2002-12-21 Nicox Sa DRUGS FOR EPILEPSY
ITMI20011308A1 (en) 2001-06-21 2002-12-21 Nicox Sa DRUGS FOR CHRONIC PAIN
EP1336602A1 (en) * 2002-02-13 2003-08-20 Giovanni Scaramuzzino Nitrate prodrugs able to release nitric oxide in a controlled and selective way and their use for prevention and treatment of inflammatory, ischemic and proliferative diseases
US7220749B2 (en) 2002-06-11 2007-05-22 Nitromed, Inc. Nitrosated and/or nitrosylated cyclooxygenase-2 selective inhibitors, compositions and methods of use
ITMI20021391A1 (en) 2002-06-25 2003-12-29 Nicox Sa NITRO-DERIVATIVES OF CYCLOOXYGENASE-2 INHIBITORS
ITMI20021399A1 (en) * 2002-06-25 2003-12-29 Nicox Sa CYCLOOXYGENASE INHIBITORS 2
AU2003279622A1 (en) * 2002-06-28 2004-01-19 Nitromed, Inc. Oxime and/or hydrazone containing nitrosated and/or nitrosylated cyclooxigenase-2 selective inhibitors, compositions and methods of use
US7163958B2 (en) 2002-07-03 2007-01-16 Nitromed Inc. Nitrosated nonsteroidal antiinflammatory compounds, compositions and methods of use
WO2004010945A2 (en) 2002-07-29 2004-02-05 Nitromed, Inc. Cyclooxygenase- 2 selective inhibitors, compositions and methods of use
WO2005054175A2 (en) * 2003-11-20 2005-06-16 Nicox S.A. New process for the preparation of nitrooxyderivatives of paracetamol
TW200616604A (en) 2004-08-26 2006-06-01 Nicholas Piramal India Ltd Nitric oxide releasing prodrugs containing bio-cleavable linker
CA2577490A1 (en) 2004-08-26 2006-03-16 Nicholas Piramal India Limited Prodrugs containing alkyldisulfide linker groups
CA2679955A1 (en) * 2006-10-13 2008-05-29 Reliance Life Sciences Pvt. Ltd. Novel chemotherapeutic agents against inflammation and cancer
EP2188245B1 (en) 2007-08-17 2013-11-06 Council of Scientific & Industrial Research Nitric oxide releasing derivatives of paracetamol
CA2706028A1 (en) * 2008-01-10 2009-07-16 Francesco Impagnatiello Composition comprising a nitrooxyderivative of acetaminophen and a anticonvulsant drug for the treatment of neuropathic pain
US20110263545A1 (en) 2008-05-20 2011-10-27 Naweed Muhammad Hepatoprotectant acetaminophen mutual prodrugs
US8062653B2 (en) 2009-02-18 2011-11-22 Bezwada Biomedical, Llc Controlled release of nitric oxide and drugs from functionalized macromers and oligomers
WO2010108843A1 (en) 2009-03-27 2010-09-30 Nicox S.A. Use of nitrooxyderivative of paracetamol for the treatment of muscular dystrophies
WO2011133385A2 (en) * 2010-04-16 2011-10-27 The Research Foundation Of State University Of New York Novel ether-based compounds and their use
CN111087301B (en) * 2019-12-23 2022-05-20 河南中医药大学 Synthesis method of ibuprofen caffeate and application of ibuprofen caffeate in preparation of immunosuppressive drugs
CN111087302B (en) * 2019-12-23 2022-05-20 河南中医药大学 Synthesis method of ibuprofen ferulate and application of ibuprofen ferulate in preparation of immunosuppressive drugs

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030220228A1 (en) * 1999-12-23 2003-11-27 Bandarage Ramani R. Nitrosated and nitrosylated cyclooxygenase-2 inhibitors, compositions and methods of use

