UA114171C2 - Herbicide tolerant soybean plants and methods for identifying same - Google Patents

Abstract

The invention provides specific transgenic soybean plants, plant material and seeds, characterized in that these products harbor a stack of specific transformation events at specific locations in the soybean genome. Tools are also provided which allow rapid and unequivocal identification of these events in biological samples.

Description

EOM sho i (c) Not "Bolota tin r" vve teor" flanking fragment Foreign DNA Identification Fragment pos o me: POS o meZ certain popy Mn nia " iskkkzhikyktzhiknn and i t h

ME A otsov i E Ks U o postlyutm? 4 5 years)

Cross-references to family applications

The original application claims priority under the prior US patent application

Mo 61/367251, submitted on July 23, 2010; preliminary US patent application No. 61/263707, filed on November 23, 2009; and prior European patent application No. EP 09014565.7, filed on November 23, 2009, the contents of each of which are fully incorporated herein by reference.

The field of technology

The present invention relates to transgenic plants, plant material and soybean seeds, characterized by the content of at least two specific transformation events, in particular, the presence of at least two sets of genes encoding proteins that confer resistance to herbicides, each of which is characterized by a specific position in the soybean genome. Soybean plants of the invention combine a herbicide resistance phenotype with agronomic performance, genetic stability and functionality in different genetic environments equivalent to an untransformed soybean line in the absence of herbicide(s). In addition, the present invention provides methods and kits for identifying the presence of plant material, including specialized transformation events EE-SMZ3 and EE-SM2 or EB-OMI'I, in biological samples.

Technical level

Phenotypic expression of a transgene in plants is determined both by the structure of the gene or genes themselves, and by its or their position in the plant genome. At the same time, the presence of transgenes or "foreign DNA" in different positions within the genome affects the overall phenotype of the plant in different ways. Agronomically or industrially, the successful incorporation by genetic manipulation into the organism of a plant of a trait of commercial interest, depending on various factors, can be a lengthy procedure. The actual transformation and regeneration of the genetically transformed plant is only the first stage of selection, which includes extensive research on genetic characteristics, crossing and field trials, ultimately leading to the selection of an elite event.

The importance of unambiguous identification of an elite event is increasing in light of the discussion of new food products/feeds, distribution of products based on genetically modified and

From unmodified organisms and identification of patented materials. Ideally, this method of identification is both quick and simple, not requiring large-scale laboratory equipment. In addition, this method should provide results that allow an unambiguous identification of an elite event without expert interpretation, however, by necessity, withstand expert evaluation. Specialized tools for identification of elite events EE-

CM and EE-OM'I1 or EE-CM2 in biological samples are described in this application.

In the present invention, EE-CM3 was identified as an elite event from a population of transgenic soybean plants in the development of herbicide-resistant cultivar soybean (CiIusipe tah) comprising a gene encoding resistance to glyphosate in combination with a gene conferring resistance to 4- hydroxyphenylpyruvate dioxygenases (HRPOB), each of which is under the control of a promoter that ensures expression in a plant cell.

EB-OMI1 and EE-OM2 were previously identified as elite events from a population of transgenic soybean plants in the development of herbicide-resistant soybean (Siusipe tah) that includes a gene encoding glufosinate resistance under the control of a promoter that ensures expression in plant cell, and described in applications M/02006/108674 and УМ02006/108675 (both publications are incorporated into this document by reference).

Soybean plants containing a gene for resistance to herbicides have been described in the literature. However, none of the known publications report this invention.

It is known in the art that obtaining a commercial elite transformation event of herbicide-resistant soybean plants with acceptable agronomic performance without yield degradation and sufficient resistance to three different classes of herbicides is far from an easy task.

Indeed, it was reported that the first soybean event (event 40-3-2) to enter the market as resistant to herbicides was characterized by a significant deterioration in yield compared to isogenic or nearly isogenic lines (EiYitoge ei ai. (2001) Adgop. u. 93:408-412).

In addition, a soybean Oripyt'"mMm SZAT'M (event 356043) was bred that combined glyphosate resistance with resistance to acetolactate synthase herbicides, however, this soybean was reported not to meet the standards for glyphosate resistance by itself (except in combination with another event of soybean resistance to glyphosate (for example, event 40-3-2) (see, for example, mlym/.riootregod.sot/arrz/pemv "rid-pemzagspimevvid-ad4i OPNU9MKMUE)). because the essence of the invention

The present invention relates to transgenic soybean plants or their seeds, cells and tissues containing an expression cassette stably integrated into their genome, including a herbicide resistance gene, including the coding sequence of the 2p71ERZRB gene, and another herbicide resistance gene, including the coding sequence of NRPO-RE MU336 (according to the description in Example 1.1 of this application and as presented in 5EO IU Mo 1), as well as a second expression cassette including the herbicide resistance gene containing the coding sequence, phosphinothricin acetyltransferase in accordance with the description in Example 1 of this application and as presented in ZEO IO Mo 11. Transgenic soybean plants or their seeds, cells or tissues are resistant to herbicides based on glyphosate, glufosinate and the HPRO inhibitor herbicide, for example, isoxaflutol, and in the absence of herbicides - have agronomic performance, to a large extent equivalent to the performance of a non-transgenic isogenic line. After application of one or more herbicides to which the plant is resistant, it exhibits an agronomic phenotype superior to that of the non-transgenic plant.

According to the data of the invention, soybean plants or their seeds, cells or tissues contain the elite event EE-CM3Z and EE-CMI1 or EE-CM3Z and EE-CM2.

In particular, the present invention relates to transgenic soybean plants, their seeds, cells or tissues, the genomic DNA of which is characterized by the fact that its analysis, according to the PCR-identification protocol, as described here, using two primers to the 5'- or 3'- - the flanking area

EE-OM3Z and foreign DNA including herbicide resistance genes in EE-CM-3, respectively, detects a fragment specific for EE-SMUZ, and in addition, analysis, according to the PCR-identification protocol, as described here, with using two primers to the 5'- or 3'-flanking region

EBE-OMI1 or EB-CM2 and foreign DNA, which includes genes for resistance to glufosinate, respectively, reveals a fragment specific for EE-Z3M1 or EE-CM2. Target primers for detection

EE-CM3Z may be a 5'-flanking region within 5EO IO Mo: 2 and foreign DNA including herbicide resistance genes, respectively. Target primers for the detection of EE-CM3, for example, primers that include or consist (mainly) of the nucleotide sequence of ZEO

IO Mo: 5 and BEO IO Mo: 4 or 5EO IOI Mo.: 7, respectively, there may also be a 3'-flanking region within 5EO IU Mo: C and foreign DNA including herbicide resistance genes, respectively, and primers detect a DNA fragment with a size of 100 to 800 bp, for example, a fragment

With a size of approximately 263 p.o. or approximately 706 p.o. Target primers for detection of EE-

OMI1 can be the 5'--flanking region within ZEO IU Mo: 12 and foreign DNA including herbicide resistance genes, respectively. The target of primers for the detection of EE-CM'I1, for example, primers that include or consist (mainly) of the nucleotide sequence of HEO

IO Mo: 16 and ZEO IO Mo: 17, respectively, can also be the 3'-flanking region within ZEO IU

Mo: 13 and foreign DNA comprising herbicide resistance genes, respectively, and the primers detect a DNA fragment of 100 to 500 bp, for example, a fragment of approximately 183 bp. The target of primers for detection of EE-ZM2 can be the 5'-flanking region within the 5EO IJOO Mo: 14 and foreign DNA that includes genes for resistance to herbicides, respectively. The target of primers for detection of EE-ZM2, for example, primers that include or consist (mainly) of the nucleotide sequence ZEO IU Mo: 18 and BEO IO Mo: 19, respectively, can also be a 3'-"linking region within 5EO IO Mo: 15 and foreign DNA comprising herbicide resistance genes, respectively, wherein the primers detect a DNA fragment of 100 to 500 bp, for example, a fragment of approximately 151 bp.

The reference seed containing the elite event EE-OM3, according to the invention, was deposited in

МСИМВ under the accession number МСИМВ 41659. The reference seed containing the elite event EE-СМ'I, according to the invention, is deposited in the МСИМВ under the accession number МСИМВ 41658. The reference seed containing the elite event EE-ЗМ2, according to the invention, is deposited in МСИМВ under the accession number МСИМВ 41660. The reference seed containing the elite event EE-СМЗ and EE-СМ'И1 was deposited in the ATCC under the accession number РТА-11041. The reference seed containing the elite event EE-OM3Z and EE-SM2 was deposited in ATCC under the accession number PTA-11042.

One of the variants of the embodiment of the invention is a seed containing an elite event EE-MZ (a reference seed containing the specified event and deposited in the МСИМВ under the access number

МСИМВ 41659), and, in addition to that containing the elite event EE-ZМИ1 (reference seed containing the specified event and deposited in МСИМВ under accession number МСИМВ 41658), or seeds containing the event EE-ЗМ3З and EE-ОМИ1 (reference seed containing the specified events and deposited in

ATCC accession number PTA-11041) that grow in soybean plants resistant to at least three herbicides, in particular, resistant to glyphosate and/or HPRO inhibitors, such as isoxaflutol, and/or glutamine synthase inhibitors, such as glufosinate.

Another embodiment of the invention is a seed containing the elite event EE-60 СМЗ3 (a reference seed containing the specified event and deposited in the Moscow State University under accession number Moscow State University 41659), and, in addition, containing the elite event EE-СМ2 ( the reference seed containing the specified event and deposited in the Moscow State University of Agricultural Sciences under the accession number of the Moscow State University 41660), or the seed containing the event EE-ZMZ and EE-OM2 (the reference seed containing the specified events and deposited in the ATCC under the accession number PTA-11042) , which grow in soybean plants, are resistant to at least three herbicides, in particular, resistant to glyphosate and/or HPRO inhibitors, such as isoxaflutol, and/or glutamine synthase inhibitors, such as glufosinate.

The seed deposited in the CSIMP under the CSIMP accession number 41659 is a seed lot consisting of at least about 95% of transgenic seed containing the elite event EE-CM3, which grows into herbicide-resistant plants, and these plants are resistant to glyphosate and/or isoxaflutol. Seed or seed progeny obtained or derived from deposited seed (e.g., after crossing with other soybean plants with a different genetic background) can be sown and the growing plants treated with glyphosate or HRROB inhibitors such as isoxaflutol as described herein to produce plants resistant to glyphosate or isoxaflutol containing elite event EE-OM3. The seed deposited in the CSIMB under accession numbers CSIMB 41658 and CSIMB 41660 are seed lots consisting of at least approximately 95 95 transgenic seed containing elite events EE-ZMI1 or

EBE-OM2, respectively, which grow in plants resistant to herbicides, and these plants are resistant to glufosinate. These seeds can be sown and the growing plants treated with glufosinate to produce glufosinate-resistant plants containing elite events EE-5M1 or

EE-OM2.

The seed deposited in the ATCC under the accession number PTA-11041 is a seed lot consisting of at least about 95% transgenic seed containing the elite event EE-CM3Z and the elite event EE-ZMI1 in homozygous form, which grows into plants, resistant to herbicides, and these plants are resistant to glyphosate, glufosinate and/or isoxaflutol.

Seed or seed progeny obtained or derived from deposited seed (eg, after crossing with other soybean plants with a different genetic background) can be sown and the growing plants treated with glyphosate, glufosinate, or HPRO inhibitors, such as isoxaflutol, as described herein to produce plants, resistant to glyphosate, glufosinate or isoxaflutol containing the elite event EE-ZMZ and EE-SMI1.

The seed deposited in the ATCC under accession number PTA-11042 is a seed lot consisting of at least about 95% transgenic seed containing the elite event EE-CM3Z and the elite event EE-ZM2 in homozygous form, which grows into plants, resistant to herbicides, and these plants are resistant to glyphosate, glufosinate and/or isoxaflutol.

Seed or seed progeny obtained or derived from deposited seed (e.g., after crossing with other soybean plants with a different genetic background) can be sown and the growing plants treated with glyphosate, glufosinate, or HPRO inhibitors, such as isoxaflutol, as described herein to produce plants, resistant to glyphosate, glufosinate or isoxaflutol, containing elite event EE-ZMZ and EE-CsM2.

The present invention further relates to cells, tissues and progeny of a plant containing the elite event EE-CM3Z and, in addition, containing the elite event EE-CM'I, which can be obtained by growing a plant containing the elite event EE- CM3Z and EE-OMI1, deposited in ATSS as

PTA-11041, or growing a plant containing the elite event EB-CM3 from seeds deposited in the МСИМВ under accession number МСИМВ 41659, and crossing the specified plant with a plant containing the ЕЕ-СМ1 elite event grown from seeds deposited in the МСИМВ under accession number МСИМВ 41658. The present invention also relates to cells, tissues and progeny of a plant containing the elite event EE-SMZ and, in addition, containing the elite event EE-OM2, which can be obtained by growing a plant containing the elite event EE -SMZ and EE-SM2 deposited in ATCC as PTA-11042, or growing a plant containing an elite event EE-

CM3Z, from the seeds deposited in the МСИМВ under the accession number МСИМВ 41659, and the crossing of the specified plant with a plant containing the elite event EE-M2, grown from the seeds deposited in the МСИМВ under the accession number МСИМВ 41660. This invention, in addition, relates to plants obtainable from (e.g., by propagation and/or crossing with) a soybean plant or seed as described immediately before.

The present invention also relates to soybean plants containing the elite event EE-OM3Z and EE-SMI1 or

EE-CM3Z and EE-CM2.

The present invention, in addition, relates to a method of identification of a transgenic plant or its cells or tissues containing the elite event EE-ZMZ3 and EB-SOMI or EB-SMZ and EB-OSM2, based on the identification of the presence of characteristic DNA sequences or amino acid sequences, which are encoded by such DNA sequences of the transgenic plant, cells or 60 tissues. According to the best version of the embodiment of this invention, the following characteristic sequences

DNA is a sequence with a length of at least 15 bp, 15 bp, at least 20 bp, 20 bp. or 30 bp or more containing event insertion sites, i.e. both a fragment of inserted foreign DNA containing herbicide resistance genes and a fragment of the soybean genome (5'- or 3-flanking region) joined to each other , which makes it possible to specifically identify an elite event.

This invention, in addition, relates to methods of identification of elite events EE-ZMZ and EE-

SMI1 or elite events EE-ZMZ3 and EE-ZM2 in biological samples, methods based on the use of primers or probes that specifically recognize the 5'- and/or 3-flanking sequence of foreign DNA in EE-5MZ3 and EE-SMI or EE- OM3 and EE-COM2, which includes herbicide resistance genes.

In particular, the present invention relates to a method that includes the amplification of two nucleotide sequences present in biological samples using two polymerase chain reactions and at least four primers, and the first primer recognizes the 5'- or 3'-flanking region of foreign DNA in the EE -CM3, which includes herbicide resistance genes, the second primer recognizes the sequence within the foreign DNA in EE-OM3, which includes herbicide resistance genes, the third primer recognizes the 5'- or 3'-flanking region of foreign DNA in EE-СМИ1 or EE-CM2, which includes herbicide resistance genes, and the fourth primer recognizes a sequence within the foreign DNA in EE-ZMI1 or EE-OM2, which includes herbicide resistance genes, preferably to obtain two DNA fragments of 100 to 800 bp in size . Primers for the identification of EE-M3 can recognize the sequence within the 5'-flanking region of EE-CM3Z (5EO IO Mo 2, from position 1 to position 1451) or within the 3'-flanking region of EE-2M3 (complementary 5EO IUO Mo Z from position 241 to position 1408) and a sequence within the foreign DNA that includes herbicide resistance genes (complementary 5EO IU Mo 2 from position 1452 to 1843 or 5EO IUO Mo C from position 1 to position 240, or 5EO IUO Mo 20 from position 1452 to provision 16638, or its complementary chain), respectively. A primer recognizing the 3′ flanking region may include the nucleotide sequence 5EO IO Mo 5, and a primer recognizing a sequence within the foreign DNA that includes herbicide resistance genes may include the nucleotide sequence 5EO IUO Mo 4 or ZEO IO Mo: 7 described here. Primers for identification of EE-C:M1

Zo can recognize the sequence within the 5'-flanking region of EE-ZM1 (5EO IJUO Mo 12, from position 1 to position 209) or within the 3'-flanking region of EE-OM1 (complementary

ZEO IU Mo 13 from position 569 to position 1000) and the sequence within the foreign DNA, which includes the gene for resistance to the herbicide EE-OMI1 (complementary 5EO IU Mo 12 from position 210 to position 720 or 5EO IU Mo 13 from position 1 to position 568 ), respectively.

A primer recognizing the 5'-flanking region of EBE-OMI may include the nucleotide sequence 5EO IO Mo. 16, and a primer that recognizes the sequence within foreign DNA

EB-OMI, may include the nucleotide sequence 5EO IYUO Mo.: 17 described herein. Primers for the identification of EE-SU0M2 can recognize the sequence within the 5'-flanking region of EE-

CM2 (5EO IYUO Mo. 14, from position 1 to position 311) or within the 3'-flanking section

EBE-OM2 (complementary 5EO IO Mo 15 from position 508 to position 1880) and the sequence within the foreign DNA, including the herbicide resistance gene EE-OMI1 (complementary ZEO

IO Mo 14 from position 312 to position 810 or 5EO IU Mo 15 from position 1 to position 507), respectively. A primer that recognizes the 3'-flanking region of EE-ZM2 can include the nucleotide sequence ES IO Mo 18, and a primer that recognizes the sequence within the foreign DNA of EE-OM2 can include the nucleotide sequence zhEO IJUO Mo 19 described here.

PCR amplification can be performed simultaneously or sequentially.

In particular, the present invention relates to a method of identifying the elite event EE-ZM3 and EE-M1 in biological samples, and this method includes the amplification of at least two nucleotide sequences present in the biological sample by means of a polymerase chain reaction using two primers that include or consisting (mainly) of the nucleotide sequence ЗЕО ИО Мо 4 и 5EO ИХО Мо 5, respectively, resulting in a DNA fragment approximately 263 bp long, or two primers including or consisting (mainly) of the nucleotide sequence ZEO

IO Mo 5 and 5BEO IO Mo 7, respectively, resulting in a DNA fragment approximately 706 bp long, and polymerase chain reaction using two primers that include or consist (mainly) of the nucleotide sequence BEO IUO Mo 16 and ZEO

IO Mo 17, respectively, resulting in a DNA fragment approximately 183 bp long. These two polymerase chain reactions can be performed simultaneously or sequentially.

In particular, the present invention relates to a method of identifying the elite event EE-ZM3 and EE-CM2 in biological samples, and this method includes the amplification of at least two nucleotide sequences present in the biological sample by means of a polymerase chain reaction using two primers, which include or consist (mainly) of the nucleotide sequence ЗЕО ИО Мо 4 и 5ЕО ИХО Мо 5, respectively, resulting in a DNA fragment approximately 263 bp long, or two primers that include or consist (mainly) of the nucleotide sequence ZEO sequences

IO Mo 5 and 5BEO IO Mo 7, respectively, resulting in a DNA fragment approximately 706 bp long, and polymerase chain reaction using two primers that include or consist (primarily) of the nucleotide sequence BEO IUO Mo 18 and ZEO

IO Mo 19, respectively, resulting in a DNA fragment approximately 151 bp long. These two polymerase chain reactions can be performed simultaneously or sequentially.

The present invention further relates to the specific flanking sequences of EE-CM3Z described herein, in combination with the specific flanking sequences of EE-OMI, which can be used to develop specific methods for identifying the simultaneous presence of EE-

CM3Z and EE-OMI in biological samples. Such combined specific flanking sequences can also be used as reference control material in identification assays.

In particular, the present invention relates to the 5'- and/or 3'-(flanking regions of EE-CM3Z in combination with the 5- and/or 3-flanking regions of EE-SZM'I, which can be used to design specific primers and probes as described further herein In addition, nucleic acid molecules, preferably approximately 150-850 bp in length, comprising a sequence that can be amplified with primers comprising or consisting (mainly) of the nucleotide sequence cHEO can also be used as reference material IYUO Mo 7 and ZEO IYU Mo 5 or

ZEO IU Mo 4 and 5EO IU Mo 5, in combination with nucleic acid molecules including a sequence that can be amplified using primers including or consisting (mainly) of the nucleotide sequence ZEO IU Mo 16 and 5EO IUO Mo 17; in particular, such nucleic acid molecules are obtained when using such primers in material including EE-CM3Z and EE-CMI1.

The present invention, in addition, also relates to specific flanking sequences

EBE-OM3 described here, in combination with a specific flanking sequence of EE-OM2, which can be used to develop specific methods for the identification of simultaneous

From the presence of EE-ZMZ and EE-CM2 in biological samples. Such combined specific flanking sequences can also be used as reference control material in identification assays. Namely, the present invention relates to the 5'- and/or 3'-flanking regions of EE-OM3 in combination with the 5- and/or 3-flanking regions of EE-ZM2, which can be used to design specific primers and probes according to the following In addition, nucleic acid molecules, preferably about 150-850 bp in length, comprising a sequence that can be amplified with primers comprising or consisting (primarily) of the nucleotide sequence of zEO can be used as reference material. IU Mo 7 and 5EO IU Mo 5 or 5EO IUO Mo 4 and 5EO IO Mo 5, in combination with nucleic acid molecules that include a sequence that can be amplified using primers that include or consist (mainly) of the nucleotide sequence ZEO IUO Mo 18 and 5EBO IO Mo 19, in particular, such nucleic acid molecules are obtained when using such primers in material including EE-CM3Z and EE-CM2.

The present invention, in addition, relates to methods of identifying the simultaneous presence of EE-

CM3Z and EE-SOSMI in biological samples based on the use of such specific primers or probes. Primers for detecting EE-5M3 may include, consist of, or consist primarily of a nucleotide sequence of 17 to about 200 consecutive nucleotides in length, which is selected from the nucleotide sequence of ZEO IU Mo 2, from nucleotide 1 to nucleotide 1451, or the complementary strand of nucleotide sequence 560 IU 3, from nucleotide 241 to nucleotide 1408, in combination with primers including, consisting of, or consisting mainly of a nucleotide sequence of from 17 to about 200 consecutive nucleotides in length, selected from the nucleotide sequence of ZEO IU

Mo 1, for example, a nucleotide sequence with a length of from 17 to approximately 200 consecutive nucleotides, which is selected from the complementary chain of the nucleotide sequence of ZEO IU Mo 2, from nucleotide 1452 to nucleotide 1843, or the nucleotide sequence of ZEO IU Mo 3, from nucleotide 1 to nucleotide 240. Primers for the detection of EE-MDI can include, consist of, or consist primarily of a nucleotide sequence of 17 to about 200 consecutive nucleotides in length, which is selected from the nucleotide sequence of ЭО ЮЮ Mo 12, from nucleotide 1 to nucleotide 209, or the complementary strand of the nucleotide sequence of FEO IU 13, from nucleotide 569 to nucleotide 1000, in combination with primers 60 comprising, consisting of, or consisting mainly of a nucleotide sequence of 17 to about 200 consecutive nucleotides in length, which is selected from the nucleotide sequence of ZEO IO Mo 11, for example, nucleotide sequences ranging in length from 17 to about 200 consecutive nc leotides that are selected from the complementary chain of the nucleotide sequence ZEO IUO Mo 12, from nucleotide 219 to nucleotide 720, or the nucleotide sequence 5EO IUO Mo 13, from nucleotide 1 to nucleotide 568. Primers can also include the specified nucleotide sequences located directly on their 3' "-ends, and, in addition, include unrelated sequences or sequences that are derived from the indicated nucleotide sequences, but contain inconsistencies.

The present invention, in addition, relates to methods of identifying the simultaneous presence of EE-

SM and EE-OMEO in biological samples based on the use of such specific primers or probes. Primers for detection of EE-5M3 can include, consist of, or mainly consist of a nucleotide sequence with a length of from 17 to about 200 consecutive nucleotides in accordance with the description in the previous paragraph. Primers for detecting EE-YAM2 can include, consist of, or consist primarily of a nucleotide sequence of 17 to about 200 consecutive nucleotides in length, which is selected from the nucleotide sequence 5EO IO Mo 14, from nucleotide 1 to nucleotide 311, or the complementary strand of the nucleotide sequence 560 IU Mo 15, from nucleotide 508 to nucleotide 1880, in combination with primers including, consisting of, or consisting mainly of a nucleotide sequence of from 17 to about 200 consecutive nucleotides in length, which is selected from the nucleotide sequence zEO IUO Mo 11, for example, nucleotide sequences with a length of from 17 to about 200 consecutive nucleotides, which are selected from the complementary strand of the nucleotide sequence ZEO IU Mo 14, from nucleotide 312 to nucleotide 810, or the nucleotide sequence 5EO IUO Mo 15, from nucleotide 1 to nucleotide 507. Primers can also include the specified nucleotide sequences located directly at their 3'-end, and, in addition, include unrelated sequences or sequences that are derivatives of the specified nucleotide sequences, but contain mismatches.

This invention, in addition, refers to sets for identification of elite events EE-OMZ and

EBE-OMI in biological samples including at least one primer or probe that specifically recognizes the 5- and/or 3'-linking sequence of foreign DNA in EE-ZM3, which

Zo includes genes for resistance to herbicides, and at least one primer or probe that specifically recognizes the 5'- and/or 3'-flanking sequence of foreign DNA in EE-OM'I, which includes genes for resistance to herbicides.

This invention, in addition, refers to sets for identification of elite events EE-OMZ and

EBE-OM2 in biological samples comprising at least one primer or probe that specifically recognizes the 5- and/or 3'-(flanking sequence of foreign DNA in EE-OM3 that includes herbicide resistance genes, and at least one primer or probe that specifically recognizes the 5'- and/or 3'-flanking sequence of foreign DNA in EE-CM2, which includes the herbicide resistance gene.

Kits according to the invention can include, in addition to the primer that specifically recognizes the 5- or 3-flanking sequence of EE-ZMZ3 and the 5- or 3-flanking sequence of EE-CM'I, an additional primer that specifically recognizes the 5'- and/ or a foreign 3'-linking sequence

DNA in EE-OM3 comprising the herbicide resistance genes and an additional primer specifically recognizing a sequence within the foreign DNA in EE-ZOM comprising the herbicide resistance genes for use in accordance with the PCR identification protocol.

Kits according to the invention may also include, in addition to a primer, that specifically recognizes the 5'- or 3'-flanking sequence of EE-ZMZ and the 5'- or 3'-flanking sequence of EE-

OM2, an additional primer that specifically recognizes the 5'- and/or 3'-flanking sequence of the foreign DNA in EE-CM3, which includes herbicide resistance genes, and an additional primer that specifically recognizes the sequence within the foreign DNA in EE-OM2 , which includes herbicide resistance genes, for use according to the PCR-identification protocol.

A primer that recognizes the 3-flanking region of EE-ZM3 can include the nucleotide sequence 5EO IO Mo 5, and a primer that recognizes foreign DNA transgenes in EE-CM3 that includes herbicide resistance genes can include the nucleotide sequence ZEO IU Mo 4 or 7, or any other primer or combination of primers for detection of EE-CM3 as described herein, in combination with a primer recognizing the 5'-linking region of EE-CM1, which may include the nucleotide sequence 5EO IU Mo 16, and a primer, which recognizes transgenes of foreign DNA in EE-OMI1, which includes genes for resistance to herbicides, may include the nucleotide sequence ZEO IU Mo 17.

A primer that recognizes the 3-flanking region of EE-ZM3 can include the nucleotide sequence 5EO IO Mo 5, and a primer that recognizes foreign DNA transgenes in EE-CM3 that would include herbicide resistance genes can include the nucleotide sequence ZEO IU Mo 4 or 7, or any other primer or combination of primers for detection of EE-CM3 as described herein, in combination with a primer that recognizes the 5'-linking region in EE-BM2, which may include the nucleotide sequence 5EO IU Mo 18, and a primer that recognizes transgenes of foreign DNA in EE-OM2, which includes genes for resistance to herbicides, may include the nucleotide sequence of ZEO IU Mo 19.

This invention, in addition, relates to a set for identification of elite events EE-SOMZ and

EBE-OMI in biological samples, including primers for PCR including or consisting (mainly) of the nucleotide sequence 5BEO IU Mo 5 and BEO IU Mo 4, and primers for PCR including or consisting (mainly) of the nucleotide sequence FEO IU Mo 16 and

ZEO IU Mo 17, for use in PCR-based identification of EE-KHMZ/EE-OMI.

This invention, in addition, relates to a set for identification of elite events EE-SOMZ and

EBE-OM2 in biological samples, which includes primers for PCR, including or consisting (mainly) of the nucleotide sequence 5EO IO Mo 5 and BEO IO Mo 4, and primers for PCR including or consisting (mainly) of the nucleotide sequence FEO IU Mo 18 and

ZEO IU Mo 19, for use in PCR-based identification of EE-AMZ/EE-OM2.

The present invention also relates to a kit for the identification of the elite event EE-M3Z and EE-CM1 in biological samples, comprising two specific probes, the first of which includes or consists (mainly) of a sequence corresponding to (or complementary to) a sequence having 80 95 to 100 95 identity with the EE-ZM3 specific region, and the second includes or consists (primarily) of a sequence corresponding to (or complementary to) a sequence having 8095 to 10095 identity with the EE-C2MI specific region. In the best case, the sequence of the first probe corresponds to a specific region that includes part of the 5'- or 3'-flanking region of EE-CM3, and the second probe corresponds to a specific region that includes part of the 5'- or 3'-flanking region of EE-ZM1 . In the most preferred case, the EE-74MZ3-specific probe includes or consists (mainly) of (or is complementary to) a sequence having 8095 to 10095 identity with the sequence located between nucleotides 1441-1462 of 5EO IO Mo 2 or a sequence having from 80 95 to 100 95 of identity with the sequence located between nucleotides 220-260 of IO Mo 3, and EE-

The CM1-specific probe includes or consists (mainly) of (or is complementary to) a sequence having 8095 to 10095 identity with the sequence located between nucleotides 199-220 5EO IO Mo 12, or a sequence having 80 95 to 100 95 identity with the sequence located between nucleotides 558-579 of 5EO IU Mo 13.

The present invention also relates to a kit for the identification of the elite event EE-CM3Z and EE-OSM2 in biological samples, comprising two specific probes, the first of which includes or consists (mainly) of a sequence corresponding to (or complementary to) a sequence having 80 95 to 100 95 of identity with the specific region of EE-ZM3, and the second includes or consists (mainly) of a sequence corresponding to (or complementary to) a sequence having 8095 to 10095 of identity with the specific region of EE-ZM2. Preferably, the sequence of the first probe corresponds to a specific region that includes part of the 5'- or 3'-flanking region of EE-CM3, and the second probe corresponds to a specific region that includes part of the 5'- or 3'-flanking region of EE-OM2 In the most preferred case, the EE-24MZ3-specific probe includes or consists (mainly) of (or is complementary to) a sequence having from 8095 to 10095 of identity with the sequence located between nucleotides 1441-1462 of 5EO IO Mo 2, or a sequence that has 80 95 to 100 95 identity with the sequence located between nucleotides 220-260 of IO Mo 3, and the EE-cM2-specific probe includes or consists (primarily) of (or is complementary to) a sequence having 8095 to 10095 identity with the sequence located between nucleotides 301-322 5EO IO Mo 14, or the sequence having from 80 95 to 100 95 of identity

BO with the sequence located between nucleotides 497-518 5EO IO Mo 15.

