TWM613588U - Gene amplification apparatus - Google Patents

Abstract

A gene amplification device is suitable for gene amplification of at least one sample. The gene amplification device includes a bracket, a rotating bearing, a motor, and a first airflow generator. The rotating bearing is rotatably arranged on the bracket and rotates. The socket has a accommodating groove and an air flow channel. At least one part is placed in the accommodating groove, and the accommodating groove is located on the air flow channel or its extension path. , The first airflow generator is used for generating a first airflow, and the first airflow flows along the airflow channel and flows through the accommodating groove.

Description

Gene amplification equipment

This creation is about a gene amplification device, especially a gene amplification device with a rotating seat with an air flow channel design.

Polymerase chain reaction (Polymerase chain reaction, PCR) is the use of gene polymerase to carry out chain replication of genes. Among them, real-time quantitative polymerase chain reaction (Real-Time PCR, qPCR) can monitor the entire polymerase chain reaction in real time. The polymerase chain reaction mainly includes the temperature control part and the detection part. The temperature control part provides the thermal cycle temperature required for the polymerase chain reaction. The detection part uses a specific excitation light wavelength to cause the fluorescent reagent to release a fluorescent reaction, and then uses an optical sensor and a filter to capture and detect a specific wavelength. Performing polymerase chain reaction once can get about 2 times the polymerase chain reaction product, after performing N times, about 2 ^N polymerase chain reaction product can be obtained, and when the polymerase chain reaction product doubles, the fluorescence reaction gradually increases and accumulates Therefore, real-time quantitative polymerase chain reaction can monitor the temperature change and fluorescence change of the entire polymerase chain reaction in real time, record the cycle number and fluorescence intensity change value, and then quantitatively analyze the gene.

In the conventional technology, the test tube system storing the gene to be tested is placed on the test tube holder of the gene amplification device. The test tube holder has the heat conduction effect. The heater and the thermoelectric cooling chip (Thermoelectric Cooling Module, TEC) thermally contact the test tube holder to heat or cool the test tube through the test tube holder. The heat dissipation fins are in thermal contact with the heater and the thermoelectric cooling chip to discharge waste heat generated by the heater and the thermoelectric cooling chip. However, the size of the current heat dissipation fins may not be sufficient to support the demand for real-time temperature control, and is limited by the volume limitation of the gene amplification equipment, and it is difficult to greatly increase the size of the heat dissipation fins.

The gene amplification device disclosed in an embodiment of the invention is suitable for gene amplification of at least one sample. The gene amplification device includes a bracket, a rotating seat, a motor, and a first airflow generator. The rotating bearing seat is rotatably arranged on the bracket. The rotating bearing seat has an accommodating groove and an air flow channel. At least one sample is placed in the accommodating groove, and the accommodating groove is located on the air flow channel or its extension path. The motor is arranged on the bracket to drive the rotating bearing seat to rotate relative to the bracket. The first airflow generator is used for generating a first airflow, and the first airflow flows along the airflow channel and flows through the accommodating groove.

According to the gene amplification device of the above-mentioned embodiment, by directly setting the airflow channel through the place where the test tube (sample) is placed on the heat conducting plate of the rotating holder, the airflow can directly raise and lower the temperature of the test tube (sample), so that the gene The amplification equipment meets the needs of instant heating and cooling.

The above description of the content of this creation and the description of the following implementation manners are used to demonstrate and explain the principle of this creation, and to provide a further explanation of the scope of the patent application for this creation.

Please refer to Figure 1 and Figure 2. Fig. 1 is a three-dimensional schematic diagram showing the gene amplification device of the first embodiment of the invention. Fig. 2 is a schematic diagram showing the three-stage thermal cycle temperature required for the polymerase chain reaction of the gene amplification device of the first embodiment of the invention.

