TWM243482U - A biochemical reaction apparatus - Google Patents

Description

M243482 Case No. 92211992 Amendment V. Creation Instructions (1) [The original creation of the new type is especially to provide anti- [previous technology] "Nucleic acid heterogeneous step through design (target) technology has been widely used due to performance, nucleic acid clinical detection ... Generally speaking, the most critical ones include Dot b 1 ot 4-1 Θ hours. The operation process is too high and the heat capacity is too high. Because the current market is more than 20 kg, adding a hybridization reaction test flask, and then using it to generate a disturbance. However, The best mixing effect of rotating by line, so moving (about 1Hz) _ 丨 叱 川 丨. 丨 幻 · w ,, 【. 机

[Technical Field] Regarding a biochemical reaction box, which is applied to biochemical reactions, the reaction of soil and soil according to the temperature and the amount of vibration required for the reaction is actually "reaction" means that the nucleic acids become complementary due to complementation. The nucleic acid probe (pr 〇be) can be used for detection after hybridization reaction with the nucleic acid target. The gold% correlation is used for the sub-biological laboratory, for example: gene selection sequence = nucleic acid mutation analysis, disease detection Divided and micro-amplified, nucleic acid hybridization is a procedure for molecular biological detection. However, in the process of nucleic acid hybridization, the reaction time required for,, Southern blot, and microarray, etc., causes a lengthy reaction process. The reason is not only complicated, but also because the temperature of the hybridization reaction box is too large = p, which causes the heating process to be too slow. The hybridization reaction chambers sold on the counter are bulky and heavy, and they have been heated for too long. In addition, the mixing effect of the reagent in the hybridization reaction box is to first fix the test tube through a clamp ^ by a rotator in a rotating or rolling manner to achieve the purpose of mixing and accelerating the reaction. Since the device is driven by a motor to drive the reaction sample into 40% of the disturbance or the vibration frequency is generally low frequency, the heat reaches the molecules in the solution generated by the high frequency disturbance. If the reaction sample reaches the high frequency disturbance, M243482 amended

-SS_J22n992 V. Creation Instructions (2) It is necessary to increase the rotation speed of the rotating device to make it rotate at high speed. However, when it is set to rotate at a high speed, the reaction sample will be subjected to centrifugal force, 2 the effect of separation, and cannot help to react to the sample. Disturbance and mixing. = The rotating device composed of Lai has a lot of zero rainbow parts (crank, cam ... finding), so it will increase the volume of the whole hybridization reaction box. And some heterogeneous reaction boxes use multiple measures. Compared with: the mixing effect, relatively, it will increase its rotation in one direction. Now, it may be necessary to consider the rotation devices of different axial directions at the same time. ), Due to the turning of the car, the production cost of the entire hybridization reaction box was raised, ^ [new content] The difficulty of making the long clothes. Therefore, if we can develop a small and 丄 can only reduce the waste of experimental space, i: = into the hybridization reaction box, not cost, making it a real desktop type; In view of the problems of the above-mentioned conventional technologies, a biochemical reaction box is applied to the nucleic acid beach. The purpose is to provide a required reaction overflow and reaction time. 1 = reaction t in order to provide a reaction sample point lies in the vibration / reaction environment in this biochemical reaction box, and its special reaction sample placement plate generates water and money. It uses magnetic force to make the effect necessary to provide the biochemical reaction. High; = the amount of vibration in the direction, the effect of vibration is generated by the magnetic rotation device A = rate vibration, the volume occupied by the conventional reaction box does not need to be used, reducing its t 'can greatly reduce the entire biochemical simplification, Conducive to mass production. /, Σ 卩 's number of parts to make it structure

M243482 V. Creation instructions (3) This biochemical reaction box is composed of an inner box and an outer box. The inner box (ie, the insulation heating chamber) mainly includes: a bearing base, a placing plate, an electromagnetic suction, a heating plate and Spoiler fan. The s-disc is placed on the base of the carrier in a “movable” relationship to carry the sample for biological reaction, and a pair of permanent magnets are set on one side of the disposition. The electromagnetic iron corresponds to the position of the permanent magnet, and the direction of its magnetic field is changed by the cross current applied to ΠΓΐ, so that the permanent magnet generates an inexhaustible force, and then the sample to be reacted on the plate is placed in a horizontal direction. The effect of vibration. i 生 ΐ Ϊ: ΐ Π: The mechanism that provides the heating function is to use two pieces to heat Vi :: and the upper and lower sides of the placing plate to heat the placing plate 2: ifi samples. The turbulence fan is set on the heating plate: the hot plate can heat the biological reaction more evenly. 1: Now the biochemical reaction box of Luzhou City's Institute of Biotechnology has the following advantages: The size is greatly reduced by two. Make it Λ straight xx 3 2 cm), which can effectively reduce the waste of space ^ and become a real desktop experiment equipment. Buy Born. What's more, the equipment used in the past is larger than that? n k ,, should be only 6.5kg, which can achieve light weight and high quality. At present, it is controlled by one J, without the need of T /// two-by: V; the exchange of electromagnetic attracting iron; the dynamic system is simple in construction and can reach the cattle, not only the variable vibration efficiency. Dry ’and can reach strong

