UA23265U - Culture medium for bacteria growth in the air of industrial premises - Google Patents

Abstract

A culture medium for growth of bacteria which are in the air of industrial premises contains dry fermentative peptone, agar-agar, water and additionally contains dry adapted milk formula with iron -Nestogen.

Description

Description of the invention

The useful model relates to medical and veterinary microbiology and can be used in 2 research and production laboratories of a medical and veterinary profile to determine the number of microorganisms in the air of industrial premises.

In medical and veterinary microbiology, there are known methods of estimating the number of microorganisms in the air of industrial premises and nutrient media for their isolation.

The closest to the declared nutrient medium is the nutrient medium for the growth of bacteria found in the air of production facilities - meat peptone agar (MPA), which contains dry fermentative peptone, agar-agar and water | see Asonov N.R. Workshop on microbiology. M. Agropromizdat, 1998. P.72-75).

The disadvantage of the nutrient medium is the slow growth of bacteria on it, which prolongs the period of exposure of the seed material in the thermostat.

The useful model is based on the task of creating such a nutrient environment for the growth of bacteria, which 72 are in the air of industrial premises, in which, due to the new composition of components and their quantitative ratio, it became possible to reduce the duration of incubation of the material to 24 hours, and to reduce the time of conducting research by 50905 .

To solve the problem, a nutrient medium for the growth of bacteria found in the air of industrial premises is proposed, which contains dry enzymatic peptone, agar-agar, water, which, according to the useful model 20, additionally contains a dry adapted milk mixture with iron - Meviodep at this ratio , mass. 95; agar-agar 1-2; dry enzymatic peptone 3-6; Meviodep 0.5-1.0; the rest is water.

In the preferred version, the iron content in 1000 ml of the finished nutrient medium is at least 0.06-0.18 mg.

Due to the new features: the new composition of the nutrient medium, a synergistic effect is created, which causes a reduction in the duration of incubation of the material with a simultaneous increase in the sensitivity of the method, and as a result - a significant reduction in the duration of research by 5095. The effectiveness of the proposed nutrient medium lies in its specificity, accelerated growth in primary crops of material. As a result of research, it was established that on the environment used as a prototype, the growth of bacteria from the air appeared after 24 hours, after 48 hours. their number is increasing significantly. The growth of all cultures was noted on the proposed medium 24 hours after 30 o'clock thermostating. After 72 hours, lawn growth of colonies was observed. Gee)

Specific examples.

Example 1 p

Experiments were carried out at minimum concentrations of all components of the nutrient medium, wt.bo: so agar-agar 1.0; dry enzymatic peptone 3.0; Mesiodepe 0.5; the rest is water.

In parallel, meat peptone agar was prepared according to the generally accepted method (prototype). Determination of bacteria in the air in the room was carried out according to Koch's method. Prepared Petri dishes with control (prototype) and experimental medium were placed in different places of the room together with control and experimental medium and opened for 5 minutes, and then closed. Experimental cups with crops were treated with electromagnetic irradiation, with a Hertz power of 50 W. for 30 minutes, after which it was thermostated for 24-48 hours at a temperature of 36-4C. The results were calculated after 24 and 48 hours. c The microbial number of the air was determined after counting the colonies that had grown using Omelyanskyi's "c" rule. Count the number of colonies that grew on the media and make calculations per tm From the air. According to Omelyansky, on an area of 100 cm 2 within 5 minutes. the amount of microorganisms that is contained in 1Ol settles. air.

As a result of the assessment (after 24 hours of incubation in a thermostat) in the summer period, the number of microorganisms in the air in a closed room was in thousands/m? with the use of the prototype (MPA) - 2.45 - 0.31 m.t., while on the proposed environment this indicator was - 3.89 - 0.20 m.t. The same pattern was observed after 48 hours. incubation in the thermostat of the control and experimental medium at 50 °C, that is, the control was 3.17-0.2 bm.t., and the experimental indicator almost did not change - 3.91--0.24 m.t., which indicates stimulating properties of the proposed method. sl It should be determined that at the end of the third day, lawn growth appeared on the experimental medium, and the diameter of the colony increased on the prototype medium.

Example 2

The research methodology was carried out according to the scheme, as in example 1, but the number of components was average within the indicated intervals. So, for the nutrient medium, this amount was, mass.9o: agar-agar 1.5; dry enzymatic peptone 4.0; Meviodep 0.5; the rest is water. The effect of electromagnetic radiation was for 40 minutes. at 8 hertz and 50 W. The results of the research in comparison with example 1 had no significant difference.

Example C 6o0 The research methodology was carried out according to the scheme as in example 1, but the number of components in the nutrient medium was maximal within the specified intervals. So, for the nutrient medium, this amount was, wt.9o: agar-agar 2.0; dry enzymatic peptone 6.0; Mesiodepe 1.0; the rest is water.

The test cups were treated with electromagnetic radiation with Hertz power of 5O0W for 60 minutes, after which they were thermostated. 65 As a result of the research, it was established that the growth of colonies After 24 hours was more intensive than in example 1 on experimental cups, but no reliably significant difference was observed in terms of the number of colonies obtained. Lawn growth appeared on the second day on experimental Petri dishes.

The proposed nutrient medium is simple in its preparation, shortens the research period, and is convenient for storage and transportation. All components of the declared components are sold in Ukraine, which is enough

Convenient for production, scientific research laboratories of veterinary and medical profile.

Claims (2)

Formula of the invention 70 1. Nutrient medium for the growth of bacteria found in the air of industrial premises, containing dry fermentative peptone, agar-agar, water, which is distinguished by the fact that it additionally contains a dry adapted milk mixture with iron - Meviodep at this ratio, mass for: agar-agar 1-2, dry fermentative peptone 3-6, Meviodep 0.5-1.0, water - the rest. 2. Nutrient medium according to claim 1, which differs in that it has an iron content of at least 0.06-0.18 mg in 100 ml of the finished nutrient medium. Official bulletin "Industrial Property". Book 1 "Inventions, useful models, topographies of integrated microcircuits", 2007, M 6, 10.05.2007. State Department of Intellectual Property of the Ministry of Education and Science of Ukraine. - IS) (Se) s (ze) s - with . and? name) (95) name) Fo sl p 60 b5