UA110815C2 - Detection of nucleic acid sequences of the target in the analysis of the splitting and extension pto - Google Patents

Detection of nucleic acid sequences of the target in the analysis of the splitting and extension pto

Info

Publication number
UA110815C2
UA110815C2 UAA201309884A UAA201309884A UA110815C2 UA 110815 C2 UA110815 C2 UA 110815C2 UA A201309884 A UAA201309884 A UA A201309884A UA A201309884 A UAA201309884 A UA A201309884A UA 110815 C2 UA110815 C2 UA 110815C2
Authority
UA
Ukraine
Prior art keywords
nucleic acid
target
detection
pto
splitting
Prior art date
Application number
UAA201309884A
Other languages
Ukrainian (uk)
Inventor
Йонг Йун Чан
Йоунг Йо Лі
Original Assignee
Сеегене, Інк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to UAA201508100A priority Critical patent/UA115082C2/en
Priority claimed from PCT/KR2012/000287 external-priority patent/WO2012096523A2/en
Application filed by Сеегене, Інк. filed Critical Сеегене, Інк.
Publication of UA110815C2 publication Critical patent/UA110815C2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Винахід належить до способу детекції нуклеїновокислотної послідовності-мішені в аналізі з РТОСЕ (розщепленням та подовженням РТО). В даному винаході здійснюють детекцію нуклеїновокислотної послідовності-мішені, при цьому РТО (олігонуклеотид, що зондує та мітить), гібридизований з нуклеїновокислотною послідовністю-мішенню, розщеплюється з вивільненням фрагмента, і цей фрагмент гібридизується з СТО (захоплюючим та матричним олігонуклеотидом) з утворенням подовженого дуплекса, після чого відбувається детекція присутності подовженого дуплекса. Подовжений дуплекс забезпечує одержання сигналів (генерацію, підсилення, гасіння або ослаблення сигналів) від міток, що вказують на присутність подовженого дуплекса, і має регульовану величину Т, яка є добре придатною для детекції присутності нуклеїновокислотної послідовності-мішені.The invention relates to a method for detecting the target nucleic acid sequence in an assay with RTOS (cleavage and elongation of RT). In the present invention carry out the detection of the nucleic acid sequence of the target, with RTO (probing and tagging oligonucleotide), hybridized with the nucleic acid target sequence, split with the release of the fragment, and this fragment is removed from the fragment and , followed by the detection of the presence of an elongated duplex. The elongated duplex provides signals (generation, amplification, attenuation, or attenuation of signals) from labels indicating the presence of an elongated duplex, and has an adjustable value T that is well suited for detecting the presence of a target nucleic acid sequence.

UAA201309884A 2011-01-11 2012-11-01 Detection of nucleic acid sequences of the target in the analysis of the splitting and extension pto UA110815C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
UAA201508100A UA115082C2 (en) 2011-01-11 2012-01-11 METHOD OF DETECTION OF NUCLEIC ACID SEQUENCE-DIRECTED IN ANALYSIS OF FLEXIBLE SPLITTING AND EXTENSION

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110002840A KR20120081482A (en) 2011-01-11 2011-01-11 Detection of target nucleic acid sequence by tagged probe cleavage and extension
PCT/KR2012/000287 WO2012096523A2 (en) 2011-01-11 2012-01-11 Detection of target nucleic acid sequences by pto cleavage and extension assay

Publications (1)

Publication Number Publication Date
UA110815C2 true UA110815C2 (en) 2016-02-25

Family

ID=46713623

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA201309884A UA110815C2 (en) 2011-01-11 2012-11-01 Detection of nucleic acid sequences of the target in the analysis of the splitting and extension pto

Country Status (2)

Country Link
KR (1) KR20120081482A (en)
UA (1) UA110815C2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6050555B2 (en) * 2013-07-15 2016-12-21 シージーン アイエヌシー Detection of target nucleic acid sequences using PTO cleavage and extension-dependent immobilized oligonucleotide hybridization
KR102218776B1 (en) * 2016-06-10 2021-02-22 주식회사 씨젠 Method of providing tagging oligonucleotide
CN108823287B (en) 2017-04-28 2019-04-12 厦门大学 A method of detection target nucleic acid sequence
KR102097721B1 (en) * 2019-01-24 2020-04-06 주식회사 시선바이오머티리얼스 Method for Detecting Multiple Target Based on Single Detection Probe using Tag sequence SNP
WO2020256378A1 (en) * 2019-06-17 2020-12-24 강릉원주대학교산학협력단 Nucleic acid detection method

Also Published As

Publication number Publication date
KR20120081482A (en) 2012-07-19

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