M322543 八、新型說明： 【新型所屬之技術領域] 本創作係有關於-種操作平台，尤指一種應用於醫 學檢驗及分子生物學之全自動磁珠操作平台。 【先前技術】 自動化的磁珠操作平台主要分為三個核心部分：一 是，體或磁珠的移動方H磁鐵吸引磁珠的結構， 二疋整個自動化流程的控制方式。 而移動方式分為兩t —種是直接移動整個反應的 «Ϊ並利用幫浦，透過滴管尖吸取與排出液體，使得 刺用二到充分的混合；而另一種是只移動磁珠， 透過料棒套的移動，造成磁珠擾動， =磁=體達到充分的混合。至於磁鐵吸引磁珠的結 構’目W有兩種方式可達到吸引的效果：一是在滴管尖 m:加上永久磁鐵或電磁鐵’將磁珠固定在滴管尖 r二:峑向二是利用一支永久磁鐵棒或電磁鐵棒，插入 =棒套内’將磁珠固定在磁鐵磁極端的游棒套之外 广上。而自動化流程的控制方式分為兩種：一 曰片内種是將程式寫人機器中的 曰日片内，由内部直接控制。 :般來說’㈣或磁珠的移財式與顧吸引磁 、…’各有市場與使用領域，但最常被 應，，是在於不人性化的操作介面， 私的控制方式。外部控制的方式 = 設計，可以開發出很人性化且功能強大的摔 外加主機的空間浪費與移動上的不方便性， 但 電腦的不穩定性和連結介面的使用壽命與單價，都= M322543 用者在往後的維修費用上，得付出相當大的成本。至於 内部控制的方式，雜是使用單一晶片的模組，可: 使用上的穩定度，但簡單的操作介面，並不人性化，曰且 在程式編輯上’只容許少許參數的衫，使其在應用上 有所限制，甚至無法與其他機械設備相互配合使用 習知的自動化磁珠操作平台仍存有缺失而有待改進。 :步==加熱設計’需加入人工的以 自動手臂做移位動作，3:工;3另外增設昂貴的 與操作時間。 “部增加整體機台的設傷成本 自動化磁珠操作平A的 作流程自動化自動化’不僅是需要整個操 更換速产。二ϊί執仃相，更f縮短批次反應間的 污染，-直無適當的台2免批次反應的交叉 有待改進。料操作平台仍存有上述各項缺失而 題，所採取執仃時間與批次反應間之速度的問 孔盤内裝有試劑’，2用：是使用已封膜之九十六孔盤， 上之封膜，即可使用.考=要在操作實驗中，撕開孔盤 快速地對操作办門、二是利用紫外光的殺菌功能，可 、進仃清理與殺菌，以避免污染下-批 6 M322543 次的待測物。 【新型内容】 本創作之目的在提供—種全自動磁珠操作平a m腦並配合最新^ ^作，面的人性化與自動控制的穩定性，且增加= 全自動磁珠操作;二 吏 明瞭且便於操作該平台以發揮易於 磁珠操作平台適用於醫檢單位與研發單位。王自動 台，另一目的在提供一種全自動磁珠操作平 後上；加熱條、導熱率佳之導熱塊及可任意前 實驗上的靈活度拌:ί卢使用者在操作 作平台之適用性Γ ^ 日廣本創作全自動磁珠操 磁鐵棒之全自動磁珠操作平台包含：電腦、 ，其特徵在於前述全自動磁珠操作平台進-滑移於前』導=:導ΐ塊以及紫外光燈，前述孔盤 用以τι前述ΐ自動磁式面板’ 述孔丄前，孔盤為-具有九十六孔槽之孔盤，前 :佳、位，每一個槽位具有八個孔槽。 二個前述導熱:。則述孔盤對應配置二個前述加熱條及 盤。車父佳地，前述加熱條及前述導熱塊用以加熱前述孔 7 M322543 之第及及前述導熱塊係加熱前述孔盤 置入，套入於前述獅套内，並 蚪，則述磁鐵棒可吸附位於前述孔盤 中的磁珠，使則述磁珠附著於前述鮮棒套之外壁上。 較佳地，前述磁鐵棒為永久性磁鐵。 較佳地，前述紫外光燈用於清理與殺菌。 較佳地’前述全自動磁珠操作平台進一步包含溫产 控制用以提供加熱所需的電源控制與溫度偵測。又 本創作之優點：1.工程電腦之穩定性 1己憶=:= 讓使用者有更自由的編輯空間，以編輯出忿=化 ==更配工程電腦:視窗晝 m便插作，，面更佳人性化，淺顯易懂 與直接地觸控操作，讓使用者更 導槽之間，以方便置人與取出孔盤，^丨^地滑移於 以及在操作過程中，孔射保持固^孔盤到定位後 的，。4.經由加熱條與導熱塊的加熱：以== J:平台能夠更廣泛地應用於各種生化或二=作 ，、中，加熱條與導熱塊易於組裝與拆却。5.;=光 M322543 燈用於清理、殺菌磁珠操作平台内的操作封閉空間， 進而避免批次與批次之間的交叉污染。 【實施方式】 ’、 、雖然本創作將參閱含有本創作較佳實施例之所附圖 式予以充分描述，但在此描述之前應瞭解熟悉本行之人 ，可修改本文中所描述之創作，同時獲致本創作之功 f、。因此、，須瞭解以下之描述對熟悉本行技藝之人士而 3為^廣泛之揭示，且其内容不在於限制本創作。 、^參閱第一圖及第二圖，係分別顯示本創作全自動 磁珠操作平台1之立體圖及局部示意圖。本創作全自動 磁珠操作平纟卜包含電腦2、磁鐵棒3、授拌棒套4、 =珠5、、孔盤6以及導槽7，該電腦2具有—觸控式面板 ，用以控制該全自動磁珠操作平台丨的運作。 在參閱第—圖及第二圖’並配合參閱第三圖， 創作全自動磁珠操作平台1之容置㈣内之結 Π隹該ΐ自Λ磁珠操作平台1下部具有-容置空 f曰:13,且進一步包含加熱條8、導熱塊9以及紫外光燈 其二 =空=以容納該磁鐵棒3、該·棒 #4該孔盤6、该導槽7、加熱條8、導 外光燈10，而該磁鐵棒3、該攪拌棒套4、該孔般= 導熱塊9、該加熱條§係由上而下依序 ^ 水平方向地滑移於=二6 4内:該套可右議套 盤6中，該紫外光燈10可設於該容置動並，入於該孔 壁、右側壁、後側壁或上侧壁。 二3内之左側 M322543 • 請繼續參閱第—圖及第四圖’係顯示觸控式面板u 之示意圖。本，作全自動磁珠操作平台！除了電源開關 與溫度設定係藉由模組化之溫度控制器12直接切換控制 外，其餘功能皆由該觸控式面板u來控制。當開啟電源 後’電腦螢幕出現開機晝面，輕觸該觸控式面板u進入 ^面’，示主要的功能頁面’目前設計有：A.程式編 輯、B·程式執行、C·溫度控制與D·紫外光。 A.程式編輯：目前的編輯功能有「槽位選擇」、「混 φ合時間」、:磁吸時間」、「加溫時間」、「磁棒」的使用與 否、混合速度」、「溶液體積」與「暫停」等參數控制， 可視情況需要而加以增減。 Β士程式執行：會出現「目前執行的動作」，並顯示執 π’除此之外，還有「暫停功能」*「 的停止功能。 C. 溫度控制，可個別控制四組溫度控制器，並有風 扇與蜂鳴器的控制開關。 D. 紫外光，可控制該紫外光燈10的開關。使用者只 2輕觸朗控式面板U，並循著視窗的功能選項即可 上手、’而參數的更改，除了簡單的上下鍵選擇外， 英文字母視窗供參考’使用者不需經由 繁貞的教以1丨練’即可熟悉整個自動化流程的操作。 u孔皿6共有十二個槽位(如第三圖所示，該孔盤6 一左至右分別為槽位一、槽位二、槽位三……至槽位十 :)’而每-個槽位具有八個孔槽，一組八支磁鐵；3配 曰一組八支攪拌棒套4可插入於該孔盤6中的任—個槽 位之中的孔槽H圖觀之’每—個孔盤6能配合二 M322543 組共十六支的該磁鐵棒3與十六支的該攪拌棒套4，故可 在一個孔盤6上同時操作十六個檢體，而一個檢體有六 個槽位可供使用。本創作全自動磁珠操作平台〖係可配 置二個孔盤6,故在同一時間内，可處理的最大檢 三十二個。 以下較佳實施例係用於詳細說明本創作之操作方 式，並非用以限定本創作之範圍。 實施例一 ·· • 該孔盤6的六個槽位使用，以核酸的純化為例，其 配置方式如下·槽位一，裂解液的產物；槽位二，第一 種清洗液；槽位三，第二種清洗液；槽位四，第三種清 洗液；槽位五，磁珠與保存液；槽位六，沖提液。 首先使該攪拌棒套4先回原點，作原點校正。 第一步驟：移動該攪拌棒套4至槽位一中，並使該 攪拌棒套4上下快速地移動，以造成槽位一中的液體產 生流動，俾使細胞在裂解液中充分裂解，以釋放出核酸 或蛋白質。 • 第二個步驟··移動該攪拌棒套4至槽位五，且上下 快速地移動以分散槽位五中的該磁珠5，接著該磁鐵棒3 往下移動而插入該授拌棒套4内，使該磁珠5因磁力吸 附而附著於該攪拌棒套4前端的外壁上。 