UA81564C2 - Method for differentiation of human stromal stem cells in cultural medium in vitro - Google Patents

Method for differentiation of human stromal stem cells in cultural medium in vitro

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Publication number
UA81564C2
UA81564C2 UAA200607514A UAA200607514A UA81564C2 UA 81564 C2 UA81564 C2 UA 81564C2 UA A200607514 A UAA200607514 A UA A200607514A UA A200607514 A UAA200607514 A UA A200607514A UA 81564 C2 UA81564 C2 UA 81564C2
Authority
UA
Ukraine
Prior art keywords
cells
differentiation
cultural
cultural medium
stem stromal
Prior art date
Application number
UAA200607514A
Other languages
Russian (ru)
Ukrainian (uk)
Inventor
Вера Семеновна Астахова
Original Assignee
Вера Семеновна Астахова
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Вера Семеновна Астахова filed Critical Вера Семеновна Астахова
Priority to UAA200607514A priority Critical patent/UA81564C2/en
Publication of UA81564C2 publication Critical patent/UA81564C2/en

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Abstract

The invention relates to biotechnology and medicine, in particular to orthopedy and traumatology, and may be used with the aim of the differentiation of human stem stromal cells. A method for differentiation of stem stromal cells in cultural medium provides for the preparation of a suspension of marrow cells from removed human bones, determination of concentration of nuclea-containing cells under conventional technique, addition of obtained suspension into cultural medium for cultivation of stem stromal cells, wherein, according to the invention, for differentiation of human stem stromal cells prior to suspense preparation cells of rabbit marrow cells are radiated with lethal action and added one time into nutrient medium in the absence of growth factors and further change of cultural medium in quantity of not les than 5х10nucleacontaining cells per 1 cmof «cultural» vessel bottom area, at that nucleacontaining cells are washed out mechanically without use of enzymes, material is fixed for certain period depending on culture maturity and dinamycs of stem stromal cell differentiation is determined. The use of rabbit marrow frozen at the temperature of not more than -20 °С and activated by defrosting at room temperature in the absence of activation agent, promotes the increase of productivity of method and culture.
UAA200607514A 2006-07-05 2006-07-05 Method for differentiation of human stromal stem cells in cultural medium in vitro UA81564C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
UAA200607514A UA81564C2 (en) 2006-07-05 2006-07-05 Method for differentiation of human stromal stem cells in cultural medium in vitro

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
UAA200607514A UA81564C2 (en) 2006-07-05 2006-07-05 Method for differentiation of human stromal stem cells in cultural medium in vitro

Publications (1)

Publication Number Publication Date
UA81564C2 true UA81564C2 (en) 2008-01-10

Family

ID=39311906

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA200607514A UA81564C2 (en) 2006-07-05 2006-07-05 Method for differentiation of human stromal stem cells in cultural medium in vitro

Country Status (1)

Country Link
UA (1) UA81564C2 (en)

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