US20020136736A1 - Papillomavirus L2 protein - Google Patents
Papillomavirus L2 protein Download PDFInfo
- Publication number
- US20020136736A1 US20020136736A1 US10/054,695 US5469502A US2002136736A1 US 20020136736 A1 US20020136736 A1 US 20020136736A1 US 5469502 A US5469502 A US 5469502A US 2002136736 A1 US2002136736 A1 US 2002136736A1
- Authority
- US
- United States
- Prior art keywords
- protein
- papillomavirus
- fragment
- formulation according
- bpv
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
- A61K2039/552—Veterinary vaccine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55511—Organic adjuvants
- A61K2039/55566—Emulsions, e.g. Freund's adjuvant, MF59
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/58—Medicinal preparations containing antigens or antibodies raising an immune response against a target which is not the antigen used for immunisation
- A61K2039/585—Medicinal preparations containing antigens or antibodies raising an immune response against a target which is not the antigen used for immunisation wherein the target is cancer
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/70—Multivalent vaccine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/20011—Papillomaviridae
- C12N2710/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/20011—Papillomaviridae
- C12N2710/20034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
Definitions
- the present invention relates to the use of papillomavirus L2 protein in medicine, particularly for the regression of papillomavirus tumours in mammals; and to pharmaceutical formulations comprising the L2 protein.
- Papillomaviruses induce a variety of lesions both in humans and in animals. Some papillomas, albeit benign, are themselves a clinical problem, such as laryngeal papillomas of children (Steinberg and Abramson, 1985) or penile papillomas of bulls (Jarrett, 1985a), and others are known to be a risk factor in the pathogenesis of cancer, as in the case of flat lesions of the cervix or penile condylomata in humans (zur Hausen, 1978). Therefore both in human and veterinary medicine an antiviral vaccine, particularly a therapeutic one inducing lesion rejection, would be of major importance.
- Vaccination studies in humans present several problems: first of all experimentation is ethically unacceptable and, secondly, very limited amounts of virus are available as some lesions, in particular those of the cervix, do not produce viral progeny, and no in vitro system is yet available which allows vegetative replication of virus.
- the production of viral proteins in bacteria and the use of synthetic peptides have circumvented this last problem and have allowed the ongoing analysis of the immune response to papillomavirus infection (see for instance Jenison et al, 1988: Jochmus-Kudielka et al, 1989; Tindle et al, 1990, Dillner, 1990 and Strang et al, 1990).
- the bovine system is an excellent model for the human one, given the several similarities between the two: multiple virus types with high lesion specificity (Campo et al, 1981; Jarrett et al, 1984), homology of genetic structure (Danos et al, 1984) and progression of some lesions to malignancy (Jarrett et al, 1978).
- the bovine system also presents several advantages: cofactors in oncogenesis are known (Jarrett et al, 1978; Campo and Jarrett, 1986) and, above all, direct experimentation is possible (Jarrett, 1985a).
- the present invention resides in the discovery that the papillomavirus L2 protein may be prophylactically or therapeutically effective in the treatment of papillomavirus tumours.
- the present invention provides the use of papillomavirus L2 protein in medicine, particularly for the prophylaxis or therapy of papillomavirus tumours.
- the invention also provides a pharmaceutical formulation for the prophylaxis or therapy of papillomavirus tumours, which comprises; papillomavirus L2 protein in admixture with a pharmaceutically acceptable carrier.
- the invention further provides papillomavirus L2 protein for use in the production of a medicament for use in medicine, particularly for use in the prophylaxis or therapy of papillomavirus tumours.
- the invention still further provides a method of treating a mammal for the prophylaxis or therapy of papillomavirus tumours, which comprises the administration of papillomavirus L2 protein to the mammal.
- the prophylactic or therapeutic effect of the L2 protein may be limited to the respective papillomavirus type.
- the therapy will be applicable to papillomavirus infections of mammals, including humans and animals.
- the invention is particularly applicable for the therapy and regression of laryngeal tumours, skin cancer tumours and genital lesions, whether malignant or not.
- the therapy is particularly useful for the regression of tumours on animals, for example the removal of warts from the udders of milk cows, or removal of papillomas of the alimentary canal and for the treatment of horses and donkeys.
- Prophylactic vaccination may also be employed.
- the L2 protein is generally produced by recombinant DNA techniques.
- a plasmid containing the gene coding for the L2 protein may be transfected into E. coli and cultured.
- the entire L2 protein as it exists in nature may be employed, or a fragment (such as amino acid 90 to 467 of BPV-2 as disclosed hereafter) or fragments thereof may be used providing that the therapeutic effectiveness is retained.
- the L2 protein may be the native form, with additions, deletions or substitutions which do not substantially effect its therapeutic effectiveness.
- the L2 protein will usually be administered in the form of a pharmaceutical formulation.
- the formulation contains a pharmaceutically acceptable carrier.
- the carrier must be acceptable in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
- the pharmaceutical formulation is preferably formulated for parenteral administration, including subcutaneous, intramuscular and intravenous injection; or as a suppository or pessary.
- parenteral administration the formulation may be presented as a sterile solution or suspension in a suitable liquid vehicle, which may also contain preservatives and materials for rendering the formulation isotonic.
- the formulations may be presented in unit-dose or multi-dose containers.
- the carrier will generally be apyrogenic. Each dose will generally contain 100 to 10,000 micro grams of the L2 protein.
- the protein may be administered together with an adjuvant, such as Freund's incomplete adjuvant, as an oil-in-water emulsion or using other adjuvant systems known in the art such as L101 and DDA as used in Pilacinski et al. (1986).
- an adjuvant such as Freund's incomplete adjuvant, as an oil-in-water emulsion or using other adjuvant systems known in the art such as L101 and DDA as used in Pilacinski et al. (1986).
- FIG. 1 shows the open reading frames for L2 protein of BPV-2
- FIGS. 2, 3 and 4 show the results of vaccination experiments using L2 protein of BPV-4 for control group, L2 vaccinated group, and L2 plus E7 vaccinated group respectively.
- FIG. 1 referred to in the experimental protocol shows the L1 and L2 open reading frames (ORF's) of BPV2 and the restriction enzyme sites used for cloning.
- T TATA box
- A polyadenylation site
- Met translation initiation codon
- TAA translational termination codon
- B BamHI site
- Hp HpaI site
- H HindIII site.
