TWM258282U - Biochemical electro-optic detection system with dual optical path and of minute absorption type - Google Patents

Description

M258282 3. Creation instructions α) The original creation belongs to the new type. The dual optical path has a very small amount of micro-absorption, and it uses the advantages of light-emitting diodes. : Previous technology] The biochemical measurement method, that is, the use of the sense is the reagent method, that is, photometric technology into the technical field related to a type of biochemical photoelectric detection system photoelectric detection system ', low price, double light can eliminate the use of filter-receiving biochemical The simple body of the system is a light source system, especially a method that has both the sensitivity of the process required for the specimen and the first method due to the film and no heat problems. Yes, the light absorption method, these methods, and the wavelength region volume measurement are accurate. The second method is to detect and measure the difference in biochemical properties. The measurement method has a low measurement and multi-parameter degree, and a method for quantitative analysis of the analyzer; and the reagents for reagent detection, and the quantitative measurement method, the basic method of the fluorescence instrument, and the outside line of the spectrum can be divided into chemical conversion reagents or quantities; Its quantity is easy to measure at the same time and has good reproducibility. The disadvantage of the universal application is that there are two main types of physical spectrometers: the main material light method with environmental methods. Change to the electricity test strip test, the device is small, and the performance is not good. It is suitable for a large number of devices, and it becomes a complicated pollution of the equipment. It is necessary to test the hand composition and turbidimetric method, and commonly known as photochemical analyzer, near-infrared, etc. are sensor detection for measurement. Another type of biochemical components, and further the use of type, fast speed, prone to reagent contamination, etc. of the sample ’s pre-physiology, is not suitable for comparison, the first-line spectrum inspection of the amount of square absorption spectrometer phase instrument, so the scientific research is the most intensive Complex in the body. It is similar to using the material method, including the ratio method. Species, as applicable

Page 6 M258282 9. Creation instructions (2) Spectrometer. Ultraviolet and visible spectrometers are often used for color measurement and biochemical inspection, while near-infrared spectrometers can be used for process monitoring in the food and pharmaceutical industries. Infrared spectrometers are often used for gas analysis. The characteristics of spectral analysis include: non-intrusive, non-destructive discrimination, wavelength flexibility, high sensitivity, and analysis speed. It has many advantages and can be applied in a wide range, such as: gas, medical laboratories, automotive sheet metal, printing coatings, food Processing, luminescence is abbreviated as LED, etc.), but the current popularity in the industry may be due to the high unit price of the spectrometer, its large size, and the complexity of routine calibration procedures. Modern micro-spectrometers can act as eyes. They are small and easy to install on production lines to analyze light sources. For example, LED production lines can use micro-spectrometers to measure the light intensity distribution of LEDs. The food manufacturing industry can improve the quality control standards by using the proportion of each component and the uniformity of mixing. In terms of environmental protection, the spectrometer can act as an inspector in any corner of the site to detect air quality at any time, and to guard against employees' safety. In terms of wastewater discharge, the Department has tested the composition of the microfluidic analysis system and confirmed that it conforms to environmental discharge, which can effectively protect our living environment. The types of spectrometers commonly used in biochemical inspections are quite similar. The spectrometers are bulky and expensive, so they are not mentioned here. The miniature has many advantages not available in large spectrometers, such as weight, water quality, industrial materials, etc., and fast. The photonics and raw diodes do not protect the corners and materials of the operating eyes and the intensity of light is emitted. The micro-spectrum is used to analyze and analyze the chemical spectrum differentiation (in terms of wave color, color, and similarity). , Digital color measurement, combined with the working system, more wastewater treatment standards after finishing, the large optical spectrum analyzer is light and small

Page 7 M258282

, Fast detection f, easy to use, integrated, can be mass-manufactured and low cost, etc., but because of the use of microelectronics technology made of micro-optical detection unit sensitivity is poor, in order to be practical in biochemical detection and Some distance. With reference to the first picture, the basic structure of the conventional micro-spectrometer system is composed of two parts: the optical system and the electronic circuit (not shown in the figure, together with Chen Ming). The optical system part contains the traditional light source 9 of tungsten filament lamp, the light used for collecting light = lens 9 2 three-way beam splitter or fiber coupling element 9 3, micro-diffractive element 9 4, loss 2. The emission spectrum of the traditional light source 91 of this tungsten filament lamp needs to have a wide absorption r-band. Optical lens 92 must be in accordance with the light guide system used, the numerical aperture of the lens is slightly wound = pieces Ι and; = after the fiber is divergent light, so the light ... ability, photosensitivity "second search = analogy; ϊϊ: iii: 9 电Record the spectral intensity and convert, drive circuits, and digital circuits. The architecture includes the front-end circuit to measure the timing and signals required by the components. The main function is to provide functions such as sensing. In addition, it is based on i A 'A / D conversion and image transmission design. The overall structure is quite complex to avoid the use of W or high-frequency circuits. Traditional large or miniature rectangular quartz containers of the inverse production specifications are used when the biochemical standard is assumed to be popular in an atypical severe respiratory syndrome (abbreviated as sars).

