TWM626410U - Cell culture apparatus - Google Patents

Abstract

The present invention provides a cell culture equipment, comprising a reaction part, a testing part and a cell culture device arranged in the testing part. The parameter monitoring department of the trial department. In the cell culture device of the present invention, the test part is connected in parallel with the reaction part, so that a circulation control path is formed between the circulation part and the reaction part, and the reaction state can be obtained through the parameter monitoring part provided in the test part The process of the parameters will not affect the cell culture process in the reaction part, thereby reducing the crowding of the cell culture space and facilitating the rapid and accurate addition or replacement of the nutrient solution.

Description

cell culture equipment

The new model relates to the field of biotechnology, in particular to cell culture equipment.

Whether static cell culture or dynamic cell culture is carried out, avoiding the problem that cells cannot get a favorable growth space and get sufficient material exchange during the culture process due to local cell density, and it is helpful to improve the activity and function of cells. Therefore, monitoring the reaction parameters of the culture process is particularly important.

Chinese Patent Publication No. CN102199537B discloses a bioreactor device, the bioreactor device includes a parameter measurement and control system arranged on the main body of the reactor, which can monitor reaction parameters including temperature, pH value, dissolved oxygen and pressure in real time . However, in this technical solution, the parameter measurement and control system for monitoring all reaction parameters is arranged on the main body of the reactor, which occupies space for cell culture and is not conducive to fast and accurate addition or replacement of nutrient solution.

Therefore, it is necessary to design a new type of cell culture equipment to solve the above problems existing in the prior art.

The purpose of the present invention is to provide a cell culture device, which reduces the occupation of the cell culture space and facilitates the rapid and accurate addition or replacement of nutrient solution.

In order to achieve the above purpose, the cell culture device of the present invention includes a reaction part to provide a cultivation place for seed cells, and further includes a test part and a parameter monitoring part arranged in the test part; the test part is connected in parallel with the reaction part, so that the A circulation control passage is formed between the circulation part and the reaction part, so that the flow medium in the reaction part flows between the test part and the reaction part, and the reaction state parameters are obtained through the parameter monitoring part.

The beneficial effect of the novel cell culture equipment is that: the cell culture equipment includes a testing part and a parameter monitoring part arranged in the testing part, the testing part is connected in parallel with the reaction part, so that the circulation part and the reaction part are formed between the circulation part and the reaction part. The process of obtaining the reaction state parameters through the parameter monitoring part will not affect the cell culture process carried out in the reaction part, thereby reducing the occupation of the cell culture space and facilitating the rapid and accurate addition or replacement of nutrient solution.

Preferably, the circulation control path includes a shunt path and a return path, two ends of the shunt path are respectively connected to the top of the test part and the top of the reaction part, and two ends of the return path are respectively connected to the top of the test part The bottom and the side wall or bottom of the reaction section.

Further preferably, the cell culture apparatus further includes a liquid level detection part, and the liquid level detection part is arranged in the reaction part to obtain the liquid level information of the liquid flowing medium in the reaction part.

Further preferably, the cell culture apparatus further includes a reaction driving part disposed in the reaction part to drive the liquid flow medium in the reaction part to move. The beneficial effect is that more sufficient material exchange can be achieved through dynamic cell culture.

Further preferably, the cell culture equipment further includes a waste liquid collection part, and the waste liquid collection part is connected to the reaction part.

Further preferably, the cell culture device further includes a liquid supply part for supplying liquid nutrients, and the liquid supply part is connected to the reaction part.

Further preferably, the reaction part is further provided with a metering display part to assist in confirming the amount of the flowing medium in the reaction part.

Further preferably, the cell culture equipment further includes a gas supply part for supplying gaseous nutrients, and the gas supply part is connected to the reaction part.

Further preferably, the cell culture equipment further includes a temperature regulation part, and the temperature regulation part covers the test part and the reaction part to adjust the reaction temperature.

Further preferably, it further includes a control part, which is connected to the circulation control passage, the reaction driving part, the parameter monitoring part, the liquid level detection part, the liquid supply part, the gas supply part, the metering display part, the At least one of the waste liquid collecting part and the temperature adjusting part is controlled in a corresponding mode according to the reaction state parameter and the liquid level element information.

Further preferably, the cell culture apparatus further includes a storage unit connected to the control unit, and the storage unit stores culture mode information for the control unit to call and automatically control the corresponding mode.

Preferably, the parameter monitoring part includes at least one of a temperature sensing part, a pressure sensing part, a pH monitoring part, a substance content monitoring part and a dissolved oxygen monitoring part.