- the DNA fragment cloned in pUR is indicated as L2 (BamHI-BamHI). The nucleotide numbering of Potter and Meinke (1985) is used.
- Friesian calves Twenty one 12-week old male Friesian calves were obtained from a papilloma-free source. They were randomly assigned to three initial groups, which were housed in separate, clean, well ventilated pens in the isolation unit of the Department of Veterinary Pathology, Glasgow. All the calves were bled on arrival for haematological analysis and to obtain pre-inoculation serum samples. The experiment was started when the calves were 16 weeks old.
- ORF open reading frame encoding the L2 peptide was isolated by digesting the BPV-2 genome cloned in pAT 153 (Campo and Coggins, 1982) with Bam HI. This produced one fragment of 2030 bp (nt 268-2298) numbered according to the nucleotide (nt) sequence of Potter and Meinke (1985), where nt 1 is the A of the ATG codon of the L2 ORF (FIG. 1); this fragment contains the majority of the L2 ORF (from aa 90 to aa 467, L2), the L2 ORF stop codon and the 5′ half of the L1 ORF, which would not be expressed because of the termination codon.
- Peptide for vaccination was prepared from mid-log phase cultures induced for 4 hours in L-broth supplemented with 100 ug/ml ampicillin and containing 1 mM IPTG. Bacterial pellets resuspended in lysozyme buffer (50 mM TRIS-HC1 pH 8.0, 10 mM McCl 2, 50 mM glucose, 1 mg/ml lysozyme) were left at 20° C. for 10 min, when EDTA was added to 50 mM.
- lysozyme buffer 50 mM TRIS-HC1 pH 8.0, 10 mM McCl 2, 50 mM glucose, 1 mg/ml lysozyme
- the fusion peptide was pelleted at 39000 g for 30 min and resuspended by boiling and sonication in 5% SDS, 50 mM B-mercaptoethanol, 50 mM TRIS-HCl, pH 8.0 Purity of 90-95% was achieved by preparative SDS PAGE, the final yields being up to 2 mg of product per gm wet weight of cells.
- the protein was stored at ⁇ 20° C. before use, but prolonged storage caused degradation.
- the vaccination experiments were designed as follows: In group A, six animals were vaccinated prophylactically with the gel-purified L2 (one calf had to be withdrawn from the experiment because of pneumonia); three of these animals were also vaccinated therapeutically with the gel-purified L2 nine weeks after callenge. In group B, eleven animals received no prophylactic vaccination; after tumor formation three of these animals were therapeutically vaccinated with gel-purified L2, while eight animals received no vaccine at all and were therefore used as controls.
- the calves receiving the L2 vaccine were given a 1 ml PBS suspension containing 650 ug of the L2 fusion protein plus 1 ml of Freund's incomplete adjuvant (FIA) into the right quadriceps muscle. This was repeated fourteen days later as a boost, but with only 500 g of protein.
- FIA Freund's incomplete adjuvant
- BPV-2 was purified from a skin fibropapilloma (Campo et al, 1981) and the concentration of viral particles was estimated by the electron microscope assay (Jarrett et al, 1990a). Each calf was challenged at multiple sites with 10 12 virus particles as described by Jarrett and other (1990a) either four weeks after vaccination (two weeks after the boost) or nine weeks before vaccination.
- Biopsies were performed as described by Jarrett et al (1990a). Immunocytochemical studies were made by the peroxidase-anti-peroxidase (Hsu et al, 1981) or immunogold (Holgate et al, 1983) techniques using rabbit anti-BPV-2 serum as described by Jarrett et al (1984).
- the size of the BPV-2 B-gal-L2 fusion protein was estimated on PAGE to be 180 kDa well in agreement with the predicted size of 156 kDa.
- the L2 fusion protein was characterized immunologically. It was injected into rabbits or calves and the antisera were tested against the fusion protein itself and against virion proteins in both Ouchterlony and Western blots assays. The antisera were reactive with both the engineered protein (data not shown) and its viral L2 (62 kDA) counterpart. In reciprocal experiments, rabbit or calf antisera raised against SDS-disrupted virus were reactive with the fusion protein. Although N-terminus truncated, the fusion protein therefore shares epitopes with virus and presents them effectively to the host immune system.
- Vaccination with the L2 fusion protein induced early tumor regression. Tumor regression was accompanied by infiltration of the lesion by macrophages and lymphocytes, a process consistently observed when natural regression takes place (Jarrett, 1985a). Thus it appears that the L2 protein encodes epitopes specific for the cellular effector arm of the immune system. Zhou et al (1991) have recently shown that the L1 protein of HPV-16, when expressed in vaccinia virus, induces cytotoxic T-lymphocytes in infected mice, providing another example of T-cell activation by a structural protein.
- L2 open reading frame (ORF) of BPV-4 was cloned following the general procedure of Example 1, except that plasmid pGEX was employed which resulted in a L2 fusion protein with glutathione S-transferase (GST) as coprotein.
- the L2 ORF was cloned as the whole ORF (encoding amino acids 8 to 525) and as the three fragments encoding amino acids 11-201, 203-329, and 330-525. In the subsequent vaccination experiments a mixture of these four was used. Expression was in E. coli and the proteins were purified by gel chromatography, as before.
- the E7 protein was prepared in analogous manner.
- Vaccination was carried out as in Example 1 using Freund's Incomplete Adjuvant, except that doses of 1 mg total protein (L2 and fragments) was administered both as the dose (day 0) and the booster (day 28).
- Group 1 was vaccinated with L2 vaccine alone.
- Group 2 was vaccinated with L2 plus E7 vaccine.
- Group 3 was the control non-vaccinated group.
- FIG. 2 controls
- FIG. 3 L2 alone
- FIG. 3 L2 plus E7
- the controls showed a good tumor response, 13 of the 17 animals being infected.
- L2 vaccinated group only one animal showed a response (a small plaque).
- L2 plus E7 only one animal developed tumours.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Epidemiology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mobile Radio Communication Systems (AREA)
- Transceivers (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The L2 protein of papillomavirus (particularly BPV-2 and BPV-4) has been cloned as a fusion protein with beta-galactosi-dase and GST; both as the whole protein and as fragments. Vaccination of calves is found to have both a prophylactic effect in tumor prevention and a therapeutic effect in tumor regression.