As shown in FIG. 1, the gene amplification device 1 of this creative embodiment is a real-time quantitative polymerase chain reaction (qPCR) device, which mainly includes a temperature control system, an optical system, and a rotating system. The temperature control system uses, for example, a Thermoelectric Cooling Module (TEC), heater, fan, or radiating fins for heating and cooling to achieve rapid temperature rise and fall. As shown in Figure 2, the temperature control system provides the three-stage thermal cycle temperature required for the polymerase chain reaction to the test tube containing the fluorescent reagent. Each polymerase chain reaction must include denaturation to 95°C, Annealing to 50°C to 60°C, and extension to 72°C. . The optical system captures values and quantitatively analyzes the fluorescent reaction excited by the fluorescent reagent in the test tube after each thermal cycle. The rotating system uses a motor to rotate the turntable of the temperature control system (in one embodiment, the turntable carries, for example, 16 test tubes), so that 16 test tubes containing fluorescent reagents can correspond to different positions of the optical system, using the specific optical system The wavelength of the excitation light causes the fluorescent reagent to release a fluorescent reaction, and then the photodiode in the optical system captures the fluorescence brightness and analyzes the final gene concentration. The above-mentioned temperature values can be adjusted according to different genes and different reagents.

The optical system has four optical devices, and each optical device includes an excitation filter, an emission filter, and a photodiode. In one embodiment, the above-mentioned optical device can be used to detect four specific fluorescent lights: green (excitation wavelength 494nm, emission wavelength 520nm), yellow (excitation wavelength 550nm, emission wavelength 570nm), orange (excitation wavelength 575nm, emission wavelength 602nm) ), red (excitation wavelength 646nm, emission wavelength 662nm).

As far as the details of the optical device are concerned, in the optical device, the light emitted by the monochromatic light-emitting diode (LED) passes through the excitation filter, and then is reflected by the dichroic filter. Mirror (Dichroic filter) can reflect short waves and make long waves penetrate), and illuminate upward on the bottom of the test tube containing fluorescent reagent. After the fluorescent reagent is excited, the emitted fluorescence passes through the spectroscopic filter and then through the emission filter. (Emission filter), after filtering out all the unwanted noise light sources, it is received by the photodiode, and after a series of light paths, the final changes in fluorescence characteristics are observed for analysis.

The motor of the rotating system directly rotates the temperature control system, so that the test tubes containing fluorescent reagents on the temperature control system can respectively correspond to the positions of the four optical devices for detection. In one embodiment, the rotation system may include a limit sensor, which uses a light blocking method to detect the initial point (Home) and the stopping point (End) of the motor rotation position, and through firmware and software Distinguish the test tube currently being tested.

Please refer to Figure 1 and Figures 3 to 7. Fig. 3 is a schematic cross-sectional view showing the gene amplification device of the first embodiment of the present creation. Fig. 4 is a three-dimensional schematic diagram showing the wind deflector of the first embodiment of the invention. Fig. 5 is a schematic cross-sectional view showing another cut plane of the gene amplification device of the first embodiment of the present creation. Fig. 6 is a partial exploded schematic diagram showing the gene amplification device of the first embodiment of the present creation. FIG. 7 is a three-dimensional schematic diagram showing the heat conducting plate body of the first embodiment of the present invention from another perspective.

As shown in FIG. 1 and FIG. 3, the gene amplification device 1 of this creative embodiment is suitable for gene amplification of at least one sample. The gene amplification device 1 includes a support 100, a support such as a rotating support 200, a motor 300, and a first airflow generator 400.

In an embodiment, the bracket 100 includes a bottom 110 and a supporting portion 120. The supporting portion 120 stands on the bottom 110.

As shown in FIGS. 3 to 6, the rotating socket 200 includes a rotating disk 210, a heating disk 220 and a rotating disk cover 230. The rotating disk 210 is made of, for example, an insulating material such as plastic, and can rotate about a rotation axis A of the rotating bearing 200 relative to the support 100, such as in the direction a. The rotating disk 210 has a plurality of through grooves 211. The through slots 211 are arranged in a ring around the rotation axis A of the rotating bearing 200.

The heating plate 220 is, for example, fixed to the rotating plate 210 and includes a heat conducting plate body 221 and a heater 222. The heat conducting plate 221 is used to carry a sample, such as a liquid to be amplified. The heater 222 is in thermal contact with the heat conducting plate body 221. When the heater 222 is activated, the heater 222 will heat the sample through the heat conducting plate 221. In an embodiment, the heater 222 can also be changed to a cooling chip, which is not used to limit the present invention.