According to another aspect of the present invention, the described DNA sequences include an event junction site and polynucleotides of sufficient length relating to both the soybean genome and foreign DNA including herbicide resistance genes (transgenes) for each event to enable their use as a primer or probe for the detection of EE-SMZ and

EBE-OMI. Such sequences can include at least 9 nucleotides of soybean genomic DNA and a similar number of nucleotides of foreign DNA, which includes herbicide resistance genes (transgene) EE-3MZ3 or EB-COM'I1, on each side of the connection site, respectively. Most preferably, such DNA sequences include at least 9 nucleotides of soybean genomic DNA and a similar number of nucleotides of foreign DNA comprising herbicide resistance genes 60 (transgene) adjacent to the splice site in 5EO IUO Mo: 2 or ZEO IO Mo: 3, and at least

9 nucleotides of soybean genomic DNA and a similar number of nucleotides of foreign DNA, which includes genes for resistance to herbicides (transgene), adjacent to the connection site in 5EO IU Mo: 12 or

ZEO IO Mo: 13.

According to yet another aspect of the present invention, the described DNA sequences include an event junction site and polynucleotides of sufficient length relating to both the soybean genome and foreign DNA including herbicide resistance genes (transgene) for each event to provide their use as a primer or probe for the detection of EE-SMZ and

EBE-OM2. Such sequences may include at least 9 nucleotides of soybean genomic DNA and a similar number of nucleotides of foreign DNA, including herbicide resistance genes (transgene), for EE-5M3 or EE-SMA2 on each side of the insertion site, respectively. In the most preferred case, such DNA sequences include at least 9 nucleotides of soybean genomic DNA and a similar number of nucleotides of foreign DNA including herbicide resistance genes (transgene) adjacent to the splice site in ZEO IU Mo: 2 or 5EO IO Mo: 3 , and at least 9 nucleotides of soybean genomic DNA and a similar number of nucleotides of foreign DNA, including herbicide resistance genes (transgene), adjacent to the junction site in 5EO IU Mo: 14 or

ZEO OO Mo: 15.

The methods and kits covered by the present invention can be used for various purposes, for example, but not limited to: identification of the presence or determination of the (lower) limit value of EE-OZMZ3 and EE-ZMI or EB-ZMZ3 and EB-CM2 in plants, plant material or products, such as, but not limited to, food or feed (fresh or processed) containing or derived from plant material; additionally or alternatively, the methods and kits of this invention can be used to identify transgenic plant material for the purpose of dividing transgenic and non-transgenic materials; additionally or alternatively, the methods and kits of the present invention can be used to determine the quality (ie, percentage of pure material) of plant material comprising EE-CM3Z and EE-SMI1 or EE-SM3Z and EE-C MI.

The present invention, in addition, relates to the 5'- and/or 3'-flanking regions of EE-3MZ3 OM in combination with specific primers and probes designed on the basis of the 5'- and/or 3-flanking sequences of EE-M1 or EE -CM2, as well as to specific primers and probes,

From those developed on the basis of the 5'- and/or 3'-flanking sequences of EE-OMZ3 in combination with specific primers and probes developed on the basis of the 5- and/or 3'-flanking sequences of EE-СМИ1 or EE-СМ2.

The present invention also relates to genomic DNA obtained from soybean plants containing elite events EE-CM3Z and EE-SMI, or plants containing elite events EE-5M3 and EE-CM2. Such a genomic one

DNA can be used as reference control material in the identification assays described herein.

Also provided herein are plants or cells, parts, seeds, or progeny of a herbicide-resistant transgenic soybean, each comprising at least two elite events, the first of said elite events comprising a foreign DNA comprising: a first chimeric gene comprising a modified gene erzr5 from 2ea tauz5, encoding a glyphosate-resistant ERBR5B enzyme, under the control of a promoter that ensures expression in a plant cell, and i) a second chimeric gene, including a modified Prra gene from Rxheiidotopazh Piogesep5, encoding a herbicide-resistant enzyme -HPRO inhibitors, under the control of a promoter that ensures expression in a plant cell, and the second of these elite events includes a (third) chimeric gene that includes a modified glufosinate resistance gene from 5igeriotusez migidospgotodepev, which encodes the enzyme glufosinate- (or phosphinothricin-) acetyltransferase under the control of the promoter, which ensures expression in the plant cell.

In one embodiment, the first of said elite events includes nucleotides 1 to 1451 of 5EO IO Mo 2 adjacent to said foreign DNA immediately above, and nucleotides 241 to 1408 of 5EBO IJO Mo 3 adjacent to said foreign DNA directly from the bottom, and the second of the specified elite events includes nucleotides 1 to 209 from BEO

IO Mo 12 adjacent to said foreign DNA immediately above and nucleotides 569 to 1000 of 5EO IO Mo 13 adjacent to said foreign DNA immediately below, or the second of said elite events includes nucleotides 1 to 311 of BEO IU Mo 14 adjacent to the indicated foreign DNA immediately above, and nucleotides 508 to 1880 of

ZEO IU Mo 15, adjacent to the indicated foreign DNA immediately below.

In another embodiment, the specified elite event can be obtained by crossing with a soybean plant grown from a reference seed containing the specified events and deposited in 60 ATCC under the accession number PTA-11041 or PTA-11042, or can be obtained from a reference seed containing the first of the specified events and deposited in the МСИМВ under the access number

МСИМВ 41659, as well as from the reference seed containing the second of the specified events and deposited in МСИМВ under accession number МСИМВ 41658 or in МСИМВ under access number

МСИМВ 41660.

In another embodiment, the analysis of the genomic DNA of the indicated soybean plant or its cell, part, seed or offspring using the protocol for the identification of the first of the indicated elite events using two primers that include nucleotide sequences with "EO IU

Mo 4 and 5EO IO Mo 5, respectively, leads to obtaining a DNA fragment with a length of 263 bp. or approximately 263 p.p., and the analysis using the protocol for the identification of the second of the specified elite events using two primers that include the nucleotide sequences of EO 10

Mo 16 and ZEO IO Mo 17, respectively, leads to obtaining a DNA fragment with a length of 183 bp. or approximately 183 p.p., and when using two primers that include the nucleotide sequences of ЗБЕО ИЯ Мо 18 and 5ЕО ИО Мо 19, respectively, leads to the receipt of a fragment

DNA with a length of 151 p.o. or approximately 151 p.o.

In addition, provided herein is a method of identifying a transgenic soybean plant or its cells, parts, seeds, or progeny containing 2 elite events in biological samples in which said plant, cells, seeds, or progeny are resistant to glyphosate, glufosinate, and the HPRO inhibitor herbicide (for example, isoxaflutol), and the specified method includes amplification of a DNA fragment with a length between 100 and 500 bp. from the nucleic acid of the first of the specified events present in the biological sample by polymerase chain reaction using at least two primers, one of which recognizes the 5'-linking region of the first of the above elite events, and said 5'-"rlinking region includes the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1 to nucleotide 1451 or the 3'-flanking region of the first of the specified elite events, and the specified 3-flanking region includes the nucleotide sequence of the complementary chain 5EO IUO Mo C from nucleotide 241 to nucleotide 1408, and the second of the specified of primers recognizes the sequence within the foreign

The DNA of the first of these events, which includes the nucleotide sequence of the complementary chain ZEO IUO Mo 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence

ZBO IO Mo Z from nucleotide 1 to nucleotide 240, and includes amplification of a DNA fragment

With a length between 50 and 1000 p.o. or between 100 and 500 p.o. from the nucleic acid of the second of the specified events present in the biological sample by means of polymerase chain reaction using at least two primers, one of which recognizes the 5'-linking region of the second of the above elite events, and the specified 5'-"rlinking region includes a nucleotide the sequence of ZEO IU Mo 12 from nucleotide 1 to 209 or the 3'-flanking region of the second of the specified elite events, and the specified 3'-flanking region includes the nucleotide sequence of the complementary chain of ZEO IU Mo 13 from nucleotide 569 to 1000, and the second of the specified primers recognizes the sequence within the foreign DNA of the second of the specified events, which includes the nucleotide sequence 5EO IYU Mo 12 from nucleotide 210 to nucleotide 720 or the nucleotide sequence ZEO IYUO Mo 13 from nucleotide 1 to nucleotide 568.

In addition, this document provides a method for identifying a transgenic soybean plant or its cells, parts, seeds or progeny containing 2 elite events in biological samples, in which said plant, cells, seeds or progeny are resistant to glyphosate, glufosinate and/or herbicide-inhibitor HPRO (for example, isoxaflutol), and the specified method includes amplification of a DNA fragment with a length between 50 and 1000 or between 100 and 500 p.o. from the nucleic acid of the first of the specified events present in the biological sample by means of a polymerase chain reaction using at least two primers, one of which recognizes the 5'-flanking region of the first of the above elite events, and the specified

The B'-flanking region includes the nucleotide sequence 5EOO IO Mo 2 from nucleotide 1 to nucleotide 1451 or the 3'-flanking region of the first of the specified elite events, and the specified 3-flanking region includes the nucleotide sequence of the complementary chain 5EO IUO Mo C from nucleotide 241 to nucleotide 1408, and the second of the specified primers recognizes the sequence within the foreign DNA of the first of the specified events, which includes the nucleotide sequence of the complementary chain ZEO IU Mo 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence 5XEO IU Mo C from nucleotide 1 to nucleotide 240, and includes amplification of a DNA fragment with a length between 50 and 1000 bp. or between 100 and 500 p.o. from the nucleic acid of the second of the specified events present in the biological sample by means of polymerase chain reaction using at least two primers, one of which recognizes the 5'-flanking region of the second of the above elite events, and the specified 5'-flanking region includes a nucleotide the sequence of ZEO IU Mo 14 from nucleotide 1 to 311 bo or the 3'-flanking region of the second of the specified elite events, and the specified 3'-flanking region includes the nucleotide sequence of the complementary chain 5EO IO Mo 15 from nucleotide 508 to 1880, and the second of the specified primers recognizes the sequence within the foreign DNA of the second of the specified events, which includes the nucleotide sequence 5EO IU Mo 14 from nucleotide 312 to nucleotide 810 or the nucleotide sequence ZEO IO Mo 15 from nucleotide 1 to nucleotide 507.

Furthermore, provided herein is a kit for identifying a transgenic soybean plant or its cells, parts, seeds or progeny containing 2 elite events and resistant to glyphosate, glufosinate and an HPRO inhibitor herbicide (eg, isoxaflutol) in biological samples, wherein said kit includes a primer that recognizes the 5'-flanking region of the first of the above elite events, and said 5'-flanking region includes the nucleotide sequence

ZEO IO Mo 2 from nucleotide 1 to nucleotide 1451, or a primer that recognizes the 3'-flanking region of the first of the indicated elite events, and the specified 3'-flanking region includes the nucleotide sequence of the complementary chain 5EO IO Mo C from nucleotide 241 to nucleotide 1408, and a primer that recognizes the sequence within the foreign DNA of the first of the specified events, and the specified foreign DNA includes the nucleotide sequence of the complementary chain 5EO IU Mo 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence ZEO IO Mo C from nucleotide 1 to nucleotide 240, and said set includes a primer that recognizes the 5'-flanking region of the second of the above elite events, and said 5'-flanking region includes the nucleotide sequence

ZEO IU Mo 12 from nucleotide 1 to 209, or a primer recognizing the 3'-linking region of the second of the specified elite events, and the specified 3'-linking region includes the nucleotide sequence of the complementary chain of ZEO IU Mo 13 from nucleotide 569 to 1000, and the second of the indicated primers recognizes the sequence within the foreign DNA of the second of the indicated events, which includes the nucleotide sequence 5EO IU Mo 12 from nucleotide 210 to nucleotide 720 or the nucleotide sequence ZEO IUO Mo 13 from nucleotide 1 to nucleotide 568.

Furthermore, provided herein is a kit for identifying a transgenic soybean plant or its cells, parts, seeds or progeny containing 2 elite events and resistant to glyphosate, glufosinate and an HPRO inhibitor herbicide (eg, isoxaflutol) in biological samples, wherein said kit includes a primer that recognizes the 5'-flanking region of the first of the above

From elite events, and the specified 5'-linking section includes a nucleotide sequence

ZEO IO Mo 2 from nucleotide 1 to nucleotide 1451, or a primer that recognizes the 3'-flanking region of the first of the indicated elite events, and the specified 3'-flanking region includes the nucleotide sequence of the complementary chain 5EO IO Mo C from nucleotide 241 to nucleotide 1408, and a primer that recognizes the sequence within the foreign DNA of the first of the specified events, and the specified foreign DNA includes the nucleotide sequence of the complementary chain 5EO IU Mo 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence ZEO IO Mo C from nucleotide 1 to nucleotide 240, and said set includes a primer that recognizes the 5'-flanking region of the second of the above elite events, and said 5'-flanking region includes a nucleotide sequence

ZEO IU Mo 14 from nucleotide 1 to 311, or the 3'-flanking region of the second of the specified elite events, and the specified 3'-flanking region includes the nucleotide sequence of the complementary chain of ZEO IU Mo 15 from nucleotide 508 to 1880, and the second of the specified primers recognizes the sequence within the foreign DNA of the second of the specified events, which includes the nucleotide sequence 5E0 IU Mo 14 from nucleotide 312 to nucleotide 810 or the nucleotide sequence 5EO IU Mo 15 from nucleotide 1 to nucleotide 507.

Also provided herein is a soybean plant, cell, tissue or seed of a plant containing in its genome a nucleic acid molecule comprising a nucleotide sequence at least 97, 98 or at least 99,595 or 99,595 identical to the nucleotide sequence of 5EO IO Mo 20 from position 1452 to position 16638, nucleotide

BO sequences of ХЕО ЮЮ Mo 20 from position 2257 to position 16601 or their complementary chains, or a nucleotide sequence that is at least 97, 98 or at least 99 95 or 99.5 95 identical to ZEO ЙО Мо 20 or its complementary chain containing in its genome, a nucleic acid molecule that includes a nucleotide sequence that is at least 97, 98 or at least 99 95 or 99.5 95 identical to the nucleotide sequence of EE-SMI or EE-

OM2 or their complementary chains, and the reference seed containing EE-CM'I is deposited under accession number МСИМВ 41658, and the reference seed containing EE-СМ2 is deposited under accession number МСИМВ 41660. In one of the embodiments of this invention, the nucleotide the EE-ZMI or EBE-OM2 sequence in said plant, cell, tissue or plant seed is a DNA sequence (e.g., a foreign DNA sequence) in 60 ZEO IO Mo 12 or 13 or a DNA sequence (e.g., a foreign DNA sequence) in ZEO IU

Mo 14 or 15, or a DNA sequence in the plant genome (for example, in deposited seeds containing EE-Z3M1 or EE-CM2 according to the invention), containing ZEO IU Mo 12 and 13 between the first nucleotide of the EC IU Mo 12 sequence the last nucleotide of the sequence

ZEO IO Mo 13, or a DNA sequence in the plant genome containing ZEO IO Mo 14 and 15 between the first nucleotide of the sequence 5EO IU Mo 14 and the last nucleotide of the sequence BEO IU

Mo 15.

In one of the embodiments of this invention, a soybean plant, cell, tissue or seed of a plant containing a nucleic acid molecule in its genome that hybridizes with the nucleotide sequence of ZEO IU Mo 1 or its complementary chain or that hybridizes with the nucleotide sequence of 5EO IU Mo 20 is presented from position 1452 to position 16638 or its complementary chain, or that hybridizes with the nucleotide sequence of 5EO IU Mo 20 or its complementary chain, containing in its genome a nucleic acid molecule that hybridizes with the nucleotide sequence of EE-ZMI1 or EE-OM2 or their complementary chains, and the reference seed containing EE-ZM1 is deposited under the accession number

МСИМВ 41658, and the reference seed containing EE-СМ2 is deposited under accession number МСИМВ 41660. In one of the embodiments of this invention, the nucleotide sequence of EE-MI1 or

EBE-OM2 in the specified plant, cell, tissue or seed of the plant is a foreign DNA sequence in 5EO IU Mo 12 or 13 or a foreign DNA sequence in 5EO IU Mo 14 or 15, or a foreign DNA sequence in the composition of the plant genome (for example, in the deposited seeds containing EE-Z3M1 or EE-CM2 according to the invention), containing ZEO IU Mo 12 and 13 between the first nucleotide of the sequence ES IU Mo 12 and the last nucleotide of the sequence

ZEO IU Mo 13, or a foreign DNA sequence in the plant genome containing ZEO IU Mo 14 and 15 between the first nucleotide of the sequence ZEO IU Mo 14 and the last nucleotide of the sequence zEO IU Mo 15.

A brief description of the drawings

The following Examples, which are not intended to limit the present invention to the specific embodiments described, may be considered in conjunction with the accompanying Figures, incorporated herein by reference, in which:

Fig. 1: Schematic representation of the interaction between the indicated nucleotides

With sequences and primers for the elite event EE-5M3. black rectangle: foreign DNA; shaded rectangle: DNA of plant origin; checkered arrow (a): chimeric gene encoding NRPO RE MUZ6b (see Table 1 for the composition of the chimeric gene); shaded arrow (b): chimeric gene encoding 2TERZRZ5 (for the composition of the chimeric gene, see

Tables 1); black arrows: oligonucleotide primers, figures under the rectangles indicate the position of nucleotides; (c) refers to the complementary chain of the specified nucleotide sequence; Note: the diagram is not to scale.

Fig. 2: Results obtained using the PCR-identification protocol developed for

EE-OM3. Gel loading sequence: Lane 1: Molecular weight marker (ladder 100 p.o.); lanes 2 and 3: DNA samples from soybean plants containing the EE-OM3Z transgenic event; lanes 4-7: DNA samples from transgenic soybean plants that do not contain the elite EE-OM3Z event, but that contain the same herbicide resistance genes (other transformation events); lane 8: wild-type soybean DNA sample; lane 9: no DNA template control; lane 10: molecular weight marker.

Fig. 3: Schematic representation of the interaction between the specified nucleotide sequences and primers for the elite event EE-ZM1. black rectangle: foreign DNA; shaded rectangle: DNA of plant origin; arrow with horizontal hatching (g): chimeric gene encoding phosphinothricin acetyltransferase (for the composition of the chimeric gene, see 5EO IU Mo 11); black arrows: oligonucleotide primers, figures under the rectangles indicate the position of nucleotides; (c) refers to the complementary chain of the indicated nucleotide sequence; Note: the diagram is not to scale.

Fig. 4: Schematic representation of the interaction between the specified nucleotide sequences and primers for the elite event EE-ZM2. black rectangle: foreign DNA; shaded rectangle: DNA of plant origin; arrow with horizontal hatching (g): chimeric gene encoding phosphinothricin acetyltransferase (for the composition of the chimeric gene, see 5EO IU Mo 11); black arrows: oligonucleotide primers, figures under the rectangles indicate the position of nucleotides; (c) refers to the complementary chain of the specified nucleotide sequence; NB: not applicable. Note: the diagram is not to scale.

Fig. 5: PCR-identification protocol developed for EE-SM'I1. Bo gel loading sequence: Lane 1: DNA samples from soybean plants containing the EE-OM'1 transgenic event; track 2:

DNA samples from transgenic soybean plants that do not contain the elite event EE-ZM/1; lane 3: control DNA samples from wild-type soybean plants; lane 4: no DNA template control; lane 5: molecular weight marker.

Fig. 6: PCR identification protocol developed for EE-CM2. Gel loading sequence: Lane 1: DNA samples from soybean plants containing the EE-OM2 transgenic event; lane 2: DNA samples from transgenic soybean plants lacking the EE-ZM2 elite event; lane 3: control DNA samples from wild-type soybean plants; lane 4: no DNA template control; lane 5: molecular weight marker.

Detailed description of the best options for implementing the invention

Incorporation of a recombinant DNA molecule into the plant genome is usually the result of cell or tissue transformation. A specific embedding site is usually due to random embedding.

DNA incorporated into a plant genome by transformation of a plant cell or tissue with recombinant DNA, or "transforming DNA" and derived from such transforming DNA, is hereinafter referred to as "foreign DNA", including one or more "transgenes". Transgenes EE-

CM3Z are genes for resistance to glyphosate and the herbicide-inhibitor NRPO. "Plant DNA" in the context of this invention refers to DNA derived from a transformed plant.

Plant DNA is usually found at the same genetic locus of the corresponding wild-type plant. Foreign DNA can be characterized by its position and configuration in the site of insertion of the recombinant DNA molecule into the plant genome. The site of the plant genome where the recombinant DNA is inserted is also called the "insertion site" or "target site". Insertion of the recombinant DNA into a region of the plant genome, termed 'pre-insertion plant DNA', may be associated with a deletion of the plant DNA, termed a 'target site deletion'. A 'flanking region' or 'flanking sequence' herein refers to a sequence of at least 20 bp, preferably at least 50 bp, and up to 5000 bp of DNA different from the included DNA, preferably plant genome DNA, linked to the foreign DNA directly from above or linked to the foreign DNA directly bottom.The result of transformation procedures that lead to the random incorporation of foreign DNA is the production of transformants with different flanking regions that are characteristic and unique to each

From the transformer. If recombinant DNA is incorporated into a plant's genome by conventional crossing, the site of its insertion in the plant's genome or its flanking regions are generally not altered. "Isolated nucleic acid (sequence)" or "isolated DNA (sequence)" herein refers to a nucleic acid or DNA (sequence) that is no longer in the natural environment from which it was isolated, for example, a nucleotide sequence in the genome of a plant or other host bacteria, or nucleic acid or DNA as a hybrid with DNA or nucleic acid from another source, for example, as a chimeric gene under the control of a promoter that ensures expression in a plant cell.

An event is an (artificial) genetic locus that, as a result of genetic engineering manipulation, carries foreign DNA or a transgene that includes at least one copy of a gene or genes of interest to the researcher. Typical allelic event states are the presence or absence of foreign DNA. The event is described phenotypically by transgene expression. At the genetic level, the event is part of the genetic makeup of the plant. At the molecular level, an event can be described by a restriction map (eg, as determined by Southern blotting) that differs from the upstream or downstream flanking sequences of the transgene, the position of molecular markers, and/or the molecular configuration of the transgene. Typically, DNA transformation that transforms plants that includes at least one gene of interest to the researcher results in a population of transformants containing a collection of individual events, each of which is unique. The event is described by foreign DNA and at least one of the flanking sequences.

An elite event as used herein is an event selected from a group of events obtained by transformation of the same transforming DNA based on the expression and stability of the transgene(s) and its compatibility with the optimal agronomic characteristics of the plant containing the event. Thus, the elite event selection criteria are one or more, preferably two or more, most preferably all criteria from the following list: a. the presence of foreign DNA does not compromise other desirable characteristics of the plant, such as characteristics related to agronomic performance or commercial value; b. the event is described by a well-defined molecular configuration that is stably inherited and for which suitable identity control tools can be developed;

in. the gene(s) of interest to the researcher demonstrate(s) correct, adequate and stable spatial and temporal phenotypic expression in both the heterozygous (or hemizygous) and homozygous states of the event at a commercially acceptable level over a range of environmental conditions, under which normal agronomic use of plants bearing the specified event is possible.

In the best case, the foreign DNA is associated with a position in the genome of the plant, which allows easy incorporation of foreign sequences into a commercially desirable genetic environment.

The elite status of the event is confirmed by the introgression of the elite event into various variants of the genetic environment relevant to the issue under consideration, and by observing compliance with one, two or all of the above criteria, for example, a., 6. and in...

Thus, an "elite event" refers to a genetic locus that contains foreign DNA and meets the criteria described above. A plant, plant material, or progeny, such as a seed, may contain one or more elite events in its genome.

The tools developed to identify an elite event or plant, or plant material containing an elite event, or a product comprising plant material containing an elite event, are based on specific genomic characteristics of the elite event, e.g., a specific restriction map of a region of the genome containing a foreign DNA, molecular markers or the sequence of the linking region(s) of foreign DNA.

After sequencing one or both flanking regions of the foreign DNA, primers and probes can be designed that specifically recognize this sequence(s) in the nucleic acid (DNA or RNA) of the sample using molecular biology techniques. For example, a PCR technique can be developed to identify an elite event in biological samples (eg, plant samples, plant material, or products containing plant material). Such PCR is based on at least two specific "primers", one of which recognizes the sequence within the 5'- or 3'-flanking region of the elite event, and the second recognizes the sequence within the foreign DNA. Preferably, the primers have a sequence of 15 to 35 nucleotides in length that, under PCR-optimized conditions, "specifically recognizes" a sequence within the 5'- or 3'-flanking region of the elite event or foreign DNA of the elite event, respectively, resulting in amplification specific fragment ("integrated fragment" or

From a differential amplicon) from a nucleic acid sample containing an elite event. This means that under conditions optimized for PCR, only the integrated target fragment and no other sequence that is part of the plant genome or foreign DNA is amplified.

PCR primers suitable for the identification of EE-M3 can be: - oligonucleotides with a length from 17 to approximately 200 nucleotides, including

From the 'end, a nucleotide sequence with a length of at least 17 consecutive nucleotides, preferably 20 consecutive nucleotides, which is selected from the DNA of the plant as part of the 5'-flanking sequence (ZEO IO Mo 2 from nucleotide 1 to nucleotide 1451) (primers recognizing 5'-flanking sequences); or - oligonucleotides with a length from 17 to approximately 200 nucleotides, inclusive

From the '-end, a nucleotide sequence with a length of at least 17 consecutive nucleotides, preferably 20 consecutive nucleotides, which is selected from the DNA of the plant as part of the 3'-flanking sequence (the complementary chain of 5EO IUO Mo C from nucleotide 241 to nucleotide 1408) (primers recognizing 3' -flanking sequences); or - oligonucleotides with a length from 17 to approximately 200 nucleotides, inclusive

From the end, a nucleotide sequence with a length of at least 17 consecutive nucleotides, preferably 20 consecutive nucleotides, which is selected from the included DNA sequences (the complementary chain of 5EO IU Mo 2 from nucleotide 1452 to nucleotide 1843) (primers that recognize foreign DNA); or - oligonucleotides with a length of from 17 to approximately 200 nucleotides, including a nucleotide sequence with a length of at least 17 consecutive nucleotides, preferably 20 consecutive nucleotides, which are selected from the included DNA sequences (ZEO IO Mo Z from nucleotide 1 to nucleotide 240); or - suitable oligonucleotides with a length of from 17 to approximately 200 nucleotides, including a nucleotide sequence with a length of at least 17 consecutive nucleotides, preferably 20 consecutive nucleotides, which are selected from the nucleotide sequence of the included DNA fragment or its complementary chain (ZEO IU Mo 1 or 5EO IUO Mo 20 from nucleotide 1452 to nucleotide 16638).

It is clear that the length of the primers can be more than the specified 17 consecutive nucleotides 60 and can be, for example, 20, 21, 30, 35, 50, 75, 100, 150, 200 nucleotides and even more.

Primers can consist entirely of a nucleotide sequence, which is selected from the indicated nucleotide sequences of flanking sequences and foreign DNA sequences. At the same time, the nucleotide sequence of the 5'-end of the primers (i.e., beyond the 17 consecutive nucleotides located at the 3'-end is less critical. Thus, the 5'-sequence of the primers may include or consist of a nucleotide sequence selected from the flanking sequences or of foreign DNA, if necessary, but may contain multiple (e.g., 1, 2, 5, or 10) mismatches. The 5' sequence of the primers may even be entirely a nucleotide sequence unrelated to the flanking sequences or foreign DNA, e.g. sequence representing one or more restriction enzyme recognition sites Such unrelated sequences or flanking DNA sequences with mismatches should preferably not exceed 100 and more preferably 50 or even 25 nucleotides in length.

In addition, suitable primers can contain, consist of, or mainly consist of a nucleotide sequence at their 3'-ends that overlaps the region of the junction of plant DNA sequences and foreign DNA sequences (located at position 1451-1452 in 5EO IYUO Mo 2 and 240-241 in 5BEBEO IU Mo Z for EE-COM3), which makes the specified 17 consecutive nucleotides located at the 3'-end derived from both foreign DNA and sequences of plant origin ZEO IU Mo 2 or 3.

It will be obvious to a person skilled in the art that a correctly selected pair of primers for PCR should not, in addition, contain sequences complementary to each other.

For the purposes of this invention, the "complementary chain of the nucleotide sequence presented in ZEO IO Mo:X" is a nucleotide sequence that is a derivative of the presented nucleotide sequence by replacing nucleotides with their complementary nucleotides in accordance with Chargaff's rules (A - T C) and reading the sequence in direction from 5" to 3", i.e. opposite to the presented nucleotide sequence.

Examples of suitable primers for EE-CM3 are oligonucleotide sequences

ZEO IU Mo 5 (primer recognizing the 3'-flanking sequence), cXEO IU Mo 4 (primer recognizing foreign DNA for use with primers recognizing the 3'-flanking

Zo sequence) or ZEO IJOO Mo 7 (primer recognizing foreign DNA for use with primers recognizing 3'--flanking sequence).

