US1826467A - Process for the activation of proteases - Google Patents
Process for the activation of proteases Download PDFInfo
- Publication number
- US1826467A US1826467A US376865A US37686529A US1826467A US 1826467 A US1826467 A US 1826467A US 376865 A US376865 A US 376865A US 37686529 A US37686529 A US 37686529A US 1826467 A US1826467 A US 1826467A
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- Prior art keywords
- papain
- proteases
- grams
- activation
- acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
Definitions
- the present invention relates to an improved process for the activation of proteases.
- Vegetable proteases for, example ordinary commercial papain which is obtained from the fruit of Carica papaya,.possess only a small proteolytic activity and generally speaking will not act on peptones and certain albuminoids. sericin cover of raw silk is not attacked. In order to degrade such albuminoids for example by means of papain, it is therefore necessary to add an activator to the papain.
- the only activators of this kind already known are hydrocyanic acid and sulphuretted hydrogen, both of which are unsuitable for many purposes in practice because on account of their poisonous nature troublesome precautions must be taken.
- an activation of vegetable proteases such for example'as papain
- an inorganic compound containing the sulphur thio-acid radicle such as thio- 25 sulphuric acid or salts ofthe same, such as sodium thiosulphate or ammonium thiosulphate, or a tetrathionate which latter is however less active.
- the thio-sulphates have the advantage in contrast with hydrocyanic acid and sulphuretted hydrogen, that they are odorless, non-poisonous and fairly easy to manipulate.
- raw silk for example, is completely degummed in a short time in an acid medium by papain which has been activated by means of thiosulphate.
- the coagulating properties of proteases are increased by additions of thioacids, or'salts thereof, for example sodium thiosulphate.
- thioacids for example sodium thiosulphate.
- the coagulation of rubber latex by means of papain activated with thiosulphate takes place in about a quarterof the time necessary when employing ordinary commercial papain.
- Example 1 cubic centimeters of an E wample 0.1 gram of sodium thiosulphate and 5 cubic centimeters of disodium citrate solution are added to 5 cubic centimeters of fresh pineapple juice (containing the protease bromelin). After 10 minutes a solution of 10 grams of casein in 100 cubic centimeters of water is added and the whole kept at 40 C. 'After 4 hours a solution of a degradation product having an acid value (measured by titration with alcoholic potash) of 350 is obtained.
- Emample 3 100 grams of raw silk are treated-for 1 hour at C. with a solution of 5 grams of papain, '5 grams of sodiumthiosulphate and 2 grams of primary ammonium phosphate in 3 liters of water. The silk is then'removed from the bath and rinsed with a dilute solution of,
- Example 4 10 liters of a practically neutral rubber latexare stirred with a solution of 5 grams of papain, 5 grams of sodium thiosulphate and 4: grams of primary ammonium phosphate in 200 cubic centimeters of water and kept for several hours at a temperature of 30 C. whereby a complete coagulation of the latex is obtained. 4
- an activated protease mixture comprising a vegetable protease and a water-soluble inorganic compound containing a sulphur thio-acid radicle.
- an activated protease mixture comprising a vegetable protease-and a salt of a sulphur thioacid Selected from the group consisting of ammonium and alkali metal salts.
- an activated protease mixture comprising a vege-- table-protease and a thiosulphate belonging to the group consisting of ammonium and alkali metal salts of thiosulphuric acid.
- an activated protease mixture comprising pa aim and a salt of a sulphur thio-acld selected rom the group consisting of ammonium and alkali metal salts.
- an activated protease mixture comprisin pap'ain and a thiosulphate. belonging to t e group 'consisting'of. ammonium and alkali metal salts of thiosulphuric acid.
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Detergent Compositions (AREA)
Description
Patented Dot. 6, 1931 UNITED STATES PATENT I OFFICE AENNE HARTENECK, F MANNHEIM, GERMANY, ASSIGNOR TO I. G. FARBE'NIND'US- TRIE AKTIE-NGESELLSCHAFT, OF FRANKFORT-ON-THE-MAIN, GERMANY, A COR- PORATION 0F GERMANY I PROCESS SEORTHE ACTIVATION OF PROTEASES No Drawing. Application filed July 8, 1929, Serial IIo. 376,865, and in Germany July 14, 1928.
, The present invention relates to an improved process for the activation of proteases.
Vegetable proteases, for, example ordinary commercial papain which is obtained from the fruit of Carica papaya,.possess only a small proteolytic activity and generally speaking will not act on peptones and certain albuminoids. sericin cover of raw silk is not attacked. In order to degrade such albuminoids for example by means of papain, it is therefore necessary to add an activator to the papain. The only activators of this kind already known are hydrocyanic acid and sulphuretted hydrogen, both of which are unsuitable for many purposes in practice because on account of their poisonous nature troublesome precautions must be taken.
