TWI878377B - 陽離子交換層析期間之高鹽洗滌以去除產品相關的雜質 - Google Patents

陽離子交換層析期間之高鹽洗滌以去除產品相關的雜質 Download PDF

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Publication number
TWI878377B
TWI878377B TW109138883A TW109138883A TWI878377B TW I878377 B TWI878377 B TW I878377B TW 109138883 A TW109138883 A TW 109138883A TW 109138883 A TW109138883 A TW 109138883A TW I878377 B TWI878377 B TW I878377B
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TW
Taiwan
Prior art keywords
sodium chloride
acetate
wash buffer
cation exchange
wash
Prior art date
Application number
TW109138883A
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English (en)
Chinese (zh)
Other versions
TW202132323A (zh
Inventor
馬爾哈爾 Ｒ 安布哈卡
路易斯 狄雅茲
娜塔莉 歌梅茲
班傑明 Ｊ 提洛斯頓
Original Assignee
美商安進公司
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Publication of TW202132323A publication Critical patent/TW202132323A/zh
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Publication of TWI878377B publication Critical patent/TWI878377B/zh

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/145—Ultrafiltration
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2315/00—Details relating to the membrane module operation
    • B01D2315/16—Diafiltration
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/64—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a combination of variable region and constant region components

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Water Supply & Treatment (AREA)
  • Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
TW109138883A 2019-11-07 2020-11-06 陽離子交換層析期間之高鹽洗滌以去除產品相關的雜質 TWI878377B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962931874P 2019-11-07 2019-11-07
US62/931,874 2019-11-07

Publications (2)

Publication Number Publication Date
TW202132323A TW202132323A (zh) 2021-09-01
TWI878377B true TWI878377B (zh) 2025-04-01

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
TW109138883A TWI878377B (zh) 2019-11-07 2020-11-06 陽離子交換層析期間之高鹽洗滌以去除產品相關的雜質
TW114107974A TW202523676A (zh) 2019-11-07 2020-11-06 陽離子交換層析期間之高鹽洗滌以去除產品相關的雜質

Family Applications After (1)

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TW114107974A TW202523676A (zh) 2019-11-07 2020-11-06 陽離子交換層析期間之高鹽洗滌以去除產品相關的雜質

Country Status (14)

Country Link
US (1) US20220372071A1 (https=)
EP (1) EP4055042A1 (https=)
JP (2) JP2022554283A (https=)
KR (1) KR20220092958A (https=)
CN (1) CN114651007A (https=)
AR (1) AR120398A1 (https=)
AU (1) AU2020377919A1 (https=)
BR (1) BR112022008782A2 (https=)
CA (1) CA3160208A1 (https=)
CL (2) CL2022001169A1 (https=)
IL (1) IL292602A (https=)
MX (1) MX2022005594A (https=)
TW (2) TWI878377B (https=)
WO (1) WO2021091933A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4263597A2 (en) * 2020-12-18 2023-10-25 Kiniksa Pharmaceuticals, Ltd. Protein compositions and methods for producing and using the same

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999010370A1 (en) * 1997-08-25 1999-03-04 Torres Anthony R A simple, environmentally benign, method for purifying protein a
WO2010127069A1 (en) * 2009-04-29 2010-11-04 Schering Corporation Antibody purification
WO2018237159A1 (en) * 2017-06-21 2018-12-27 Cephalon, Inc. Cation exchange chromatography wash buffer

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2533266T5 (es) * 2007-10-30 2018-04-18 Genentech, Inc. Purificación de anticuerpos mediante cromatografía de intercambio catiónico
WO2012125735A1 (en) * 2011-03-15 2012-09-20 Abott Laboratories An integrated approach to the isolation and purification of antibodies
CN104628846B (zh) * 2013-11-06 2019-12-06 三生国健药业（上海）股份有限公司 重组蛋白质的纯化方法
WO2015198320A1 (en) * 2014-06-24 2015-12-30 Insight Biopharmaceuticals Ltd. Methods of purifying antibodies
EP3177643B1 (en) * 2014-08-04 2019-05-08 F.Hoffmann-La Roche Ag Bispecific t cell activating antigen binding molecules
EP3204415B1 (en) * 2014-10-09 2020-06-17 EngMab Sàrl Bispecific antibodies against cd3epsilon and ror1
JP6699085B2 (ja) * 2015-03-25 2020-05-27 東ソー株式会社 Ｆｃ結合性タンパク質の精製方法
EP4549463A3 (en) * 2016-06-17 2025-07-30 F. Hoffmann-La Roche AG Purification of multispecific antibodies
CN110305217B (zh) * 2018-03-27 2022-03-29 广州爱思迈生物医药科技有限公司 双特异性抗体及其应用

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999010370A1 (en) * 1997-08-25 1999-03-04 Torres Anthony R A simple, environmentally benign, method for purifying protein a
WO2010127069A1 (en) * 2009-04-29 2010-11-04 Schering Corporation Antibody purification
WO2018237159A1 (en) * 2017-06-21 2018-12-27 Cephalon, Inc. Cation exchange chromatography wash buffer

Also Published As

Publication number Publication date
US20220372071A1 (en) 2022-11-24
CL2024001792A1 (es) 2024-11-08
CA3160208A1 (en) 2021-05-14
IL292602A (en) 2022-07-01
BR112022008782A2 (pt) 2022-07-26
CL2022001169A1 (es) 2023-01-27
WO2021091933A1 (en) 2021-05-14
TW202132323A (zh) 2021-09-01
MX2022005594A (es) 2022-06-09
AR120398A1 (es) 2022-02-09
EP4055042A1 (en) 2022-09-14
TW202523676A (zh) 2025-06-16
JP2022554283A (ja) 2022-12-28
JP2026048656A (ja) 2026-03-17
KR20220092958A (ko) 2022-07-04
AU2020377919A1 (en) 2022-06-16
CN114651007A (zh) 2022-06-21

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