M405266 五、新型說明： 【新型所屬之技術領域】 尤指一種用於儲存血 本創作關於一種儲存袋系統 液及其衍生物的儲存袋系統。 【先前技術】 血 血液儲存技術是人類醫療史上非常重要的進泰 液儲存技術的精進使得許多重大的手術及疾病的 成為可能，並進而延長人類壽命。近年來，針斜血、^ 特定細胞的研究也越來越深入’比如說，分離出血^ 特定的白血球以研究免疫的相關課題，而其中又以幹、 胞的運用最受到矚目。因此’除了常規血液儲存之外 血液中特定細胞及幹細胞的儲存也日益受到重視。 幹細胞是出現在高等生物體發育初期之具有自 再生及無限分化潛力的細胞。雖然成體生物中仍保有特 定種類的幹細胞，但其數量及分化能力皆不若發育初其 的幹細胞，這使得發育初期的幹細胞更為珍責，而其錯 存的方法也因此成為研究中非常關鍵的部分。腾帶血θ 取得人類幹細胞最為便利的方式，然而人的一生僅有 次機會可以取得自身的臍帶血，因此臍帶血的儲存須妥 善規劃以配合日後所需之檢驗及可能的運用。 血液樣本通常需要保存於低溫中，如液態氣 t)，而日後運用時，往往需要取得少量的血液以進行产 4 及至少一出口；及至少一儲存部其設有至少一内部單 %及至少—入口；其中，前述儲存部之入口係與前述前 外里邻之出口連結；其中，前述儲存袋系統進—步包含 、封條，使母一前述内部單元與前述前處理部彼此之 間液體不相連通。 ^車父佳地’前述儲存袋系統係用於儲存周邊血液、臍 帶血或細胞懸浮液。 ，較佳地’前述儲存袋系統進一步包含一密封條，其 7剐述前處理部之内部單元中進一步區隔出一個與前 述内邠單7L及前述儲存部之内部單元之間液體不相連 通的内部單元。 A知上所述’本創作之儲存袋H㈣前處理部與储 存=的-體性設計，使儲存的樣本自採集到完成儲存皆 m間中處理’減少污染的風險。此外，藉著儲存 設計，可_分批取得部分縣進行檢驗或運 的目的，而避免整體樣本的毀損。 【實施方式】 為種新穎的儲存㈣統，其可簡單劃分 為刖處理邛和儲存部兩個 刀，其中别述刖處理部設有 土 V Π 4早兀、至少一 邻^ 及至7一出口；前述儲存 又有至少一内部單元 入口；其中前述前處理 M405266 部之入口係用以導入儲存物，且前述儲存部之入口係與 前述前處理部之出口連結。 本創作所述之「連結」係指兩個以上的構件以物理 方式直接或間接結合，其包含液體得或不得於前述兩個 以上的構件之間流通的狀態。 本創作所述之「液體相連通」或「連通」係指液體 得以於前述兩個以上的構件之間流通。可行地，前述儲 存部之入口係與前述前處理部之出口係以液體相連通 鲁 的方式連結。須注意的是，在本說明書中，除非特別指 明，否則所謂「連結」或「連通」皆具有液體得以流通 的意涵。 完成前述「液體相連通」的方式不受限制，舉例來 說，前述儲存部之入口係與前述前處理部之出口之間的 液體相連通的方式，可簡單為前述入口與出口的延伸， 或藉由一連通管來完成。前述連通管可為一可拆解式連 鲁 通管，並可視情況使前述儲存部及前述前處理部連結或 分離。 在本創作的一個實施態樣中，進一步包含一密封 條，其可： 使前述儲存部之内部單元與另一前述儲存部之内 部單元之間不處於液體相連通的狀態； 使任一前述儲存部之内部單元與前述前處理部之 8 M405266 間不處於液體相連通的狀態；及/或 自前述前處理部之内部單元中 t οΰ 几甲進一步區隔出一個 與前述内部^及前述儲存部之 相連通的内部單元。 早-之間液體不M405266 V. New description: [New technical field] A storage bag system for storing a bag system liquid and its derivatives, especially for storing blood. [Prior Art] Blood Blood storage technology is a very important advance in the history of human medical science. The advancement of the liquid storage technology has made many major operations and diseases possible, and thus prolonged human life. In recent years, studies on needle-streaked blood and specific cells have become more and more intensive. For example, hemorrhagic ^ specific white blood cells have been isolated to study the related issues of immunity, and the use of stems and cells has attracted the most attention. Therefore, in addition to conventional blood storage, the storage of specific cells and stem cells in the blood has received increasing attention. Stem cells are cells that have the potential for self-regeneration and infinite differentiation in the early stages of development of higher organisms. Although adult species still retain specific types of stem cells, their numbers and differentiation ability are not as good as those of their early stem cells. This makes the early stage stem cells more cherished, and the method of their misplacement is therefore very research-intensive. The key part. It is the most convenient way to obtain human stem cells. However, people have only one chance to obtain their own cord blood in their lifetime. Therefore, the storage of cord blood should be properly planned to meet the needs of future tests and possible applications. Blood samples usually need to be stored in a low temperature, such as liquid gas t), and in the future, it is often necessary to obtain a small amount of blood for production 4 and at least one outlet; and at least one