US20050282164A1 - Ultraphobic sample carrier having functional hydrophilic and/or oleophilic areas - Google Patents
Ultraphobic sample carrier having functional hydrophilic and/or oleophilic areas Download PDFInfo
- Publication number
- US20050282164A1 US20050282164A1 US10/505,617 US50561705A US2005282164A1 US 20050282164 A1 US20050282164 A1 US 20050282164A1 US 50561705 A US50561705 A US 50561705A US 2005282164 A1 US2005282164 A1 US 2005282164A1
- Authority
- US
- United States
- Prior art keywords
- membrane according
- hydrophilic
- ultraphobic surface
- membrane
- areas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000012528 membrane Substances 0.000 claims description 58
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 claims description 30
- 239000000126 substance Substances 0.000 claims description 28
- 239000011159 matrix material Substances 0.000 claims description 25
- 108090000623 proteins and genes Proteins 0.000 claims description 20
- 102000004169 proteins and genes Human genes 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 239000002904 solvent Substances 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 9
- PCMORTLOPMLEFB-ONEGZZNKSA-N sinapic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC(OC)=C1O PCMORTLOPMLEFB-ONEGZZNKSA-N 0.000 claims description 8
- 239000000758 substrate Substances 0.000 claims description 7
- BRARRAHGNDUELT-UHFFFAOYSA-N 3-hydroxypicolinic acid Chemical compound OC(=O)C1=NC=CC=C1O BRARRAHGNDUELT-UHFFFAOYSA-N 0.000 claims description 6
- 239000007791 liquid phase Substances 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims description 4
- 230000002209 hydrophobic effect Effects 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 238000011160 research Methods 0.000 claims description 4
- PCMORTLOPMLEFB-UHFFFAOYSA-N sinapinic acid Natural products COC1=CC(C=CC(O)=O)=CC(OC)=C1O PCMORTLOPMLEFB-UHFFFAOYSA-N 0.000 claims description 4
- 238000012876 topography Methods 0.000 claims description 4
- XLEYFDVVXLMULC-UHFFFAOYSA-N 2',4',6'-trihydroxyacetophenone Chemical compound CC(=O)C1=C(O)C=C(O)C=C1O XLEYFDVVXLMULC-UHFFFAOYSA-N 0.000 claims description 2
- 230000010354 integration Effects 0.000 claims description 2
- WXTMDXOMEHJXQO-UHFFFAOYSA-N 2,5-dihydroxybenzoic acid Chemical compound OC(=O)C1=CC(O)=CC=C1O WXTMDXOMEHJXQO-UHFFFAOYSA-N 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 239000000523 sample Substances 0.000 description 21
- 108020004414 DNA Proteins 0.000 description 15
- 239000010410 layer Substances 0.000 description 15
- 102000053602 DNA Human genes 0.000 description 14
- 239000007788 liquid Substances 0.000 description 14
- 238000004458 analytical method Methods 0.000 description 11
- 102000004196 processed proteins & peptides Human genes 0.000 description 11
- 108090000765 processed proteins & peptides Proteins 0.000 description 11
- 102000039446 nucleic acids Human genes 0.000 description 10
- 108020004707 nucleic acids Proteins 0.000 description 10
- 150000007523 nucleic acids Chemical class 0.000 description 10
- 239000012620 biological material Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 7
- 239000000969 carrier Substances 0.000 description 7
- 229920002477 rna polymer Polymers 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 5
- 238000004949 mass spectrometry Methods 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- 108091023037 Aptamer Proteins 0.000 description 4
- 102000053642 Catalytic RNA Human genes 0.000 description 4
- 108090000994 Catalytic RNA Proteins 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000011109 contamination Methods 0.000 description 4
- 229920001184 polypeptide Polymers 0.000 description 4
- 108091092562 ribozyme Proteins 0.000 description 4
- QAIPRVGONGVQAS-DUXPYHPUSA-N trans-caffeic acid Chemical compound OC(=O)\C=C\C1=CC=C(O)C(O)=C1 QAIPRVGONGVQAS-DUXPYHPUSA-N 0.000 description 4
- -1 acetonenitrile Substances 0.000 description 3
- 239000012790 adhesive layer Substances 0.000 description 3
- 238000003491 array Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000001704 evaporation Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- ACEAELOMUCBPJP-UHFFFAOYSA-N (E)-3,4,5-trihydroxycinnamic acid Natural products OC(=O)C=CC1=CC(O)=C(O)C(O)=C1 ACEAELOMUCBPJP-UHFFFAOYSA-N 0.000 description 2
- GETTZEONDQJALK-UHFFFAOYSA-N (trifluoromethyl)benzene Chemical compound FC(F)(F)C1=CC=CC=C1 GETTZEONDQJALK-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- WNLRTRBMVRJNCN-UHFFFAOYSA-N adipic acid Chemical compound OC(=O)CCCCC(O)=O WNLRTRBMVRJNCN-UHFFFAOYSA-N 0.000 description 2
- 239000002156 adsorbate Substances 0.000 description 2
- RWZYAGGXGHYGMB-UHFFFAOYSA-N anthranilic acid Chemical compound NC1=CC=CC=C1C(O)=O RWZYAGGXGHYGMB-UHFFFAOYSA-N 0.000 description 2
- 235000004883 caffeic acid Nutrition 0.000 description 2
- 229940074360 caffeic acid Drugs 0.000 description 2
- QAIPRVGONGVQAS-UHFFFAOYSA-N cis-caffeic acid Natural products OC(=O)C=CC1=CC=C(O)C(O)=C1 QAIPRVGONGVQAS-UHFFFAOYSA-N 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 230000005661 hydrophobic surface Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000001819 mass spectrum Methods 0.000 description 2
- 239000002831 pharmacologic agent Substances 0.000 description 2
- 230000000704 physical effect Effects 0.000 description 2
- SIOXPEMLGUPBBT-UHFFFAOYSA-N picolinic acid Chemical compound OC(=O)C1=CC=CC=N1 SIOXPEMLGUPBBT-UHFFFAOYSA-N 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- XIROXSOOOAZHLL-UHFFFAOYSA-N 2',3',4'-Trihydroxyacetophenone Chemical compound CC(=O)C1=CC=C(O)C(O)=C1O XIROXSOOOAZHLL-UHFFFAOYSA-N 0.000 description 1
- XXMFJKNOJSDQBM-UHFFFAOYSA-N 2,2,2-trifluoroacetic acid;hydrate Chemical compound [OH3+].[O-]C(=O)C(F)(F)F XXMFJKNOJSDQBM-UHFFFAOYSA-N 0.000 description 1
- WRRQHEMZOCFTQP-UHFFFAOYSA-N 2,2,2-trihydroxy-1-phenylethanone Chemical compound OC(O)(O)C(=O)C1=CC=CC=C1 WRRQHEMZOCFTQP-UHFFFAOYSA-N 0.000 description 1
- AJHPGXZOIAYYDW-UHFFFAOYSA-N 3-(2-cyanophenyl)-2-[(2-methylpropan-2-yl)oxycarbonylamino]propanoic acid Chemical compound CC(C)(C)OC(=O)NC(C(O)=O)CC1=CC=CC=C1C#N AJHPGXZOIAYYDW-UHFFFAOYSA-N 0.000 description 1
- NKOPQOSBROLOFP-UHFFFAOYSA-N 6-methyl-3-sulfanylidene-2h-1,2,4-triazin-5-one Chemical compound CC1=NNC(=S)NC1=O NKOPQOSBROLOFP-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 102000002068 Glycopeptides Human genes 0.000 description 1
- 108010015899 Glycopeptides Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 108091093037 Peptide nucleic acid Proteins 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 229910006069 SO3H Inorganic materials 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- PQLVXDKIJBQVDF-UHFFFAOYSA-N acetic acid;hydrate Chemical compound O.CC(O)=O PQLVXDKIJBQVDF-UHFFFAOYSA-N 0.000 description 1
- 239000001361 adipic acid Substances 0.000 description 1
- 235000011037 adipic acid Nutrition 0.000 description 1
- 238000005571 anion exchange chromatography Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 229940077731 carbohydrate nutrients Drugs 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000005277 cation exchange chromatography Methods 0.000 description 1
- 125000003636 chemical group Chemical group 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 150000001841 cholesterols Chemical class 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 description 1
- 235000001785 ferulic acid Nutrition 0.000 description 1
- 229940114124 ferulic acid Drugs 0.000 description 1
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 description 1
- 239000010408 film Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 125000003473 lipid group Chemical group 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000001906 matrix-assisted laser desorption--ionisation mass spectrometry Methods 0.000 description 1
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- XUZLXCQFXTZASF-UHFFFAOYSA-N nitro(phenyl)methanol Chemical compound [O-][N+](=O)C(O)C1=CC=CC=C1 XUZLXCQFXTZASF-UHFFFAOYSA-N 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 125000004437 phosphorous atom Chemical group 0.000 description 1
- 229940081066 picolinic acid Drugs 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000000941 radioactive substance Substances 0.000 description 1
- 230000001846 repelling effect Effects 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00279—Features relating to reactor vessels
- B01J2219/00306—Reactor vessels in a multiple arrangement
- B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
- B01J2219/00315—Microtiter plates
- B01J2219/00317—Microwell devices, i.e. having large numbers of wells
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00378—Piezoelectric or ink jet dispensers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/00527—Sheets
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/00527—Sheets
- B01J2219/00529—DNA chips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00585—Parallel processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00596—Solid-phase processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00608—DNA chips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00614—Delimitation of the attachment areas
- B01J2219/00617—Delimitation of the attachment areas by chemical means
- B01J2219/00619—Delimitation of the attachment areas by chemical means using hydrophilic or hydrophobic regions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00623—Immobilisation or binding
- B01J2219/00626—Covalent
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00632—Introduction of reactive groups to the surface
- B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
- B01J2219/00644—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being present in discrete locations, e.g. gel pads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00677—Ex-situ synthesis followed by deposition on the substrate
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/00702—Processes involving means for analysing and characterising the products
- B01J2219/00707—Processes involving means for analysing and characterising the products separated from the reactor apparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00725—Peptides
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/0074—Biological products
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/0074—Biological products
- B01J2219/00743—Cells
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
- B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
- B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
- B01L2300/166—Suprahydrophobic; Ultraphobic; Lotus-effect
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
- C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
Definitions
- the present invention concerns a membrane with an ultraphobic surface and with at least one hydrophilic and/or oleophilic area which shows a further functionality in addition to the affinity for water and/or oil. Furthermore the present invention concerns a process for manufacturing the membrane according to the invention and its use.
