TWI832271B - Centrifugal tube, single-tube centrifugal device, single-tube centrifugal reaction method and single-tube centrifugation system for continuous sample - Google Patents

Abstract

The disclosure provides a centrifugal tube, a single-tube centrifugal device and a single-tube centrifugal reaction method. The centrifuge tube includes an outer tube, a cover sleeved on the outer tube to seal the outer tube, and an inner tube set in the outer tube. The inner tube includes an opening for adding reactants, a joint element for fixing the inner tube in the outer tube, a reaction tank for performing a reaction, and a liquid discharge port connecting with the reaction tank. The disclosure also provides a single-tube centrifuge device suitable for the aforementioned centrifuge tube, and a single- tube centrifugation reaction method matched with the aforementioned centrifuge tube and the single-tube centrifuge device. The disclosure also provides a single-tube centrifugation system for continuous sample matched with the aforementioned centrifuge tube and the single-tube centrifuge device.

Description

Centrifuge tubes, single-tube centrifuge devices, single-tube centrifugation reaction methods and continuous samples Single tube centrifugal system

This case relates to a centrifuge tube, a single-tube centrifugal device and a single-tube centrifugal reaction method. Specifically, the invention relates to a centrifuge tube that can perform vertical centrifugation, a centrifuge device that can perform single-tube centrifugation, and a single-tube centrifugation reaction method completed by the centrifuge tube and the centrifuge device.

With the rapid development of biotechnology, various automated analysis methods and analysis systems for large quantities of samples are constantly being introduced. The development of these technologies makes systematic sample analysis more convenient and faster. However, for some biochemical analyzes with special needs, the raw materials or consumables required may be expensive, or the samples may be difficult to obtain, making it impossible to apply the analysis system to a large number of samples.

In addition, automatic analysis systems suitable for a large number of samples require sufficient space and environment. They are usually installed in research centers or medical centers. Hospitals or research units located in rural or non-urban areas may not necessarily have sufficient space and require timely analysis. As a result, less space-consuming analysis methods and systems are needed.

On the other hand, based on different analysis objects, the number of samples taken and the delivery time may also be different. Traditional analysis methods usually require larger quantities at one time to be more economical. Therefore, a method that can flexibly correspond to the number of samples and delivery time is also needed. system.

Therefore, there is also a market demand for analysis methods suitable for small samples or continuous samples, as well as the machines and consumables required.

In order to achieve the above object, the present invention provides a centrifuge tube, which includes: an outer tube including a first accommodation space; a cover set on the outer tube to seal the outer tube; and an inner tube located in the outer tube. The first accommodation space includes: an inner tube opening for adding a reaction mixture to the inner tube, and a joint through which the inner tube is sleeved in the outer tube and allows the inner tube to be The inner tube and the outer tube of the centrifuge tube are coaxial, wherein the tube opening of the outer tube toward the bottom is the Z-axis, and the axis is the center position of the inner tube and the outer tube on a plane perpendicular to the Z-axis. The connection line; a reaction tank connected with the opening of the inner tube for accommodating the reaction mixture added from the opening of the inner tube for reaction, and a first narrow opening is included between the reaction tank and the opening of the inner tube; and a The first liquid outlet connects the reaction tank to the first accommodation space and is symmetrically arranged in a direction perpendicular to the Z-axis.

Preferably, the centrifuge tube further includes a first one-way valve disposed on the first liquid outlet. The opening and closing of the first one-way valve is controlled by a first centrifugal force. When opened, a waste gas in the reaction tank is discharged. The liquid is discharged from the reaction tank to the first accommodation space through the first liquid outlet.

Preferably, the reaction tank includes a first reaction space and a second reaction space, the first reaction space is connected with the inner tube opening, and the first reaction space and the inner tube opening include the third reaction space. A narrow opening includes a second narrow opening between the first reaction space and the second reaction space, wherein the first liquid discharge port is located in the first reaction space, and the second reaction space includes a second liquid discharge mouth.

Preferably, it further includes a first one-way valve disposed on the second liquid discharge port. The opening and closing of the first one-way valve is controlled by a first centrifugal force. When opened, a waste liquid in the reaction tank is discharged from the reaction tank. The reaction tank is discharged to the first accommodation space through the second liquid outlet.

Preferably, the centrifuge tube further includes a replenishing reagent tank, the replenishing reagent tank includes a reagent tank, a retention tank and a second one-way valve, wherein the retention tank is connected with the reaction tank.

