TWI818227B - Mycorrhizas embedded microbead and manufacturing method and utilizing method thereof - Google Patents

Abstract

The invention discloses a mycorrhizal microbead, which is suitable for being installed in a hydroponic device, wherein the mycorrhizal microbead includes a penetrable waterproof capsule and several mature mycorrhizae embedded in the penetrable waterproof capsule. Fungal spores. The invention also discloses a method for using mycorrhizal microbeads, which includes the following steps: (a) arranging several mycorrhizal microbeads in a hydroponic device; and (b) implanting plants with the mycorrhizal microbeads. Microbead hydroponic setup.

Description

Hydroponic device and mycorrhizal microbeads and methods of making and using the same

The present invention relates to the field of hydroponic cultivation, and in particular to a hydroponic device, mycorrhizal microbeads and methods of making and using the same. The mycorrhizal microbeads have various benefits such as promoting plant growth.

With the development of urbanization, the global population has a tendency to move densely to cities. With the pollution and reduction of agricultural land, and many consumers want to buy crops that limit the use of pesticides and fertilizers, the practice of growing crops using hydroponics has become gradually became widely adopted.

It is known that most plant species form symbiotic relationships with mycorrhizal fungi when growing in natural soil. This fungus can resist pathogens in the soil (George et al., 1995), help plants absorb essential and non-essential growth nutrients, resist drought, and promote photosynthetic pigments in chloroplasts (Leung et al., 2010). Play an extremely important role. It plays an irreplaceable role in promoting phosphorus absorption (Smith and Read, 1997), so its properties are increasingly used in the agricultural industry.

At the same time, although hydroponic cultivation provides an opportunity for sustainable agricultural development, it also suffers from different pathogens in plants such as Pythium spp (McGehee et al., 2018), Colletotrichum spp (Wan et al., 2018), Pseudomonas spp (Ke et al., 2018) al., 2019), Fusarium spp (Chinta et al., 2014), Erwinia spp (Schuerger and Batzer, 1994) and Olpidium spp (Stanghellini et al., 2010) need to face the huge challenges posed by these pathogens. Generally speaking, pathogens in hydroponic cultivation can be controlled through chemical control methods (Zinnen, 1988), but most edible and medicinal plant species, such as Artemisia dracunculus, Mentha piperita, and spearmint (Mentha), oregano (Origanum vulgare), basil (Ocimum basilicum), sage (Salvia officinalis), stevia (Stevia rebaudiana), goat mint (Melissa officinalis), rosemary (Rosmarinus officinalis), lettuce ( Lactuca sativa), spinach (Spinacia oleracea), cabbage (Brassica chinensis), tomato (Solanum lycopersicum), peppers (Capsicum), courgettes (Cucumis sativus) and celery (Apium graveolens) will therefore accumulate inorganic contaminants (such as metals) or Organic contaminants (such as pesticides or insecticides) (Kumar et al., 2018). On the other hand, various pathogens inevitably exist in the nutrient solution, which causes the unstable state of phosphate in the solution and promotes the formation of different types of phosphate compounds, which affects plant growth (Takeno et al., 2005 ; Weeks and Hettiarachchi, 2019).

In order to provide nutrients in a usable form for aquaculture plants without invasion of pathogens, mycorrhizal inoculation becomes an option for plants. But in the past, mycorrhizal research has mainly focused on aeroponic techniques, in which mycorrhizal water droplets are sprayed on plant roots. However, both symbiotic efficiency and spore production rates in water are controversial and are greatly affected by the lack of carrier substrate. In addition, poor mycorrhizal growth has also been found in nutrient solutions under low oxygen conditions (Moreira et al., 2018), because oxygen is an essential element for mycorrhizal growth and interacts with other bacteria (including pathogens) in the solution. Interspecific competition for dissolved oxygen. Therefore, how to promote an efficient symbiotic relationship between fungi and plants in hydroponic cultivation by introducing mycorrhizal bacteria under limited oxygen supply is very important.