- microtiterplates or sample carriers are known from the current state of technology for such tests which, for example, show a multitude of regularly-spaced wells.
- Sample carriers are known from WO 98/45406 and DE 196128 928 whose surfaces are hydrophobic and are incorporated in the hydrophilic wells.
- a sample carrier with an hydrophobic surface is known from the German patent publication DE 197 54 978.
- Hydrophilic anchor areas are incorporated in these hydrophobic surfaces.
- state of the art sample carriers have the disadvantage that the hydrophilic areas can only be made relatively expensively, especially however, that the series tests with them must be performed only relatively expensively.
- the purpose of the present invention is to make available a membrane which does not show the disadvantages of the state of the art.
- This purpose is solved according to the invention with a membrane with an ultraphobic surface and with at least one hydrophilic and/or oleophilic area which shows at least one other functionality in addition to the affinity for water and oil.
- a membrane for the purpose of the invention is any moulded padding with a surface of any shape.
- the membrane is a plate with a level surface, especially preferred is a sample carrier which preferably however shows no recesses.
- the most preferred membrane according to the invention is a film showing an ultraphobic surface.
- the surface of the membrane according to the invention is basically planar; i.e. it shows the necessary topography for an ultraphobic surface, however no microvolumes in which liquid can gather
- the membrane shows an ultraphobic surface.
- An ultraphobic surface for the purpose of the invention is distinguished in that the contact angle of a drop of water and/or oil that is on the surface is more than 150°, preferably more than 160°, and especially preferred more than 170° and the receding angle does not exceed 10°.
- Receding angle means the angle of inclination of a basically planar but structured surface from the horizontal, at which a stationary drop of water and/or oil with a volume of 10 ⁇ l is moved due to gravity for an inclination of the surface.
- Such an ultraphobic surface is described in the international patent registration WO 00/39249, which is hereby incorporated as a reference and thus counts as part of the disclosure.
- the membrane also shows hydrophilic and/or oleophilic areas.
- Hydrophilic and/or oleophilic areas for the purpose of the invention are areas on which a drop of water or oil can be deposited; i.e. a drop of water or oil, which is brought into contact with the hydrophilic and/or oleophilic area by a pipette system, remains there and detaches itself from the pipette system.
- a drop of water or oil with a volume of 10 ⁇ l on the hydrophilic and or oleophilic areas have a contact angle ⁇ 120°, preferably ⁇ 110°, especially preferred ⁇ 90° and/or the receding angle of this drop exceeds 10°.
- the hydrophilic and/or oleophilic areas show at least one other functionality in addition to the affinity for water and/or oil.
- a further functionality for the purpose of the invention is any other chemical and/or physical characteristic which the material of the hydrophilic and/or oleophilic areas shows in addition to repelling water or oil and which an be used technically.
- the areas can show at least one surface
- the hydrophilic and/or oleophilic areas are respectively completely enclosed by an ultraphobic area. Using this embodiment it is possible to anchor a drop of liquid metered on to the hydrophilic and/or oleophilic area there relatively firmly.
- hydrophilic and/or oleophilic areas are arranged on the ultraphobic surface according to a very specific pattern.
- a raster a so-called array, can be produced in which the hydrophilic and/or oleophilic areas can then be easily moved towards for series tests by, for example, a machine.
- the hydrophilic and/or oleophilic areas can show any shape and size. Preferably, however, they have an area from 1 ⁇ m 2 -10 mm 2 . A drop of liquid with a diameter of preferably 5 nm-5 mm can be deposited on such an area and preferably anchored so that it does not detach itself hanging down from the membrane according to the invention. Preferably the hydrophilic and oleophilic areas have a minimum distance of 10 ⁇ m from each other.
- the hydrophilic and/or oleophilic areas can be incorporated in any conventional way in the membrane or applied on the ultraphobic surface.
- the hydrophilic and/or oleophilic area is respectively at least one deposit on the ultraphobic surface.
- This deposit can be liquid or solid. In the case of a liquid deposit, it must preferably at least at room temperature not be or be only slightly volatile.
- the deposited substance can be produced for example using a corresponding temperature of the ultraphobic surface or using substances which are preferably detached and/or suspended on the ultraphobic surface preferably in the form of drops, and for which the solvent or liquid phase is then evaporated. In doing so the ultraphobic surface must be wettable by the solvent or the liquid phase.
- the solid deposit can also be a thin film of a solid substance.
- the deposit can also be an adsorbate whose layer thickness is only a fraction of a monolayer or layers several molecules thick.
- the deposit can for example be detached again from the ultraphobic surface with the corresponding solvents so that the ultraphobic surface can be re-used.
- the hydrophilic and/or oleophilic area shows the additional functionality of a MALDI matrix; i.e. the hydrophilic and/or oleophilic area is simultaneously a is MALDI matrix for performing the so-called MALDI mass spectrometry which is described for example in Nordhoffet al. “MALDI-MS as a new method for the analysis of nucleic acid (DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) with molecular masses up to 150,000 Dalton, Application of modern mass spectrometric methods to plant science research”, Oxford University Press (1996) pages 86-101. This publication is hereby incorporated as a reference and thus counts as part of the disclosure.
- Preferred MALDI matrices are 3-hydroxypicolinic acid, ⁇ -cyano-4-hydroxycoumarin acid, 2.5 dibydroxybenzoic acid, sinapic acid, 2 4 6 trihydroxyacetophenone nitrobenzyl alcohol, nicotinic acid, ferulic acid, caffeic acid, 2-aminobenzoic acid, picolinic acid, 3-aminobenzoic acid, 2,3,4-trihydroxyacetophenone, 6-aza-2-thiothymine, urea, succinic acid, adipic acid, malonic acid or their mixture.
- MALDI matrices are dissolved for example in acetonenitrile and preferably applied on the ultraphobic surface as drops of liquid and the solvent is then evaporated there so that the MALDI matrix is available preferably as a crystallised structure at certain points on the ultraphobic surface and thus shows the hydrophilic and/or oleophilic areas on which the samples to be analysed can be metered.
- the membrane which especially preferred is a sample carrier, shows many places each with a MALDI matrix, which are respectively completely enclosed by the ultraphobic surface.
- a sample carrier can show the same matrix or different matrices at these places.
- the ultraphobic surface non-wetted samples to be analysed are usually metered as liquid on the preferably crystalline MALDI matrices and preferably apply a little solvent at least partly to them. While the solvent is evaporating again the MALDI matrix crystallises again and the sample molecules to be analysed are embedded in the MALDI matrix or bond to the surface of the MALDI matrix. The samples so prepared can then be analysed with a corresponding mass spectrometer.