Description
- The present invention relates to the use of papillomavirus L2 protein in medicine, particularly for the regression of papillomavirus tumours in mammals; and to pharmaceutical formulations comprising the L2 protein.
- Papillomaviruses induce a variety of lesions both in humans and in animals. Some papillomas, albeit benign, are themselves a clinical problem, such as laryngeal papillomas of children (Steinberg and Abramson, 1985) or penile papillomas of bulls (Jarrett, 1985a), and others are known to be a risk factor in the pathogenesis of cancer, as in the case of flat lesions of the cervix or penile condylomata in humans (zur Hausen, 1978). Therefore both in human and veterinary medicine an antiviral vaccine, particularly a therapeutic one inducing lesion rejection, would be of major importance. Vaccination studies in humans present several problems: first of all experimentation is ethically unacceptable and, secondly, very limited amounts of virus are available as some lesions, in particular those of the cervix, do not produce viral progeny, and no in vitro system is yet available which allows vegetative replication of virus. The production of viral proteins in bacteria and the use of synthetic peptides have circumvented this last problem and have allowed the ongoing analysis of the immune response to papillomavirus infection (see for instance Jenison et al, 1988: Jochmus-Kudielka et al, 1989; Tindle et al, 1990, Dillner, 1990 and Strang et al, 1990). Whilst investigation into the feasibility of a human papillomavirus vaccine is still at an early stage, effective prophylactic vaccines, both natural (Jarrett et al, 1990a) and genetically engineered (Pilachinski et al, 1986) have already been produced against bovine papillomaviruses, and regression of Shope papillomas has been achieved by vaccinating rabbits with tumor tissue extracts (Evans et al, 1962). The bovine system is an excellent model for the human one, given the several similarities between the two: multiple virus types with high lesion specificity (Campo et al, 1981; Jarrett et al, 1984), homology of genetic structure (Danos et al, 1984) and progression of some lesions to malignancy (Jarrett et al, 1978). The bovine system also presents several advantages: cofactors in oncogenesis are known (Jarrett et al, 1978; Campo and Jarrett, 1986) and, above all, direct experimentation is possible (Jarrett, 1985a).
- It has recently been shown that vaccination with bovine papillomavirus type 2 (BPV-2) successfully prevented infection by the same virus (Jarrett et al, 1990a), but not by other virus types (Jarrett et al, 1990b). Prevention was accompanied by production of neutralising antibodies in the serum of vaccinated animals, indicating that neutralising epitopes are present in the virus.
- Generally speaking, the present invention resides in the discovery that the papillomavirus L2 protein may be prophylactically or therapeutically effective in the treatment of papillomavirus tumours.
- Thus, the present invention provides the use of papillomavirus L2 protein in medicine, particularly for the prophylaxis or therapy of papillomavirus tumours.
- The invention also provides a pharmaceutical formulation for the prophylaxis or therapy of papillomavirus tumours, which comprises; papillomavirus L2 protein in admixture with a pharmaceutically acceptable carrier.
- The invention further provides papillomavirus L2 protein for use in the production of a medicament for use in medicine, particularly for use in the prophylaxis or therapy of papillomavirus tumours.
- The invention still further provides a method of treating a mammal for the prophylaxis or therapy of papillomavirus tumours, which comprises the administration of papillomavirus L2 protein to the mammal.
- Generally speaking, the prophylactic or therapeutic effect of the L2 protein may be limited to the respective papillomavirus type. Thus, for general therapeutic applications, especially where the particular papillomavirus type is unknown, it may be desirable to employ a mixture of L2 proteins from a variety of papillomavirus types.
- Generally, the therapy will be applicable to papillomavirus infections of mammals, including humans and animals. In humans, the invention is particularly applicable for the therapy and regression of laryngeal tumours, skin cancer tumours and genital lesions, whether malignant or not. In animals, the therapy is particularly useful for the regression of tumours on animals, for example the removal of warts from the udders of milk cows, or removal of papillomas of the alimentary canal and for the treatment of horses and donkeys. Prophylactic vaccination may also be employed.
- The L2 protein is generally produced by recombinant DNA techniques. In particular, a plasmid containing the gene coding for the L2 protein may be transfected intoE. coli and cultured. The entire L2 protein as it exists in nature may be employed, or a fragment (such as amino acid 90 to 467 of BPV-2 as disclosed hereafter) or fragments thereof may be used providing that the therapeutic effectiveness is retained. The L2 protein may be the native form, with additions, deletions or substitutions which do not substantially effect its therapeutic effectiveness.
- The L2 protein will usually be administered in the form of a pharmaceutical formulation. The formulation contains a pharmaceutically acceptable carrier. The carrier must be acceptable in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
- Since the protein is broken down in the stomach, oral administration is not preferred. The pharmaceutical formulation is preferably formulated for parenteral administration, including subcutaneous, intramuscular and intravenous injection; or as a suppository or pessary. For parenteral administration the formulation may be presented as a sterile solution or suspension in a suitable liquid vehicle, which may also contain preservatives and materials for rendering the formulation isotonic. The formulations may be presented in unit-dose or multi-dose containers. The carrier will generally be apyrogenic. Each dose will generally contain 100 to 10,000 micro grams of the L2 protein.
- In order to enhance the therapeutic effect of the protein, it may be administered together with an adjuvant, such as Freund's incomplete adjuvant, as an oil-in-water emulsion or using other adjuvant systems known in the art such as L101 and DDA as used in Pilacinski et al. (1986).
- Embodiments of the present invention will now be described by way of example only with reference to the following experimental protocol.
- FIG. 1 shows the open reading frames for L2 protein of BPV-2;
- FIGS. 2, 3 and4 show the results of vaccination experiments using L2 protein of BPV-4 for control group, L2 vaccinated group, and L2 plus E7 vaccinated group respectively.
- FIG. 1 referred to in the experimental protocol shows the L1 and L2 open reading frames (ORF's) of BPV2 and the restriction enzyme sites used for cloning.
- T=TATA box; A=polyadenylation site; Met=translation initiation codon; TAA=translational termination codon; B=BamHI site; Hp=HpaI site; H=HindIII site. The DNA fragment cloned in pUR is indicated as L2 (BamHI-BamHI). The nucleotide numbering of Potter and Meinke (1985) is used.