An object of the present invention is to provide a hydroponic device and mycorrhizal microbeads and their In the production and use method, using the mycorrhizal microbeads when planting hydroponic plants can promote the infection of mycorrhizal fungi in the roots of the plants, thereby promoting the growth of roots, seedlings and leaves of the plants.

Another object of the present invention is to provide a hydroponic device and mycorrhizal microbeads and methods of making and using the same, wherein the mycorrhizal bacteria in the mycorrhizal microbeads can germinate in the nutrient solution and form external hyphae, Therefore, it is not disturbed by water flow and has better ability to symbiosis with plants.

One object of the present invention is to provide a hydroponic device and mycorrhizal microbeads and methods of making and using the same, wherein the use of the mycorrhizal microbeads can make the roots of hydroponic plants that are not in direct contact with the mycorrhizal microbeads also Can acquire mycorrhizal infection.

Another object of the present invention is to provide a hydroponic device, mycorrhizal microbeads and methods of making and using the same, wherein using the mycorrhizal microbeads when planting hydroponic plants will promote the plant's response to phosphorus (including total Phosphorus and water-soluble phosphorus) absorption.

Another object of the present invention is to provide a hydroponic device and mycorrhizal microbeads and methods of making and using the same, wherein using the mycorrhizal microbeads when planting hydroponic plants will help the plants obtain soluble nutrients. .

Another object of the present invention is to provide a hydroponic device and mycorrhizal microbeads and methods of making and using the same, wherein using the mycorrhizal microbeads when planting hydroponic plants will promote the plants to contain more carotene. , chlorophyll a and chlorophyll b, and thus promote its photosynthesis and nutritional value.

Another object of the present invention is to provide a hydroponic device and mycorrhizal microbeads and methods of making and using the same, wherein the use of the mycorrhizal microbeads when planting hydroponic plants can infect the roots of the plants. Bringing several probiotic strains to collect on the roots of the plant to form a biofilm, thus providing the plant with additional protection from being infected by other pathogens or attack.

Another object of the present invention is to provide a hydroponic device, mycorrhizal microbeads and methods of making and using the same, wherein the mycorrhizal microbeads are easy to make and low in cost.

In order to solve the above problems, the present invention proposes a mycorrhizal microbead, which is suitable for being installed in a hydroponic device, wherein the mycorrhizal microbead includes: a penetrable waterproof capsule and several embedded in the wearable Permeable water-resistant capsule of mature mycorrhizal fungus spores.

According to an embodiment, the penetrable waterproof capsule is made of waterproof material and has a plurality of penetrable holes on its surface.

According to one embodiment, the hydroponic device is used for cultivating a plant selected from the group consisting of Artemisia dracunculus, Mentha piperita, Mentha, Origanum vulgare, and Octmum basilicum. , Sage (Salvia officinalis), Stevia (Stevia rebaudiana), Western goat mint (Melissa officinalis), rosemary (Rosmarinus officinalis), lettuce (Lactuca sativa), spinach (Spinacia oleracea), cabbage (Brassica chinensis), Crops from the group consisting of tomatoes (Solanum lycopersicum), peppers (Capsicum), courgettes (Cucumis sativus) and celery (Apium graveolens).

According to another aspect of the present invention, the present invention also proposes a method for making mycorrhizal microbeads, which includes the following steps:

S010: Prepare a predetermined amount of mycorrhizal inoculum; and

S020: Embed the mycorrhizal inoculum into a penetrable waterproof capsule.

According to an embodiment, step S010 further includes the following steps:

S011: Cut the roots of plants with mycorrhizal infection into slices;

S012: Mix the flaky plant roots with high-pressure (0-250kPa) steam sterilization sand;

S013: Plant seedlings in high-pressure (0-250kPa) steam sterilized sand mixed with plant roots; and

S014: After a predetermined amount of mature spores are formed, it will be used as mycorrhizal inoculum.

According to one embodiment, the mycorrhizal microbeads are adapted to be disposed in a hydroponic device, wherein the hydroponic device is used for cultivating selected from the group consisting of Artemisia dracunculus, Mentha piperita, Green Mentha, Oreganum vulgare, Ocimum basilicum, Salvia officinalis, Stevia rebaudiana, Melissa officinalis, Rosmarinus officinalis, Lettuce (Lactuca sativa), spinach (Spinacia oleracea), cabbage (Brassica chinensis), tomato (Solanum lycopersicum), pepper (Capsicum), courgette (Cucumis sativus) and celery (Apium graveolens).