storage portion is provided with at least one internal single% and at least An inlet; wherein the inlet of the storage portion is coupled to the outlet of the front outer front; wherein the storage bag system further includes, seals, so that the liquid between the inner unit and the pretreatment portion is not Connected. ^车父佳地' The aforementioned storage bag system is used to store peripheral blood, cord blood or cell suspension. Preferably, the storage bag system further comprises a sealing strip, wherein the internal unit of the pre-processing unit is further separated from the internal unit 7L and the internal unit of the storage unit. Internal unit. A knows that the storage bag H (four) pre-processing part of the creation and the physical design of the storage = the storage of the sample from the collection to the completion of the storage process to reduce the risk of pollution. In addition, by means of the storage design, some counties can be obtained in batches for inspection or transportation purposes, while avoiding damage to the overall sample. [Embodiment] For a novel storage (four) system, it can be simply divided into two knives for processing 邛 and storage, wherein the 刖 processing unit is provided with soil V Π 4 early 兀, at least one adjacent ^ and to 7 one outlet The storage further includes at least one internal unit inlet; wherein the inlet of the pre-treatment M405266 is for introducing the storage, and the inlet of the storage portion is coupled to the outlet of the pre-treatment portion. "Linked" as used in this creation means that two or more members are physically or directly bonded in a state in which a liquid is or may not be circulated between two or more members. The term "liquid phase communication" or "communication" as used in the present application means that a liquid can flow between the two or more members. Preferably, the inlet of the storage portion is connected to the outlet of the pretreatment portion in a liquid-connected manner. It should be noted that in this specification, unless otherwise specified, the term "connected" or "connected" has the meaning of the liquid being circulated. The manner of completing the above-mentioned "liquid phase communication" is not limited. For example, the manner in which the inlet of the storage portion communicates with the liquid between the outlets of the pretreatment portion can be simply the extension of the inlet and the outlet, or It is done by a connecting pipe. The connecting pipe may be a detachable connecting pipe, and the storage portion and the pre-processing portion may be connected or separated as the case may be. In an embodiment of the present invention, further comprising a sealing strip, wherein: the inner unit of the storage portion and the inner unit of the other storage portion are not in a liquid communication state; a portion of the internal unit that is not in fluid communication with the pre-processing unit 8 M405266; and/or further from the internal unit of the pre-processing unit, a portion of the inner portion and the storage portion The internal unit connected to it. Early - no liquid between
