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Application filed by Берінгер Маннхайм ГмбхfiledCriticalБерінгер Маннхайм Гмбх
Publication of UA12993ApublicationCriticalpatent/UA12993A/en
Measuring Or Testing Involving Enzymes Or Micro-Organisms
(AREA)
Abstract
The invention relates to the genetic engineering and relates to the obtainment of creatineamidinehydrolase, which is more stabile, than native enzyme, and therefore is more available for determination of creatinine content in serum, plasma or urine. The method for obtainment of creatineamidinehydrolase provides for construction of recombinant plasmid DNA, coding creatineamidinehydrolase, transformation of the obtained DNA of the strain of bacteria Escherichia coli, cultivation of the transformed strain, isolation and purification of the desired product.
UA4355715A1987-05-121988-05-11The method for obtainment of creatineamidinehydrolase
UA12993A
(en)
Cloning, sequencing and expression of a cDNA encoding bovine pancreatic deoxyribonuclease I in Escherichia coli: purification and characterization of the recombinant enzyme
Structural gene in transformed microbial host cell and structural gene coding various allele and maturity form of preprothaumatin recombined cloning vehicle
Comparative analysis of the effectiveness of C-terminal cleavage intein-based constructs in producing a recombinant analog of anophelin, an anticoagulant from Anopheles albimanus
Dna fragments, containing a lactic acid bacterium-specific¹regulator region for the expression of genes coding for¹normally heterologous proteins, recombinant plasmids based¹on such dna fragments, lactic acid bacteria which contain¹these recombinant plasmids and the heterologous proteins and¹products prepared with the aid of the above microorganisms