According to another aspect of the present invention, the present invention also proposes a method for using mycorrhizal microbeads, which includes the following steps:

(a) disposing several mycorrhizal microbeads in a hydroponic device; and

(b) Plants are implanted into a hydroponic device provided with the mycorrhizal microbeads.

According to one embodiment, the plant of step (b) is selected from the group consisting of Artemisia dracunculus, Mentha piperita, Mentha, Origanum vulgare, and Ocimum basilicum. , Sage (Salvia officinalis), Stevia (Stevia rebaudiana), Western goat mint (Melissa officinalis), rosemary (Rosmarinus officinalis), lettuce (Lactuca sativa), spinach (Spinacia oleracea), cabbage (Brassica chinensis), The group consisting of tomatoes (Solanum lycopersicum), peppers (Capsicum), courgettes (Cucumis sativus) and celery (Apium graveolens).

According to another aspect of the present invention, the present invention also proposes a hydroponic device, wherein the hydroponic device includes a container and a plurality of mycorrhizal microbeads, wherein the mycorrhizal microbeads include: a penetrable A waterproof capsule and a plurality of mature mycorrhizal fungus spores embedded in the penetrable waterproof capsule.

According to an embodiment, the penetrable waterproof capsule is made of waterproof material and has a plurality of penetrable holes on its surface.

1: Mycorrhizal microbeads

10: Penetrable waterproof capsule

11: Penetrable holes

20:Mycorrhizal fungus spores

1000: Hydroponic device

100: Container

101: Culture medium

Figure 1 is a schematic diagram of mycorrhizal microbeads according to a preferred embodiment of the present invention.

Figure 2 is a table showing the effects of different mycorrhizal bacteria types on the growth performance of hydroponic plants in the experiment.

Figure 3 is an arrangement diagram of mycorrhizal microbeads used in experiments of the present invention.

Figure 4 is a photo of the growth performance of plants in the experiment of the present invention.

Figure 5 is an ultraviolet-visible light spectroscopic transmission spectrum of carotene, chlorophyll a and chlorophyll b extracted from experimental plants with acetone solution in the experiment of the present invention.

Figure 6 is a bar graph of the concentrations of carotene, chlorophyll a and chlorophyll b in each group of hydroponic plants in the experiment of the present invention.

Figure 7 is a flow chart of a method for manufacturing mycorrhizal microbeads according to a preferred embodiment of the present invention.

Figure 8 is a hydroponic device according to a preferred embodiment of the present invention.

The following is a description of the implementation of the present invention through specific embodiments. Those familiar with this art can easily understand other advantages and advantages of the present invention from the content disclosed in this specification. effect.

The following describes the embodiments of the present invention with reference to the drawings. It should be noted that the following drawings are simplified schematic diagrams and only illustrate the basic concept of the present invention in a schematic manner. Therefore, the drawings only illustrate the structures related to the present invention. Rather than drawing according to the number, shape and size of the units in actual implementation, the type, quantity and proportion of each unit in actual implementation are not limited to those shown in the diagram and can be changed according to actual design needs, which are explained in advance.

Referring to Figure 7, in order to achieve the above and other objects and advantages, a preferred embodiment of the present invention provides a mycorrhizal microbead, and its production method includes the following steps:

S010: Prepare a predetermined amount of mycorrhizal inoculum; and

S020: Embed the mycorrhizal inoculum into a penetrable waterproof capsule.

The step S010 further includes the following steps:

S011: Cut the roots of plants with mycorrhizal infection into slices;

S012: Mix the flaky plant roots with high-pressure steam sterilized sand;

S013: Plant seedlings using the high-pressure steam sterilized sand mixed with sheet plant roots; and

S014: After a predetermined amount of mature spores are formed, it will be used as mycorrhizal inoculum.