Preferably, the second one-way valve is a mechanical valve, an electronically controlled valve or a magnetically controlled valve.

Preferably, the centrifuge tube further includes a combination of a plurality of one-way valves and drain ports arranged symmetrically based on the axis.

Preferably, the first narrow opening forms an included angle of 30-60 degrees with the Z-axis.

Preferably, the second narrow opening forms an included angle of 30 to 45 degrees with the Z-axis.

According to another object of the present invention, a single-tube centrifuge device is provided, which includes a centrifuge and a fixing member. The fixing member fixes the aforementioned centrifuge tube, wherein the centrifuge is coaxial with the centrifuge tube.

According to yet another object of the present invention, a single-tube centrifugal reaction method is provided, which includes: adding a reaction mixture to the aforementioned centrifuge tube for reaction; placing the centrifuge tube in the aforementioned single-tube centrifuge device, and fixing it by the The centrifuge tube is fixed with a piece; and waste liquid is eliminated by centrifuging the centrifuge tube.

Through the above technical features, the present invention has at least the following advantages:

(1) With the centrifuge tube according to the embodiment of the present invention, it can be operated in a single tube and can be analyzed without preparing a large number of samples.

(2) With the centrifuge tube and single-tube centrifuge device according to the embodiment of the present invention, waste liquid can be simply removed after the reaction through single-tube centrifugation, and consumables can be reduced.

(3) By combining the centrifuge tube, single-tube centrifuge device and single-tube centrifugation reaction method of the embodiment of the present invention, biochemical analysis of small samples can be completed quickly and conveniently.

1: Centrifuge tube

101:Outer tube

102: Cover

1021:Protrusion

103,103a:Inner tube

1031:Joints

1032: Reaction tank

1032a: First reaction space

1032b: Second reaction space

1033: First one-way valve

1033a:Steel ball

1033b:Spring

1034: Drain port

1034a: First drain port

1034b: Second drain port

1035: The first narrow mouth

1035a: Second narrow mouth

110: Replenish the medicine tank

111:Medicine tank

112:Reservation slot

113: Second one-way valve

2:Single tube centrifuge

201:Centrifuge

201a: Cover

202: Fixtures

203:Motor

S1~S3: steps

Figure 1 is an exploded schematic diagram of a centrifuge tube according to an embodiment of the present invention.

Figure 2 is a schematic side view of a centrifuge tube according to an embodiment of the present invention.

Figure 3 is a schematic three-dimensional view of a centrifuge tube according to an embodiment of the present invention.

Figure 4 is a schematic diagram of the liquid discharge port of a centrifuge tube according to an embodiment of the present invention.

FIG. 5 is a partially enlarged schematic view of the joint between the connecting member and the cover of the centrifuge tube according to the embodiment of the present invention.

Figure 6 is a schematic diagram of the narrow mouth of the inner tube according to an embodiment of the present invention.

Figure 7 is a schematic three-dimensional view of the inner tube 103a according to another embodiment of the present invention.

Figure 8 is a schematic three-dimensional view of the inner tube 103a according to yet another embodiment of the present invention.

Figure 9 is a schematic diagram of the closed and opened states of the replenishing medicine tank according to an embodiment of the present invention.

Figure 10 is a schematic diagram of another embodiment of a replenishing medicine tank according to an embodiment of the present invention.

Figure 11 is a schematic three-dimensional view of a single-tube centrifuge according to another embodiment of the present invention.

Figure 12 is a cross-sectional view of a single-tube centrifuge according to another embodiment of the present invention.

Figure 13 is a schematic diagram of the bottom shape of a centrifuge tube according to another embodiment of the present invention.

Figure 14 is a flow chart of a single-tube centrifugal reaction method according to an embodiment of the present invention.

Embodiments are described in detail below with reference to relevant drawings. However, these embodiments can be implemented in different forms, but this is not the only form of implementing or using the specific embodiments of the claimed invention, and therefore should not be construed as a limitation on the above-mentioned embodiments. The embodiments cover features of multiple specific embodiments as well as method steps and their sequences for constructing and operating these specific embodiments. However, other specific embodiments may also be used to achieve the same or equivalent functions and step sequences. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the spirit of the invention to those skilled in the art to which this invention belongs. Similar reference symbols in the drawings refer to similar components. In the following description, conventional functions or structures will not be described in detail to avoid unnecessary details of the embodiments.

