UA138612U - METHOD FOR PREDICTING THE DEVELOPMENT OF OSTEOARTHRITIS - Google Patents

Abstract

A method for predicting the development of osteoarthritis, in which a clinical examination, radiography. Additionally, in the patient's serum determine the polymorphism of genes of methylenetetrahydrofolate reductase (MTHFR C677T), nitric oxide synthase (eNOS T786C), the content of thrombomodulin, folic acid, glycosaminoglycans (GAG), 6-reactive and with a homozygous carrier of 677-TT, 786-CC and levels of thrombomodulin> 5 ng / ml, folic acid <6 ng / ml, GAG> 28 μmol / l, PSA> 6 mg / l, IL-6> 10 ng / l , homocysteine> 20 μmol / l predict the development of osteoarthritis. .

Description

The proposed useful model for predicting the development of osteoarthritis belongs to medicine, in particular to orthopedics and rheumatology. It is prescribed and can be used in diagnosing the development of osteoarthritis in the early stages.

Methods of predicting the development of osteoarthritis are known. These include x-rays, studies of biopsies of the synovial membrane, synovial fluid, and cartilage biopsies

I.N. Okorokov. Diagnosis of diseases of internal organs. M., 2001. - vol. 2. - pp. 186-196).

The disadvantage of these methods is the lack of the possibility of predicting the dynamics of the process in the early subclinical stages, preventing its development and possible complications due to the internal characteristics of the body.

The basis of a useful model is the task of developing a method that would allow predicting the development of osteoarthritis in the early stages.

The task is solved by the fact that, in addition to clinical examination and radiography, polymorphism of methylenetetrahydrofolate reductase genes (MTNEK) is determined in the patient's blood serum

Сб677Т), nitric oxide synthase (eМмО5з T786С), content of thrombomodulin, folic acid, glycosaminoglycans (GAG), C-reactive protein (CRP), interleukin 6 (IL-6), homocysteine. With homozygous carrier 677-TT, 786-SS, levels of thrombomodulin "5 ng/ml, folic acid "6 ng/ml, GAG "28 μmol/l, SRP 26 mg/l, IL-6 "10 ng/l, homocysteine 220 μmol/l predict the development of osteoarthritis.

Application of the method. Upon admission, the patient is examined, an X-ray is taken, the MTNEK C677tT, emo T786C gene polymorphism is determined, the content of thrombomodulin, folic acid, GAG, SRP, IL-b6, and homocysteine is determined by the immunoenzymatic method. With homozygous carrier 677-TT, 786-SS, levels of thrombomodulin "5 ng/ml, folic acid "6 ng/ml, GAG "28 μmol/l,

SRP »6 mg/l, IL-6 »10 ng/l, homocysteine 220 μmol/l predict the development of osteoarthritis.

A specific example of using the method.

Patient U., 64 years old, was admitted to the clinic due to pain in the left hip joint. Examined, X-ray taken. No signs of osteoarthritis were found. The polymorphism of MTNEK C677tT and emoz T786C genes was determined in blood serum. The content of thrombomodulin, folic acid, GAG, SRP, IL-6, and homocysteine was determined by immunoenzymatic method. A homozygous carrier of 677-TT and 786-SS was established, the levels of thrombomodulin - 12 ng/ml, folic acid - 2.6 ng/ml, GAG - 42 μmol/l, SRP - 19 mg/l, IL-6-24 ng/ l, homocysteine 33 μmol/l. The development of osteoarthritis was diagnosed. After 11 months, X-rays showed signs of osteoarthritis.

Thus, the proposed method is informative and allows diagnosing the development of osteoarthritis at an early stage.

Claims (1)

USEFUL MODEL FORMULA A method of predicting the development of osteoarthritis, which involves a clinical examination and radiography, which is distinguished by the fact that polymorphisms of the methylenetetrahydrofolate reductase (MINEV Sb677T) and nitric oxide synthase genes are additionally determined in the patient's blood serum (emoOo5 17860), the content of thrombomodulin, folic acid, glycosaminoglycans (GAG), C-reactive protein (CRP), interleukin 6 (IL-6), homocysteine and with homozygous carriers of 677-TT, 786-SS and levels of thrombomodulin »5 ng /ml, folic acid "6 ng/ml, GAG" 28 μmol/l, SRP "6 mg/l, IL-6 "10 ng/l, homocysteine 220 μmol/l predict the development of osteoarthritis.