Referring to FIG. 1 , the mycorrhizal microbeads 1 produced according to the method for making mycorrhizal microbeads according to the above preferred embodiment of the present invention are made by embedding several mature mycorrhizal spores 20 into a penetrable waterproof capsule 10 In other words, several mature mycorrhizal fungus spores 20 are wrapped in the penetrable waterproof capsule 10 , wherein the penetrable waterproof capsule 10 is made of waterproof material and has a plurality of penetrable holes 11 on its surface. , wherein the penetrable holes 11 allow hyphae to penetrate, so that the hyphae after germination of the mycorrhizal mycospore 20 pass through the penetrable holes 11 and extend outward, while passing through the penetrable holes 11 . hole The hyphae in the holes 11 can also obtain certain support and auxiliary fixation effects, even if the mycorrhizal microbeads 1 are not completely fixed.

Referring to Figure 8, a hydroponic device 1000 according to a preferred embodiment of the present invention includes a container 100, and several or a predetermined amount of the mycorrhizal microbeads 1 disposed in the container 100. The hydroponic device can then add the culture liquid 101 and the seedlings of the crops that are desired to be planted as needed. Preferably, the crop may be selected from the group consisting of Artemisia dracunculus, Mentha piperita, Mentha, Origanum vulgare, Ocimum basilicum, Salvia officinalis, Stevia (Stevia rebaudiana), Melissa officinalis, rosemary (Rosmarinus officinalis), lettuce (Lactuca sativa), spinach (Spinacia oleracea), cabbage (Brassica chinensis), tomato (Solanum lycopersicum), pepper (Capsicum) ), courgette (Cucumis sativus) and celery (Apium graveolens).

Specifically, the mycorrhizal microbeads according to the preferred embodiment of the present invention are produced by the following method, and the prepared mycorrhizal microbeads are subsequently used in experiments. Those skilled in the art should understand that the specific materials, species, portions, time and other details used in the following methods are only for illustration, so that those skilled in the art can understand a method of implementing the present invention and verify the effectiveness of the experiments performed by the present invention. nature, during actual implementation, the relevant specific details can be changed or replaced within the spirit and/or scope of the present invention and still achieve the same or similar technical effects as the present invention, and the implementation of the changes or replacements should still be within the spirit and/or scope of the present invention. The scope of the invention is not limited hereby.

According to the method for making mycorrhizal microbeads according to the preferred embodiment of the present invention, it is first necessary to prepare a sufficient amount or a predetermined amount of mycorrhizal inoculum, mainly mature spores (vesicles) of mycorrhizal fungi. The method is partially based on Leung et al. , (2006) then made some modifications to establish mycorrhization in pollution-free soil. Increase fungal growth. Corn (Zea mays) grown in autoclaved sand was used as the host plant and root samples of C. dactylon (which had the highest mycorrhizal infection) were used for mycorrhization. First, 10 grams of root samples were cut into small pieces and mixed with high-pressure steam sterilized sand, and then planted with corn (Zea mays) seedlings in 2-liter planting pots (Del Val et al., 1999). In preliminary experiments, by examining the roots of Zea mays at different time intervals, it was observed that a sufficient or predetermined amount of mature spores (vesicles) had been formed after 5 weeks. It is worth mentioning that the high pressure in the present invention refers to the pressure of 0-250kPa, which can preferably be implemented as 90-220kPa.

Next, referring to Figures 1 and 3, microbeads are prepared by inserting the above-mentioned mature spores (vesicles) into a permeable waterproof capsule to prepare microbeads. The mature spores and fruiting bodies are in a dormant state in the capsule until use. Soak the microbeads in water to activate the mature spores in them to enter the mycorrhizal form, transforming from mature spores (vesicles) to the state of mycelium to exert their effects.

In order to confirm that the mycorrhizal microbeads according to the present invention have the above and other advantages, the inventors conducted the following hydroponic experiments.

First, lettuce was grown into seedlings from seed germination, in which the lettuce seeds were soaked in deionized water for 30 minutes and placed on cotton wool. After 5-7 days, the seeds germinate, and then use 20% Hoagland's solution to water the seedlings.