- This preferred embodiment of the present invention has the advantage that sample carriers can be made available on which the MALDI matrix or several MALDI matrices already exists at predefined positions. The user still has to only apply the samples to be analysed to the respective MALDI matrices so that the analysis is significantly simplified for him. He does not have to make and store the MALDI matrices and also does not have to keep any equipment with which the MALDI matrices can be applied to the sample carriers and dried.
- the MALDI matrix which also represents the hydrophilic and/or oleophilic area according to the invention of the membrane, has the effect of an anchor for the sample liquid which does not wet the ultraphobic surface and therefore does not come into contact with this so that the sample cannot be contaminated by substances on the ultraphobic surface.
- This is especially very important for MALDI mass spectrometry because the sample liquid is evaporated and in doing so the existing contaminations have increased solvent concentration. Therefore high-quality mass spectrums can be reproducibly determined with the membranes according to the invention.
- the ultraphobic surfaces can be cleaned after each use and used again.
- the hydrophilic and/or oleophilic areas also still show the functionality of an affinity matrix; i.e. the hydrophilic and/or oleophilic areas are simultaneously also affinity matrices.
- An affinity matrix for the purpose of the invention only bonds certain molecules of a molecule mixture. The bonding can be reversible or irreversible. The bonded molecules can be separated for example by washing the mixture and then analysed.
- the selectively bonded molecules are biomolecules and/or biological material, particularly DNA, RNA (ribonucleic acid), nucleic acid, nucleic acid analoga, spiegelmers, aptamers, ribozymes, polypeptides, peptides and/or proteins.
- the affinity matrix which simultaneously represents the hydrophilic and/or oleophilic area is preferably crystalline.
- Examples for an affinity matrix are made from the conventional solid phase chromatography chemical groups such as e.g.: anion exchange chromatography: —NH 2 , —(CH 2 ) 4 —NH 2 or (CH 2 ) 6 —NH 2 or cation exchange chromatography; —C 6 H 4 —SO 3 H or reversed phase chromatography: —(CH 2 ) 3 —CH 3 , —(CH 2 ) 7 CH 3 , —(CH 2 ) 17 —CH 3 .
- the affinity matrix is simultaneously also still a MALDI matrix.
- Such substances are for example ⁇ -cyano-4-hydroxycoumarin acid, 2,4,6 trihydroxyacetophenone, caffeic acid, sinapic acid or their mixture.
- the affinity matrices are dissolved for example in acetone, acetone/acetic acid water mixture, acetonenitrile, ethanol, isopropanol or their mixture and preferably applied on the ultraphobic surface as drops of liquid and then the solvent is evaporated there so that the affinity matrices exist preferably as a crystalline structure at certain points on the ultraphobic surface and thus represent the hydrophilic and/or oleophilic areas on which the samples to be separated and then to be analysed can be metered.
- a biomolecule for the purpose of the present invention is any molecule that any virus or organism with one or more cells is made from during its lifecycle.
- Biomolecules contain at least one oxygen, nitrogen, sulphur and/or phosphorous atom.
- the following could be named as biomolecules: aptamers, ribozymes, peptides, polypeptides, proteins, antibodies, nucleic acids, nucleic acid analogas, DNA, double-stranded DNA, RNA, double-stranded RNA/DNA, vitamins, carbohydrates, hormones, glycopeptides, glycoproteins, lipides, fatty acids and cholesterols.
- Biomaterial for the purpose of the invention contains at least one biomolecule.
- biomolecules In this connection it can also be a matter of large quantities of the same or different biomolecules. These can exist unorganised next to each other or form functional units due to change effects. Examples of these are complex proteins, genomes, cell nucleii, ribosomes, cells, united cell structures, membranes or complete organisms.
- This preferred embodiment of the present invention has the advantage that sample carriers can be made available on which an affinity matrix or several affinity matrices already exist at defined positions.
- Different affinity matrices on a sample carrier have the advantage that different molecules can be selectively bonded respectively on each affinity matrix.
- the user only has to still apply the sample liquid to be separated on each affinity matrix so that the separation of individual bondings is significantly simplified for him. He does not have to make and store the affinity matrices and also does not have to keep any equipment with which the affinity matrices are applied to the sample carrier and dried.
- the affinity matrix which also represents the hydrophilic and/or oleophilic area according to the invention of the membrane, has the effect of an anchor for the sample liquid which does not wet the ultraphobic surface and therefore does not come into contact with this so that bonded molecules on the affinity matrix cannot be contaminated by substances on the ultraphobic surface.
- the selectively bonded molecules on the affinity matrix are preferably biomolecules and/or biological materials, especially preferred are nucleic acids, nucleic acid analogas, spiegelmers, aptamers, ribozymes, polypeptides, peptides and/or proteins.
- the bonded molecules can be analysed afterwards or are available for further reactions.
- the ultraphobic surfaces can be cleaned after each use and used again.
- the hydrophilic and/or oleophilic area is at least one substrate on which at least one molecule, preferably a biomolecule and/or biological material, can be irreversibly or reversibly bonded covalently.
- substrates are polyacrylamide, polyethyleneglycol, polyvinyl alcohol, agarose, nylon, nitrocellulose and/or methylcellulose.
- the substrate shows a three-dimensional preferably porous structure whose pores are preferably 1-100 nm, especially preferred 1-20 nm and very particularly preferred 1-10 nm.
- Other examples for such substrates are inorganic bonds. Any substances can be bonded to these substrates.
- the substances are certain selected biomolecules and/or biological materials which are immobilised on the substrates and are then available for further biochemical or biological experiments or can be stored.
- biomolecule chips also called biomolecule arrays for example can be made available on which preferably several different biomolecules and/or biological materials preferably however of the same genus are immobilised.
- biomolecule chips are also an object of the present invention.
- Preferred biomolecules are DNA, RNA, peptides and/or proteins.
- DNA or protein chips can be made with these biomolecules in which many different DNA or protein molecules are bonded in the form of a defined raster.
- the immobilised molecules on these chips are then incubated with a sample solution.
- this operation is also known as hybridisation.
- proteins are immobilised, particularly protein-protein, protein-DNA, protein-RNA or the change effect of the immobilised proteins with pharmacological agents are interesting.
- both the immobilisation of the molecules as well as their further use takes place on an ultraphobic surface so that a contamination of the molecules with substances stored on the ultraphobic surface is practically impossible and background signals are basically reduced during the analysis of the immobilised molecules, preferably biomolecules.
- the ultraphobic surfaces can be cleaned after each use and used again.
- the hydrophilic and/or oleophilic area is a biomolecule or biological material which is applied preferably dissolved and/or suspended to the ultraphobic surface and for which the solvent or the liquid phase is then evaporated.
- the solvent or the liquid phase must wet the ultraphobic surface.
- biomolecules or biological materials immobilised in this way are then available for example for further biochemical or biological experiments.
- biomolecule chips also called biomolecule arrays for example can be made available on which preferably several different biomolecules preferably of the same genus are immobilised.
- the biomolecule chips are also an object of the present invention.
- Preferred biomolecules are DNA, RNA, peptides and/or proteins.
- DNA or protein chips can be made with these biomolecules in which many different DNA or protein molecules are bonded in the form of a defined raster.
- the immobilised molecules on these chips are then incubated with a sample solution.
- this operation is also known as hybridisation.
- proteins are immobilised, particularly protein-protein, protein-DNA, protein-RNA or the change effect of the immobilised proteins with pharmacological agents are interesting. It is essential to the invention that both the immobilisation of the molecules as well as their further use takes place on an ultraphobic surface so that a contamination of the molecules with substances stored on the ultraphobic surface is practically impossible and background signals are basically reduced during the analysis.
- the biomolecules adhere directly to the ultraphobic surface without another substance so that the manufacture of these membranes is especially simple and economic.
- the ultraphobic surfaces can be cleaned after each use and used again.
- the membranes according to the invention are suitable for the analysis of any kind of liquid such as those known for example from chemical research or biotechnology.
- the membrane according to the invention is particularly suitable for expression, mass spectrometric and/or optical analysis of biomolecules and/or biological materials especially nucleic acids, nucleic-acid analogas, spiegelmers, aptamers, ribozymes, polypeptides, proteins and/or peptides.
- hydrophilic and/or oleophilic area can also be a peptide nucleic acid which is hybridised with single-stranded DNA.
- Another object of the present invention is a process for manufacturing the membrane according to the invention in which a functional substance on the respective ultraphobic surface which is dissolved and/or suspended in a solvent which wets the ultraphobic surface is preferably metered in drops and the liquid phase or the solvent is evaporated afterwards.
- the process according to the invention is simple and economic to execute.
