UA117474U - METHOD OF DETERMINATION OF BLOOD CHOLINESTERASIS ACTIVITY - Google Patents

METHOD OF DETERMINATION OF BLOOD CHOLINESTERASIS ACTIVITY

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Publication number
UA117474U
UA117474U UAU201700721U UAU201700721U UA117474U UA 117474 U UA117474 U UA 117474U UA U201700721 U UAU201700721 U UA U201700721U UA U201700721 U UAU201700721 U UA U201700721U UA 117474 U UA117474 U UA 117474U
Authority
UA
Ukraine
Prior art keywords
activity
blood
cholinesterasis
determination
rate
Prior art date
Application number
UAU201700721U
Other languages
Ukrainian (uk)
Inventor
Микола Євстахійович Блажеєвський
Олена Василівна Ковальська
Владислав Валерійович Дядченко
Original Assignee
Національний Фармацевтичний Університет
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Національний Фармацевтичний Університет filed Critical Національний Фармацевтичний Університет
Priority to UAU201700721U priority Critical patent/UA117474U/en
Publication of UA117474U publication Critical patent/UA117474U/en

Links

Abstract

Спосіб визначення активності холінестерази крові полягає у фотометричному вимірюванні швидкості ензимного гідролізу субстрата ацетилхоліну за його залишком у середовищі буферу з використанням індикатора на ацильну групу. Як індикатор на ацильну групу використовують n-фенетидин при рН 8,35, а швидкість ензимного гідролізу ацетилхоліну вимірюють за тангенсом кута нахилу лінійної ділянки кінетичної кривої за довжини хвилі 358 нм.A method for determining the activity of blood cholinesterase is to photometrically measure the rate of enzymatic hydrolysis of the acetylcholine substrate by its residue in the buffer medium using an acyl group indicator. As an indicator for the acyl group use n-phenetidine at pH 8.35, and the rate of enzymatic hydrolysis of acetylcholine measured by the slope of the angle of the linear section of the kinetic curve at a wavelength of 358 nm.

UAU201700721U 2017-01-26 2017-01-26 METHOD OF DETERMINATION OF BLOOD CHOLINESTERASIS ACTIVITY UA117474U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
UAU201700721U UA117474U (en) 2017-01-26 2017-01-26 METHOD OF DETERMINATION OF BLOOD CHOLINESTERASIS ACTIVITY

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
UAU201700721U UA117474U (en) 2017-01-26 2017-01-26 METHOD OF DETERMINATION OF BLOOD CHOLINESTERASIS ACTIVITY

Publications (1)

Publication Number Publication Date
UA117474U true UA117474U (en) 2017-06-26

Family

ID=59092958

Family Applications (1)

Application Number Title Priority Date Filing Date
UAU201700721U UA117474U (en) 2017-01-26 2017-01-26 METHOD OF DETERMINATION OF BLOOD CHOLINESTERASIS ACTIVITY

Country Status (1)

Country Link
UA (1) UA117474U (en)

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