CN206014941U - A kind of organism culturing device based on hemodialyzer - Google Patents

Abstract

This utility model provides a kind of organism culturing device based on hemodialyzer, including hemodialyzer, circulating pump, wave weighing device, sample collecting storage device, doser, culture bag, described culture bag includes main culture bag and time culture bag, the inside two ends of described main culture bag and hemodialyzer main body are connected to form main circulation loop, the outside two ends of described secondary culture bag and hemodialyzer main body are connected to form time closed circuit, and described sample collecting storage device, doser are connected with the pipeline between main culture bag and hemodialyzer.This utility model provide a kind of can monitor and maintain condition of culture stable, automatic liquid-feeding and sample, and can be with aids drug concentration change, the cell or germ culturing device based on hemodialyzer.

Description

A kind of organism culturing device based on hemodialyzer

Technical field

This utility model is related to a kind of biological culture field, more particularly to a kind of biological culture based on hemodialyzer Device.

Background technology

Stem cell culture is referred to simulates internal physiological environment in vitro, cultivates the cell taken out from body, and is allowed to raw The technology that deposits and grow.Cell culture invitro must be noted that three links：The exclusion of nutrition, living environment and waste liquid.External training Nutrition needed for foster cell is provided by culture medium.In vitro culture must simulate cells in vivo growing environment.Environmental factorss master Gnotobasiss to be referred to, suitable temperature, certain osmotic pressure and gaseous environment.Stem cell is a kind of self renewal, height increasing Grow and multiple differentiation potentials undifferentiated cell.The generation of various functions cell and regulation and control depend on various microenvironments, thin The intracellular growth factor, stromal cell, the interaction of extracellular matrix layer many factors and balance, and it is related to relevant cell proliferation, Differentiation, migration are settled down, the fundamental mechanism in the life sciences such as aging, apoptosis, canceration.Stem cell culture method now includes Including culture bag culture, culture bottle（Tank）Culture and hollow fiber reactor culture etc., no matter any cultural method is required for Culture medium is constantly changed, or carries out the renewed vaccination of stem cell, to maintain the growth conditionss and somatomedin of stem cell, taken When bothersome expensive, and easily cause pollution.

Medicine PK/ PD research be the drug research that newly-developed gets up important method, PK therein（Medicine is for power Learn）It is effect of the body to medicine, including the dynamic changing process of medicine absorption,distribution,metabolism,excretion in vivo.PD（Medicine Effect is learned）It is medicine to body（Including pathogen）Effect, including the treatment that certain density medicine is produced to body（Or to disease The killing of substance）Effect and untoward reaction.PK/PD research be PK and PD combine research drug dose corresponding " when Between drug level body effect " relation, for antimicrobial drug, then can reflect between " medicine body pathogen " Relation.No matter pharmacokineticss and pharmacodynamicss are only combined, could disclose drug concentration change situation by which kind of medicine Under curative effect or bactericidal activity, so as to formulate effective therapeutic scheme, reach optimal clinical and bacteriological identification.Current PK/PD research methoies, the method for adopting loose mail to combine, including culture bottle more（Or hollow fiber reactor）, pump, DL device, mistake Filter, incubator etc..The dynamic analog of particularly medicine change is difficult to realize, the control of condition of culture（With stem cell culture one Sample）Comparatively laborious.

Utility model content

Utility model purpose：For the demand of above-mentioned medical research, based on overcoming defect present in prior art, this Utility model aim to provide a kind of can monitor and maintain condition of culture stable, automatic liquid-feeding and sampling, and can be with mould Intend drug concentration change, cell or germ culturing device based on hemodialyzer.

Technical scheme

A kind of organism culturing device based on hemodialyzer, including hemodialyzer, circulating pump, wave weighing device, Sample collecting storage device, doser, culture bag, described culture bag include main culture bag and time culture bag, described master The inside two ends of culture bag and hemodialyzer main body are connected to form main circulation loop, described secondary culture bag and hemodialyzer The outside two ends of main body are connected to form time closed circuit, and described circulating pump includes main circulation pump and time circulating pump, described master , on the pipeline between main culture bag and hemodialyzer, described secondary circulating pump is saturating located at time culture bag and blood for circulating pump On pipeline between parser, the described weighing device that waves includes that master waves weighing device and waves weighing device with secondary, described Master waves weighing device located at the lower section of main culture bag, and the described secondary weighing device that waves is located at the lower section of time culture bag, described Sample collecting storage device, doser is connected with the pipeline between main culture bag and hemodialyzer.

Described hemodialyzer is made up of capillary fiber pipe and shell, and the inside of described hemodialyzer main body refers to The inside of capillary fiber pipe, the outside of described hemodialyzer refer to the space between shell and capillary fiber pipe.

Further technical scheme, described doser include medicinal bag and syringe pump, and described medicinal bag is by note The pipeline that penetrates between pump and main culture bag and hemodialyzer is connected.

Further technical scheme, described circulating pump is peristaltic pump.

Further technical scheme, the described weighing device that waves include waving machine support, motor, wave balancing frame, support Disk, LOAD CELLS, located at waving on machine support, the described balancing frame that waves includes roller bearing and balancing frame, institute to described motor The roller bearing that states is connected with balancing frame；Described motor is connected with roller bearing by motor connection axle；Described balancing frame and support Sensor stand is provided between disk, is provided with LOAD CELLS, described LOAD CELLS and pallet in described sensor stand It is connected.

Further technical scheme, described waving be additionally provided with machine support fixed column, described fixed column and roller bearing phase Connection；Described sensor stand is detachably connected with balancing frame；

Described balancing frame is provided with three equilibrium points；

Described roller bearing is three, and one of them is connected with motor connection axle；

Three described roller bearings are placed in isosceles triangle, highly identical and parallel with waving machine support；；

Described pallet is slant setting, and described range of tilt angles is 5 ° -30 °；

Culture bag is additionally provided with described pallet, and the inlet/outlet pipe of described culture bag is located at tilted-putted culture bag Bottom.

Further technical scheme, described sample collecting storage device include sample collecting apparatus, sample storage device, Cleaning device, two-dimentional machinery injection device and control device；Sample collecting apparatus are connected with two-dimentional machinery injection device, and will pipe The sample liquid extracted in road is injected in two-dimentional machinery injection device；Cleaning device is to the injection needle in two-dimentional machinery injection device Carry out disinfection cleaning；Two-dimentional machinery injection device injects sample liquid in sample storage device；Described control device and collection Device, sample storage device, cleaning device, two-dimentional machinery injection device are connected.