- the functional substance which simultaneously represents the hydrophilic and/or oleophilic areas, can be detached again after each use and the ultraphobic surface used again.
- the ultraphobic surface Preferably several different substances are metered onto the ultraphobic surface so that so-called arrays can be produced.
- the functional substances are MALDI matrices, affinity matrices, biomolecules especially DNAs, proteins and/or bond molecules. DNA or protein chips can thus be manufactured with the process according to the invention.
- the ultraphobic surface is designed as disposable.
- This membrane has the advantage that the first layer with the ultraphobic surface can be detached from the carrier after one or after several uses and can be replaced with a new first layer so that it is impossible that this first layer is contaminated by previous experiments.
- the first layer with the ultraphobic surface is particularly economic to make as disposable. As a result of the defined flatness according to the invention, it is guaranteed that the membrane can be used in all current mass spectrometers and/or optical analysis equipment.
- the first layer is glued on to the carrier.
- the preferred membrane has diverse uses, however it is especially suitable for mass spectroscopic and/or optical analyses.
- a roller-polished AlMg 3 sheet with a surface of 26 mm ⁇ 76 mm and a thickness of 0.15 mm was degreased at room temperature with chloroform (p.a.) and afterwards 20 s in aqueous NaOH (5 g/l) at 50° C. Afterwards it was pickled in H 2 PO 4 (100 g/l) for 20 s, rinsed in distilled water for 30 s and electrochemically pickled for 90 s in a mixture of HCl/H 3 BO 3 (4 g/l each) at 35° C. and 120 mA/cm 2 at 35 V alternating current.
- the sheet treated like this was coated with an approx. 40 nm thick layer of gold using cathodic evaporation in the high vacuum. Finally the sample was coated with a monolayer by immersion for 24 hours in a solution of the thiol CF 3 —(CF 2 ) 7 —(CH 2 ) 2 —SH in benzotrifluoride (p.a. 1 g/l) at room temperature in a closed container, rinsed afterwards with benzotrifluoride (p.a.) and dried.
- the surface shows a static contact angle of 178° for water. On inclining the surface by ⁇ 2° a drop of water with a volume of 10 ⁇ l rolls away.
- a sample carrier with a surface according to Example 1 is used for this example.
- Various aliquots of MALDI matrices e.g. 3-hydroxypicolinic acid, sinapic acid and ⁇ -cyano-4-hydroxycoumarin acid dissolved in acetone, acetone nitrile or a mixture of water and one of the mentioned organic solvents, where the solvent content should be at least 50% by volume, were dispensed on the uncleaned ultraphobic surface with a piezo dispensing station. After the rapid evaporation of the solvent, all the tested matrices precipitated on the ultraphobic surface as hydrophilic areas in the form of small crystals and adhered so firmly to it that they could not be detached with either a wipe or with compressed air.
- the places populated with matrices had respectively a diameter from 200-1000 ⁇ m.
- the matrices are hydrophilic for the purpose of the invention; i.e. they show two functionalities.
- Various samples, respectively 0.5-2.0 ⁇ l which showed biomolecules were metered onto the places populated with matrices.
- the samples contained for example peptides or proteins dissolved in 0.1% TFA water or oligonucleotides dissolved in water where the content in the biomolecule amounted to 0.1-1 pmol per ⁇ l respectively.
- the samples were applied to the matrices with a hand pipette and evaporated at room temperature and analysed afterwards in a MALDI TOF mass spectrometer MTP Autoflex from the company Bruker Daltronic GmbH, 28359 Bremen in linear or reflector operation. In all cases, high-quality mass spectrums were recorded although the ultraphobic surface was not cleaned before each application. This is particularly important for analyses of nucleic acids which are falsified by the least amount of contamination for example by Na or K salts on the sample carriers.
- FIG. 1 shows the membrane 101 which consists of a first layer 201 with an ultraphobic surface 301 and a carrier 401 .
- the first layer 201 is fixed on the carrier using an adhesive layer 501 .
- the expert realises that the adhesive layer 501 does not absolutely have to be present.
- the adhesive layer 501 consists of an electrically conductive material so that there is an electrical contact between the first layer 201 and the carrier.
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- General Physics & Mathematics (AREA)
- Materials Engineering (AREA)
- Crystallography & Structural Chemistry (AREA)
- Composite Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a planar structure having an ultraphobic surface and at least one hydrophilic and/or oleophilic area that, in addition to being hydrophilic and/or oleophilic, has at least one additional functionality. The invention also relates to a method for producing the inventive planar structure and to the use thereof.
Description
- The present invention concerns a membrane with an ultraphobic surface and with at least one hydrophilic and/or oleophilic area which shows a further functionality in addition to the affinity for water and/or oil. Furthermore the present invention concerns a process for manufacturing the membrane according to the invention and its use.
- In the area of medicinal chemistry and also in biological research and production these days, cumulative series tests must be carried out. In doing so, a large number of small, liquid samples, for example, are charged with different active ingredients in order to test their reaction to the respective active ingredient.
- So-called microtiterplates or sample carriers are known from the current state of technology for such tests which, for example, show a multitude of regularly-spaced wells. Sample carriers are known from WO 98/45406 and DE 196128 928 whose surfaces are hydrophobic and are incorporated in the hydrophilic wells. A sample carrier with an hydrophobic surface is known from the German patent publication DE 197 54 978. Hydrophilic anchor areas are incorporated in these hydrophobic surfaces. However, state of the art sample carriers have the disadvantage that the hydrophilic areas can only be made relatively expensively, especially however, that the series tests with them must be performed only relatively expensively.
- Therefore the purpose of the present invention is to make available a membrane which does not show the disadvantages of the state of the art.
- This purpose is solved according to the invention with a membrane with an ultraphobic surface and with at least one hydrophilic and/or oleophilic area which shows at least one other functionality in addition to the affinity for water and oil.
- It was extremely astonishing for and unexpected by the expert that the membrane according to the invention succeeds in significantly simplifying series tests and performing them with low particularly mechanical complexity.
- A membrane for the purpose of the invention is any moulded padding with a surface of any shape. Preferably, however, the membrane is a plate with a level surface, especially preferred is a sample carrier which preferably however shows no recesses. The most preferred membrane according to the invention is a film showing an ultraphobic surface. Preferably the surface of the membrane according to the invention is basically planar; i.e. it shows the necessary topography for an ultraphobic surface, however no microvolumes in which liquid can gather
- According to the invention, the membrane shows an ultraphobic surface. An ultraphobic surface for the purpose of the invention is distinguished in that the contact angle of a drop of water and/or oil that is on the surface is more than 150°, preferably more than 160°, and especially preferred more than 170° and the receding angle does not exceed 10°. Receding angle means the angle of inclination of a basically planar but structured surface from the horizontal, at which a stationary drop of water and/or oil with a volume of 10 μl is moved due to gravity for an inclination of the surface. Such ultraphobic surfaces are revealed, for example, in WO 98/23549, WO 96/04123, WO 96/21523, WO 99/10323, WO 00/39368, WO 00/39239, WO 00/39051, WO 00/38845 and WO 96/34697 which are hereby incoporated as references and thus count as part of the disclosure.
- In a preferred embodiment, the ultraphobic surface shows a surface topography for which the local frequency of the individual Fourier components and whose amplitude a (f) expressed by the integral S (log(f))=a(f)*f calculated between the integration limits log(f1/μm−1)=−3 and log(f2/μm −1)=3 is at least 0.3 and which consists of an hydrophobic or particularly oleophobic material or are coated with a durable hydrophobic and/or particularly durable oleophobic material. Such an ultraphobic surface is described in the international patent registration WO 00/39249, which is hereby incorporated as a reference and thus counts as part of the disclosure.
- According to the invention, the membrane also shows hydrophilic and/or oleophilic areas. Hydrophilic and/or oleophilic areas for the purpose of the invention are areas on which a drop of water or oil can be deposited; i.e. a drop of water or oil, which is brought into contact with the hydrophilic and/or oleophilic area by a pipette system, remains there and detaches itself from the pipette system. Preferably, a drop of water or oil with a volume of 10 μl on the hydrophilic and or oleophilic areas have a contact angle <120°, preferably <110°, especially preferred <90° and/or the receding angle of this drop exceeds 10°. Furthermore, according to the invention, the hydrophilic and/or oleophilic areas show at least one other functionality in addition to the affinity for water and/or oil.