Preferably, the cell culture equipment further comprises an exhaust gas condensation part disposed in the reaction part to assist the liquid flow medium in the reaction part to achieve condensation and reflux.

Preferably, the cell culture equipment further includes a sampling part disposed on at least one of the reaction part and the testing part.

Preferably, the cell culture device further comprises a liquid replenishing part connected to the reaction part.

13: Parameter Monitoring Department

14: Liquid level detection department

15: Reaction Drive

16: Measurement display part

110: Loop body

111: Return path

112: Backflow Control Department

120: React Body

121: Shunt Path

122: Shunt Control Department

210: Liquid supply body

211: Liquid supply channel

212: Liquid supply switch part

213: Liquid supply control department

220: Air supply main body

221: Air supply passage

222: Air supply switch part

223: Air Supply Control Department

310: Recycling the main body

311: Recycling Channel

312: Recycling Switch Department

410: Main body of rehydration

411: Rehydration switch department

412: Rehydration channel

420: Sampling Department

Fig. 1 is a schematic structural diagram of the cell culture equipment of the novel embodiment 1; Fig. 2 is a structural schematic diagram of the cell culture equipment of the novel embodiment 2; Fig. 3 is a structural schematic diagram of the cell culture equipment of the novel embodiment 3; Fig. 4 This is a schematic diagram of the structure of the cell culture equipment of Example 4 of the present novel.

In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. example. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention. Unless otherwise defined, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this new invention belongs. As used herein, "comprising" and similar words mean that the elements or things appearing before the word encompass the elements or things recited after the word and their equivalents, but do not exclude other elements or things.

In view of the problems existing in the prior art, the novel embodiment provides a cell culture device, which includes a reaction part and a test part. Wherein the reaction part provides a cultivation place for seed cells.

The beneficial effects of the present invention will be described below through specific embodiments.

Example 1:

FIG. 1 is a schematic structural diagram of the cell culture apparatus of the novel embodiment 1. FIG.

In Example 1 of the present invention, the test part is connected in parallel with the reaction part, so that the circulation part is A circulation control passage including a branch passage and a return passage is formed between the reaction part and the reaction part.

Specifically, referring to FIG. 1 , the circulation main body 110 , the return passage 111 and the backflow regulating part 112 arranged in the return passage 111 constitute the testing part; The shunt regulation part 122 of the 122 constitutes the reaction part.

Two ends of the shunt passage 121 and two ends of the return passage 111 are respectively connected to the circulation main body 110 and the reaction main body 120 , so as to realize the parallel connection of the circulation main body 110 and the reaction main body 120 , so as to facilitate the internal connection of the reaction main body 120 The flow medium of 100°C circulates between the circulation main body 110 and the reaction main body 120 through the shunt passage 121 and the return passage 111 .

The flow medium is liquid nutrients and gaseous nutrients required for cell culture of the seed cells.

The reaction body 120 can accommodate seed cells and nutrients for cell culture of the seed cells. In some specific embodiments of the present invention, the reaction main body 120 is a cell culture flask.

In some embodiments of the present invention, after the seed cells are loaded on the carrier, they are accommodated in the reaction body 120 . Specifically, the carrier is a slide, and the seed cells are primary cells.

In some embodiments of the present invention, the seed cells are adherent cells, and are directly loaded on the bottom of the reaction body 120 or the sidewall near the bottom.

In some embodiments of the present invention, the cell type of the seed cells is hepatocytes or stem cells.

Referring to FIG. 1 , the shunt regulation part 122 and the backflow regulation part 112 respectively control the conduction state of the shunt passage 121 and the backflow passage 111 and the circulation control of the flow medium between the circulation main body 110 and the reaction main body 120 Flow conditions in passages (not shown). In some specific embodiments of the present invention, the shunt regulating part 122 and the backflow regulating part 112 are both the peristaltic pump and the metering pump. meaning one.

In the first embodiment of the present invention, the parameter monitoring part is provided in the testing part. Specifically, referring to FIG. 1 , the parameter monitoring unit 13 is disposed on the test body 110 , when the circulation control passage (not marked in the figure) is in the conducting state, part of the flowing medium of the reaction body 120 is in the reaction body 120 and the circulation body 110 is in a continuous flow state, the reaction state parameter obtained by the parameter monitoring part 13 reflects the reaction state parameter in the reaction main body 120, and the monitoring process will not affect the cell culture process in the reaction main body 120, Thus, the occupancy of the cell culture space is reduced and the continuity of the cell culture process is not affected.

In some embodiments of the present invention, the parameter monitoring part 13 includes at least one of a temperature sensing part, a pressure sensing part, a pH monitoring part, a substance content monitoring part and a dissolved oxygen monitoring part.