The heat conducting plate body 221 of the heating plate 220 has a circular shape, for example, and has a plurality of through grooves 2211, and the through grooves 2211 are arranged in a ring around the rotation axis A of the rotating bearing 200. The piercing groove 2211 and the piercing groove 211 together form the accommodating groove C. In other words, the accommodating grooves C are arranged in a ring shape around the rotation axis A of the rotating socket 200. The accommodating tank C is used to place a test tube 2 containing a sample (not shown). The rotating disk 210 is used to prevent the test tube 2 from tilting, and the heating disk 220 is used to heat the test tube 2.

The heat conducting plate body 221 of the heating plate 220 has a plurality of heat dissipation fins 2212 on a side away from the rotating plate 210. The shape of the heat dissipation fins 2212 is, for example, a flat plate, and is arranged radially to separate a plurality of airflow channels 2213. The accommodating grooves C are respectively located on the air flow channel 2213 or its extension path. In other words, in the radial direction of the heat conducting plate body 221, the accommodating groove C is located inside or outside the air flow channel 2213. In this way, when there is air flow through the air flow channel 2213, the air flow through the air flow channel 2213 will flow along the side of the accommodating tank C, and will remove the waste heat around the accommodating tank C to face the accommodating tank C. The inner test tube 2 is cooled down.

In one embodiment, the heat conducting plate body 221 of the heating plate 220 may also have a hollow structure 2214, such as an opening. The hollow structure 2214 is located at a position where the heat-conducting plate body 221 does not have the through groove 2211, such as the center of the heat-conducting plate body 221, so that the heat energy received by the heat-conducting plate body 221 is concentratedly conducted to the through groove 2211 (accommodating groove C). However, the design of the hollow structure 2214 in this embodiment is not intended to limit the present creation. In other embodiments, the hollow structure 2214 may not be provided in the thermally conductive plate body.

The rotating disk cover 230 is detachably installed on the rotating disk 210 and covers the accommodating grooves C. When the rotating disk cover 230 is detached from the rotating disk 210, the user can put the test tube 2 into the accommodating slot C or take it out from the accommodating slot C. When the rotating disk cover 230 is installed on the rotating disk 210, the rotating disk cover 230 will press the test tube 2 to fix the test tube 2 in the accommodating groove C and apply pressure to make the test tube contact the heating disk 220.

In one embodiment, the number of accommodating grooves C is multiple, but it is not limited thereto. In other embodiments, the number of the accommodating groove C can also be only a single one.

In an embodiment, the rotating bearing 200 may further include a wind deflector 240. The wind shield 240 is located on a side of the heating disk 220 away from the rotating disk 210 and covers one side of the air flow channels 2213 to prevent the air flow in the air flow channels 2213 from leaking away from the rotating disk 210. In other words, the wind deflector 240 is located on one side of the heating plate 220 in the axial direction, so as to concentrate the air flow in the air flow channel 2213 toward the accommodating groove C in the radial direction. The windshield 240 has a central opening 241 and a plurality of through holes 242. The through holes 242 are arranged in a ring around the rotation axis A of the rotating bearing 200. These perforations 242 are respectively aligned with the accommodating grooves C, and these perforations 242 allow the detection light of the optical system 600 (refer to FIGS. 1 and 8) to pass through so that the optical system 600 can be placed in the test tube 2 of the accommodating groove C. Samples for testing.

In an embodiment, the windshield 240 has these perforations 242 for the detection light of the optical system 600 to irradiate the test tube 2, but it is not limited to this. In other embodiments, if the material of the windshield 240 has light-transmitting ability, the windshield 240 may not need to be provided with these perforations 242.

In one embodiment, the rotating socket 200 may further include a heat insulating member 250. The heat insulating member 250 is, for example, plastic and is interposed between the rotating disk 210 and the heating disk 220 (heater 222) to avoid heating the disk 220. The generated heat is dispersed to the rotating disk 210. In this way, the heating speed of the test tube 2 in the containing tank C by the heating plate 220 is increased.

In one embodiment, the shape of the heat conducting plate 221 is a circular plate as an example to facilitate the description of the positional relationship between the accommodating groove C, the wind deflector 240 and the air flow channel 2213 through the axial and radial directions, but not This is limited. In other embodiments, the shape of the heat conducting plate body 221 can also be changed to other geometric shapes, such as a square plate shape.

