SE0302840D0 - Detection method - Google Patents
Detection methodInfo
- Publication number
- SE0302840D0 SE0302840D0 SE0302840A SE0302840A SE0302840D0 SE 0302840 D0 SE0302840 D0 SE 0302840D0 SE 0302840 A SE0302840 A SE 0302840A SE 0302840 A SE0302840 A SE 0302840A SE 0302840 D0 SE0302840 D0 SE 0302840D0
- Authority
- SE
- Sweden
- Prior art keywords
- substrate
- cell
- beta
- heterologous
- prokaryotic cell
- Prior art date
Links
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A method for determining whether , beta-lactamase activity is present in a prokaryotic cell is provided, which comprises the steps of: providing a fluorescent substrate for , beta-lactamase; providing a prokaryotic cell which comprises a gene encoding a heterologous esterase enzyme, which enzyme has the ability to convert the substrate from a diffusing form to an active form; applying said substrate, in its diffusing form, to said prokaryotic cell, said application enabling said heterologous esterase enzyme to convert the substrate to its active form; culturing said cell under conditions such that any beta-lactamase activity present in the cell cleaves said substrate, yielding a fluorescent signal; and detecting fluorescence from said cell.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE0302840A SE0302840D0 (en) | 2003-10-29 | 2003-10-29 | Detection method |
PCT/SE2004/001563 WO2005040412A1 (en) | 2003-10-29 | 2004-10-28 | Detection method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE0302840A SE0302840D0 (en) | 2003-10-29 | 2003-10-29 | Detection method |
Publications (1)
Publication Number | Publication Date |
---|---|
SE0302840D0 true SE0302840D0 (en) | 2003-10-29 |
Family
ID=29580143
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SE0302840A SE0302840D0 (en) | 2003-10-29 | 2003-10-29 | Detection method |
Country Status (2)
Country | Link |
---|---|
SE (1) | SE0302840D0 (en) |
WO (1) | WO2005040412A1 (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8097434B2 (en) | 2007-10-19 | 2012-01-17 | Becton, Dickinson And Company | Methods for the detection of beta-lactamases |
US20110020240A1 (en) * | 2008-08-06 | 2011-01-27 | Cirillo Jeffrey D | Use of bacterial beta-lactamase for in vitro diagnostics and in vivo imaging, diagnostics and therapeutics |
US9834807B2 (en) | 2008-10-20 | 2017-12-05 | Becton, Dickinson And Company | Compositions for the detection of intracellular bacterial targets and other intracellular micororganism targets |
CN104714006B (en) * | 2015-02-04 | 2017-10-20 | 国家纳米科学中心 | A kind of method of beta lactamase in detection dairy produce |
US11718868B2 (en) * | 2020-01-31 | 2023-08-08 | The Government Of The United States Of America, As Represented By The Secretary Of The Navy | Nanoparticle-based lipase biosensor utilizing a custom-synthesized peptidyl-ester substrate |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5338843A (en) * | 1992-01-30 | 1994-08-16 | Becton, Dickinson And Company | Fluorogenic and chromogenic β-lactamase substrates |
US5928888A (en) * | 1996-09-26 | 1999-07-27 | Aurora Biosciences Corporation | Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities |
JP2003523204A (en) * | 2000-02-17 | 2003-08-05 | メルク エンド カムパニー インコーポレーテッド | Cell fusion assay using fluorescence resonance energy transfer |
-
2003
- 2003-10-29 SE SE0302840A patent/SE0302840D0/en unknown
-
2004
- 2004-10-28 WO PCT/SE2004/001563 patent/WO2005040412A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2005040412A1 (en) | 2005-05-06 |
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