UA145389U - METHOD OF GIVING FIBER MATERIALS ANTIBACTERIAL PROPERTIES - Google Patents

Abstract

A method of imparting antibacterial properties to fibrous materials using an aqueous solution of a biocidal N, S-containing organic compound. The process is carried out by impregnating the fibrous material, and as a biocidal N, S-containing organic compound using one of the aminomethanesulfonic acids of General formula RNHCH2SO3H, where R is H, alkyl.

Description

The utility model belongs to the field of production of fibrous filter materials (FVM) with antibacterial properties, which are used for the manufacture of antibacterial filter elements (AFE) intended for equipment of personal respiratory protection equipment (PPE), in particular respirators against infections spread by airborne droplets .

There is a known method of obtaining an antimicrobial textile material, the essence of which is the processing of a modified cellulose-containing textile material from a grafted cellulose copolymer with an unsaturated monomer containing a quaternary ammonium group. The textile material is treated with an aqueous solution of an antimicrobial substance containing an acid group to obtain an antimicrobial material resistant to multiple washings.

Vyrnyk A.D., Penekzhik M.A., Shkurnikova I.S., Pismannyk K.D., Topchiev D.A., Kabanov V.A.,

Wittig F., Petzold G., Kappelt L., Heger A., Egger W., Wandrei H., Mo 3680225/28-05, appl. 27.10.1983). The disadvantage is that in this way antimicrobial properties can only be given to modified textile material, the production of which requires additional expenditure of money and time. The antimicrobial treatment itself is multi-stage and includes an additional washing stage, which also complicates the treatment technology.

A method of imparting antimicrobial properties to non-woven materials by immobilizing phenoxyethanol on the surface of cellulose fibers is known (Kozlyatnikova E.M., Gorchakova

V.M., Mzmailov B.A. Non-woven materials possessing biocidal properties.

Immobilization of phenoxyethanol on the fiber surface. Technology of the textile industry. 2010. Mo. 8 (329). P. 61-644. The disadvantage of this method is the use of an energy-intensive method of thermal modification using phenoxyethanol, which has strong allergenic and irritating properties.

There is also a known method of obtaining antibacterial fibrous material, which includes immobilization with 4-aminophenyleosulfonamide of the sulfonated form of the copolymer of styrene with divinylbenzene (Goropin V.M., Kremenchutskyi H.M., Burmistrov K.S., Shunkevich O.A.,

Polikarpov O.P. Synthesis and antibacterial properties of 4-aminophenylsulfonamide immobilized on a polymer carrier Pharmaceutical Journal. 2017. Me 1. S 5-10. OO

From 10.11603/2312-0967.2017.1.7525). The disadvantage of this method is the use of expensive and aggressive chlorosulfonic acid.

A known method of imparting antimicrobial properties to alginate fibers using wet spinning technology from an aqueous solution of sodium alginate and sulfonamide (Ma S., Pi

G., Naa MU., Sai U., Mao 9. Rabgisayop apa SNagasiegigayop oi Abzotbepi apa Apiibasiegia! Aidipaiye

Eireg5 I oadei myin ZipMapiiatide Rireg5 apa Roiutegv. 2015. MoI.16, M 6. R. 1255-1261. BOI 10.1007/5i2221-015-1255-7)Y. The disadvantage of this method is the use of a complex material manufacturing technology using a highly toxic and relatively low-effective antibacterial drug.

This method is chosen as the closest analogue.

The claimed useful model (method) coincides with the known method in the following set of essential features, namely: the use of aqueous solutions of M, 5-containing organic compounds with common structural fragments (the presence of an amine fragment and a sulfo group) and biological action (anti-staphylococcal activity) .

The basis of a useful model is the task of developing a method of providing FVM with antimicrobial properties, the use of which would allow simplifying the manufacturing technology using non-toxic compounds while simultaneously increasing the antibacterial effect.

The task set in the proposed useful model is achieved by impregnating the fibrous material with an aqueous solution of one of the non-toxic biocidal

BO aminomethanesulfonic acid (AM5A, 1), as well as its M-(2-hydroxyethyl)- (NEAM5A, 2), M-benzyl- (82AM5A, 3) and M-(tert-butyl)-(IVyAM5A, 4) derivatives.

What is new in the proposed useful model, unlike the closest analogue, is that instead of the technology of wet spinning from an aqueous solution of sodium alginate and sulfonamide, impregnation of the fibrous material with an aqueous solution of one of the non-toxic aminomethanesulfonic acids of the general formula ВМНОСНе5ОЗН, where В - H, is alkyl, is used.

The technical result is that the proposed method makes it possible to obtain FVM with higher biocidal properties using a simplified production technology thanks to the use of non-toxic aminomethanesulfonic acids of the general formula АМН!НОСН»оБОЗзН, where В -

H, alkyl. 60 Production of FVM was carried out as follows:

1 - pour the required amount of water into a container equipped with a stirrer, and then, while stirring, sequentially add an appropriate amount of one of the aminomethanesulfonic acids; 2 - a fibrous non-woven filter material, made, for example, of mylar fiber, is soaked for 10 minutes with the aqueous solution obtained according to item 1;

C - squeeze out, dry the material according to item 2 in the air at 20-30 "C of the obtained FVM, it is possible to manufacture AFE intended for equipping gas purification equipment, in particular, respirators - ZIZOD from airborne infections.