As shown in FIG. 3, the motor 300 has an output shaft 310, and the rotating disk 210 of the rotating bearing 200 is fixed to the output shaft 310 of the motor 300. When the motor 300 is started, the motor 300 will drive the rotating socket 200 to rotate relative to the bracket 100.

As shown in FIGS. 3 to 5, the heater 222 and the first airflow generator 400 constitute, for example, the aforementioned temperature control system. The first airflow generator 400 is, for example, a fan, and the first airflow F1 generated by the first airflow generator 400 is used to flow to these airflow channels 2213, so as to pass the first airflow F1 to the test tube 2 in the containing tank C. Cool down. In detail, the gene amplification device 1 of this embodiment may further include a wind deflector 500. The wind deflector 500 is between the rotating bearing 200 and the first airflow generator 400, and the first airflow generator 400 is closer to the bottom 110 than the wind deflector 500. The air guiding hood 500 has a guiding channel 510, an air inlet 520 and an air outlet 530. The air inlet 520 and the air outlet 530 are respectively connected to two different sides of the guiding channel 510. The first airflow generator 400 is located at the air inlet 520 of the wind deflector 500, and the air outlet 530 of the wind deflector 500 communicates with the air flow channel 2213 through the central opening 241 of the wind deflector 240. In this way, the first air flow F1 generated by the first air flow generator 400 will first flow vertically upward to the heating plate 220 through the guide flow channel 510, and then flow horizontally to the accommodating tank C through the air flow channel 2213. , To cool down the test tube 2 in the containing tank C.

Since the first air flow F1 generated by the first air flow generator 400 is directly blown to the test tube 2 in the accommodating tank C, the cooling effect of the test tube 2 is better.

As shown in FIG. 3, in this embodiment, the output shaft 310 of the motor 300 passes through the guide channel 510 of the wind deflector 500 and is connected to the rotating bearing 200. In this way, the rotating bearing 200, the motor 300 and the wind deflector 500 can be arranged densely in a limited space, so as to further reduce the volume of the gene amplification device 1. However, the output shaft 310 of the motor 300 passing through the guide channel 510 of the air guide 500 is not intended to limit the present invention. In other embodiments, the output shaft of the motor does not need to pass through the guide flow passage of the wind deflector.

In one embodiment, the above-mentioned rotating system uses the motor 300 as an example, but it is not limited to this. In other embodiments, the rotating system can also be changed to other components that can allow the rotating bearing 200 to rotate.

As shown in FIGS. 3 and 4, in this embodiment, the number of the air inlet 520 and the first airflow generator 400 is, for example, but not limited to four each. The first airflow generators 400 are respectively disposed at the air inlet 520 so that the first airflow F1 generated by the first airflow generators 400 flows to the test tube 2 at the containing tank C together. The purpose of using multiple first airflow generators 400 is that under the same flow output, a smaller size first airflow generator 400 can be selected, and generally speaking, the smaller size of the first airflow generator 400 is less noisy. small.

Please refer to FIG. 1 and FIG. 8. FIG. 8 is a partial exploded schematic diagram of the gene amplification device according to the first embodiment of the invention. In an embodiment, the gene amplification device 1 may further include an optical system 600, which is fixed to the support 100 and is located around the wind deflector 500.

In an embodiment, the gene amplification device 1 may further include a second fluid generator 700 installed on the support 100 and used to generate a second air flow F2 blowing toward the optical system 600. The second air flow F2 can cool down the entire interior of the gene amplification device 1.

Please refer to FIG. 1 and FIG. 3 again. In one embodiment, the gene amplification device 1 may further include a central control module 800. The central control module 800 is fixed on the bottom 110 of the bracket 100, and is connected to the heater 222, the motor 300, the first airflow generator 400, the optical system 600, and the second airflow generator 700 through wired or wireless communication (electrical), for example , To control the opening and closing of the heater 222, the motor 300, the first airflow generator 400, the optical system 600, and the second airflow generator 700.

