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Measuring Or Testing Involving Enzymes Or Micro-Organisms
(AREA)
Abstract
A method for determining the varietal purity and uniformity of seed lots of agricultural plants using DNA-markers includes creating test-system by means of selecting polymorphous DNA-markers, isolating DNA of a tested plant specimen, carrying out amplification of DNA-markers in polymerase chain reaction, testing the amplification products. DNA is isolated simultaneously from a representative totality of the seed of the variety being assessed. Testing the amplification products is carried out by means of determining qualitative and quantitative composition of alleles of marker sequences, and the obtained ratios of the alleles are used for calculating the varietal purity (uniformity) of a seed lot.
UAU200711049U2007-10-082007-10-08Method for determining varietal purity and typicalness of lots of seed of farm crops using dna-markers
UA56555U
(en)
Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Елецкий государственный университет им. И.А. Бунина"
Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Елецкий государственный университет им. И.А. Бунина"
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