- A further functionality for the purpose of the invention is any other chemical and/or physical characteristic which the material of the hydrophilic and/or oleophilic areas shows in addition to repelling water or oil and which an be used technically. For example, but not limited to these only, the properties are mentioned here: The areas can show at least one surface,
-
- which makes a bond with other molecules;
- which catalyses chemical reactions;
- which releases at least one substance, which makes a bond with other molecules;
- which has the effect of a reagent for samples to be tested;
- which releases at least one substance, which has the effect of a reagent for samples to be tested;
- which shows a different optical property (absorption, reflection, transmittance, remittance, luminescence, diffusion) than the environment;
- which emits light when subjected to heat;
- which shows a different heat transfer ability than the environment;
- which shows a different acoustic property (e.g. sound absorption, acoustic velocity) than the environment;
- which shows a different surface friction than the environment;
- which shows a different absorption behaviour (e.g. absorption speed, equilibrium) than the environment;
- which shows a different electrical property (e.g. conductability, capacitivity) than the environment;
- which shows a different magnetic property (e.g. susceptibility) than the environment;
- which shows a different surface diffusion behaviour than the environment;
- which contains a radioactive substance;
- which shows a different α, β or γ emission than the environment;
- which shows a different specific surface than the environment;
- which shows a different surface topography than the environment;
- which shows a different porosity than the environment;
- which shows a different surface coverage of an adsorbate than the environment;
- which shows a different molecular weight of a polymer than the surrounding polymer surface;
- which shows a different electrochemical potential than the environment;
- which shows a different surface loading thickness than the environment;
- which shows a different electrokinetic potential (zeta potential) than the environment;
- which using one of the chemical or physical properties mentioned above releases at least one substance having the effect of a reagent for the samples to be tested;
- which using one of the chemical or physical properties mentioned above releases at least one substance which bonds with other molecules and/or splits molecules e.g. biomolecules specifically or not specifically.
- Preferably the hydrophilic and/or oleophilic areas are respectively completely enclosed by an ultraphobic area. Using this embodiment it is possible to anchor a drop of liquid metered on to the hydrophilic and/or oleophilic area there relatively firmly.
- Also preferred is that the hydrophilic and/or oleophilic areas are arranged on the ultraphobic surface according to a very specific pattern. Thus, for example, a raster, a so-called array, can be produced in which the hydrophilic and/or oleophilic areas can then be easily moved towards for series tests by, for example, a machine.
- The hydrophilic and/or oleophilic areas can show any shape and size. Preferably, however, they have an area from 1 μm2-10 mm2. A drop of liquid with a diameter of preferably 5 nm-5 mm can be deposited on such an area and preferably anchored so that it does not detach itself hanging down from the membrane according to the invention. Preferably the hydrophilic and oleophilic areas have a minimum distance of 10 μm from each other.
- The hydrophilic and/or oleophilic areas can be incorporated in any conventional way in the membrane or applied on the ultraphobic surface. Preferably, however, the hydrophilic and/or oleophilic area is respectively at least one deposit on the ultraphobic surface. This deposit can be liquid or solid. In the case of a liquid deposit, it must preferably at least at room temperature not be or be only slightly volatile. The deposited substance can be produced for example using a corresponding temperature of the ultraphobic surface or using substances which are preferably detached and/or suspended on the ultraphobic surface preferably in the form of drops, and for which the solvent or liquid phase is then evaporated. In doing so the ultraphobic surface must be wettable by the solvent or the liquid phase. Details for this can be found from the parallel registrations with the internal file numbers Sy 0028 and Sy 0029 lodged with the German patent and trademark authority which are hereby incorporated as references and thus count as part of the disclosure. The solid deposit can also be a thin film of a solid substance. The deposit can also be an adsorbate whose layer thickness is only a fraction of a monolayer or layers several molecules thick. The deposit can for example be detached again from the ultraphobic surface with the corresponding solvents so that the ultraphobic surface can be re-used.
- Preferably the hydrophilic and/or oleophilic area shows the additional functionality of a MALDI matrix; i.e. the hydrophilic and/or oleophilic area is simultaneously a is MALDI matrix for performing the so-called MALDI mass spectrometry which is described for example in Nordhoffet al. “MALDI-MS as a new method for the analysis of nucleic acid (DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) with molecular masses up to 150,000 Dalton, Application of modern mass spectrometric methods to plant science research”, Oxford University Press (1996) pages 86-101. This publication is hereby incorporated as a reference and thus counts as part of the disclosure. Preferred MALDI matrices are 3-hydroxypicolinic acid, α-cyano-4-hydroxycoumarin acid, 2.5 dibydroxybenzoic acid, sinapic acid, 2 4 6 trihydroxyacetophenone nitrobenzyl alcohol, nicotinic acid, ferulic acid, caffeic acid, 2-aminobenzoic acid, picolinic acid, 3-aminobenzoic acid, 2,3,4-trihydroxyacetophenone, 6-aza-2-thiothymine, urea, succinic acid, adipic acid, malonic acid or their mixture. These MALDI matrices are dissolved for example in acetonenitrile and preferably applied on the ultraphobic surface as drops of liquid and the solvent is then evaporated there so that the MALDI matrix is available preferably as a crystallised structure at certain points on the ultraphobic surface and thus shows the hydrophilic and/or oleophilic areas on which the samples to be analysed can be metered.
- Preferably the membrane, which especially preferred is a sample carrier, shows many places each with a MALDI matrix, which are respectively completely enclosed by the ultraphobic surface. A sample carrier can show the same matrix or different matrices at these places.
- The ultraphobic surface non-wetted samples to be analysed are usually metered as liquid on the preferably crystalline MALDI matrices and preferably apply a little solvent at least partly to them. While the solvent is evaporating again the MALDI matrix crystallises again and the sample molecules to be analysed are embedded in the MALDI matrix or bond to the surface of the MALDI matrix. The samples so prepared can then be analysed with a corresponding mass spectrometer.
- This preferred embodiment of the present invention has the advantage that sample carriers can be made available on which the MALDI matrix or several MALDI matrices already exists at predefined positions. The user still has to only apply the samples to be analysed to the respective MALDI matrices so that the analysis is significantly simplified for him. He does not have to make and store the MALDI matrices and also does not have to keep any equipment with which the MALDI matrices can be applied to the sample carriers and dried. The MALDI matrix, which also represents the hydrophilic and/or oleophilic area according to the invention of the membrane, has the effect of an anchor for the sample liquid which does not wet the ultraphobic surface and therefore does not come into contact with this so that the sample cannot be contaminated by substances on the ultraphobic surface. This is especially very important for MALDI mass spectrometry because the sample liquid is evaporated and in doing so the existing contaminations have increased solvent concentration. Therefore high-quality mass spectrums can be reproducibly determined with the membranes according to the invention. The ultraphobic surfaces can be cleaned after each use and used again.
- In another preferred embodiment of the present invention, the hydrophilic and/or oleophilic areas also still show the functionality of an affinity matrix; i.e. the hydrophilic and/or oleophilic areas are simultaneously also affinity matrices. An affinity matrix for the purpose of the invention only bonds certain molecules of a molecule mixture. The bonding can be reversible or irreversible. The bonded molecules can be separated for example by washing the mixture and then analysed. Preferably the selectively bonded molecules are biomolecules and/or biological material, particularly DNA, RNA (ribonucleic acid), nucleic acid, nucleic acid analoga, spiegelmers, aptamers, ribozymes, polypeptides, peptides and/or proteins. These biomolecules can be analysed for example by allowance of at least one MALDI matrix and MALDI mass spectrometry afterwards. The affinity matrix which simultaneously represents the hydrophilic and/or oleophilic area is preferably crystalline. Examples for an affinity matrix are made from the conventional solid phase chromatography chemical groups such as e.g.: anion exchange chromatography: —NH2, —(CH2)4—NH2 or (CH2)6—NH2 or cation exchange chromatography; —C6H4—SO3H or reversed phase chromatography: —(CH2)3—CH3, —(CH2)7CH3, —(CH2)17—CH3. Especially preferred however is that the affinity matrix is simultaneously also still a MALDI matrix. Such substances are for example α-cyano-4-hydroxycoumarin acid, 2,4,6 trihydroxyacetophenone, caffeic acid, sinapic acid or their mixture. The affinity matrices are dissolved for example in acetone, acetone/acetic acid water mixture, acetonenitrile, ethanol, isopropanol or their mixture and preferably applied on the ultraphobic surface as drops of liquid and then the solvent is evaporated there so that the affinity matrices exist preferably as a crystalline structure at certain points on the ultraphobic surface and thus represent the hydrophilic and/or oleophilic areas on which the samples to be separated and then to be analysed can be metered.