Please refer to FIG. 9. FIG. 9 is a schematic flow chart of the gene amplification method of the first embodiment of the present creation, which is suitable for gene amplification of at least one sample, and includes the following steps. First, as shown in step S11, the aforementioned gene amplification device 1 is provided. Then, as shown in step S12, in a first heating step, the heat conducting plate 221 is raised to a first temperature by the temperature control system. Next, as shown in step S13, in a first constant temperature step, the thermally conductive plate 221 is maintained at the first temperature by the temperature control system. Then, as shown in step S14, in a first cooling step, the heat conducting plate 221 is cooled to a second temperature by the temperature control system. Then, as shown in step S15, in a detection step, the heat conducting plate 221 is maintained at the second temperature by the temperature control system. Then, as shown in step S16, in a second heating step, the heat conducting plate 221 is raised to a third temperature by the temperature control system. Then, as shown in step S17, in a second constant temperature step, the heat conducting plate 221 is maintained at the third temperature by the temperature control system. Then, as shown in step S18, in a third heating step, the heat conducting plate 221 is raised to the first temperature by the temperature control system. Through the above steps S11 to S18, the temperature control of the heat conducting plate 221 can be performed through the temperature control system. More precisely, the temperature control of the test tube 2 and the sample in the test tube 2 can be performed through the temperature control system.

Please refer to FIG. 10 and FIG. 11. FIG. 10 is a schematic diagram showing the time-temperature curve of the gene amplification device of the first embodiment of the present creation during the gene amplification procedure. Fig. 11 shows a specific embodiment of the gene amplification method of the first embodiment of the present creation. As shown in step S12, in a first heating step, the heat-conducting plate 221 is raised to a first temperature (95° C.) by the temperature control system, such as heating the heat-conducting plate 221 through the heater 222. Then, as shown in step S13, in a first constant temperature step, the thermally conductive plate 221 is maintained at a first temperature (95° C.) by a temperature control system. Next, as shown in step S14, in a first cooling step, the heat conducting plate 221 is cooled to a second temperature (50°C) by the temperature control system, as the air flow generated by the first air flow generator 400 will be The heat conducting plate 221 is cooled to the second temperature (50°C), thereby reducing the temperature of the test tube. Next, as shown in step S15, in a detection step, the heat conducting plate 221 is maintained at a second temperature (50°C, detection) by a temperature control system. Then, as shown in step S16, in a second heating step, the heat-conducting plate 221 is raised to a third temperature (72°C) by the temperature control system, such as heating the heat-conducting plate 221 through the heater 222 . Then, as shown in step S17, in a second constant temperature step, the thermally conductive plate 221 is maintained at a third temperature (72°C) by a temperature control system. Then, as shown in step S18, in a third heating step, the heat conducting plate 221 is raised to the first temperature (95° C.) by the temperature control system, such as heating the heat conducting plate 221 through the heater 222. Before reaching the number of cycles, by repeating the above steps S13 to S18, the effect of gene amplification can be obtained. After reaching the number of cycles, the program ends.

Please refer to FIG. 12. FIG. 12 is a three-dimensional schematic diagram of the thermally conductive plate body of the second embodiment of the present creation. In this embodiment, the shape of the heat dissipation fins 2212a of the heat conducting plate 221a is columnar, and the heat dissipation fins 2212a are divided into multiple groups, such as three heat dissipation fins 2212a as a group. The heat dissipation fins 2212a in each group are arranged along the radial direction of the heat conducting plate body 221a, and the heat dissipation fins 2212a of these groups are arranged in a ring shape. In addition, any two of the heat dissipation fins 2212a of each group are arranged adjacent to each other to form an air flow channel 2213a.

Please refer to FIG. 13, which is a three-dimensional schematic diagram of the thermally conductive plate body of the third embodiment of the present creation. In this embodiment, the shape of each heat dissipation fin 2212b of the thermally conductive plate body 221b is a curved plate and is arranged in a radial pattern. Any two adjacent heat dissipation fins 2212b are spaced apart to form an air flow channel 2213b.

Please refer to FIG. 14. FIG. 14 is a three-dimensional schematic diagram of a thermally conductive plate body according to the fourth embodiment of the present invention. In this embodiment, the heat conducting plate 221c is not provided with heat dissipation fins, that is to say, the air flow channel 2213c presents a complete ring shape.

The number of the above-mentioned first airflow generator 400 is multiple, but it is not limited thereto. Please refer to FIG. 15. FIG. 15 is a schematic plan view of the first airflow generator and the wind deflector according to the fifth embodiment of the invention. In this embodiment, the number of the first airflow generator 400d is single, and the shape of the wind deflector 500d is fine-tuned according to the number of the first airflow generator 400d.