Unless otherwise defined, all technical words and terms used herein have the same meanings commonly understood by a person of ordinary skill in the technical field to which this invention belongs. In the event of conflict, the present specification, including definitions, will control.

Unless there is conflict with the context, the singular form of a noun used in this specification shall include the plural form of the noun; and the plural form of the noun shall also include the singular form of the noun. In addition, in this specification and the scope of the patent application, the expressions "at least one" and "one or more" have the same meaning, and both of them mean that one, two, three or more are included.

The conjunction "consisting essentially of" is used to define a composition, method or device that includes materials, steps, features, components or elements other than those expressly stated, with the limitation that These additional materials, steps, features, components or elements do not materially affect the basic and novel characteristics of the claimed invention. The term "consisting essentially of" occupies a middle ground between "comprising" and "consisting of."

Notwithstanding that the numerical ranges and parameters defining the broader scope of the invention are approximations, the relevant numerical values in the specific embodiments are presented as precisely as possible. Any numerical value, however, inherently contains the standard deviation resulting from the individual testing methods used. As used herein, "about" generally means that the actual value is within plus or minus 10%, 5%, 1% or 0.5% of a specific value or range. Alternatively, the word "about" means that the actual value falls within an acceptable standard error of the mean, as determined by a person of ordinary skill in the art to which this invention belongs. Except for the examples, or unless otherwise expressly stated, it is understood that all ranges, quantities, numerical values and percentages used herein (for example, to describe the amount of material, length of time, temperature, operating conditions, quantitative proportions and other similar ) are all modified by "approval". Therefore, unless otherwise stated to the contrary, the numerical parameters disclosed in this specification and the patent scope are approximate values and may be changed according to needs. At a minimum, these numerical parameters should be understood to mean the number of significant digits indicated and the value obtained by applying ordinary rounding. Herein, numerical ranges are expressed from one endpoint to the other point or between two endpoints; unless otherwise stated, numerical ranges stated herein include the endpoints.

The centrifuge tube, single-tube centrifugal device and single-tube centrifugal reaction method of the present invention are described below through specific examples.

First, refer to FIGS. 1 to 6 . FIG. 1 is an exploded schematic diagram of a centrifuge tube 1 according to an embodiment of the present invention. Figure 2 is a schematic side view of a centrifuge tube 1 according to an embodiment of the present invention. Figure 3 is a schematic three-dimensional view of a centrifuge tube 1 according to an embodiment of the present invention. Figure 4 is a schematic diagram of the liquid discharge port 1034 of the centrifuge tube 1 according to an embodiment of the present invention. FIG. 5 is a partially enlarged schematic diagram of the joint between the joint 1031 and the cover 102 of the centrifuge tube 1 according to the embodiment of the present invention. FIG. 6 is a schematic diagram of the first narrow opening 1035 of the inner tube 103 of the centrifuge tube 1 according to an embodiment of the present invention.

The centrifuge tube 1 according to the embodiment of the present invention may include an outer tube 101, a cover 102 and an inner tube 103. The cover 102 can be set (for example, threadedly fixed) on the outside of the opening of the outer tube 101 to close the outer tube. 101. The inner tube 103 is disposed in the accommodation space formed by the outer tube 101 . The inner tube 103 may include an inner tube opening 1030, a joint 1031, a reaction tank 1032, a first one-way valve 1033, and a drain port 1034. The joint 1031 may be sleeved inside the opening of the outer tube 101. The first one-way valve 1033 may illustratively include a steel ball 1033a and a spring 1033b. In one embodiment, as shown in FIG. 5 , the cover 102 can include a protrusion 1021 at a position corresponding to the joint 1031 , which can be used when the cover 102 is sleeved (for example, fixedly sleeved with threads) on the outer tube 101 Further compress the joint 1031 to make the position of the inner tube 103 more stable.

In the following embodiments, the direction of the nozzle of the centrifuge tube 1, the outer tube 101 or the inner tube 103 toward the bottom is the Z-axis, and the line connecting the center positions of the inner tube and the outer tube on the plane perpendicular to the Z-axis is Axis (for example, ZZ' line). When the inner tube 103 is sleeved inside the opening of the outer tube 101 through the joint 1031, the inner tube 103 will be fixed in the accommodation space in the outer tube 101 in a coaxial manner with the outer tube 102, so it can be centrifuged Tube 1 remains stable during spin centrifugation. According to another embodiment of the present invention, the joint 1031 of the inner tube 103 can be sleeved on the outside of the outer tube 101. The present invention is not limited thereto, and the inner tube 103 can be fixed in the outer tube 101 in any suitable manner.