- Twenty one 12-week old male Friesian calves were obtained from a papilloma-free source. They were randomly assigned to three initial groups, which were housed in separate, clean, well ventilated pens in the isolation unit of the Department of Veterinary Pathology, Glasgow. All the calves were bled on arrival for haematological analysis and to obtain pre-inoculation serum samples. The experiment was started when the calves were 16 weeks old.
- The open reading frame (ORF) encoding the L2 peptide was isolated by digesting the BPV-2 genome cloned in pAT 153 (Campo and Coggins, 1982) with Bam HI. This produced one fragment of 2030 bp (nt 268-2298) numbered according to the nucleotide (nt) sequence of Potter and Meinke (1985), where
nt 1 is the A of the ATG codon of the L2 ORF (FIG. 1); this fragment contains the majority of the L2 ORF (from aa 90 to aa 467, L2), the L2 ORF stop codon and the 5′ half of the L1 ORF, which would not be expressed because of the termination codon. The fragment was cloned in the pUR vector series (Ruther and Muller-Hill, 1983), giving rise to pL2, and transfected into E. Coli JM 109. Peptide for vaccination was prepared from mid-log phase cultures induced for 4 hours in L-broth supplemented with 100 ug/ml ampicillin and containing 1 mM IPTG. Bacterial pellets resuspended in lysozyme buffer (50 mM TRIS-HC1 pH 8.0, 10 mM McCl2, 50 mM glucose, 1 mg/ml lysozyme) were left at 20° C. for 10 min, when EDTA was added to 50 mM. Following cell lysis by the addition of Triton X100 to 1% (v/v), the fusion peptide was pelleted at 39000 g for 30 min and resuspended by boiling and sonication in 5% SDS, 50 mM B-mercaptoethanol, 50 mM TRIS-HCl, pH 8.0 Purity of 90-95% was achieved by preparative SDS PAGE, the final yields being up to 2 mg of product per gm wet weight of cells. The protein was stored at −20° C. before use, but prolonged storage caused degradation. - The vaccination experiments were designed as follows: In group A, six animals were vaccinated prophylactically with the gel-purified L2 (one calf had to be withdrawn from the experiment because of pneumonia); three of these animals were also vaccinated therapeutically with the gel-purified L2 nine weeks after callenge. In group B, eleven animals received no prophylactic vaccination; after tumor formation three of these animals were therapeutically vaccinated with gel-purified L2, while eight animals received no vaccine at all and were therefore used as controls.
- The calves receiving the L2 vaccine were given a 1 ml PBS suspension containing 650 ug of the L2 fusion protein plus 1 ml of Freund's incomplete adjuvant (FIA) into the right quadriceps muscle. This was repeated fourteen days later as a boost, but with only 500 g of protein.
- BPV-2 was purified from a skin fibropapilloma (Campo et al, 1981) and the concentration of viral particles was estimated by the electron microscope assay (Jarrett et al, 1990a). Each calf was challenged at multiple sites with 1012 virus particles as described by Jarrett and other (1990a) either four weeks after vaccination (two weeks after the boost) or nine weeks before vaccination.
- Biopsies were performed as described by Jarrett et al (1990a). Immunocytochemical studies were made by the peroxidase-anti-peroxidase (Hsu et al, 1981) or immunogold (Holgate et al, 1983) techniques using rabbit anti-BPV-2 serum as described by Jarrett et al (1984).
- The presence of neutralizing antibodies in serum samples was determined by the cell transformation inhibition assay described previously (Jarrett et al, 1990a). This assay takes advantage of the ability of BPV-2 to transform primary bovine fibroblasts in vitro (Jarrett, 1985b), which is abrogated by pre-incubation of virus with immune serum.
- The size of the BPV-2 B-gal-L2 fusion protein was estimated on PAGE to be 180 kDa well in agreement with the predicted size of 156 kDa.
- The L2 fusion protein was characterized immunologically. It was injected into rabbits or calves and the antisera were tested against the fusion protein itself and against virion proteins in both Ouchterlony and Western blots assays. The antisera were reactive with both the engineered protein (data not shown) and its viral L2 (62 kDA) counterpart. In reciprocal experiments, rabbit or calf antisera raised against SDS-disrupted virus were reactive with the fusion protein. Although N-terminus truncated, the fusion protein therefore shares epitopes with virus and presents them effectively to the host immune system.
- Five animals were vaccinated prophylactically; three of these and three unvaccinated animals were vaccinated therapeutically nine weeks after challenge. As the same results were obtained with the two groups of calves, they will be considered together. All animals developed fibropapillomas four weeks after challenge (Table 1). Six vaccinated animals were still bearing tumours at ten weeks. In the other two vaccinated calves the tumours were entering the rejection phase: the epithelium was virtually normal and the sub-epithelial tissue was mainly composed of hyalinised collagen. There was a drastic reduction in the number of fibroblasts and a massive infiltration of lymphocytes and macrophages in the sub-epithelial tissue. All vaccinated animals had reached that stage by week thirteen. By week sixteen the tumours had definitely regressed. There were small plaque-like lesions with hyperkeratosis, but virtually all the normal skin adnexal elements were present. Some lymphocytes and macrophages were still present. The control animals were still bearing virus-producing tumours (Table 1). Neutralizing antibodies appeared in the serum of the vaccinated calves at the same time and with the same titre as the control animals (data not shown). Serum antibodies to L2 were however detected soon after vaccination and before challenge (data not shown).
- Vaccination with the L2 fusion protein, whether delivered before or after challenge, induced early tumor regression. Tumor regression was accompanied by infiltration of the lesion by macrophages and lymphocytes, a process consistently observed when natural regression takes place (Jarrett, 1985a). Thus it appears that the L2 protein encodes epitopes specific for the cellular effector arm of the immune system. Zhou et al (1991) have recently shown that the L1 protein of HPV-16, when expressed in vaccinia virus, induces cytotoxic T-lymphocytes in infected mice, providing another example of T-cell activation by a structural protein.
- In field and experimental cases, rejection takes place approximately twelve months after infection and it generally follows ulceration of the lesion. This is consistent with the L2 being internal to the virion (Jin et al, 1989) and therefore not readily exposed to the host immune system; ulceration of the tumor with associated bleeding would lead to the exposure of relatively large amounts of antigen to the immune cells.