The above-mentioned gene amplification device 1 adopts a rotating bearing 200 as the bearing system, but it is not limited to this. Please refer to FIGS. 3 and 16. FIG. 16 is a schematic plan view showing the first airflow generator, the wind deflector, and the translational bearing of the sixth embodiment of the present invention. In this embodiment, the translation bearing 200e and the first airflow generator 400e are respectively located on opposite sides of the wind deflector 500e. The translational bearing 200e is similar in function to the above-mentioned rotating bearing 200, both of which can be used to carry and heat test tubes. The only difference is that the rotating bearing 200 rotates relative to the support 100, while the translational bearing 200e translates relative to the support 100. . The airflow generated by the first airflow generator 400e is guided by the wind deflector 500e to flow to the test tube carried by the translational bearing 200e.

The number of the heater 222 is single and surrounds the hollow structure of the heat conducting plate body 221, but it is not limited to this. Please refer to FIG. 17, which is an exploded schematic diagram of the heating plate of the seventh embodiment of the invention. In this embodiment, the heating plate 220f includes a heat conducting plate body 221f and a plurality of heaters 222f. The heater 222f is in thermal contact with the heat-conducting plate 221f and surrounds the groove 2211f of the heat-conducting plate 221f to heat the place where the test tube is placed in a focused manner, or the temperature at each groove 2211f can be individually controlled to more accurately Individually/locally regulate the temperature of the test tube and the sample in the test tube.

In one embodiment, the heater 222f is, for example, an aluminum nitride ceramic heating piece, which has high thermal conductivity, high hardness, low thermal expansion coefficient, corrosion resistance, low dielectric loss, non-toxicity, and electrical insulation. effect.

The heater 222 is in thermal contact with the heat conducting plate 221, but it is not limited to this. Please refer to FIG. 18, which is a three-dimensional schematic diagram of the air deflector, the heater, and the first airflow generator of the eighth embodiment of the invention. In this embodiment, the heater 900g is, for example, a ceramic heater and is located on the airflow path of the first airflow generator 400g. In this embodiment, the number of first airflow generators 400g is four as an example, and the number of heaters 900g is two as an example, and two of the first airflow generators 400g are directly installed on the wind deflector 500g. And the other two first airflow generators 400g are installed in the wind deflector 500g through the second heater 900g. In this way, when the first airflow generator 400g with the heater 900g is operated, the airflow generated by the first airflow generator 400g will be heated by the heater 900g and then blown to the accommodating tank, so as to oppose the accommodating tank. The test tube is heated. Furthermore, when the heat-conducting plate body is to be heated up, the heater 900g and the two first airflow generators 400g equipped with the heater 900g are turned on, and the other two first airflow generators 400g are turned off. Conversely, when the heat conducting plate body is to be cooled down, the heater 900g and the two first airflow generator 400g equipped with the heater 900g are turned off, and the other two first airflow generators 400g are turned on. In one embodiment, when the temperature of the heat conducting plate body is to be lowered, the heater 900g can also be turned off, and the four first airflow generators 400g are all turned on, so that the cooling effect is better.

In one embodiment, the positions of the heater 900g and the first airflow generator 400g can be reversed, that is to say, the heater is installed in the air deflector through the first airflow generator.

According to the gene amplification device of the above-mentioned embodiment, by directly setting the airflow channel through the place where the test tube (sample) is placed on the heat-conducting plate of the rotating holder, the airflow can directly raise and lower the temperature of the test tube (sample) to allow the gene to expand. The increased equipment meets the needs of instant temperature rise and fall.

Furthermore, by arranging heat dissipation fins on the heat conducting plate body of the heating plate, the cooling speed of the test tube by the heat dissipation airflow is further improved.

In addition, by directly contacting the heater of the heating plate with the heat-conducting plate body, and the heat-conducting plate body has a hollow structure where the test tube is placed, the heat energy provided by the heater can be more concentratedly conducted to the place where the test tube is placed, so that the gene can be expanded. Adding equipment meets the demand for immediate temperature rise.

Although this creation is disclosed above in the foregoing embodiments, it is not intended to limit this creation. Anyone familiar with similar skills should be able to make some changes and modifications without departing from the spirit and scope of this creation. Therefore, this creation The scope of patent protection for creation shall be subject to the definition of the scope of patent application attached to this manual.

