UA69925A - Method for assaying activity of trypsin-like proteolytic enzymes in human blood serum - Google Patents

Method for assaying activity of trypsin-like proteolytic enzymes in human blood serum

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Publication number
UA69925A
UA69925A UA20031211665A UA20031211665A UA69925A UA 69925 A UA69925 A UA 69925A UA 20031211665 A UA20031211665 A UA 20031211665A UA 20031211665 A UA20031211665 A UA 20031211665A UA 69925 A UA69925 A UA 69925A
Authority
UA
Ukraine
Prior art keywords
blood serum
trypsin
human blood
proteolytic enzymes
activity
Prior art date
Application number
UA20031211665A
Inventor
Anatolii Mykolaiovy Bondarenko
Original Assignee
Anatolii Mykolaiovy Bondarenko
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anatolii Mykolaiovy Bondarenko filed Critical Anatolii Mykolaiovy Bondarenko
Priority to UA20031211665A priority Critical patent/UA69925A/en
Publication of UA69925A publication Critical patent/UA69925A/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The method for assaying the activity of trypsin-like proteolytic enzymes in human blood serum provides for the enzymatic hydrolysis of the substrate with proteinases producing para-nitroaniline. Then the product content is measured spectrophotometrically. N-a-benzoil-DL-aginine-para-nitroanilide hydrochloride is used as a substrate. The enzymatic assay is performed in the wells of the polystyrene plates or strips intended for immunoenzyme assays. The optical density of the control and assayed samples is measured employing the digital EIA reader and the proteolytic activity in blood serum is finally calculated.
UA20031211665A 2003-12-16 2003-12-16 Method for assaying activity of trypsin-like proteolytic enzymes in human blood serum UA69925A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
UA20031211665A UA69925A (en) 2003-12-16 2003-12-16 Method for assaying activity of trypsin-like proteolytic enzymes in human blood serum

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
UA20031211665A UA69925A (en) 2003-12-16 2003-12-16 Method for assaying activity of trypsin-like proteolytic enzymes in human blood serum

Publications (1)

Publication Number Publication Date
UA69925A true UA69925A (en) 2004-09-15

Family

ID=34512916

Family Applications (1)

Application Number Title Priority Date Filing Date
UA20031211665A UA69925A (en) 2003-12-16 2003-12-16 Method for assaying activity of trypsin-like proteolytic enzymes in human blood serum

Country Status (1)

Country Link
UA (1) UA69925A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2623875C1 (en) * 2016-02-19 2017-06-29 Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский и технологический институт птицеводства" Method for biochemical determination of proteolytic enzymes activity in blood

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2623875C1 (en) * 2016-02-19 2017-06-29 Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский и технологический институт птицеводства" Method for biochemical determination of proteolytic enzymes activity in blood

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