Other examples of suitable oligonucleotide primers for EE-ZMZ contain the following sequences at the 3'-end or consist (mainly) of the following sequences: a. primers recognizing the 5'-flanking sequence: - nucleotide sequence of 5EO IU Mo 2 from nucleotide 264 to nucleotide 283 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 266 to nucleotide 285 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1240 to nucleotide 1259 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 265 to nucleotide 285 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 265 to nucleotide 283 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1239 to nucleotide 1259 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1241 to nucleotide 1259 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1244 to nucleotide 1263 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1248 to nucleotide 1267 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1250 to nucleotide 1269 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 22 to nucleotide 279 - nucleotide sequence 5EO IU Mo 2 from nucleotide 263 to nucleotide 279 - nucleotide to the sequence of 5EO IU Mo 2 from nucleotide 264 to nucleotide 285 - the nucleotide sequence of 5EO IU Mo 2 from nucleotide 266 to nucleotide 283 - the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1238 to nucleotide 1259

BO - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1242 to nucleotide 1259 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1243 to nucleotide 1263 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1245 to nucleotide 1263 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1247 to nucleotide 1267 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1249 to nucleotide 1269 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1249 to nucleotide 1267 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 263 to nucleotide 285 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 267 to nucleotide 283 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1242 to nucleotide 1263 - nucleotide sequence of ZEO IU Mo 2 from nucleotide 1243 to nucleotide 1259 60 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1246 to nucleotide 1267

- nucleotide sequence of 5EO IU Mo 2 from nucleotide 1246 to nucleotide 1263 - nucleotide sequence of ZEO IU Mo 2 from nucleotide 1248 to nucleotide 1269 - nucleotide sequence of 5EO IUO Mo 2 from nucleotide 1250 to nucleotide 1271 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1250 to of nucleotide 1267 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1241 to nucleotide 1263 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1245 to nucleotide 1267 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1247 to nucleotide 1269 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1247 to nucleotide 1263 - nucleotide sequence 5EO IU Mo 2 from nucleotide 1249 to nucleotide 1271 - nucleotide sequence 5EO IU Mo 2 from nucleotide 1242 to nucleotide 1261 - nucleotide sequence 5EO IU Mo 2 from nucleotide 1241 to nucleotide 1261 - nucleotide sequence 25EO IU Mo from nucleotide 1243 to nucleotide 1261 - the nucleotide sequence of 5EO IYUO Mo 2 from nucleo sequence 1240 to nucleotide 1261 - nucleotide sequence of 5EO IO Mo 2 from nucleotide 1244 to nucleotide 1261 - nucleotide sequence of 5EO IU Mo 2 from nucleotide 1239 to nucleotide 1261 - nucleotide sequence of 5EO IO Mo 2 from nucleotide 1245 to nucleotide 1261

BYU primers recognizing foreign DNA for use with primers recognizing the 5'-flanking sequence: - complementary strand of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1732 to nucleotide 1751 - complementary strand of the nucleotide sequence zEO IU Mo 2 from nucleotide 1735 to nucleotide 1754 - complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1731 to nucleotide 1750 - complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1732 to nucleotide 1750 - complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1732 to nucleotide 1752 - complementary chain of the nucleotide sequence zEO IU Mo 2 from nucleotide 1731 to nucleotide 1749 - complementary nucleotide sequence zEO IU Mo 2 from nucleotide 1732 to nucleotide 1749 - complementary chain of nucleotide sequence zEO IU Mo 2 from nucleotide 1731 to nucleotide 1751 - complementary chain of nucleotide sequence zEO IU M o 2 from nucleotide 1732 to nucleotide 1753 - complementary chain of the nucleotide sequence zEO IU Mo 2 from nucleotide 1731 to nucleotide 1748 - complementary chain of the nucleotide sequence zEO IU Mo 2 from nucleotide 1732 to nucleotide 1748 - complementary chain of the nucleotide sequence zEO IU Mo 2 from nucleotide 1735 to nucleotide 1751 - complementary chain of the nucleotide sequence zEO IU Mo 2 from nucleotide 1731 to nucleotide 1752 - complementary chain of the nucleotide sequence zEO IU Mo 2 from nucleotide 1732 to nucleotide 1754 - complementary chain of the nucleotide sequence zEO IU Mo 2 from nucleotide 1731 to nucleotide 1747 - complementary nucleotide sequence zEO IU Mo 2 from nucleotide 1731 to nucleotide 1753 - complementary chain of nucleotide sequence zEO IU Mo 2 from nucleotide 1727 to nucleotide 1746 - complementary chain of nucleotide chain sequence 5EO IU Mo 2 from nucleotide 1727 to nucleotide 1745 - complementary chain nuc of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1727 to nucleotide 1747 - the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1727 to nucleotide 1744 - the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1727 to 60 nucleotide 1748

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1727 to nucleotide 1749

- complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1726 to nucleotide 1745

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1726 to nucleotide 1744

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1726 to nucleotide 1746

- complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1726 to nucleotide 1747

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1726 to nucleotide 1748

- complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1724 to nucleotide 1744

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1724 to nucleotide 1745

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1724 to nucleotide 1746

- complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1461 to nucleotide 1478

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1670 to nucleotide 1686

- complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1469 to nucleotide 1486

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1508 to nucleotide 1527

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1667 to nucleotide 1686

- complementary chain of the nucleotide sequence ZEO IU Mo 2 from nucleotide 1670 to nucleotide 1687

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1673 to nucleotide 1689

- complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1688 to nucleotide 1704

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1688 to nucleotide 1705

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1692 to nucleotide 1709

- complementary chain of the nucleotide sequence ZEO IU Mo 2 from nucleotide 1467 to nucleotide 1486

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1481 to nucleotide 1497

- complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1481 to nucleotide 1498

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1491 to nucleotide 1507

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1491 to nucleotide 1508

- complementary chain of the nucleotide sequence ZEO IU Mo 2 from nucleotide 1672 to nucleotide 1688

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1673 to nucleotide 1690

- complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1673 to nucleotide 1691

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1688 to nucleotide 1706

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1691 to nucleotide 1707

- complementary chain of the nucleotide sequence ZEO IU Mo 2 from nucleotide 1691 to 60 nucleotide 1708

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1469 to nucleotide 1487

- complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1481 to nucleotide 1499

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1489 to nucleotide 1505

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1489 to nucleotide 1506

- complementary chain of the nucleotide sequence ZEO IU Mo 2 from nucleotide 1489 to nucleotide 1507

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1489 to nucleotide 1508

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1666 to nucleotide 1686

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1667 to nucleotide 1687

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1670 to nucleotide 1688

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1672 to nucleotide 1689

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1688 to nucleotide 1707

- complementary chain of the nucleotide sequence 5EO IO Mo 2 from nucleotide 1691 to nucleotide 1709

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1692 to nucleotide 1710

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1481 to nucleotide 1500

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1491 to nucleotide 1509

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1670 to nucleotide 1689

- the complementary chain of the nucleotide sequence ЗЕО ИО Мо 2 from nucleotide 1672 to nucleotide 1690

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1672 to nucleotide 1691

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1687 to nucleotide 1705

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1687 to nucleotide 1706

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1691 to nucleotide 1710

- complementary chain of the nucleotide sequence 5EO IO Mo 2 from nucleotide 1472 to nucleotide 1488

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1488 to nucleotide 1507

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1491 to nucleotide 1510

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1495 to nucleotide 1512

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1495 to nucleotide 1513

- complementary chain of the nucleotide sequence 5EO IO Mo 2 from nucleotide 1495 to nucleotide 1514

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1673 to nucleotide 1692

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1678 to nucleotide 1694

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1678 to 60 nucleotide 1695

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1678 to nucleotide 1696

- complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1687 to nucleotide 1703

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1687 to nucleotide 1704

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1692 to nucleotide 1711

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1469 to nucleotide 1488

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1488 to nucleotide 1506

- complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1491 to nucleotide 1511

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1670 to nucleotide 1690

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1678 to nucleotide 1697

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1688 to nucleotide 1709

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1467 to nucleotide 1487

- complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1488 to nucleotide 1508

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1495 to nucleotide 1511

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1491 to nucleotide 1512

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1666 to nucleotide 1687

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1667 to nucleotide 1688

- complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1672 to nucleotide 1692

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1673 to nucleotide 1693

- complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1687 to nucleotide 1707

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1472 to nucleotide 1490

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1472 to nucleotide 1491

- complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1481 to nucleotide 1501

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1489 to nucleotide 1509

- complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1495 to nucleotide 1515

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1670 to nucleotide 1691

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1673 to nucleotide 1694

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1678 to nucleotide 1698

- the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1469 to nucleotide 1489

- complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1667 to nucleotide 1689

- the complementary chain of the nucleotide sequence of ЭО ИЯ Мо 2 from nucleotide 1687 to 60 of nucleotide 1708

- complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1688 to nucleotide 1710 - complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1691 to nucleotide 1711 - complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1472 to nucleotide 1492 - complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1489 to nucleotide 1510 - complementary nucleotide sequence zEO IU Mo 2 from nucleotide 1666 to nucleotide 1688 - complementary chain of nucleotide sequence zEO IU Mo 2 from nucleotide 1687 to nucleotide 1709 - complementary chain of nucleotide sequence zEO IU Mo 2 from nucleotide 1692 to nucleotide 1712 - the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1467 to nucleotide 1488 - the complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1469 to nucleotide 1490 - the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nuc leotide 1488 to nucleotide 1509 - complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1489 to nucleotide 1511 - complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1678 to nucleotide 1699 - complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1472 to nucleotide 1493 - complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1472 to nucleotide 1494 - complementary chain of the nucleotide sequence zEO IU Mo 2 from nucleotide 1481 to nucleotide 1502 - complementary chain of the nucleotide sequence zEO IU Mo 2 from nucleotide 1670 to nucleotide 1692 - complementary chain of the nucleotide sequence ZEO IU Mo 2 from nucleotide 1469 to nucleotide 1491 - complementary chain of the nucleotide sequence zEO IU Mo 2 from nucleotide 1488 to nucleotide 1510 - complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1691 to nucleotide 1712 - complementary chain of nucleotide pos of the lead of ZEO IU Mo 2 from nucleotide 1692 to nucleotide 1713 - the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1692 to nucleotide 1714 - the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1467 to nucleotide 1489 - the complementary chain of the nucleotide sequence of ZEO IU Mo 2 from nucleotide 1678 to nucleotide 1700 - complementary chain of the nucleotide sequence 5EO IU Mo 2 from nucleotide 1481 to nucleotide 1503 - complementary chain of the nucleotide sequence zEO IU Mo 2 from nucleotide 1691 to nucleotide 1713 p. primers that recognize the 3'-flanking sequence: - the complementary chain of the nucleotide sequence from KHEO IU MoZ from nucleotide 828 to nucleotide 847 - the complementary chain of the nucleotide sequence of KHEO IU MoZ from nucleotide 830 to nucleotide 849 - the complementary chain of the nucleotide sequence of KHEO IU MoZ from from nucleotide 828 to nucleotide 846 - the complementary chain of the nucleotide sequence of ХЕО ЮХ MoЗ from nucleotide 828 to nucleotide 848 60 - the complementary chain of the nucleotide sequence of ХЕО ЮХ MoЗ from nucleotide 830 to nucleotide 848 - the complementary chain of the nucleotide sequence from БЕО IХО MoЗ from nucleotide 830 to nucleotide 850 - complementary chain of the nucleotide sequence of KEO IU Mo Z from nucleotide 828 to nucleotide 845 - complementary chain of the nucleotide sequence of KEO IU Mo Z from nucleotide 830 to nucleotide 847 - complementary chain of the nucleotide sequence of KEO IU Mo Z from nucleotide 828 to nucleotide 849 - complementary chain of nucleotide from nucleotide 830 to nucleotide 851 - the complementary chain of the nucleotide sequence of KEO IU Mo Z from nucleotide 828 to nucleotide 844 - the complementary chain of the nucleotide sequence of KEO IU Mo Z from nucleotide 830 to nucleotide 846 - the complementary chain of the nucleotide sequence of KEO IU Mo C from nucleotide 828 to nucleotide 850 - complementary chain of the nucleotide sequence 5EO IU Mo Z from nucleotide 830 to nucleotide 852 - complementary chain of the nucleotide sequence KEO IU Mo Z from nucleotide 992 to nucleotide 1009 - complementary chain of the nucleotide sequence zBEO IU Mo Z from nucleotide 731 to of nucleotide 752 - complementary chain of the nucleotide sequence of ХЕО ЮЮ Mo З from nucleotide 776 to nucleotide 795 - complementary chain of the nucleotide sequence of ХЕО ЮЮ Mo З from nucleotide 731 to nucleotide 753 - complementary chain of the nucleotide sequence 5EO ЮХ Mo З from nucleotide 776 to nucleotide 794 - complementary of the nucleotide sequence of HEO IU Mo Z from nucleotide 776 to nucleotide 796 - complementary chain of the nucleotide sequence of HEO IU Mo Z from nucleotide 776 to nucleotide 793 - complementary chain of the nucleotide sequence of HEO IU Mo C chain from nucleotide 776 to nucleotide 797 - complementary chain of the nucleotide sequence of HEO IU Mo C from nucleotide 776 to nucleotide 792 - complementary chain of the nucleotide sequence 5E0 IUO Mo C from nucleotide 776 to nucleotide 798 - complementary chain of the nucleotide sequence KHEO IU Mo C from nucleotide 733 to nucleotide 752 - complementary chain of the nucleotide sequence KHEO IU Mo C from nucleotide 733 to nucleotide 753 - the complementary chain of the nucleotide sequence of ХЕО ЮЮ Mo З from nucleotide 733 to nucleotide 754 - the complementary chain of the nucleotide sequence ХЕО ЮЮ Mo З from nucleotide 733 to nucleotide 755 - the complementary chain of the nucleotide sequence 5EO ЮЮ Mo З from nucleotide 838 to nucleotide 854 - com complementary chain of the nucleotide sequence of ХЕО ЮЮ Mo З from nucleotide 246 to nucleotide 263 - complementary chain of the nucleotide sequence of ХEO ЮЮ Mo З from nucleotide 838 to nucleotide 855 - complementary chain of the nucleotide sequence of ХЕО ЮЮ Mo З from nucleotide 245 to nucleotide 264 a.

Primers recognizing foreign DNA for use with primers recognizing the 3'-flanking sequence: - nucleotide sequence 5EO IU MoZ from nucleotide 173 to nucleotide 192 - nucleotide sequence 5EO IUO MoZ from nucleotide 22 to nucleotide 41 60 - nucleotide sequence 5EO IU MoZ from nucleotide 172 to nucleotide 192

- nucleotide sequence of 5EO IU MoZ from nucleotide 174 to nucleotide 192 - nucleotide sequence of 5EO IU MoZ from nucleotide 191 to nucleotide 210 - nucleotide sequence of 5EO IU MoZ from nucleotide 171 to nucleotide 192 - nucleotide sequence of 5EO IU MoZ from nucleotide 175 to of nucleotide 192 - nucleotide sequence of 5EO IU MoZ from nucleotide 190 to nucleotide 210 - nucleotide sequence of 5EO IU MoZ from nucleotide 192 to nucleotide 210 - nucleotide sequence of 5EO IU MoZ from nucleotide 176 to nucleotide 192 - nucleotide sequence of 5EO IU MoZ from nucleotide 189 to nucleotide 210 - nucleotide sequence of 5EO IU MoZ from nucleotide 193 to nucleotide 210 - nucleotide sequence of 5EO IU MoZ from nucleotide 188 to nucleotide 210 - nucleotide sequence of 5EO IU MoZ from nucleotide 194 to nucleotide 210 - nucleotide sequence of 5EO IU MoZ from nucleotide 199 to nucleotide 218 - nucleotide sequence of 5EO IU MoZ from nucleotide 200 to nucleotide 218 - nucleotide sequence of 5EO IU MoZ from nucleotide 197 to nucleotide 218 - nucleotide sequence of 5EO IU MoZ from nucleotide 201 to nucleotide 218 - nucleotide sequence of 5EO IU MoZ from nucleotide 201 to nucleotide 220 - nucleotide sequence of 5EO IU MoZ from nucleotide 200 to of nucleotide 220 - nucleotide sequence of 5EO IU Mo Z from nucleotide 199 to nucleotide 220 - nucleotide sequence of 5EO IU Mo Z from nucleotide 200 to nucleotide 221 - nucleotide sequence of 5EO IUO Mo Z from nucleotide 199 to nucleotide 221 - nucleotide sequence of 5EO IU Mo Z from nucleotide 150 to nucleotide 172

PCR primers suitable for the identification of EE-media are described in application M/02006/108674, in particular, from page 8 line 4 to page 26 line 7 (incorporated herein by reference).

PCR primers suitable for the identification of EE-ZM2 are described in application M/O2006/108675, in particular, from page 8 line 4 to page 33 line 4 (incorporated herein by reference).

In this description, "nucleotide sequence of 560 IU Mo 2 from position X to position Y" means a nucleotide sequence including both terminal nucleotides.

Preferably, the amplified fragment is between 50 and 500 nucleotides in length, for example between 100 and 350 nucleotides. The specific primers can have a sequence 80-10095 identical to the sequence within the 5- or 3-flanking region of the elite event or foreign DNA of the elite event, respectively, provided that the mismatches allow specific identification of the elite event when using these primers under conditions optimized for PCR. At the same time, the range of acceptable mismatches is easy to determine experimentally, and these ranges are known to those skilled in the art.

Detection of integrated fragments can be carried out in various ways, for example, by estimating the size of fragments after analysis in a gel. Integrated fragments can also be directly sequenced. In addition, other methods of detecting amplified DNA fragments specific to sequences are known.

Since the sequence of primers and their relative location in the genome are unique to the elite event, amplification of the integrated fragment occurs only in biological samples containing the nucleic acid of the elite event. In the best case, when performing PCR to identify the presence of elite events EE-ZM3Z and EE-SMI or elite events EE-OM3Z and EE-OM2 in unknown samples, include a control with a set of primers, when using which it is possible to amplify a fragment within the "housekeeping gene" plant species containing the specified event. Housekeeping genes are genes expressed in most cell types and associated with metabolic activity common to all cells. In the best case, the size of the fragment amplified from the housekeeping gene exceeds the size of the amplified integrated fragment. Depending on the samples analyzed, other controls may be included.

Standard PCR protocols are described, for example, in "RSK Arriisayop5 Mapiai!" (Cospe Moiesshag

Viospetisa!5, 2pa Eaiiop, 1999) and other sources. Optimal conditions for PCR, including the sequence of specific primers, are specified in the "Protocol for PCR Identification (or Identification by Polymerase Chain Reaction)" for each elite event. At the same time, it is necessary to understand that there may be a need to correct a number of protocol parameters

PCR-identification to laboratory-specific conditions and their slight modification to obtain similar results. For example, when using different methods of preparation

DNA may require correction, for example, the number of primers used, polymerase and hybridization conditions. Similarly, the choice of other primers may dictate other optimal conditions of the PCR-identification protocol. However, these correction options are obvious to specialists and, moreover, are described in detail in modern guidelines for the use of PCR, for example, in the above-mentioned guide.

As an alternative for the amplification of integrated Fragments, which can be used as "specific probes" for the identification of EE-CMZ3 and EE-CMI or EE-CM3Z and EE-CM2 in biological samples, you can use specific primers. The interaction of the nucleic acid of a biological sample with probes under conditions in which hybridization of the probe with the corresponding fragments of the nucleic acid of the sample is possible leads to the formation of nucleic acid/probe hybrids. The formation of these hybrids can be detected (e.g., by nucleic acid or probe labeling), whereby the formation of these hybrids indicates the presence of

EE-OMZ3 and EB-SMI or EB-OMZ3 and EB-SM2. Such identification methods, which are based on hybridization with a specific probe (on a solid-phase carrier or in solution), are described in special literature. In the best case, the specific probe is a sequence that, under optimized conditions, specifically hybridizes with a site within the 5'- or 3-flanking site of an elite event, and also preferably contains a fragment of foreign DNA continuous with this site (hereinafter referred to as "specific site "). In the best case, the specific probe includes a sequence with a length of from 50 to 500 bp, preferably from 100 to 350 bp, which is at least 8095, preferably 80-8595, more preferably 85-90 95, especially preferably 90 -95 95, most preferably by 95 95-100 95, identical (or complementary) to the nucleotide sequence of a specific site. In the best case, the specific probe includes a sequence of about 15 to about 100 contiguous nucleotides identical to (or complementary to) the specific site of the elite event.

In addition, using the specific information presented in this document about the sequence of an elite event, it is possible to develop specific methods for detecting elite events of EE-

CM3Z and EE-SOSMI or elite events EE-CM3Z and EE-CM2, which differ from PCR-based amplification methods. Such alternative detection methods include linear signal amplification detection methods based on invasive cleavage of specific nucleotide structures, also known as IpmaadaegTM technology (as described, for example, in US patents 5985557 "Ipmazime Sieahade oi Mysieis Asidv", 6001567 "Oyeiesiup oi Mysivieis Asii zedoepsez Bu Ipuladeg

In order to detect EE-CM3Z using this method, the target sequence can be hybridized with a labeled first oligonucleotide that includes the nucleotide sequence ZEO IO Mo 2 from nucleotide 1452 to nucleotide 1469 or its complementary chain , or the indicated nucleotide probe, which includes the nucleotide sequence of ЗЕО ЙУ MoС from nucleotide 223 to nucleotide 240 or its complementary chain, and then hybridize with the second oligonucleotide, which includes the nucleotide sequence of 5EO ИО Mo 2 from nucleotide 1434 to nucleotide 1451 or its complementary chain , or the indicated nucleotide probe, which includes the nucleotide sequence 5EO IO MoZ from nucleotide 241 to nucleotide 258 or its complementary chain, and the first and second oligonucleotide overlap by at least one nucleotide. The double or triple structure that will be formed during this hybridization makes it possible to selectively cleavage of the probe with an enzyme (Sieama zeFf) while preserving the target sequence.

After that, detection of the cleaved labeled probe is carried out, possibly through an intermediate step, which leads to further amplification of the signal. To detect EE-ZM1 using this method, the target sequence can be hybridized with a labeled first oligonucleotide, which includes the nucleotide sequence of ZEO ІО Mo 12 from nucleotide 210 to nucleotide 227 or its complementary chain, or with the indicated nucleotide probe, which includes the nucleotide sequence 5EO ІЮ Mo 13 from nucleotide 561 to nucleotide 568 or its complementary chain, and then hybridize with the second oligonucleotide, which includes the nucleotide sequence of 5EO IU Mo 12 from nucleotide 192 to nucleotide 209 or its complementary chain, or with the specified nucleotide probe, which includes the nucleotide sequence of ЧЕО 10

Mo 13 from nucleotide 569 to nucleotide 586 or its complementary chain, and the first and second oligonucleotide overlap by at least one nucleotide. The double or triple structure formed during this hybridization enables selective cleavage of the probe by an enzyme (Sivamazetf) while preserving the target sequence. After that, detection of the cleaved labeled probe is carried out, possibly through an intermediate step, which leads to further amplification of the signal. To detect EE-ZM2 using this method, the target sequence can be hybridized with a labeled first oligonucleotide, which includes the nucleotide sequence 5EO I Mo 14 from nucleotide 312 to nucleotide 329 or its complementary chain, or with the specified nucleotide probe, which includes the nucleotide sequence 5EO IU 60 Mo 15 from nucleotide 490 to nucleotide 507 or its complementary chain, and then hybridize with the second oligonucleotide, including the nucleotide sequence 5EO IU Mo 14 from nucleotide 294 to nucleotide 311 or its complementary chain, or with the indicated nucleotide probe, including the nucleotide sequence ZEO IU

Mo 15 from nucleotide 508 to nucleotide 525 or its complementary chain, and the first and second oligonucleotide overlap by at least one nucleotide. The double or triple structure formed during this hybridization enables selective cleavage of the probe by an enzyme (Sivamazetf) while preserving the target sequence. After that, detection of the cleaved labeled probe is carried out, possibly through an intermediate step, which leads to further amplification of the signal.

The term "kit", as used here, refers to a set of reagents for carrying out the method according to the invention, in particular, the identification of the elite event EE-ZM3 in biological samples or the determination of the viability status of plant material containing EE-CM3. In particular, the best embodiment of the set, according to the invention, includes at least one or two specific primers, according to the above description, for the identification of an elite event, or three specific primers for determining the readiness status. Optionally, the kit may also include any other reagent described herein in the PCR-identification protocol. Alternatively, according to another embodiment of the present invention, the kit may include a specific probe, according to the above description, that specifically hybridizes with the nucleic acid of biological samples to identify the presence of EE-OM3 in them.

Optionally, the kit may also include any other reagent (including buffer for hybridization, label, but not limited to them) for identification of EE-CM3Z in biological samples using a specific probe.

The kit according to the invention can be used and its components can be specifically adapted for the purposes of quality control (eg purity of seed lots), detection of the presence or absence of an elite event in plant material, including plant material or its derivatives that exist, for example, but not limited to, in food or feed.

As used herein, "sequence identity" with respect to nucleotide sequences (DNA or RNA) refers to the number of positions containing identical nucleotides divided by the number of nucleotides in the shorter of the two sequences.

Alignment of two nucleotide sequences is carried out according to the algorithm of Wilber and Lippmann (Umirig apa Sirtapp, 1983, Rhos. Mai. Asad. 5si. OBA 80:726), using a window size equal to 20 nucleotides, a word length equal to 4 nucleotides, and a penalty per skip, equal to 4. Computer analysis and interpretation of sequence data, including sequence alignment as described above, can be performed, for example, using the Sepeis5 Sotrshieg sSgoir sequence analysis software package (s.o.o., University of Wisconsin Biotechnology Center). Sequences are said to be "substantially similar" if such sequences have an identity of at least about 7595, in particular, at least about 80 95, more specifically at least about 85 95, even more specifically at least about 90 95, especially at least about 95 965, the greatest at least about 9895 or at least about 9995.

Obviously, if RNA sequences are said to be substantially similar to or have some degree of identity with DNA sequences, then thymidine (T) in the DNA sequence is equated to uracil ()) in the RNA sequence. Furthermore, it is apparent that small mismatches or mutations may subsequently occur in the DNA sequences and that some mismatches may be acceptable for event-specific primers or probes according to the invention, so any DNA sequence specified herein in any embodiment of this of the invention for any 3'- or 5'-flanking DNA, or for any incorporated or foreign DNA, or any primer or probe according to the invention, also includes sequences substantially similar to those presented herein, e.g. , sequences that hybridize to or have at least 90 95 , 9595 , 96 95 , 97 95 , 9895 , or at least 99 95 identity to sequences given for any 3- or 5'-flanking DNA, for any primer or probe , or for any included or foreign DNA according to the present invention.

The term "primer" as used herein includes any nucleic acid capable of priming the synthesis of the resulting nucleic acid in a matrix synthesis process, such as PCR. Usually, primers are oligonucleotides with a length of 10 to 30 nucleotides, however, longer sequences can be used. Primers can be in double-stranded form, although single-stranded form is preferred. Probes can be used as primers, however, they are designed to bind to DNA or RNA

targets and are not needed in the amplification process.

The term "recognize", as used herein in reference to specific primers, refers to the fact that the specific primers specifically hybridize to the DNA sequence of the elite event under the conditions established according to the method (eg, under the conditions according to the PCR-identification protocol), the specificity being determined using positive and negative controls.

The term "hybridizing", as used herein with respect to specific probes, refers to the fact that the probe binds to a specific region of the nucleotide sequence of the elite event under conditions of standard stringency. Standard stringency conditions, as used herein, refer to the hybridization conditions described herein or to the standard hybridization conditions as described in ZatrbookKei ai., 1989 (MoiIesshiag SiIopipd: A Garogaigu Mapiai, Zesopa Ediiop, Soia zrgipd Nagrog Igarogaigu Rge55, MU) , which, for example, may include the following steps: 1) immobilization of plant genomic DNA fragments on the filter, 2) preliminary hybridization of the filter for 1-2 hours at 42 "С in 50 95 formamide, 5 X standard phosphate-salt buffer with EDTA (З5РЕ ), 2 X Denhardt's reagent and 0.1 95 SDS or within 1-2 hours of approx. 8 "Sv b x standard citrate-salt buffer (550), 2 X Denhardt's reagent and 0.195 SDS, 3) introducing a labeled hybridization probe, 4) incubation for 16-24 hours, 5) washing the filter for 20 minutes. at room temperature in 1 X 55C, 0.195 DSN, 6) washing the filter three times for 20 min., each time at 68"C in 02 X 550, 0.195 DSN, and 7) exposing the filter for 24-48 hours to X-ray film at - 70 "C with a reinforcing screen.

As used herein, a biological sample is a sample of a plant, plant material, or products containing plant material. The term "plant" includes the tissues of a cultivated soybean plant (Siusipe tah) at any stage of development, and any cells, tissues or organs collected or derived from such a plant, including any seeds, leaves, stems, flowers , roots, single cells, gametes, cell cultures, tissue cultures or protoplasts, but not limited to them. "Plant material" as used herein refers to material obtained from or derived from a plant. Products incorporating plant material refer to food, feed or other products,

From those made using plant material, or possibly contaminated plant material. It should be understood that in the context of this invention, such biological samples are tested for the presence of nucleic acids specific for EE-29M3Z3, EE-ZMI1 and EE-CM2, assuming the presence of nucleic acids in these samples. Thus, the methods in this application for the identification of the elite event EE-20MZ3 and EE-ZM2 or EB-ZM3 and EBE-OM'I1 in biological samples refer to the identification of nucleic acids that include the specified elite event in biological samples.

As used herein, the term "comprising" shall be understood to mean the presence of the specified properties, units, steps, reagents or components to which the term refers, without excluding the presence or addition of one or more properties, units, steps, components or groups thereof. So, for example, a nucleic acid or protein comprising a nucleotide or amino acid sequence may include, in addition to those actually specified, additional nucleotides or amino acids, that is, be part of a larger nucleic acid or protein. A chimeric gene comprising a functionally or structurally defined DNA sequence may include additional DNA sequences, for example, promoter and transcription terminator sequences.

The present invention also relates to the development of a combination of an elite event EE-M3Z and an elite event

EBE-OMI1 or combinations of the elite event EE-CM3Z and the elite event EE-CM2 in soybean, to plants comprising the combination of these events, to progeny derived from these plants, and to plant cells or plant material derived from plants that include the specified combinations.

Plants including the elite events EE-ZM3 and EE-M1 or EE-COM3Z and EE-OM2 can be obtained according to the description in example 1. Combinations are obtained by crossing plants containing individual events using generally accepted methods of crossing and identifying them offspring containing two different events.

Soybean plants or plant material containing EE-CM3Z and EE-CM'I can be identified according to the PCR identification protocol described for EE-OMZ and EE-OMI1 in Example 2.

Abbreviated soybean genomic DNA present in the biological sample is subjected to PCR amplification using a primer that specifically recognizes a sequence within the 5'- or 3'-flanking sequence of EE-M3, for example, a primer with the sequence ZEO IU Mo: 5, and a primer that recognizes the sequence of foreign DNA, for example, a primer with the sequence 5EO IO Mo: 4, or also using a primer that specifically recognizes the sequence within the 5'- or 3-

of the flanking sequence of EE-MMI1, for example, a primer with the sequence 5EO IO Mo: 16, and a primer that recognizes the sequence of foreign DNA, for example, a primer with the sequence 5EO

IO Mo: 17.

Soybean plants or plant material containing EE-CM3Z and EE-CM2 can be identified according to the PCR identification protocol described for EE-OMZ and EE-OM2 in Example 2.

Abbreviated soybean genomic DNA present in the biological sample is subjected to PCR amplification using a primer that specifically recognizes a sequence within the 5'- or 3'-flanking sequence of EE-M3, for example, a primer with the sequence ZEO IU Mo: 5, and a primer that recognizes the sequence of foreign DNA, for example, a primer with the sequence 5EO IO Mo: 4, and also using a primer that specifically recognizes a sequence within the 5'- or 3-flanking sequence of EE-ZM2, for example, a primer with the sequence 5EO IO Mo : 18, and a primer that recognizes the sequence of foreign DNA, for example, a primer with the sequence of BEO

IO Mo: 19.

DNA primers that amplify a part of the endogenous soybean sequence are used as a positive control for PCR amplification. If PCR amplification of the material yields fragments of the expected size, the material contains plant material from a soybean plant carrying the elite event EE-CM3Z and EE-OMI, or from a soybean plant carrying the elite event EE-CM3Z and EE-CM2.

Plants carrying EE-CM3Z and EE-CMI1 or EB-CM3Z and EE-CMa2 are characterized by resistance to glyphosate, as well as to HPRO inhibitors, for example, isoxaflutol, as well as resistance to glufosinate. Plants carrying combinations of events, in the absence of herbicide application, also have agronomic characteristics comparable to commercially available soybean varieties. The presence of foreign DNA in the insertion regions of the soybean plant genome described here has been found to confer particularly interesting phenotypic and molecular characteristics to plants containing this event.