1: Gene amplification equipment 2: test tube 100: bracket 110: bottom 120: Support 200: Rotating bearing (bearing seat) 200e: translation bearing (bearing seat) 210: Rotating Disk 211: piercing 220, 220f: heating plate 221, 221a, 221b, 221c, 221f: thermally conductive plate 2211, 2211f: through slot 2212, 2212a, 2212b: heat sink fins 2213, 2213a, 2213b, 2213c: air flow channel 2214: hollow structure 222, 222f: heater 230: Rotating Disk Cover 240: windshield 241: central opening 242: Perforation 250: heat insulation 300: Motor 310: output shaft 400, 400d, 400e, 400g: the first airflow generator 500, 500d, 500e, 500g: wind hood 510: Guide runner 520: air inlet 530: air outlet 600: Optical system 700: second airflow generator 800: Central control module 900g: heater a: direction A: Rotation axis C: holding tank F1: First airflow F2: second airflow

Fig. 1 is a three-dimensional schematic diagram showing the gene amplification device of the first embodiment of the invention. Fig. 2 is a schematic diagram showing the three-stage thermal cycle temperature required for the polymerase chain reaction of the gene amplification device of the first embodiment of the invention. Fig. 3 is a schematic cross-sectional view showing the gene amplification device of the first embodiment of the present creation. Fig. 4 is a three-dimensional schematic diagram showing the wind deflector of the first embodiment of the invention. Fig. 5 is a schematic cross-sectional view showing another cut plane of the gene amplification device of the first embodiment of the present creation. Fig. 6 is a partial exploded schematic diagram showing the gene amplification device of the first embodiment of the present creation. FIG. 7 is a three-dimensional schematic diagram showing the heat conducting plate body of the first embodiment of the present invention from another perspective. Fig. 8 is a partial exploded schematic diagram showing the gene amplification device of the first embodiment of the invention. Figure 9 is a schematic diagram showing the flow of the gene amplification method of the first embodiment of the present creation Fig. 10 is a schematic diagram showing the time-temperature curve of the gene amplification procedure performed by the gene amplification device of the first embodiment of the invention. Fig. 11 shows a specific embodiment of the gene amplification method of the first embodiment of the present creation. FIG. 12 is a three-dimensional schematic diagram showing the heat conducting plate body of the second embodiment of the invention. FIG. 13 is a three-dimensional schematic diagram showing the thermally conductive plate body of the third embodiment of the present invention. FIG. 14 is a three-dimensional schematic diagram showing the heat conducting plate body of the fourth embodiment of the invention. Fig. 15 is a schematic plan view showing the first airflow generator and the wind deflector of the fifth embodiment of the invention. Fig. 16 is a schematic plan view showing the first airflow generator, the wind deflector and the translational bearing of the sixth embodiment of the invention. Fig. 17 is an exploded schematic diagram showing the heating plate of the seventh embodiment of the invention. FIG. 18 is a three-dimensional schematic diagram showing the air guide hood, the heater and the first airflow generator of the eighth embodiment of the invention.

1: Gene amplification equipment

2: test tube

100: bracket

110: bottom

120: Support

200: Rotating bearing

210: Rotating Disk

220: heating plate

221: heat conduction plate

230: Rotating Disk Cover

300: Motor

310: output shaft

400: The first airflow generator

500: Wind hood

510: Guide runner

520: air inlet

530: air outlet

600: Optical system

800: Central control module

a: direction

A: Rotation axis

C: holding tank

F1: First airflow

Claims (22)