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5418261B2 (en) 1971-08-23 1979-07-06
US4721709A (en) * 1984-07-26 1988-01-26 Pyare Seth Novel pharmaceutical compositions containing hydrophobic practically water-insoluble drugs adsorbed on pharmaceutical excipients as carrier; process for their preparation and the use of said compositions
IT1256345B (en) 1992-08-20 1995-12-01 NITRIC ESTERS OF PHENYLACETIC 2- (2,6-DI-HALO-PHENYLAMIN) DERIVATIVES AND PROCEDURE FOR THEIR PREPARATION
IT1256450B (en) 1992-11-26 1995-12-05 Soldato Piero Del NITRIC ESTERS WITH PHARMACOLOGICAL ACTIVITY AND PROCEDURE FOR THEIR PREPARATION
SI0722434T1 (en) * 1993-10-06 1998-12-31 Nicox S.A. Nitric esters having anti-inflammatory and/or analgesic activity and process for their preparation
HU227280B1 (en) 1994-05-10 2011-01-28 Nicox Sa Nitro compounds and their compositions having anti-inflammatory, analgesic and antithrombotic acitivities
ES2092962B1 (en) 1995-04-19 1997-07-16 Prodes Sa NITRIC ESTERS OF ACID DERIVATIVES 2- (2,6-DIHALOFENILAMINO) FENILACETOXIACETICO AND ITS PREPARATION PROCEDURES.
DE19515970A1 (en) 1995-05-02 1996-11-07 Bayer Ag Acetylsalicylsäurenitrate
US6057139A (en) * 1995-06-29 2000-05-02 Mcneil-Ppc, Inc. Preblend of microcrystalline cellulose and lactase for making tablets
JP4286332B2 (en) 1995-07-27 2009-06-24 富士通株式会社 Method and apparatus for automatic removal of vector part contained in DNA base sequence
IT1276071B1 (en) * 1995-10-31 1997-10-24 Nicox Ltd ANTI-INFLAMMATORY ACTIVITY COMPOSITES
IT1288123B1 (en) 1996-09-04 1998-09-10 Nicox Sa USE OF NITRO-DERIVATIVES FOR URINARY INCONTINENCE
SE9704400D0 (en) 1997-11-28 1997-11-28 Astra Ab Porous inorganic particles as carriers for drug substances
SE9704401D0 (en) 1997-11-28 1997-11-28 Astra Ab Matrix pellets for greasy, oily or sticky drug substances
AU763000B2 (en) * 1998-10-30 2003-07-10 Nicox S.A. Nitrosated and nitrosylated nonsteroidal antiinflammatory compounds, compositions and methods of use
IT1307928B1 (en) * 1999-01-26 2001-11-29 Nicox Sa METHOD OF SYNTHESIS OF NITROXYMETHYLPHENYL ESTERS OF ASPIRINE DERIVATIVES.
IT1308633B1 (en) * 1999-03-02 2002-01-09 Nicox Sa NITROSSIDERIVATI.
IT1311924B1 (en) * 1999-04-13 2002-03-20 Nicox Sa PHARMACEUTICAL COMPOUNDS.
IT1311923B1 (en) 1999-04-13 2002-03-20 Nicox Sa PHARMACEUTICAL COMPOUNDS.
DE19926500C2 (en) 1999-06-10 2001-09-20 Infineon Technologies Ag Non-volatile semiconductor memory cell with a dielectric layer having a high relative dielectric constant and method for the production thereof
IT1312115B1 (en) 1999-06-24 2002-04-04 Nicox Sa AMORPHOUS COMPOUNDS AND RELATED PHARMACEUTICAL COMPOSITIONS
ITMI991517A1 (en) 1999-07-09 2001-01-09 Nicox Sa PROCEDURE FOR OBTAINING EXTERNAL NITROXYMETHYL FENYL OF SALICYLIC ACID DERIVATIVES
IT1314184B1 (en) * 1999-08-12 2002-12-06 Nicox Sa PHARMACEUTICAL COMPOSITIONS FOR THE THERAPY OF STRESS-OXIDATIVE CONDITIONS
SE0000774D0 (en) 2000-03-08 2000-03-08 Astrazeneca Ab New formulation
SE0000773D0 (en) 2000-03-08 2000-03-08 Astrazeneca Ab New formulation
US6538033B2 (en) * 2000-08-29 2003-03-25 Huntington Medical Research Institutes Nitric oxide donor compounds
SE0102993D0 (en) 2001-09-07 2001-09-07 Astrazeneca Ab New self emulsifying drug delivery system
SE0200895D0 (en) 2002-03-22 2002-03-22 Astrazeneca Ab New pharmaceutical composition
EP1558559A1 (en) 2002-09-20 2005-08-03 Nicox S.A. Manufacturing process for no-donating compounds such as no-donating diclofenac

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030220228A1 (en) * 1999-12-23 2003-11-27 Bandarage Ramani R. Nitrosated and nitrosylated cyclooxygenase-2 inhibitors, compositions and methods of use

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