UA66570U - method for THE preparation of pharmaceutical composition of wound healing and regenerating action based on peptidE bioregulators - Google Patents

Abstract

A method for the preparation of pharmaceutical composition of wound healing and regenerating action based on peptide bioregulators, wherein the low-molecular peptides of dermal layer of pig skin are incorporated in liposomes. At first low-molecular peptides are prepared by freezing of dermal layer of pig skin, its grinding, extraction, ultra filtration and concentration, then produced liposomes with the particle size of 90-120 and 100-120 nm are prepared by dissolution of lecithin in alcohol, evaporation in rotary film evaporator, washing off with water at alcohol content no more than 8 %, pH is brought to 4.0-4.2. A mixture of low-molecular peptides produced at the stage of ultra filtration is added to the liposome suspension, the peptides are included to liposomes and transferred to the stage of sterilizing filtration by a method of constant flow on a membrane with pore diameter of 0.2 µm, and after that they are bottled.

Description

Local therapy depends on the stage at which liposomes are injected with a mixture of low molecular weight ulcers or wounds. For the treatment of viral peptides obtained at the stage of ultrafiltration, wounds or wounds that do not heal well, their inclusion in liposomes is used, and they are transferred to drugs that are tissue-specific stimulants, the stage of sterilizing filtration, filter regeneration measures and affect regeneration and so on constant flow on the membrane with a diameter of skin repair. In most cases, the pores are 0.2 μm. Bottling, obtaining a spray. The drug, which has the ability to stimulate skin regeneration, is poured into bottles of 25 ml. is combined with other effects: anti-inflammatory Example of a specific implementation action (comfrey and calendula ointment), antioxidant - Obtaining low-molecular peptides. (Revalid), by restoring the balance of coagulation The dermal layer of pig skin, pre-blood, improving local microcirculation and frozen, is crushed on a cryo-shredder of skin trophs (Hepatrombin) and antibacterial for 20 seconds, speed 3500 rpm. (Polyvinox, zinc hyaluronate, Phytostimulin, etc.) Extraction.

The main purpose of prescribing these drugs is to weigh the crushed skin, load it into a thermal and functional capacity, add cooled water (4-6 C) to the tissue in the affected area. in a ratio of 1:10 and adjust the pH with acid so-

However, it should be noted that it is fast and effective concentrated to 5.2-5.4. Put a poultice in the healing of skin with epithelization and stir for 2 hours through a liquid in the presence of surface damage (erosion every 30 minutes. The extract is left for 12 hours in sion, ulcers, burns, etc.). Deeper lesions of the refrigerator at a temperature of 4-6 "C. After the skin heals with the formation of connective tissue, the supernatant is decanted, filtered and transferred to ultrafiltration.

Neurometabo- Ultrafiltration also has a regenerative effect. personal stimulants, such as Cerebrolysin, Vinpoce- Ultrafiltration is carried out on a membrane of 5 kD tin. The drug Actovegin - purified from protein ext- at a temperature of 6-8 70. ract from the blood of calves, contains low molecular weight Permeate is transferred to the concentration stage. peptides and derivatives of nucleic acids. Apply- Concentration. Actovegin is used for ulcers of various origins. The concentration is carried out on rotator bedsores, burns, corneal and scleral injuries, and a film vaporizer. also for the healing of infected wounds, with radiation - Concentrate 60 times. skin lesions, during skin transplantation. Preparation of liposomes with a particle size of 90-

The use of substances containing biologically 120 nm active molecules obtained from humans or other Mammalian production of liposomes with a particle size of 100 already has the prospect of clinical 120 nm application. Dissolve lecithin in alcohol, evaporate on

On the day of writing the application, the authors did not detect any sources where a similar lipid film was described before the formation of the rotor-film evaporator, then the lipid film is washed off with a pharmaceutical preparation for injections. The alcohol content should not be a rat with a similar activity, so the reference is more than 8 95, adjust the pH to 5.0-5.22, obtain the drug selected "Solcoseryl" the closest to liposomes with a particle size of 90-120 nm. known for its regenerating and wound-healing activity. Preparation of the drug. tyu, but completely different in composition and technology. Into the liposome suspension, a mixture is introduced with a low production technology. of molecular peptides obtained at the UV stage.