第三個步驟：同時移動該磁鐵棒3與該攪拌棒套4 至槽位二，再使該磁鐵棒3升起，此時，該 5註 攪拌棒套4前端的外壁脫落，該攪拌棒套4在第一種g 洗液中上下快速地移動，該磁珠5與第一種清洗液充分 混合，使該磁珠5表面達到清洗的效果，接著該磁鐵棒3 11 M322543 4前 再度往下移動，使該磁珠5再度吸附於該攪拌 端的外壁上。 第四個步驟：同時移動該磁鐵棒3與該攪拌棒套4 至槽位一，再使該磁鐵棒3升起，此時，該磁珠5自該 攪拌棒套4前端的外壁脫落，該攪拌棒套4在裂解液； 上下快速地移動，該磁珠5與裂驗充分混合，使該磁 珠5與核酸或蛋白質形成複合物，而吸附在—起 ，，鐵棒3再度往下移動，使該吸附與核酸或蛋白質形 .^複合物之磁珠5再度吸附於賴拌棒套4前端的外壁 第五個步驟：同時移動該磁鐵棒3與該授拌棒套, 位一’再使該磁鐵棒3升起，此時，該磁珠 攪拌棒套4前端的外壁脫落，該㈣棒套4在第 = =中上下絲地移動，該磁珠5彻_種清洗液^ =，使該磁珠5在第—種清洗液中達到最佳 果，去除不吸附的污染物，接著該磁鐵棒3再度 動’巧該磁珠5再度吸附於該攪拌棒套4前端的^卜。 第六個步驟：同時移動該磁鐵棒3 = ° ;槽位三，磁鐵棒3升起，此時:、;n； 攪拌棒套4刖端的外壁脫落，該攪拌棒套4 ^ 洗液中上下快速地移動，該磁珠5與第二種清洗二 Ϊ合丰ί該磁珠5在第二種清洗液中達到最佳的清洗^ 果，去除不吸附的污染物，接著該磁鐵棒3 政 動’ $該磁珠5再度吸附於該攪拌棒套4前端的；辟移 第七個步驟：㈣移動該磁鐵棒3與_拌 槽位四’再使該磁鐵棒3升起，此時，該磁珠 12 M322543 攪拌棒套4前端的外壁脫落，該攪拌棒套4在第三種产 洗液中上下快速地移動，該磁珠5與第三種清洗液充= 混合’使該磁珠5在第三種清洗液中達到最佳的清洗二 果，去除不吸附的污染物，接著該磁鐵棒3再度往下移 動，使該磁珠5再度吸附於該攪拌棒套4前端的^卜壁上。 第八個步驟：同時移動該磁鐵棒3與該獅才^套4 至槽位六，再使該磁鐵棒3升起，此時，該磁珠5㈣ 授拌棒套4前端的外壁絲，該㈣棒套4在沖提液°中 上下快速地移動，該磁珠5與沖提液充分混合，使該磁 珠5+在沖提液巾達到最麵沖提效果，將減沖提出來， 接者該磁鐵棒3再度往下移動，使該磁珠5再度吸附於 端的外壁上’而此乾淨的沖提產物，即 為純化後的核酸。 第九個步驟·同時移動該磁鐵棒3與該 二=:再使該磁鐵棒3升起，此時，該磁珠5自該 攪拌棒套4 W端的外壁脫落，該游棒套4在保存液中 ίΐΐΐ地移動’該磁珠5與保存液充分混合，使該磁 珠釋放純麵巾，接著賴轉套4往上移動，並 回歸至原點，程式終止。 閱第四圖’為了更符合實驗的參數要求， 本創作全自動磁珠操作平台丨的設計加 62預設處設有複數個條狀的加熱條二用以-次 二共八個孔槽)’且在該孔盤6與該加熱條8 之間=有V熱塊9’用以加熱效果更為均勻且快速，在 此:實施例中，該加熱條8與該導熱塊 對應於該孔盤6之槽位六及槽位十二。之又置处料 13 M322543 的轉作ί創作全自祕珠操作平台卜批次與批次 罢，而姑/丨、、°·利用該紫外光燈10以達到迅速滅菌的效 ίο的門關夕>Ύ染物的干擾；使用者藉由控制該紫外光燈 紫夕Γ#^’η可自由控制該紫外紐10滅菌之時間。另該 訾外朵二1Π具有兩種保護措施··一、當機門開啟時，該 i 會1行斷電，待機門關上後，該紫外光燈1〇 Α。二t電而點亮，用以避免於機門開啟時，紫外光外 饬处，羶Γ上的透明塑膠門片，則係具有吸收紫外光的 爲Ϊ冰忠肪用者可隨時觀察平台操作的情形，而無須擔 目1你田去Γ射的危險。二、若未將該紫外光燈10關掉， 則使用者無法執行自動化的操作程式。 實施例+ : 本創作全自動磁珠操作平台 1的使用，以100 μΐ的 王血)=，其整個的操作流程如下所述： 將，、將全血經高速離心之後，取其上層透明無色的血 水並使用已分注試劑的該孔盤6，撕開封膜，將血漿置 入槽位一，打開該全自動磁珠操作平台1的電源，出現 開機晝面’輕觸該觸控式面板11進入主頁面，進入溫度 f制丄將該加熱條8設成使綠態，並將溫度控制器的 μ度叹為65 C，然後進入程式控制，選擇程式，並執行 程，二即可進入執行程式的頁面，輕觸該觸控式面板11 之'复歸鍵’該㈣棒套4自動回到原點；將已裳入檢體 的β亥孔疏6與置於該孔盤6底下的導熱塊9 一起水平方 向推入該導槽7,並且平推到底，並輕觸執行鍵，使該全 自動磁珠操作平台1自動開始運作。首先使槽位一内的 M322543 吸:二酸’然後利用該磁鐵棒3與該攪 附槽位五中的該磁珠5，並移至槽位一 1=及接Λ依照次序分別在槽位二、槽位-、槽: —及槽位四進行清洗，其進行方式同前述 :加』：Ϊ步驟所述;待清洗完成後，經由該導熱塊9、 :绅；、二之加熱作用’對該孔盤6之槽位六進行加埶， ==釋放出來，然後再將該磁珠5移回槽位;、， ”錢拌棒套4亦回歸原點，便可結束程式。 可本創作提供—種全自動磁珠操作平台1， 化之it /貝夕達36種檢體，且可同時加溫、殺菌及電腦 動操作，提供使用者一整合性之磁珠操作平 口，有效解決傳統磁珠操作平台之缺失。 计-人ίϊΐ說明本創作的較佳實施例之後，熟悉該項技 楚的瞭解，在不脫離下述申請專利範圍與精 行各種變化與改變，且本創作亦不受限於說明 書中所舉實施例的實施方式。 15 M322543 【圖式簡單說明】 第一圖係顯示本創作全自動磁珠操作平台之立體 圖。 第二圖係顯示本創作全自動磁珠操作平台之局部 示意圖。 第三圖係顯示本創作全自動磁珠操作平台之容置 空間内之結構不意圖。 第四圖係顯示觸控式面板之示意圖。 【主要元件符號說明】 1…全自動磁珠操作平台 2— 電腦 3— 磁鐵棒 4…攪拌棒套 5…磁珠 6— 孔盤 7— 導槽 8…加熱條 9— 導熱塊 10- —紫外光燈 11 —觸控式面板 12— 溫度控制器 13- —容置空間 16M322543 VIII. New Description: [New Technology Field] This creation department has a kind of operation platform, especially a fully automatic magnetic bead operation platform for medical inspection and molecular biology. [Prior Art] The automated magnetic bead operating platform is mainly divided into three core parts: one is that the moving body of the body or the magnetic bead attracts the structure of the magnetic bead, and the control method of the entire automated process. The movement method is divided into two t-types, which are to directly move the whole reaction «Ϊ and use the pump to suck and discharge the liquid through the tip of the pipette, so that the thorn is used for two to fully mix; and the other is to move only the magnetic beads, through The movement of the rod sleeve causes the magnetic beads to perturb, and the magnetic = body reaches a sufficient mixing. As for the