Then, the average length and weight of the seedlings (three weeks old) were selected and transplanted into black plastic boxes containing 1.5 liters of 20% Hoagland culture medium. They were divided into three groups, namely Group C: without mycorrhizal inoculation. Group M: those with circulating mycorrhizae; and Group M+M: those using non-circulating mycorrhizae embedded with waterproof microbeads. The structure of the non-flowing mycorrhizae in the microbeads was examined by scanning electron microscopy (SEM). In addition, the solutions were maintained with dissolved oxygen at 6.5 mg/L and pH 6.0 for 28 days. All boxes were reweighted every two days with autoclaved steam-sterilized distilled water. This setting randomly sets the box to a temperature In a greenhouse at 18-25°C and a relative humidity of 60-80%, the results are shown in Figure 4. The left side is based on only Hoagland culture medium without adding any mycorrhizal bacteria; the middle is mobile mycorrhizal bacteria; On the right is a stagnant group of mycorrhizal bacteria embedded in microbeads.

Then, plant analysis is performed. First, harvest the seedlings, rinse the seedlings with deionized water during harvesting, and then divide the seedlings into two groups: seedlings and roots to measure the number of leaves and roots, and the length of the seedlings and roots. The results are shown in the table in Figure 2, which shows The effect of different mycorrhizal fungus types on the growth performance of hydroponic plants in the experiment was studied. Group C was the control group without any treatment; group M was the mobile mycorrhizal fungus group; and M+M group was the embedded mycorrhizal fungi. No-flow set of microbeads, where n=3. The plant material (seedlings and root tissue) was also analyzed for total phosphorus content (using the Molybdenum blue method) (Allen, 1989).

To confirm mycorrhizal infection, the roots of the seedlings will be flushed with deionized water to remove any solution attached to the roots. Transverse rootlets were cut into 1 cm pieces and stained with lactophenol blue. The stained roots were then placed on glass slides (10 root segments per slide) for examination under the removable eyepiece crosshairs of a compound light microscope (x100). Locations were randomly selected to estimate the percentage of mycorrhizal colonization for each sample (Requena et al., 1996).

In addition, the carotene, chlorophyll a and b contents present in fresh leaves were extracted with 80% liquid acetone at pH 7.8 containing 2.5mM sodium phosphate buffer (Porra et al., 1989), and measured immediately after extraction using a UV spectrophotometer. The results are shown in Figures 5 and 6. The amounts of these pigments were calculated quantitatively (in mg/g) according to the following formula (Lichtenthaler and Wellburn, 1983 and Wellburn, 1994):

Carotene concentration = {(1000 x absorbance at 479.8nm)-3.27[chlorophyll a concentration]-104 [Chlorophyll b concentration]}/198;

Chlorophyll a concentration = 1.75 x (absorbance at 665.6 nm) - 2.35 x (absorbance at 431.5 nm); and chlorophyll b concentration = 18.61 x (absorbance at 647.6 nm) - 3.96 x (absorbance at 460.3 nm).

Finally, the data were subjected to statistical analysis, in which the data were subjected to one-way ANOVA using SPSS 16.0 software to calculate the mean and standard deviation of each three repeated values. These averages were compared using Duncan's Multiple Range test at the 0.05 significance level. Among them, group C is the control group; group M is the flowing mycorrhizal bacteria group; and group M+M is the non-flowing group with mycorrhizal bacteria embedded in microbeads. For the same photosynthetic pigment, there are significant differences between different groups using Duncan's Multiple Range test at the 0.05 significance level. In each column of different treatments, the differences between the average numbers in the same letters are The difference is not significant compared to other treatments.

Referring to Figures 2, 5, and 6, which show the effect of mycorrhizal infection on the growth performance of plants, it can be found that mycorrhizal colonization significantly promoted the growth of experimental plants, while other treatments showed growth inhibition. In addition, the highest data for roots, leaves, root length, and seedling length were also found in the M+M group, which showed that the infected plants were well adapted to the hydroponic state in a controlled environment.

