UA69925A - Method for assaying activity of trypsin-like proteolytic enzymes in human blood serum - Google Patents
Method for assaying activity of trypsin-like proteolytic enzymes in human blood serumInfo
- Publication number
- UA69925A UA69925A UA20031211665A UA20031211665A UA69925A UA 69925 A UA69925 A UA 69925A UA 20031211665 A UA20031211665 A UA 20031211665A UA 20031211665 A UA20031211665 A UA 20031211665A UA 69925 A UA69925 A UA 69925A
- Authority
- UA
- Ukraine
- Prior art keywords
- blood serum
- trypsin
- human blood
- proteolytic enzymes
- activity
- Prior art date
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The method for assaying the activity of trypsin-like proteolytic enzymes in human blood serum provides for the enzymatic hydrolysis of the substrate with proteinases producing para-nitroaniline. Then the product content is measured spectrophotometrically. N-a-benzoil-DL-aginine-para-nitroanilide hydrochloride is used as a substrate. The enzymatic assay is performed in the wells of the polystyrene plates or strips intended for immunoenzyme assays. The optical density of the control and assayed samples is measured employing the digital EIA reader and the proteolytic activity in blood serum is finally calculated.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
UA20031211665A UA69925A (en) | 2003-12-16 | 2003-12-16 | Method for assaying activity of trypsin-like proteolytic enzymes in human blood serum |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
UA20031211665A UA69925A (en) | 2003-12-16 | 2003-12-16 | Method for assaying activity of trypsin-like proteolytic enzymes in human blood serum |
Publications (1)
Publication Number | Publication Date |
---|---|
UA69925A true UA69925A (en) | 2004-09-15 |
Family
ID=34512916
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
UA20031211665A UA69925A (en) | 2003-12-16 | 2003-12-16 | Method for assaying activity of trypsin-like proteolytic enzymes in human blood serum |
Country Status (1)
Country | Link |
---|---|
UA (1) | UA69925A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2623875C1 (en) * | 2016-02-19 | 2017-06-29 | Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский и технологический институт птицеводства" | Method for biochemical determination of proteolytic enzymes activity in blood |
-
2003
- 2003-12-16 UA UA20031211665A patent/UA69925A/en unknown
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2623875C1 (en) * | 2016-02-19 | 2017-06-29 | Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский и технологический институт птицеводства" | Method for biochemical determination of proteolytic enzymes activity in blood |
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