One of the variants of the implementation of this invention is a combination of elite events EE-CM3Z and

EBE-OMI1 and/or EE-2M2 in soybean plants, which can be obtained by inserting transgenes at specific positions in the soybean genome, and these elite events confer resistance in such soybean plants to glyphosate, glufosinate, and HPRO inhibitor herbicides such as isoxaflutol, and do not affect the agronomic productivity of such soybeans, negatively affecting the yield

Of such soybean plants compared to isogenic lines (as used herein, "isogenic lines" or "near-isogenic lines" are soybean lines that have the same genetic environment but do not contain transgenes, e.g., plants with a genetic environment similar to the environment of the plant used for transformation or segregating sister lines that have lost the transgenes). In particular, the present invention represents a combination of elite events EE-SMZ and EE-OMI and/or EE-OM2 in soybean plants, and the insertion or presence of the specified elite event in the genome of such soybean plants does not cause increased susceptibility to diseases, loss of yield or increased lodging of such soybean plants in comparison with isogenic lines. Therefore, the present invention represents a combination of elite events in soybean plants, which are designated as EE-CM3Z and EE-OMI1 and/or EE-CM2, which leads to the production of soybean plants resistant to the use of glyphosate, glufosinate and HPRO inhibitor herbicides (simultaneously or separately), and has no adverse effect on said soybean plants compared to isogenic lines, and said soybean plants have no statistical differences in disease susceptibility or dormancy compared to isogenic soybean plants. These characteristics make this combination of elite events very interesting for the control of glyphosate-resistant weeds in soybean fields, and can also be used in approaches to prevent or delay the further development of glyphosate resistance in soybean fields (for example, by applying glyphosate and isoxaflutol, and /or glufosinate, or isoxaflutol and glyphosate, and/or glufosinate, or glufosinate and glyphosate, and/or isoxaflutol, ensuring the use of herbicides with at least 2 or even three different mechanisms of action).

Also presented herein are soybean plant or xx fragments containing the EE-OM3Z event and the elite EE-Z2MI1 or EE-CM2 event, and soybean seed samples containing the EE-CM3 event deposited in

МСИМВ under accession number 41659, soybean seed samples containing event EE-OM/1 deposited in

МСИМВ under accession number МСИМВ 41658, soybean seed samples containing event EE-СМ2 deposited in МСИМВ under accession number МСИМВ 41660, soybean seed samples containing event

EBE-OM3Z and EE-SMI deposited in ATCC under accession number PTA-11041, and soybean seed samples containing event EE-ZM3Z and EE-CM2 deposited in ATCC under accession number PTA-11042.

Soybean plants or fragments thereof containing EE-2MZ and EE-SMI1 or EE-CM3Z and EE-CM2 can be obtained by combining the corresponding elite events found in the respective deposited seed by any means, known in the area, including the crossing of plants obtained from deposited seeds, the collection of their progeny, and the identification among these progeny of plants containing the appropriate combination of elite events. In addition, seeds of such plants containing such events, as well as soy products made from such seeds containing the EE-CMZ3 and EE-CMI or EE-CM2 events, are presented here. Such soy products can be or contain groats, ground seeds, flour, flakes, etc. In particular, such soy product contains nucleic acid that produces amplicons specific for the event EE-MZ3 and the elite event EE-2M1 or EE-COM2, which include 5EO IU Mo 2 or 3, 5EO IU Mo 14 or 15, and/or 5EO IO Mo 12 or 13. In addition, a method for producing a soybean product is provided herein, which includes producing soybean seeds containing the EE-CM3Z event and the elite event EE-SMI or EE-

OMA, and the production of such a soy product from them.

In addition, a soybean plant is presented here that is a progeny of each of the above-described soybean plants containing the EE-CM3Z and EE-OMI1 or EE-CM2 events.

In addition, a method for producing a soybean plant resistant to the herbicides glyphosate and/or glufosinate and/or isoxaflutol is provided herein, comprising incorporating into the genome of such a plant

EE-CM3 with EE-CMI or EE-CM2 events, in particular, by crossing the first soybean plant containing the EE-CM3 event with a soybean plant containing EE-CMI1 and/or EE-CM2 and by selecting progeny resistant to glyphosate and/or glufosinate, and/or isoxaflutol.

In addition, a plant resistant to glyphosate and glufosinate and/or isoxaflutol without loss of yield is presented here, having acceptable agronomic characteristics, containing 2t1ERBRB, NRPO, and the RAT protein, and capable of producing an amplicon specific for EE- events

SM and EE-SOMI1 or EE-SM3Z and EE-SM2.

In addition, a method for weed control in a soybean field containing events EE-5M3Z and EE-SMI or EE-CM3Z and EE-CM2, or in a field intended for planting such soybean plants, including field treatment with an effective by the amount of herbicide based on isoxaflutol, glyphosate and/or glufosinate, and such plants have resistance to such herbicide(s).

In addition, provided herein is a soybean plant, tissue or seed containing EE-OM3Z and EE-SM'I characterized by the presence in the genome of a nucleotide sequence having at least 80 95, 90 Fo, 95 95 or 100 95 identity with EO IU Mo 2 from nucleotide 1431 to nucleotide 1472, and a nucleotide sequence having at least 80 95, 90 95, 95 95 or 100 95 identity with

ZEO IUO Mo 3 from nucleotide 220 to nucleotide 261, or complementary chains of such sequences, as well as a nucleotide sequence having at least 80 95, 90 Fo, 95 95 or 100 95 identity with ZEO IO Mo 12 from nucleotide 199 to nucleotide 220, and nucleotide sequence having at least 80 95, 90 9", 95 95 or 100 95 identity with zEO IU Mo 13 from nucleotide 558 to nucleotide 579, or complementary chains of such sequences.

In addition, a soybean plant, tissue or seed containing EE-OM3Z and EE-CM2, characterized by the presence in the genome of a nucleotide sequence having at least 80 95, 90 Uo, 95 95 or 100 95 identity with EO IU Mo 2 from nucleotide 1431 to nucleotide 1472, and a nucleotide sequence having at least 80 95, 90 95, 95 95 or 100 95 identity with

ZEO IUO Mo 3 from nucleotide 220 to nucleotide 261, or complementary chains of such sequences, as well as a nucleotide sequence having at least 80 95, 90 Fo, 95 95 or 100 95 identity with ZEO IO Mo 14 from nucleotide 301 to nucleotide 322, and nucleotide sequence having at least 80 95, 90 9", 95 95 or 100 95 identity with zEO IU Mo 15 from nucleotide 497 to nucleotide 518, or complementary chains of such sequences.

As used herein, the term "isoxaflutol" refers to the herbicide isoxaflutol i.e. (o-cyclopropyl-4-isoxazolyl)|2-(methylsulfonyl)-4--trifluoromethyl)phenyl|methanone), its active metabolite, diketonitrile, and any mixture of solutions , containing the specified compounds. HPPO-inhibiting herbicides suitable for treating plants containing events according to the present invention are diketonitrile, for example, 2-cyano-3-cyclopropyl-1-(2-methylsulfonyl-4-trifluoromethylphenyl)propane-1,3- dione and 2-cyano-1-(4-(methylsulfonyl)-2-trifluoromethylphenyl|-3-(1-methylcyclopropyl)propane-1,3-dione; other isoxazoles and pyrazolinates, e.g. topramesone, i.e. (3-( 4,5-dihydro-3-isoxazolyl)-2-methyl-4-(methylsulfonyl)phenyl(5-hydroxy-1-methyl-1H-pyrazol-4-yl)methanone and pyrasulphotol ((5-hydroxy-1 .

In one embodiment of the present invention, a field intended for planting soybeans containing an EBE-ZM3 event can be treated with an NRPO inhibitor herbicide, for example, isoxaflutol (IT), before planting soybeans or sowing seeds, which clears the field of weeds plants destroyed by the HPRO inhibitor, and makes it possible to do zero tillage with planting or sowing of soybeans on this pre-treated field at a later time (the application of the HPRO inhibitor herbicide is burned off). Residual activity (ET also protects soybean seedlings and growing plants from early weed competition. After soybeans reach a certain size and weeds begin to re-emerge, glyphosate or an HPRO-glyphosate inhibitor mixture can be applied as a post-emergence herbicide over tops of plants.

In yet another embodiment of the present invention, the field on which the seeds containing the EE-CM3 event are sown can be treated with an NRPO inhibitor herbicide, for example, IRT, before the emergence of soybean plants, but after sowing the seeds (the field can be cleared of weeds plants to seed by other means, of course using conventional tillage practices such as ploughing, tillage or seedbed preparation), and the residual activity will keep the field free of weeds that are killed by the herbicide, ensuring that there is no competition for seedlings and growing plants on the part of weedy plants (pre-emergence application of herbicide-inhibitor of NRPO). After soybean plants reach a certain size and weedy plants begin to re-emerge, glyphosate or an HPRO inhibitor-glyphosate mixture can be applied as a post-emergence herbicide over the tops of the plants.

In another embodiment of the present invention, plants containing the EE-ZM3Z event can be treated with an HPRO inhibitor herbicide, for example, IET, over the tops of soybean plants (emerged from the sown seed), which clears the field of weeds that are killed by the HPRO inhibitor , and this application can be made together with (for example, as a result of mixing in a sprayer), before or after treatment with glyphosate as a post-emergence herbicide over the tops of plants (post-emergence application of an HPRO inhibitor herbicide (with or without glyphosate)).

In addition, according to the present invention, soybean plants carrying EE-OM3Z and EE-OMI1 or EE-

CM2, can be treated with the following insecticides, herbicides or fungicides, or soybean seeds carrying EE-CM3 and EE-3M1 or EE-OM2 can be coated by coating with a coating containing the following insecticides, herbicides or fungicides:

Soybean herbicides: alachlor, bentazone, trifluoroalin, chlorimuron-ethyl, chloransulam-methyl, fenoxaprop, fomesafen, fluazifop, glyphosate, imazamox, imazaquin, imazethapyr, (5-) metolachlor, metribuzin, pendimethalin, tepraloxidim, isoxaflutol, glufosinate.

Soybean insecticides: lambda-cyhalothrin, methomyl, parathion, thiocarb, imidacloprid, clothianidin, thiamethoxan, thiacloprid, acetamiprid, dinotefuran, flubendiamide, rinaxipyr, cyazipyr, spinosad, spinotaram, emamectin benzoate, fipronil, ethiprole, deltamethrin, B-cyfluthrin, gamma - and lambda-cyhalothrin, 4-(I(6b-chloropyridin-3-yl)methyl|(2,2-difluoroethyl)amino|furan-2(5H)-one, spirotetramat, spirodiclofen, triflumuron, flonikamid, thiodicarb, beta -dcifluthrin.

Fungicides for soybeans: azoxystrobin, cyproconazole, epoxyconazole, flutriafol, pyraclostrobin, tebuconazole, trifloxystrobin, prothioconazole, tetraconazole.

The following examples describe the identification of the elite events EE-SMZ3, EE-MSI and EB-OMAa, and plants containing the combination of the event EE-5M3 with EE-ZMI or EE-SOMZ with EE-CM2, and the development of tools for specific identification of the elite event EE-YAMZ3, EE-OZMI or EE-OMA and their combinations in biological samples.

Unless otherwise noted in the Examples, all recombinant techniques were performed in accordance with standard protocols as described in "Zatbgook U apa KizhzeiY OUU (Edv.) (2001) Moyesshiag Siopipd: A I abogaiyutu Mapiai, Za Edyop, Soid brgipd Natog I arogaigu

Rheb55, Mem/ Mogk" and in "Khyzyreia! HA, Vgepi K, Kipobiop KE, Mooge BO, Zeiytap Us, Ztiyp UA apa

ZigypI TO (eaz.) (2006) Sitggepi Rgoiosoi5 ip MoiIesshiag Vioiodu. duopp Umieu 5 Bopv, Mem Mogk".

Standard materials and references are described in "Sgtou KOO (ea.) (1993) Riapi MoiIesciag Vioiodu

Gargah, VIO5 Zsiepiyys Rybiiepege Tsa., Okhtoga apa ViasKueiR! (1998) MoiIesshag Viiodu GargRah, 2pa Edyop, Asadetis Rgez5, Zap Oiedo". Standard materials and techniques for polymerase chain reactions (PCR) are contained in "MeRPeghop MU apa

Meieg 5O (2000) RSK (Te Vavisz), VIO5 Zsiepiyis Rybiizpege (a., Ohio) and in ""SK Ariisaiopi5

Mapia!i, Za Eyaop (2006), Kospe Oiadpobis5 StrN, Mappeite or mmlu.gosNe-arriiea-vsieepse.sot".

It should be understood that it may be necessary to correct a number of parameters of any laboratory protocol, for example, PCR-identification protocols in the following

Examples, to laboratory-specific conditions and their minor modification to obtain similar results. For example, when using different methods of DNA preparation or choosing other primers, different optimal conditions for the PCR protocol may be required. Together with 60, these correction options are obvious to specialists and, moreover, are described in detail in modern guidelines for the use of PCR.

The following sequences are referenced in the description and examples:

ZEOIO Mo.1: 0/0 fragment from Zai! of the nucleotide sequence of the rzyen10 vector.

ZEO IO Mo. 2: nucleotide sequence including the 5' region flanking the foreign

DNA including herbicide resistance genes in EE-CM3.

ZEO IO Mo. 3: nucleotide sequence including the 3' region flanking the foreign

DNA including herbicide resistance genes in EE-CM3.

ZEO and Mo. 4: primer 5OU028

ZEOIO Mo.5: 0 primer 50U029

ZEO IO Mo. 6: primer ZMP187

ZEOIO Mo.7: primer 5ТМ019

ZEO IO Mo. 8: nucleotide sequence of the amplicon

ZEOIO Mo.9: primer 1 for amplification of the control fragment (ZOMO1)

ZEOIO Mo.10: primer 2 for amplification of the control fragment (ZOM02)

ZEOIO Mo.11: nucleotide sequence pB2/P355AsK

ZEOIO Mo.12: nucleotide sequence including a 5' region flanking a foreign

DNA in EE-SMI1

ZEOIO Mo.13: a nucleotide sequence including a 3' region flanking a foreign

DNA in EE-SMI1

ZEOIO Mo. 14: Nucleotide sequence including the 5' region flanking the foreign

DNA in EE-0aM2

ZEOIO Mo. 15: Nucleotide sequence including the 3' region flanking the foreign

DNA in EE-0aM2

ZEOIO Mo.16: primer 5OU06

ZEOIO Mo.17: primer 5OKHU07

ZEOIO Mo.18: primer 5OU09

ZEOIO Mo.19: primer 5ОХО010

ZEOIO Mo.20: nucleotide sequence of foreign DNA and plant linking sequences in EE-YAMZ

ZEOIO Mo.21: primer ZNA130

ZEOIO Mo.22: primer ZNA178

Examples 1. Transformation of Siusipe tah with herbicide resistance genes. 1.1. Description of foreign DNA, including chimeric genes 2ptERZRB and NRPO-RI-M/336

Plasmid poE10 is a cloning vector derived from ROS19, containing the chimeric gene 2terzr5 and the chimeric gene prra-Pi-M/336, located in a fragment of ZaiYi with a size of approximately 7.3 kbp. A complete description of the DNA contained between the two restriction sites is given below in

Tables 1. Nucleotide sequence presented in ZEO IO Mo: 1.

Table 1

The position of nucleotides in the DNA contained between the restriction sites of ZaiY in pOE10 (ZEO IO Mo 1)

Z'po5: the sequence including the 3'- complementary untranslated region of the nopaline synthase gene 188-479 chain with T-DNA ptiT37 Adgobasiegit ishptegasivep5 (OerisKeg evi a!., 1982, Uoigpai oi MoiIesshag apa

Arriiei Sepviisv, 1, 561-573

Prraghii mu336: the coding sequence of 4-hydroxyphenylpyruvate dioxygenase strain 480-1556 complementary Rzeidotopavkh Pyogesepsz AZ2, the chain is modified by replacing the amino acid glycine-336 with tryptophan, according to the description in Votsades ei a!I. (2001), US patent O5Z624596881

TRoir U: the coding sequence of an optimized derived transition peptide (tyrosine replacement at position 55), which is complementary to the sequence of the small subunit genes 1557-1928 chain ribulose-1, 5-bisphosphate carboxylase/oxygenase 7ea tauv (maize) and NePapipiz appiiv5 ( sunflower), according to the description in I ebgyp ei ai. (1996) 55510471

Beh: a sequence comprising the leader 1929-2069 complementary sequence of the tobacco engraving virus chain as described in Saitipdiop apa Egeeyei 1990) Choshtpayi oi Migoiodu, 64, 1590-1597

Ri4a748 ABVS: a sequence comprising the complementary promoter region of the histone gene NA Agabridorziv 2070-3359 chain of INaiiap, including an internal duplication (Sparocie ei a!., 1987) Reapi MoiIesshiag Wiiodu, 8, 179-191.

Ri4a748: sequence including 3360-4374 promoter region of histone NA gene of Agabridorziv

Inaiiiiapa (Sparocie ei a!., 1987) Riapi MoiIesshiag

Vioiodu, 8, 179-191. ipigop1 PZAES the first intron of the II variant of histone NZ gene. PI Agabidorziv Ipaiapa (Spatsbeyi ei 4375-4855 a!., 1992) hildtai oi Moiesshag Vioiodu, 225, 569-574.

TRoir C: the coding sequence of an optimized transition peptide containing the gene sequence of the ribulose-1 small subunit, 5-4856-5227 biphosphate carboxylase/oxygenase 7ea tauv (maize) and Neiapipy5 appyiv5 (sunflower), according to the description in I ebgyp ei ai . (1996) 55510471 2terzr5: the coding sequence of the double 5228-6565 mutant gene of 3-phosphate synthase 7ea tauz (maize) (Hebgyp et al., 1997) ММО9704103-А 1

Z'piviopAi: a sequence that includes the 3'- untranslated region of the histone gene NA 6566-7252 Agabidorviv INnaiapa (Sparotsshiye eyi a!., 1987)

Riapi MoiIeyesshiag Vioiodu, 8, 179-191. 1.2. Event EE-SMZ3

A Z-aligned fragment of ROE10 purified by HPLC with a size of approximately 7.3 bp. (containing the gene for resistance to glyphosate 2"ERZRZ and the gene for resistance to HPRO inhibitors HPRO-

RI-M/336) was used to obtain transformed soybean plants by direct gene transfer into soybean cells of the Chask type (Mmiskei!I, S. O., 5. K. Moei, O. U. Tpotav, apa V. U/aiIeg.

Kedivigayop oi "ask" v5oureap. Page Zsi 1365. 30.1990) with subsequent regeneration of transformed plant cells into transgenic fertile soybean plants. 1.2.1. Identification of the elite event EE-SMZ3

Selection of the elite event EE-ZM3 was performed based on a large-scale selection procedure based on the high level of expression and stability of herbicide resistance genes, and its compatibility with optimal agronomic characteristics, for example, plant height, height to node, resistance to lodging, growth, yield Plants containing this event were selected from a wide range of different transformation events produced using the same chimeric genes. The parameters used in the selection of this event were: a) acceptable resistance to the use of the herbicide isoxaflutol in field tests, b) acceptable resistance to the use of the herbicide glyphosate in field tests, c) acceptable resistance to the combined use of the herbicides isoxaflutol and glyphosate in field tests, d) inclusion of transgenes of resistance to herbicides in a single locus of the genome of a soybean plant in the absence of a vector sequence, e) similarity of general agronomic characteristics with the original plants used in transformation (ripening, dormancy, susceptibility to diseases, etc.), and f) absence of significant loss yield due to the insertion of the transforming DNA (compared to an isogenic line that does not contain the event, e.g. a line of plants used for transformation grown under the same conditions).

From the TZ generation, a homozygous soybean line was selected for the EE-OM3 transformation event to obtain seeds. In the areas of adaptation of the initial variety Chuask, multi-district field research was conducted in several repetitions. Field trials included herbicide resistance and agronomic performance. It was found that the agronomic productivity of plants containing EE-

SMU3 was comparable to the Asc variety (in the absence of herbicides).

Field tests also showed that plants carrying EE-CM3 had: - plant morphology and seed characteristics similar to the Chuask variety, - no changes in response to soybean diseases compared to the ask variety, and - no changes in germination or seed dormancy compared to the variety Chuask.

Seeds (generation T1 or 51), collected from the original transformant (TO) (plants transformed with the construct for the purpose of obtaining the EE-ZM3 event) in the vegetation house, were planted in the field. Three plots were sown and treated with glyphosate in the amount of 0.2 or 4 kg/ha.

Seeds were collected from plants showing the desired level of resistance to the herbicide, glyphosate.

Seeds (generation 12) collected from self-pollinated T1 plants grown in the field were sown on a "plant per row" basis. Split analysis of data within stands (fully or partially resistant) and individual plants within stands (resistant or susceptible) using the chi-square test demonstrated the expected Mendelian inheritance of the single insertion in EE-M3.

The selection and accumulation of seeds continued until the determination of the homozygosity of the line for the transformation event EE-SOM3Z and its selection for obtaining seeds in the fourth generation.

Next, the seeds of the T5 generation were used as a candidate for the development of various varieties. Sixth generation plants (generation tb) were crossed with standard soybean lines for crossing under an introgression program designed to transfer the event to an expanded basis

From the germplasm of commercial soybeans. E1 hybrid plants (EE-5M3 lines x standard lines) were grown to maturity and E2 seeds were planted. Leaf samples of 901 E2 plants were analyzed using PCR primers designed to determine the zygosity of the EE-

OSM3. The expected, according to Mendel's laws, the cleavage ratio of a single insertion of 1:2:1 was observed.

A selected event of EE-CM3 was included in different commercial genetic environments and the results of field trials in different areas were compared. The resistance of plants to the herbicide glyphosate and/or the herbicide isoxaflutol was evaluated under different treatment options. The plants showed good resistance to herbicides. Inheritance was studied using hundreds of different soybean cultivars containing the EE-OMZ event and applying herbicides. The lines isolated from these tests were then propagated in the field and also treated with herbicide. As a result of these tests, 50 selected lines were propagated and treated with herbicide. Phytotoxicity scores for the latter lines sprayed with isoxaflutol and glyphosate showed some variability in response, however, the range of responses among lines displayed variability similar to that observed among the 4 replicates of the EE-5M3 event in the original uUask environment grown under the same treatment regime and conditions environment. Thus, in plants containing EE-CM3, resistance to relevant herbicides was observed among a wide range of germplasm.

In addition, plants containing the EE-ZM3 event had normal leaf, flower, and pod morphology, excellent fertility, and showed no abnormal susceptibility to disease or insects in different genetic environments. During introgression into different variants of the genetic environment, no problems with violations or anomalies were found.

In one of the seasons, a study was carried out in 10 districts, designed to compare the agronomic productivity of soybeans, which contained the transformation event EB-ZZMZ3 and was characterized by double resistance to herbicides, with different original transformed varieties - the Wask variety and some non-transgenic soybean varieties . EE-YAMZ plants were grown in replicate plots using random plotting and/or standard weed control measures, or designated herbicides such as glyphosate and isoxaflutol. Areas with soybean plants containing the transformation event EE-CM3 were sprayed with the herbicide isoxaflutol at a planned consumption of 70 grams of the active component/ha and with the herbicide glyphosate - at a planned consumption of 1060 grams

From the active component/ha. The application of the herbicide to the mentioned plants was carried out in the form of a foliar aerosol approximately at the M4-M5 plant growth stage. Agronomic observations were carried out at the beginning, during and at the end of the season. The density of plant cover (parameter: the number of upright plants) for the plot with the asc variety and non-transgenic varieties exceeded the density in the plots with the EE-2M3Z event by a value equal to one standard deviation. The early difference in the number of upright plants was a result of the quality of the seed lot, as the planting seeds of plants containing EE-OM3 were obtained in the incubator during the opposite season, while the seeds of the non-transgenic cultivars were obtained during the continuous normal growing season under US conditions. At the same time, the number of days to reach 50-95 germination and plant power indicators were the same, which indicated the comparability of the seed lots in terms of these parameters of productivity. When counting upright plants at the end of the season, the difference between the ChaskK variety and non-transgenic varieties from plants with

EE-OMZ by the value of one standard deviation. The yield of plants with an EE-SMZ event from a plot was also lower than that of the cultivar UaskK by one standard deviation, possibly as a result of lower plant density in plots with plants containing an EE- event.

CM3. The yield of the non-transgenic varieties was higher than that of the Chdask variety, which could be expected due to the improvement of yield potential in modern varieties.

In the course of one of the tests, plant viability indicators were evaluated at three stages of growth: M4-5, 1 and full maturity. The first evaluation was carried out soon after the planned application of herbicides. At the final viability assessment, plants containing EE-CM3Z sprayed with both herbicides had the same performance as untreated Wasc plants or untreated plants containing EE-OM3. According to assessments made by agronomists, plants sprayed with herbicides at growth stages M4-5 and K1 received a vitality rating of 3-4 (moderate damage). Unsprayed plants (non-transformed Chask plants or soybean plants containing EE-ZM3) received a score of 4.6-4.8 (a score of 5 means no damage). At the last assessment of plant viability, all sites received the same assessment, level 5 (absence of PoShshKODzheny).

One of the test seasons was distinguished by a particularly high amount of precipitation, and the effect of the herbicides intended for treatment on crops containing EE-ZM3 was more evident than in

From other seasons. Field trials also included monitoring of fitness characteristics (breeding, disease resistance, fertility, seed dispersal, dormancy, survival). The reproduced characteristics - the number of days to germination, the number of days to 50 95 flowering, and the number of days to 90 95 bean ripening - in plants that contained EE-OMZ and plants of the variety

Uask did not differ. There were no discrepancies in the response to natural infection by plant diseases and insect pests. Despite the fact that EE-53M3 yielded less than the UaskK variety, no differences in fertility (weight of 100 seeds) were found. The assessment of seed dispersal parameters (destroying of beans and lodging of plants) showed that EE-CM3Z and dask plants have the same rate of destruction of beans, however, EE-CM3 plants had a lower tendency to lodging. Evaluation of seeds collected from 10 districts revealed no problems with germination or seed dormancy evaluation.

In trials during a season with particularly high rainfall, the final yield of plants with the EE-ZM3 event, regardless of the weed control method, was lower than that of the cultivar UaskK by one standard deviation (perhaps as a result of the lower density plants in plots with plants that contained the EE-CM3 event).

During a particularly wet season, crop damage was described (burning up to 10-30 95 of the crop area) in plots with EE-SMZ3 plants within six weeks after foliar treatment with the herbicides glyphosate and isoxaflutol. However, after ripening, all plots were assigned a plant viability assessment of "no damage". Repeated multisite field trials of plants with EE-CM3 incorporated into an elite soybean environment compared with near-isogenic sister lines that did not contain the transgene were expected to show no yield differences between plants containing the EE-CM3 event and near-isogenic lines (in the absence of treatment with herbicides).

In addition, during repeated field tests, a significant resistance of crops (burning less than 10 95) of soybean plants containing EE-OM3 was found, with pre- or post-emergence IT treatment (70 g of active component/ha with 0.5 95 MI5, Adhidekh), as well as significant crop resistance (burning less than 10 95) of soybean plants containing EE-CM3, with post-emergence treatment with pyrasulfotol (35 g of active component/ha with 0.5 95 MI5, Adiideh) - another herbicide-inhibitor of NRPO. 1.2.2. Identification of flanking regions of foreign DNA of elite event EE-2MZ3

The sequence of the regions flanking the foreign DNA, which includes genes for resistance to 60 herbicides in the elite event EE-M3, was determined as follows:

1.2.2.1. Right (5) flanking area

The fragment identified as containing the 5'-linking region was sequenced; its nucleotide sequence is presented in 5EO IUO Mo 2. The sequence between nucleotides 1 and 1451 corresponded to plant DNA, while the sequence between nucleotides 1452 and 1843 corresponded to alien DNA. 1.2.2.2. Left (3) rlinking section

The fragment identified as containing the 3'-flanking region was sequenced; its nucleotide sequence is presented in 5EO IUO Mo 3. The sequence between nucleotides 1 and 240 corresponded to alien DNA, while the sequence between nucleotides 241 and 1408 corresponded to plant DNA. 1.2.2.3. Foreign DNA containing genes for resistance to herbicides EE-JOMZ3

With the help of various molecular methods, it was determined that the foreign DNA of the elite event EE-

CM3, which included the herbicide resistance genes, contained two partial 3'-sequences of pPisiopAi in a "head-to-head" orientation, followed by two almost complete copies of the zI fragment of roE10 in a "head-to-tail" orientation (see Figure 1).

Foreign DNA containing EE-ZM3 herbicide resistance genes thus contained the following sequences in order: - from nucleotide 1 to nucleotide 199: the nucleotide sequence corresponding to the complementary strand of the nucleotide sequence "BO IUO Mo 1 from nucleotide 6760 to nucleotide 6958 ; - from nucleotide 200 to nucleotide 624: the nucleotide sequence that corresponded to the nucleotide sequence of ZEO IO Mo 1 from nucleotide 6874 to nucleotide 7298; - from nucleotide 625 to nucleotide 7909: the nucleotide sequence that corresponded to the nucleotide sequence of ZEO IO Mo 1 from nucleotide 7 to nucleotide 7291; - from nucleotide 7910 to nucleotide 15163: the nucleotide sequence that corresponded to the nucleotide sequence of ZEO IO Mo 1 from nucleotide 12 to nucleotide 7265; and - from nucleotide 15164 to nucleotide 15187: the nucleotide sequence that corresponded to the nucleotide sequence of ZEO IUO MoZ from nucleotide 217 to nucleotide 240 (this sequence does not correspond either to the plasmid DNA of poE10 or to the DNA of the wild-type plant and therefore

Zo is called filler-DNA).

Directly above this foreign DNA was preceded by a continuous 5'-flanking sequence ZEO IO Mo 2, from nucleotide 1 to 1451, and directly below this foreign DNA

The DNA was located continuous with the 3'-flanking sequence 5EO IU Mo 3, from nucleotide 241 to nucleotide 1408.

Confirmed complete sequencing of foreign DNA and flanking DNA sequences in EE-

CM3 made it possible to obtain the sequence presented in 5EO IU Mo 20. In this sequence, the included DNA was located from position 1452 to position 16638, and two almost complete copies of P2E10 were located in the "head-to-tail" orientation from position 2257 to position 16601. 5'-linking the DNA sequence in 5EO IU Mo 20 was the sequence from position 1 to position 1451 in 5EO IU Mo 20, and the 3'-linking DNA sequence in 5EO IU

Mo 20 was a sequence from position 16639 to position 17806 in 5EO IU Mo 20. 1.3. Description of foreign DNA including chimeric phosphinothricine acetyltransferase genes

Plasmid pB2/P355AcSK was a cloning vector based on pOS19, which contained a chimeric rai gene. The description of the vector is given below in Table 2. Its nucleotide sequence is presented in 5EO IU Mo 11.

Table 2

The position of the nucleotides of the components of rB2/RZ55АсСК (ZEO IU Mo 11)

SHI Ve eh pinni yiovi Y 1012-1563 . mipidosptotodepev 1.4. Event EE-b.M1 1.4.1. Identification EE- M1

Soybeans resistant to herbicides were developed by transforming soybeans with the rB2/RZ355АСК vector using direct gene transfer.

Selection of the elite event EE-Z2MI1 was performed based on a large-scale selection procedure based on the high level of expression and stability of the herbicide resistance gene, as well as its compatibility with optimal agronomic characteristics. 1.4.2. Identification of flanking regions and foreign DNA of the EE-CM1 elite event 1.4.2.1. Right (5) flanking area

The fragment identified as containing the 5'-linking region was sequenced; its nucleotide sequence is presented in 5EO IU Mo 12. The sequence between nucleotides 1 and 209 corresponded to plant DNA, while the sequence between nucleotides 210 and 720 corresponded to alien DNA. 1.4.2.2. Left (3) flanking area

The fragment identified as containing the 3'-flanking region was sequenced; its nucleotide sequence is presented in 5EO IU Mo 13. The sequence between nucleotides 1 and 568 corresponded to alien DNA, while the sequence between nucleotides 569 and 1000 corresponded to plant DNA. 1.4.2.3. Foreign DNA containing genes for resistance to EEM herbicides

With the help of various molecular methods, it was determined that the foreign DNA of the elite event EE-

The medium that included the herbicide resistance gene contained two copies of the chimeric raya gene in the form of a direct repeat (see Figure 3).

