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Application filed by Олексій Павлович Лазарєв, Алексей Павлович Лазарев, Віктор Семьонович Карасьов, Олександр Вікторович Стефанов, Александр Викторович Стефанов, Віталій Іванович Крюков, Микола Якович Співак, Николай Яковлевич Спивак, Сергій Олександрович Возіанов, Сергей Александрович Возианов, Євгенія Адольфівна Олейнікова, Віктор Олександрович Рибчук, Виктор Александрович РибчукfiledCriticalОлексій Павлович Лазарєв
Priority to UA99020636ApriorityCriticalpatent/UA33795A/en
Publication of UA33795ApublicationCriticalpatent/UA33795A/en
Medicines That Contain Protein Lipid Enzymes And Other Medicines
(AREA)
Abstract
The invention relates to chemical-pharmaceutical (medical) industry. A process for preparation of insulin consists in the following. The crushed pancreas is extracted with 60-85 % alcohol solution, centrifuged, ballast proteins are settled, filtered and obtained solution is entered into a column with sulphocationite. The washing up of ballast proteins is carried out by ammonium and acetate buffer with pH 6.0, and the elution is carried out with 0.1-1.0 M carbonate buffer with pH 6.5-9.0. The separation of proinsulin and other polymeric proteins is carried out on a sorbent based on silicagel with graft hexadecyl groups, and as eluent aqueous solutions containing isopropyl alcohol (10-50 %) and acetic acid (0.1-5.0 %) are used. The purification from desamidated and other forms of insulin is carried out in the gradient of aqueous-organic eluent with the content of acetic acid from 0.2 up to 0.8 %.
UA99020636A1999-02-041999-02-04
METHOD OF OBTAINING INSULIN
UA33795A
(en)