structure of the magnet attracting the magnetic beads, there are two ways to achieve the attraction effect: one is at the tip of the dropper m: plus a permanent magnet or an electromagnet 'fix the magnetic beads at the tip of the dropper r: two to two It is to use a permanent magnet bar or electromagnet rod, insert = rod inside the rod to fix the magnetic beads outside the rod sleeve of the magnetic pole of the magnet. There are two types of control methods for automated processes: one is to write the program into the next day in the machine, and it is directly controlled internally. : Generally speaking, (4) or the magnetic transfer of magnetic beads and attracting magnets, ...' each has a market and a field of use, but most often, it is an inhumanized operation interface, a private control method. External control mode = design, can develop a very user-friendly and powerful space waste and mobile inconvenience, but the instability of the computer and the service life and unit price of the interface are = M322543 In the future maintenance costs, the company has to pay a considerable cost. As for the internal control method, the module is a single chip, which can be used: the stability of the use, but the simple operation interface is not user-friendly, and the program editor only allows only a few parameters of the shirt, so that There are limitations in application, and even the inability to interact with other mechanical devices. There is still a need for improvement in the use of conventional automated bead operating platforms. : Step == heating design 'to be added to the manual to automatically shift the arm, 3: work; 3 additional expensive and operating time. “The ministry increases the cost of injury to the overall machine. The automatic automation of the magnetic bead operation level A is not only the need to replace the fast-moving production. The second phase is to suppress the pollution between batch reactions. The crossover of the appropriate batch 2 batch-free reaction needs to be improved. The material operation platform still has the above-mentioned missing items, and the reagent plate for the speed between the execution time and the batch reaction is loaded with the reagent ', 2 : It is to use the 96-hole plate of the sealed film, and the sealing film on it can be used. Test = In the operation experiment, the opening of the hole plate is fast to operate the door, and the second is to use the ultraviolet light sterilization function. Can be cleaned and sterilized to avoid contamination under the batch - 6 M322543 times of the object to be tested. [New content] The purpose of this creation is to provide a kind of automatic magnetic bead operation Ping Am brain with the latest ^ ^, face The humanization and automatic control stability, and increase = automatic magnetic bead operation; the second is clear and easy to operate the platform to play the easy magnetic bead operating platform for medical inspection units and research and development units. Wang automatic station, another purpose Providing a full self The magnetic bead is operated on the flat top; the heating strip, the thermal conductivity block with good thermal conductivity and the flexibility of any previous experiment: the applicability of the user of the ίLu in the operation platform Γ ^ The full-automatic