Further technical scheme, described two-dimentional machinery injection device include two-dimentional machinery arm, pin folder and injection needle；Institute The two-dimentional machinery arm that states is connected with injection needle by pin folder, and described two-dimentional machinery arm controls fortune of the injection needle in two-dimensional space Dynamic, described two-dimentional machinery arm is connected with control device；

The sample collecting apparatus include syringe, sampling needle, fixed plate, push-and-pull slide block, V-block, depression bar molectron, straight Line guide rail, lead screw motor, feed screw nut's connecting plate, optocoupler catch, optocoupler, tee T；

The piston handle of described syringe is fixed on line slideway by push-and-pull slide block, and line slideway is fixed on fixation The top of plate；

, on feed screw nut's connecting plate, optocoupler is installed in the middle part of fixed plate for described optocoupler catch；

The staving of described syringe is fixed on the bottom of fixed plate by depression bar molectron, V-block；

Described lead screw motor is connected with push-and-pull slide block by feed screw nut's connecting plate；

Described syringe is connected with the injection needle of sampling needle, two-dimentional machinery injection device by tee T；

Described lead screw motor is connected with control device；

The sample storage device include reducing motor, bearing block, rotation dish, sample box, every bottle stopper plate, sample box Frame；

Described sample box is in rotation dish；

Described reducing motor is connected with sample box support；

Described bearing block is fixed on sample box support；

Described rotation dish is connected with the screw mandrel of reducing motor；

Described reducing motor is connected with control device；

Described is fixed on sample box support every bottle stopper plate, positioned at the surface at sample box center；

The cleaning device includes Washing cup, membrane pump, level sensor, connects liquid bottle, waste liquid bottle；

Described membrane pump includes that the first membrane pump and the second membrane pump, the first described membrane pump are extracted and connect in liquid bottle Cleanout fluid, the waste liquid in described the second membrane pump extraction Washing cup is into waste liquid bottle；

The first described membrane pump and the second membrane pump are connected with control device；

Described level sensor connects the cleanout fluid surplus in liquid bottle located at liquid bottle side is connect for monitoring.

Further technical scheme, described tee T are Y-shaped three-way pipe, described tee T and sampling needle, two-dimentional machine The pipeline of the injection needle connection of tool injection device is provided with pinch valve；Described tee T is fixed by pinch valve, and pinch valve is solid It is scheduled on pipe clamp；Described sample box is provided with sample pore, for placing sample cell；

Also include Hall element in described sample storage device, described Hall element is located at the bottom of sample box Portion, is fixed on sample box mid-stent position on the lower side, and the bottom of sample box is fixed with fritter Magnet；

Described Washing cup electroplate sheath.

Further technical scheme, described organism culturing device also include PH detection means, described PH detection means On pipeline on the pipeline between main culture bag and hemodialyzer and/or between secondary culture bag and hemodialyzer；

Described PH detection means includes LED, LED cover plate, color sensor, color sensor cover plate, bracing frame, On LED cover plate, described color sensor is arranged on color sensor cover plate described LED, described LED Light cover plate and color sensor cover plate are oppositely arranged and are arranged on bracing frame, and described color sensor is by three photoelectric sensings Device is constituted, and is respectively equipped with Red lightscreening plate, green color filter and blue color filter on three photoelectric sensors.

Further technical scheme, described LED and color sensor are located in same horizontal line；Described bracing frame Track is provided with, described LED cover plate and color sensor cover plate are arranged on by slide block on the track of bracing frame；Described Track on bracing frame is provided with draw-in groove.

Further technical scheme, also includes storing culture bag and waste liquid culture bag, and described storage culture bag is by note Penetrate pump to be connected with main culture bag, described waste liquid culture bag is connected with secondary culture bag by syringe pump.

Further technical scheme, described organism culturing device also include control device；Described control device is raw The control module of thing culture apparatuses, control device are used for the entire work flow for controlling biological culture, set up, run, stop including environment Only control, fluid path is set up, runs, stops control, waves the control of weighing device, collect culture base data, sample collecting storage Device starts to sample, stops the operations such as sampling, storage.

Environment is set up includes dual temperature control system, CO2 concentration control systems and ultraviolet lamp disinfection bactericidal unit, and described is double Temperature control system provides two sets of constant temperature systems for device, using refrigeration and heating equipment, by the temperature of sample collecting memory area At 0-4 degree Celsius, the temperature control of other device regions is at 25-37 degree Celsius for control.Ultraviolet lamp disinfection bactericidal unit is Package unit provides exterminating bacterium function.

Described organism culturing device also includes that fluid path pressure-detecting device, described fluid path pressure-detecting device are used for supervising The pressure of liquid in control fluid path circulation, it is ensured that system fluid path is normally run.

Further technical scheme, described main culture bag and time culture bag adopt closed culture, main culture bag and time The material of culture bag is gas permeable material, and described gas permeable material is EVA material or PE materials.

Further technical scheme, described hemodialyzer assume vertical or heeling condition in operation.

Principle of the present utility model：

Dialysis function of this utility model using hemodialyzer, main culture bag and hemodialyzer are connected to form major cycle Loop, secondary culture bag and hemodialyzer are connected to form time closed circuit, carry out nutrient substance by setting up primary and secondary closed circuit Exchange, the power source that peristaltic pump is run as primary and secondary closed circuit, maintain the normal operation of primary and secondary closed circuit.Main circulation loop Fresh culture is extracted from the storage culture bag equipped with fresh culture, is collected in main culture bag, secondary closed circuit is from blood Dialysis culture base in liquid dialyser, finally collects in secondary culture bag through secondary closed circuit, and waving weighing device both can be real When obtain primary and secondary culture bag weight, nutrient substance therein can be shaken up again, most at last waste and old culture fluid by pump from secondary circulation Loop is retracted to waste fluid bag.In the biological incubation such as cell/antibacterial, can be carried out by sample collecting storage device Line sample collecting and storage.Color sensor is used for recognizing culture medium color in primary and secondary closed circuit, so as to judge in culture medium The data such as pH value, judge whether to add fresh culture with this or discharge waste liquid.

Workflow of the present utility model is as follows：

This utility model is used for the biological culture such as cell or antibacterial.

Instrument culture program is set by control device before culture：Set up including environment, run, stop control, fluid path is built Vertical, operation, stop control, wave the control of weighing device, collect culture base data, sample collecting storage device start sampling, Stop the operations such as sampling, storage.