Furthermore, the experimental results also showed that there was almost no mycorrhizal infection in the roots of the plants in the control group, while the inoculated plants achieved an infection rate ranging from 16.2% to 36.4% (total root length) in the root zone of the infected plants. Among them, the M+M group had a higher mycorrhizal infection rate than the M group, which showed that compared with the M group, the symbiotic relationship between the plants and mycorrhizae in the M+M group was greatly promoted. Interestingly, all three mycorrhizal forms were found only in infected plants in the M+M group.

Referring to Figure 2, in infected plants generally there is greater High phosphorus concentration. Plant growth in the M+M group showed significant differences (<0.05) in phosphorus uptake compared to the M group, and as biomass increased, the phosphorus concentration of the plants in the M+M group increased significantly.

Figure 5 depicts the UV-visible spectroscopic transmission spectra of photosynthetic pigments in experimental plants. The spectrum specifically shows the appropriate wavelengths of the corresponding photosynthetic pigments present in the experimental plants, where the specific absorption wavelengths corresponding to carotene, chlorophyll a and chlorophyll b are 479.8 nm, 431.5 and 665.6 nm, and 460.3 and 647.6 nm, respectively. In addition, Figure 6 shows that the concentration ranges of carotene, chlorophyll a, and chlorophyll b in the experimental plants are 0.27-0.57 mg/g, 0.12-0.82 mg/g, and 0.23-0.78 mg/g, respectively. Generally speaking, this means that regardless of the type of photosynthetic pigments, these two plants show that the plants in the M+M group have higher mycorrhizal infection and significantly higher photosynthetic pigment concentrations compared to other groups.

The above experimental results show that the fungal structure embedded in the microbeads is active, which is reflected in the high overall colonization rate of mycorrhizal fungi in the plant roots and even in the parts of the plant immersed in the nutrient solution, and was found on the plants in this experiment. Mycorrhizal structures are similar to those in plants growing in soil. What's more, these mycorrhizal plants show no symptoms of plant disease on their leaf surfaces. Whereas, traditionally, due to limitations of available oxygen underwater, plants far away from mycorrhizae in the same container are not colonized by mycorrhizae, in this experiment it was highlighted that the mycorrhizae of the microbeads germinate in the nutrient solution and form external hyphae, including Distributive hyphae and absorptive hyphae grow towards the root surface through such a structure and thus adhere to the root surface without being disturbed by water flow. Mycorrhizal infection of the plant then causes the plant to increase the number of leaves and maximize its photosynthetic capacity, and its photosynthetic pigment concentration also increases significantly.

In addition, the phosphorus concentration in mycorrhizal plants also increased significantly. The experiment revealed the growth simulation effect of mycorrhizal infected roots in the M+M group compared with other groups. Among them, the hydroponic mycorrhizal plants One particular advantage is that mycorrhizae have access to soluble macronutrients, such as phosphorus in nutrient solutions, and this mechanism shows that mycorrhizal hyphae often bring benefits to the host. When the microbeads according to the preferred embodiment of the present invention can directly contact the root system of the plants, the M+M group in the experiment has 36.5% colonization and the M group has 16.2%. These mycorrhizal colonization can specifically bring several probiotic strains to the roots to form a biofilm (Noirot-Gros et al., 2018) and thus provide the plant with additional protection from attacks by other pathogens.

These results show that by providing mycorrhizal-embedded microbeads according to preferred embodiments of the present invention, there are indeed advantages in promoting plant growth in hydroponic cultivation and many other advantages, and further specifically show that the practice of utilizing said microbeads is compared to Other methods of adding mycorrhizal bacteria can have a root colonization effect and maintain a considerable concentration of chlorophyll a, b and carotene in the plant for photosynthesis. Therefore, microbeads according to preferred embodiments of the present invention can indeed be used in hydroponic cultivation to enable plants to obtain essential nutrients and achieve energy balance while eliminating potential pathogens from colonizing plant roots.

In addition, according to a preferred embodiment of the present invention, the present invention also proposes a method of using mycorrhizal microbeads 1, which includes the following steps:

(a) disposing several mycorrhizal microbeads in a hydroponic device; and

(b) Plants are implanted into a hydroponic device provided with the mycorrhizal microbeads.