As shown in FIG. 4 , the centrifuge tube 1 according to the embodiment of the present invention includes a pair of liquid discharge ports 1034 arranged symmetrically with respect to the axis, in which a first one-way valve 1033 is provided. The first one-way valve 1033 includes steel balls 1033a and springs 1033b. When the centrifuge tube 1 is rotated and centrifuged, a pair of drain ports 1034 arranged symmetrically with respect to the axis can balance the centrifuge tube 1 and prevent it from shaking. When stopped, the steel ball 1033a in the first one-way valve 1033 in the liquid discharge port 1034 will be pushed by the spring 1033b to resist the opening connected to the reaction tank 1032 and close it. When the rotation speed increases and the centrifugal force reaches At the threshold, the centrifugal force experienced by the steel ball 1033a will be greater than the pushing force of the spring 1033b and compress the spring 1033b, thereby connecting the reaction tank 1032 to the drain port 1034, so that the waste liquid in the reaction tank 1032 can be discharged through the drain port 1034. When the centrifugal force is less than the threshold value, the steel ball 1033a will rebound due to the pushing force of the spring 1033b, thereby closing the connection between the reaction tank 1032 and the drain port 1034. The steel balls 1033a and the springs 1033b can be configured in different ways, for example, the steel balls have different weights, the springs have different elastic forces, etc., to suit different needs. In another embodiment, the connection between the reaction tank 1032 and the drain port 1034 may include a filter screen, filter paper or a semipermeable membrane.

According to an embodiment of the present invention, the centrifuge tube 1 is used with magnetic beads. In order to prevent the magnetic beads from being lost from the drain port 1034 during centrifugation, it can be blocked by the aforementioned filter screen, filter paper, etc. The top view shape of the reaction tank 1032 may be a circle or a polygon. When the top view shape of the reaction tank is a polygon, the drain port 1034 may be disposed at a symmetrical vertex of the polygon to improve the drain effect during centrifugation. According to another embodiment of the present invention, a magnetic force generating device can be used to generate magnetic force at the axis or circumference of the centrifuge tube 1 so that the magnetic beads remain in or around the centrifuge tube 1 without being discharged during centrifugation.

According to another embodiment of the present invention, the drain port 1034 may be arranged in different ways, for example, it may be 4 or 6 drain ports arranged symmetrically with respect to the axis. In addition, the first one-way valve 1033 in the drain port 1034 is not limited to a combination of steel balls and springs. It can be controlled to open and close by electronic control or magnetic control, and waste liquid can also be eliminated by centrifugal force. According to an embodiment of the present invention, the first one-way valve 1033 may be disposed in the liquid discharge port 1034 at a position close to the axis of the inner tube 103, but the present invention is not limited thereto. The first one-way valve 1033 can be disposed at a position where the liquid discharge port 1034 is away from the axis of the inner tube 103, or at any position where the one-way valve can be opened by centrifugation, electronic control or magnetic control, and then the waste liquid can be discharged.

According to an embodiment of the present invention, a first narrow opening 1035 can be provided between the reaction tank 1032 and the tube opening in the inner tube 103 . The first narrow opening 1035 is formed by being inclined toward the axis from the inner wall of the reaction tank 1032 . When the centrifuge tube 1 is centrifuged with the axis as the center, the reaction mixture in the reaction tank 1032 will move toward the inner wall of the reaction tank 1032 away from the axis due to centrifugal force. As the rotation speed increases, the centrifugal force becomes larger, causing the reaction The mixture moves along the inner wall of the reaction tank 1032 toward the pipe opening. In order to prevent the reaction mixture from overflowing from the tube opening during centrifugation, a first narrow opening can be provided between the reaction tank 1032 in the inner tube 103 and the tube opening. 1035 achieved. The first narrow opening 1035 forms an included angle of 30 to 60 degrees with the axis, preferably an included angle of 45 degrees. When centrifugation is performed and the reaction mixture moves toward the tube opening along the inner wall of the reaction tank 1032, the first narrow opening 1035 with a predetermined angle to the axis can maintain the reaction mixture in the reaction tank 1032 to prevent it from overflowing.

Next, refer to FIG. 7 , which is a schematic three-dimensional view of the inner tube 103a according to another embodiment of the present invention.

According to an embodiment of the present invention, the centrifuge tube 1 of the present invention includes an inner tube 103a. Except for the configuration of the inner tube 103a, the centrifuge tube 1 in this embodiment is the same as the previous embodiment and will not be described again.