前述前處理部剌於接收簡存賴本，以 放樣本及/或精必麵處理措施。以血液樣本為例，= 取得的血液樣本在進行冷;東儲存前，必縣與The pre-processing unit is configured to receive the sample and/or the necessary processing measures. Take a blood sample as an example, = the blood sample obtained is cold; before the east storage, Bixian and
劑，如5屬的甘油或D_混合。較佳地^述前處 理部至少設有兩個人σ，—個人口供導人樣本，另一個 入口則供導入所需試劑（如前述冷束保護劑），據此，便 可使取得的血液樣本於㈣空間巾完成處理，減少污染 的風險。前述前處理部可包含至少一個内部單元，其= 每-個内部單元設有至少一個前述出口，以供取得儲存 於其中的物質或與前述儲存部的人口連結。前述内部單 兀的設置方式可能是以一隔壁所分隔設立，或以一密封 條在原先存在的内部單元中進一步分隔設立。 月’J述儲存部是本創作之儲存袋系統中儲存物質的 基本單位。在本創作的一個實施態樣中，前述儲存部係 經一隔壁分隔為至少一個内部單元，以達到分批儲存/ 分批取用的目的。前述隔壁係經設計可供使用者簡易地 以工具(如，剪刀）將前述内部單元分離。 在本創作的一個實施態樣中，前述儲存部之内部單 9 M405266 元可能同時或分别以—個入 相連通的方式連結》前述錯存部的二前f理部以液趙 元設有一出口，以视&〜 芏夕―個前述内部單 在本創作的-==其:的物質。 内部單元； ^ 則述儲存部設有兩個 卜H);較佳地」㈣兩個内部單元的容積比值為 前述兩個内部單元的容積比值為5〜10。 構，或者。的型態可如同傳統血袋之出血口的結 祕通/舉例來說，可能係一彈性結構，其可供針筒 、，f入而不至於破壞整體儲存袋系統的結構。前述 入口及/或W述出σ於操作中可藉任何習知手段加以暫 時性地封閉或開啟。 本創作之倚存袋系統係用於儲存任何生物性物 質，舉例來說，如，周邊血液、臍帶血或細胞懸浮液。 本創作之儲存袋系統中所有構件的材質無需特別 限制’原則上所屬領域中習知適合於低溫（如約 -196〜-197°C)長期儲存的塑膠類材質皆可使用，包括， 但不限於.聚乙歸、乙稀_乙酸乙稀g旨共聚物、氟樹脂、 聚亞胺矽酮、ABS聚合物（丙烯腈/丁二烯/苯乙烯聚合 物）、聚碳酸酯或其組合。 以下實施例將搭配圖式以説明本創作之細節，須注 意的是，以下實施利僅用於示例式地說明本創作之特色 M405266 及優點，而不用於限制本創作之申請專利範圍。此外， 以下圖式僅示意本創作的各種可行的態樣，而不欲限制 本創作的型態。特別需要留意的是，圖式中所有構件的 比例及相對位置皆僅具有示範性的效果，而不一定等同 於實際上的情況。 實施例一：本創作之儲存袋系統一 請參第一圖，本實施例的儲存袋系統100包含一前 處理部110及三個儲存部120。 前述前處理部110設有一内部單元111，以及與其 連通的入口 112、113。前述内部單元111並連結一出口 114以與一個前述儲存部120連結。 前述儲存部120經一隔壁121分隔為一較大的内部 單元122及一較小的内部單元123。前述内部單元122 和123共同與一入口 124連通，而前述内部單元】22並 與一出口 125連通。前述内部單元122的容量為10毫 升，而前述内部單元123的容量為1毫升。 以其中一個儲存部120為例，前述前處理部110係 藉前述出口 1M與前述儲存部120的入口 124連結，使 儲存物得以自前述前處理部110流通到前述儲存部120。 前述儲存袋系統100係用於儲存臍帶血。在使用 中，可預先自入口 1Π將冷凍保護劑（DMSO)置入前述 11 Έ405266 内部單元111，再將欲儲存的臍帶血自入口 112導入， 使臍帶血與冷凍保護劑在前述内部單元111中均勻混 合。實務上操作時，可簡單將前述儲存袋系統100放置 於桌面上，並使前述儲存部120的水平高度高於前述前 處理部110的水平高度。據此，可使臍帶血和試劑妥善 的於前述前處理部110中混合，而暫時不流入前述儲存 部120中。 接著，於臍帶血與試劑混合之後，再使前述前處理 鲁 部110和前述儲存部120置於相同水平高度（較佳地，使 前述前處理部110的水平高度高於前述儲存部120的水 平高度），便可使臍帶血經前述出口 114流入入口 124， 並分別進入内部單元122、123。 最後，待前述内部單元122、123裝有所欲儲存的 臍帶血後，以熱封機沿著密封線130將儲存袋系統100 的袋體熱封而形成一密封條。藉著該密封條，前述儲存 參 部120的前述内部單元122、123之間將處於液體不得 相連通的狀態，同樣的前述内部單元122、123與前述 前處理部110之内部單元111之間也不會再有液體流 通。據此，可再沿著密封線130以破壞性手段，如剪刀， 將前述前處理部110與前述儲存部120分離，並將前述 儲存部120置於一卡匣中，以儲存於液態氮。 日後，若要使用儲存中的臍帶血以進行必要之檢 12 驗’可簡單地以破壞性手段，將前述内部單元123與前 述内部單元122分離，而單獨將内部單元123取出。如 此一來’並可避免在拿取部分血液的同時使其他儲存中 的臍帶血解沐及毀壞。 實施例二：本創作之儲存袋系統二 請參第二圖，本實施例之儲存袋系統2〇〇包含一前 處理部210以及五個儲存部220。 前述前處理部210設有一内部單元211及兩個入口 212、213。前述内部單元211並連結出口 214、215以 與一個前述儲存部220連結。前述前處理部210另設有 —出口 216。 前述儲存部220經一隔壁221分隔為一較大的内部 單元222及一較小的内部單元223，並設有入口 224和 225分別獨立與前述内部單元222和223連通。前述内 部單元222並與一出口 226連通。前述内部單元222的 谷量為5毫升，而前述内部單元223的容量為1毫升。 前述儲存袋系統200係用於儲存臍帶血。在使用 中’可預先自入口 213將冷凍保護劑(DMSO)置入前述 内部單元211，再將採集到的臍帶血自入口 212導入， 使腾帶血與冷凍保護劑在前述内部單元 211中均勻混 合。實務上操作時，可簡單將前述儲存袋系統200放置 於桌面上，並使前述儲存部220的水平高度高於前述前 處理部210的水平高纟。據此，可使臍帶血和試劑妥善 的於4述前處理部21〇中混合，而暫時不流入前述儲存 部220中。 接著，於臍帶血與試劑混合之後，再使前述前處理 部Z10和前述儲存部22〇置於相同水平高度（較佳地，使 二述刖處理部210的水平高度高於前述儲存部22〇的水 平同度）’便可使臍帶血經前述出口 214、215分別流入 内部單元222、223。 爾後，可沿著密封線230將儲存袋系統2〇〇的袋體 熱封而形成-密封條’藉著該密封條，前述儲存部22〇 的前述内部單元222、223之間將處於液體不得相連通 ，狀態’同樣的前述内部單元222、223與前述前處理 P 0之内。|5單A 211《間也不會再有液體流通。 此時，若前述前處理部21〇之内部單元211中尚有 腾帶^留存’可簡單調整儲存⑽、統2GG的角度，使留 存的腾帶血聚集於内部單元211中設有出〇 216的一 側接著，再沿著密封線23丨將儲存袋系統200的袋體 ”、、封形成一岔封條。藉著該密封條，可自原先的内部單 兀211中形成一獨立的内部單元217，而此内部單元217 便可成為一個簡易的儲存部。 