magnetic bead magnet bar The fully automatic magnetic bead operating platform comprises: a computer, wherein the automatic magnetic bead operating platform is in-sliding in front of the front guide =: guide block and ultraviolet light, and the hole plate is used for the above-mentioned automatic magnetic Front panel's front hole, the hole plate is - a hole plate with 96 holes, the front: good, the position, each slot has eight holes. Two of the above heat conduction: the corresponding configuration of the hole plate The heating strip and the tray are provided. The heating strip and the heat conducting block are used to heat the hole 7 M322543 and the heat conducting block is heated to insert the hole plate into the lion sleeve. Further, the magnet bar can adsorb the magnetic beads located in the orifice plate, so that the magnetic beads are attached to the outer wall of the fresh rod sleeve. Preferably, the magnet rod is a permanent magnet. Preferably, the foregoing UV lamps are used for cleaning and sterilization. Preferably 'before The fully automatic magnetic bead operating platform further includes temperature control to provide power control and temperature detection required for heating. The advantages of this creation: 1. Stability of the engineering computer 1 memory =:= gives users more freedom The editing space is edited out = = == more equipped with engineering computer: Windows 昼m will be inserted, the face is better and more humanized, easy to understand and direct touch operation, allowing users to more between the guides, In order to facilitate the placement and removal of the orifice plate, the surface is slipped and during the operation, the hole is held to maintain the fixed hole plate after positioning. 4. Heating by the heating strip and the heat conducting block: == J : The platform can be widely used in various biochemical or two-in-one, and the heating strip and the heat-conducting block are easy to assemble and disassemble. 5.==Light M322543 lamp is used for cleaning and sterilizing the operation closed space in the magnetic bead operating platform , thereby avoiding cross-contamination between batches and batches. [Embodiment] While the present invention will be fully described with reference to the drawings containing the preferred embodiment of the present invention, it should be understood that those skilled in the art can modify the creation described herein. At the same time, I was awarded the work of this creation. Therefore, it is to be understood that the following description is intended to be a broad disclosure of those skilled in the art and is not intended to limit the invention. And ^ refer to the first figure and the second figure, respectively, showing a perspective view and a partial schematic view of the automatic magnetic bead operating platform 1 of the present creation. The automatic magnetic bead operation of the creation includes a computer 2, a magnet bar 3, a mixing rod set 4, a bead 5, a hole plate 6 and a guide groove 7. The computer 2 has a touch panel for controlling The fully automatic magnetic bead operation platform operates. Referring to the first figure and the second figure' and referring to the third figure, the creation of the fully automatic magnetic bead operating platform 1 (4) is the same as that of the bottom