- A biomolecule for the purpose of the present invention is any molecule that any virus or organism with one or more cells is made from during its lifecycle. Biomolecules contain at least one oxygen, nitrogen, sulphur and/or phosphorous atom. For example, the following could be named as biomolecules: spiegelmers, aptamers, ribozymes, peptides, polypeptides, proteins, antibodies, nucleic acids, nucleic acid analogas, DNA, double-stranded DNA, RNA, double-stranded RNA/DNA, vitamins, carbohydrates, hormones, glycopeptides, glycoproteins, lipides, fatty acids and cholesterols.
- Biological material for the purpose of the invention contains at least one biomolecule. In this connection it can also be a matter of large quantities of the same or different biomolecules. These can exist unorganised next to each other or form functional units due to change effects. Examples of these are complex proteins, genomes, cell nucleii, ribosomes, cells, united cell structures, membranes or complete organisms.
- This preferred embodiment of the present invention has the advantage that sample carriers can be made available on which an affinity matrix or several affinity matrices already exist at defined positions. Different affinity matrices on a sample carrier have the advantage that different molecules can be selectively bonded respectively on each affinity matrix. The user only has to still apply the sample liquid to be separated on each affinity matrix so that the separation of individual bondings is significantly simplified for him. He does not have to make and store the affinity matrices and also does not have to keep any equipment with which the affinity matrices are applied to the sample carrier and dried. The affinity matrix, which also represents the hydrophilic and/or oleophilic area according to the invention of the membrane, has the effect of an anchor for the sample liquid which does not wet the ultraphobic surface and therefore does not come into contact with this so that bonded molecules on the affinity matrix cannot be contaminated by substances on the ultraphobic surface. The selectively bonded molecules on the affinity matrix are preferably biomolecules and/or biological materials, especially preferred are nucleic acids, nucleic acid analogas, spiegelmers, aptamers, ribozymes, polypeptides, peptides and/or proteins. The bonded molecules can be analysed afterwards or are available for further reactions. The ultraphobic surfaces can be cleaned after each use and used again.
- In another preferred embodiment of the present invention, the hydrophilic and/or oleophilic area is at least one substrate on which at least one molecule, preferably a biomolecule and/or biological material, can be irreversibly or reversibly bonded covalently. Examples of such substrates are polyacrylamide, polyethyleneglycol, polyvinyl alcohol, agarose, nylon, nitrocellulose and/or methylcellulose. Preferably the substrate shows a three-dimensional preferably porous structure whose pores are preferably 1-100 nm, especially preferred 1-20 nm and very particularly preferred 1-10 nm. Other examples for such substrates are inorganic bonds. Any substances can be bonded to these substrates. Preferably, however, the substances are certain selected biomolecules and/or biological materials which are immobilised on the substrates and are then available for further biochemical or biological experiments or can be stored. In this way biomolecule chips also called biomolecule arrays for example can be made available on which preferably several different biomolecules and/or biological materials preferably however of the same genus are immobilised.
- The biomolecule chips are also an object of the present invention. Preferred biomolecules are DNA, RNA, peptides and/or proteins. For example, so-called DNA or protein chips can be made with these biomolecules in which many different DNA or protein molecules are bonded in the form of a defined raster. The immobilised molecules on these chips are then incubated with a sample solution. In the case of DNA-DNA, DNA-RNA or RNA-RNA interactions this operation is also known as hybridisation. If proteins are immobilised, particularly protein-protein, protein-DNA, protein-RNA or the change effect of the immobilised proteins with pharmacological agents are interesting. It is essential to the invention that both the immobilisation of the molecules as well as their further use takes place on an ultraphobic surface so that a contamination of the molecules with substances stored on the ultraphobic surface is practically impossible and background signals are basically reduced during the analysis of the immobilised molecules, preferably biomolecules. The ultraphobic surfaces can be cleaned after each use and used again.
- In another preferred embodiment of the present invention, the hydrophilic and/or oleophilic area is a biomolecule or biological material which is applied preferably dissolved and/or suspended to the ultraphobic surface and for which the solvent or the liquid phase is then evaporated. The solvent or the liquid phase must wet the ultraphobic surface. The biomolecules or biological materials immobilised in this way are then available for example for further biochemical or biological experiments. In this way, biomolecule chips also called biomolecule arrays for example can be made available on which preferably several different biomolecules preferably of the same genus are immobilised. The biomolecule chips are also an object of the present invention. Preferred biomolecules are DNA, RNA, peptides and/or proteins. For example, so-called DNA or protein chips can be made with these biomolecules in which many different DNA or protein molecules are bonded in the form of a defined raster. The immobilised molecules on these chips are then incubated with a sample solution. In the case of DNA-DNA, DNA-RNA or RNA-RNA interactions this operation is also known as hybridisation. If proteins are immobilised, particularly protein-protein, protein-DNA, protein-RNA or the change effect of the immobilised proteins with pharmacological agents are interesting. It is essential to the invention that both the immobilisation of the molecules as well as their further use takes place on an ultraphobic surface so that a contamination of the molecules with substances stored on the ultraphobic surface is practically impossible and background signals are basically reduced during the analysis. In this embodiment of the membrane according to the invention it is particularly advantageous that the biomolecules adhere directly to the ultraphobic surface without another substance so that the manufacture of these membranes is especially simple and economic. The ultraphobic surfaces can be cleaned after each use and used again.
- The membranes according to the invention are suitable for the analysis of any kind of liquid such as those known for example from chemical research or biotechnology. The membrane according to the invention is particularly suitable for expression, mass spectrometric and/or optical analysis of biomolecules and/or biological materials especially nucleic acids, nucleic-acid analogas, spiegelmers, aptamers, ribozymes, polypeptides, proteins and/or peptides. These applications are also objects of the present invention.
- An optical analysis for the purpose of the present inventions is described in the German parallel registration with the file number DE 102 07 614 lodged with the German patent and trademark authority. Details of mass spectrometric analysis, particularly the MALDI process, can be found in the parallel registration with the file number DE 10207615 also lodged with the German patent and trademark authority. Both registrations are hereby incorporated as references and thus count as part of the disclosure.
- Furthermore, the hydrophilic and/or oleophilic area can also be a peptide nucleic acid which is hybridised with single-stranded DNA.
- Another object of the present invention is a process for manufacturing the membrane according to the invention in which a functional substance on the respective ultraphobic surface which is dissolved and/or suspended in a solvent which wets the ultraphobic surface is preferably metered in drops and the liquid phase or the solvent is evaporated afterwards.
- The process according to the invention is simple and economic to execute. The functional substance, which simultaneously represents the hydrophilic and/or oleophilic areas, can be detached again after each use and the ultraphobic surface used again.
- Preferably several different substances are metered onto the ultraphobic surface so that so-called arrays can be produced. Preferably the functional substances are MALDI matrices, affinity matrices, biomolecules especially DNAs, proteins and/or bond molecules. DNA or protein chips can thus be manufactured with the process according to the invention.
- Reference should be made to the above with respect to the application concerning MALDI matrices, affinity matrices, biomolecules and bond molecules.
- In a preferred embodiment of both membranes according to the invention, the ultraphobic surface is designed as disposable.
- A membrane with several layers with a first layer with an ultraphobic surface and a carrier, where the first layer is applied reversibly to the carrier and the maximum local flatness deviation of the membrane is 100 μm preferably <20 μm, is particularly suitable for this embodiment.
- This membrane has the advantage that the first layer with the ultraphobic surface can be detached from the carrier after one or after several uses and can be replaced with a new first layer so that it is impossible that this first layer is contaminated by previous experiments. The first layer with the ultraphobic surface is particularly economic to make as disposable. As a result of the defined flatness according to the invention, it is guaranteed that the membrane can be used in all current mass spectrometers and/or optical analysis equipment.
- In a preferred embodiment of the membrane the first layer is glued on to the carrier.
- Furthermore, it is preferred there is an electrical contact between the first layer and the carrier. This embodiment is particularly advantageous for mass spectroscopic analyses.
- The preferred membrane has diverse uses, however it is especially suitable for mass spectroscopic and/or optical analyses.
- The invention is explained in the following by means of the Examples 1-2 and
FIG. 1 . These explanations are solely for example and do not restrict the general notions of the invention. - Manufacture of the Ultraphobic Surface:
- A roller-polished AlMg3 sheet with a surface of 26 mm×76 mm and a thickness of 0.15 mm was degreased at room temperature with chloroform (p.a.) and afterwards 20 s in aqueous NaOH (5 g/l) at 50° C. Afterwards it was pickled in H2PO4 (100 g/l) for 20 s, rinsed in distilled water for 30 s and electrochemically pickled for 90 s in a mixture of HCl/H3BO3 (4 g/l each) at 35° C. and 120 mA/cm2 at 35 V alternating current.