M258282 IV. Creation instructions (4) In the region, the local medical inspection unit must collect the patient's body fluids for testing and screening. In case the patient to be tested coughs up a small amount of sputum, it is only enough to make a 0.5cc sputum solution test. Body, because it is less than the minimum volume of 1 cc of the traditional rectangular quartz container, the traditional spectrometer cannot detect it at all. It will miss the golden opportunity to determine whether the virus is infected with the virus, which is not only disadvantageous to the patient, but also adds to the uncertainty of the public. Feeling and psychological panic. However, simply reducing the capacity of the rectangular quartz container from the original 1 c c to 0.5 cc will cause the sensitivity of the instrument to become one-half of the original and may not be detected, which cannot solve the problem. In addition, because different colored materials have different absorption spectra, when a full-spectrum tungsten filament lamp is used as a light source, an additional filter must be added to screen out the absorption spectrum that matches the sample, and tungsten filament is used for a long time. The lamp will have heat problems. Therefore, the disadvantages of the traditional detection system are as follows: [1] The amount required for the specimen is large. [2] The optical system is complex and expensive. [3] The sensitivity of single optical path is low. [4] Tungsten filament lamps require filters and have heat problems. Therefore, it is necessary to develop a new detection system that can detect only a few specimens, has high sensitivity, and is low in cost, which can be afforded by various medical units. [New content] The main purpose of this creation is to provide a dual optical path micro-absorption type biochemical photoelectric detection system, which has a minimum amount of specimens required and high sensitivity of the dual optical path.

Page 9 M258282 4. Creation Instructions (5) The second purpose of this creation is to provide a dual optical path micro-absorption type biochemical photoelectric detection system with simple optical system and low price. Another purpose of this creation is to provide a dual-optical path micro-absorption spectroscopic biochemical photoelectric detection system that uses a light-emitting diode as a light source, which can eliminate the use of filters and avoid heat generation problems.

In order to achieve the foregoing objective, the present invention provides a technical solution as follows: A dual-light-path micro-absorption type biochemical photoelectric detection system includes: a micro-container having a circular receiving recess with a predetermined depth and a predetermined diameter for receiving A specimen, the microcontainer has a first end and a second end, and a chirping reflection sheet is arranged near the first end of the microcontainer. The chirping reflection sheet is composed of a plurality of minute reflective right-angled edges. A mirror is used to reflect the light beam projected on it back along the original path; a collimating lens is provided outside the second end of the micro container; a beam splitter has a beam splitter, an introduction fiber, An outgoing optical fiber and a bidirectional optical fiber; a light source for generating a first light beam with a predetermined wavelength range and an initial intensity; a detector for detecting the final intensity of the received light;

A signal comparison unit is used to compare the difference between the intensity of the sample to be measured and the intensity of the reference sample, to calculate the light absorption of the specimen, whereby the introduction fiber is used to guide the first beam and penetrate the beam splitter. The beam splitter enters the collimating lens through the bidirectional fiber, and then expands and becomes a second light beam close to the predetermined diameter. The second light

Page 10 M258282 IV. Creation instructions (6) The beam passes through the trace container and becomes a third beam, which is reflected back by the krypton reflecting sheet, passes through the trace container and becomes a fourth beam, and then enters the collimating lens. It becomes the fifth beam focused by the beam, returns to the beam splitting prism along the bidirectional fiber, and is reflected and diverted to the lead-out fiber to reach the detector. The signal comparison unit can then calculate the number of specimens in the trace container. absorption rate. The above-mentioned objects and advantages of this creation can be easily understood from the detailed description and accompanying drawings of the selected embodiments below. The following examples and drawings are used to explain this creation in detail: [Embodiment] Please refer to the second to fourth drawings. This creation is a dual optical path micro-absorption spectrum biochemical photoelectric detection system, which mainly includes: The container 10, a prism reflection sheet 20, a collimating lens 30, a beam splitter 40, a light source 50, a detector 60, and a signal comparison unit 70. Regarding the micro-container 10, the micro-container 10 has a circular recessed recess 11 with a predetermined recessed depth Η and a predetermined recessed diameter D for containing a specimen 12, and the micro-container 10 has a first end 10A and One second end 10B. The chirped reflection sheet 20 is located near the first end 10 A of the micro-container 10, and the chirped reflection sheet 20 is composed of a plurality of minute reflective right-angled chimes 21 Reflect the light beam projected on it back along the original path. The collimating lens 30 is provided at the second end 1 B of the micro-container 10 and outside the beam splitter 40. The beam splitter 40 has a beam splitter 4b, an introduction fiber 4 2, an output fiber 4 3, and a Bidirectional fiber 44.

