UA90939U - Method for determination of catalytic efficiency of transglutaminase preparation - Google Patents
Method for determination of catalytic efficiency of transglutaminase preparationInfo
- Publication number
- UA90939U UA90939U UAU201401090U UAU201401090U UA90939U UA 90939 U UA90939 U UA 90939U UA U201401090 U UAU201401090 U UA U201401090U UA U201401090 U UAU201401090 U UA U201401090U UA 90939 U UA90939 U UA 90939U
- Authority
- UA
- Ukraine
- Prior art keywords
- solution
- transglutaminase
- mixture
- determination
- hours
- Prior art date
Links
- 108060008539 Transglutaminase Proteins 0.000 title abstract 5
- 102000003601 transglutaminase Human genes 0.000 title abstract 5
- 230000003197 catalytic effect Effects 0.000 title abstract 3
- 238000002360 preparation method Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 3
- 239000000203 mixture Substances 0.000 abstract 3
- 238000000855 fermentation Methods 0.000 abstract 2
- 230000004151 fermentation Effects 0.000 abstract 2
- 239000000499 gel Substances 0.000 abstract 2
- 108010016626 Dipeptides Proteins 0.000 abstract 1
- 108010010803 Gelatin Proteins 0.000 abstract 1
- 108010024636 Glutathione Proteins 0.000 abstract 1
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 abstract 1
- 238000010586 diagram Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 238000011156 evaluation Methods 0.000 abstract 1
- 235000013305 food Nutrition 0.000 abstract 1
- 239000008273 gelatin Substances 0.000 abstract 1
- 229920000159 gelatin Polymers 0.000 abstract 1
- 235000019322 gelatine Nutrition 0.000 abstract 1
- 235000011852 gelatine desserts Nutrition 0.000 abstract 1
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
- 230000035515 penetration Effects 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The method for determination of catalytic efficiency of transglutaminase preparation provides that to the solution of dipeptide N-CZ-GLN-GLY in the mixture having pH in interval 6.00-6.10 containing NaOH, hydroxylamine and reduced glutathione in the ratio of 1:2:2 with total volume of 15 ml, 0.1 ml of transglutaminase sample is added. The mixture is thermostated at 37° C for 10 minutes, cooled and 15 ml of mixture of 3% solution is added into 0.1 N HCl, 12 % solution of trichloroacetic acid HCl and in the ratio in equal amount, is centrifuged. The value of optical density is determined in liquid part at 525 nm, using for comparison analogous solution without transglutaminase, activity values are calculated relative to calibrating diagram, built with using of chemically clean transglutaminase. The catalytic activity is determined according to the rate of penetration of gel of standardized 10% solution of food gelatin with pH 7.4. Evaluation in wide range of values mass fraction of enzymatic preparation in enzyme-substrate system is 0.5%, fermentation time at the temperature of 50 °C – 24 hours, and gel fermentation time at the temperature of 25 °C – 12 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAU201401090U UA90939U (en) | 2014-02-05 | 2014-02-05 | Method for determination of catalytic efficiency of transglutaminase preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAU201401090U UA90939U (en) | 2014-02-05 | 2014-02-05 | Method for determination of catalytic efficiency of transglutaminase preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA90939U true UA90939U (en) | 2014-06-10 |
Family
ID=89901582
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UAU201401090U UA90939U (en) | 2014-02-05 | 2014-02-05 | Method for determination of catalytic efficiency of transglutaminase preparation |
Country Status (1)
| Country | Link |
|---|---|
| UA (1) | UA90939U (en) |
-
2014
- 2014-02-05 UA UAU201401090U patent/UA90939U/en unknown
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