UA81564C2 - Method for differentiation of human stromal stem cells in cultural medium in vitro - Google Patents
Method for differentiation of human stromal stem cells in cultural medium in vitroInfo
- Publication number
- UA81564C2 UA81564C2 UAA200607514A UAA200607514A UA81564C2 UA 81564 C2 UA81564 C2 UA 81564C2 UA A200607514 A UAA200607514 A UA A200607514A UA A200607514 A UAA200607514 A UA A200607514A UA 81564 C2 UA81564 C2 UA 81564C2
- Authority
- UA
- Ukraine
- Prior art keywords
- cells
- differentiation
- cultural
- cultural medium
- stem stromal
- Prior art date
Links
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to biotechnology and medicine, in particular to orthopedy and traumatology, and may be used with the aim of the differentiation of human stem stromal cells. A method for differentiation of stem stromal cells in cultural medium provides for the preparation of a suspension of marrow cells from removed human bones, determination of concentration of nuclea-containing cells under conventional technique, addition of obtained suspension into cultural medium for cultivation of stem stromal cells, wherein, according to the invention, for differentiation of human stem stromal cells prior to suspense preparation cells of rabbit marrow cells are radiated with lethal action and added one time into nutrient medium in the absence of growth factors and further change of cultural medium in quantity of not les than 5х10nucleacontaining cells per 1 cmof «cultural» vessel bottom area, at that nucleacontaining cells are washed out mechanically without use of enzymes, material is fixed for certain period depending on culture maturity and dinamycs of stem stromal cell differentiation is determined. The use of rabbit marrow frozen at the temperature of not more than -20 °С and activated by defrosting at room temperature in the absence of activation agent, promotes the increase of productivity of method and culture.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
UAA200607514A UA81564C2 (en) | 2006-07-05 | 2006-07-05 | Method for differentiation of human stromal stem cells in cultural medium in vitro |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
UAA200607514A UA81564C2 (en) | 2006-07-05 | 2006-07-05 | Method for differentiation of human stromal stem cells in cultural medium in vitro |
Publications (1)
Publication Number | Publication Date |
---|---|
UA81564C2 true UA81564C2 (en) | 2008-01-10 |
Family
ID=39311906
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
UAA200607514A UA81564C2 (en) | 2006-07-05 | 2006-07-05 | Method for differentiation of human stromal stem cells in cultural medium in vitro |
Country Status (1)
Country | Link |
---|---|
UA (1) | UA81564C2 (en) |
-
2006
- 2006-07-05 UA UAA200607514A patent/UA81564C2/en unknown
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