of the magnetic bead operating platform 1曰:13, and further comprising a heating strip 8, a heat conducting block 9 and an ultraviolet lamp, wherein the second magnet is included to accommodate the magnet rod 3, the rod #6, the orifice plate 6, the guide groove 7, the heating strip 8, and the guide The external light 10, and the magnet rod 3, the stirring rod sleeve 4, the hole-like = heat-conducting block 9, the heating strip § is sequentially slid from top to bottom in the horizontal direction = 2 6 4: The sleeve can be disposed in the right side of the tray 6, and the ultraviolet lamp 10 can be disposed in the hole wall, the right side wall, the rear side wall or the upper side wall. Left side of the second 3 M322543 • Please continue to refer to the first and fourth figures to show the touch panel u. This is a fully automatic magnetic bead operating platform! Except that the power switch and temperature setting are directly switched by the modular temperature controller 12, the remaining functions are controlled by the touch panel u. When the power is turned on, 'the computer screen appears to be turned on, touch the touch panel u to enter the ^ surface', showing the main function page 'current design: A. program editing, B · program execution, C · temperature control and D·UV light. A. Program editing: The current editing functions include "slot selection", "mixing time", "magnetization time", "heating time", "use of magnetic bar", mixing speed", "solution Parameters such as volume and pause are added or subtracted as needed. Gentleman program execution: "The currently executed action" will appear, and the display will be executed π', in addition to the "pause function" * "stop function. C. Temperature control, can control four sets of temperature controllers individually, There is a control switch for the fan and the buzzer. D. Ultraviolet light can control the switch of the ultraviolet light 10. The user only touches the remote control panel U and follows the window function option to get started, ' In addition to the simple up and down key selection, the English alphabet window is used for reference. 'The user does not need to go through the traditional teaching to practice.' You can familiarize yourself with the operation of the entire automated process. u There are 12 holes in the 6 holes. In the slot (as shown in the third figure, the left and right sides of the hole plate 6 are slot one, slot two, slot three...to slot ten:)' and each slot has eight holes. a trough, a set of eight magnets; 3 with a set of eight stirring rod sleeves 4 can be inserted into any of the slots in the orifice plate 6 H-view of each of the perforated discs 6 Cooperating with two M322543 groups, a total of sixteen magnet rods 3 and sixteen of the stirring rod sleeves 4, so that they can be simultaneously on one orifice plate 6 Sixteen specimens are operated, and one specimen has six slots for use. The automatic magnetic bead operating platform of this creation can be configured with two orifices 6, so the maximum inspection can be processed at the same time. The following preferred embodiments are intended to describe the operation of the present invention in detail, and are not intended to limit the scope of the present invention. Embodiment 1·· • The six slots of the orifice plate 6 are used