Environment is needed the temperature control of sample collecting memory area at 0-4 degree Celsius in setting up, usually 4 degrees Celsius, The temperature control of other device regions can be adjusted according to demand at 25-37 degree Celsius.Ultraviolet lamp disinfection sterilizing dress It is set to package unit and exterminating bacterium function is provided.

In the original state of the biological culture such as cell or antibacterial, primary and secondary culture bag is separately placed primary and secondary waves and weighs On device, the motor drive roller bearing that starts running for waving weighing device is rotated, and waves balancing frame and title thereon so as to drive Retransmit culture bag on sensor and pallet swinging rotary together.Wherein, weighing data is obtained and can adopt two methods：A kind of Exactly when to be weighed, motor stalls, after obtaining weighing data, motor reruns；Another kind, at the uniform velocity turns in motor In dynamic process, rapid weighing sampling is carried out, obtain meansigma methodss and be its weighing data.In instrument original state primary and secondary culture bag Without culture medium can be moved by peristaltic pump, the culture medium in storage culture bag is pumped in main culture bag, by blood Dialyser enters time closed circuit.When primary and secondary closed circuit set up after, manually in main culture bag injection primary fine cytosol or The biomaterials such as primary fine bacterium solution, if cell culture, then need to inject cytokine, and injection mode has two kinds, and one is to carry Front with culture medium mixing injection, two be shake up primary fine cytosol after, be manually injected into.Cell or antibacterial etc. are biological with waving title Refitting is put and waves mixing entrance main circulation loop, is returned in main circulation loop by hemodialyzer, in main circulation loop Culture medium can enter hemodialyzer and by the Dialysis of fiber surface, enter time closed circuit, and then enter time culture Bag, and mix in the presence of weighing device is waved and survey weight data.According to the result that primary and secondary waves weighing device feedback, Control device adjusts the speed of main circulation pump and time circulating pump.Data are obtained according to pH detection means, judges that culture medium needs more The time that changes, wherein waste and old culture medium are retracted in waste fluid bag from secondary culture bag by pump, are focused on.Then culture bag is stored Fresh culture enters main culture bag by syringe pump, and primary and secondary circulation pump speed is automatically adjusted, to ensure the real-time replacing of culture medium, Until culture is completed.In incubation, the biology such as most cells or antibacterial removes main culture bag i.e. in main culture bag The biologies such as cell or antibacterial can be collected.

Sample collection process of the present utility model：When the circulation fluid path of culture works, control device sends sample collecting and refers to Make to sample collecting apparatus, syringe is taken out automatically and pushes away the mixing for carrying out bio-culture solution twice, then carries out sample collecting, makes With sampling needle sampler into syringe, syringe is again by sample injection to injection needle, you can complete a Reagent Tube sample Acquisition operations, then move injection needle by two-dimentional machinery arm again, complete injection, the storage operation of sample.Sample can be used for carefully The biological count detection such as born of the same parents, antibacterial or the detection for liquor strength.Sample preservation after collection is in 4 degrees Celsius of storage ring In border.

The workflow of pH detection means of the present utility model：By color sensor to culture medium in phenolic red indicator Color detected that obtain in advance the R of culture medium, then G, B value passes through a large amount of test experiments, draw R/G values（Or（R/ G+B/R）Value）Linear relationship between pH value, it is ensured that both dependencys reach 95%.Thus can directly by obtaining R/G values（Or（R/G+B/R）Value）Calculate the pH value of culture medium.When culture medium slant acidity is detected, need to add newly Fresh culture medium, while discharge the waste and old culture medium of equivalent.The opportunity of this liquid feeding discharge opeing is exactly by the monitor value of color sensor Come determined.Operationally, culture medium is present in pipeline drip chamber, when needing to detect color, is first turned on color sensor The R of culture medium is determined, the monitor value of acquisition is drawn the current pH value of culture medium by the linear relationship having built up by G, B value. A part of fresh culture will be supplemented when the pH of culture medium reaches marginal value immediately, while discharging the waste liquid of equivalent, kept The dynamic equilibrium of whole system, improves the work efficiency of whole system.When the pH of culture medium reaches critical point, newly add fresh After culture medium, pH can be raised, and corresponding color can also change, and then the amount of the culture medium for controlling to add, and ensure cell most Tachyauxesis environment.Simultaneously can also be according to the foundation of the variable quantity of color sensor rgb value and the relation of cell density, so as to judge Culture medium metabolic condition determines the output of culture medium.

The operation principle for waving weighing device in this utility model：The rotating speed size of the pump in closed circuit can direct shadow The flow velocity of culture medium in closed circuit is rung, the inside and outside osmotic pressure of hemodialyzer when through hemodialyzer, can be affected, so as to Affect the dialysis direction of main circulation loop and time closed circuit.Using this principle, waved on weighing device according to got Culture bag quality judged, revolution speed is controlled by control device, if main culture bag quality is more than time culture bag quality, Then accelerate the speed of main circulation pump, dialysis direction flows to time closed circuit by main circulation loop, conversely, then accelerating time circulating pump Speed, dialysis direction main circulation loop is flowed to by secondary closed circuit.Compared by weighing, do not stop adjustment main circulation pump and follow with secondary The speed of ring pump, makes circuit system be finally reached balance or maintain unbalanced state.So as to reaching monitor in real time and adjusting in real time Whole.

If culture antibacterial, this utility model can be used for PDPK researchs, its workflow is：

1st, culture medium enters fluid path from storage culture bag, and after primary and secondary, circulation fluid path balance, primary and secondary closed circuit is set up.

2nd, the curve simulation stage：Curve simulation drug level uphill process, middle control unit are carried out according to target Drug-time curve Calculate, medicine is pumped at regular time and quantity in fluid path circulation, to reach aimed concn by administration unit；Curve simulation medicine declines In the stage, aimed concn is realized by calculating waste liquid exclusion amount and new plus culture medium liquid measure；After curve simulation is completed, cleaning Pipeline, prepares experiment next time；

3rd, sample collecting storage device timing acquiring sample is stored.Drug level in manual detection sample, in middle control The measured value for obtaining is input in device, and equipment draws actual measurement drug concentration time curve.