- Anti L2 antibodies were present in the serum of the vaccinated animals, but these had no activity in the neutralization assay. Therefore, unless some neutralizing epitopes are present in the first N-terminus amino acids of L2, which are missing in our fusion protein, it is unlikely that L2 plays a significant role in conferring prophylactic protection.
TABLE 1 Effect of vaccination with BPV-2 L2 fusion proteins. WEEKS POST CHALLENGE 4 7 10 13 16 CONTROLS 1 FP FP FP nd nd 2 FP FP FP nd nd 3 FP FP FP nd nd 4 FP FP FP nd nd 5 FP FP FP nd nd 6 FP FP FP nd nd 7 FP nd FP FP FP 8 FP nd FP FP FP L2 VACCINATES (bc) 13* FP nd FP — — 14 FP nd — — — 15* FP nd FP — — 16 FP nd — — — 17* FP nd FP — — L2 VACCINATES (ac) 18 FP nd FP — — 19 FP nd FP — — 20 FP nd FP — — - L2 open reading frame (ORF) of BPV-4 was cloned following the general procedure of Example 1, except that plasmid pGEX was employed which resulted in a L2 fusion protein with glutathione S-transferase (GST) as coprotein. The L2 ORF was cloned as the whole ORF (encoding
amino acids 8 to 525) and as the three fragments encoding amino acids 11-201, 203-329, and 330-525. In the subsequent vaccination experiments a mixture of these four was used. Expression was in E. coli and the proteins were purified by gel chromatography, as before. - The E7 protein was prepared in analogous manner.
- Vaccination was carried out as in Example 1 using Freund's Incomplete Adjuvant, except that doses of 1 mg total protein (L2 and fragments) was administered both as the dose (day 0) and the booster (day 28).
- 47 calves, of about 10 weeks of age at the start of the experiment, were housed in an isolation compound. They were divided into 2 groups of 15 and one of 17 (controls).
-
Group 1 was vaccinated with L2 vaccine alone. -
Group 2 was vaccinated with L2 plus E7 vaccine. - Group 3 was the control non-vaccinated group.
- All animals were examined and bled before
Day 0. They were vaccinated onDay 0 and Day 28. They were challenged with BPV-4 virus onDay 43 and examined fortumor formation 4 and 7 weeks later. - The results are shown in FIG. 2 (controls), FIG. 3 (L2 alone) and FIG. 3 (L2 plus E7). The controls showed a good tumor response, 13 of the 17 animals being infected. In the L2 vaccinated group only one animal showed a response (a small plaque). In the group vaccinated with L2 plus E7 only one animal developed tumours.
- Thus the L2 protein of BPV-4 appears to be exerting a strong prophylactic effect in preventing tumor formation (in contrast to BPV-2 where a therapeutic effect was exhibited).
- Campo M S and Coggins L W (1982) Molecular cloning of bovine papillomavirus genomes and comparison of their sequence homologies by heteroduplex mapping. Journal General Virology, 63, 255-264.
- Campo M S and Jarrett W F H (1986) Papillomavirus infection in cattle: viral and chemical cofactors in naturally occurring and experimentally induced tumours. Ciba Foundation Symposium 120; Papillomaviruses. pp 117-131.
- Campo M S, Moar M H, Laird H M and Jarrett W F H (1981) Molecular heterogeneity and lesion site specificity of cutaneous bovine papillomaviruses. Virology, 113, 323-335.
- Cowsert L M, Lake P and A B Jenson (1987) Topographical and conformational epitopes of
bovine papillomavirus type 1 defined by monoclonal antibodies. Journal of the National Cancer Institute, 79, 1053-1057. - Danos O, Giri I, Thierry F and Yaniv M (1984) Papillomavirus Genomes: Sequences and Consequences. The Journal of Investigative Dermatology, 83, 7s-11s.
- Dillner J (1990) Mapping of linear epitopes of human papillomavirus type 16: the E1, E2, E4, E5, E6 and E7 open reading frames. International Journal of Cancer, 46, 703-711.
- Dvoretzky I, Shober R, Chattopadhyay S K and Lowy D R (1980) A quantitative in vitro focus forming assay for bovine papillomavirus. Virology, 103, 369-375.
- Evans C A, Gorman L R, Ito Y and Weiser R S (1962) Antitumour immunity in the Shope papilloma-carcinoma complex of rabbits. I. Papilloma regression induced by homologous and autologous tissue vaccines. Journal of the National Cancer Institute, 29, 277-285.
- Holgate C S, Jackson P, Cowen P N and Berol C C (1983) Immunogold silver staining: a new method of immunostaining with enhanced sensitivity. Journal of histochemistry and cytochemistry, 31, 938-944.
- Hsu S M, Raine L and Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques. Journal of histochemistry and cytochemistry, 29, 577-580.
- Jaggar R T, Pennie W D, Smith K T, Jackson M E and Campo M S (1990) Cooperation between
bovine papillomavirus type 4 and ras in the morphological transformation of primary bovine fibroblasts. Journal of General Virology, 71, 3041-3046. - Jarrett W F H (1985a) The natural history of bovine papillomavirus infection. Advances in viral oncology. Ed: G. Klein. vol 5, pp83-102.
- Jarrett W F H (1985b) Biological characteristics of bovine papillomaviruses. UCLA Symposium. Ed P M Howley and T R Broker. vol 32: Papillomaviruses; Molecular and clinical aspects. pp 299-303.
- Jarrett W F H, McNeil P E, Grimshaw W T R, Selman I E and McIntyre W I M (1978) High incidence area of cattle cancer with a possible interaction between an environmental carcinogen and a papillomavirus. Nature, 274, 215-217.
- Jarrett W F H, Campo M S, O'Neil B W, Laird H M and Coggins L W (1984) A novel bovine papillomavirus (BPV-6) causing true epithelial Papillomas of the mammary gland skin: a member of a proposed new subgroup. Virology, 136, 255-264.
- Jarrett W F H, O'Neil B W, Gaukroger J M, Laird H M, Smith K T and Campo M S (1990a) Studies on vaccination against papillomaviruses: a comparison of purified virus, tumor extract and transformed cells in prophylactic vaccination. The Veterinary Record, 126, 449-452.
- Jarrett W F H, O'Neil B W, Gaukroger J M, Smith K T, Laird H M and Campo M S (1990b) Studies an vaccination against papillomaviruses:the immunity after infection and vaccination with bovine papillomaviruses of different types. The veterinary Record, 126, 473-475.