for purification of nucleic acids. For example, the configuration is as follows: slot one, the product of the lysate; the tank two, the first cleaning liquid; the tank three, the second cleaning liquid; the tank four, the third cleaning liquid; the tank five , magnetic beads and preservation solution; slot six, the extraction liquid. First, the stirring rod sleeve 4 is first returned to the origin for the origin correction. First step: moving the stirring rod sleeve 4 to the slot one, and The stir bar sleeve 4 moves up and down rapidly to cause a flow of liquid in the tank one, and the cells are sufficiently lysed in the lysate to release nucleic acids or proteins. • Second step·· Move the stir bar set 4 to the slot five, and move up and down quickly to disperse the slot five The magnetic beads 5 are then moved downward to be inserted into the mixing rod sleeve 4, and the magnetic beads 5 are attached to the outer wall of the front end of the stirring rod sleeve 4 by magnetic attraction. The third step: simultaneously Moving the magnet rod 3 and the stirring rod sleeve 4 to the slot position 2, and then raising the magnet rod 3, at this time, the outer wall of the front end of the 5-injecting rod sleeve 4 is detached, and the stirring rod sleeve 4 is in the first type g The washing liquid moves up and down rapidly, the magnetic beads 5 are thoroughly mixed with the first cleaning liquid, so that the surface of the magnetic beads 5 reaches the cleaning effect, and then the magnet rod 3 11 M322543 4 is moved down again to make the magnetic beads 5 is again adsorbed on the outer wall of the stirring end. The fourth step: simultaneously moving the magnet rod 3 and the stirring rod sleeve 4 to the tank position, and then raising the magnet rod 3, at this time, the magnetic beads 5 from the The outer wall of the front end of the stirring rod sleeve 4 is detached, the stirring rod sleeve 4 is rapidly moved up and down in the lysing liquid, and the magnetic beads 5 are thoroughly mixed with the cracking test, so that the magnetic beads 5 form a complex with the nucleic acid or the protein, and are adsorbed in the - Up, the iron rod 3 moves down again to make the adsorption and nucleic acid or protein form. The fifth step of the bead 5 being adsorbed again on the outer wall of the front end of the stick sleeve 4: simultaneously moving the magnet rod 3 and the stirrer sleeve, and then raising the magnet rod 3, at which time the magnetic bead is stirred. The outer wall of the front end of the rod sleeve 4 is detached, and the (four) rod sleeve 4 is moved up and down in the ==, and the magnetic beads 5 are completely cleaned, so that the magnetic beads 5 are optimal in the first cleaning liquid. If the non-adsorbed contaminant is removed, then the magnet bar 3 is again moved, and the magnetic bead 5 is again adsorbed to the front end of the stirring rod cover 4. The sixth step: simultaneously moving the magnet rod 3 = °; in the third position, the magnet rod 3 is raised, at this time:,; n; the outer wall of the stirring rod sleeve 4 is detached, the stirring rod sleeve 4 ^ in the washing liquid Moving rapidly, the magnetic bead 5 and the second cleaning entangled the magnetic bead 5 to achieve optimal cleaning in the second cleaning solution, removing non-adsorbed contaminants, and then