Foreign DNA containing EE-ZM'I1 herbicide resistance genes thus contained the following sequences in order: - from nucleotide 1 to nucleotide 3122: the nucleotide sequence corresponding to the nucleotide sequence of ZEO IO Mo 11 from nucleotide 340 to nucleotide 3461; - from nucleotide 3123 to nucleotide 3458: the nucleotide sequence corresponding to the complementary chain of the nucleotide sequence "EO IU Mo 11 from nucleotide 1 to nucleotide 336; - from nucleotide 3459 to nucleotide 4073: the nucleotide sequence that corresponded to the complementary chain of the nucleotide sequence of BEO IU Mo 11 from nucleotide 3462 to nucleotide 4076; and - from nucleotide 4074 to nucleotide 6780: the nucleotide sequence corresponding to the nucleotide sequence of ZEO IO Mo 11 from nucleotide 337 to nucleotide 3043; and - from nucleotide 6781 to nucleotide 6790: the nucleotide sequence that corresponded to the nucleotide sequence of ZEO IUO Mo 13 from nucleotide 559 to nucleotide 568 (this sequence does not correspond to either plasmid DNA or wild-type plant DNA and is therefore called filler DNA).

Directly above this foreign DNA EE-MDI was preceded by a continuous 5'-flanking sequence ZEO IUO Mo 12, from nucleotide 1 to 209, and directly below this foreign DNA was a continuous 3'-flanking sequence 5EO IUO Mo 13 from nucleotide 569 to nucleotide 1000. 1.5. Event EE-S0M2

Soybeans resistant to herbicides were developed by transforming soybeans with the rB2/RZ355АСК vector using direct gene transfer.

Selection of the elite event EE-2M2 was performed based on a large-scale selection procedure based on the high level of expression and stability of the herbicide resistance gene, as well as its compatibility with optimal agronomic characteristics. 1.5.1. Identification of flanking sites and foreign DNA of elite event EE-24M2 1.5.1.1. Right (5) flanking section

The fragment identified as containing the 5'-linking region was sequenced; its nucleotide sequence is presented in 5EO IU Mo 14. The sequence between nucleotides 1 and 311 corresponded to plant DNA, while the sequence between nucleotides 312 and 810 corresponded to alien DNA. 1.5.1.2. Left (3) rlinking section

The fragment identified as containing the 3'-flanking region was sequenced; its nucleotide sequence is presented in 5EO IU Mo 15. The sequence between nucleotides 1 and 507 corresponded to foreign DNA, while the sequence between nucleotides 569 and 1880 corresponded to plant DNA. 1.5.1.3. Foreign DNA containing genes for resistance to herbicides ЕЕ-ЛМ2

With the help of various molecular methods, it was determined that the foreign DNA of the elite event EE- or OM2, which included the gene for resistance to herbicides, contained one copy of the chimeric gene of paradise (see

Figure 4).

Foreign DNA containing EE-ZM2 herbicide resistance genes thus contained the following sequences in order: - from nucleotide 1 to nucleotide 391: a nucleotide sequence that corresponded to the nucleotide sequence of ZEO IO Mo 11 from nucleotide 3458 to nucleotide 3848; and - from nucleotide 392 to nucleotide 3436: the nucleotide sequence that corresponded to the nucleotide sequence of ZEO IO Mo 11 from nucleotide 413 to nucleotide 3457.

Directly above this foreign DNA EE-2M2 was preceded by a continuous 5'-flanking sequence ZEO IO Mo 14, from nucleotide 1 to 311, and directly below this foreign DNA was a continuous 3'-flanking sequence 5EO IUO Mo 15 from nucleotide, 508 to nucleotide 1880. 2. Development of PCR identification protocol for EE-CM3. 241. Primers

Specific primers have been developed that recognize sequences within the elite event.

A primer was developed that recognizes the sequence within the 3'-flanking region of EE-CM3.

The second primer was selected within the sequence of the foreign DNA, so that the primers overlapped a sequence of approximately 263 nucleotides. It was found that the following primers made it possible to obtain the most clear and reproducible results during PCR with DNA

EE-OM3: zOHO28: 5-ATS.asSt.TTAAsSa.tob.sSto.Aa-3 (target: included DNA) (5EO IO Mo: 4) zOUO29: 5-SAA.dasS.Sto.aA9d.ATT.ATO- 3" (target: plant DNA) (EF IO Mo: 5)

Primers, the target of which was the endogenous sequence, were preferably included in the mixture for

PCR. These primers were internal controls for unknown samples and positive controls

DNA. The positive result obtained when using a pair of endogenous primers (the presence of a PCR-amplified fragment with a size of 413 p.p.) demonstrated that it is possible to obtain a sufficient amount of a PCR product - DNA of appropriate quality from the preparation of genomic DNA. Endogenous primers were chosen that recognized the endogenous soybean actin gene: 5ОЧО1 5-атС.АдС. SAS АСА.ЯТ9.ССТ.АТ-З" (ZEO IYU Mo: 9) zОохо2 5-аTT.АСС.ЯаТА.САдатс.ттт.оо-3' (ZEO IYU Mo: 10) 2.2. Amplified fragments

The expected PCR amplified fragments were:

For a pair of primers ZHOM01-50502: 413 p.o. (endogenous control)

For a pair of primers ZXH0U028-505029: 263 p.o. (EE-CM3 elite event) 2.3. Matrix DNA

Matrix DNA was isolated from a leaf fragment obtained by composting, according to Eduagaz

Oh! (MysiIvis Asii Kezeagsp, 19, p1349, 1991). If DNA isolated by other methods was used, it was necessary to perform a test run using different amounts of matrix.

Usually, the best results were obtained when using 50 ng of genomic template DNA. 2.4. Appointed positive and negative control

In order to avoid false positive or negative results, it was determined that the following positive and negative controls should be included in the PCR run: - Master mix control (negative DNA control). This is PCR, during which DNA was not added to the reaction mixture. If the expected result was obtained - the absence of a PCR product - this meant that the PCR mixture was not contaminated with the DNA target. - Positive DNA control (genomic DNA sample that deliberately contained transgenic sequences). Successful amplification of the positive control demonstrated that the PCR was performed under conditions that provided amplification of the target sequences. - Wild-type DNA control. This is PCR, during which the DNA matrix was genomic DNA isolated from a non-transgenic plant. If the expected result was obtained - the absence of amplification of the transgene PCR product when the endogenous PCR product was amplified - this meant that there was no detectable background amplification of the transgene in the genomic DNA sample. 2.5. PCR conditions

Optimal results were obtained under the following conditions (It is understood that when describing different conditions for optimal results, examples of such conditions are presented. It is clear that a specialist can change conditions, reagents and parameters, for example, use other Tad polymerases, and achieve the desired results): - the PCR mixture for a reaction mixture volume of 25 μl contained: 20 ng of template DNA 2.5 μl of 10x amplification buffer (supplied by the manufacturer with Tad polymerase)

0.5 μl 10 mm aMTP 0.4 μl 5001 (10 pmol/μl) 0.4 μl 50702 (10 pmol/μl) 0.7 μl 50028 (10 pmol/μl) 0.7 μl 50U029 (10 pmol/μl) ) 0.1 μl of Tad-DNA polymerase (5 units/μl) water to a volume of 25 μl - the thermocycling profile that allowed obtaining optimal results looked like this: 4 min. at 9570

Then: 1 min. at 9570 1 min. at 57 "C for 2 min. at 72"7C

During 5 cycles

Then: 30 spri 9270 30 spri 57 "С 1 min. at 72"7С

During 25 cycles

Then: 10 minutes at 727 2.6. Analysis in agarose gel

For optimal visualization of PCR results, it was determined that 10 to 20 μl of PCR samples should be applied to a 1.5 95 agarose gel (Tris-borate buffer) with an appropriate molecular weight marker (for example, a 100 p.o. Rpagtasia ladder). 2.1. Confirmation of results

It was determined that data obtained from DNA samples of transgenic plants using one PCR run and one PCR mixture should not be considered acceptable if 1) the positive DNA control did not allow obtaining the expected PCR products (transgenic and endogenous fragments), 2) the negative DNA control was not negative by PCR amplification (the absence of fragments was not confirmed) and 3) wild-type DNA control did not allow obtaining the expected results (amplification of the endogenous fragment).

Following the PCR Identification Protocol for EE-CM3 as described above, visible amounts of transgene and endogenous PCR products of the expected size in the lanes indicated that the respective plant from which the genomic template DNA was isolated had inherited the EE-OM3 elite event. If there was no visible amount of transgenic products in the lanes

Visible amounts of endogenous PCR products were present from the PCR, indicating that the respective plant from which the genomic template DNA was isolated did not contain the indicated elite event.

If there was no visible amount of endogenous and transgenic PCR products in the lanes, this indicated that the quality and/or quantity of genomic DNA did not allow for a PCR product.

Calculating grades for these plants was not possible. It was necessary to repeat the isolation of genomic DNA and perform a new PCR run with appropriate controls. 2.8. The use of a differential PCR protocol for the identification of EE-АФМЗ

Before attempting to screen unknown samples, a test run with appropriate controls was performed. The developed protocol may require optimization of components, which may differ in different laboratories (isolation of template DNA, Tad DNA polymerase, quality of primers, aMTP, thermal cycler, etc.).

Amplification of the endogenous sequence played a key role in this protocol. It was necessary to achieve PCR and thermocycling conditions during which amplification of equimolar amounts of both endogenous and transgenic sequences of known transgenic matrix genomic DNA is possible. In all cases when the amplification of the endogenous target fragment did not occur or when the amplification of the target sequences did not occur, according to electrophoresis in agarose gel with the same intensity of ethidium bromide staining, optimization of the PCR conditions could be required.

According to the protocol described above, the leaf material of a number of soybean plants, some of which contained EE-ZM3, was tested. Samples of the elite event EE-ZMZ and wild-type soybeans were used as positive and negative controls, respectively.

Figure 2 shows the result obtained when using the Protocol of PCR amplification of the elite event EE-ZM3Z and a number of samples of soybean plants. The samples in lanes 2 and C were found to contain the elite event EE-OM3, as a band corresponding to 263 bp was detected, while the samples in lanes 4-8 did not contain EE-ZM3. Lanes 6 and 7 contained samples of other soybean transformation events obtained using the same chimeric herbicide resistance genes; lane 8 contained DNA from wild-type soybean plants, and lane 9 was a negative control sample (water), lanes 1 and 10 were a molecular weight marker (100 p.p. ladder). 2.9. Detection of EE-CM3Z using dPCR analysis in a bulk batch of seeds

Differential PCR analysis (dDPuCR) was set up to detect a low level of EE-OMZ in a bulk batch of seeds. Under reproducible conditions, the minimum successful level of transgenic seed in a bulk lot of non-transgenic seed was 0.495 (wt/wt). Thus, it was determined that the limit of detection was 0.4 95 (mass/mass).

The following primers were used for targeted PCR:

A forward primer targeting the T-DNA sequence:

ZNA130 5-STA.TAT.TST.a9dT.TSS.AAT.TTA.TO-3" (BEO IYU Mo 12)

Reverse primer targeting the 3'-flanking sequence:

ZMRI178 5-TaA.doaS.ASA.TAT.TOA.TdA.SS-3" (BEO IYU Mo 13)

The size of the expected amplified fragment by PCR using these primers was 115 bp.

Targeted PCR was performed using approximately 200 ng of template DNA isolated from soil-soaked seeds according to the instructions of the modified DNA isolation and purification kit of Sepiga Rygedepe OMA rigiyisanop ehigasiyup Kiyi (Oiadepe). If used

DNA isolated by other methods was required to perform a test run using known relative levels of EE-CCM3.

A validated PCR with a reference system targeting an endogenous sequence ideally needed to be performed as a separate PCR run to confirm the suitability of the DNA sample for PCR analysis to avoid false negatives.

When using unknown tested samples, the PCR experiment should ideally include appropriate samples of positive and negative controls, i.e.: - Master mix control (negative DNA control). This is PCR, during which DNA was not added to the reaction mixture. If the expected result (absence of PCR product) was obtained for both target and reference systems, this meant that the PCR mixture was not contaminated with target DNA. - Positive DNA control (genomic DNA sample that deliberately contained transgenic sequences). Successful amplification of the positive control demonstrated that the PCR was performed under conditions that provided amplification of the target sequences. - Wild-type DNA control could also be introduced into this PCR. This is PCR, during which the DNA matrix was genomic DNA isolated from a non-transgenic plant. If the expected result was obtained - the absence of amplification of the transgene PCR product when the endogenous PCR product was amplified - this meant that there was no detectable background amplification of the transgene in the genomic DNA sample.

Optimal results were obtained under the following conditions: - the PCR mixture for a reaction mixture volume of 25 μl contained: 200 ng of matrix DNA 5 μl of 5x working buffer solution 0.25 μl of 20 mM aMTP 0.7 μl of ZNA130 (10 pmol/l) 0, 4 μl of ЗНА178 (10 pmol/l) 0.1 μl of 5O-Tad-DNA polymerase (5 units/l) water to a volume of 25 μl - the thermal cycling profile that allowed obtaining optimal results looked like this: 4 min. at 9570

Then: 1 min. at 9570 1 min. at 57 "C for 2 min. at 72"7C

During 5 cycles

Then: 30 spri 9270 30 spri 57 "С 1 min. at 72"7С

During 30 cycles

Then: 10 minutes at 727

For optimal visualization of the PCR results, it was determined that 25 μl of the PCR product needed to be applied to a 1.5 95 agarose gel (Tris-borate buffer) with an appropriate molecular weight marker (for example, a 50 p.p. ladder).

Following the PCR method as described above, the visible amounts of PCR products of the target and reference systems of the expected size in the lanes indicated that the tested sample from which the genomic template DNA was isolated contained a level of the elite event EE-CM3Z that exceeded the detection limit of the target reactions

If the lanes lacked visible amounts of target PCR products and were present in visible amounts of reference PCR products, this indicated that the test sample from which the genomic template DNA was isolated contained a level of the EE-ZM3 elite event below the detection limit target reaction.

If there was no visible amount of endogenous and transgenic PCR products in the lanes, this indicated that the quality and/or quantity of genomic DNA did not allow for a PCR product.

Assessments were not performed for these plants. It was necessary to repeat the isolation of genomic DNA and perform a new PCR run with appropriate controls. 3. Using a specific integrated fragment as a probe to detect material that included EE-CM3.

A specific integrated fragment of EE-SbOMZ was obtained by PCR amplification using specific primers 50028 (5EBO I Mo 4) and 50029 (5BO IO Mo 5), which made it possible to obtain an amplicon with the nucleotide sequence 5EO IUO Mo 8, or by chemical synthesis, and labeled . This integrated fragment was used as a specific probe for the detection of EE-OM3Z in biological samples. Nucleic acid was isolated from samples according to standard procedures. This nucleic acid was then contacted with a specific probe under hybridization conditions optimized for hybrid formation. Detection of hybrid formation indicated the presence of EE-ZMZ nucleic acid in the sample. Optionally, the nucleic acid in the samples was amplified before contact with a specific probe using specific primers. As an alternative instead of an integrated fragment

Nucleic acid was labeled before contact with a specific probe. Optionally, the specific probe was attached to a solid support (eg, but not limited to, a filter, strip, or pellet) prior to contact with the samples. 4. PCR-identification protocol for EE-SM'1. 4.1. Primers

We developed specific primers that recognize sequences within the elite event.

In particular, we developed a primer that recognizes the sequence within the 5'-flanking region of EE-

mass media The second primer was chosen within the sequence of the foreign DNA, so that the primers overlapped the sequence of approximately 183 nucleotides. It was found that the following primers made it possible to obtain the most clear and reproducible results during PCR with

DNA EE-OMI: 5006 5' -d9S.97T.Sat.A9T.dAS.TdA.Yad-3" (ZEO IO Mo: 16) (target: plant DNA) 5007 5 -9TT.TTA.SAA.Sat.SataAs . Tda-3" (ZEO IO Mo: 17) (target: included DNA)

Primers, the target of which was the endogenous sequence, were preferably included in the mixture for

PCR. These primers were internal controls for unknown samples and positive controls

DNA. The positive result obtained when using a pair of endogenous primers demonstrated that it is possible to obtain a sufficient amount of PCR product - DNA of appropriate quality from the preparation of genomic DNA. Endogenous primers were chosen that recognized the housekeeping gene in Syusipe tah: 50U01 5 -dgS.AaS.САС.АСА.атТа.ССтТ.АТ-3" (5EO IYU Mo: 9) (located in the actin 1 gene of Syusipe tah (Accession number 9U01298))

5002 5' -9TT.ASS.YATA.СА9Я.ato.ТтТ.со-3" (ZEO IO Mo: 10) (located in the actin 1 gene of Siusipe tah (Accession number 9U01298)) 4.2. Amplified fragments

The expected PCR amplified fragments were:

For a pair of primers 5OU01-507502: 413 p.o. (endogenous control)

For a pair of primers 50U06-50507: 183 p.o. (elite event EE-SM'1) 4.3. Matrix DNA

Matrix DNA was isolated from a leaf fragment obtained by composting, according to Edumagaz et al. (Misieis Asii Kezeagsp, 19, p1349, 1991). If DNA isolated by other methods was used, it was necessary to perform a test run using different amounts of matrix.

Usually, the best results were obtained when using 50 ng of genomic template DNA. 4.4. Appointed positive and negative control

In order to avoid false positive or negative results, it was determined that the following positive and negative controls should be included in the PCR run: - Master mix control (negative DNA control). This is PCR, during which DNA was not added to the reaction mixture. If the expected result was obtained - the absence of PCR products - this meant that the PCR mixture was not contaminated with the DNA target. - Positive DNA control (genomic DNA sample that deliberately contained transgenic sequences). Successful amplification of the positive control demonstrated that the PCR was performed under conditions that provided amplification of the target sequences. - Wild-type DNA control. This is PCR, during which the DNA matrix was genomic DNA isolated from a non-transgenic plant. If the expected result was obtained - the absence of amplification of the transgene PCR product when the endogenous PCR product was amplified - this meant that there was no detectable background amplification of the transgene in the genomic DNA sample. 4.5. PCR conditions

Optimal results were obtained under the following conditions: - the PCR mixture for a reaction mixture volume of 25 μl contained: 2.5 μl of template DNA 2.5 μl of 10x amplification buffer (supplied with Tad polymerase) 0.5 μl of 10 mM aMTP

Zo 0.5 μl 5006 (10 pmol/μl) 0.5 μl 5007 (10 pmol/μl) 0.25 μl 5001 (10 pmol/μl) 0.25 μl 5002 (10 pmol/μl) 0.1 μl Tad - DNA polymerases (5 units/μl) 35 water to a volume of 25 μl - the thermocycling profile that allowed to obtain optimal results looked like this: 4 min. at 9570

Then: 1 min. at 9570 1 min. at 57 "C for 2 min. at 72"7C

During 5 cycles 40

Then: 30 spry 9270 spry 57 "С 1 min. at 72"7С

During 25 cycles

Then: 5 minutes at 7270 4.6. Analysis in agarose gel

For optimal visualization of PCR results, it was determined that 10 to 20 μl of PCR samples needed to be applied to a 1.5 95 agarose gel (Tris-borate buffer) with an appropriate molecular weight marker (for example, a 100 p.o. Rpagtasia ladder). 4.7. Confirmation of results

It was determined that data obtained from DNA samples of transgenic plants using one PCR run and one PCR mixture should not be considered acceptable if 1) the positive DNA control did not allow obtaining the expected PCR products (transgenic and endogenous fragments), 2) the negative DNA control was not negative by PCR amplification (the absence of fragments was not confirmed) and 3) wild-type DNA control did not allow obtaining the expected results (amplification of the endogenous fragment).

Following the PCR Identification Protocol for EE-Media as described above, visible amounts of transgenic and endogenous PCR products of the expected size in the lanes indicated that the respective plant from which the genomic template DNA was isolated had inherited the elite event EE-ZM'1 . If the lanes lacked visible amounts of transgenic PCR products and were present in visible amounts of endogenous PCR products, this indicated that the respective plant from which the genomic template DNA was isolated did not contain the indicated elite event. If the lanes lacked visible amounts of endogenous and transgenic PCR products, this indicated that the quality and/or quantity of genomic DNA did not allow the PCR product to be obtained. Assessments were not performed for these plants. It was necessary to repeat the isolation of genomic DNA and perform a new PCR run with appropriate controls. 4.8. The use of a differential PCR protocol for the identification of EE-LM1

Before attempting to screen unknown samples, it was necessary to perform a test run with appropriate controls. The developed protocol may require optimization of components, which may differ in different laboratories (isolation of template DNA, Tad-DNA polymerase, quality of primers, aMTP, thermal cycler, etc.).

Amplification of the endogenous sequence played a key role in this protocol. It was necessary to achieve PCR and thermocycling conditions during which amplification of equimolar amounts of both endogenous and transgenic sequences of known transgenic template genomic DNA is possible. In all cases when the amplification of the endogenous target fragment did not occur or when the amplification of the target sequences did not occur, according to electrophoresis in agarose gel with the same intensity of ethidium bromide staining, optimization of the PCR conditions could be required.

According to the protocol described above, leaf material of a number of Siusipe tah plants, some of which contained EE-MMI1, was tested. Samples of the elite event EE-ZM'I and wild-type SiIusipe tah were used as positive and negative controls, respectively.

Figure 5 shows the result obtained when using the PCR amplification protocol of the elite event EE-SMI and a number of samples of soybean plants (lanes 1-14). The samples in lane 1 were found to contain an elite event, as a band corresponding to 185 bp was detected, while the samples in lanes 2, 3 and 4 did not contain EE-SM'I1. Lane 2 contained another soybean elite event, lane C was a non-transgenic SiIusipe tah control, lane 4 was a negative control sample (water), and lane 5 was a molecular weight marker (100 p.p. ladder). 5. Using a specific integrated fragment as a probe to detect material that included EE-SMI1.

The specific integrated fragment of EE-Z3M1 was obtained by PCR amplification using specific primers ZOMB6 (ZEO IU Mo 16) and 5007 (ZEO IUO Mo 17) or by chemical synthesis, and labeled. This integrated fragment was used as a specific probe for the detection of EE-SMI in biological samples. Nucleic acid was isolated from samples according to standard procedures. This nucleic acid was then contacted with a specific probe under hybridization conditions optimized for hybrid formation.

Detection of hybrid formation indicated the presence of EE-SM'I1 nucleic acid in the sample.

Optionally, the nucleic acid in the samples was amplified before contact with a specific probe using specific primers. As an alternative, instead of the integrated fragment, the nucleic acid was labeled before contact with a specific probe. Optionally, the specific probe was attached to a solid support (eg, but not limited to, a filter, strip, or pellet) prior to contact with the samples. 6. PCR identification protocol for EE-CM2. 6.1. Primers

We developed specific primers that recognize sequences within the elite event.

In particular, we developed a primer that recognizes the sequence within the 3'-flanking region of EE-

OM2. The second primer was chosen within the sequence of the foreign DNA, so that the primers overlapped the sequence of approximately 150 nucleotides. It was found that the following primers made it possible to obtain the most clear and reproducible results during PCR with

DNA EE-OaMO: 5ОМО95 -TatТ.99Т.ТАТ.daС.99Т.а4СС.АТО-3" (5EO ИО Mo: 18) (target: plant DNA)

5ОМО10:5 -Т9С.ТАС.Ада.САТ.Сат.997Т.49470-3" (5ЕО ИО Mo: 19) (target: included DNA)

Primers, the target of which was the endogenous sequence, were preferably included in the mixture for

PCR. These primers were internal controls for unknown samples and positive controls

DNA. The positive result obtained when using a pair of endogenous primers demonstrated that it is possible to obtain a sufficient amount of PCR product - DNA of appropriate quality from the preparation of genomic DNA. Endogenous primers were chosen that recognized the housekeeping gene in Siyusipe tah: 5ОМО15 -9ГС.АдС.САС.АСА.дТа.СсСстТ.АТ-3" (5EO IЮ Mo: 9) (located in the actin 1 gene of SiIusipe tah (Accession number U01298 )) 5ОМО25 -9ТТ.АСС.атА.СА9.ато.Ттт.0-3" (5EO IU Mo: 10) (located in the actin 1 gene of SiIusipe tah (Accession number U01298)) 6.2. Amplified fragments

The expected PCR amplified fragments were:

For a pair of primers ZHOM01-50502: 413 p.o. (endogenous control)

For a pair of primers ZHO0OM09-505010: 151 p.o. (EE-CM2 elite event) 6.3. Matrix DNA

Matrix DNA was isolated from a leaf fragment obtained by composting, according to Edumagaz et al. (Misieis Asii Kezeagsp, 19, p1349, 1991). If DNA isolated by other methods was used, it was necessary to perform a test run using different amounts of template.

Usually, the best results were obtained when using 50 ng of genomic template DNA. 6.4. Appointed positive and negative control

In order to avoid false positive or negative results, it was determined that the following positive and negative controls should be included in the PCR run: - Master mix control (negative DNA control). This is PCR, during which DNA was not added to the reaction mixture. If the expected result was obtained - the absence of a PCR product - this meant that the PCR mixture was not contaminated with the DNA target. - Positive DNA control (genomic DNA sample that deliberately contained transgenic sequences). Successful amplification of the positive control demonstrated that the PCR was performed under conditions that provided amplification of the target sequences. - Wild-type DNA control. This is PCR, during which the DNA matrix was genomic DNA isolated from a non-transgenic plant. If the expected result was obtained - the absence of amplification of the transgene PCR product when the endogenous PCR product was amplified - this meant that there was no detectable background amplification of the transgene in the genomic DNA sample.

From 6.5. PCR conditions

Optimal results were obtained under the following conditions: - the PCR mixture for a reaction mixture volume of 25 μl contained: 2.5 μl of template DNA 2.5 μl of 10x amplification buffer (supplied with Tad polymerase) 0.5 μl of 10 mM aMmtR 0, 5 µl 5009 (10 pmol/µl) 0.5 µl 50010 (10 pmol/µl) 0.25 µl 5001 (10 pmol/µl) 0.25 µl 5002 (10 pmol/µl) 0.1 µl Tad-DNA- polymerase (5 units/μl) water to a volume of 25 μl - the thermocycling profile, which allowed to obtain optimal results, looked like this: 4 min. at 9570

Then: 1 min. at 9570 1 min. at 57 "C for 2 min. at 7276

During 5 cycles

Then: 30 times 9270

30 spry 57 "С 1 min. at 72"7С

During 25 cycles

Then: 10 minutes at 727 6.6. Analysis in agarose gel

For optimal visualization of PCR results, it was determined that 10 to 20 μl of PCR samples needed to be applied to a 1.5 95 agarose gel (Tris-borate buffer) with an appropriate molecular weight marker (for example, a 100 p.p. ladder from Rpaptasia). 6.7. Confirmation of results

It was determined that data obtained from DNA samples of transgenic plants using one PCR run and one PCR mixture should not be considered acceptable if 1) the positive DNA control did not allow obtaining the expected PCR products (transgenic and endogenous fragments), 2) the negative DNA control was not negative by PCR amplification (the absence of fragments was not confirmed) and 3) wild-type DNA control did not allow obtaining the expected results (amplification of the endogenous fragment).

Following the PCR Identification Protocol for EE-CM2 as described above, visible amounts of transgenic and endogenous PCR products of the expected size in the lanes indicated that the respective plant from which the genomic template DNA was isolated had inherited the EE-CM2 elite event. If the lanes lacked visible amounts of transgenic PCR products and were present in visible amounts of endogenous PCR products, this indicated that the respective plant from which the genomic template DNA was isolated did not contain the indicated elite event. If the lanes lacked visible amounts of endogenous and transgenic PCR products, this indicated that the quality and/or quantity of genomic DNA did not allow the PCR product to be obtained. Assessments were not performed for these plants. It was necessary to repeat the isolation of genomic DNA and perform a new PCR run with appropriate controls. 6.8. Use of differential PCR protocol for identification of EE-C.4.M2

A test run with appropriate controls was required before attempting to screen unknown samples. The developed protocol may require optimization of components, which may differ in different laboratories (isolation of template DNA, Tad-DNA polymerase, quality of primers, aMTP, thermal cycler, etc.).

Amplification of the endogenous sequence played a key role in this protocol. It was

It is necessary to achieve PCR and thermocycling conditions during which amplification of equimolar amounts of both endogenous and transgenic sequences of known transgenic matrix genomic DNA is possible. In all cases when the amplification of the endogenous target fragment did not occur or when the amplification of the target sequences did not occur, according to electrophoresis in agarose gel with the same intensity of ethidium bromide staining, optimization of the PCR conditions could be required.

According to the protocol described above, the leaf material of a number of Siusipe tah plants, some of which contained EE-ZM2, was tested. Samples of elite event EE-ZM2 and SiIusipe tah wild type were used as positive and negative controls, respectively.

Figure 6 shows the result obtained when using the PCR amplification protocol of the elite event EE-SMA2 for a number of samples of soybean plants (lanes 1-14). The samples in lane 1 were found to contain an elite event, as a band corresponding to 185 bp was detected, while the samples in lanes 2, 3 and 4 did not contain EE-OM2. Lane 2 contained another soybean elite event, lane C was a non-transgenic Siusipe tah control; lane 4 was a negative control sample (water), and lane 5 was a molecular weight marker (ladder 100 p.o.). 7. Using a specific integrated fragment as a probe to detect material that included EE-CM2.

The specific integrated fragment EE-3M2 was obtained by PCR amplification using specific primers 5009 (ZEO IO Mo 18) and 5OM010 (ZEO IO Mo 19) or by chemical synthesis, and labeled. This integrated fragment was used as a specific probe for the detection of EE-OMA in biological samples. Nucleic acid was isolated from samples according to standard procedures.

This nucleic acid was then contacted with a specific probe under hybridization conditions optimized for hybrid formation. Detection of hybrid formation indicated the presence of EE-OM2 nucleic acid in the sample. Optionally, the nucleic acid in the samples was amplified before contact with a specific probe using specific primers. As an alternative, instead of the integrated fragment, the nucleic acid was labeled before contact with a specific probe. Optionally, the specific probe was attached to a solid support (eg, but not limited to, a filter, strip, or pellet) prior to contact with the samples. 8. Production of soybean plants, including elite events EE-5MZ3 and EE-Z3MI or EE-SM3Z and EE-

SM, and assessment of agronomic productivity of such plants.

A soybean plant containing a combination of elite events EE-ZMZ3 and EE-SOMI1 was obtained by conventional crossing of a soybean parent plant containing EE-SMZ and a soybean parent plant containing EE-CM1. Progeny plants containing both events were identified using the EE-ZMI1 and EE-CM3 PCR identification protocols as described here. In particular, plants containing EE-SOMZ and several EE-CM1 lines were crossed. The resulting E1 plants were grown and sprayed with glyphosate and glufosinate. E2 seeds were collected from E1 plants and planted (E2 plants were not sprayed). Single E2 plants were destroyed. Rows with E2:EZ3 plants were grown and sprayed with glyphosate and glufosinate. Rows homozygous for both EE-ZM3 and EE-SMI1 were identified and subsequently harvested from them. Cleavage data from these trials showed the expected Mendelian cleavage events.