- Jenison S A, Firzlaff J M, Langenberg A and Galloway D A (1988) Identification of immunoreactive antigens of human papillomavirus type 6b by usingEscherichia coli-expressed fusion proteins. Journal of Virology, 62, 2115-2123.
- Jin X W, Cowsert L M, Pilacinski W P and Jenson A B (1989) Identification of L2 open reading frame gene products of
bovine papillomavirus type 1 using monoclonal antibodies. Journal of General Virology, 70, 1133-1140. - Jin X W, Cowsert L M, Marshall D, Reed D, Pilacinski W P, Lim L and Jenson A B (1990) Bovine serological response to a recombinant BPV-1 major capsid protein vaccine. Intervirology, 31, 345-354.
- Jochmus-Kudielka I, Schneider A, Braun R, Kimmig R, Koldovsky U, Schneweis K E, Seerdof K and Gissmann L (1989) Journal of the National Cancer Institute, 81, 1698-1704.
- Kreider J A, Howett M K, Wolfe S A, Bartlett G L, Zaino R J, Sedlacek T V and Mortel R (1985) Morphological transformation in vivo of human uterine cervix with papillomavirus from condylomata acuminata. Nature, 317, 639-640.
- Meneguzzi M, Cerni C, Kieny M P Lathe R (1991) Immunization against Human Papillomavirus type 16 tumor cells with recombinant vaccinia viruses expressing E6 and E7. Virology, 181, 62-69.
- Pilacinski W P, Glassman D L, Glassman K F, Reed D E, Lum M A, Marshall R F, Muscoplat C C and Faras A J (1986) Immunization against bovine papillomavirus infection. Ciba foundation Symposium 120; Papillomaviruses. pp 36-148.
- Potter H L and Meinke W J (1985) Nucleotide sequence of
bovine papillomavirus type 2 late region. Journal general Virology, 66, 187-193. - Ruther U and Muller-Hill B (1983) Easy identification of cDNA clones. EMBO Journal, 2, 1791-1794.
- Steinberg B M and Abramson A L (1985) Laryngeal papillomas. Clinics in Dermatology, 3, 130-138.
- Strang G, Hickling J K, McIndoe G A J, Howland K, Wilkinson D, Ikeda H and Rothbard J B. (1990) Human T-cell responses to human papillomavirus type 16 L1 and E6 synthetic peptides: identification of T-cell determinants, HLA-DR restriction and virus type specificity. Journal General Virology, 71, 423-431.
- Tindle R W, Smith J A, Geysen H m, Selvey L A and Frazer I H (1990) Identification of B epitopes in human papillomavirus type 16 E7 open reading frame protein. Journal General Virology, 71, 1347-1354.
- Zhou J, McIndoe A, Davies H, Sun X-Y and Crawford L (1991) The induction of cytotoxic T-lymonocyte precursor cells by recombinant vaccinia virus expressing human papillomavirus type 16 L1. Virology, 181, 203-210.
- zur Hausen H (1976) Condyloma acuminata and human genital cancer. Cancer Research, 36, 794.
Claims (15)
1. A pharmaceutical formulation for the prophylaxis or therapy of papillomavirus tumours, which comprises papillomavirus L2 protein or prophylactically or therapeutically effective fragment thereof in admixture with a pharmaceutically acceptable carrier.
2. A formulation according to claim 1 wherein the L2 protein is a bovine papillomavirus BPV-2 protein or fragment thereof.
3. A formulation according to claim 2 wherein the L2 protein fragment comprises substantially amino acids 90 to 467.
4. A formulation according to claim 1 wherein the L2 protein is a bovine papillomavirus BPV-4 protein or fragment thereof.
5. A formulation according to claim 1 in the form of an injectable formulation, wherein the carrier is a pharmaceutically acceptable injection vehicle.
6. A formulation according to claim 5 which further comprises an adjuvant.
7. A formulation according to claim 1 wherein the L2 protein is present in the form of a fusion protein with a different co-protein.
8. A formulation according to claim 7 wherein the co-protein in the L2 fusion protein is beta-galactosidase.
9. A formulation ac cording to claim 7 wherein the co-protein is glutathione S-transferase (GST).
10. A formulation according to claim 1 which further comprises papillomavirus E7 protein or effective fragment thereof.
11. A formulation according to claim 1 wherein the L2 protein or fragment thereof is produced by recombinant DNA techniques.
12. A transformed bacterial cell producing recombinant L2 protein or therapeutically effective fragment thereof.
13. Use of papillomavirus L2 protein or effective fragment thereof in medicine for the prophylaxis or therapy of papillomavirus tumours.
14. Use of papillomavirus L2 protein or therapeutically effective fragment thereof in the production of a medicament for use in the prophylaxis or therapy of papillomavirus tumours.