Page 11 M258282. IV. Creation instructions (7) The light source 50 is used to generate a first light beam L 1 with a predetermined wavelength range and an initial intensity; the detector 60 is used to detect the received light The final intensity of light; the signal comparison unit 70 is used to compare the difference between the intensity of the sample to be measured and the intensity of the reference sample, whereby the introduction fiber 4 2 introduces the first beam L 1 and penetrates the beam splitter The 40 beam splitting prism 41 passes through the bidirectional fiber 44 and enters the collimated lens 30. The diffused parallel beam becomes a second beam L 2 close to the predetermined diameter D, and the second beam L 2 passes through the After the micro container 10 becomes a third light beam L 3 (because part of the energy of the light beam is absorbed by the subject 12, the third light beam L3 is weaker than the second light beam L2), it will be reflected back by the chirped reflection sheet 20, After passing through the micro container 10, it becomes a fourth light beam L4 (partial energy of the light beam is absorbed by the subject 12, so the fourth light beam L4 is weaker than the third light beam L3), and then enters the collimating lens 30 and becomes light The concentrated fifth light beam L 5 is returned to the beam splitter 4 1 along the bidirectional fiber 4 4 It turned again reflected to the optical fiber 43 and the derived arrival of the detecting device 60, which in turn may signal comparison unit 70 calculates the trace of the container 12 of the light absorbance of the specimen 10. The path of the beam travel of this creation has been detailed in the previous paragraph, and the basic principle of this creation is to detect the attenuation of the visible light collimated light wave after passing the subject 12 times (that is, every time the subject passes 12 times Attenuation once), and the light absorption of the sample 12 is obtained. The object measured by this system is the coloring substance of the specimen 12 after biochemical reaction. The color depth of the coloring substance solution has a certain relationship with its concentration. When the concentration is large, the color is dark. When the concentration is small, the color is light. The density of the test object 12 can be measured by establishing the color depth and

Page 12 M258282 I IV. Creation instructions (8) The concentration relationship table of biochemical components is used to measure the concentration of biochemical components contained in specimen 12. Immunoreactive antigens or antibodies in biochemical tests are often connected with gold colloids as coloring substances. When the concentration of the colored substance changes, the voltage of the photo-sensing unit also changes. The voltage has a certain relationship with the amount of concentration change. The analog output of the photo-inductance unit can be directly measured by the digital change meter. Voltage. After the above experiment, a graph showing the relationship between the concentration change of the colored substance and the voltage can be obtained, and this graph can be used for subsequent concentration measurement. The specific specifications of some components of this embodiment are listed as follows: Each optical fiber (including lead-in fiber, lead-out fiber and bidirectional fiber) has a diameter of 1.0 mm; the numerical aperture (NA) is 0.44 of the multimode plastic optical fiber; the light source is 5A / W The light-emitting diode, whose spectrum is shown in the fifth figure, the peak wavelength is 656nm; the half-wave width is 18nm; the detector is a Si PIN photo detector (Si PIN photo detector), the sensitivity is about 0.5A / W . The diameter D of the recessed portion of the circular accommodating recessed portion 11 of the micro-container 10 is 5 mm ((K 5cm), and the depth D of the recessed portion is about 0.1 mm (0.01 cm), so the volume of the specimen 12 is only (3 · 14x0. 5Λ2) / 4χ〇 · 01 and 0 · 0 0 2cc, that is, about two thousandths of a cc. The optical system is arranged as shown in the third figure, and the relevant conditions of the test are as follows: Reagent: a coloring substance (a blue water-soluble sodium Rice ball reagent) plus reverse osmosis (R. 0.) filtered water to dilute to an appropriate concentration. Others: a. The test solution is loaded in the original micro container. B. The test results are shown in Figures 6 and 7 .