The reaction tank 1032 of the inner tube 103a includes a first reaction space 1032a and a second reaction space 1032b, where the inner diameter of the second reaction space 1032b is larger than the first reaction space 1032a. A second narrow opening 1035a is included between the first reaction space 1032a and the second reaction space 1032b. The angle between the second narrow opening 1035a and the Z-axis may be 30 to 60 degrees, preferably 30 to 45 degrees, and more preferably 45 degrees. After the reaction mixture is added to the reaction tank 1032, it will fall into the second reaction space 1032b for reaction (it may also fill the first reaction space 1032a when the reaction mixture is large). After the reaction is completed, the centrifuge tube 1 can be centrifuged to remove waste liquid. During centrifugation, the waste liquid will move toward the side wall of the second reaction space 1032b due to centrifugal force. As the centrifugation speed increases, the waste liquid will appear in a donut shape at the side wall of the second reaction space 1032b. At the same time, the waste liquid will also be affected by the centrifugal force and move upward to the side wall of the first reaction space 1032a, and be discharged from the first liquid outlet 1034a of the first reaction space 1032a to the first volume between the outer tube 101 and the inner tube 103a. in the second reaction space 1032b, and a predetermined volume of the reaction mixture can be retained in the second reaction space 1032b. By preserving part of the liquid, aspiration can be facilitated when the reaction mixture contains magnetic beads or the like. In this embodiment, since the liquid discharge port 1034a is not provided with a valve, the waste liquid will be directly discharged into the first accommodation space. In another embodiment, the liquid discharge port 1034a can also be provided with a one-way valve as mentioned above to control the discharge of waste liquid, and the present invention is not limited thereto.

Please also refer to FIG. 8 , which is a schematic three-dimensional view of the inner tube 103a according to another embodiment of the present invention.

The configuration of the inner tube 103a in the centrifuge tube 1 of this embodiment is basically the same as that of the previous embodiment, and only the differences are described here. In this embodiment, the side wall of the second reaction space 1032b further includes a second liquid discharge port 1034b, and a one-way valve (not shown) is provided at the second liquid discharge port 1034b. After the reaction mixture completes the reaction in the second reaction space 1032b, part of the waste liquid can be discharged from the first drain port 1034a to the first accommodation space through centrifugation as described in the previous embodiment. Preferably, when all the liquid needs to be discharged, the one-way valve provided at the second liquid discharge port 1034b can be opened by controlling the centrifugal force, so that the waste liquid can be discharged from the second liquid discharge port 1034b to the first container. space. The above embodiments are only examples, and the present invention is not limited thereto. A plurality of liquid discharge ports may be provided, or different types of valves may be provided, or a filter screen, filter paper, or a semipermeable membrane may be provided at the liquid discharge port. According to yet another embodiment of the present invention, by controlling the height of the second liquid outlet 1034b in the second reaction space 1032b, the remaining amount of liquid in the second reaction space 1032b can also be controlled.

Next, please refer to FIG. 9 , which is a schematic diagram of the closed (a) and open (b) states of the replenishing medicine tank 110 according to an embodiment of the present invention. In FIG. 9 , only one side is shown as a schematic diagram, but the present invention is not limited thereto.

According to an embodiment of the present invention, the centrifuge tube 1 of the present invention may further include a replenishing agent tank 110, and the number of the replenishing agent tanks 110 may be determined according to the amount of replenishing agent used. In one embodiment, the inner tube 103 can be provided with a single replenishing medicine tank 110, and the replenishing medicine tank 110 can include a medicine tank 111, a retention tank 112, and a second one-way valve 113, wherein the second one-way valve 113 controls the medicine tank. 111 and the temporary retention tank 112, and the temporary tank 112 is connected with the inner tube 103. The opening threshold of the second one-way valve 113 may be different from that of the first one-way valve 1033 . For example, the centrifugal force threshold for opening of the second one-way valve 113 may be greater than that of the first one-way valve 1033 . The initial state of the replenishing medicine tank 110 is shown in part (a) of Figure 9. In this state, the The two one-way valves 113 resist the opening, allowing the replenishing medicine to be stored in the medicine tank 111 . After the reaction mixture passes through the inner tube 103 and is mixed in the reaction tank 1032, it can be centrifuged at a first rotation speed. At this time, the first one-way valve 1033 is opened to discharge the waste liquid of the reaction mixture from the drain port 1034. Then, the rotation speed can be accelerated to a second rotation speed greater than the first rotation speed for centrifugation. After reaching the centrifugal force threshold of the second one-way valve 113, the second one-way valve 113 is opened, and the supplementary medicine placed in the medicine tank 111 falls into the temporary storage. In the groove 112, as shown in part (b) of Figure 9 . After centrifugation is stopped, the replenishing agent falls into the inner tube 103 by gravity and reaches the reaction tank 1032, where it performs the next reaction with the reaction mixture remaining in the reaction tank 1032. On the other hand, the second one-way valve can also be electronically controlled or magnetically controlled. In addition, when the replenishing medicine tank 110 is single, a counterweight may be provided on the centrifuge tube 1 at a symmetrical position relative to the axis in a plane perpendicular to the Z-axis to achieve balance during centrifugation. Preferably, the replenishing medicine tank 110 is provided between the first narrow opening 1035 and the tube opening. According to another embodiment of the present invention, the replenishing agent tank 110 may be disposed between the first narrow opening 1035 and the reaction tank 1032 .