A soybean plant containing a combination of elite events EE-ZMZ3 and EE-SOM2 was obtained by traditional crossing of a soybean parent plant containing EE-SMUZ and a soybean parent plant containing EE-CM2. Progeny plants containing both events were identified using the EE-ZM2 and EE-CM3 PCR identification protocols as described here. In particular, plants containing EE-OM3Z and plants containing EE-65M2 were crossed. The obtained E1 plants were crossed with 4 traditional lines. E1 plants resulting from these crosses were grown in the field and sprayed with glyphosate and glufosinate. PE2 seeds collected from E1 plants resistant to both herbicides were subsequently planted. E2 plants were sprayed with both glyphosate and glufosinate.

Nine hundred plants resistant to both herbicides were collected and planted during field trials. In this test, we obtained data corresponding to the expected Mendelian splitting. For agronomic uniformity, approximately 40 lines homozygous for resistance to both herbicides were selected in further studies. These lines had good resistance to both herbicides and good agronomic characteristics.

Field trials of such soybean plants containing combined events in different types of commercial germplasm were performed in different areas, evaluating different agronomic

Plant parameters including plant height, height to node, lodging tolerance, growth, yield and level of resistance to glyphosate, isoxaflutol and glufosinate. 9. Introgression of EE-CM3Z and EE-OMI1 or EE-OM2 into better varieties.

Elite event EE-SOMZ and elite event EE-OMI1 and EE-60M2 by backcrossing were included in commercial soybean varieties, for example, but not limited to, soybean variety 7631014 (052009252860); soybean variety 7431014 (052009252859); soybean variety 7925084 (052009252858); soybean variety 7311153 (052009252857); soybean variety 5070159 (052009252856); soybean variety 7535357 (052009246353); soybean variety 5070160 (0052009246352); soybean variety 26074414 (052009249508); soybean variety 7509171 (052009249507); soybean variety 5ХО70158 (52009246351); soybean variety 7511119 (252009249506); soybean variety 7113111 (052009238945); soybean variety 506-022M018047 (057592518); soybean variety 7013345 (052009232957); soybean variety 7041461 (052009235376); soybean variety 7549450 (052009232956); soybean variety 7317090 (052009232955); soybean variety 2M2Uu58015 (052009226597); soybean variety 7243182 (052009226596); soybean variety 7143182 (052009226595); soybean variety 7043182 (052009220673); soybean variety 5070157 (052009222950); soybean variety 306924721 (052009220672); soybean variety 7614385 (052009220671); soybean variety 7925118 (052009214750); soybean variety 7821295 (052009214749); soybean variety 7811336 (052009214748); soybean variety 5070150 (052009214747); soybean variety 6214260 (052009214746); soybean variety 5070152 (0Ш52009214745); soybean variety 7429331 (0052009214751); soybean variety 26034631 (052009208634); soybean variety 507-039UK108674 (57560621); soybean variety 5O7-OZKI 016279 (057560620); soybean variety 506-SI 959411 (057554017); soybean variety 55387OMKK (052009158453); soybean variety NERK-S (СА2645702); soybean variety 506-029)K423016 (057521606); soybean variety 506-019U8119814 (057518039); soybean variety 506-019UK119448 (057518038); soybean variety 6540220 (052009055960); soybean variety 5060292 (052009055959); soybean variety 5050228 (052009055958); soybean variety 506-029K423003 (057491873); soybean variety 506-029)K423005 (057491872); soybean variety 506-029K409114 (057485782); soybean variety 506-59144056 (057473823); soybean variety (57470835); soybean variety 6910450 (052008282369); soybean variety 6223012 (57446246); soybean variety 6549250 (57446245); soybean variety 17731225 (052008271204); soybean variety 6928285 (052008271203); soybean variety 6736054 (052008271169); soybean variety 5060299 (052008271199); soybean variety 5060294 (052008271202); soybean variety 6943322 (052008271201); soybean variety 5343260 (Ш52008263719); soybean variety 6439359 (052008263704); soybean variety 6238359 (52008263703); soybean variety 6547272 (052008263702); soybean variety 6929431 (052008263701); soybean variety 6703392 (052008263700); soybean variety 6044483 (052008263699); soybean variety 5050224 (052008263698); soybean variety 60 6719022 (052008263697); soybean variety 5826056 (052008263696); soybean variety 6265047 (052008263724);

soybean variety 6928331 (052008263695); soybean variety 6719331 (052008263694); soybean variety 6636454 (052008263693); soybean variety 6226454 (052008263718); soybean variety 00073801 (052008256657); soybean variety 6326393 (052008256652); soybean variety 6408448 (I052008256651); soybean variety 6449315 (052008250524); soybean variety 5060296 (052008250523); soybean variety 6012078 (052008250522); soybean variety

B 6342078 (52008250521); soybean variety 6419156 (52008250520); soybean variety 5723264 (y52008250519); soybean variety 5050225 (052008250518); soybean variety 5060298 (052008244783); soybean variety 6935331 (052008244782); soybean variety 6819456 (52008244787); soybean variety 5060297 (52008244781); soybean variety 6135319 (52008244786); soybean variety 6819331 (52008244780); soybean variety 6137445 (52008244779); soybean variety 6917445 (052008244778); soybean variety 6111333 (052008244777); soybean variety 5050229 (052008244776); soybean variety 6114011 (052008244775); soybean variety 6900358 (52008244784); soybean variety 6345184 (052008244774); soybean variety 6836085 (y52008244773); soybean variety 6635047 (I52008244772); soybean variety 6139105 (052008244771); soybean variety 6434385 (052008244770); soybean variety 5060295 (052008244769); soybean variety 6035184 (52008244768); soybean variety 5060293 (52008209590); soybean variety 6733322 (052008209594); soybean variety 6421326 (y52008209593); soybean variety 5060077 (052008209589); soybean variety 6600375 (052008209592); soybean variety 506-C1821457 (057420104); soybean variety 507- 02Ko294306 (57414178); soybean variety 506-BA046119 (057414175); soybean variety 507-02K0294307 (057411114); soybean variety 503865M (052008189802); soybean variety 6701475 (57408097); soybean variety 1335025 (052008178316); soybean variety 1686017 (052008178315); soybean variety 2388028 (y52008178314); soybean variety 2387029 (052008178313); soybean variety 506-LLLY52330 (57388129); soybean variety 6424090 (57385118); soybean variety 6723322 (57385115); soybean variety 504377MEV. (57385114); soybean variety 506- 02u8111334 (57381864); soybean variety 6141287 (57378577); soybean variety MT110501 (057378576); soybean variety (057378575); soybean variety 506-MLL169267 (57375261); soybean variety 6223392 (57371939); soybean variety 506-C1 968413 (057371937); soybean variety 506-С1951107 (57368636); soybean variety 506-MT9152077 (057361810); soybean variety 4211676 (y52008092253); soybean variety 506-MO59029 (57355101); soybean variety 8548193 (57345228); soybean variety 6440261 (57345227); soybean variety 5060291 (57342151); soybean variety 506-MT9206166 (057339094); soybean variety 506-M/M/013107 (57339093); soybean variety 506-MOZ3256 (57335820); soybean variety 6134466 (57332656); soybean variety 506-01U8123373 (57329800); soybean variety 506-MT9211059 (57326831); soybean variety 26170838 (052008016590); soybean variety 306612412 (052008016588); soybean variety 26660135 (52008016587); soybean variety 306734323 (52008016586); soybean variety 506-019U8122235 (57317144); soybean variety 5900450 (057314986); soybean variety 506-MT116260 (057314984); soybean variety 506-54143606 (57312381); soybean variety 506-98181-201-35167 (57309819); soybean variety 26082635 (057304219); soybean variety BA922834 (57304217); soybean variety 0198123480 (057304216); soybean variety MO61422 (057304215); soybean variety 17082821 (052007277262); soybean variety 17621620 (052007277261); soybean variety 00977706 (052007277260); soybean variety 5060182 (052007277259); soybean variety 26312034 (57301078); soybean variety 26143837 (057301077); soybean variety 435.7C5 (052007271626); soybean variety 495.8 (052007271625); soybean variety 5306230 (57297845); soybean variety 506-MLL/167686 (057291772); soybean variety 6141145 (052007245426); soybean variety 5050232 (052007226829); soybean variety 5333301 (y52007226828); soybean variety 5050215 (52007226827); soybean variety 3235020 (052007226826); soybean variety 5720482 (052007226825); soybean variety 5050216 (52007226824); soybean variety 5512112 (052007226823); soybean variety 3233021 (052007226822); soybean variety 1336024 (052007226821); soybean variety 5348287 (y52007226820); soybean variety 5204220 (y52007226819); soybean variety 6188027 (052007226818); soybean variety 4183026 (052007226817); soybean variety 506-M/M/157958 (057271325); soybean variety 5733056 (052007209091); soybean variety 90501911 (052007209090); soybean variety 5050221 (y52007204361); soybean variety 5802205 (052007204360); soybean variety 1000642 (52007204359); soybean variety 5420128 (052007204358); soybean variety 5050222 (052007199094); soybean variety 5050217 (052007199093); soybean variety 5050223 (052007199092); soybean variety 5050218 (052007199091); soybean variety 5419227 (052007199089); soybean variety 5319227 (052007199088); soybean variety 5723045 (52007199087); soybean variety 5051007 (052007199086); soybean variety 5826175 (052007192893); soybean variety 5050231 (052007192892); soybean variety 5826376 (y52007192891); soybean variety 5628386 (052007192890); soybean variety

BO 5138236 (052007186307); soybean variety 5608398 (52007186306); soybean variety 5050230 (52007 186305); soybean variety 5624126 (052007180561); soybean variety 5019225 (052007180560); soybean variety 5549483 (052007180559); soybean variety 4189010 (y52007180551); soybean variety 1486018 (I52007180550); soybean variety 5050235 (052007180549); soybean variety 5023230 (I52007180548); soybean variety 5050238 (y52007174930); soybean variety 5830261 (052007174928); soybean variety 5050226 (57247772); soybean variety 5806063 5B (05724771); soybean variety 5050233 (57244881); soybean variety 5726085 (57241939); soybean variety MTO00792 (57238867); soybean variety 5521161 (057235718); soybean variety M/LL109447 (57235717); soybean variety

VAZA7474 (57220898); soybean variety 5939002 (57217870); soybean variety 5726175 (57217869); soybean variety 5432082 (57217868); soybean variety 50085028. (57211715); soybean variety 501750 MEV. (57208658); soybean variety MTO17827 (57208657); soybean variety AM2M74028 (57205458); soybean variety SI 431203 (57202400); 60 soybean variety AMO563222 (57199288); soybean variety 5520279 (57196253); soybean variety 5834401 (57196252);

soybean variety 5621161 (57196251); soybean variety SI 722114 (57196250); soybean variety 5741081 (57193140); soybean variety SI727422 (057186895); soybean variety 4M2U55022 (57183468); soybean variety 5083011 (057173169); soybean variety 5626085 (57169976); soybean variety 5050051 (57169974); soybean variety 4506816 (052006294626); soybean variety MLL/152201 (57132594); soybean variety SI 727636 (57132593); soybean variety

B MO8851 (57126047); soybean variety 4324401 (57105728); soybean variety 5050164 (57105727); soybean variety 4136015 (052006195931); soybean variety 3133014 (052006195930); soybean variety 5040132 (52006195929); soybean variety 4328386 (052006195928); soybean variety 1339013 (052006195927); soybean variety 4423183 (052006195925); soybean variety 5040131 (052006195924); soybean variety 4929388 (52006195923); soybean variety 4817034 (052006195922); soybean variety 4916816 (57098385); soybean variety 4713487 (52006191032); soybean variety 4348019 (052006191031); soybean variety 5040122 (052006191030); soybean variety 5040133 (052006185031); soybean variety SI 821418 (057091404); soybean variety 4441080 (057091403); soybean variety 4805442 (052006179509); soybean variety 4921237 (052006179508); soybean variety 4417380 (52006174369); soybean variety 4405070 (052006174368); soybean variety 4417779 (57084328); soybean variety 5040125 (052006168678); soybean variety 4909380 (57081572); soybean variety 5050162 (057081571); soybean variety 86084016 (57081570); soybean variety 5050163 (57078600); soybean variety 5040135 (57078598); soybean variety 5040117 (57078597); soybean variety MO3393 (57071391); soybean variety 4145306 (57064253); soybean variety 900213 (052006117405); soybean variety 1000126 (052006117404); soybean variety 901023 (052006117403); soybean variety 5040130 (57053280); soybean variety 4706198 (57053279); soybean variety 5040118 (57053278); soybean variety 5040119 (57053277); soybean variety 5040123 (57053276); soybean variety 4442112 (57049497); soybean variety 917013 (57045689); soybean variety 5040124 (57045691); soybean variety 4238491 (57045690); soybean variety 5010136 (57041882); soybean variety 900613 (057030297); soybean variety 4337175 (57030301); soybean variety 5040121 (57030300); soybean variety 4216033 (57030299); soybean variety 5040128 (57022901); soybean variety 5040120 (57022900); soybean variety 5040127 (57019199); soybean variety 5040134 (57015378); soybean variety 5040129 (57015377); soybean variety 4513420 (57005564); soybean variety 943013 (52006031958); soybean variety 5030136 (052006021081); soybean variety 927013 (052006021080); soybean variety 1000109 (052006015962); soybean variety 90046112 (052006010530); soybean variety 90897327 (052006010529); soybean variety 90362421 (052006010528); soybean variety 03022253 (052006010527); soybean variety 02022433 (052006010526); soybean variety 02022323 (I52006010525); soybean variety 02912951 (y52006010524); soybean variety 0102115 (052006010523); soybean variety 915034 (052006010522); soybean variety 0509255 (052006010521); soybean variety 4803070 (056982368); soybean variety 4704310 (056979762); soybean variety 54919784 (052005268362); soybean variety SI 615261 (52005268361); new soybean variety (0520041999654); soybean variety 0509214 (052005210542); soybean variety 70826751 (052005193442); soybean variety 0509243 (052005193441); soybean variety 0509246 (y52005193440); soybean variety 0509253 (052005193439); soybean variety 0509247 (052005193438); soybean variety 0509252 (052005193437); soybean variety 0509241 (y52005193436);

ZB soybean variety 0509249 (56884927); soybean variety 0509244 (052005183158); soybean variety 0509240 (052005183157); soybean variety 0509239 (y52005183156); soybean variety 0509254 (052005183155); soybean variety 0509245 (052005183154); soybean variety 0509251 (052005183153); soybean variety 4283008 (56888050); soybean variety 2386009 (052005183152); soybean variety 3282002 (56870080); soybean variety 0509248 (052005183151); soybean variety 5091007 (56906249); soybean variety 0509236 (052005166281); soybean variety 0509235 (052005166280); soybean variety 0509237 (052005166279); soybean variety 505322MEV (052005164390); soybean variety ХО5030МЕВ. (y52005166278); soybean variety 504911MRE. (052005166277); soybean variety 5030153 (052005160504); soybean variety 5030158 (052005144680); soybean variety 5030160 (052005144679); soybean variety 5030161 (052005144678); soybean variety 5030157 (52005144677); soybean variety 5030155 (y52005144676); soybean variety 5030156 (052005144675); soybean variety 5030159 (y52005144674); soybean variety 5030154 (056900376); soybean variety 5020030 (052005114929); soybean variety 5030010 (052005114928); soybean variety 50143128. (052005097629); soybean variety 501330 MEV. (I52005097642); soybean variety 5030150 (052005071900); soybean variety 5022209 (052005050601); soybean variety 5022217 (052005050600); soybean variety 5022219 (052005050599); soybean variety 5030151 (052005050598); soybean variety 0491735 (Ц52005022272); soybean variety 5022218 (y52005022271); soybean variety 6190006 (иЙ52004268447);

BO soybean variety 50112028. (052004250316); soybean variety 0487681 (052004237153); soybean variety 0491717 (052004237152); soybean variety 5022220 (052004237151); soybean variety 0491715 (I52004237150); soybean variety 0491712 (052004237149); soybean variety 0491718 (052004237148); soybean variety 99271316 (052004221344); soybean variety 5022212 (052004221343); soybean variety 0491737 (I52004221342); soybean variety 5022211 (0Й52004221341); soybean variety 5022210 (052004221340); soybean variety 5022213 5B 0 (052004221339); soybean variety 0491725 (052004221346); soybean variety 03129016 (052004221329); soybean variety 5022208 (052004221328); soybean variety 5022207 (052004221345); soybean variety 02932 (I52004210968); soybean variety 94137321 (052004205862); soybean variety 94106224 (052004205861); soybean variety 94143901 (052004205859); soybean variety 0487685 (52004205858); soybean variety 92440927 (052004205857); soybean variety 0487686 (y52004205856); soybean variety 5022215 (052004205855); soybean variety 5022214 (052004205854); 60 soybean variety 0491726 (052004205849); soybean variety 92478609 (052004205853); soybean variety 922013

(056781040); soybean variety 0491727 (052004205852); soybean variety 0491728 (52004205851); soybean variety 1465003 (052004098766); soybean variety 3186004 (052004019936); soybean variety 7085005 (52004205850); soybean variety 5022204 (052004199958); soybean variety 5022206 (052004199957); soybean variety 0491731 (052004199956); soybean variety 0491733 (I52004199955); soybean variety 0491738 (I52004199954); soybean variety

B 0491732 (52004199953); soybean variety 0491729 (52004199952); soybean variety 5020011 (052004199951); soybean variety 0491739 (052004199950); soybean variety 0491730 (052004199949); soybean variety 13873 (052004199948); soybean variety 954011 (052004181822); soybean variety 010022 (I52004181831); soybean variety 4181001 (052003229926); soybean variety 0491721 (052004168228); soybean variety 0491723 (56911581); soybean variety 0491716 (052004168226); soybean variety 0491713 (052004168225); soybean variety 0491711 (052004168224); soybean variety 0491722 (052004168223); soybean variety 0491724 (052004168222); soybean variety 0491720 (y52004168221); soybean variety 0487682 (52004168220); soybean variety 0491714 (52004168219); soybean variety 0491719 (052004168218); soybean variety OR 5634 BE. (y52003177540); soybean variety 556-07 (052004098765); soybean variety 926877 (052004064859); soybean variety ZE73753 (52004055059); soybean variety 5M83594 (052004055058); soybean variety 5E71112 (052004055056); soybean variety 5E73090 (052004055054); soybean variety 5M79526 (052004055053); soybean variety 5MM90702 (052004055052); soybean variety 5M79525 (052004055051); soybean variety 5E90345 (052004055050); soybean variety 0149928 (052004055049); soybean variety 5M83780 (052004055048); soybean variety 0053840 (I52004055047); soybean variety 924001 (y52004055046); soybean variety 0004747 (052004055057); soybean variety 0037357 (y52004055045); soybean variety 5M83366 (052004055044); soybean variety 5M76208 (052004055043); soybean variety 0037370 (052004055042); soybean variety 5X95512 (052004049821); soybean variety 0096008 (y52004049820); soybean variety 5M83544 (052004049819); soybean variety 0088401 (052004049818); soybean variety 5M64195 (052004049817); soybean variety 0034754 (52004049816); soybean variety 5M71173 (52004049815); soybean variety 5M83211 (052004049814); soybean variety 92422749 (052004045058); soybean variety 0120311 (052004045057); soybean variety 5010344 (y52004003438); soybean variety 70876922 (y52004003437); soybean variety 924496 (052004003436); soybean variety 19705120 (052003237116); soybean variety 19704220 (052003235914); soybean variety 19704280 (052003237115); soybean variety 19704210 (052003237114); soybean variety 537-M4 (052003237113); soybean variety 19602310 (052003233685); soybean variety 19704120 (052003233684); soybean variety 19704230 (052003233683); soybean variety 1000126 (052003233682); soybean variety 93831526 (052003221226); soybean variety 0322581 (052003221225); soybean variety 0332149 (052003213028); soybean variety 0332144 (052003213027); soybean variety 924788 (52003213026); soybean variety 924861 (052003213025); soybean variety 928070 (052003213024); soybean variety 5010354 (052003213023); soybean variety 5010360 (052003213022); soybean variety 5010361 (052003213021); soybean variety 5010363 (052003213020); soybean variety 5010364 (052003213019); soybean variety 0332148 (052003208805); soybean variety 0332147 (052003208804); soybean variety 0332146 (52003208803); soybean variety 0332135 (I52003208802); soybean variety 1000144 (052003208801); soybean variety 0332143 (052003208800); soybean variety 0332145 (052003208799); soybean variety 5010345 (052003204884); soybean variety 0332131 (52003204883); soybean variety 0332130 (y52003204882); soybean variety 0332129 (52003204881); soybean variety 0332122 (y52003204880); soybean variety 5010350 (052003204879); soybean variety 5010355 (052003204878); soybean variety 031766 (052003204877); soybean variety 5010353 (052003204876); soybean variety 0322580 (I52003200579); soybean variety 400 0322579 (y52003200578); soybean variety 5010347 (052003200577); soybean variety 5010349 (52003200576); soybean variety 0332141 (052003200575); soybean variety 0332142 (052003200574); soybean variety 0332133 (052003200573); soybean variety 0332134 (52003200572); soybean variety 0332139 (y52003200571); soybean variety 0332137 (052003200570); variety of soybean ХВ33008 (57598435); variety of soybean KhV27008 (057592519); variety of soybean ХВ41МО8 (57589261); soybean type KhVO5U08 (57589260); variety of soybean ХВ33ТО8 (57589259); variety of soybean ХВ3ЗОЖХО8 (57586025); variety of soybean KhVA0Ш08 (057582813); variety of soybean ХВ29МО8 (57582811); variety of soybean 93710 (052009144843); variety of soybeans 04325666. (y52009055957); variety of soybeans 04125897 (052009055956); variety of soybeans 04698573 (052009055955); variety of soybean 04356652 (052009019592); soybean type 04456885 (052009019591); variety of soybeans 04698013 (052009019590); type of soybean 04637114

BO (052009019589); variety of soybeans 04102367 (052009019595); variety of soybean 04266582 (052009019594); variety of soybeans 04422801 (052009019593); variety of soybeans 04520980 (052009019588); variety of soybean 04521369 (I52009019587); variety of soybean 04223057 (052009019586); variety of soybean 04682156 (I52009019585); variety of soybean 04233569 (052009019584); variety of soybeans 04925614 (052009019583); variety of soybean 04203144 5B (052009019604); variety of soybean 04102536 (052009019582); variety of soybeans 04865324 (052009019581); variety of soybeans 04825495 (052009019580); variety of soybeans 04659251 (052009019579); variety of soybean 04258962 (I52009019578); variety of soybeans 04253969 (052009019577); variety of soybeans 04696658 (052009019603); variety of soybeans 04256925 (052009019576); variety of soybean 04253681 (052009019575); variety of soybeans 04789254 60 (052009019574); variety of soybean 04713125 (052009019573); type of soybean 04526223

(052009019572); variety of soybean 04556201 (I52009019571); variety of soybeans 04012368 (052009019570); variety of soybean 04452019 (052009019569); variety of soybean 04201483 (052009019568); variety of soybean 04463892 (I52009019567); variety of soybeans 04159630 (052009019566); variety of soybean 04470236 (052009019565); variety of soybeans 04063284

B (082009019564); variety of soybean 04021792 (052009013429); variety of soybeans 04902530 (052009013428); variety of soybean 04367012 (0Й52009013427); variety of soybeans 04923560 (052009013426); variety of soybeans 04253854 (052009013425); variety of soybeans 04210110 (052009007290); variety of soybean 04523081 (052009007289); variety of soybeans 04328762 (052009007288); variety of soybean 04483789 (I52009007287); variety of soybean 04311702 (052009007286); variety of soybeans 04127789 (052008313765); variety of soybeans 04361423 (052008313764); variety of soybean 04208814 (052008313763); variety of soybean 04201139 (052008313762); variety of soybean 04120384 (052008313761); variety of soybean 04572906 (052008313760); variety of soybeans 04301279 (052008313759); variety of soybeans 04422957 (052008313758); variety of soybeans 04256958 (52008313757); variety of soybean 4074328 (y52008282366); variety of soybean KhVA7O06 (052008244767); variety of soybean ХВ26ВО6 (052008244766); variety of soybean 4991629 (I52008216190); variety of soybean 4158090 (052008216189); variety of soybean ХВ4А0КО7 (052008209582); variety of soybeans 00069201 (052008178345); variety of soybeans 00064801 (052008178320); variety of soybean 00063801 (052008178344); variety of soybeans 00061501 (052008178343); variety of soybean 4938051 (052008178319); variety of soybean 4880500 (052008178318); variety of soybean 4835953 (052008178317); variety of soybean 4684181 (052008178342); variety of soybean 4427363 (052008178340); variety of soybean 4676311 (052008178339); variety of soybean 4953710 (052008178337); variety of soybean 4857548 (052008178336); variety of soybean 4551757 (052008178335); variety of soybean 4027271 (052008178334); variety of soybean 4274171 (052008178333); variety of soybean 0341931 (052008178332); variety of soybean 4282159 (052008178331); variety of soybean 4852004 (052008178330); variety of soybean 4688589 (052008178329); variety of soybean 4614131 (052008178327); variety of soybean 4201823 (052008178326); variety of soybean 92M22 (052008178350); variety of soybean 4174206 (052008178322); variety of soybean 4305498 (052008178321); variety of soybean 4423586 (052008172761); variety of soybean 4568207 (052008172756); variety of soybean 4840308 (052008172755); variety of soybean 4256323 (I52008172754); variety of soybean 4789516 (57399907); variety of soybean 90740 (052008168581); variety of soybean 4959932 (57396983); variety of soybean 4062885 (057394000); variety of soybean 4858197 (57390940); variety of soybean 4092390 (57390939); variety of soybean 4735486 (057390938); variety of soybean 4219527 (57388132); variety of soybean 4599695 (57388131); variety of soybean 4554257 (57388130); variety of soybean 4896902 (57385113); variety of soybean 4367308 (57385112); variety of soybean 4589609 (057385111); variety of soybean 4640250 (57385110); variety of soybean 4540394 (57385109); variety of soybean 4297661 (57385108); variety of soybean 4958786 (57381866); variety of soybean 4440685 (57375262); variety of soybean 4008211 (57371938); variety of soybean 4778469 (57368637); variety of soybean 4766295 (57355103); variety of soybean 4436909 (057355102); variety of soybean 4812469 (57351886); variety of soybean 4761767 (57351885); variety of soybean 4142393 (57329801); variety of soybean 4502135 (57326832); variety of soybean 4353363 (57321082); variety of soybean 91842 (57317143); variety of soybean 0330739 (052007271622); variety of soybean 0384279 (57294768); variety of soybean 4175567 (052007256187); variety of soybean 4336643 (52007256186); variety of soybean 4671685 (052007256185); variety of soybean 4309194 (052007256190); variety of soybean 0330738 (052007256184); variety of soybean 0045477 (052007256183); variety of soybean 0437973 (052007256182); variety of soybean 0457028 (052007256181); variety of soybean 0367478 (52007256180); variety of soybean 0358232 (052007256179); variety of soybean 0417158 (052007256178); variety of soybean 4559809 (052007256177); variety of soybean 0196172 (052007256176); variety of soybean 4785923 (052007256175); variety of soybean 4587513 (052007256174); variety of soybean 0409670 (52007256173); soybean variety 4010165 (52007256172); variety of soybean 0421133 (052007256171); variety of soybean 0240187 (052007256170); variety of soybean 0387907 (052007256169); soybean variety 0232405 (052007256168); variety of soybean 0146529

BO (052007256167); variety of soybean 4788561 (052007256166); variety of soybean 457114 (052007256165); variety of soybean 0149217 (052007256164); variety of soybean 4247825 (052007254366); variety of soybean 0212938 (052007256163); soybean variety 0146565 (052007256162); variety of soybean 4647672 (052007256161); variety of soybean 0215818 (52007256160); variety of soybean 0384531 (52007256159); variety of soybean 4878185 (052007254365); variety of soybean 4498438 (052007256158); a type of soybean

BB 0436052 (052007256157); variety of soybean 4782157 (052007256156); variety of soybean 0385457 (052007256155); variety of soybean 0385240 (52007256154); variety of soybean 4735316 (Ц52007256153); variety of soybean 0277524 (052007256152); variety of soybean 0276951 (052007256151); a type of soybean

KhV37107 (052007245429); variety of soybean ХВ35 x 07 (052007226837); variety of soybean ХВ35507 (052007226836); soybean variety KhV35EO7 (I52007226835); variety of soybean Khvzarot (y52007226834); 60 soybean variety KhV34107 (052007226833); variety of soybean ХВ34007 (052007226832); variety of soybean hv336507 (052007226831); variety of soybean 9811 (052007169220); variety of soybean 0137335 (057241941); variety of soybean ХВ15Е07 (052007150980); variety of soybean 92M52 (052007150979); variety of soybean ХВ47КО7 (052007136888); variety of soybean ХВ46ЭМО7 (0052007136887); a type of soybean

KhV57EO7 (052007136886); variety of soybean ХВ54 x 07 (052007136885); variety of soybean ХВ54М07 (052007136884); variety of soybean KhV52007 (052007136883); variety of soybean ХВ37МО07 (052007136882); variety of soybean ХВ379У07 (052007136881); variety of soybean ХВ34007 (052007136880); variety of soybean ХВ32507 (052007136879); variety of soybean ХВ329У07 (052007136878); variety of soybean hv31K07 (052007136877); variety of soybean ХВ31907 (052007136876); variety of soybean ХВ29КО7 (052007136875); variety of soybean ХВ31НО07 (052007136874); variety of soybean ХВ30007 (052007136873); variety of soybean ХВЗОЕЕО7 (052007136872); soybean variety KhV25EO7 (I52007136871); variety of soybean ХВ26б6 х 07 (052007136870); soybean type KhV23I 07 (052007136869); soybean type хХВвВ19207 (052007136868); variety of soybean KhV19E07 (0052007136867); variety of soybean ХВ18МО7 (052007136866); variety of soybean ХВ18КО7 (052007136865); variety of soybean ХВ18ХУ07 (052007136864); variety of soybean ХВ17МУ07 (052007136863); variety of soybean KhV17007 (052007136862); variety of soybean ХВ15807 (052007136861); variety of soybean ХВ12К07 (052007136860); variety of soybean ХВ11907 (052007136859); variety of soybean ХВО4ЕО7 (052007136858); variety of soybean ХВО2КО7 (052007136857); variety of soybean KhV49U07 (052007136856); variety of soybean ХВ48 x 07 (052007136855); variety of soybean 92M75 (052007136854); variety of soybean ХВ48УМ07 (052007136853); variety of soybean KhV44007 (052007136852); variety of soybean ХВ42КО7 (052007136851); variety of soybean ХВ42НО7 (052007136850); variety of soybean KhV41U07 (052007136849); variety of soybean KhV40U07 (052007136848); a type of soybean