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92211QQ9 V. Creative Instructions (4) From the above, we know that this biochemical reaction box. Reduce the cost required for production, and can achieve a good volume reduction of good V. Therefore, it becomes a feasible "good; 2 temperature and vibration are specially made for the purpose of this creation, chess 1 dry market / shallow market forces Products. I understand that I have a H-M structure k4 inch sign and its functions have further

The detailed description of J liver-level heart mouth map is as follows: J [Embodiment] The view of Li Lin's ^ 1 picture "What is not, this is the biochemical reaction box of this creation. It contains the following main parts and control panels 2〇 , Door handle 30, front door panel 40 and handle 50. The load bearing system is used to house the insulation heating chamber and its internal components, including: components, actuated thunderbolts, and ice; used to produce vibration and heating effects. The external factors required in biological reactions are external factors such as vibration, temperature, etc. The panel 20 includes a power switch 21 and a vibration control rotary controller 23. The power switch 21 is used to control the power supply for starting the biochemical inverse reaction Γ. The vibration control knob 22 is used to control the sample reaction time, and the item temperature is used to control the temperature inside the biochemical reaction box. Temperature of the insulated heating chamber inside the case body 10 during the sample reaction.门 The door handle 30 is set on the front door plate 40, which is used to pull out and push in the drawer body 10 from the box body 10 in the form of a drawer to accommodate the reaction mechanism. The sample to be reacted or the reaction is completed. The λ of the handle 50 is convenient for users to carry this biochemical reaction box to different places to carry out the nucleic acid hybridization reaction.

M243482

Case No. 922Π992 V. Creation Instructions (5) 1 Figure 20, Three-dimensional of the mechanism in which /? Causes the response sample to vibrate horizontally. The u 3 has the following main parts: the bearing base 60, the placing plate, the water long magnet 71, and the electromagnetic iron 80. The p-disc 7G is a load-bearing tray, which is used to transfer the f-disc 7 °. It is set on the load-bearing base in a movable relationship. Xu Xi 7 can be moved to the load-bearing base 6 to make samples. Can produce water 71. A pair of permanent magnets w is provided on one side of seventy seventy. = The outline corresponds to the mechanism of the permanent magnet fruit on the placing plate 70. The horizontal direction of the vibration effect generated by the reaction sample carried will depend on the force :: J f: 80: After the AC power is applied, the magnetic field generated by it will be generated. Periodic changes :: ;; Rate: ;;; Yu Gong: Suction: 80 iron 7 1 and the bamboo people are attracted and repelled by the long-term permanent magnetism of the water sigh placed in their squares *, and Due to the market ?. -Weeks; = above, thus indirectly providing the effect of placing vibrations. Therefore, the placing plate 70 produces a horizontal direction. So, the frequency of the alternating current usually used is 50-60 Ηζ, and the vibration frequency of the conventional hybrid 丄 丄 reaction box can reach a minimum of 50 Η ζ, Compared to the #remove reaction box, the vibration frequency (1 Ηζ) is much higher. Type, change the electricity ^ want to change the vibration frequency of the placing plate 70, 'can be changed through the frequency conversion side vibration frequency ^ absorption of magnetic pole conversion frequency' to make the system higher iron = more vibration intensity of the placing plate 70, can be permanent magnetic ~; --1 ^ Yes, you can block the user's resistance.

Page M243482: One '^ · ~ ^ 211992_ 色 _ Month and Day Amendment V. Creation Instructions (6) ----% P value, adjust the size of the permanent magnet 7 1 supply voltage, you can change the disc 70 The intensity of vibration. A connection relationship between a carrying base 60 and the placing plate 70 is as shown in FIG. 2. A first screw 61 is provided on the edge of the carrying base 60, and the & edge of the placing plate 70 corresponds to The first screw 61 is provided with a second screw 72, and the groove on the supporting base 60 for receiving the second screw 72 has a large space, so that the second screw 72 can be placed in the space. mobile.