When studying the antimicrobial properties of FVM, the strain 5iarpuiosossiv atssheiv ATSS 25923 from the collection of the Museum of Microorganisms of the State University "Ukrainian Research Antiplague Institute named after I. Mechnikov" of the Ministry of Health of Ukraine (Odesa) was used, which is characterized by genetic stability and sensitivity to antibiotics and is used for quality control when determining the sensitivity of microorganisms to drugs | Order of the Ministry of Health of Ukraine dated 04/05/2007 Mo 167 "On the approval of methodological instructions "Determining the sensitivity of microorganisms to antibacterial drugs" 1. In addition, strains isolated from patients were used earnuiosossiv ashgeiv 2781 and earnuiosossiv ashgeiv Kunda from the collection of the Museum microorganisms of the State University "Institute of Microbiology and Immunology named after I. Mechnikov of the National Academy of Medical Sciences of Ukraine" (Kharkiv). The Kunda earpuosossivz acteiz strain was isolated from a patient after traumatic osteomyelitis and is multiresistant. The earpuiosossivz ashgeiv 2781 strain was isolated from a conjunctivitis patient and is moderately resistant to antibiotics.

In accordance with the task, the method of studying the specific activity of antimicrobial drugs in the modification of Kirby and Bauer using the standard method of Med discs was used. Microbiology / Ch. ed. Acad. YOU. Pokrovsky, Prof. OK.

Pozdeev - M.. GZOTAR Medicine, 1999. P. 160-164); Study of the specific activity of antimicrobial drugs / Volyanskyi Yu.L., Shirobokov V.P., Biryukova S.V. etc. //

Guidelines. - Kyiv. - 2004. - 38 p.|.

Petri dishes with Muller-Hinton agar were inoculated with a culture of microorganisms, introducing 0.6 ml of a diluted suspension of the pathogen. The suspension of the pathogen was prepared by diluting one-day cultures of microorganisms in the amount of (1.2-0.2)-1407 CFU/ml in accordance with the calculation based on the turbidity standard. After evenly distributing the dilution of the pathogen on the plate, the residues were removed and the prepared discs of FVM with the studied compounds were placed, sinking them into the surface of the agar. The cups were left in a thermostat at 37 C. After 18-20 hours, the zone of pathogen growth inhibition was determined around each of the discs. The research was carried out in 5 repetitions.

Example 1

Discs with FVM with a diameter of 0.5 cm, which contained fibrous filter material in its composition. The experimental versions of the discs contained the following compounds: AM5A (1),

NEAM5A (2), B2AM5A (3), IVNAM5A (4) and streptocid (5) (closest analogue) with the content of the active substance in the final concentration of 4.7-102 mmol/g. The results of the conducted research are given in the table. 1. According to the data presented in the table. 1, on the strain of Yetarpuiosossi5 aigei5

ATCC 25923, the use of none of the FVMs revealed inhibition of growth at a content of the tested substances on the fibrous carrier of 4.7102 mmol/g. As the closest analogue, discs with streptocide, prepared in a similar way, were used. The closest analogue did not show inhibitory activity in relation to all three studied strains of staphylococci. FVM--VIiAM5A showed a slight inhibitory effect relative only to Kunda's earnuiosossiv of 2.6 mm. FVM-AM5A and FVM-NEAMPF5A at a content of active substances of 4.7.1402 mmol/l showed a stable inhibitory effect against the strains earnuiosossiv ashgeiv5 2781 and arnuiosossiv ashgeiv Kunda, but the indicators of this inhibition were not significant (less than 10 mm). FVM-В2АМ5А also exhibited an inhibitory effect against both tested strains more than FVM-IVyAM5A, but less than FVM-AM5A and FVM-

NEAMP5A. To obtain more impressive results, you should increase the doses of the compounds that were part of the FVM.

Example 2

After obtaining preliminary results, similar discs with FVM with a diameter of 0.5 cm were prepared, which contained the studied compounds AM5A, NEAM5A, VpAM5A, IVAM5A and streptocid (the closest analogue) with the content of the active substance in a final concentration of 0.236 mmol/g. The obtained results are shown in the table. 2 and table. 3.

From the comparative table. It can be seen that all studied samples of SFVM based on aminomethanesulfonic acids 1-4 with their content had a higher level of inhibition of the growth of microorganisms relative to the strains 5. ashheiv 2781 and 5. ashheiv5 Kunda than the closest analog using streptocide (5).

On strain 5. ashgeiv 2781 FVM-AM5A and FVM-NEM5A even at the content of active substances of 4.7-10-2 mmol/l showed an inhibitory effect, which cannot be said about the closest analogue at this concentration. At a concentration of 0.236 mmol/g, FVM-NEM5A and FVM-

B2AM5A, although FVM-AM5A and FVM--VIAM5A at the same content also inhibited the growth of the studied strain 5. ashgeiv 2781 twice as much as the closest analog 5.