ХВ40 x 07 (052007136847); variety of soybean ХВ39Е0О7 (0052007136846); variety of soybean KhVZ38UMO7 (052007136845); variety of soybean ХВ38507 (052007136844); variety of soybean ХВ23МО7 (052007136843); variety of soybean ХВ31МО7 (052007130652); variety of soybean ХВ28Е0О7 (052007130651); variety of soybean hv25507 (052007130650); variety of soybean ХВ21М07 (052007130649); variety of soybean KhVO3007 (052007130648); variety of soybean KhV49007 (052007130647); variety of soybean ХхХВОб6МО7 (052007130646); variety of soybean 504-97130-15-02 (057196249); variety of soybean 504-97026-M99-42648-01 (057189896); variety of soybean 505-97016-299-21212 (57186894); variety of soybean 505-99048-19 (57164064); variety of soybean 92814 (057161065); variety of soybean 99431 (052007006350); variety of soybean 505-97177-М00-22972 (57132592); variety of soybean ХВ250506 (052006225160); variety of soybean 91M70 (052006174381); variety of soybean ХВ24КО06 (052006162029); variety of soybean 503-95368-M98-52902 (057078594); variety of soybean 505-97130-51 (057078599); soybean type KhV11I 06 (052006130187); variety of soybean 94813 (0057064251); variety of soybean 94874 (057064250); variety of soybean ХВ27906 (052006112462); variety of soybean ХВ29МОб6 (052006112460); variety of soybean ХВ28706 (052006112459); variety of soybean ХВ16УМОб6 (052006112458); variety of soybean ХВ18СО6 (052006112456); variety of soybean ХВ 10МОо6 (052006107391); variety of soybean HVObKOb (052006107390); variety of soybean KhV28U06b6 (052006107389); variety of soybean ХВО04А0б (052006107388); variety of soybean ХВ121 06 (052006107387); variety of soybean ХВО05А0б (052006107386); variety of soybean ХВ25НОб (052006107385); variety of soybean KhVZ39UMOb6 (052006107384); variety of soybean ХВ27КОб (052006107383); soybean variety KhV29KOb6 (I52006107382); variety of soybean ХВ16506 (052006107381); variety of soybean KhVZ36MOb (052006107380); variety of soybean ХВО7МОб (052006107379); variety of soybean hv2gZnNOob (I52006107378); variety of soybean ХВ35С06 (052006107377); variety of soybean ХВ321 06 (052006107376); variety of soybean ХВ58РОб (052006107375); variety of soybean KhVZ36MOb (052006107374); variety of soybean ХВ220506 (052006107373); variety of soybean ХВ36006 (052006107372); variety of soybean 91M61 (052006107371); variety of soybean ХВ32А0б6 (052006107370); variety of soybean ХВ19МО6 (052006107369); variety of soybean ХВ43СО6б (052006107368); variety of soybean hv22M06 (052006107367); variety of soybean KhVZ8EOb (052006107366); variety of soybean ХВ37006 (052006107365); variety of soybean ХВ37006 (052006107364); variety of soybean XхХВО00о06 (052006107363); variety of soybean ХВ14МО6 (052006107362); variety of soybean ХВ31НОбЭ (052006107361); variety of soybean ХВ21206 (0052006107360); variety of soybean ХВО05806 (052006107359); variety of soybean ХВ15ММ06 (052006107358); variety of soybean ХВ33МОб (052006107357); soybean variety KhV 18UU06 (052006107356); variety of soybean ХВ32МОб (052006107355); variety of soybean ХВ19РО6 (052006107354); variety of soybean 503-95021-55-138-AB (057026531); variety of soybean 94M41 (057002061); variety of soybean 91M50 (56998518); variety of soybean 92813 (56989475); variety of soybean 93868 (56989474); variety of soybean 93809 (0056979759); variety of soybean 92M00 (56972352); variety of soybean DISEASE (052005120433); variety of soybean ХВ26МО5 (052005150023); variety of soybean KhV21KO5 (I052005108795); variety of soybean ХВ28ЕО5 (052005114942); variety of soybean ХВ58КО5 (052005114941); variety of soybean hv27805 (052005114940); variety of soybean ХВ21505 (0052005150022); variety of soybean ХВ260Ш05 (052005138695); variety of soybean ХВ35КО5 (052005150021); variety of soybean ХВ18505 (052005120436); variety of soybean ХВ25С05 (0052005120435); variety of soybean 90MO1 (052005120434); variety of soybeans 60 0 xХВ22НО05 (052005150020); variety of soybean ХВ22К05 (052005114939); variety of soybean ХВ58005

(052005114938); variety of soybean KhV57005 (052005120432); variety of soybean ХВ49М05 (052005120431); variety of soybean KhV20005 (052005144683); variety of soybean ХВ418В05 (052005150019); variety of soybean ХВ38ТО5 (052005120430); variety of soybean ХВ13ТО5 (052005120429); variety of soybean xХвВ19у05 (052005120428); variety of soybean ХВ43005 (052005120427); variety of soybean ХВАОЕО5 (052005120426); variety of soybean ХВ39МО5 (052005120425); variety of soybean 99MO1 (052005120424); variety of soybean KhVZ1UMUO5 (052005223439); variety of soybean ХВ32С05 (052005114937); variety of soy hvag0ro05 (052005120423); variety of soybean 92M61 (052005120422); variety of soybean 91M91 (052005114936); variety of soybean KhV33U05 (052005120421); variety of soybean ХВ34АО5 (052005120420); variety of soybean KhV1I3005 (052005114935); variety of soybean ХВ12КО5 (0052005114934); a type of soybean

KhVZORO5 (052005120419); variety of soybean ХВ57Т05 (052005114933); variety of soybean ХВ17505 (052005114932); variety of soybean KhV25U05 (052005114930); variety of soybean ХВ25505 (052005150017); variety of soybean ХВ43УУ04 (052004177420); variety of soybean KhV44U/04 (052004177419); variety of soybean хХВ53904 (052004199960); variety of soybean ХВ43МУ04 (052004216192); variety of soybean ХВ49КО4 (052004172668); variety of soybean ХВ27РО4 (052004205864); variety of soybean KhV291I 04 (052004177418); variety of soybean ХВ29КО4 (052004177417); variety of soybean ХВ41004 (052004231017); variety of soybean hv34004 (052004177416); variety of soybean ХВО0О9904 (052004172711); variety of soybean ХВ32У04 (052004194169); variety of soybean ХВ44004 (052004172710); variety of soybean ХВ44С04 (052004172709); variety of soybean KhV10I 04 (052004172708); variety of soybean ХВ19004 (052004172707); variety of soybean hvOo2E04 (052004172706); variety of soybean ХВ25 x 04 (052004172705); variety of soybean KhV261I 04 (052004172704); variety of soybean ХВ11Р04 (052004172703); variety of soybean ХВ40204 (052004177415); variety of soybean KhV40u04 (052004181833); variety of soybean ХВО07С04 (052004181832); variety of soybean 96M20 (052004172702); variety of soybean ХВ39904 (052004172701); variety of soybean ХВ29А04 (052004172700); variety of soybean ХВ3З5РО4 (052004172699); variety of soybean ХВ58204 (052004177414); variety of soybean ХВ43КО4 (052004172698); soybean type KhV35I 04 (052004172697); variety of soybean KhvOobnOi4 (052004172696); variety of soybean ХВ59004 (052004172695); variety of soybean ХВб4С04 (052004172694); variety of soybean 95M80 (052004172693); variety of soybean ХВ35О04 (05200417 7413); variety of soybean KhVO4004 (052004177412); variety of soybean KhVO8I 04 (052004177411); variety of soybean hv1i8004 (052004177410); variety of soybean KhVI6004 (052004177409); variety of soybean ХВ55КО4 (052004172692); variety of soybean ХВ57МО4 (052004172691); variety of soybean KhV251I 04 (052004205863);

From soybean variety KhV487104 (0052004194168); variety of soybean ХВ42 x 04 (052004199959); variety of soybean hv317t04 (052004177408); variety of soybean ХВ31Ш04 (052004194167); variety of soybean KhVZOEO4 (052004177407); variety of soybean ХВ31КО4 (052004177406); variety of soybean 503-95341-A98-60618 (56909033); variety of soybean 5M97-6946 (052004168227); variety of soybean 94M70 (056864408); variety of soybean 92M70 (0056797866); variety of soybean 92M71 (056858782); variety of soybean 91M40 (056828490); variety of soybean 93M80 (056849789); variety of soybean KhVZ9MOZ (56864407); variety of soybean 93M90 (56846975); variety of soybean 90M90 (056852913); variety of soybean 92M72 (056960708); variety of soybean 91Me90 (056849788); variety of soybean 92M50 (056855876); variety of soybean 92M30 (56951974); variety of soybean 99Mb6O (56797865); variety of soybean 93M40 (056791016); variety of soybean 93M41 (056835875); variety of soybean ХВ15РОЗ (056797864); variety of soybean ХВ24НОЗ (56936752); variety of soybean ХВО5АОЗ (056815585); variety of soybean 92M80 (0056849787); variety of soybean ХВ33503 (56855875); variety of soybean ХВ48РОЗ (56797863); variety of soybean ХВ29 х 03 (56806406); a type of soybean ХВО2С03 (056800795); variety of soybean ХВ29УМ03 (056858781); variety of soybean 91M10 (56958437); variety of soybean 92M10 (056916975); variety of soybean ХВ10503 (056806405); variety of soybean 92M31 (056846974); soybean type KhV380OZ (56806404); variety of soybean ХВ34МОЗ (056803508); variety of soybean Khvzoumoz (056809236); variety of soybean ХВ379У03 (056844488); variety of soybean 5E72581 (052004148665); variety of soybean ZE90076 (052004148664); variety of soybean 5082997 (052004148663); variety of soybean 0037393 (052004148662); variety of soybean 0088414 (052004148661); variety of soybean 0149926 (052004148660); variety of soybean 0037209 (052004148659); variety of soybean 93836 (56833498); variety of soybean 90874 (056812384); variety of soybean 90851 (56818809); variety of soybean 91803 (056815584); variety of soybean 95843 (56818808); variety of soybean 95842 (56815583); variety of soybean 92847 (56812383); variety of soybean 5E90346 (052004055055); variety of soybean 0007583 (052004010824); variety of soybean 0008079 (052004010823); variety of soybean 502-АРО8041-2-333-01 (052003121076); variety of soybean 502-98041-2-251-01 (052003182694); variety of soybean 502-АР98041-2- 262-02 (052003196220); variety of soybean 502-95021-55-240-BA (052003188348); variety of soybean ARA9U4A-31572 (052003061641); variety of soybean АРО8041-1-203 (052003056251); a type of soybean

ARA9U5-15294 (52003061640); variety of soybean АРО8041-4-117 (052003056250); variety of soybean 91833 (56580018); variety of soybean 93U85 (56605762); variety of soybean 92876 (56610911); variety of soybean 92838 (56605761); variety of soybean 94824 (056613967); variety of soybean 94873 (56605760); variety of soybean 93886 (056610910); variety of soybean 91812 (56583343); variety of soybean 95834 (056605759); for variety of soybean 94823 (056605758); variety of soybean 90811 (056583342); variety of soybean 91892

(56586659); variety of soybean 95896 (56605757); variety of soybean 93872 (56566589); variety of soybean 95897 (56613966); variety of soybean 92895 (56608243); variety of soybean 93847 (56583341); variety of soybean 97852 (56605756); variety of soybean 93815 (056617499); variety of soybean 94854 (56613965); variety of soybean 93867 (056573433); variety of soybean 93887 (056727410); variety of soybean 96851 (56613964); variety of soybean 92884 (56730829); variety of soybean 92812 (56605755); variety of soybean 9007 (56320105); variety of soybean 93826 (056342659); variety of soybean 96821 (56369301); variety of soybean 92863 (56326529); variety of soybean 93846 (56323402); variety of soybean 92875 (56362400); variety of soybean 93808 (056323401); variety of soybean 97862 (0056323400); variety of soybean 92837 (56323399); variety of soybean 92856 (56339186); variety of soybean 93866 (056307131); variety of soybean 92862 (56346657); variety of soybean 92836 (056369300); variety of soybean 90873 (56316700); variety of soybean 95895 (56323398); variety of soybean 93865 (056229074); variety of soybean 92824 (056284950); variety of soybean 94853 (0056235976); variety of soybean 94822 (056140557); variety of soybean 93884 (56143956); variety of soybean 95832 (056229073); variety of soybean 95853 (56147283); variety of soybean 93835 (56153816); variety of soybean 93807 (056143955); variety of soybean 92874 (56124526); variety of soybean 92835 (56166296); variety of soybean 94845 (56162968); variety of soybean 96801 (056143954); variety of soybean 93853 (056335197).

Introgression of elite events into these cultivars was found to have no significant effect on each of the desired phenotypic or agronomic characteristics of these cultivars (without loss of yield), while transgene expression corresponding to resistance to glyphosate and/or isoxaflutol or glufosinate corresponded to commercial acceptable level.

Said combinations may further be combined with one or more other commercially available soybean events, including other herbicide resistance events, such as events described in USDA Animal and Plant Health Inspection Service applications: 09-349- 01r, 09-201-01r, 09-183-01r, 09-082-01r, 09-015-01r, 06-354-01r, 06-271-01r, 06-178-O1r, 98-238-01r, 98-014-01r, 97-008-01r, 96-068-01r, 95-335-01r, 93-258-01r, (see, for example, mumum.arnpiv.izaa.dom/Bg5/poi geo.piti ) or event МОМ89788 (resistance to glyphosate), described in О5 2006-282915, event ЮР-305423-1 (high oleic acid/resistance to acetolactate synthase inhibitors), described in УМО 2008/054747, МОМ87701, described in Ш5З2009130071, event 3560.4 ...

In the following claims, unless otherwise specified, the term "plant" includes the tissues of a plant at any stage of development, as well as any cells, tissues or organs harvested from or derived from such a plant, including any seeds, leaves , stems, flowers, roots, single cells, gametes, cell cultures, tissue cultures or protoplasts, without being limited to them.

Reference seeds containing the elite event EE-CM3 were deposited as 32-AAMM-0531 at the ICMV (Hegdysop Wiliaipod, Staiib5iope Esiage, Bucksburn, Aberdeen AWUHA, Scotland) on 12 October 2009 under the ICMV accession number 41659, and their viability was confirmed. Alternative name

EE-OM3Z - event ES-072 or M5T-EO072-3.

Reference seeds containing the EE-ZMI1 elite event were deposited as 32KKMMO368 in the CSIMP (Hegdysop Wilaipod, Staiib5iope Esiage, Bucksburn, Aberdeen AVOMA, Scotland) on 12 October 2009 under the CSIMP accession number 41658, and their viability was confirmed. Alternative name

EE-OM1-1127, A2704-12 or АСЗ-СМОС5-3.

The reference seed containing the elite event EE-ZM2 was deposited as 32СОМ0688 at the CSIMP (Regdizop Villaipou, Staireiope Eviage, Bucksburn, Aberdeen AWUOMA, Scotland) on 12 October 2009 under the CSIMP accession number 41660, and their viability was confirmed. Alternative name

EE-OM2-11 55, A5547-127 or AS5-SOMOO6-4.

Reference seed containing elite event EE-ZM3Z and EE-SMI deposited as soybean 111606 in

ATCS (American Type Culture Collection, 10801, Opimegeiu Wim., Manassas, VA, 20110-2209, USA) 11 June 2010 under ATCS Accession No. PTA-11041

Reference seed containing elite event EB-ZMZ3 and EE-ZM2 deposited as soybean

О055НХ2ХЕВ at ATCC (American Type Culture Collection, 10801, Opimegeiu Wim., Manassas, VA, 20110-2209, USA) on 11 June 2010 under ATCC Accession No. PTA-11042

The above description of the invention should be considered illustrative and not restrictive.

Those skilled in the art may make various changes or modifications to the described embodiments. These modifications can be made without departing from the essential aspects of the invention.

LIST OF SEQUENCES

"1105" VBaugu Bisvstepsev M.M.

M. V. Tesploiodiyev BIR

MABOM of the Ttpotav division

TETTOM Heviiv sSatev

EVH MatE A1iai

VU Mi111atsh

My Omyshchek

UBVBAESNE BZ'eUEeEV

BE WESHLSKEBEZE Matgs

Naveh Ovekiv

KEKESHOO bevp-MaKo "Mai Plants so, staikoh to herbicides, TK methods of their hyIdectifikanhi" 130 55754.000194 "140" VSTuBTO OA "14Aikh OTO 11-a "iiich0" BROZOTA5e5,7. "Viz 800-31-23 "chu" 51755707 kiwi" 8009-11-93 "-"и5О" 81/367281 -551 2810-07-33 "150з 99 "170" Patent version 3.3 "РИ 1 "21iz 7555 "812з DNE "5135 artificial sequence "230 khg2g3" Fragment of the vector rBEokovo x "vvi" stvrminator "yROz" (188)... 479) -28i1" With support, which includes l' deshvranslsvana oblattv. nopadinsentase from T-DNA rEt37 Vakorassekkit syshekhasivpv - "complementary lannyyuk "fay" "im pUzna. "for" her). 1556 "2032 coding postelovnkuet z-sidroxypheniyapisuvatlasksienava strain

Radodotopav Bisotevselpev Az, modified by replacing the amino acid glucine-OZO with tryptophen - complementary chain "ХУ "п" perekdnauy peptide

"ai (1557)..51558) "vaz" tTROKr HU: the coding sequence of a tyrosine-derived transitional peptide bzamitase at position 53, which contains the sequence of ribulose sublinoleum, -biphosphate chlorooxynase oxytenase 84 mauv (maize) і neziaistiv aplyzhe (yesovyashpika!, according to the description in Beygkylos aeii, (19356) nЕ5510471 - homdlementary pandyug "E80" "221" B'OTV "Vaz" (19991.. 806951 "Ia" B'evui syslimdovnistv, po includes leading vibusu: engraving of a tyuttyuvu war according to the description in sakhzhloaeom vda Ekeei (18590) lbotchvazhh about uiizhoiodu, ba, 1590:1557x complementary lamnuye "YO "two promater kro" (2070)..13359) "9232: VpaatlaV AVVSI subsole, includes the promoter area hema of the piston NYA AkaBidorviv Yepaii1zapa, which includes internal duslication (Sparotsie ei ai:, 18587) VIape

Mokhlesiakh Visoiiotu, 8, B75-191.- complementary chain -82295 "Vaiz" promoter "Ra (3360)..1337) "yiZ" vPyatav: sequence including the promoter region of Ktets rpiston

No AkavBiyorviv Bpaiiiapa, sp "2812 intron "22" (ай7т5)..(а855) "В" ipstop VZAE: cerv of introvogen ЖI variant of postovnu NO IAIT

Akaviysorvia cBziivpa "22 "vi" transition peptide "Zav". (an... «y33z TrRoro Si Boslishovnost, which encodes, is optimized of the transversal whiteid, also mixed the sequence of the rgenide of the small sub-opinice riskhushtsvs z, z-bisphosphatexoroxylasv oxygenase iev ptauv zKkukuUrudanm; and Neziizldibi5 aploavisonyapnykI!, according to the description in the opera ebodi, biIzok) sshB5MALI "22" setiz is equal to "yy" (pai8.. (BE) "3" otarzra: the coding sequence of the active mutant gene 3 phosphatsynthase yeya mauv ikukurudvy) (sebBkchp es o ai., 1987) maz7ya4193-A "yipiz terminator " 3Zh iB5566E)..1 7258) "223 'semisopAs: a sequence including a 3Z'-untranslated site. гена спістону Ба дкарічорвів садіївтне ссБарочсе б о ві., 1587) (Спаропсе ебоаї., ЗЕ") ре ета ШИЯ цчесцчасьсьз дсадасссоу ссодоссаєс засадуссвє абоудасссяс зчссусуаве бо ссдвасесад басссасосу дсовавозая чаєчесчеєас ааодечаєста ваєкадаєна 152 сзесвадУчзсе сесосачсса ствеоцьсоса зааодасуде сзасдваєву сдесдсаваєс 8 єсседвьсєва зЕвасасаца сдасассчса спсчасавек бассссаате gosushesae TO ateesUuBESE seassdsuwa Stazagastaes zagsscheyauda sessaasrsyas azvazavsosode Zoo shvssasawvsa zechssasosa etasasavchtsa astayevesae zegsazechiv asesvasascha zo avsrasaesa saassaesusye dachasosschsa vsachzchassa absssavuav vsta sse Fe tsasasschssa azschasssaye chase sassesa dassaschaes

Езасецасяя Ссазсасаєс асузсосасс сазссасЯес спведавове заасацеасе вай кЕчаашиссс аскодссвааа сесавсоєсу сосбфбосдеу Чавес даззавсвсо по чЧацеосасов зачсбсссяав Чавцчаксявсс адсадсвазе Час содсс сЕссводазва 6 часссзбсса зсзоуатасс чсясасосас адссдчассся ссзусвсясс чудес оачає 725 вадсадссЕє счассассвс чразаєсвначся соебддсЧася садсеасоааа дедовоцесе тво атжсссссса асусуєссся ччссессаєт асозсезссує совозаассв сестсаєвоє 540 акдесестеце саєкуазесо саєсаєдаанс БесСссуавсє дссссоасасе сссудаєцчає зва бспосЯссса чсчочаєчсо чассаєдссо осочсусає гсавучесеї ччазсссацчу БО спачтабась сусеосечат ассуавдіаа сасуссссас здааусєцава саасекевся ьа2о сачазчссау сесачсачас сакосоцеса сузсачасеє СчЕЗУуссвад зсУУссчаєу ково асестзадас ссдсасоудс созассасас сосасаєссє совуусбасас дазабечави 1140 сеобачассч ачскоссвєссо Чесдавасад бедассачаї асвасоадсує чесуссзаву 1290 есесровкоя ссдусачасє сзассессасо чусесоуцсує сагасаєучає сауссазУєо БОБ ссзачеьксса чочсусячЗє чЕауусасео ресааЗссев ксасчсддчав саесасУссо 12760 сасассзасч запо адассцевнач садуавдасча сассассазу стовп чкео 1380 адузітсвцчас кавЕсксЯсс стчачодеатає азЗсдеасує Ессрудаася чсдецеевсЯ ї4аао астеЕтоцєзча ацессаєчає сссзначасо пестссачЧуд басосдацсоуе сучезасуєт 5 звЕБбсдаоча астсааваясо сассацассс авсгаавьесе сагтасауаєс счосактасає 1550 сачассосьсо суссукеЧеє часостчєсо зччссссЕчч зоуаозсочео чоасуасачоя їб20 ачуастзусаф ччвстесау сосссоозає чддадсувсчо сучедуссов сзчадоссавс 80 аксасацзсс чедссабаса сачеоодуєчаєс задабасуаса Яедбссосев сьссквакса y74th seshtbachchussya sssasasovya sahtabassassa asrsstssas schsrssoasbostsu azspchzsadaye HVO zvachssayesaet ssssessats chzasoZachavga zoyadusasovo asosavoyass yadssavasucha. ївво чесассаєцяс Бадестовоє адодасазс сздесвасовч ссостлассда чдвччачасе 3820 чаачссаєся стассостсу саазсаусоа завессвсад заваксоюск ссцссвєадлаа 1980 авчаанесчаєс тазвастчеєад савсадавяє аздвасасквУ вебасЕєдву дессосксУує зако сгЕєчсасСася Єсесоєкесу васестачау Содачаувай КЄЗасчкссо сабйзацавча 2155 зчаасччска адачтачасх сооасоачаа азчасусдааа ЄбусБсраАЄ аочсавацчас 2165 ччачазсста ссссскдадо ваксачарся свсасвсадас сеізасачеся вовсабесає 2280 сеЧчечкцсас васайзаєца спсасавдсс чсочавекчє сеЕаассчас часрсасаєх 2280 здсчассстусє зсдаседева Сдабазосас гзасвабссяє себссавовв асавосочео 3355 ссасававса часа счва цсдссавкаї сссавдвассс чуявавкада ассчЧочссає 7450 сасаєтаєкс Чааахассссс асабчусскес ассрссаваза сосасдасєь асавуєвасв забєй ассдавазач асскасаася садсчавєсба Євстдассвс ясачсацесс дагоахаває 250 дакасродува завсааєчас авссаєсєвсча сссукаєчса саасзаваєя абсасасавас 2580 спссчасгсва бСЕсЄзасста ся рсабає садсдассоЯ ссчечасадса чодевацеса сеБАО севачааєеч Ессе Бавсс сасаєуєсяс Ядссас avayes vochosavssa zasossvaka tai

ЕКсдазасе соцазлаваєсав ааскчсечеса бсзсабтаєь Ссваааєств сасаєчексю 2759 їасссЄавав ясосаєцавк сасвачекаєс вассуназвна часстасаве есацсщаєтс 28750 асгаставассє собсадсвосо раасусабає суаксастоуа азаасазеца савісабато ував овссасівс свачксассу састчасаєс зчасаєсоцова сабасавеує засос 340 скЕссуаєса бааатассво саавзтссаси авубстовбає васбассаачеє авксавсєзва з00г азкечячакяє савсаєткеЕка ассрсссовв чадаавссає ачассасває авеасасрсе зво авсачатсваа чзсавставсЕ асатазавач СсБСвазасс асассзасва азасачсоас зо савссодпсаєс sssasvavsayes vaazsasachse saschasayece svavchyesesayed vasSazavssch zi1vo svsachsuuet chsvssassas vabsvschChvsa sssosachsas avastassev Yadskssudsav. зва сеавїсссадо ассадачаєс агсссрсассе сачузддаасс сачеакавєсе збасссоадо 3300 кеасасвссою ссосуассся сздосаєчса ачдосстачадч садссяссас соесачесваЧчач зз5о зсасеспаце супсасяараєсє чеЕссцуансяя Бач Ессва зазссЕцеає усевдсрацоє 3420 счачсчацава пассачеісза часи са саскавоссс ссссовазея ачевзсаасєох зав гЕсаБяастса часове ссс ачадсавучає васСеЕсавцоє здсвачечаса сааббаваає 35 чавассассв сісзаєцнає сСтасссаеча ссрсцасвоя Ссдчедвусьс боксгяасасе зопа «чссдачідес усі соусаає barsvseoes aasueaaveE Svazassyese babosvazka zeB5o chCHEtastestas stvesavachi pseseEssKas dZsssasache sgosossavaZu chvaassvava Zo. кчЧЕсдасасс ссвессасав седаєвастє зусгасасаса авссїедсаа вЕсєсоачЕцЧає 3780 авекасазсс даазозацчєс васСасссаюе зсакусссса асассавсає сазєвозаєи Зв4о асеспасазе ассзвсасає узксутсасе зсессссаое саксавсаєа сві раауєев 3ч50 сБасаазаєє адсаєцнаасс всбЄзсаєває авабсесбев сучесасаас сєдсвассся ї55о сдасєсеска азатазавос зсачсЗазайс ссссзвасай сабзасочся сзавевваве 4029 сеЕсСсдечесс свазааєвБсу сезоєрсаєь ассогвавс: зсдоасасосво асшкааавсв вОаво заазчасчаци оКБадсаасЕ збсассчссв беасачсєчцвЕ сасразтабУу чзассуєбоває 2146

EsSazchavssva gvaBasavEss zaikiuessaz zvzazvovzoass ssseeeuvesze dsstasvada yo azsvastibsas avEzhssa sesaavesssv sisssavssa vrobsetre tssawsachasa 4350 gsaayetvoes esYAZerseZzo azchuasssvad ssbvssuave sedavossob svadsozavcha 438 asacsovzae ESsChieschaesstva asseevovis 4

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Бгсазауєссо айсадоасєсса соус сс чсасвчасесє досасцсасс счассссвеє 10920 чесастастіт асчссочсода чсостсссоча чазасоссуч чсчасччача даскчсчак 10980 чаасткоадс сссрусавасєч чаздочасочо Чакчадесчас зачассавса ссвасчасааєч: 11045 суссасеадЧас адедусасса засасзасаз зоссссцває соска єсас сасачвесяз ХЕБОоЗ ссасасссце агабабссова сьсккссвоє чесасечача зупосазаса вессяввача ьо сЕрсксоЗсч чецдоЗазазе сацсассзча стсваццеся чсдавсочадт ссассавуре 31829 чаасетчвусз чучаечассс сдскгаасоаге сосдчассову чвудачаєст авдссасаде 51980 свЕсцЕБсУє зазеячсзав зеВрІЕбксазче авастаекЕЕ КусЕЄсвана свавясцаєрс 1130 ззваскчесос васачаацчеа чаасеасскаг стбусевчача сссзсссевЕ бсясабасеє 13490 счЧечЧЕсУуача аксебауаєчи соадасавнає сдасуссезе вдазсавааза савсяусваа 119650 чачсзсаксс содЕдадава часуєсчаває єдосррсаса споосавачаєсоя дасадсстає 11529

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аазаєсссстсв сакосьтоса сКсваааайс ссасувавкса сазадврсасав содавазназа 11890 жЕсСсаєзасає азсаасссає ассазсьсву свагсазства всясасаєса асасєчцава І880 аасавічасз ассахсвассз абссясакЯс асааєнавакт часЯсасвав сезесдвсст 11940 чЕсСКЄзаєся всуадстсава ссачЧчедассе чдочсчасцос ачечасзасс ассзавааато 35000 чЕссЕКЕцарЕ сграсасасод сассясазає садаусавво дадсадсаве аБЕсродчайс 13050 бсоасавадіа авассочсасс атсасаєтаї ссдаааастсЄ сСсвзевбасЕЕ Ббсассеєсава. 12199 засзсвочЯаа Есасвачета савссязаза ачасстаєва сабсадсєчайєс Кабасіває: 195189 ссуєадсаєс спаакчеаса сочасассЯз апазвсваку азаассасає чссачевсса 19540 їсвзасікасс чбаєбчасаєЄє сузбеассоча всасасавсо пасеасбасес ссбсосцасс Бе З0О асазасатса стсазарессСас авачсесугу Какясовадче бассеаскає азассячася 37360 шсзасаєсвс азвеЕсусссве спадавасса Садассасаа дазсасасеЕс сгасачакаа 15420 ачеавстіакс Гасабаачао чере адаає сасавєгазса зеавабавеса ссавосяачеса 1558 сесаевзаба сазасацавяс асасдаєвся ксваавассае вачсвазесс дсхавасоче 15550 часстсаєста сааєсобоєс ассбсоасвяса савассаєсс тоссскуцоа абсавсксач 19500 сассвзаачає сагастсасс бседачадваас Єсачечсасс сеасоассексза сюспабасссс 439669 сссгоссассої чсаддсасас аадсістада пдодассоуссяа ссосаутуча чзсасесцаз 19790 рсчсцчасяса сасссдавса абспоасксса зазаччсссаса Ббасссзсачу єсчаччачаа їа780 асакзчачеся зздсасудає асассвачеє сеоссгчавУє дазсасзчасс секчзасасся 195840 завічЧаєвсс сауачесавцяа бадсскасудс косазаєЄуає всавеєЧєваа гуаазвссаює 1290 вс розвасова сетасекека ЯсбЕсдасає чесеусавасс ссавесЯсве сбокуадчеяс 7596 саечсуачЯсваа ссасесстає сзаєсссасб сСсазавсссо ссабосаває заббассьса 13090 жсраксЧаве СебуЕЕсЕся по встаєво СЕбсЕсавач чуазастава всакссасає 13080 сесасстаса аксдаєсааст Сочбасвсає ввнассстава завесачоча сасссаєцає 1340 счазачаача сдпакасссав єпвасасссс аасассаата псавдтізсЯас вассссасав 15500 сасхтавсаса їсФаксаксає савксбсссв дкаврсвасає асавсбадусєв зсівасавадяє 13869 гацсавааає сястабавса бСваабсвеЕЕ ЕСсЧассобаа свЕсосааєсс чеоцессву 15399 вазасгайва сасубоааза сокосваяса азасцвссзодєсє чсвасессас сброссацесє їЗ580 совавасаєь десасьвсає Басостаацот сЯсяссчсса савуєзаває азаасаєдчає 13440 рекрадсдчо сахсасочсс авісасасяцча ссастдаврає заассуссза Ссзаавсача 13530 ксцасудсвк абасарсаєс Єтассчсаса сзсяувсссза сабсрсаЧес зчотвазІсса ї3550 акагсусдаєс сцдаєтоассся зазавсацаєс кессссусост Бссссабава засвабтєса 136550 сатсьвеЕско доссвависї асестртавсо уКЕсрЕсрЕес сЕСссасацдас вассзвзаттсосяї Іза сксЧасеста Задзасссаз чекрассуас ссСовассос бсадусуааєч засачуєаєш 13740 акгесоЕвсУє вассадчаєса чочасесадйо беббсссась вссббсваво Чвеессссьа ї13806 всасьакорс ссечаєсіча сссСасодаса абвзсоачіссс Фа увс ссассссвяє 15866 їсачазавсза адачссЯкес Бсраассвва ссосабсзаєс ааассооєа вчзасєзацчьс 13820 бадФтосчаєсст ассозаєчає ссавесктіса себе с сссоссссов азчесскзбає 13985 зассачабвек чЕссявувоєс чабань асстауссеє драссзазаа сгсадцуЧвевв 34040 счадчісзаєь кЕдеоссвеЕ соадегаєасе сааЕрсцахта всовасссвес бпчассваоая 4100 їи :стзазчсваЗ Єдасосссау сабсссзасе Єекксазааасє Есачсвасяа асаасчайав 14160 сессссзассЯ ассарседає сЕсЕСЯЄсай зедсдсазас ссесаєвасс безчаєсєвоє 19220 сесексауссяс дассястаце содассяссс стдсосваде савсаєсячся добссуєсса або сспссескаа чЕссаасясс чосббсоссса ссасовачаа дуссавсчас бвссссаюсо 1434 бЕсСссадсав сСУЧЕЗазача чБссавсосвз сузсаЗзосасч сосуческас здусаасяаза 14409 азе:ксуазчас чобасесовас стдссоссяе сесссасуєс чоссвсояву зєвасодесє 144650 суссазпсас сасоцточсь сстгсосацчя досьсавчєс саседссадє с суссоеечеся Щ45850 тосцесессс сіссаоваче згедосавасу ксадчевасяч саєзачавсс сязсосаєся 14580 ссачеуссуа дчатассояся сбосачесса бсавачачаєс соссцусасес чбсзацевчс 14620 соаччсссаа ядссцссрссс аассячаєсс ресбассеце сесссбоєсс дацеаччасвва БГа7о0 сачсечЕсча гавоссусся васзчечазчзя асасесвеса сасбасусзча асеседаеца 32750 ссссжадесе ссосувсува чсддасавач сбчссаазад азссосааее сеочстеся 148530