15. A method of treating a mammal for the therapy of papillomavirus tumours, which comprises the administration of papillomavirus L2 protein or effective fragment thereof to the mammal in a prophylactically or therapeutically effective dosage.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/054,695 US20020136736A1 (en) | 1991-06-26 | 2002-01-18 | Papillomavirus L2 protein |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9113809.9 | 1991-06-26 | ||
GB9113809A GB9113809D0 (en) | 1991-06-26 | 1991-06-26 | Papillomavirus l2 protein |
US08/484,791 US6380157B1 (en) | 1991-06-26 | 1995-06-07 | Method of treatment using papillomavirus L2 protein |
US10/054,695 US20020136736A1 (en) | 1991-06-26 | 2002-01-18 | Papillomavirus L2 protein |
Related Parent Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB1992/001092 Division WO1993000436A1 (en) | 1991-06-26 | 1992-06-17 | Papillomavirus l2 protein |
US08/484,791 Division US6380157B1 (en) | 1991-06-26 | 1995-06-07 | Method of treatment using papillomavirus L2 protein |
Publications (1)
Publication Number | Publication Date |
---|---|
US20020136736A1 true US20020136736A1 (en) | 2002-09-26 |
Family
ID=10697369
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US08/484,791 Expired - Lifetime US6380157B1 (en) | 1991-06-26 | 1995-06-07 | Method of treatment using papillomavirus L2 protein |
US10/054,695 Abandoned US20020136736A1 (en) | 1991-06-26 | 2002-01-18 | Papillomavirus L2 protein |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US08/484,791 Expired - Lifetime US6380157B1 (en) | 1991-06-26 | 1995-06-07 | Method of treatment using papillomavirus L2 protein |
Country Status (12)
Country | Link |
---|---|
US (2) | US6380157B1 (en) |
EP (1) | EP0592480B1 (en) |
JP (1) | JP3533216B2 (en) |
AT (1) | ATE179458T1 (en) |
AU (1) | AU662910B2 (en) |
DE (1) | DE69229050T2 (en) |
DK (1) | DK0592480T3 (en) |
ES (1) | ES2133322T3 (en) |
GB (1) | GB9113809D0 (en) |
GR (1) | GR3030716T3 (en) |
HK (1) | HK1001309A1 (en) |
WO (1) | WO1993000436A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011100234A2 (en) * | 2010-02-09 | 2011-08-18 | Stc.Unm | Immunogenic hpv l2-containing vlps and related compositions, constructs, and therapeutic methods |
WO2013106525A1 (en) * | 2012-01-12 | 2013-07-18 | Stc.Unm | Immunogenic hpv l2-containing vlps and related compositions and methods |
US9717783B2 (en) | 2010-02-09 | 2017-08-01 | Stc.Unm | Immunogenic HPV L2-containing VLPs and related compositions, constructs, and therapeutic methods |
Families Citing this family (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9207701D0 (en) * | 1992-04-08 | 1992-05-27 | Cancer Res Campaign Tech | Papillomavirus e7 protein |
US5618536A (en) * | 1992-09-03 | 1997-04-08 | The United States Of America As Represented By The Department Of Health And Human Services | Chimeric papillomavirus-like particles |
US20020164350A1 (en) | 1992-09-03 | 2002-11-07 | Lowy Douglas R. | Chimeric papillomavirus-like particles |
GB9306731D0 (en) * | 1993-03-31 | 1993-05-26 | Cancer Res Campaign Tech | Vaccines |
AU719837B2 (en) * | 1993-03-31 | 2000-05-18 | Cancer Research Campaign Technology Limited | Pharmaceuticals based on papillomaviruses |
AUPM358894A0 (en) * | 1994-01-31 | 1994-02-24 | Csl Limited | Modified papilloma virus l2 protein and vlps formed therefrom |
AU717647B2 (en) * | 1994-10-06 | 2000-03-30 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Chimeric papillomavirus-like particles |
GB9420146D0 (en) * | 1994-10-06 | 1994-11-23 | Cancer Res Campaign Tech | Papillomavirus vaccine |
AU717932B2 (en) * | 1994-10-06 | 2000-04-06 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Chimeric papillomavirus-like particles |
PT809700E (en) * | 1994-10-07 | 2006-09-29 | Univ Loyola Chicago | PARTICLES SIMILAR TO PAPILLOMAVIRUS AND FUSEOUS PROTEINS, AND METHODS FOR THEIR PRODUCTION |
AU4727296A (en) * | 1995-02-24 | 1996-09-11 | Cantab Pharmaceuticals Research Limited | Polypeptides useful as immunotherapeutic agents and methods of polypeptide preparation |
GB9505784D0 (en) * | 1995-03-22 | 1995-05-10 | Lynxvale Ltd | Anti-tumour treatment |
AU781653B2 (en) * | 1996-07-30 | 2005-06-02 | Transgene S.A. | Pharmaceutical composition for treating papollomavirus tumour and infection |
FR2751879B1 (en) * | 1996-07-30 | 1998-10-30 | Transgene Sa | PHARMACEUTICAL COMPOSITION AGAINST TUMORS AND PAPILLOMAVIRUS INFECTIONS |
US7118754B1 (en) | 1996-07-30 | 2006-10-10 | Transgene S.A. | Pharmaceutical composition for treating papillomavirus tumors and infection |
GB9621091D0 (en) | 1996-10-09 | 1996-11-27 | Fondation Pour Le Perfectionem | Attenuated microorganisms strains and their uses |
FR2766091A1 (en) | 1997-07-18 | 1999-01-22 | Transgene Sa | ANTITUMOR COMPOSITION BASED ON MODIFIED IMMUNOGENIC POLYPEPTIDE WITH CELL LOCATION |
US7494658B2 (en) | 1998-02-20 | 2009-02-24 | Medigene Ag | Papilloma virus truncated L1 protein and fusion protein constructs |
CA2229955C (en) | 1998-02-20 | 2003-12-09 | Medigene Gmbh | Papilloma virus capsomere vaccine formulations and methods of use |
US20020039584A1 (en) | 1998-02-20 | 2002-04-04 | Medigene Ag | Papilloma virus capsomere vaccine formulations and methods of use |
US7182947B2 (en) | 1998-02-20 | 2007-02-27 | Medigene Ag | Papillomavirus truncated L1 protein and fusion protein constructs |
US6926897B1 (en) | 1998-03-24 | 2005-08-09 | Medigene Aktiengesellschaft | Medicament for the avoidance or treatment of papillomavirus-specific tumour |
DE19905883C2 (en) * | 1999-02-11 | 2001-05-23 | Deutsches Krebsforsch | Chimeric virus-like particles or chimeric capsomeres from BPV |
GB9921146D0 (en) * | 1999-09-07 | 1999-11-10 | Smithkline Beecham Biolog | Novel composition |