4th, enter into curve study stage, repeat above operate, in drug level ascent stage, middle control unit according to The drug level of curve simulation and the relation of liquid volume added, calculate in curve learning process, reach the medicinal liquid of this target Amount, adds medicinal liquid at regular time and quantity by administration unit；In the drug level decline stage of a curve study, middle control unit is calculated Go out the dependency relation of drug level and this aimed concn and last time concentration proportion, draw and reach what respective objects concentration should be extracted out Waste liquid and fresh culture liquid measure, timing is executed extracts waste liquid and injection fresh culture operation out.Sample collecting storage dress Put timing acquiring and store sample, manual detection goes out sample concentration.

If the Drug-time curve that 5 steps 4 are obtained and aim curve error still have a larger error, repeat step 4, until Each point tolerance is within 10%；After curve study, after fluid path environment is set up, manually antibacterial is injected with injector for medical purpose, Resistance experiment is carried out, by harvester in each sampling point sampling, analysis bacterial condition quantity and liquor strength, is painted Curve when Drug-time curve processed and bacterium.

Beneficial effect：

1st, this utility model is when the culture medium containing cell passes through hemodialyzer, cell and some biomacromolecules （Cytokine etc.）Material can be moved in the main circulation loop of hemodialyzer, and water, inorganic salt and small molecule metabolic waste Time closed circuit is entered etc. doughnut can be passed through.

2nd, this utility model is easy to operate, and precisely, expansibility and maintainability are relatively strong, can facilitate medical work for metering Person carries out the preparation for the treatment of immunologically competent cell.

3rd, the culture medium in the instrument is recycled by double circulation structure, and the waste and old culture medium in hemodialyzer can To be filtered, and the material such as cytokine is retained, and can effectively prevent the loss of the materials such as cytokine, is improved and is utilized Rate.And new culture medium can be continuously added as needed in main circulation loop, it is also possible to from secondary closed circuit constantly Discarded culture medium is detached, and the culture medium in main circulation loop is maintained to cell or the provisioning of bacterial growth.Additionally, cell training Foster process needs cytokine to be induced, and makes cell directional induction and efficient amplification in vitro, and passes through hemodialyzer not Cell depletion can be effectively prevented only, also cytokine can be effectively isolated, preventing which from flowing into waste liquid causes to consume in time circulation Damage, reduce cell culture cost.

4th, the culture medium in this utility model can be recycled, cost-effective, and according to pH value, the change of cell concentration Change so as to judge the growing state of cell and the utilization power of culture medium, supplement fresh culture at any time, monitor in real time reaches height Effect induction and the purpose of amplifying cells.

5th, in this utility model, main culture bag and secondary culture bag adopt closed culture, but the material of culture bag is ventilative Material is made, and can realize independently breathing freely, directly complete gas exchange with CO2 in incubator.Bag mouth is closed, and is internally formed aseptic Environment, reduces pollution, and culture bag shaped flat to the full extent, and gas exchange area is big, beneficial to big in a limited space Amount cultured cells.

6th, the most cells using this utility model culture are present in main culture bag, and convenient collection cell is carried out subsequently Operation, and preparation method is simple, step is few, reduces extraneous and cells contacting chance, and reducing pollution may.

7th, in this utility model, PH structure of the detecting device is simple, easy to use, need not take out culture medium can achieve closing Under environment in real-time detection pipeline culture fluid pH value.LED plays the effect of illumination, and color sensor can be helped accurately will The color feedback of each time period is processed to machine, so as to draw corresponding pH value.Keep LED and color sensor position In same horizontal line, light can be concentrated on the position gathered required for color sensor so that the R of collection, G, B value is more smart Really.Bracing frame is provided with track, and LED cover plate and color sensor cover plate are arranged on by slide block on the track of bracing frame, can To be applied to the pipeline drip chamber of different size, draw-in groove is set in order to fix between LED cover plate and color sensor cover plate Away from the design causes the scope that this utility model is suitable for wider.Color sensor is used for measuring pH, can realize monitor in real time, Sensitivity is high.

8th, the on-line sample acquisition and storage device in this utility model can realize automatic sampling, storage and collection pin Cleaning operation, makes simple acquisition operations, efficiency high and safety good.Washing cup surface is provided with silver-plated sheath, can reduce thin Bacterium pollutes, and keeps the gnotobasiss of Washing cup itself, and silver-plated with low cost, and effective time is long, lifts the cleaning effect of injection needle Really.Two-dimentional machinery arm can realize injection needle, every bottle stopper plate, the alignment of sample pore；When injection needle is moved downward, alignment insertion Sample cell aperture；After injection needle injection sample cell, injection needle is moved in Washing cup and is cleaned, can realize adding automatically Sample, and it is directed at automatically sample cell.Reset of the control device by optocoupler signal control motor, it is to avoid the piston of syringe is pressed onto Bottom.Automatization's sample collecting apparatus reduce production cost, and eliminate the problem of the high fault rate that labyrinth brings. Vacuum specimen tube and injection needle can be separated every bottle stopper plate, after preventing under subnormal ambient injection to be needled into vacuum specimen tube and table Between the rubber stopper of face, frictional force is larger, pull-up vacuum specimen tube.Hall element has the feature of stable performance, accurate positioning, Initial reset for sample box of the bottom with little magnetic piece.Especially in the case of 4 degrees Celsius of preservations of sample, can produce Condensed water, Hall element compare the original papers such as infrared sensor with the characteristic not affected by condensed water, stably can play Effect.Membrane pump can in severe working environment, during such as discharge of wastewater, due to sewage in impurity many and composition is multiple Miscellaneous, pipeline is easy to block, and so just produces the too high situation of load to electric pump, and the motor feels hot and is easily lost, and pneumatic diaphragm pump Granule and flow is adjustable can be passed through, be automatically stopped during line clogging to unobstructed.Level sensor can implementing monitoring, prevent from cleaning Liquid lacks causes the situation for cleaning failure and sample contamination to occur.And can monitor automatically, reduce and manually check cost and area Possibility of pollution in domain.Ultraviolet-sterilization is different from other sterilization modes, there is to air good sterilizing function, and safety is without dirt Dye, can control at any time, be arranged on beside injection needle and can avoid causing damage the sample in glass tube with vacuum.Sample cell Using vacuum specimen tube, internal is enclosed environment, can avoid polluting；Meanwhile, vacuum specimen tube manually need not close the lid, Both facilitated, the verity of exogenous influence sample can be prevented again.

9th, the rocking equipment of weighing in this utility model can adjust primary and secondary Cyclic culture base flow according to weighing results are waved Dynamic speed, so as to control the amount for increasing or abandoning culture medium, or the speed that culture medium is changed, reaches the purpose for automatically controlling, Manpower is saved, more precisely.The device ensure that oscillating motion gently carry out, stable performance, and wave while realizing weighing Function, can real-time monitoring culture medium weight, provide data support, and low cost for instrument controlling.Fixed column is connected with roller bearing Connect, play a part of to support roller bearing.The apparatus structure is simple, stable performance, and directly adopts sensor design, data directly to turn Change electrical signal, transmission is fast, more accurately, facilitates system-computed, carries out instrument controlling in time.Sensor stand is removable with balancing frame Connection is unloaded, is convenient for changing, maintenance cost is low.Balancing frame is provided with three equilibrium points, and 3 roller bearings are centrally formed triangle and triangle Balancing frame is engaged, more stable, and swing angle is little, waves evenly, can persistently relax and rock, and prevents cell attachment from giving birth to Long, while uniform mixed culture medium, provides the environment being suitable for cell or antibacterial etc..Pallet is slant setting, and inclination angle scope is 5 ° ~ 30 °, it is ensured that lowest part of the pipeline being connected with medium container in the process of rocking always situated in pallet, it is ensured that in pipeline Culture medium is filled.

Description of the drawings

Fig. 1 is this utility model structural representation；

Fig. 2 is this utility model system schematic；

Fig. 3 is the structural representation for waving weighing device in this utility model；

Fig. 4 is the side schematic view for waving weighing device in this utility model；

Fig. 5 is culture bag structural representation in this utility model；

Fig. 6 is the structural representation of sample collecting storage device in this utility model；

Fig. 7 is the front view of sample collecting apparatus in this utility model；

Fig. 8 is the rearview of sample collecting apparatus in this utility model；

Fig. 9 is the structural representation of sample storage device and cleaning device in this utility model；

Figure 10 is the structural representation of pH detection means in this utility model；

Figure 11 is the axonometric chart of pH detection means in this utility model；

Figure 12 is the schematic diagram of the color sensor of pH detection means in this utility model;

Reference：1st, main circulation loop；2nd, secondary closed circuit；3rd, main circulation pump；4th, secondary circulating pump；5th, doser； 6th, main culture bag；7th, secondary culture bag；8th, master waves weighing device；9th, secondary weighing device is waved；10th, culture bag is stored；11st, waste liquid Culture bag；12nd, hemodialyzer；13rd, sample collecting storage device；14th, pH detection means, 15, control device；16th, temperature control System processed；17th, major cycle fluid path pressure-detecting device；18th, secondary circulation fluid path pressure-detecting device；1-1, medicinal bag；1-2 drawing liquids Syringe pump；1-3 injecting liquid drug pumps；1-4 feed liquor syringe pumps；1-6 control boards；A, B are main circulation loop region, and C, D are secondary Closed circuit region；

8-1, pallet；8-2, LOAD CELLS；8-3, motor；8-4, sensor stand；8-5, roller bearing；8-6, fixed column； 8-7, motor connection axle；8-8, wave balancing frame；8-9, wave machine support；8-10, culture bag；8-11, culture bag hanging hole are fixed Post；8-12, the culture bag inlet pipe mouth of pipe；8-13, the culture bag outlet pipe mouth of pipe；8-14, culture bag hanging hole；

13-1, sample collecting apparatus, 13-2, sample storage device, 13-3, cleaning device, 13-4, mechanical injection device, 13-5, ultraviolet sterilization device；13-1-1, syringe, 13-1-2, sampling needle, 13-1-3, fixed plate, 13-1-4, push-and-pull are slided Block, 13-1-5, V-block, 13-1-6, depression bar molectron, 13-1-7, line slideway, 13-1-8, lead screw motor, 13-1-9, Feed screw nut's connecting plate, 13-1-10, optocoupler catch, 13-1-11, optocoupler, 13-1-12, tee T, 13-1-13, clamp Valve, 13-1-14, pipe clamp；13-1-15, injection pipeline；13-1-16 sample lines；13-1-17 cultivates liquid pipeline；

13-2-1, reducing motor, 13-2-2, bearing block, 13-2-3, rotation dish, 13-2-4, sample box, 13-2-5, Sample box support, 13-2-6, every bottle stopper plate, 13-2-7, Hall element, 13-2-8, sample cell；

13-3-1, Washing cup, 13-3-2, the first membrane pump, 13-3-3, the second membrane pump, 13-3-4, liquid level sensing Device, 13-3-5, connects liquid bottle, 13-3-6, waste liquid bottle；

13-4-1, mechanical arm, 13-4-2, pin folder, 13-4-3, injection needle；

14-1, LED；14-2, LED cover plate；14-3, color sensor；14-4, color sensor cover plate；14-5, Support；14-6, pipeline drip chamber；14-3-1, Red lightscreening plate；14-3-2, green color filter；14-3-3, blue color filter.

Specific embodiment

With reference to the accompanying drawings and examples this utility model is described in further detail.

Embodiment

As shown in Fig. 1 to 12, a kind of organism culturing device based on hemodialyzer, including hemodialyzer 12, circulation Pump, weighing device, sample collecting storage device 13, doser 5, culture bag 8-10 is waved, described culture bag includes main training Foster bag 6 and time culture bag 7, the inside two ends of 12 main body of described main culture bag 6 and hemodialyzer are connected to form major cycle and return Road 1, the outside two ends of 12 main body of described secondary culture bag 7 and hemodialyzer are connected to form time closed circuit 2, described circulation Pump includes main circulation pump 3 and time circulating pump 4, and described main circulation pump 3 is located at the pipe between main culture bag 6 and hemodialyzer 12 On road, on the pipeline time between culture bag 7 and hemodialyzer 12, described waving is weighed dress for described secondary circulating pump 4 Putting and weighing device 9 being waved including the main weighing device 8 that waves with secondary, described master waves weighing device 8 and is located under main culture bag 6 Side, the described secondary weighing device 9 that waves is located at the lower section of time culture bag 7, described sample collecting storage device 13, doser 5 are connected with the pipeline between main culture bag 6 and hemodialyzer 12.

Described hemodialyzer 12 is made up of capillary fiber pipe and shell, the inside of described 12 main body of hemodialyzer Refer to that the inside of capillary fiber pipe, the outside of described hemodialyzer 12 refer to the space between shell and capillary fiber pipe.

Described doser 5 includes that medicinal bag 1-1 and syringe pump i.e. injecting liquid drug pump 1-3, described medicinal bag pass through Pipeline between drug infusion pumps 1-3 and main culture bag 6 and hemodialyzer 12 is connected.

Described circulating pump is peristaltic pump.

The described weighing device that waves includes waving machine support 8-9, motor 8-3, waves balancing frame 8-8, pallet 8-1, title Sensor 8-4 is retransmitted, located at waving on machine support 8-9, the described balancing frame 8 that waves includes roller bearing 5 and balance to described motor 8-3 Disk, described roller bearing 5 are connected with balancing frame；Described motor 8-3 is connected with roller bearing 8-5 by motor connection axle 8-7；Institute Sensor stand 8-4 is provided between the balancing frame that states and pallet 8-1, is provided with LOAD CELLS in described sensor stand 8-4 8-2, described LOAD CELLS 8-2 are connected with pallet 8-1.

Described waving is additionally provided with fixed column 8-6 on machine support 8-9, described fixed column 8-6 is connected with roller bearing 8-5.

Described sensor stand 8-4 is detachably connected with balancing frame.Described balancing frame is triangle balancing frame.Described Roller bearing 8-5 is three, and one of them is connected with motor connection axle 8-7.Three described roller bearing 8-5 are placed in isosceles triangle, Highly consistent parallel with machine support 8-9 is waved.Described pallet 8-1 is slant setting, described range of tilt angles is 5 °- 30°.

It is additionally provided with culture bag 8-10 on described pallet 8-1, the culture bag inlet pipe mouth of pipe 8-12 of described culture bag 8-10 With culture bag outlet pipe mouth of pipe 8-13 located at the bottom of tilted-putted culture bag 8-10.Culture bag extension is provided with above culture bag 8-10 Hole 8-14, described culture bag hanging hole 8-14 are engaged with culture bag hanging hole fixed column 8-11 on machine.

Described sample collecting storage device includes sample collecting apparatus 13-1, sample storage device 13-2, cleaning device 13-3, mechanical injection device 13-4 and control device 15；Sample collecting apparatus 13-1 is connected with mechanical injection device 13-4, and will The sample liquid extracted in pipeline is injected in mechanical injection device 13-4；During cleaning device 13-3 is to mechanical injection device 13-4 Injection needle 13-4-3 carries out disinfection cleaning；Mechanical injection device 13-4 injects sample liquid in sample storage device 13-2；Described Control device 15 and harvester 13-1, sample storage device 13-2, cleaning device 13-3, mechanical injection device 13-4 phases Even.

Described mechanical injection device 13-4 includes mechanical arm 13-4-1, pin folder 13-4-2 and injection needle 13-4-3；Described Mechanical arm 13-4-1 13-4-2 pressed from both sides by pin be connected with injection needle 13-4-3, described mechanical arm 13-4-1 controls injection needle Motions of the 13-4-3 in space, described mechanical arm 13-4-1 are connected with control device 15.Mechanical arm 13-4-1 can be realized Injection needle 13-4-1, every bottle stopper plate 13-2-6, the alignment of sample pore；When injection needle 13-4-3 is moved downward, alignment insertion sample QC aperture；After injection needle 13-4-3 injection sample cell 13-2-8, injection needle 13-4-3 is moved to Washing cup 13-3-1 Middle cleaning.

Described sample collecting apparatus 13-1 includes syringe 13-1-1, sampling needle 13-1-2, fixed plate 13-1-3, push-and-pull Slide block 13-1-4, V-block 13-1-5, depression bar molectron 13-1-6, line slideway 13-1-7, lead screw motor 13-1-8, screw mandrel spiral shell Female connecting plate 13-1-9, optocoupler catch 13-1-10, optocoupler 13-1-11, tee T 13-1-12；Described syringe 13-1-1's Piston handle is fixed on line slideway 13-1-7 by push-and-pull slide block 13-1-4, and line slideway 13-1-7 is fixed on fixed plate The top of 13-1-3；On feed screw nut connecting plate 13-1-9, optocoupler 13-1-11 pacifies described optocoupler catch 13-1-10 It is mounted in the middle part of fixed plate 13-1-3；Control device 15 passes through the reset for judging optocoupler signal control motor, it is to avoid syringe 13- The piston of 1-1 is pressed onto bottom.

The staving of described syringe 13-1-1 is fixed on fixed plate by depression bar molectron 13-1-6, V-block 13-1-5 The bottom of 13-1-3；Described lead screw motor 13-1-8 is connected by feed screw nut's connecting plate 13-1-9 and push-and-pull slide block 13-1-4 Connect；Push-and-pull slide block 13-1-4 can be moved up and down in slide rail under the drive of lead screw motor 13-1-8, and then drives syringe 13- The pull-up of 1-1 and depression.Described lead screw motor 13-1-8 is connected with control device 15.Described syringe 13-1-1 leads to The injection needle 13-4-3 that tee T 13-1-12 is crossed with sampling needle 13-1-2, mechanical injection device 13-4 is connected.

Sample storage device 13-2 includes reducing motor 13-2-1, bearing block 13-2-2, rotation dish 13-2-3, sample Box 13-2-4, sample box support 13-2-5, every bottle stopper plate 13-2-6；Described sample box 13-2-4 is located at rotation dish 13-2-3 On；Described reducing motor 13-2-1 is connected with sample box support 13-2-5；Described bearing block 13-2-2 is fixed on sample box On support 13-2-5, described rotation dish 13-2-3 is connected with the screw mandrel of reducing motor 13-2-1, described every bottle stopper plate 13-2- 6 are fixed on sample box support 13-2-5, positioned at the surface at sample box 13-2-4 centers, described reducing motor 13-2-1 with Control device 15 is connected.When injection needle 13-4-3 is from during every the downward fluid injections of bottle stopper plate 13-2-6 to sample cell 13-2-8, can keep away Exempt from sample cell pipe cap to come off with the rising of injection needle.Described sample box 13-2-4 is provided with sample pore, for placing sample QC 13-2-8.Sample cell 13-2-8 is placed on sample pore, waits the injection of injection needle 13-4-3.

The cleaning device 13-3 includes Washing cup 13-3-1, membrane pump, level sensor 13-3-4, meets liquid bottle 13-3- 5th, waste liquid bottle 13-3-6；Described membrane pump includes the first membrane pump 13-3-2 and the second membrane pump 13-3-3, described first Membrane pump 13-3-2 extracts the cleanout fluid connect in liquid bottle 13-3-5, and the second described membrane pump 13-3-3 extracts Washing cup 13-3-1 In waste liquid into waste liquid bottle 13-3-6.Described level sensor 13-3-4 is located at and connects liquid bottle 13-3-5 sides, for monitoring Connect the cleanout fluid surplus in liquid bottle, the first described membrane pump 13-3-2 and the second membrane pump 13-3-3 with 15 phase of control device Even.While injection needle 13-4-3 moves into Washing cup 13-3-1, the first membrane pump 13-3-2 works so that cleanout fluid rinses note Penetrate pin 13-4-3.Meanwhile, the second membrane pump 13-3-3 works, and the waste liquid extracted in Washing cup 13-3-1 enters waste liquid bottle 13-3-6 In.When connecing the cleanout fluid in liquid bottle 13-3-5 and being not enough, level sensor 13-3-4 can monitor the remaining cleanout fluid of cleanout fluid Measure and be uploaded to control device 5 and send warning operation.Described Washing cup 13-3-1 electroplate sheaths.

Described tee T 13-1-12 is Y-shaped three-way pipe, and described tee T is filled with sampling needle 13-1-2, mechanical injection The pipeline for putting the injection needle 13-4-3 connections of 13-4 is provided with pinch valve 13-1-13；Pinch valve has two, respectively normally opened clamp Valve and often pass pinch valve.When syringe is pulled up, normally opened pinch valve is opened, and normally closed pinch valve is closed, and completes the extraction of sample liquid；Work as note When emitter is pushed, normally opened pinch valve is closed, and normally closed pinch valve is opened, completes sample liquid and be injected into sample storage device.Described threeway Pipe 13-1-12 is fixed by pinch valve 13-1-13, and pinch valve 13-1-13 is fixed on pipe clamp.

Also include Hall element 13-2-7, described Hall element 13-2-7 in described sample storage device 13-2 Located at the bottom of sample box 13-2-4, position on the lower side in the middle part of sample box support 13-2-5, and sample box 13-2-4 is fixed on Bottom be fixed with fritter Magnet, when sample collecting apparatus 13-1 electrification resets, control device 15 pass through Hall element 13- 2-7 judges the position of Magnet, completes the reset of sample box 13-2-4 original positions.

Described on-line sample harvester 13-1 also includes that ultraviolet sterilization device 13-5, described ultraviolet sterilization device set Side in injection needle 13-4-3.

Described organism culturing device also includes that PH detection means 14, described PH detection means 14 are located at main culture bag 6 And the pipeline between hemodialyzer 12；

Described PH detection means 14 includes LED 14-1, LED cover plate 14-2, color sensor 14-3, color sensing Device cover plate 14-4, bracing frame 14-5, described LED 14-1 are arranged on LED cover plate 14-2, described color sensor On color sensor cover plate 14-4, described LED cover plate 14-2 and color sensor cover plate 14-4 is relative to be set 14-3 Put and be arranged on bracing frame 14-5, described color sensor 14-3 is made up of three photoelectric sensors, three photoelectric sensings Red lightscreening plate 14-3-1, green color filter 14-3-2 and blue color filter 14-3-3 is respectively equipped with device.

Described LED 14-1 and color sensor 14-3 are located in same horizontal line；Set on described bracing frame 14-5 There are track, described LED cover plate 14-2 and color sensor cover plate 14-4 to be arranged on the track of bracing frame 14-5 by slide block On；Track on described bracing frame 14-5 is provided with draw-in groove.

Also include storing culture bag 10 and waste liquid culture bag 11, described storage culture bag 10 is feed liquor note by syringe pump Penetrate pump 1-4 to be connected with main culture bag 6, described waste liquid culture bag 11 is drawing liquid syringe pump 1-2 and secondary culture bag 7 by syringe pump It is connected.Described organism culturing device also includes control device 15；Described control device 15 is the control of organism culturing device Module, control device 15 are used for the entire work flow for controlling biological culture, set up including environment, run, stop control, and fluid path is built Vertical, operation, stopping control, wave the control of weighing device, collect culture base data, and sample collecting storage device 13 starts to adopt The operations such as sample, stopping sampling, storage.

Environment is set up includes dual temperature control system, CO2 concentration control systems and ultraviolet lamp disinfection bactericidal unit 16, described Dual temperature control system provides two sets of constant temperature systems for device, using refrigeration and heating equipment, by the temperature of sample collecting memory area At 0-4 degree Celsius, the temperature control of other device regions is at 25-37 degree Celsius for degree control.Ultraviolet lamp disinfection bactericidal unit I.e. ultraviolet sterilization device 13-5 provides exterminating bacterium function for package unit.

Described organism culturing device also includes that fluid path pressure-detecting device, described fluid path pressure-detecting device are used for supervising The pressure of liquid in control fluid path circulation, it is ensured that system fluid path is normally run.

Described main culture bag 6 and time culture bag 7 using the material of closed culture, main culture bag 6 and time culture bag 7 are Gas permeable material, described gas permeable material are EVA material or PE materials.

Described hemodialyzer 12 assumes vertical or heeling condition in operation.

A kind of described organism culturing device based on hemodialysis post is may be disposed in incubator box body.

Claims (14)

1. a kind of organism culturing device based on hemodialyzer, it is characterised in that including hemodialyzer, circulating pump, wave Weighing device, sample collecting storage device, doser, culture bag, described culture bag include main culture bag and time culture bag, The inside two ends of described main culture bag and hemodialyzer main body are connected to form main circulation loop, described secondary culture bag and blood The outside two ends of liquid dialyser main body are connected to form time closed circuit, and described circulating pump includes main circulation pump and time circulating pump, , on the pipeline between main culture bag and hemodialyzer, described secondary circulating pump is located at time culture bag for described main circulation pump On pipeline and hemodialyzer between, the described weighing device that waves includes that master waves weighing device and waves dress of weighing with secondary Put, described master waves weighing device located at the lower section of main culture bag, and the described secondary weighing device that waves is located at time culture bag Lower section, described sample collecting storage device, doser are connected with the pipeline between main culture bag and hemodialyzer.

2. a kind of organism culturing device based on hemodialyzer according to claim 1, it is characterised in that described gives Medicine device includes medicinal bag and syringe pump, and described medicinal bag is by the pipe between syringe pump and main culture bag and hemodialyzer Road is connected.

3. a kind of organism culturing device based on hemodialyzer according to claim 1, it is characterised in that described follows Ring pump is peristaltic pump.