Specifically, the substance content monitoring unit is used to monitor and analyze the content of nutrients or metabolites. The nutrient is the nutrient contained in the liquid nutrient; the metabolite is the metabolite produced by the seed cell after the cell is cultured.

In some embodiments of the present invention, the parameter monitoring part 13 includes at least two of the temperature sensing part, the pressure sensing part, the pH monitoring part, the substance content monitoring part and the dissolved oxygen monitoring part.

In the first embodiment of the present invention, the parameter monitoring part 13 is composed of a temperature sensing part, the pressure sensing part, the pH monitoring part, the nutrient content monitoring part and the dissolved oxygen monitoring part.

Specifically, the temperature sensing part is a temperature sensor; the pressure sensing part is a pressure sensor, and the pH monitoring part is a pH electrode; the substance content monitoring part is a bioanalyzer capable of detecting glucose content and lactic acid content ; The dissolved oxygen monitoring unit is an online dissolved oxygen analyzer.

In the first embodiment of the present invention, the liquid level detection part is arranged in the reaction part. Referring to FIG. 1 , the liquid level detection part 14 is disposed on the reaction main body 120 , and specifically penetrates through the top of the reaction main body 120 to be connected with the reaction main body 120 . The liquid medium in the reaction body 120 is in contact with each other to obtain the liquid level element information.

In the first embodiment of the present invention, the reaction part is provided with a reaction driving part. Referring to FIG. 1 , the reaction driving part 15 is disposed at the bottom of the reaction main body 120 to drive the movement of the liquid flow medium in the reaction main body 120 to achieve more sufficient material exchange through dynamic cell culture.

In some specific embodiments of the present invention, the reaction driving part 15 is a magnetic stirrer.

In some embodiments of the present invention, the reaction driving part 15 is disposed on the side wall of the reaction body 120 to drive the liquid substance in the reaction body 120 to move.

In the first embodiment of the present invention, the reaction part is further provided with a metering display part. Referring to FIG. 1 , the metering display part 16 is disposed below the reaction main body 120 and supports the reaction main body 120 and the reaction driving part 15 to help confirm the amount of the flowing medium in the reaction main body 120 .

In some specific embodiments of the present invention, the measurement display part 16 is a digital display balance.

The cell culture equipment of the new embodiment 1 is applied in the process of cell culture: The adherent cells are loaded on the side wall surface of the reaction main body 120, and the liquid nutrients immerse the adherent cells; the top of the reaction main body 120 communicates with the external environment, and is provided with a bacteriostatic membrane to isolate harmful substances, so as to provide the required cells for cell culture. Gaseous nutrients.

After the circulation control passage (not marked in the figure) between the circulation main body 110 and the reaction main body 120 is activated, part of the liquid nutrients in the reaction main body 120 is driven to the circulation main body 110 along the circulation control passage (not marked in the figure) and the reaction main body 120; by respectively controlling the shunt regulating part 122 and the backflow regulating part 112 to adjust the flow rate or flow of the circulating liquid nutrients, the circulating liquid nutrients can be periodically reciprocated in the circulating main body 110 and the circulating liquid nutrients. The reaction main body 120, so that the liquid nutrients in the reaction main body 120 are periodically immersed in the adherent cells, so as to carry out the cell culture.

During the process of the cell culture, due to the circulation body 110 and the reaction body 120 They communicate with each other, and part of the liquid nutrients are reciprocally circulated in the circulation main body 110 and the reaction main body 120, and the temperature parameters, pressure parameters, pH monitoring parts, glucose content parameters and dissolved oxygen content parameters obtained by the parameter monitoring part 13 can reflect the The reaction situation in the reaction body 120 .

Example 2:

FIG. 2 is a schematic structural diagram of the cell culture apparatus of the novel embodiment 2. FIG.

In the second embodiment of the present invention, the cell culture device further includes a liquid supply part for providing liquid nutrients, and the liquid supply part is connected to the reaction part to provide the liquid nutrients to the reaction part.

Specifically, referring to FIG. 1 and FIG. 2 , the difference between the cell culture apparatus of Example 2 and the cell culture apparatus of Example 1 is that the cell culture apparatus shown in FIG. 2 further includes a liquid supply body 210 , a liquid supply passage 211 and The liquid supply part composed of the liquid supply switch part 212 and the liquid supply control part 213 provided in the liquid supply passage 211 .

The two ends of the liquid supply passage 211 are respectively connected to the liquid supply main body 210 and the reaction main body 120 , so that the liquid nutrients stored in the liquid supply main body 210 are transported to the liquid supply main body 210 through the liquid supply switch part 212 and the liquid supply control part 213 inside the reaction body 120 .