According to another embodiment of the present invention, a plurality of replenishing medicament tanks can be arranged symmetrically with respect to the axis in a plane perpendicular to the Z-axis on the centrifuge tube 1 . For example, a first replenishing medicine tank and a second replenishing medicine tank may be provided. The structures of the first replenishing medicine tank and the second replenishing medicine tank are similar to the structures of the aforementioned replenishing medicine tank, and respectively include the first and second replenishing medicine tanks. The first and second retention tanks respectively include a third one-way valve and a fourth one-way valve. The centrifugal force thresholds for opening the third one-way valve and the fourth one-way valve of the first replenishing medicine tank and the second replenishing medicine tank are different. In actual operation, after the reaction mixture is mixed in the reaction tank, it can be centrifuged at the first rotation speed. At this time, the first one-way valve 1033 is opened to discharge the waste liquid of the reaction mixture from the drain port 1034. At this time, the rotation speed can be adjusted to Accelerate to a third speed greater than the first speed and perform centrifugation. After reaching the centrifugal force threshold of the third one-way valve, the third one-way valve opens, and the first supplementary medicine placed in the first medicine tank falls into the first retention tank. . After centrifugation is stopped, the first replenishing agent falls into the inner tube 103 by gravity and then reaches the reaction tank 1032, where it performs the next reaction with the reaction mixture remaining in the reaction tank 1032. Then, when the opposite After the mixing reaction between the reaction mixture and the first supplementary agent is completed, centrifugation is performed at the first rotation speed. At this time, the first one-way valve 1033 is opened, and the waste liquid of the reaction mixture is discharged from the drain port 1034. Then, the rotational speed can be accelerated to a fourth rotational speed greater than the third rotational speed for centrifugation. After reaching the centrifugal force threshold of the fourth one-way valve, the fourth one-way valve is opened, and the second replenishing medicament placed in the second medicament tank falls into the third medicament tank. The second is temporarily left in the tank. After centrifugation is stopped, the second replenishing agent falls into the inner tube 103 by gravity and enters the reaction tank 1032, where it performs the next reaction with the reaction mixture remaining in the reaction tank 1032. The foregoing embodiments are only examples, and the present invention is not limited thereto. If necessary, the third and fourth one-way valves in the first and second replenishing medicament tanks may have the same or different opening centrifugal force thresholds.

According to another embodiment of the present invention, the replenishing agent tank can be combined with the centrifuge tube 1 in a layered manner. For example, as shown in FIG. 10 , the replenishing agent tank can be disposed on the centrifuge tube 1 in a nested manner. Specifically, the replenishing medicine tank of this embodiment may have the same or similar diameter as the inner tube 103 of the centrifuge tube 1 , and is provided with the aforementioned medicine tank, one-way valve, and retention tank structures in the replenishing medicine tank 110 . , where required medicaments can be added, and has a nestable fitting part (not shown). At the same time, the threshold value of the one-way valve in the replenishing medicine tank is different from the threshold value of the first one-way valve 1033 in the centrifuge tube 1 . Thereby, the required medicaments can be combined into the centrifuge tube 1 in a nested manner to achieve the effect of adding medicaments. Furthermore, when a plurality of replenishing medicine tanks are stacked, the threshold values of the one-way valves therebetween are different from each other.