最唆，可再沿著密封線230、231以破壞性手段， 14A mixture of glycerol or D_ such as 5 genera. Preferably, the pretreatment portion is provided with at least two human σ, a personal mouth donor sample, and the other inlet for introducing a desired reagent (such as the aforementioned cold beam protectant), whereby the obtained blood can be obtained. The sample is processed in (4) space towels to reduce the risk of contamination. The pre-treatment unit may comprise at least one internal unit, wherein each of the internal units is provided with at least one of the aforementioned outlets for obtaining a substance stored therein or for connection with a population of the aforementioned storage unit. The arrangement of the aforementioned internal single rafts may be set up by a partition wall or may be further separated by a seal strip in the originally existing internal unit. The monthly storage section is the basic unit for storing substances in the storage bag system of the present creation. In one embodiment of the present invention, the reservoir is separated into at least one internal unit by a partition to achieve batch storage/batch access. The aforementioned partition walls are designed to allow the user to easily separate the aforementioned internal units by means of tools such as scissors. In an embodiment of the present invention, the internal unit 9 M 405266 of the storage unit may be connected at the same time or separately in a connected manner. The second front portion of the faulty portion is provided with an outlet for the liquid Zhao Yuan.视 & ~ 芏 ― - a previously mentioned internal single in this creation -== its: substance. The internal unit; ^ is described in the storage portion is provided with two H); preferably (4) the volume ratio of the two internal units is the volume ratio of the two internal units is 5 to 10. Structure, or. The pattern may be like the secret of a bleeding port of a conventional blood bag/for example, it may be an elastic structure that can be used for the syringe, without damaging the structure of the overall storage bag system. The aforementioned inlets and/or Ws may be temporarily closed or opened by any conventional means during operation. The creative relying bag system is used to store any biological substance such as, for example, peripheral blood, cord blood or cell suspension. The material of all the components in the storage bag system of the present invention need not be particularly limited. In principle, plastic materials suitable for long-term storage at low temperatures (such as about -196 to -197 ° C) can be used, including, but not It is limited to Polyethylene, Ethylene-Ethyl Acetate Copolymer, fluororesin, polyiminone, ABS polymer (acrylonitrile/butadiene/styrene polymer), polycarbonate or a combination thereof. The following embodiments will be combined with the drawings to illustrate the details of the present invention. It should be noted that the following embodiments are merely illustrative of the features of the present invention and the advantages thereof, and are not intended to limit the scope of the patent application of the present invention. In addition, the following drawings are merely illustrative of various possible aspects of the present invention and are not intended to limit the scope of the present