- After 30 s rinsing in distilled water and 30 s alkaline rinsing in aqueous NaOH (5 g/l) it was rinsed again in distilled water for 30 s and anodically oxidised afterwards for 90 s in H2SO4 (200 g/l) at 25° C. with 30 mA/cm2 at 50 V direct current.
- Afterwards it was rinsed for 30 s in distilled water, then 60 s at 40° C. in NaHCO3 (20 g/l), then again 30 s in distilled water and dried for 1 hour at 80° C. in the drying cabinet.
- The sheet treated like this was coated with an approx. 40 nm thick layer of gold using cathodic evaporation in the high vacuum. Finally the sample was coated with a monolayer by immersion for 24 hours in a solution of the thiol CF3—(CF2)7—(CH2)2—SH in benzotrifluoride (p.a. 1 g/l) at room temperature in a closed container, rinsed afterwards with benzotrifluoride (p.a.) and dried.
- The surface shows a static contact angle of 178° for water. On inclining the surface by <2° a drop of water with a volume of 10 μl rolls away.
- A sample carrier with a surface according to Example 1 is used for this example. Various aliquots of MALDI matrices e.g. 3-hydroxypicolinic acid, sinapic acid and α-cyano-4-hydroxycoumarin acid dissolved in acetone, acetone nitrile or a mixture of water and one of the mentioned organic solvents, where the solvent content should be at least 50% by volume, were dispensed on the uncleaned ultraphobic surface with a piezo dispensing station. After the rapid evaporation of the solvent, all the tested matrices precipitated on the ultraphobic surface as hydrophilic areas in the form of small crystals and adhered so firmly to it that they could not be detached with either a wipe or with compressed air. The places populated with matrices had respectively a diameter from 200-1000 μm. The matrices are hydrophilic for the purpose of the invention; i.e. they show two functionalities. Various samples, respectively 0.5-2.0 μl which showed biomolecules were metered onto the places populated with matrices. The samples contained for example peptides or proteins dissolved in 0.1% TFA water or oligonucleotides dissolved in water where the content in the biomolecule amounted to 0.1-1 pmol per μl respectively. The samples were applied to the matrices with a hand pipette and evaporated at room temperature and analysed afterwards in a MALDI TOF mass spectrometer MTP Autoflex from the company Bruker Daltronic GmbH, 28359 Bremen in linear or reflector operation. In all cases, high-quality mass spectrums were recorded although the ultraphobic surface was not cleaned before each application. This is particularly important for analyses of nucleic acids which are falsified by the least amount of contamination for example by Na or K salts on the sample carriers.
-
FIG. 1 shows themembrane 101 which consists of a first layer 201 with anultraphobic surface 301 and acarrier 401. The first layer 201 is fixed on the carrier using anadhesive layer 501. The expert realises that theadhesive layer 501 does not absolutely have to be present. Theadhesive layer 501 consists of an electrically conductive material so that there is an electrical contact between the first layer 201 and the carrier.
Claims (29)
1. Membrane with an ultraphobic surface and with at least one hydrophilic and/or oleophilic area, characterised in that the hydrophilic and/or oleophilic area shows at least one further functionality in addition to the affinity for water and/or oil.
2. Membrane according to claim 1 with a multitude of hydrophilic and/or oleophilic areas.
3. Membrane according to claim 1 , characterised in that the hydrophilic and/or oleophilic areas are each completely enclosed by the ultraphobic surface.
4. Membrane according to claim 1 , characterised in that the hydrophilic and/or oleophilic areas are distributed at least partly according to a defined pattern on the ultraphobic surface.
5. Membrane according to claim 1 , characterised in that the ultraphobic surface shows a surface topography for which the local frequency f of the individual Fourier components and their amplitudes a(f) expressed by the integral S(log (f))=a(f)*f, calculated between the integration limits log(f1 μm−1)=3 and log (f2 μm−1)=3, is at least 0.3 and which consists of a hydrophobic and/or particularly durable oleophobic material.
6. Membrane according to claim 1 , characterised in that the hydrophilic and/or oleophilic areas each show an area of 1 μm2-10 μm2.
7. Membrane according to claim 1 , characterised in that the hydrophilic areas are each at least one deposit, preferably a solidified substance, on the ultraphobic surface.
8. Membrane according to claim 1 , characterised in that the hydrophilic areas are each a MALDI matrix.
9. Membrane according to claim 1 , characterised in that the hydrophilic areas are each at least one affinity matrix.
10. Membrane according to claim 1 , characterised in that the hydrophilic areas are each at least one substrate on which at least one molecule, especially a biomolecule, can be bonded.
11. Membrane according to claim 1 , characterised in that the hydrophilic areas are each at least one biomolecule, preferably a DNA and/or a protein molecule.
12. Membrane according to claim 1 , characterised in that the hydrophilic and/or oleophilic areas can be produced reversibly.
13. Membrane, preferably sample carrier, with several biomolecules which are each immobilised on an ultraphobic surface.
14. Membrane according to claim 13 , characterised in that the biomolecules are arranged in the form of a specified raster.
15. Membrane according to claim 13 , characterised in that the biomolecules are DNA and/or protein molecules.
16. Membrane, preferably sample carrier, with several MALDI matrices which are each immobilised on an ultraphobic surface.
17. Membrane according to claim 16 , characterised in that the MALDI matrices are arranged in the form of a specified raster.
18. Membrane according to claim 16 , characterised in that the MALDI matrices are 3-hydroxypicolinic acid, α-cyano-4-hydroxycoumarin acid, 2.5 dihydroxybenzoic acid, sinapic acid, 2,4,6 trihydroxyacetophenone or their mixture.
19. Membrane, preferably sample carrier, with several affinity matrices which are each immobilised on an ultraphobic surface.
20. Membrane according to claim 19 , characterised in that the affinity matrices are arranged in the form of a specified raster.
21. Membrane according to claim 1 with a first layer with an ultraphobic surface and with a carrier, characterised in that the first layer is applied reversibly on a carrier and the maximum local flatness deviation is <100 μm.
22. Membrane according to claim 21 , characterised in that the first layer is glued to the carrier.
23. Membrane according to claim 21 , characterised in that there is an electrical contact between the first layer and the carrier.
24. Membrane according to claim 21 , characterised in that the ultraphobic surface (3) shows at least one hydrophilic area.
25. Membrane according to claim 21 , characterised in that the layer is disposable.
26. Process for maufacturing a membrane according to claim 1 , characterised in that a functional substance, which is dissolved and/or suspended in a solvent that wets the ultraphobic surface is metered, preferably in drops, on to each ultraphobic surface and the liquid phase or the solvent is evaporated afterwards.
27. Process according to claim 21 , characterised in that several different substances are metered on the ultraphobic surface.
28. Process according to claim 21 , characterised in that the substances are MALDI matrices, affinity matrices, biomolecules, reagents and/or bond molecules.