In the above step (a), the hydroponic device can be implemented as a hydroponic device according to conventional technology, which uses 20% Hoagland culture medium or other culture liquids. In addition, in practical applications, mycorrhizal microbeads can be directly used as the matrix, and plant seedlings can be implanted into the matrix. Mycorrhizal microbeads can also be mixed with other matrices, such as sand, fine stones, etc., and then plant seedlings can be implanted into the matrix. in the mixed matrix.

1: Mycorrhizal microbeads

10: Penetrable waterproof capsule

11: Penetrable holes

20:Mycorrhizal fungus spores

Claims (10)

A kind of mycorrhizal microbead, suitable for being arranged in a hydroponic device, wherein the mycorrhizal microbead includes: a penetrable waterproof capsule and several mature mycorrhizal spores embedded in the penetrable waterproof capsule. . The mycorrhizal microbeads of claim 1, wherein the penetrable waterproof capsule is made of waterproof material and has several penetrable holes on its surface. The mycorrhizal microbeads of claim 1, wherein the hydroponic device is used for cultivation selected from the group consisting of Artemisia dracunculus, Mentha piperita, Mentha, Origanum vulgare), basil (Ocimum basilicum), sage (Salvia officinalis), stevia (Stevia rebaudiana), goat peppermint (Melissa officinalis), rosemary (Rosmarinus officinalis), lettuce (Lactuca sativa), spinach (Spinacia oleracea) ), cabbage (Brassica chinensis), tomato (Solanum lycopersicum), pepper (Capsicum), courgette (Cucumis sativus) and celery (Apium graveolens). A method for making mycorrhizal microbeads, including the following steps: S010: Prepare a predetermined amount of mycorrhizal inoculum; and S020: Embed the mycorrhizal inoculum into a penetrable waterproof capsule. The method for making mycorrhizal microbeads as described in claim 4, wherein step S010 further includes the following steps: S011: Cut the roots of plants with mycorrhizal infection into slices; S012: Mix the flaky plant roots with high-pressure steam sterilized sand; S013: Plant seedlings using the high-pressure steam sterilized sand mixed with sheet plant roots; and S014: After a predetermined amount of mature spores are formed, it will be used as mycorrhizal inoculum. The method for making mycorrhizal microbeads as described in claim 4, wherein the mycorrhizal microbeads are suitable for being installed in a hydroponic device, wherein the hydroponic device is used for cultivating selected species from Artemisia dracunculus ), Mentha piperita, Spearmint (Mentha), Oreganum vulgare, Basil (Ocimum basilicum), Sage (Salvia officinalis), Stevia rebaudiana, Melissa officinalis , rosemary (Rosmarinus officinalis), lettuce (Lactuca sativa), spinach (Spinacia oleracea), cabbage (Brassica chinensis), tomato (Solanum lycopersicum), pepper (Capsicum), courgette (Cucumis sativus) and celery (Apium graveolens) Crops composed of groups. A method for using mycorrhizal microbeads includes the following steps: (a) disposing several mycorrhizal microbeads in a hydroponic device; and (b) Plants are implanted into a hydroponic device provided with the mycorrhizal microbeads. The method for using mycorrhizal microbeads as described in claim 7, wherein the plant in step (b) is selected from the group consisting of Artemisia dracunculus, Mentha piperita, Mentha, oregano. (Origanum vulgare), basil (Ocimum basilicum), sage (Salvia officinalis), stevia (Stevia rebaudiana), lemongrass (Melissa officinalis), rosemary (Rosmarinus officinalis), lettuce (Lactuca sativa), spinach ( Spinacia oleracea), cabbage (Brassica chinensis), tomato (Solanum lycopersicum), pepper (Capsicum), courgette (Cucumis sativus) and celery (Apium graveolens). A hydroponic device, wherein the hydroponic device includes a container and a plurality of mycorrhizal microbeads, wherein the mycorrhizal microbeads include: a penetrable waterproof capsule and several embedded in the penetrable waterproof capsule. Capsules of mature mycorrhizal fungal spores. The hydroponic device of claim 9, wherein the penetrable waterproof capsule is made of waterproof material and has a plurality of penetrable holes on its surface.

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