The elastic yellow 90 is connected between the first screw 6 丨 and the second screw 72. When the placing plate 70 is vibrated by the attraction or repulsive force of the electromagnetic attracting iron 80, the elastic yellow 90 will generate a restoring force. The second screw 72 is driven to move, so that the placing plate 70 can generate a horizontal vibration corresponding to the bearing base 60. Between the contact surface of the placing plate 70 and the bearing base 60, a plurality of balls 73 are provided to reduce the resistance and collision caused by surface friction when the placing plate 70 moves relative to the bearing base 60, so as to strengthen the placing plate. 70% vibration effect, and reduce the noise generated during vibration. The load-bearing base 60 is fixed on the ball slide rail 62 and is used for a pull-type switch. Please refer to "Figure 3" to "Figure 5" for a perspective view of the heating plate and the turbulent fan in the biochemical reaction box to provide heating function. The upper heating plate 101 and the lower heating plate 102 are sandwiched between a carrying base and a placing plate.

The upper and lower sides of the 70 are fixed to the heating chamber cover 1 2 1 and the heating chamber without the cover 122. The upper and lower sides of the placing plate 70 are heated, and then they are operated with a spoiler fan 110 installed on one side. It can effectively and uniformly heat the sample to be reacted on the placing tray 70. The upper heating plate 1 Q 1 and the lower heating plate 102 may be resistance heating metal plates. The upper heating plate 1 0 1 and the lower heating plate 1 0 2 are located in the insulated heating chamber 1 2 〇

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Case No. 92211992 V. Creation Instructions (7) The insulation heating chamber 12 is provided with a layer of thermal insulation covering the outer system to the above ^ 21 哉 ^ heating chamber 120, which makes the heating chamber: Two :: Force: The performance of space and the external environment can also avoid not: even sentence performance has better power consumption. The thermal failure of I reduces the η η required for the use of the instrument, while Bu Jiuga plus hot plate-1 〇1, the lower heating plate 10 2 is set with a temperature-sensing single-μ inner neighbor: a soap 13 0 series It is a temperature sensor to measure the biochemical residual temperature: the temperature inside the phase, and the sensor = the biochemical reaction hot plate ιοί and the emperor of the lower heating plate 102 are fed back to the control increase rate, and the biochemical reaction box敫 :: Individual heating power. The brewing degree of 1 is adjusted to the temperature set by the user. Welding Shiming * Kao 6 Figure "", is a temperature monitoring measurement kit for measuring the actual temperature in the biochemical reaction box. ; 3 = The kit 14〇_ is made up of several thermocouple wires, and it is ς. Off = placed on the front door panel 40, and the digital temperature is matched by thermocouple wire, excluding 1 4 1 for temperature measurement. ^ Connected through the thermocouple wire insertion holes 41 on the front door plate 41 to different reaction sample positions on the setting plate 70. The temperature of multiple measurement points and the temperature changes at each point in the two-K phase built-in plate 70 are used as It is used for temperature and quality test at the time of production.

Page 11 Please refer to "Figure 7" for another embodiment of the connection method between the bearing base and the placement ㈣. The load-bearing base 60 is a frame provided on the periphery of the setting plate 70, and a groove is respectively provided on the corresponding surface of the load-bearing base 60 and the setting plate 70, so as to jointly form a service channel 150. There is a sliding ball 160 in the track 15 ′. The magnetic field on the disc 70 is too large. The magnetic error is 71. M243482. Case No. 922] January. Revised 5. Creation instructions (8). When a magnetic field with a variable change is applied, it can cause horizontal vibration with respect to the bearing base 60. As shown in "Annex 1" of Qingmai test, it is the result obtained by measuring the temperature between the upper heating plate 1 〇1 and the lower heating plate 1 0 2 using a temperature sensing unit 丨 30. The horizontal axis is time (shift) and the vertical The axis is the temperature (C). As can be seen from the figure, the biochemical reaction chamber can be quickly heated from room temperature to 50 ± 0.5 within 5 minutes. (: And under long-term monitoring, 'its temperature stability can reach ± 0. .., because of & when the heating effect is excellent. Two: pieces 2 "shown'" several thermocouple wires are set on Place the U ί hole plate with the same number, and set the temperature to 50 ° C, and add it. After waiting for about ten minutes to reach a steady state, the temperature in the 盘 ς plate is The horizontal axis is a hole with different numbers. 2 Different numbers of temperature (.0, as can be seen from the figure: each different number | while the vertical axis is around ㈣, and its temperature is up to ± 0.5; The temperature about the above is only the best of this creation and is not used to limit the scope of implementation of this creation; that is, all equal changes and modifications made according to the original creation, and all around, are the creation patents: : Please be covered by the patent fan blade target enclosure. «