In the second embodiment of the present invention, the cell culture equipment further includes an air supply part for supplying gaseous nutrients, and the air supply part is connected to the reaction part.

Specifically, referring to FIG. 1 and FIG. 2 , the difference between the cell culture apparatus of Example 2 and the cell culture apparatus of Example 1 is that the cell culture apparatus shown in FIG. 2 further includes an air supply main body 220 and an air supply passage 221 And the air supply part formed by the air supply switch part 222 and the air supply control part 223 arranged in the air supply passage 221 .

Both ends of the gas supply passage 221 are respectively connected to the gas supply main body 220 and the reaction main body 120 , so that the gaseous gas stored in the gas supply main body 220 can be stored in the gas supply main body 220 through the gas supply switch part 222 and the gas supply control part 223 . Nutrients are transported into the reaction body 120 .

The liquid supply switch part 212 and the air supply switch part 222 respectively control the conduction state of the liquid supply passage 211 and the air supply passage 221 . In some specific embodiments of the present invention, the liquid supply switch part 212 and the gas supply switch part 222 are either a shut-off valve or an on-off valve.

The liquid supply control part 213 and the gas supply control part 222 respectively control the flow rate or flow rate of liquid nutrients and gaseous nutrients entering the reaction main body 120 . In some specific embodiments of the present invention, the liquid supply control part 213 and the air supply control part 222 are either a peristaltic pump or a metering pump.

Example 3:

FIG. 3 is a schematic structural diagram of the cell culture device of the novel embodiment 3. FIG.

In the third embodiment of the present invention, the cell culture equipment further includes a waste liquid collection part, and the waste liquid collection part is connected to the reaction part.

Specifically, referring to FIGS. 2 and 3 , the difference between the cell culture part of Example 3 and the cell culture part of Example 2 is that the cell culture device shown in FIG. 3 further includes a recovery main body 310 , a recovery channel 311 and a The waste liquid collection part formed by the recovery switch part 312 of the recovery channel 311 .

The two ends of the recovery channel 311 are respectively connected to the reaction main body 120 and the recovery main body 310. When the parameter monitoring part 13 or the liquid level element detection part 14 judges that it is necessary to replace or increase the liquid nutrients in the reaction main body 120, At least part of the liquid in the reaction main body 120 is recovered into the recovery main body 310 by controlling the recovery switch part 312 , and the measurement display part 16 assists in confirming the amount of liquid substances entering the recovery main body 310 .

After the recovery is completed, the recovery passage 311 is closed by controlling the recovery switch 312, and then the liquid supply body 210 provides liquid nutrients to the reaction body 120, and the metering display portion 16 assists in confirming that the reaction is entered. The amount of liquid substance of the body 120 .

In some embodiments of the present invention, the recovery switch part 312 controls the conduction state of the recovery passage 311 . In some specific embodiments of the present invention, the recovery switch portion 312 is any one of a shut-off valve and an on-off valve.

Example 4:

In the fourth embodiment of the present invention, the cell culture equipment further includes a liquid replenishing part.

Specifically, the fluid replenishing part is composed of a fluid replenishing main body, a fluid replenishing channel and a fluid replenishing switch part.

FIG. 4 is a schematic structural diagram of the cell culture apparatus of the novel example 4. FIG.

Referring to FIGS. 3 and 4 , the difference between the cell culture apparatus shown in FIG. 4 and the cell culture apparatus shown in FIG. 3 is that it further includes a fluid supplement body 410 , a fluid supplement channel 412 connecting the fluid supplement body 410 and the reaction body 120 , and The rehydration switch part 411 is arranged on the rehydration channel 412 ; in addition, the top of the reaction main body 120 is also provided with a sampling part 420 .

After the liquid substance in the reaction body 120 is acquired by the sampling part 420 for analysis and monitoring, the fluid replacement body 410 and the fluid replacement switch part 411 need to be operated to make supplementary substances enter the reaction body 120 so as not to affect the normal substance exchange.

Specifically, the liquid replenishing body 410 is a soft-shell medical container, and the replenishing substance can be entered into the reaction body 120 by manual squeezing.

Specifically, the fluid supplement switch part 411 is any one of a shut-off valve and an on-off valve.

In some embodiments of the present invention, the cell culture apparatus further includes a temperature regulation part, and the temperature regulation part covers the test part and the reaction part to adjust the reaction temperature.