the magnet bar 3 The magnetic bead 5 is again adsorbed to the front end of the stirring rod sleeve 4; the seventh step is moved: (4) moving the magnet rod 3 and the mixing tank position 4' to raise the magnet rod 3, at this time, The magnetic bead 12 M322543 is detached from the outer wall of the front end of the stirring rod sleeve 4, and the stirring rod sleeve 4 is moved up and down rapidly in the third kind of washing liquid, and the magnetic beads 5 are mixed with the third cleaning liquid to make the magnetic beads 5 in the third cleaning solution to achieve the best cleaning results, remove the non-adsorbed contaminants, and then the magnet bar 3 moves down again, so that the magnetic beads 5 are again adsorbed on the front end of the stirring rod cover 4 On the wall. The eighth step: simultaneously moving the magnet bar 3 and the lion cover 4 to the slot 6, and then raising the magnet bar 3, at this time, the magnetic bead 5 (4) is the outer wall wire of the front end of the mixing rod sleeve 4, (4) The rod sleeve 4 moves rapidly up and down in the extracting liquid °, and the magnetic beads 5 are thoroughly mixed with the extracting liquid, so that the magnetic beads 5+ reach the outermost flushing effect in the flushing liquid towel, and the reduction is proposed. The magnet bar 3 is moved downward again, so that the magnetic bead 5 is again adsorbed on the outer wall of the end, and the cleaned product is the purified nucleic acid. The ninth step: simultaneously moving the magnet bar 3 and the second=: the magnet bar 3 is raised again. At this time, the magnetic bead 5 is detached from the outer wall of the WW end of the stirring rod cover 4, and the wand cover 4 is preserved. The liquid moves gently. The magnetic beads 5 are thoroughly mixed with the preservation solution, so that the magnetic beads release the pure face towel, and then the transfer sleeve 4 moves upward, and returns to the origin, and the program is terminated. Read the fourth picture 'In order to better meet the experimental parameters, the design of the automatic magnetic bead operation platform and the 62 presets are provided with a plurality of strips of heating strips for the second time. 'And between the orifice plate 6 and the heating strip 8 = there is a V thermal block 9 ′ for more uniform and rapid heating effect, here: in the embodiment, the heating strip 8 and the heat conducting block correspond to the hole The slot 6 of the disk 6 and the slot 12 are twelve. The re-processing of the material 13 M322543's conversion ί creation of the whole self-bead operation platform batches and batches, and the use of the ultraviolet light 10 to achieve rapid sterilization of the door Xi > interference of the dye; the user can freely control the time of sterilization of the UV button 10 by controlling the ultraviolet light. In addition, there are two kinds of protection measures: 1. When the door is opened, the i will be powered off for 1 line. After the standby door is closed, the UV lamp is 1〇. The second light is lit and used to avoid the ultraviolet light outside the door when the door is opened, and the transparent plastic door on the cymbal is used to absorb the ultraviolet light. The situation, and there is no need to bear the danger of your field to shoot. 