invention. It is important to note that the proportions and relative positions of all components in the drawing are only exemplary and not necessarily equivalent to the actual situation. Embodiment 1: The storage bag system of the present invention. Referring to the first figure, the storage bag system 100 of the present embodiment includes a front processing portion 110 and three storage portions 120. The pre-processing unit 110 is provided with an internal unit 111 and inlets 112, 113 communicating therewith. The inner unit 111 is coupled to an outlet 114 to be coupled to one of the storage portions 120. The storage unit 120 is partitioned by a partition wall 121 into a larger inner unit 122 and a smaller inner unit 123. The aforementioned internal units 122 and 123 are in common communication with an inlet 124, and the aforementioned internal unit 22 is in communication with an outlet 125. The aforementioned internal unit 122 has a capacity of 10 ml, and the aforementioned internal unit 123 has a capacity of 1 ml. Taking one of the storage units 120 as an example, the pretreatment unit 110 is coupled to the inlet 124 of the storage unit 120 via the outlet 1M, so that the stored matter can flow from the pretreatment unit 110 to the storage unit 120. The aforementioned storage bag system 100 is for storing cord blood. In use, the cryoprotectant (DMSO) may be placed in the aforementioned 11 Έ 405266 internal unit 111 from the inlet 1 , and the cord blood to be stored is introduced from the inlet 112 to make the cord blood and the cryoprotectant in the aforementioned internal unit 111. Mix evenly. In practice, the storage bag system 100 can be simply placed on a table top, and the level of the storage portion 120 can be higher than the level of the pre-treatment portion 110. According to this, the cord blood and the reagent can be properly mixed in the pretreatment unit 110, and the inside is not allowed to flow into the storage unit 120 at this time. Then, after the cord blood is mixed with the reagent, the pre-treatment lug 110 and the storage portion 120 are placed at the same level (preferably, the level of the pre-treatment portion 110 is higher than the level of the storage portion 120). The height allows the cord blood to flow into the inlet 124 through the aforementioned outlet 114 and into the internal units 122, 123, respectively. Finally, after the inner unit 122, 123 is loaded with the cord blood to be stored, the bag body of the storage bag system 100 is heat sealed along the sealing line 130 by a heat sealing machine to form a sealing strip. By the sealing strip, the internal units 122 and 123 of the storage unit 120 are in a state in which the liquid is not in communication, and the same internal units 122 and 123 and the internal unit 111 of the pretreatment unit 110 are also There will be no more liquid circulation. Accordingly, the pretreatment portion 110 can be separated from the reservoir portion 120 by a destructive means such as scissors along the seal line 130, and the reservoir portion 120 can be placed in a cassette for storage in liquid nitrogen. In the future, if the cord blood in storage is to be used for the necessary inspection, the internal