In this embodiment, the replenishing medicine tank is arranged symmetrically with respect to the axis of the centrifuge tube 1 and provides different replenishing medicines in an upwardly stacked manner to avoid insufficient space on the same plane and difficulty in arranging multiple replenishing medicines. Furthermore, for a single replenishing agent, a specific proportion of concentrate and diluent of equal quality can be set in a symmetrical replenishing agent tank, so that when the one-way valve is opened and the replenishing agent flows into the temporary holding tank, the concentration can still be maintained. Equilibrate centrifuge tube 1.

Please refer to FIGS. 11 to 13 . FIG. 11 is a schematic three-dimensional view of a single-tube centrifuge 2 according to another embodiment of the present invention. Figure 12 is a cross-sectional view of a single-tube centrifuge 2 according to another embodiment of the present invention. Figure 13 is a schematic diagram of the bottom shape of the centrifuge tube 1 according to another embodiment of the present invention.

According to another object of the present invention, a single-tube centrifuge device 2 is provided. The single-tube centrifuge device 2 includes a centrifuge 201 and a fixing member 202 for fixing the centrifuge tube 1 . Centrifuge 201 may include a motor 203 for providing centrifugal force. During use, the centrifuge tube 1 is fixed on the centrifuge 201 through the fixing member 202, and different rotational speeds are provided to provide different centrifugal forces.

In one embodiment, the fixing part 202 may be provided only at the bottom of the outer tube 101 of the centrifuge tube 1 , or the fixing part 202 may also be provided on the cover 102 of the centrifuge tube 1 and the cover 201 a of the centrifuge 201 . The fixing member 202 can fix the centrifuge tube 1 in any way, such as clamping from the outside of the centrifuge tube 1 , or as shown in FIG. 13 , a groove is provided at the bottom of the centrifuge tube 1 to assist in fixing the centrifuge tube 1 to the fixing member 202 . The groove provided at the bottom of the centrifuge tube 1 can be in a straight shape, a cross shape, a quadrangular shape, a polygonal shape, or any suitable shape that can be stably fixed to the centrifuge 201 . In this embodiment, since the rotation of the centrifuge tube 1 is powered by the motor 203, the fixing member 202 connected to the bottom of the centrifuge tube 1 will firmly fix it to the centrifuge 201. In contrast, the fixing member 202 on the cover 201a of the centrifuge 201 can have a corresponding fixing structure on the cover 102 of the centrifuge tube 1, and can be stabilized in the single-tube centrifuge without affecting the centrifugation of the centrifuge tube 1. within device 2. In another embodiment, the centrifuge can accommodate multiple (ie more than one) centrifuge tubes 1 at the same time, and each centrifuge tube 1 can be independently fixed to the centrifuge and centrifuged independently without the need for a counterweight.

According to an embodiment of the present invention, a continuous sample single-tube centrifugation system can be provided, which includes a carrier; a single-tube centrifuge disposed on the carrier; a track for carrying the movement of the carrier; and an identification device disposed on the track. unit; and a control module that controls the movement of the stage and controls the centrifugation of the single-tube centrifuge. That , the single-tube centrifuge is the single-tube centrifuge described in the previous embodiment, and it is suitable for the centrifuge tube described in the previous embodiment.

According to an embodiment of the present invention, when a user receives a sample and uses the continuous sample single-tube centrifugation system, the sample and reagents are injected into the centrifuge tube, and an identification mark is given for identification by the identification unit. The user places the centrifuge tube on the single-tube centrifuge on the stage on the track, and moves the stage through the control module to perform centrifugation, adding reagents and other steps at different positions, and identifies the centrifuge tube through the identification unit identification mark on the analyzer to confirm the progress of the analysis. The identification mark can be a barcode, QR code or any identifiable mark. In another embodiment according to the present invention, required replenishment reagent tanks can be stacked, thereby eliminating the need for additional equipment for adding reagents. Preferably, this system can further include heating, cooling, shaking and other equipment to meet different experimental needs. In this way, through multiple stages on the track and the single-tube centrifuge on it, the sample can be controlled to move on the track, and different reaction steps can be performed at different positions without waiting for the sample to reach a certain number before starting analysis. Through the automatic control system control, steps such as reagent addition are also achieved by replenishing the reagent tank. Different reagents can be added by controlling different rotation speeds, saving a lot of manpower and material resources.