A gene amplification device is suitable for gene amplification of at least one sample, and the gene amplification device includes: A bracket; a rotating bearing seat, rotatably arranged on the bracket, the rotating bearing seat has an accommodating groove and an air flow channel, the at least one copy is placed in the accommodating groove and the accommodating groove is located in the air flow On the channel or its extension path; a motor arranged on the bracket for driving the rotating bearing to rotate relative to the bracket; and a first airflow generator for generating a first airflow, the first airflow generator An air flow flows along the air flow channel and flows through the accommodating groove. The gene amplification device according to claim 1, wherein the rotating bearing has a plurality of air flow channels and includes a plurality of heat dissipation fins, the heat dissipation fins separate the air flow channels, and the accommodating slot is located On one of the air flow channels or its extension path, the first air flow generated by the first air flow generator is used to flow to the air flow channels. The gene amplification device according to claim 2, wherein each of the heat dissipation fins has a flat plate shape and is arranged radially. The gene amplification device according to claim 2, wherein each of the heat dissipation fins has a curved plate shape and is arranged radially. The gene amplification device according to claim 2, wherein each of the heat dissipation fins has a columnar shape and is arranged in a ring shape. The gene amplification device according to claim 1, further comprising a wind deflector, the wind deflector having a guiding channel, an air inlet and an air outlet, the air inlet and the air outlet are respectively connected to the The first airflow generator is located at the air inlet of the air guiding hood, and the air outlet of the air guiding hood is connected to the airflow channel, so that the first airflow generator generates The first airflow flows to the airflow channel through the guide channel. The gene amplification device according to claim 6, wherein the motor includes an output shaft, and the output shaft passes through the guide flow passage of the air deflector and is connected to the rotating bearing. The gene amplification device according to claim 6, further comprising a plurality of first airflow generators, the air guide hood has a plurality of air inlets, and the first airflow generators are respectively arranged at the air inlets. The gene amplification device according to claim 6, wherein the support includes a bottom and a supporting portion, the supporting portion stands on the bottom, and the wind deflector is interposed between the rotary bearing and the first airflow generator And the first airflow generator is closer to the bottom than the wind deflector. The gene amplification device according to claim 6, further comprising an optical system, the optical system is fixed to the bracket and located around the wind deflector. The gene amplification device according to claim 10, further comprising a second fluid generator installed on the support and used for generating a second air flow to the optical system. The gene amplification device according to claim 1, wherein the rotating socket includes a rotating disk, a heating disk, and a rotating disk cover, the motor includes an output shaft, and the rotating disk is fixed to the output shaft of the motor The heating plate is fixed on the rotating plate, the containing groove is located on the rotating plate and the heating plate, and the rotating plate cover is detachably mounted on the rotating plate and covers the containing groove. The gene amplification device according to claim 12, wherein the heating plate includes a heat-conducting plate body and a heater, and the heater heats the heat-conducting plate body. The gene amplification device according to claim 13, wherein the thermally conductive plate body has a plurality of through grooves, the heating plate includes a plurality of heaters, and the heaters surround the through grooves of the thermally conductive plate body. The gene amplification device according to claim 13, wherein the rotary bearing has a plurality of accommodating grooves and a plurality of air flow channels, the heat conducting plate of the heating plate includes a plurality of heat dissipation fins, and the heat dissipation fins The fins are located on the side of the heat-conducting disk body away from the rotating disk, the heat dissipation fins separate the air flow channels, and the accommodating grooves are respectively located on the air flow channels or their extension paths. The gene amplification device according to claim 12, wherein the rotating bearing further comprises a wind shield, the wind shield is located on a side of the heating disk away from the rotating disk, and is covered by the air flow channels One side. The gene amplification device according to claim 12, wherein the rotating bearing further includes a heat insulating member, the heat insulating member being interposed between the rotating disk and the heating disk. The gene amplification device according to claim 1, wherein the rotating socket has a plurality of accommodating grooves, and the accommodating grooves are arranged in a ring around a rotation axis of the rotating socket, and the at least one element is suitable It is placed in any of the accommodating grooves. The gene amplification device according to claim 1, further comprising a heater located on the flow path of the first airflow of the first airflow generator. A gene amplification device, including: A bracket; a bearing seat, arranged on the bracket, the bearing seat having an accommodating groove and an air flow channel, the accommodating groove is located on the air flow channel or its extension path; a wind deflector, the wind deflector It has an air inlet and an air outlet, the air outlet of the wind deflector is connected to the airflow channel; and a first airflow generator located at the air inlet of the wind deflector, and the first airflow generator is used to generate a The first air flow, the first air flow flows from the air inlet to the air outlet, flows to the air flow channel and flows through the accommodating groove. The gene amplification device according to claim 20, further comprising a heater disposed between the first airflow generator and the air guide hood, and the first airflow is heated by the heater and then flows through the container.置槽. The gene amplification device according to claim 20, further comprising a heater which is in thermal contact with the accommodating tank, and the heater comprises an aluminum nitride ceramic heating piece.

Images