unit 123 can be separated from the aforementioned internal unit 122 by a destructive means, and the internal unit 123 can be taken out separately. As a result, it can avoid the removal and destruction of other stored cord blood while taking part of the blood. Embodiment 2: The storage bag system 2 of the present invention Referring to the second figure, the storage bag system 2 of the embodiment includes a front processing unit 210 and five storage units 220. The pre-processing unit 210 is provided with an internal unit 211 and two inlets 212 and 213. The inner unit 211 is connected to the outlets 214 and 215 to be coupled to one of the storage portions 220. The pre-processing unit 210 is further provided with an outlet 216. The storage portion 220 is partitioned into a larger inner unit 222 and a smaller inner unit 223 via a partition wall 221, and inlets 224 and 225 are provided to communicate with the inner units 222 and 223, respectively. The aforementioned inner unit 222 is in communication with an outlet 226. The inner unit 222 has a grain size of 5 ml, and the aforementioned inner unit 223 has a capacity of 1 ml. The aforementioned storage bag system 200 is for storing cord blood. In use, a cryoprotectant (DMSO) can be placed in the internal unit 211 from the inlet 213 in advance, and the collected cord blood can be introduced from the inlet 212 to make the blood and the cryoprotectant uniform in the internal unit 211. mixing. In practice, the storage bag system 200 can be simply placed on the table top, and the level of the storage portion 220 is higher than the level of the pre-treatment portion 210. According to this, the cord blood and the reagent can be properly mixed in the pretreatment unit 21, and temporarily do not flow into the storage unit 220. Then, after the cord blood is mixed with the reagent, the pretreatment portion Z10 and the storage portion 22 are placed at the same level (preferably, the level of the second processing unit 210 is higher than the storage portion 22〇) The level of the same degree) can cause the cord blood to flow into the internal units 222, 223 through the aforementioned outlets 214, 215, respectively. Thereafter, the bag body of the storage bag system 2〇〇 can be heat-sealed along the sealing line 230 to form a sealing strip. By the sealing strip, the aforementioned internal units 222, 223 of the storage portion 22〇 are not allowed to be in a liquid state. In the same state, the same internal unit 222, 223 is in the same state as the pre-processing P 0 described above. |5 Single A 211 "There will be no liquid circulation between the two. At this time, if the internal unit 211 of the pre-processing unit 21 has a suffocating band, the angle of the storage (10) and the system 2GG can be easily adjusted, and the remaining tidal blood is collected in the internal unit 211, and the exit 216 is provided. Then, the bag body of the storage bag system 200 is further sealed along the sealing line 23 to form a seal. By means of the seal, a separate internal unit can be formed from the original internal unit 211. 217, and the internal unit 217 can be a simple storage unit. Finally, it can be destructive along the sealing lines 230, 231, 14