Technical task - implementation in the medical stage of sterilizing filtration. means with regenerating and wound-healing mechanism - Sterilizing filtration. duration of action and high bioavailability. Filter by the method of constant flow on

The task is solved by a newly formed membrane with a pore diameter of 0.2 μm. It is poured into bottles of 25 ml by the method of obtaining a pharmaceutical composition. zation of wound-healing and regenerating action based on the Technical result - a group of authors created peptide bioregulators, which consists in the fact that a new method of obtaining a drug from re- low-molecular peptides of the dermal layer with a generating and wound-healing mechanism of action and skin of pigs is incorporated into liposomes, and with high bioavailability. The conducted studies initially obtain low-molecular-weight peptides on animals, confirm high efficiency by freezing the dermal layer of a pig - obtained according to a useful model of the drug, in which skin, its grinding, extraction, ultrafiltration - compared with the reference drug "Solcoserations and concentrations, then receive liposomes from the gills ". with a particle size of 90-120 and 100-120 nm by the method In table. 1 presents data on the study of the effect of dissolving lecithin in alcohol, evaporation on the drug under study using a useful model of a rotary-film evaporator, washing off the film and the reference drug "Solkoseryl" on mice with water (the alcohol content should be no more than signs of endogenous intoxication in animals with - 8 be), bring the pH up to 5.0-5.2, by obtaining liposomes caused by skin damage. with a particle size of 90-120 nm, after which in the

Table

Indicators of endogenous intoxication in the studied groups of experimental animals at different periods of observation (M z t) 111111 Grulytvaryn.// | Days | EP | 77777 Kim

Pntayetnia 77777711 1111710111117111111158,2540y5 | 0823003

Skin lesions without correction

Skin lesions from the drug according to the same model

Skin lesions same as "Solcoseryl"

Note. Values that are statistically significantly different from similar values in a group of practically healthy animals are marked with an asterisk. Grids indicate values that are statistically significantly different from similar values in the group of untreated animals with skin damage. yes - re 0.001

YAN - re 0.001

The highest degree of erythrocyte damage with predominant accumulation of markers of destructive membranes in rats with simulated post-damaging processes over restorative-reparative ones. Skin irritation was observed on the 3rd day from the moment. The conducted studies showed that the damage of modeling the wound and increased by 51.3 95. Skin irritation of animals leads to an increase compared to intact rats. In animals from the SMP ura fraction with a higher molecular weight, which, from the first day of the skin, received pre- products of protein-enzyme degradation, the nucleopair according to a useful model, the EII statistically of natides and structural proteins. In rats with simulated skin damage, the content of SMP in the serum of intact animals exceeded the similar indicator in the group, but it was 16.2 95 lower than in blood undergoing significant changes. On the third day of the experiment in animals with an untreated wound. A similar dynamic - this leads to an increase in Ksmp from (0.8250.03) to ka was also observed when using the reference (1.94:30.10), which is 136.6 95 higher than the similar drug "Solcoseryl". In the group of animals with disease, the parameter in rats of the intact group. On the 7th and 14th days of the skin, which as a corrective factor received - observation in the group of untreated animals, Kemp took this drug, EY! was 19.9 95 lower than in the group of untreated animals. healthy animals by 107.3 95 and 75.6 905 rest

On the following days of the experiment (7 and 14 days), respectively. positive changes during the use of the studied drug and the use of the studied drug compared to the reference drug were even more expressive- a useful model contributed to the reduction of Ksmp in all of them and showed a steady tendency to decrease the EIIY during the observation period and to bring it closer to the po- in relation to the similar indicator in the group of the executor of intact animals groups animals without treatment of skin lesions. According to the absolute Thus, when using the tested amount on the 14th day of the EP study, we noted its positive effect in both groups of experimental animals, which led to a decrease in the level of toxemia at the expense of the norm. decrease in the permeability of erythrocyte membranes

The SMP coefficient (where SMP is medium-molecular and a decrease in the content of medium-molecular peplar peptides) is an important prognostic criterion. swarm, which allows to evaluate the course of pathological Industrial application. The group of authors of the process and activity of reparative-restorative func- prepared several experimental samples of the drug for the body. In practically healthy individuals, this is a useful model on the basis of OJSC "PHARMAK", so the indicator is close to 1.0 (0.8-1.0); its increase in the level of specific activity carried out at the base indicates an unfavorable course of the disease of the Ternopil Medical University. Industrial implementation is being prepared.

Computer layout L. Lytvynenko Signed Circulation 23 approx.

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