I have now found that an activation of vegetable proteases such for example'as papain, is advantageously effected by an addition of an inorganic compound containing the sulphur thio-acid radicle, such as thio- 25 sulphuric acid or salts ofthe same, such as sodium thiosulphate or ammonium thiosulphate, or a tetrathionate which latter is however less active. The thio-sulphates have the advantage in contrast with hydrocyanic acid and sulphuretted hydrogen, that they are odorless, non-poisonous and fairly easy to manipulate. Papain, activated by means of thiosulphates, has a high proteolytic activity, and it is possible to'degrade albuminoids which are unattacked by ordinary commercial papain. Thus raw silk, for example, is completely degummed in a short time in an acid medium by papain which has been activated by means of thiosulphate.
- Moreover the coagulating properties of proteases are increased by additions of thioacids, or'salts thereof, for example sodium thiosulphate. Thus, for example, the coagulation of rubber latex by means of papain activated with thiosulphate takes place in about a quarterof the time necessary when employing ordinary commercial papain.
The following examples will further illustrate how the said invention may be carried Thus for example the,
out in practice, but the invention is not restricted to these examples.
Example 1 cubic centimeters of an E wample 0.1 gram of sodium thiosulphate and 5 cubic centimeters of disodium citrate solution are added to 5 cubic centimeters of fresh pineapple juice (containing the protease bromelin). After 10 minutes a solution of 10 grams of casein in 100 cubic centimeters of water is added and the whole kept at 40 C. 'After 4 hours a solution of a degradation product having an acid value (measured by titration with alcoholic potash) of 350 is obtained.
Emample 3 100 grams of raw silk are treated-for 1 hour at C. with a solution of 5 grams of papain, '5 grams of sodiumthiosulphate and 2 grams of primary ammonium phosphate in 3 liters of water. The silk is then'removed from the bath and rinsed with a dilute solution of,
soap. After the treatment the gum is completely removed.
Example 4 10 liters of a practically neutral rubber latexare stirred with a solution of 5 grams of papain, 5 grams of sodium thiosulphate and 4: grams of primary ammonium phosphate in 200 cubic centimeters of water and kept for several hours at a temperature of 30 C. whereby a complete coagulation of the latex is obtained. 4
4. As new article of manufacture an activated protease mixture comprising a vegetable protease and a water-soluble inorganic compound containing a sulphur thio-acid radicle. i 5. As new article of manufacture an activated protease mixturecomprising a vegetable protease-and a salt of a sulphur thioacid Selected from the group consisting of ammonium and alkali metal salts. 1
6. As new article of manufacture an activated protease mixture comprising a vege-- table-protease and a thiosulphate belonging to the group consisting of ammonium and alkali metal salts of thiosulphuric acid.
7 As new article of manufacture an activated protease mixture comprising papain and a water-soluble inorganic compound containing a sulphur thio-acid radicle.
8. As new article of manufacture an activated protease mixture comprising pa aim and a salt of a sulphur thio-acld selected rom the group consisting of ammonium and alkali metal salts. a
9. As new article of manufacture an activated protease mixture comprisin pap'ain and a thiosulphate. belonging to t e group 'consisting'of. ammonium and alkali metal salts of thiosulphuric acid.
10. As new article of manufacture an activated protease mixture comprising papain and sodium thiosulphate. p
In testimony *whereof I have hereunto set my hand.
AENNE HARTENECK.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE1826467X | 1928-07-14 |
Publications (1)
Publication Number | Publication Date |
---|---|
US1826467A true US1826467A (en) | 1931-10-06 |
Family
ID=7744962
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US376865A Expired - Lifetime US1826467A (en) | 1928-07-14 | 1929-07-08 | Process for the activation of proteases |
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US (1) | US1826467A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2457754A (en) * | 1943-04-22 | 1948-12-28 | Irwin W Tucker | Process of extracting diastase |
US2958632A (en) * | 1955-12-28 | 1960-11-01 | Schwarz Lab Inc | Process for recovery of highly active, stable, substantially sterile, soluble, proteolytic, enzyme composition from commercial crude papain |
US3220928A (en) * | 1962-07-06 | 1965-11-30 | Schwarz Lab Inc | Enzymatic cleaning process |
US6066481A (en) * | 1996-03-08 | 2000-05-23 | Novo Nordisk A/S | Crystallization of a protein with a sulphur salt |
-
1929
- 1929-07-08 US US376865A patent/US1826467A/en not_active Expired - Lifetime
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2457754A (en) * | 1943-04-22 | 1948-12-28 | Irwin W Tucker | Process of extracting diastase |
US2958632A (en) * | 1955-12-28 | 1960-11-01 | Schwarz Lab Inc | Process for recovery of highly active, stable, substantially sterile, soluble, proteolytic, enzyme composition from commercial crude papain |
US3220928A (en) * | 1962-07-06 | 1965-11-30 | Schwarz Lab Inc | Enzymatic cleaning process |
US6066481A (en) * | 1996-03-08 | 2000-05-23 | Novo Nordisk A/S | Crystallization of a protein with a sulphur salt |
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