2. If the UV lamp 10 is not turned off, the user cannot execute an automated operating program. Example +: The use of the automatic magnetic bead operating platform 1 of this creation, with 100 μΐ of king blood)=, the whole operation procedure is as follows: After the whole blood is centrifuged at high speed, the upper layer is transparent and colorless. The blood water is used and the orifice plate 6 of the dispensed reagent is used, the sealing film is torn open, and the plasma is placed in the slot. The power of the fully automatic magnetic bead operating platform 1 is turned on, and the power-on surface appears to touch the touch panel. 11 Enter the main page, enter the temperature f system, set the heating bar 8 to make the green state, and sigh the temperature controller's μ degree to 65 C, then enter the program control, select the program, and execute the process, then you can enter Execute the program page, touch the 'return key' of the touch panel 11 (4) the rod sleeve 4 automatically returns to the origin; the β海孔疏6 that has been inserted into the specimen is placed under the orifice plate 6 The heat conducting block 9 is pushed into the guiding groove 7 horizontally together, and pushed to the bottom, and the execution key is touched, so that the fully automatic magnetic bead operating platform 1 automatically starts to operate. First, the M322543 in the slot is sucked: the diacid' then uses the magnet bar 3 and the magnetic bead 5 in the stirring slot 5, and moves to the slot 1 = and the joint is in the slot according to the order Second, the trough-, trough: - and trough four for cleaning, the way of doing the same as the above: plus 』: Ϊ step; after the cleaning is completed, through the thermal block 9, 绅; The groove 6 of the orifice plate 6 is twisted, == is released, and then the magnetic bead 5 is moved back to the groove position; and, "the money mix rod set 4 is also returned to the origin, and the program can be terminated. The creation provides a fully automatic magnetic bead operation platform 1, which can be used for heating, sterilization and computer operation, providing users with an integrated magnetic bead operation flat. The lack of the traditional magnetic bead operating platform. After the description of the preferred embodiment of the creation, familiar with the understanding of the technology, without departing from the scope of the following patent application and the various changes and changes, and the creation It is not limited to the embodiment of the embodiment set forth in the specification. 15 M322543 [Simple diagram The first picture shows the perspective view of the automatic magnetic bead operating platform of this creation. The second picture shows the partial schematic diagram of the automatic magnetic bead operating platform of this creation. The third picture shows the installation of the automatic magnetic bead operating platform of this creation. The structure in the space is not intended. The fourth figure shows the schematic diagram of the touch panel. [Main component symbol description] 1... Fully automatic magnetic bead operating platform 2 - Computer 3 - Magnet bar 4... Stirring bar set 5... Magnetic beads 6 — Hole plate 7 — Guide groove 8... Heating strip 9 — Thermal block 10 — UV lamp 11 — Touch panel 12 — Temperature controller 13 — — Accommodation space 16