Page 13 M258282 g, Creative Instructions (9) The sixth picture shows the test results. The concentrations between 1% and 0% and between 1% and 1% are two straight lines with different slopes. The concentrations are lower than One thousandth or more than one tenth of the original reagent is indistinguishable. The results of local magnification and linear analysis with a concentration boundary between 1% and 0.1% are shown in the seventh figure. Therefore, when the light absorption of a certain test object 12 falls within the above-mentioned reliable calibration interval, its relative concentration value can be deduced. In summary, the advantages and effects of this creation can be summarized as follows: [1] The amount of specimen required is very small. The volume of the specimen required for the micro-container of this creation is positively small 'approximately two-thousandths cc' is approximately the traditional Yuan low specimen; s 1 cc is one-fifth of the cc, in other words, a very small number of specimens can be Tests have wider applicability in medical or biochemical tests. [2] The optical system is simple and inexpensive. The system of this creation is simple, and it can be made into special models, such as XX virus micro-detector, XX substance concentration detector, which has excellent commercial promotion. [3] The sensitivity of dual optical path is high. The unique dual optical path design of this creation changes the number of times the light beam penetrates the subject from one time to two times, so the overall sensitivity is improved. [4] The use of light-emitting diodes can save the use of filters and no heat problems. Since the light source used in this creation has a spectral distribution similar to the absorption spectrum of the coloring substance, no additional filter is required, so the use of the filter is eliminated and there is no heating problem. The above is only a detailed description of the feasibility of this creation by means of a preferred embodiment, and any simple modifications and changes made to this embodiment will not depart from the spirit and scope of this creation.

Page 14 M258282

Page 15 M258282 Simple illustration of the drawings [Simplified illustration of the drawings] The first picture is the traditional style, the second picture is the creation, the third picture is the creation, the fourth picture is the creation, the fifth picture is the creation, and the sixth picture is the creation. The seventh diagram is a schematic diagram of the basic structure of the created micro-spectrometer system. The unintended optical system part of the optical system is not intended. The unintended beam path is simplified. The unintended light source is a spectrogram. The test result is a partial enlarged view of the test result. Trace container 10 First end 1 0A Second end 1 0B Circular receiving recess 1 1 Specimen 12 Prism reflector 20 Reflective right-angle 稜鏡 2 1 Collimator lens 30 Beam splitter 40 Beamsplitter prism 41 Leading optical fiber 42 Leading optical fiber 43 Bidirectional Optical fiber 44 Light source 50 Detector 60 Signal comparison unit 70 Optical lens 91 Conventional light source 92 Beamsplitter element 93 Micro-diffraction element 94 Photoreceptor element 95 Recess depth Η Recess diameter D Numerical aperture (NA) First beam L1 Second beam L2 Second Beam L3 Fourth beam L4 Fifth light L5

Page 16

Claims (1)

M258282 _ Case No. 92221947 V. Application scope 1 · A dual-light-path micro-absorption biochemical photoelectric detection system, comprising: a micro-container having a circular receiving recess with a predetermined depth and a predetermined diameter for accommodating an inspection The micro-container has a first end and a second end; a prism reflection sheet is not located near the first end of the rotator, and the reflection sheet is composed of a plurality of small reflective right-angled edges. A mirror is used to reflect the light beam projected on it back along the original path; a collimating lens is provided outside the second end of the micro container; a beam splitter has a beam splitter, an introduction fiber, A lead-out fiber and a bidirectional fiber; 'a light source for generating a first light beam with a predetermined wavelength range and an initial degree; σ a detector for detecting the most powerful product of the received light The unit is used to compare the strength of the sample to be measured. “Reference sample and body.” The Diaobayou fiber system introduced the first beam and penetrated the beam splitter of the beam splitter, passed through the two-way straight lens, and then expanded and changed. Into a near-predetermined diameter = beam, the second beam becomes a _ "a Will be reflected back by the chirped reflection sheet, and then pass through -beam-fourth beam, then enter the collimating lens core c to change the beam, return to the beam splitter along the bidirectional fiber to the exit fiber and reach the exit fiber Detector, skin reflection M258282 Rev. _ Case No. 92221947 V. Scope of Patent Application The absorbance of the sample in the trace container is calculated by the unit. 2 · The dual optical path micro-absorption type biochemical photoelectric detection system according to item 1 of the scope of patent application, wherein the micro-container is a rectangular transparent plastic flat plate with a circular flat groove-shaped circular receiving engraved on it 1 至 0, 5 毫米 之间。 The diameter of the recess of the circular receiving recess is 4 to 6 millimeters (mm), and the depth of the recess is between 0.1 to 0.5 millimeters. Page 18