EE200200709A (en) * | 2000-06-26 | 2004-08-16 | Stressgen Biotechnologies Corporation | Use of a Composition Containing a Fusion Protein and Nucleic Acid Encoding a Fusion Protein and a Fusion Polypeptide |
AU2001275438A1 (en) * | 2000-07-06 | 2002-01-21 | Georgetown University | Stable (fixed) forms of viral capsid proteins, fusion proteins and uses thereof |
GB0206360D0 (en) | 2002-03-18 | 2002-05-01 | Glaxosmithkline Biolog Sa | Viral antigens |
US20080160040A1 (en) * | 2004-04-15 | 2008-07-03 | Ghim Shin-Je | Plant-produced compositions for treating papillomavirus infection and related methods |
TWI457133B (en) | 2005-12-13 | 2014-10-21 | Glaxosmithkline Biolog Sa | Novel composition |
DE102008010954A1 (en) | 2008-02-25 | 2009-08-27 | Cichon, Günter, Prof.Dr. | DNA vaccine for the therapy and prophylaxis of cervical cancer and its premalignant precursors |
CN102099053A (en) | 2008-05-26 | 2011-06-15 | 卡迪拉保健有限公司 | Combined measles-human papilloma vaccine |
EA201592264A1 (en) * | 2009-08-26 | 2016-08-31 | Селекта Байосайенсиз, Инк. | COMPOSITIONS WHICH INDUCE T-CELL HELPER EFFECT |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0133123A1 (en) * | 1983-07-25 | 1985-02-13 | Mgi Pharma, Inc. | Immunogens of papilloma viruses |
US4777239A (en) | 1986-07-10 | 1988-10-11 | The Board Of Trustees Of The Leland Stanford Junior University | Diagnostic peptides of human papilloma virus |
FR2632956B2 (en) | 1988-05-13 | 1991-07-12 | Pasteur Institut | PAPILLOMAVIRUS PROBES HPV49, HPV50, HPV54, HPV55; GENETICALLY AND IMMUNOLOGICALLY RELATED PRODUCTS TO THIS PAPILLOMAVIRUS HPV49, HPV50, HPV54, HPV55; METHOD FOR IN VITRO DIAGNOSIS OF PAPILLOMAVIRUS INFECTIONS AND IN VIVO IMMUNIZATION AGAINST THESE PAPILLOMAVIRUS |
-
1991
- 1991-06-26 GB GB9113809A patent/GB9113809D0/en active Pending
-
1992
- 1992-06-17 DE DE1992629050 patent/DE69229050T2/en not_active Expired - Fee Related
- 1992-06-17 WO PCT/GB1992/001092 patent/WO1993000436A1/en active IP Right Grant
- 1992-06-17 AU AU19859/92A patent/AU662910B2/en not_active Ceased
- 1992-06-17 JP JP50122393A patent/JP3533216B2/en not_active Expired - Fee Related
- 1992-06-17 ES ES92912857T patent/ES2133322T3/en not_active Expired - Lifetime
- 1992-06-17 DK DK92912857T patent/DK0592480T3/en active
- 1992-06-17 AT AT92912857T patent/ATE179458T1/en not_active IP Right Cessation
- 1992-06-17 EP EP92912857A patent/EP0592480B1/en not_active Expired - Lifetime
-
1995
- 1995-06-07 US US08/484,791 patent/US6380157B1/en not_active Expired - Lifetime
-
1998
- 1998-01-10 HK HK98100212A patent/HK1001309A1/en not_active IP Right Cessation
-
1999
- 1999-07-07 GR GR990401799T patent/GR3030716T3/en unknown
-
2002
- 2002-01-18 US US10/054,695 patent/US20020136736A1/en not_active Abandoned
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011100234A2 (en) * | 2010-02-09 | 2011-08-18 | Stc.Unm | Immunogenic hpv l2-containing vlps and related compositions, constructs, and therapeutic methods |
WO2011100234A3 (en) * | 2010-02-09 | 2012-03-15 | Stc.Unm | Immunogenic hpv l2-containing vlps and related compositions, constructs, and therapeutic methods |
US9533057B2 (en) | 2010-02-09 | 2017-01-03 | Stc.Unm | Immunogenic HPV L2-containing VLPs and related compositions, constructs, and therapeutic methods |
US9717783B2 (en) | 2010-02-09 | 2017-08-01 | Stc.Unm | Immunogenic HPV L2-containing VLPs and related compositions, constructs, and therapeutic methods |
WO2013106525A1 (en) * | 2012-01-12 | 2013-07-18 | Stc.Unm | Immunogenic hpv l2-containing vlps and related compositions and methods |
CN104159606A (en) * | 2012-01-12 | 2014-11-19 | Stc.Unm公司 | Immunogenic HPV L2-containing VLPs and related compositions and methods |
Also Published As
Publication number | Publication date |
---|---|
GB9113809D0 (en) | 1991-08-14 |
ATE179458T1 (en) | 1999-05-15 |
JP3533216B2 (en) | 2004-05-31 |
ES2133322T3 (en) | 1999-09-16 |
GR3030716T3 (en) | 1999-11-30 |
US6380157B1 (en) | 2002-04-30 |
DE69229050T2 (en) | 1999-10-21 |
DK0592480T3 (en) | 1999-11-08 |
HK1001309A1 (en) | 1998-06-12 |
AU1985992A (en) | 1993-01-25 |
JPH06508988A (en) | 1994-10-13 |
EP0592480B1 (en) | 1999-04-28 |
WO1993000436A1 (en) | 1993-01-07 |
AU662910B2 (en) | 1995-09-21 |
EP0592480A1 (en) | 1994-04-20 |
DE69229050D1 (en) | 1999-06-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US6380157B1 (en) | Method of treatment using papillomavirus L2 protein | |
Jarrett et al. | Studies on vaccination against papillomaviruses: prophylactic and therapeutic vaccination with recombinant structural proteins | |
EP0796273B1 (en) | Variants of human papilloma virus antigens | |
US5866553A (en) | Polynucleotide vaccine for papillomavirus | |
EP0692028B1 (en) | Pharmaceuticals based on papillomaviruses | |
JP3958360B2 (en) | Polypeptides useful as immunotherapeutic agents and methods of polypeptide preparation | |
US6599508B1 (en) | Papilloma virus-like particles, fusion proteins as well as processes for their production | |
US7754430B2 (en) | Papilloma virus capsomere vaccine formulations and methods of use | |
WO1993020844A1 (en) | Papillomavirus e7 protein | |
CA2305683C (en) | Immunogenic peptides from the hpv e7 protein | |
US6485728B2 (en) | Formalin-Inactivated human papillomavirus L1 protein vaccine | |
JPH06502989A (en) | Herpes simplex virus VP16 vaccine | |
US6174532B1 (en) | L2 immunogenic peptides of papillomavirus | |
US8470372B2 (en) | Material with immunogenicity | |
KR20050050115A (en) | Dna vaccine encoding at least two nonstructural early proteins of papillomavirus | |
AU719837B2 (en) | Pharmaceuticals based on papillomaviruses | |
AU5013099A (en) | Papillomavirus vaccine | |
AU4889102A (en) | Papillomavirus vaccine |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
STCB | Information on status: application discontinuation |
Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION |