TWM615369U - Glue-surface microneedle patch fot pet - Google Patents

Abstract

The present invention is a pet-specific rubber-faced microneedle patch, which comprises: a base sheet with a plurality of needle posts on the base sheet, and a microneedle needle body is combined with the upper part of the needle post, wherein the microneedle needle body contains medicine, In this way, in the delivery of pet medicines for external use, dissolvable microneedles are installed on the moisturizing patch, which is beneficial to break through the epidermal stratum corneum, and can prevent pets from.

Description

Adhesive surface microneedle patch for pets

Method for administering pet medicine for external use

The transmission of the drug through the skin can ensure the accurate delivery of the drug to the affected area, the safety of the drug (non-oral), simple operation, save repeated medical treatment, overcome some poor gastrointestinal absorption drugs, and make it for chronic pain or long-term drug administration. The patch can also achieve a slow release function.

Drugs that come into contact with the skin can enter the body through three main routes: sweat glands, stratum corneum (10-30 cell thickness) and hair follicles (or sebaceous glands).

Among them, hair follicles and sweat glands have fine pores to the outside, and drugs can penetrate directly. There are water-retaining keratin proteins on the periphery of the keratinocyte layer. The intercellular substance is mainly composed of long-chain ceramides, free fatty acids, triglycerides, cholesterol, cholesterol sulfate and wax esters and a small amount of protein (with enzymes). Mainly, such as esterase).

A microneedle is a micron-level square array of small needles that can pierce the stratum corneum of the epidermis painlessly to achieve the purpose of drug delivery. Most of them are made into patches, which can be used for injections of small molecule drugs, proteins, DNA and vaccines (Prausnitz, 2004). Microneedles can be roughly divided into four forms: 1. Solid microneedle (solid microneedle), the use of microneedles to puncture the skin to create a rupture, the puncture is attached to the drug patch to be administered, so that it can penetrate the body ( McAllister et al., 2003); 2. Coated microneedle (coated microneedle), the drug to be administered is coated on the microneedle, and the drug penetrates into the body while piercing the skin (Kim, Quan, Compans, Kang, & Prausnitz , 2010); 3. Hollow microneedle (hollow microneedle), like a general injection needle, adopts the form of puncture perfusion. There are tiny pores in the needle body. After piercing the skin, the liquid medicine passes through the pores and enters the body (Wang, Cornwell, Hill, & Prausnitz, 2006); 4. Dissolvable microneedle (dissolvable microneedle), using water-soluble or biological Degradable polymer materials (water-soluble polymers PVP, PVA, etc.) are coated for drug administration. After piercing the skin, the needle body degrades and the drug is released. The soluble microneedle does not use a metal needle body, which can improve the safety of recycling and reduce the generation of medical waste. The material that dissolves the needle, if chitosan or poly lactic-co-glycolic acid (PLGA) is selected to dissolve slowly, the drug can be released slowly to achieve continuous administration. effect. Therefore, dissolving microneedles are currently the most important research and development direction in the research of microneedles (Chen, Huang, Lai, & Ling, 2013; Lee, Park, & Prausnitz, 2008). It can also be used on pets to prevent the application of the patch from falling off due to the scratching and biting of the patch.

Prepare micron-sized molds. Configure polydimethylsiloxane (PDMS), with 10:1 A (PDMS base material) and B agent (Curing agent) mixed with each other and placed in a shaking water tank to remove bubbles, pour into the stainless steel microneedle master mold (bottom size 300 x 300 microns; height 600 microns; array 9×9) and pin column structure master mold (bottom size) Inch 300 x 300 microns: height 800 microns; top size 130 x 130 microns; array 9×9), so that the PDMS completely covers the above-mentioned master mold. After the bubbles in the solution float out, the master mold can be placed Put it in a 90°C oven to cure and turn over the mold for about 30 minutes to prepare micron-level microneedle molds and pin column structure molds.

Needle column structure is prepared. Configure the needle column solution, take 5g PVP and 5g PVA into 10ml of deionized water (PVP: PVA: ddH2O = 1: 1: 2 (w/w)), mix well for use. Put 0.5g of the needle column solution, flatten the mold of the needle column structure, and place it in a centrifuge tube at 5100rpm; temperature 28°C; centrifugation for 30 minutes to fill the mold. After that, it was dried at room temperature for one day, and the needle column structure (Figure 1 02) was taken out for use.

The microneedle body structure is prepared. Prepare 13% by weight of chitosan hydrogel solution, mix it with an appropriate amount of injected drugs or vaccines, take 180mg of the mixed solution and spread on the microneedle mold, and fill the mold at 5100rpm, temperature 30℃, and centrifugation for 120 minutes. After centrifugation, place the centrifuge tube in a Swing rotor, and centrifuge again until the centrifugation is complete. Cover the chitosan hydrogel with a thin layer of PVP/PVA polymer solution (about 0.1g). The butyl glycan is deposited under the mold under pressure and dried at room temperature for one day, and then cut off with the blade of the frozen section. The excess polymer solution on the microneedle mold is left in the mold with only the chitosan needles.

The joint of the needle body and the needle post. Dip a small amount of PVP/PVA (50%(w/w)) solution on the top of the needle column structure array, and align the needle column structure array with the mold hole containing the chitosan microneedles (Figure 1 01) Gently press down to bond the two together, and dry them in an oven at 37°C for 20 minutes. Remove the mold to form a microneedle patch array (Figure 1 03).

1: Microneedle body

2: Needle column

3: Base piece

Figure 1. The needle column is first manufactured on the base sheet, and then the needle body is manufactured. When the needle column and the needle body are both manufactured, the needle column and the needle body are physically pressed by using the adhesive solution and the needle body Combined to form a microneedle patch.

In order to facilitate the description of the central idea of the present invention expressed in the column of the above-mentioned new content, specific examples are used to express it. As shown in Figure 1, the present invention provides a pet-specific rubber-faced microneedle patch. The needle column 2 is first manufactured on the base sheet 3, and then the needle body 1 is manufactured. After the needle column 2 and the needle body 1 When the manufacturing is completed, the needle column 2 and the needle body 1 are physically pressed by using the adhesive solution, so that the needle column 2 and the needle body 1 are combined to form a microneedle patch, and the needle body 1 contains drugs and moisturizing ingredients.

Using the above to make a microneedle patch containing 1% hyaluronic acid moisturizing ingredients, the test was conducted on 9 healthy dogs aged 2-6 years, and then divided into A, B, C3 groups, each Group 3 dogs, use the new type of moisturizing microneedle patch, general commercially available patch, and microneedle patch without moisturizing ingredients on the belly position. One patch is applied at 10 o'clock every day. During the period, the behavior and the patch of the dogs are observed. The state of the tablet was used, and the condition of the treated skin was observed at 6 pm for a total of 5 days.

The first day

The second day

Third day

Fourth day

Fifth day

Using the above to make a microneedle patch containing 1% hyaluronic acid moisturizing ingredients, test 9 healthy cats aged 2-6 years old, divided into 3 groups A, B, and C, each with 3 cats. Use the new type of moisturizing microneedle patch, general commercially available patch, and microneedle patch without moisturizing ingredients on the belly. One patch is applied every day at 10 o'clock. During the period, the behavior of the dog and the state of patch use are observed. The condition of the treated skin was observed at 6 pm for 5 days in total.

The first day

The second day

Third day

Fourth day

Fifth day

The above information can prove that the moisturizing microneedle patch of the present invention can prevent pets from scratching and biting for the delivery of external medicines for pets and achieve the effect of medicine administration.

1: Microneedle body

2: Needle column

3: Base piece

Claims (3)

A pet-specific rubber-faced microneedle patch comprises: a base sheet with a plurality of needle posts on the base sheet, and a microneedle needle body is combined above the needle posts, wherein the microneedle needle body contains medicine. The pet-specific rubber-faced microneedle patch according to claim 1, wherein the needle column and the microneedle body are made of soluble materials. The pet-specific rubber-faced microneedle patch according to claim 1, wherein the needle column and the microneedle needle system further contain moisturizing ingredients.

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