AR080913A1 - PRIMERS, PROBES, METHODS AND SETS OF ELEMENTS FOR THE DETECTION OF EML4-ALK VARIANTS - Google Patents
PRIMERS, PROBES, METHODS AND SETS OF ELEMENTS FOR THE DETECTION OF EML4-ALK VARIANTSInfo
- Publication number
- AR080913A1 AR080913A1 ARP110101303A AR080913A1 AR 080913 A1 AR080913 A1 AR 080913A1 AR P110101303 A ARP110101303 A AR P110101303A AR 080913 A1 AR080913 A1 AR 080913A1
- Authority
- AR
- Argentina
- Prior art keywords
- seq
- eml4
- alk
- sample
- variant
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Hospice & Palliative Care (AREA)
- Biophysics (AREA)
- Oncology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Cebadores, sondas y conjuntos de elementos (kits) para la deteccion de secuencias variantes de EML4-ALK en una muestra y métodos de deteccion de variantes de EML4-ALK usando los cebadores, sondas y conjuntos de elementos que se divulgan. Reivindicacion 1: Un método para detectar la presencia de una variante EML4-ALK en una muestra, caracterizado porque dicho método comprende: (a) aislar un ácido nucleico de dicha muestra, en donde la muestra comprende secuencias de ADN; (b) conducir una reaccion de amplificacion de dichas secuencias de ADN de dicha muestra, en donde dicha reaccion de amplificacion comprende un primer cebador capaz de alinearse específicamente con una secuencia de una variante EML4-ALK en una primera posicion en una secuencia de ADN de EML4-ALK, en donde dicho primer cebador se selecciona del grupo que consiste de la SEQ ID N°:1, la SEQ ID N°:2, la SEQ ID N°:3, la SEQ ID N°:4, la SEQ ID N°:5, la SEQ ID N°:6, la SEQ ID N°:7, la SEQ ID N°:8, la SEQ ID N°:9, la SEQ ID N°:12, la SEQ ID N°:13, la SEQ ID N°:14, la SEQ ID N°:15, la SEQ ID N°:16, la SEQ ID N°:17, la SEQ ID N°:18, la SEQ ID N°:19, la SEQ ID N°:20, la SEQ ID N°:21, la SEQ ID N°:22, la SEQ ID N°:23, la SEQ ID N°:24 y la SEQ ID N°:25, y un segundo cebador capaz de alinearse específicamente en una segunda posicion en una secuencia de ADN de EML4-ALK, en donde dichos primer y segundo cebadores se alinean con diferentes cadenas de secuencias de ADN de cadena doble de EML4-ALK, en donde la reaccion de amplificacion tiene la capacidad de producir un producto de amplificacion específico de la variante EML4-ALK cuando las secuencias de ADN de la muestra comprenden una secuencia de ADN de EML4-ALK que comprende una secuencia de una variante en dicha primera posicion de dicha secuencia de ADN de EML4-ALK; y (c) visualizar los productos de amplificacion producidos por dicha reaccion de amplificacion, en donde la deteccion de un producto de amplificacion específico de la variante EML4-ALK constituye un indicador positivo de una variante EML4-ALK en dicha muestra.Primers, probes and element sets (kits) for the detection of EML4-ALK variant sequences in a sample and methods of detection of EML4-ALK variants using the primers, probes and element sets disclosed. Claim 1: A method for detecting the presence of an EML4-ALK variant in a sample, characterized in that said method comprises: (a) isolating a nucleic acid from said sample, wherein the sample comprises DNA sequences; (b) conducting an amplification reaction of said DNA sequences of said sample, wherein said amplification reaction comprises a first primer capable of specifically aligning with a sequence of an EML4-ALK variant in a first position in a DNA sequence of EML4-ALK, wherein said first primer is selected from the group consisting of SEQ ID N °: 1, SEQ ID N °: 2, SEQ ID N °: 3, SEQ ID N °: 4, SEQ ID N °: 5, SEQ ID N °: 6, SEQ ID N °: 7, SEQ ID N °: 8, SEQ ID N °: 9, SEQ ID N °: 12, SEQ ID N °: 13, SEQ ID N °: 14, SEQ ID N °: 15, SEQ ID N °: 16, SEQ ID N °: 17, SEQ ID N °: 18, SEQ ID N °: 19, SEQ ID N °: 20, SEQ ID N °: 21, SEQ ID N °: 22, SEQ ID N °: 23, SEQ ID N °: 24 and SEQ ID N °: 25, and a second primer capable of specifically aligning in a second position in an EML4-ALK DNA sequence, wherein said first and second primers align with different strands of chain DNA sequences. double of EML4-ALK, where the amplification reaction has the ability to produce a specific amplification product of the EML4-ALK variant when the DNA sequences of the sample comprise an EML4-ALK DNA sequence comprising a sequence of a variant in said first position of said EML4-ALK DNA sequence; and (c) visualizing the amplification products produced by said amplification reaction, wherein the detection of a specific amplification product of the EML4-ALK variant constitutes a positive indicator of an EML4-ALK variant in said sample.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US32484010P | 2010-04-16 | 2010-04-16 | |
| US35141910P | 2010-06-04 | 2010-06-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR080913A1 true AR080913A1 (en) | 2012-05-16 |
Family
ID=44799341
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP110101303 AR080913A1 (en) | 2010-04-16 | 2011-04-15 | PRIMERS, PROBES, METHODS AND SETS OF ELEMENTS FOR THE DETECTION OF EML4-ALK VARIANTS |
Country Status (3)
| Country | Link |
|---|---|
| AR (1) | AR080913A1 (en) |
| TW (1) | TW201202703A (en) |
| WO (1) | WO2011130635A2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101987065B1 (en) * | 2017-08-07 | 2019-06-10 | 주식회사 싸이토젠 | A method for analyzing eml4-alk gene variance |
| CN109022581A (en) * | 2018-08-13 | 2018-12-18 | 上海佰臻生物科技有限公司 | The kit of EML4-ALK fusion fluorescence quantitative PCR detection |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL2450437T3 (en) * | 2006-04-14 | 2017-12-29 | Cell Signaling Technology Inc | Gene defects and mutant ALK kinase in human solid tumors |
| CA2598893C (en) * | 2006-10-11 | 2012-04-10 | Astellas Pharma Inc. | Eml4-alk fusion gene |
| AU2009215168A1 (en) * | 2008-02-12 | 2009-08-20 | Dana-Farber Cancer Institute | Fish assay for EML4 and ALK fusion in lung cancer |
-
2011
- 2011-04-15 AR ARP110101303 patent/AR080913A1/en unknown
- 2011-04-15 WO PCT/US2011/032694 patent/WO2011130635A2/en active Application Filing
- 2011-04-15 TW TW100113162A patent/TW201202703A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2011130635A2 (en) | 2011-10-20 |
| TW201202703A (en) | 2012-01-16 |
| WO2011130635A3 (en) | 2011-12-29 |
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