A similar trend was observed in relation to the b5.atssheiv Kunda strain. At a concentration of 4.7-102 mmol/g, all studied FVM inhibited the growth of this strain to a small extent, but the closest analogue 5 did not show any inhibitory effect at this concentration. FVM-Y-

ViAM5A with a content of 0.236 mmol/g of the active substance showed an inhibitory effect at the level of FVM based on streptocide, while FVM-AM5A, FVM-NEM5A and FVM-B2AM5A inhibited the growth of the studied strain 5.ashgei5 Kunda twice more than the closest analogue.

Relatively sensitive to antibiotics strain 5. ashteiv ATSS 25923 FVM with a content of 4.7-102 mmol/g of aminomethanesulfonic acids 1-4 did not show an inhibitory effect. At a content of 0.236 mmol/g, the growth of this strain was most inhibited by FVM based on the closest analogue, although FVM based on aminomethanesulfonic acid also inhibited its growth statistically significantly.

Thus, AM5A and its M-alkylated derivatives at a final concentration of 0.236 mmol/g, which were placed on FVM disks, inhibited the growth of all tested strains 5. ashgeiv5. In the case of antibiotic-resistant strains 5. ashgeiv5 2781 and 5. ashgeiz Kunda, the growth inhibition effect was higher than that of the reference streptocide preparation.

Based on the obtained results, it can be noted that the studied compounds - aminomethanesulfonic acid and its derivatives in a final concentration not lower than 0.236 mmol/g, can be used to obtain FVM with antibacterial properties.

Table 1

Received results 11 0 1 0 1 0 и1 0 1 0 ! 0 21 0 1 0 1 0 1 0 1 0 ! 0 of 0 1 0 1 0 1 0 1 0 |! 0 4 0 1 0 1 0 1 0 1 0 ! 0 5 0 1 ж жщжж0 1 0 и1 0 1 0 І 0 110 1 5 1 5 ІІ 5 | 5 | 4 o-o 21.0 1 5 1 B sh 8 | 5 | 7 | 50-14 with 0 1 0 1 5 HER 0 | 7 | hook 4 0 1 0 1 0 1 0 1 0 ! 0 5 0 1 B жщж0 1 0 и1 0 1 0 ! 0 110 1 0 1 5 HER 5 | 6 | 32132 21.0 1 0 1 5 | 5 | 6 | 32132 of 0 1 0 1 5 HER 5 | 0 | 202122 4 0 1 0 1 0 1 5 | 8 | 26:166 5 0 1 0 1 0 her1 0 her 0 ! 0

Table 2

The results obtained

The zone of inhibition of the growth of the strain biarpuiosossiv asheiv ATSS 25923 (mm) 1178 8 1 6 | 6 | 8 | 725049 21 12 | 6 her 8 | 6 | 8 | 8.0zh1.09 3 10 HER 4 1 6 | 8 | ЩЩЩ8 |and 4 6 | 70 her 0 1 8 | 10 | 685185 16,451.33

The zone of inhibition of the growth of the strain biarpuiosossisis acygeiv 2781 (mm) 11 lo ІІІ 8 1 6 ІІ 4 | щЩщфщЮКхВ8 17 2 1 71717115 | 6 1 71 | lov143 3 11 HER 713 1 8 | 6 | 12 | vine 4 10 HER 7 1 6 1 0 1 5 south 13 | comrade | 6 her 0 1 0 1 5 5yuzhsch Ko | 4 oz

The zone of inhibition of the growth of the biarpuiosossisis ashgeives Kunda strain (mm) 118 ІІ 70 | 6 | 8 | 6 | 760.75 21 10 1 8 1 70 | 70 | 6 | 88506 3 8 HER 8 1 6 | 8 | 4 | 68506 4 0 1 0 1 0 1 70 | 8 | 365223 5 10 HER 4 1 0 1 4 1 0 1 365183

Table C

The results of inhibition of the growth of strains 5. ashtey5 by the studied drugs 5. ashgey5 ATOS

Compounds, concentrations | 4.7-102 0.236 4.7.1072 0.236 4.74102 0.236 mmol/g| mmol/g MmMmolL/g mmol/g mmol/g mmol/g 11111110 | 72049 | 4-0 72-02 | 321.32 | 7,650.75 21111110 |8.0-1095| 5.0541.38 108-143) 32-1.32 | 8.8:0.8 81111111 1110 | 7202 | 24.5 100-513 | 20-1.22 | 6,850, 77741110 | вбвй85| 0 | 728 | 2b166 | 362 57777110 |tb4n33| 0 | 425294| 0 | sinuosity

Claims (1)

USEFUL MODEL FORMULA The method of imparting antibacterial properties to fibrous materials using an aqueous solution of the biocidal M, 5-containing organic compound, which differs in that the process is carried out by impregnating the fibrous material, and as a biocidal M, 5-containing organic compound, one of the aminomethanesulfonic acids of the general formula АМНОНе5БОЗН is used, where B - H, alkyl.