Чедтаавачьє сссаоісдач забоссавво адпоазачсцса чегесссса сдоваєсоссоа 14885 чваксуєває дссчссссЕсо асачсезвцека кЕаеєчесує бсоабудаває зозастсвсе 14340 касбсчувсодч адсасовада вссбчайочуазча зсасессаєсуч свасскчавс сссочасеча 15590 адсвассвЯдя басачаєчєс чассяссссс Єбучсесста сбвсскассє чсксасУувся сї5О6О асзесвассуд зоцчсвассу часузсзазу бсавдссувс бадотесакс зосадесвує 157199 асеЕсчацчедє спудесчася чдсбзсовосся Бодстсбсяч ссасосодад аскзаввсса 151859 зсдЧасвавсї завстссаскЕ ссецеясеусся аавкявсаєк Чачатессаса дацечехсво талЯа чЧедсчвааде вуадеаєсес: ЗасСадссоуд асзсастсса сассвазосв Чяссваанає 85300 асазвочїсссо сСзваваєцес пабсотудава зсчабоассто задсчсзадче вактжсскся 15366 сЕдЧесесдо вассасєсча чоачаскасах ссосусавад ссдесдсясс ассечесвяє 15420 апачссчассс чазассксаст увоадчкассос зуассасаци ачсчаачасс асзсочасся ач48а авчассачезчЕ вассчссасб часосассяас созадчссаєє ссодазцчаза свастсадос 15540 счасєссчасяс савзсаєсовасє аадасусика всчсечесає застосо чочакеасст ББОЮ ссбксдссЯа ЄЗ чссодаса ядсовисачадч асувоческо сбозауауєа аацчачасся 5659 вззучанччи ссчаєссяу вседазсіаа ссвазстдоч апсаєстат: чЧасчаазнусє 15790 сцувстасту сарсарсасо оссоссучача васеодзасос часододаєс дасасаваєт 15780 асуасеасаєт чассачессчася зссесоксес ведсспосвЧ поассовзачьо сосЯбсасса 15840 есесяддасос баЗдтасавс сачаачассх Ксссосасєса оссссзЗавура ссоадовоко 15900 ссчссвазчава стазуссска чаастачсаз айсссссчає ссчоезсксЯв чссесессЯячЯ ї1598О кеЕсСстСсВОсс вацсчвсеяс асестаєсвс себасезаає свсзедевса зпкачсскчео 16020 усассястсе вЧевБаєаасах айсбтасаств беБосссосу сбксаусаче сгсЯчскзаа 16090 акасавасся аагсавабеє сасесєваєса доЧеЕсСавско азавссгоцде абсаааєтсо 16145 сЧаааасгує єтсааєсства сарсосазаєа Чвапацасаа сасдаваєте дассвксоао 16209 сссзавасссс ставсаєстка сесссрдчасе ссасзабцдча соадоасавс дазазчаасасо. 16260 кЕтгсстасчес соудудаачев сссоагацава вовасдачса аадссассуа засчсодаса 16320 счастчесяса скосстасуча Саспатссва аседчасссава ЕроссскаЄЄ аачасасбава 15389 сезавсєвач стазачсдба асачсслоасо чабсасваайас сяваасавас сячсасвазчі: 15345 кову сдЧавс аасесцаєда саавессссач вассскуУусі аєковастаа вассасачес 165050 тазесусвав завкясасац аацаавачес адачсадвас авачаєсста Сабсесчясе 165650 ссавасссбаєс вагодессєгав сарсссссвза абссовгсуд десасадачая сбаагусосе 16620 ссатсоосос соксоаксває чедяаєсатса асасусдсст сазадаскує sadasadchaee 16680 zaschsaess vIstssesav aseabsevkUss koaesachases rastavsasa bchsetaesyae el

Бссазастсай сяаБаасааа абзаєтьсса заксчаксучЕ чЕвадасета СЕшесанаєт леВОоЮ чежсуссдав заааздасва бсссчачасс бЄдасввЕаьє вабдересса ссбвааєаваса 16860 азбіваавсав скссЕкссав ассссавско асессксваб ассчбчсяч Ссабссксає 16929 сгсасарсвас сасівссасе ссвечсрочЧа часесвассє дсссасаесс дадаачасст 6880 сесесаєстак апчсзаскЕЄ зсссссасза саакаєзацв ззаєсвесуку вассаасаає 47040 ваксдводає втасбсабачя чЯЕсстасаса даасізваача сабсзоєсасе ссаєтаваая. 17108 ауассасава зпчазеЕбсасск кассссвоса сзасчссасу сзвстгасбає седостсроча 1716 атЕЗкасвЕс сзсуєданса дсЧуачевсв авоктччоаде сктссвзсєвас содеуєскта то савасчсчна аччачавсаї Чччечасчче ссаєсесссє деаєтаєвсє єдесаєссде зва чодавсдаса свгдссобої сексузачав ссасассова зіссабрасає заваассдав 17340 чезссвааваєч счечеаачса госуссвовда бааксассосв абкваасясає асскозкасеа 17405 чЕчсксдває свосадясса гтааєсазса сБсссстсду СуазУсссца забессЕссва І74Б0 сгвассасаве сІЧКЕссЯЄ ваесуаєтає дЧасусвесве базасесесс бсазасадсяа 17550 Chasescheazatsa zazakchodos sEachCheasavna zast sasssteeot sakssataes 7580 pEEvayzaik ChasschasaveE Ssgoiaasyana zaasavaaava zsaksavava SEtsvsvav 17540

Tsaaasavss BogzEreESvs abdosazvsai savsebkschse zaazzzichchaz assrovatsa 17766

EVgabsaztsa ssssiaskasa dssovazat Zvaschavsus akabsvssaye ss eseass teo ssovazedava stvachaesvaa akasssavaye sbayesdachou sao 1785 "213z d "Va" Z i"z1iz DN "Vi" sshchstuchnav poserpodovnost "ai "2232 primer 5NAЙ130 "abz ssasasevsvu ЧEBSS2 "В 72 D "10 BEe dya 12" DNA "2135 ntuchna postlpidavnyfst xx "aa" primesh YMRI7Y ah 2

Edaschsaso zsgYaakchase 20

Claims (16)

FORMULA OF THE INVENTION 1. A transgenic soybean plant or a cell, part, seed or progeny thereof that contains in its genome an elite event EE-CM3Z and an elite event EE-2M2, where EE-CM3Z is foreign DNA at a specific locus, as found in the reference seed , deposited in МСИМВ under the accession number МСИМВ 41659, and EE-СМ2 is foreign DNA at a specific locus, as it is contained in the reference seed deposited in МСИМВ under the accession number МСИМВ 41660, where the specified foreign DNA in EE-СМ3Z contains the nucleotide sequence zZEO IUO MO: 20 from nucleotide 1452 to nucleotide 16638, and wherein said EE-ZMZ event also contains a 5'-flanking region immediately upstream of said foreign DNA and adjacent to said foreign DNA, and a 3'-flanking region immediately downstream of the specified foreign DNA and adjacent to the specified foreign DNA, where the specified 5'-linking region contains the nucleotide sequence ZEO IO MO: 2 from nucleotide 1 to nucleotide 1451, and the specified 3'-linking region contains the nucleo eotide sequence of ZEO IHUO MO: C from nucleotide 241 to nucleotide 1408, and where indicated foreign DNA in EE-OM2 contains, in order, the following sequences: from nucleotide 1 to nucleotide 391 nucleotide sequence of ZEO IHUO MO: 11 from nucleotide 3458 to nucleotide 3848 ; and from nucleotide 392 to nucleotide 3436 the nucleotide sequence 5E0 10 MO: 11 from nucleotide 413 to nucleotide 3457; and wherein said EE-OM2 event also comprises a 5' flanking region immediately upstream of said foreign DNA and contiguous to said foreign DNA and a 3' flanking region immediately downstream of said foreign DNA and contiguous to said foreign DNA, where the specified 5'-flanking region contains the nucleotide sequence of ZEO IJU MO: 14 from nucleotide 1 to nucleotide 311, and the specified 3'-rflanking region contains the nucleotide sequence of ZEO IJU MO: 15 from nucleotide 508 to nucleotide 1880. 2. Transgenic soybean plant or its cell, part, seed or progeny according to claim 1, the genomic DNA of which when analyzed by PCR for EE-CM3Z using two primers having the nucleotide sequences ES IU MO: 4th 5EBEO IO MO: 5 , respectively, makes it possible to obtain a DNA fragment with a length of 263 bp, and the genomic DNA of which, when analyzed by PCR for EE-OSM2 using two primers with nucleotide sequences of 56 IYUO MO: 18 and ZEO IYUO MO: 19, respectively, leads to a DNA fragment with a length of 151 bp. 3. A transgenic soybean plant or its cell, part, seed or progeny according to claim 1, where said progeny plant or progeny seed is resistant to glyphosate, glufosinate and isoxaflutol, and where said progeny plant or progeny seed contains a nucleotide sequence from nucleotide 1431 to nucleotide 1472 5EO IU MO: 2, nucleotide sequence from nucleotide 230 to nucleotide 251 BEO IU MO: 3, nucleotide sequence from 301 to nucleotide 322 5EO IU MO: 14 and nucleotide sequence from nucleotide 497 to nucleotide 518 5EO IU MO: 15. 4. Transgenic soybean plant or its cell, tissue or seed, the genome of which includes the elite event EE-OM3Z, which contains a nucleotide sequence that has at least 99 95 identity with the nucleotide sequence 5EO IU MO: 20 or with the nucleotide sequence 5EO IUO MO: 20 from nucleotide 1452 to nucleotide 16638, or its complementary chain, or includes a nucleic acid molecule that hybridizes with ZEO IU MO: 20, or with the nucleotide sequence ZEO 10 MO: 20 from nucleotide 1452 to nucleotide 16638, or its complementary chain, and the gene which includes a nucleic acid molecule that has at least 99 95 identity with the EE-OM2 nucleotide sequence or its complementary chain, or includes a nucleic acid molecule that hybridizes with the EE-ZM2 nucleotide sequence, and the reference seed containing the specified EE-CMa2 event, deposited under accession number МСИМВ 41660, in particular, where the indicated nucleotide sequence EBE-OM2 contains or represents a foreign sequence single DNA in ZEO IU MO: 14 or 15 or a sequence of foreign DNA in the plant genome between sequences 5EO IU MO: 14 and 5EO I MO: 15. 5. A method of identifying the simultaneous presence of elite events EE-ZM3 and EE-OM2 in biological samples, which includes the detection of a region specific for EE-ZM3 using a specific pair of primers or a probe that specifically recognize foreign DNA EE-СОМ3Z and 5'- a flanking region that is immediately upstream of said foreign DNA and is adjacent to said foreign DNA, or a 3'-flanking region that is immediately downstream of said foreign DNA and is contiguous to said foreign DNA, and detecting a region specific for EE-COM2 , using a specific pair of primers or a probe that specifically recognize the foreign DNA EBE-OM2 and the 5′-flanking region that is immediately upstream of the indicated foreign DNA and adjacent to the indicated foreign DNA, or the 3′-flanking region that is located immediately below for said foreign DNA and adjacent to said foreign DNA, wherein the elite event EE-CM3Z and the elite event EE-C MA are as described in claim 1. 6. The method according to claim 5, where the method includes amplification of two DNA fragments with a length between 50 and 1000 bp. from the nucleic acid present in said biological sample by means of a first polymerase chain reaction that uses at least two primers, one of which recognizes the 5'-flanking region of foreign DNA in EE-OM3 containing herbicide resistance genes, and said 5 The '-flanking region contains the nucleotide sequence of ZEO IU MO: 2 from nucleotide 1 to nucleotide 1451, or the 3'-flanking region of foreign DNA in EE-CM3 containing herbicide resistance genes, and the specified 3'-flanking region contains the nucleotide sequence of the complementary of ZEO IU MO: C from nucleotide 241 to nucleotide 1408, and the other of the specified primers recognizes the sequence within the foreign DNA EE-SM3U containing the nucleotide sequence of the complementary chain of ZEO IU MO: 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence of 5EO IU MO: From nucleotide 1 to nucleotide 240, or the second of the indicated primers recognizes the sequence within the foreign D NC EE-ZMZ3, containing the nucleotide sequence ZEO IYUO MO: 1 from nucleotide 188 to nucleotide 7252 or its complementary chain, or containing the nucleotide sequence ZEO I MO: 20 from nucleotide 1452 to nucleotide 16638 or its complementary chain, and with the help of the second polymerase chain reaction using at least two primers, one of which recognizes the 5'-flanking region of the foreign DNA in EE-CM2, which contains herbicide resistance genes, and the specified 5'-flanking region contains the nucleotide sequence 5EO IYUO MO: 14 from the nucleotide 1 to nucleotide 311, or the 3'-flanking region of foreign DNA in EE-СМа2 containing genes for resistance to herbicides, and the indicated 3'-flanking region contains the nucleotide sequence of the complementary chain 5EO IU MO: 15 from nucleotide 508 to nucleotide 1880, and the other of these primers recognizes the sequence within the foreign DNA EE-CM2, containing the 60 nucleotide sequence of ZEBEO 10 MO: 14 from nucleotide 312 to nu nucleotide 810 or the nucleotide sequence 5EO IU MO: 15 from nucleotide 1 to nucleotide 507, or the second of the indicated primers recognizes the sequence within the foreign DNA of EE-CM2 containing the nucleotide sequence ZESO IO MO: 11 from nucleotide 3458 to nucleotide 3848 and the nucleotide sequence 5EO IU MO: 11 from nucleotide 413 to nucleotide 3457 or its complementary chain, and the specified first and second polymerase reactions can be sequential or simultaneous. 7. The method according to claim 5, where the indicated primer, which recognizes the 5'-flanking region of EE-OM3, contains directly at its 3'-end a nucleotide sequence of at least 17 consecutive nucleotides, selected from the nucleotide sequence of ЗЕО ИЯ МО: 2, from the nucleotide 1 to nucleotide 1451, or the indicated primer, which recognizes the 3'-linking region of EE-OM3, contains directly at its 3'-end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complementary chain ZEO IUO MO: 3, from the nucleotide 241 to nucleotide 1408, and the indicated primer, which recognizes the sequence within the foreign DNA EE-COM3, contains directly at its 3'-end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complementary chain of ZEO IU MO: 2, from nucleotide 1452 to nucleotide 1843, or the nucleotide sequence of ZEO IU MO: 3, from nucleotide 1 to nucleotide 240, or the nucleotide sequence of 5EO IO MO: 1 from nucleotide 188 to nucleotide 7252 or its complementary chain, and the indicated primer recognizing the 5'-"flanking region of EE-CM2 contains directly at its 3'-end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of 5EO IU MO: 14, from nucleotide 1 to nucleotide 311, or the indicated primer, which recognizes the 3'-linking region of EE-CM2, contains directly at its 3'-end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence the sequence of the complementary chain ZEO IYUO MO: 15, from nucleotide 508 to nucleotide 1880, and this primer, which recognizes the sequence within the foreign DNA EE-OM2, contains directly at its 3'-end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of ZEO IU MO: 14, from nucleotide 312 to nucleotide 810, or nucleotide sequence of ZEO IU MO: 15, from nucleotide du 1 to nucleotide 507, or containing, in order, the nucleotide sequence ZEO IU MO: 11 from nucleotide 3458 to nucleotide 3848 and the nucleotide sequence 5EO IU MO: 11 from nucleotide 413 to nucleotide 3457 or its complementary chain, in particular, where the primers are indicated , specific for EE-ZM3Z, contain the sequence of ZEO IJU MO: 5 and 5EO IJU MO: 4, respectively, or the sequence of 5EO IJU MO: 7 and 5EO IJU MO: 5, respectively, the indicated primers, specific for EE-OM2, contain the sequence BEO IYU MO: 18 5EO IYUO MO: 19, respectively. 8. A kit for the identification of the simultaneous presence of the elite event EE-SMZ3Z and the elite event EE-COM2 in biological samples, which includes one primer that recognizes the 5'-rlinking region of foreign DNA in EBE-COM3, containing herbicide resistance genes, and indicated The 5'-flanking region contains the nucleotide sequence ZEO IU MO: 2 from nucleotide 1 to nucleotide 1451, or one primer that recognizes the 3'-flanking region of foreign DNA in EE-OM3Z containing herbicide resistance genes, and the 3'- the flanking region contains the nucleotide sequence of the complementary chain ZEO IUO MO: C from nucleotide 241 to nucleotide 1408, and one primer that recognizes the sequence within the foreign DNA in EE-OM3 containing herbicide resistance genes, and said foreign DNA contains the nucleotide sequence of the complementary chain 5EO IU MO: 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence of ZEO IU MO: C from nucleotide 1 to nucleotide 240, or the nucleotide sequence 5EO IU MO: 1 from nucleotide 188 to nucleotide 7252 or its complementary chain, or the nucleotide sequence of ZEO IU MO: 20 from nucleotide 1452 to nucleotide 16638 or its complementary chain, where the specified set additionally contains one primer that recognizes the 5'-flanking a region of foreign DNA in EE-OM2 containing genes for resistance to herbicides, and the specified 5'-flanking region contains the nucleotide sequence ZEO IU MO: 14 from nucleotide 1 to 311, or one primer recognizing the 3'-flanking region of foreign DNA in EBE-OM2, containing genes for resistance to herbicides, and the specified 3'-flanking region contains the nucleotide sequence of the complementary chain 5EO IYUO MO: 15 from nucleotide 508 to 1880, and one primer that recognizes the sequence within the foreign DNA in EB-OM2, containing genes for resistance to herbicides, and the foreign DNA contains the nucleotide sequence ZEBEO IU MO: 14 from nucleotide 312 to nucleotide 810 or the nucleotide sequence ZEO IU MO: 15 from n nucleotide 1 to nucleotide 507, or containing, in order, the nucleotide sequence ХЕО ИХО MO: 11 from nucleotide 3458 to nucleotide 3848 and the 60 nucleotide sequence 5EO ЙЮ MO: 11 from nucleotide 413 to nucleotide 3457 or its complementary chain, where the elite event EE -CM3Z and elite event EE-CM2 are as described in claim 1. 9. A pair of primers suitable for the specific detection of EE-CMZ and EE-OM2, in which the specified first primer contains a sequence that recognizes a sequence within the 5- or 3-flanking region of foreign DNA in EE-CM3 containing herbicide resistance genes , and the specified 5'-flanking region contains the nucleotide sequence ЭО ИХО MO: 2 from nucleotide 1 to nucleotide 1451, and the specified 3'-flanking region contains the nucleotide sequence ЭЭ 10 MO: C from nucleotide 241 to nucleotide 1408, and the specified second primer contains a sequence that recognizes a sequence within the sequence of foreign DNA adjacent to the specified 5'- or 3'-flanking region in EE-ZM3, and the specified sequence of foreign DNA adjacent to the specified 5'-flanking region contains the nucleotide sequence of the complementary chain of ZEO IO MO: 2 from nucleotide 1452 to nucleotide 1843 and the indicated sequence of foreign DNA, adjacent to the indicated 3'-flanking region, contains the nucleotide sequence 5EO IUO MO: C from nucleotide 1 to nucleotide 240, and said third primer contains a sequence that recognizes a sequence within the 5- or 3'-flanking region of foreign DNA in EE-CM2 containing herbicide resistance genes, and said 5'-flanking the region contains the nucleotide sequence 5EO IJU MO: 14 from nucleotide 1 to nucleotide 311, and said 3'-flanking region contains the nucleotide sequence cZEO IJU MO: 15 from nucleotide 508 to nucleotide 1880, and said fourth primer contains a sequence that recognizes the sequence in within the limits of the sequence of foreign DNA adjacent to the specified 5'- or 3'-flanking site in EE-oM2, and the specified sequence of foreign DNA adjacent to the specified 5'-flanking site contains the nucleotide sequence of the complementary chain ZEO IU MO: 14 from nucleotide 312 to nucleotide 810, and the indicated sequence of foreign DNA, adjacent to the indicated 3'-flanking region, contains the nucleotide sequence 5ЕО ИХО МО: 15 from nucleotide 1 to nucleotide 507, wherein the elite event EE-CM3Z and the elite event EE-CMA are as described in claim 1. 10. A pair of primers according to claim 9, where the specified first primer contains directly at its 3' end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of 5ESO IJOO MO: 2, from nucleotide 1 to nucleotide 1451, or a nucleotide sequence of size at least 17 consecutive nucleotides selected from the nucleotide sequence of the complementary chain 5EO IO MO: 3, from nucleotide 241 to nucleotide 1408, and the specified second primer contains directly at its 3'-end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complementary chain ZEO IU MO: 2, from nucleotide 1452 to nucleotide 1843, or a nucleotide sequence of at least 17 consecutive nucleotides, selected from the nucleotide sequence ZEO IU MO: C, from nucleotide 1 to nucleotide 240, where the specified third primer contains directly on its 3'- at the end a nucleotide sequence of at least 17 of consecutive nucleotides selected from the nucleotide sequence zEO IO MO: 14, from nucleotide 1 to nucleotide 311, a nucleotide sequence of at least 17 consecutive nucleotides, selected from the nucleotide sequence zEO IO MO: 15, from nucleotide 508 to nucleotide 1880, and where the fourth primer contains directly at its 3'-end, a nucleotide sequence of at least 17 consecutive nucleotides, selected from the nucleotide sequence zEO IU MO: 14, from nucleotide 312 to nucleotide 810, or the nucleotide sequence 562 IU MO: 15, from nucleotide 1 to nucleotide 507. 11. A set of primers for detecting elite events EE-SMZ and EE-OM2, as described in claim 1, which includes the following four primers: the first primer containing directly at its 3'-end the sequence ZEO IU MO: 5; the second primer containing directly at its 3'-end the sequence 5EO IU MO: 4; the third primer containing directly at its 3'-end the sequence zZEO IU MO: 18; and the fourth primer containing directly at its 3'-end the sequence FBEO IU MO: 19. 12. The method according to claim 5, where the method contains the hybridization of the nucleic acid of the biological sample with the first specific probe for EE-ZMZ3 and the second specific probe for EE-ZM2, where the sequence of the specified first specific probe is at least 80 95 identical to the sequence that contains the 5' part -flanking sequence or 3'-flanking sequence of EE-OM3Z and the sequence of foreign DNA continuous with it, and the sequence of the specified second specific probe is at least 80 95 identical to the sequence containing part of the 5'-flanking sequence or 3'-flanking sequence of EE -OMA2 and sequences of foreign DNA continuous with it, in particular, where the sequence of the specified first specific probe 60 is at least 80 95 identical to 5EO IU MO: 2 from nucleotide 1431 to nucleotide 1472 or ZEO IUO MO: From nucleotide 220 to nucleotide 261, or complementary chains of the specified sequences, and the sequence of the specified second specific probe is at least 80 95 identical to 5EO IU MO: 14 from nucleotide 301 to nucleotide 322 or 5BO IU MO: 15 from nucleotide 497 to nucleotide 518, or complementary chains of the indicated sequences. 13. A kit for identifying the simultaneous presence of an elite event EE-CM3Z and an elite event EE-CM2 in biological samples, containing a first specific probe capable of specific hybridization with a specific site of EBE-ZM3, and a second specific probe capable of specific hybridization with a specific site EE-ZM2, where the sequence of the indicated first specific probe is at least 80 95 identical to the sequence containing part of the 5'-flanking sequence or the 3'-flanking sequence of foreign DNA in EE-OM3, which contains genes for resistance to herbicides, and the sequence of foreign DNA , continuous with it, and the sequence of the specified second specific probe is at least 80 95 identical to the sequence containing part of the 5'-flanking sequence or the 3'-flanking sequence of the foreign DNA in EE-OM2 containing herbicide resistance genes and the foreign DNA sequence , continuous with it, in particular, where the sequence of the specified first specific probe contains a nucleotide residue identity, at least 80 95 identical to 5EO IUO MO: 2, from nucleotide 1441 to nucleotide 1462, or 5EO IO MO: 3, from nucleotide 230 to nucleotide 251, or complementary chains of the specified sequences, and the sequence of the specified second specific probe is at least 80 95 identical to 5EO IU MO: 14, from nucleotide 301 to nucleotide 322, or 5BO IUO MO: 15, from nucleotide 497 to nucleotide 518, or complementary chains of the indicated sequences, where the elite event EE-CM3Z and the elite event EE-OMA are as they are described in claim 1. 14. A pair of specific probes for identifying the simultaneous presence of an elite event EE-OM3Z and an elite event EE-M2 in biological samples, which includes a first probe having a nucleotide sequence at least 80 95 identical to the sequence containing part of the 5'-flanking sequence or of the 3'-flanking sequence of foreign DNA in EE-CM3 containing herbicide resistance genes and the sequence of foreign DNA continuous with it or its complementary strand, and a second probe having a nucleotide sequence at least 80 95 identical to the sequence that contains part of the 5'-flanking sequence or the 3'-Zo flanking sequence of the foreign DNA in EE-CM2 containing herbicide resistance genes and the sequence of the foreign DNA continuous with it or its complementary strand, in particular, where the sequence of the indicated first probe is at least on 80 95 identical BEO IUO MO: 2, from nucleotide 1441 to nucleotide 1462, or 5EO IO MO: 3, from nucleotide 230 to nucleotide 251, or complement entary chains of the specified sequences, and the specified second probe is at least 80 95 identical to 5EO IU MO: 14, from nucleotide 301 to nucleotide 322, or ZEO IU MO: 15, from nucleotide 497 to nucleotide 518, or to the complementary chains of the specified sequences, and where elite the EE-SMZ event and the elite EE-YAM2 event are as described in claim 1. 15. The method according to claim 5 for confirming the purity of seeds or screening for the presence of EE-CM3 in seeds, containing the detection of a site specific for EE-CM3, using a specific pair of primers or a probe that specifically recognizes foreign DNA of EE-CM3 and 5'- or a 3'-flanking sequence adjacent to said foreign DNA, and detection of a region specific for EE-COM2 using a specific pair of primers or a probe that specifically recognizes foreign EE-CMA DNA and a 5- or 3'-flanking sequence adjacent with the specified foreign DNA, in seed samples. 16. The method of detecting the presence of elite events EE-CM3Z and EE-CM2 in biological samples by hybridization with an almost complementary labeled nucleotide probe, according to which the ratio of nucleic acids probe: target is increased by repeated use of the target nucleotide sequence, which contains: a) hybridization of the specified target nucleotide sequence with the first oligonucleotide containing the nucleotide sequence ZEO IU MO: 2 from nucleotide 1452 to nucleotide 1469 or its complementary chain, or with the specified first oligonucleotide containing the nucleotide sequence 5EO IU MO: C from nucleotide 223 to nucleotide 240 or its complementary chain; B) hybridization of the specified target nucleotide sequence with the second oligonucleotide containing the nucleotide sequence ZEO IU MO: 2 from nucleotide 1434 to nucleotide 1451 or its complementary chain, or with the specified second oligonucleotide containing the nucleotide sequence 5EO IU MO: C from nucleotide 241 to nucleotide 258 or its complementary strand, wherein said first and said second oligonucleotides overlap by at least 60 per nucleotide, and said first or said second oligonucleotides are labeled, forming the indicated labeled nucleotide probe; c) cleavage of only the labeled sample as part of the double-stranded nucleotide sequence probe:target with an enzyme that causes selective cleavage of the sample, which leads to the dissociation of the double-stranded sequence and does not damage the target sequence; a) reuse of the target nucleotide sequence by repeating steps (a)-(c); and f) detection of the cleaved labeled probe and, due to this, determination of the presence of the specified target nucleotide sequence; and TU hybridization of the specified target nucleotide sequence with the third oligonucleotide containing the nucleotide sequence ZEO IYU MO: 14 from nucleotide 312 to nucleotide 329 or its complementary chain, or with the specified third oligonucleotide containing the nucleotide sequence 5EO IYUO MO: 15 from nucleotide 490 to nucleotide 507 or its complementary chain; 9) hybridization of the specified target nucleotide sequence with the fourth oligonucleotide containing the nucleotide sequence ZEO IU MO: 14 from nucleotide 294 to nucleotide 311 or its complementary chain, or with the specified fourth oligonucleotide containing the nucleotide sequence 5EO IUO MO: 15 from nucleotide 508 to nucleotide 525 or its complementary chain, where the specified third and specified fourth oligonucleotides overlap by at least one nucleotide and the specified third or specified fourth oligonucleotide is labeled, being the specified labeled nucleotide probe; P) cleavage of only the labeled sample as part of the double-stranded nucleotide sequence probe:target by an enzyme that causes selective cleavage of the sample, which leads to the dissociation of the double-stranded sequence and does not damage the target sequence; i) reuse of the target nucleotide sequence by repeating steps (T)-(Y); and ) detection of a cleaved labeled probe and, due to this, determination of the presence of the specified target nucleotide sequence, where the elite event EE-CM3Z and the elite event EE-CMaO are as described in claim 1. 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