M243482 Case No. 92211992 __Feng Schematic Explanation " ^ ------— The first figure is a three-dimensional view of the appearance of the biochemical reaction box; the standing of the mechanism used to generate the vibration effect in the reaction chamber ~ brother Figure 3 is a t-lid diagram in the biochemical reaction box. ·, The figure is used to generate heating effect in the biochemical reaction box ::::: 之 立: 图; = ί ί ΐ The stand of the institution Ξ: The figure is a perspective view of the temperature monitoring measurement kit; 'Γ part drawing ί schematic diagram of another connection method between the bearing base f and the placing plate; The temperature measurement unit is used to measure the upper heating plate and the lower heating The relationship diagram; and ^ is to set several thermocouple wires in different perforated disks on the placing plate, and set the degree to 50 t. After the heating is completed and the steady state is reached, measure the different numbers of the perforated disks. Diagram of temperature and its location. [Illustration of Symbols] 10 20 30 40 41 50 60 61 62 70 71 72 Box Control panel Door handle Front door panel Thermocouple wire placement hole Handle _ Loading base First screw Ball slide rail Placement disc Permanent magnet Second screw

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Brief description of the drawing 73 Ball 80 Electromagnetic attractor 90 Impeachment 101 Upper heating plate 102 Lower heating plate 110 Spoiler fan 120 Insulation heating chamber 121 Heating chamber upper cover 122 Heating chamber lower cover 130 Temperature sensing unit 140 Temperature monitoring measurement kit 141 Digital Thermometer 150, 160, ball

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Claims (1)

M243482 Case No. 92211992 A. Application Patent Scope 1. A biochemical reaction phase is used to provide a reaction environment required for a biochemical sample, which includes: ~ < A cabinet having at least one electromagnetic attracting iron in the cabinet ; And, a placing plate is set in the box in a movable relationship for placing biochemical samples, and the placing plate has more than one permanent magnet corresponding to the electromagnetic attracting iron, which generates a magnetic field against the current of the electromagnetic attracting iron. The permanent magnet generates mutual repulsion and mutual attraction ', so that the placing disk reciprocates in the box body, and generates a vibration amount. Ml 2 · According to the biochemical reaction box described in item 1 of the scope of patent application, by adjusting the frequency of the alternating current applied to the electromagnetic attracting iron, changing the magnetic pole changing frequency of the electromagnetic attracting iron to change the vibration frequency of the placing disk. 3. The biochemical reaction box as described in item 1 of the scope of patent application, wherein the permanent magnet is coupled to a variable resistor, and by adjusting the resistance value of the variable resistor, the magnitude of the voltage supplied by the permanent magnet is adjusted to Change the intensity of vibration. μ direct enemy a 4 · The biochemical reaction box according to item 1 of the patent application scope further includes a supporting base, which is arranged in the box to carry the placing plate. 5. The biochemical reaction box as described in item 4 of the scope of patent application, wherein more than one ball is not placed between the contact surface of the bearing base and the placing plate to reduce the placing plate relative to the bearing base. The frictional resistance generated when moving. No 6 · The biochemical reaction box as described in item 4 of the scope of the patent application, wherein the bearing base is provided with a track corresponding to the placing plate and is accommodated in the track. Page 15 M243482 Case No. 9221〗 2 6. One or more balls with patent application scope, so that the f disc can move relative to the bearing base. 7. The biochemical reaction box according to item 4 of the scope of patent application, wherein a first screw is provided on the supporting base, and a second screw is provided on the placing plate corresponding to the first screw, and a spring system is provided. It is connected between the first screw and the second screw, and the placing disk can move relative to the bearing base by the elastic yellow telescopic. 8 · The biochemical reaction box as described in item 4 of the scope of patent application, wherein the bearing base is connected to the | self by a ball slide rail, so that the bearing base and the placing tray are in the form of drawers. Pull out and push in the box. 9 · The biochemical reaction box described in item 1 of the scope of patent application further includes two heating plates' sandwiched on the upper and lower sides of the placing plate to heat the sample to be bioreacted. 10. The biochemical reaction box as described in item 9 of the scope of the application for a patent includes a pack of 1-7;] L feather 'causes the heating plate to uniformly heat the sample to be subjected to a biological reaction. 1 1 · —The biochemical reaction box described in item 9 of the scope of patent application further includes a space heated to ′ to surround the space to be heated to avoid the loss of heat energy. 12 女 u The biochemical reaction box described in the patent application No. 丨 丨 ^ P-like material, which is wrapped around the periphery of the heating chamber to avoid the dissipation of thermal energy if ^ The biochemical reaction described in item 1 of the patent application scope The box also includes the first, right, W-degree sensing cry, and π 52 3 / ware temperature production. If it is placed in the box, it can sense the biochemical reaction box described in item 1 of the patent application scope of Shishenyu, including a biochemical reaction box. Page 16 M243482 _ Case No. 92211992 _ Month and Day __ VI. Patent application scope Detachable temperature measurement kit can be used to measure the temperature of multiple measurement points and the temperature change of each point in the placing plate in the box at the same time .