UA117829U - METHOD OF DETERMINATION OF BLOOD CHOLINESTERASIS ACTIVITY - Google Patents

METHOD OF DETERMINATION OF BLOOD CHOLINESTERASIS ACTIVITY

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Publication number
UA117829U
UA117829U UAU201700717U UAU201700717U UA117829U UA 117829 U UA117829 U UA 117829U UA U201700717 U UAU201700717 U UA U201700717U UA U201700717 U UAU201700717 U UA U201700717U UA 117829 U UA117829 U UA 117829U
Authority
UA
Ukraine
Prior art keywords
activity
determination
blood
cholinesterasis
enzymatic hydrolysis
Prior art date
Application number
UAU201700717U
Other languages
Ukrainian (uk)
Inventor
Микола Євстахійович Блажеєвський
Олена Василівна Ковальська
Владислав Валерійович Дядченко
Original Assignee
Національний Фармацевтичний Університет
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Національний Фармацевтичний Університет filed Critical Національний Фармацевтичний Університет
Priority to UAU201700717U priority Critical patent/UA117829U/en
Publication of UA117829U publication Critical patent/UA117829U/en

Links

Abstract

Спосіб визначення активності холінестерази крові полягає у фотометричному вимірюванні швидкості ензимного гідролізу субстрата ацетилхоліну за його залишком у середовищі буферу з використанням індикатора на ацетильну групу. Як індикатор на ацетильну групу використовують 3,3',5,5'-тетраметилбензидин, реакцію ензимного гідролізу та визначення субстрату проводять при рН 8,3, а швидкість ензимного гідролізу ацетилхоліну вимірюють через 10 хв.A method for determining the activity of blood cholinesterase is to photometrically measure the rate of enzymatic hydrolysis of the acetylcholine substrate by its residue in the buffer medium using an indicator for the acetyl group. As an indicator for the acetyl group used 3,3 ', 5,5'-tetramethylbenzidine, the reaction of enzymatic hydrolysis and determination of the substrate is carried out at pH 8.3, and the rate of enzymatic hydrolysis of acetylcholine is measured after 10 minutes.

UAU201700717U 2017-01-26 2017-01-26 METHOD OF DETERMINATION OF BLOOD CHOLINESTERASIS ACTIVITY UA117829U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
UAU201700717U UA117829U (en) 2017-01-26 2017-01-26 METHOD OF DETERMINATION OF BLOOD CHOLINESTERASIS ACTIVITY

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
UAU201700717U UA117829U (en) 2017-01-26 2017-01-26 METHOD OF DETERMINATION OF BLOOD CHOLINESTERASIS ACTIVITY

Publications (1)

Publication Number Publication Date
UA117829U true UA117829U (en) 2017-07-10

Family

ID=59266462

Family Applications (1)

Application Number Title Priority Date Filing Date
UAU201700717U UA117829U (en) 2017-01-26 2017-01-26 METHOD OF DETERMINATION OF BLOOD CHOLINESTERASIS ACTIVITY

Country Status (1)

Country Link
UA (1) UA117829U (en)

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