4. a kind of organism culturing device based on hemodialyzer according to claim 1, it is characterised in that described shakes Pendulum weighing device includes waving machine support, motor, waves balancing frame, pallet, LOAD CELLS, and described motor is located at wobbler On support, the described balancing frame that waves includes that roller bearing and balancing frame, described roller bearing are connected with balancing frame；Described motor leads to Cross motor connection axle to be connected with roller bearing；Sensor stand, described sensor is provided between described balancing frame and pallet LOAD CELLS is provided with frame, and described LOAD CELLS is connected with pallet.

5. a kind of organism culturing device based on hemodialyzer according to claim 4, it is characterised in that

Fixed column be additionally provided with machine support in described is waved, and described fixed column is connected with roller bearing；Described sensor stand It is detachably connected with balancing frame；

Described balancing frame is provided with three equilibrium points；

Described roller bearing is three, and one of them is connected with motor connection axle；

Three described roller bearings are placed in isosceles triangle, highly identical and parallel with waving machine support；

Described pallet is slant setting, and described range of tilt angles is 5 ° -30 °；

Culture bag is additionally provided with described pallet, and the inlet/outlet pipe of described culture bag is located at the bottom of tilted-putted culture bag Portion.

6. a kind of organism culturing device based on hemodialyzer according to claim 1, it is characterised in that

Described sample collecting storage device includes that sample collecting apparatus, sample storage device, cleaning device, two-dimentional machinery are injected Device and control device；Sample collecting apparatus are connected with two-dimentional machinery injection device, and the sample liquid extracted in pipeline is injected Into two-dimentional machinery injection device；Cleaning device carries out disinfection to the injection needle in two-dimentional machinery injection device cleaning；Two-dimentional machine Tool injection device injects sample liquid in sample storage device；Described control device and harvester, sample storage device, clearly Cleaning device, two-dimentional machinery injection device are connected.

7. a kind of organism culturing device based on hemodialyzer according to claim 6, it is characterised in that

Described two-dimentional machinery injection device includes two-dimentional machinery arm, pin folder and injection needle；Described two-dimentional machinery arm passes through pin Folder is connected with injection needle, and described two-dimentional machinery arm controls motion of the injection needle in two-dimensional space, described two-dimentional machinery arm It is connected with control device；

The sample collecting apparatus include that syringe, sampling needle, fixed plate, push-and-pull slide block, V-block, depression bar molectron, straight line are led Rail, lead screw motor, feed screw nut's connecting plate, optocoupler catch, optocoupler, tee T；

The piston handle of described syringe is fixed on line slideway by push-and-pull slide block, and line slideway is fixed on fixed plate Top；

, on feed screw nut's connecting plate, optocoupler is installed in the middle part of fixed plate for described optocoupler catch；

The staving of described syringe is fixed on the bottom of fixed plate by depression bar molectron, V-block；

Described lead screw motor is connected with push-and-pull slide block by feed screw nut's connecting plate；

Described syringe is connected with the injection needle of sampling needle, two-dimentional machinery injection device by tee T；

Described lead screw motor is connected with control device；

The sample storage device include reducing motor, bearing block, rotation dish, sample box, every bottle stopper plate, sample box support；

Described sample box is in rotation dish；

Described reducing motor is connected with sample box support；

Described bearing block is fixed on sample box support；

Described rotation dish is connected with the screw mandrel of reducing motor；

Described reducing motor is connected with control device；

Described is fixed on sample box support every bottle stopper plate, positioned at the surface at sample box center；

The cleaning device includes Washing cup, membrane pump, level sensor, connects liquid bottle, waste liquid bottle；

Described membrane pump includes that the first membrane pump and the second membrane pump, the first described membrane pump extract the cleaning connect in liquid bottle Liquid, the waste liquid in described the second membrane pump extraction Washing cup is into waste liquid bottle；

The first described membrane pump and the second membrane pump are connected with control device；

Described level sensor connects the cleanout fluid surplus in liquid bottle located at liquid bottle side is connect for monitoring.

8. a kind of organism culturing device based on hemodialyzer according to claim 7, it is characterised in that

Described tee T is Y-shaped three-way pipe, and described tee T is connected with the injection needle of sampling needle, two-dimentional machinery injection device Pipeline be provided with pinch valve；Described tee T is fixed by pinch valve, and pinch valve is fixed on pipe clamp；Described sample box Sample pore is provided with, for placing sample cell；

Also include in described sample storage device that Hall element, described Hall element are located at the bottom of sample box, Gu Sample box mid-stent position on the lower side is scheduled on, and the bottom of sample box is fixed with fritter Magnet；

Described Washing cup electroplate sheath.

9. a kind of organism culturing device based on hemodialyzer according to claim 1, it is characterised in that described life Thing culture apparatuses also include pH detection means, and described pH detection means is located at the pipeline between main culture bag and hemodialyzer On pipeline above and/or between secondary culture bag and hemodialyzer；

Described pH detection means includes LED, LED cover plate, color sensor, color sensor cover plate, bracing frame, described LED on the LED cover plate, described color sensor on color sensor cover plate, described LED Cover plate and color sensor cover plate are oppositely arranged and are arranged on bracing frame, and described color sensor is by three photoelectric sensors Composition, is respectively equipped with Red lightscreening plate, green color filter and blue color filter on three photoelectric sensors.

10. a kind of organism culturing device based on hemodialyzer according to claim 9, it is characterised in that

Described LED and color sensor are located in same horizontal line；Described bracing frame is provided with track, described LED Light cover plate and color sensor cover plate are by slide block on the track of bracing frame；Track on described bracing frame is provided with card Groove.

11. a kind of organism culturing devices based on hemodialyzer according to claim 1, it is characterised in that also include Storage culture bag and waste liquid culture bag, described storage culture bag are connected with main culture bag by syringe pump, and described waste liquid is trained Foster bag is connected with secondary culture bag by syringe pump.

A kind of 12. organism culturing devices based on hemodialyzer according to any one of claim 1-11, its feature It is, described organism culturing device also includes control device；Described control device is the control module of organism culturing device.

A kind of 13. organism culturing devices based on hemodialyzer according to any one of claim 1-11, its feature It is, described main culture bag and time culture bag adopt closed culture, main culture bag and the material of secondary culture bag for ventilative material Material.

A kind of 14. organism culturing devices based on hemodialyzer according to any one of claim 1-11, its feature It is, described hemodialyzer assumes vertical or heeling condition in operation.