Specifically, the temperature adjustment part is a closed cover body with a temperature adjustment device installed, and the circulation main body 110 , the reaction main body 120 and all the passages connecting the circulation main body 110 and the reaction main body 120 are accommodated in the closed cover body , to control the temperature at which the cell culture is carried out by the temperature regulating device Spend.

In some embodiments of the present invention, the cell culture system further includes a control part, which is connected to the circulation control channel, the reaction driving part, the parameter monitoring part, the liquid level detection part, the liquid supply part, and the gas supply part , at least one of the metering display part, the waste liquid collecting part and the temperature adjusting part to perform corresponding mode control according to the reaction state parameter and the liquid level element information.

In some specific embodiments of the present invention, the control part is electrically connected to the parameter monitoring part 13 , the liquid level detection part 14 , the reaction driving part 15 , the metering display part 16 , the shunt control part 122 , and the backflow control part 112 , at least one of the liquid supply switch part 212 , the liquid supply control part 213 , the air supply switch part 222 , the air supply control part 223 and the recovery switch part 312 .

In some embodiments of the present invention, the cell culture apparatus further includes a storage unit connected to the control unit, and the storage unit stores culture mode information for the control unit to call and automatically control the corresponding mode.

In some embodiments of the present invention, the cell culture apparatus further includes an exhaust gas condensing part disposed in the reaction part to assist the liquid flow medium in the reaction part to achieve condensation and reflux.

Although the embodiments of the present invention have been described in detail above, it will be apparent to those skilled in the art that various modifications and changes can be made to these embodiments. However, it should be understood that such modifications and changes fall within the scope and spirit of the present invention as set forth in the claims. Furthermore, the invention described herein is capable of other embodiments and of being practiced or carried out in various ways.

120: React Body

210: Liquid supply body

410: Main body of rehydration

411: Rehydration switch department

412: Rehydration channel

420: Sampling Department

Claims (15)

A cell culture equipment, comprising: a reaction part to provide a cultivation place for seed cells; a test part; and a parameter monitoring part, arranged in the test part, wherein the test part is connected in parallel with the reaction part to make a cycle A circulation control passage is formed between the reaction part and the reaction part, so that the flow medium in the reaction part circulates between the test part and the reaction part, and a reaction state parameter is obtained through the parameter monitoring part. The cell culture device according to claim 1, wherein the circulation control passage comprises a shunt passage and a return passage, two ends of the shunt passage are respectively connected to the top of the test part and the top of the reaction part, and the return passage Two ends of the flow passage are respectively connected to the bottom of the test part and the side wall or bottom of the reaction part. The cell culture apparatus according to claim 1, further comprising a liquid level detection part, the liquid level detection part is disposed in the reaction part to obtain a liquid level information of the liquid flowing medium in the reaction part. The cell culture apparatus according to claim 3, further comprising a reaction driving part disposed in the reaction part to drive the liquid flow medium in the reaction part to move. The cell culture apparatus according to claim 4, further comprising a waste liquid collection part, and the waste liquid collection part is connected to the reaction part. The cell culture apparatus according to claim 5, further comprising a liquid supply part for supplying liquid nutrients, the liquid supply part being connected to the reaction part. The cell culture device according to claim 6, wherein the reaction part is further provided with a The metering display unit is used to assist in confirming the amount of the fluid medium contained. The cell culture apparatus according to claim 7, further comprising an air supply part for supplying gaseous nutrients, the air supply part being connected to the reaction part. The cell culture apparatus according to claim 8, further comprising a temperature-adjusting part, the temperature-adjusting part covers the testing part and the reaction part to adjust the reaction temperature. The cell culture apparatus according to claim 9, further comprising a control unit, the control unit is connected to the circulation control passage, the reaction driving unit, the parameter monitoring unit, the liquid level detection unit, the liquid supply unit, and the gas supply At least one of the part, the measurement display part, the waste liquid collection part and the temperature adjustment part, so as to perform corresponding mode control according to the reaction state parameter and the liquid level element information. The cell culture apparatus according to claim 10, further comprising a storage unit connected to the control unit, the storage unit stores culture mode information for the control unit to call and automatically control the corresponding mode. The cell culture apparatus according to claim 1, wherein the parameter monitoring part includes at least one of a temperature sensing part, a pressure sensing part, a pH monitoring part, a substance content monitoring part and a dissolved oxygen monitoring part. The cell culture apparatus according to claim 1, further comprising an exhaust condensation part disposed in the reaction part to assist the liquid flow medium in the reaction part to achieve condensation and reflux. The cell culture apparatus according to claim 1, further comprising a sampling part disposed on at least one of the reaction part and the testing part. The cell culture apparatus according to claim 14, further comprising a liquid replenishing part connected to the reaction part.

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