According to another object of the present invention, a single-tube centrifugal reaction method is provided, as shown in Figure 14, including: step S1, adding the reaction mixture into the centrifuge tube for reaction. After the reaction mixture is added to the inner tube 103, it will pass through the first narrow opening 1035 and fall into the reaction tank 1032 for reaction; step S2, place the centrifuge tube 1 in the single-tube centrifuge device 2, and fix the centrifuge tube with the fixing member 202 1; and step S3, centrifuge by centrifuge 201 to react or remove waste liquid. Depending on the experimental method, the step of releasing the medicine in the replenishing medicine tank 110 may be further included, and the purpose of releasing the medicine may be achieved by changing the rotation speed of the centrifuge 201 .

The above is merely illustrative and not restrictive. Any equivalent modifications or changes that do not depart from the spirit and scope of the present invention shall be included in the scope defined by the patent application.

1: Centrifuge tube

101:Outer tube

102: Cover

103:Inner tube

1031:Joints

1032: Reaction tank

1033: First one-way valve

1034: Drain port

Claims (13)

A centrifuge tube, including: an outer tube, including a first accommodation space; a cover, set on the outer tube to seal the outer tube; and an inner tube, located in the first accommodation space, and containing : an inner tube opening for adding a reaction mixture into the inner tube; a joint, through which the inner tube is sleeved in the outer tube and connects the inner tube of the centrifuge tube with the outer tube The tube is coaxial, with the tube opening of the outer tube toward the bottom being the Z-axis, and the axis being the line connecting the center positions of the inner tube and the outer tube on a plane perpendicular to the Z-axis; a reaction tank, and The inner tube opening is connected to accommodate the reaction mixture added from the inner tube opening for reaction, and the reaction tank and the inner tube opening include a first narrow opening; and a first liquid outlet to allow the reaction The groove is connected with the first accommodation space and is symmetrically arranged on the side wall of the inner tube in a direction perpendicular to the Z-axis. The centrifuge tube according to claim 1, further comprising a first one-way valve disposed on the first liquid outlet, and a first centrifugal force is used to control the opening and closing of the first one-way valve. When opened, the reaction tank A waste liquid in the reactor is discharged from the reaction tank to the first accommodation space through the first drain port. The centrifuge tube of claim 1, wherein the reaction tank includes a first reaction space and a second reaction space, the first reaction space is connected to the opening of the inner tube, and the first reaction space is connected to the inner tube. The first narrow opening is included between the openings, and a second narrow opening is included between the first reaction space and the second reaction space, wherein the first liquid outlet is located in the first reaction space, and the second reaction space includes A second liquid outlet. The centrifuge tube according to claim 3, further comprising a first one-way valve disposed on the second liquid outlet, and a first centrifugal force is used to control the opening and closing of the first one-way valve. When opened, the reaction tank A waste liquid in the reaction tank is discharged to the first accommodation space through the second liquid outlet. The centrifuge tube as described in claims 1 to 4, further comprising a replenishing reagent tank, the replenishing reagent tank includes a reagent tank, a retention tank and a second one-way valve, wherein the retention tank and the reaction tank Connected. The centrifuge tube according to claim 5, wherein the second one-way valve is a mechanical valve, an electronically controlled valve or a magnetically controlled valve. The centrifuge tube according to claim 6 further includes a combination of a plurality of one-way valves and drain ports arranged symmetrically based on the axis. The centrifuge tube according to claim 1, wherein the first narrow opening and the Z-axis form an included angle of 30 to 60 degrees. The centrifuge tube as described in claim 3 or 4, wherein the second narrow opening and the Z-axis form an included angle of 30 to 45 degrees. A single-tube centrifuge device includes a centrifuge and a fixing member, which fixes the centrifuge tube as described in claims 1 to 9, wherein the centrifuge is coaxial with the centrifuge tube. A single-tube centrifugal reaction method, including: adding a reaction mixture to the centrifuge tube as described in any one of claims 1 to 9 for reaction; placing the centrifuge tube in the single-tube centrifuge device as described in claim 10 , and fix the centrifuge tube through the fixing member; and centrifuge the centrifuge tube to carry out reaction or eliminate waste liquid. A continuous sample single-tube centrifugation system includes: A carrier; the single-tube centrifuge device as described in claim 10, is disposed on the carrier; a track is used to carry the movement of the carrier; an identification unit is disposed on the track; and a control module, The stage is controlled to move and the single-tube centrifuge device is controlled to centrifuge. The continuous sample single-tube centrifugation system as claimed in claim 12, wherein the single-tube centrifugation device includes an identification mark, and the identification unit recognizes the identification mark.

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