29. Application of the membrane according to claim 1 in chemical research and in biotechnology.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10207616.2 | 2002-02-22 | ||
DE10207616A DE10207616A1 (en) | 2002-02-22 | 2002-02-22 | Planar structure comprises ultraphobic surface and hydrophilic or oleophilic regions having an additional functionality, useful as a carrier for e.g. binding agents |
DE10255276.2 | 2002-11-26 | ||
DE2002155276 DE10255276A1 (en) | 2002-11-26 | 2002-11-26 | Precise, contamination-free dosing of liquids onto surface with hydrophilic or oleophilic areas surrounded by ultraphobic areas comprises applying drop of liquid to hydrophilic or oleophilic areas |
PCT/EP2003/001860 WO2003070364A1 (en) | 2002-02-22 | 2003-02-24 | Ultraphobic sample carrier having functional hydrophilic and/or oleophilic areas |
Publications (1)
Publication Number | Publication Date |
---|---|
US20050282164A1 true US20050282164A1 (en) | 2005-12-22 |
Family
ID=27758408
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/505,617 Abandoned US20050282164A1 (en) | 2002-02-22 | 2003-02-24 | Ultraphobic sample carrier having functional hydrophilic and/or oleophilic areas |
Country Status (4)
Country | Link |
---|---|
US (1) | US20050282164A1 (en) |
EP (1) | EP1478456A1 (en) |
AU (1) | AU2003215590A1 (en) |
WO (1) | WO2003070364A1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20100176287A1 (en) * | 2006-11-23 | 2010-07-15 | Koninklijke Philips Electronics N.V. | Device for separation and maldi analysis of an analyte in a sample |
CN109917060A (en) * | 2019-02-28 | 2019-06-21 | 云南中烟工业有限责任公司 | A method of utilizing tracer-Headspace Gas Chromatography paper oleophobic degree |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10340429A1 (en) * | 2003-09-02 | 2005-04-07 | GESELLSCHAFT FüR BIOTECHNOLOGISCHE FORSCHUNG MBH (GBF) | Hydrophobic object with a pattern of hydrophilic areas, its production and use |
DE102008059506A1 (en) | 2008-11-28 | 2010-06-10 | Qiagen Gmbh | Carrier plate useful as carrier of cell suspension during electroporation, comprises carrier surface having wetting areas that are wettable with cell suspension and rejection area that is not wettable with cell suspension, and electrode |
DE102015003440A1 (en) | 2015-03-16 | 2016-09-22 | Friedrich-Schiller-Universität Jena | Method for MALDI-MSI analysis of objects, in particular biological tissue samples, and target for analysis and its production |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5041266A (en) * | 1989-12-21 | 1991-08-20 | Hoffmann-La Roche Inc. | Tray for immunometric determinations |
US5674592A (en) * | 1995-05-04 | 1997-10-07 | Minnesota Mining And Manufacturing Company | Functionalized nanostructured films |
US5958345A (en) * | 1997-03-14 | 1999-09-28 | Moxtek, Inc. | Thin film sample support |
US6287872B1 (en) * | 1997-12-11 | 2001-09-11 | Bruker Daltonik Gmbh | Sample support plates for Maldi mass spectrometry including methods for manufacture of plates and application of sample |
US6462115B1 (en) * | 1996-08-16 | 2002-10-08 | Nippon Telegraph And Telephone Corporation | Water repellent coating composition, method for preparing the same, and coating films and coated articles using the same |
US20030108449A1 (en) * | 2000-02-09 | 2003-06-12 | Karsten Reihs | Ultraphobic surface structure having a plurality of hydrophilic areas |
US20050279927A1 (en) * | 2002-02-22 | 2005-12-22 | Joachim Engelking | Use of ultraphobic surfaces having a multitude of hydrophilic areas for analyzing samples |
US20060013735A1 (en) * | 2002-02-22 | 2006-01-19 | Joachim Engelking | Ultraphobic surface having a multitude of reversibly producible hydrophilic and/or oleophilic areas |
US7285331B1 (en) * | 1998-12-24 | 2007-10-23 | Qiagen Gmbh | Ultraphobic surface |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000039368A2 (en) * | 1998-12-24 | 2000-07-06 | Bayer Aktiengesellschaft | Method for producing an ultraphobic surface on an aluminium base |
DE19949735A1 (en) * | 1999-10-15 | 2001-05-10 | Bruker Daltonik Gmbh | Processing of samples in solutions with a defined small wall contact area |
DE19923761C1 (en) * | 1999-05-21 | 2001-02-08 | Bruker Daltonik Gmbh | Processing of sample molecules of liquids, involves making the sample droplets stand or suspend from lyophilic or lyophobic anchors on flat support surfaces |
DE10043042C2 (en) * | 2000-09-01 | 2003-04-17 | Bruker Daltonik Gmbh | Method for loading a sample carrier with biomolecules for mass spectrometric analysis |
-
2003
- 2003-02-24 WO PCT/EP2003/001860 patent/WO2003070364A1/en not_active Application Discontinuation
- 2003-02-24 US US10/505,617 patent/US20050282164A1/en not_active Abandoned
- 2003-02-24 AU AU2003215590A patent/AU2003215590A1/en not_active Abandoned
- 2003-02-24 EP EP03742575A patent/EP1478456A1/en not_active Withdrawn
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5041266A (en) * | 1989-12-21 | 1991-08-20 | Hoffmann-La Roche Inc. | Tray for immunometric determinations |
US5674592A (en) * | 1995-05-04 | 1997-10-07 | Minnesota Mining And Manufacturing Company | Functionalized nanostructured films |
US6462115B1 (en) * | 1996-08-16 | 2002-10-08 | Nippon Telegraph And Telephone Corporation | Water repellent coating composition, method for preparing the same, and coating films and coated articles using the same |
US5958345A (en) * | 1997-03-14 | 1999-09-28 | Moxtek, Inc. | Thin film sample support |
US6287872B1 (en) * | 1997-12-11 | 2001-09-11 | Bruker Daltonik Gmbh | Sample support plates for Maldi mass spectrometry including methods for manufacture of plates and application of sample |
US7285331B1 (en) * | 1998-12-24 | 2007-10-23 | Qiagen Gmbh | Ultraphobic surface |
US20030108449A1 (en) * | 2000-02-09 | 2003-06-12 | Karsten Reihs | Ultraphobic surface structure having a plurality of hydrophilic areas |
US20050279927A1 (en) * | 2002-02-22 | 2005-12-22 | Joachim Engelking | Use of ultraphobic surfaces having a multitude of hydrophilic areas for analyzing samples |
US20060013735A1 (en) * | 2002-02-22 | 2006-01-19 | Joachim Engelking | Ultraphobic surface having a multitude of reversibly producible hydrophilic and/or oleophilic areas |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20100176287A1 (en) * | 2006-11-23 | 2010-07-15 | Koninklijke Philips Electronics N.V. | Device for separation and maldi analysis of an analyte in a sample |
CN109917060A (en) * | 2019-02-28 | 2019-06-21 | 云南中烟工业有限责任公司 | A method of utilizing tracer-Headspace Gas Chromatography paper oleophobic degree |
Also Published As
Publication number | Publication date |
---|---|
WO2003070364A1 (en) | 2003-08-28 |
EP1478456A1 (en) | 2004-11-24 |
AU2003215590A1 (en) | 2003-09-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US7019288B2 (en) | Methods of making substrates for mass spectrometry analysis and related devices | |
Morozov et al. | Electrospray deposition as a method for mass fabrication of mono-and multicomponent microarrays of biological and biologically active substances | |
US7348183B2 (en) | Self-contained microelectrochemical bioassay platforms and methods | |
US6841379B2 (en) | Conductive microplate | |
US7399640B2 (en) | Structured biosample support plates for mass spectroscopic analyses and procedures for use | |
EP1208226B1 (en) | Immobilisation of unmodified nucleic acids to substrates having pendent acyl fluoride groups | |
US20070224688A1 (en) | Peptide or protein-capturing surfaces for high throughput MALDI mass spectrometry | |
US20050112650A1 (en) | Reactive polyurethane-based polymers | |
JP2002544508A5 (en) | ||
US20040029156A1 (en) | Immobilization of biomolecules on substrates by attaching them to adsorbed bridging biomolecules | |
US20060013735A1 (en) | Ultraphobic surface having a multitude of reversibly producible hydrophilic and/or oleophilic areas | |
WO2006037527A1 (en) | Masked solid porous supports allowing fast and easy reagent exchange to accelerate electrode-based microarrays | |
WO2005038048A1 (en) | Apparatus and methods for detecting nucleic acid in biological samples | |
US20050282164A1 (en) | Ultraphobic sample carrier having functional hydrophilic and/or oleophilic areas | |
US7456392B2 (en) | Use of ultraphobic surfaces having a multitude of hydrophilic areas for analyzing samples | |
JP4497903B2 (en) | Protein chip and biosensor using the same | |
JP4197279B2 (en) | Biologically-derived substance detection substrate and manufacturing method thereof | |
EP1907844B1 (en) | Method of manufacturing probe-immobilized carrier | |
JP4814098B2 (en) | Device and method for binding / desorbing targets or objects present in a sample | |
EP3566052A1 (en) | Ligand binding assays using maldi-tof mass spectrometry | |
US20030008159A1 (en) | Roll of web having reactive groups on its surface | |
JP2004163394A (en) | Liquid droplet discharging head and method for manufacturing the same, apparatus for manufacturing microarray, and method for manufacturing the microarray | |
US20030108878A1 (en) | DNA chip and its preparation | |
WO2002039113A2 (en) | Schiff base reductant co-dispense process | |
DE10207616A1 (en) | Planar structure comprises ultraphobic surface and hydrophilic or oleophilic regions having an additional functionality, useful as a carrier for e.g. binding agents |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: SUNYX SURFACE NANOTECHNOLOGIES GMBH, GERMANY Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:ENGELKING, JOACHIM;REIHS, KARSTEN;NORDHOFF, ECKHARD;AND OTHERS;REEL/FRAME:016872/0114;SIGNING DATES FROM 20050701 TO 20050721 |
|
AS | Assignment |
Owner name: QIAGEN GMBH, GERMANY Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:SUNYX SURFACE NANOTECHNOLOGIES GMBH;REEL/FRAME:017896/0831 Effective date: 20060314 |
|
STCB | Information on status: application discontinuation |
Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION |